CN214097497U - Sample processing and detecting system - Google Patents

Abstract

The utility model relates to a sample processing and detecting system, it includes: the sample processing module is used for diluting a liquid sample and comprises a stock solution pool, a dilution pool and a liquid transfer platform; the detection analysis module is used for detecting the diluted liquid sample and comprises a photoelectric detection assembly, an enzyme membrane detection assembly, an ion detection assembly, a pipeline control valve and a power source, wherein the pipeline, the pipeline control valve and the power source are connected with the assemblies; the control system is used for controlling the sample processing module and the detection analysis module; and the display interface is used for displaying the action state of the liquid sample, the dilution multiple of the liquid sample, the real-time detection data and the data analysis curve.

Description

Sample processing and detecting system

Technical Field

The utility model belongs to a biological reaction process online sample treatment and detection area specifically relates to a sample treatment and detecting system.

Background

The biological online reaction process is a time-varying, nonlinear and complex dynamic change process. Although biotechnology has now made great progress in the fields of genetic engineering and metabolic engineering, and high-yielding strains can be obtained by inducing mutation, genetic recombination and culture, optimization of fermentation process product production by optimization control is still one of the major problems in the field of fermentation engineering, and thus, research on online biological reaction process optimization control technology and intelligent monitoring systems is increasingly gaining attention.

At present, a plurality of on-line detection devices mainly aim at physical and chemical parameters such as temperature, pH, dissolved oxygen and the like, and the detection devices can be directly placed into a biological on-line reactor such as a fermentation tank to directly measure related parameters. However, many parameters such as reducing sugar, organic acid, synthetic intermediate, etc. in the reaction process cannot be directly detected, and mainly the detection equipment is not suitable for being placed in a biological on-line reactor, or the concentration of the substance to be detected is not in the detection range.

Patent document CN 208314001U discloses in 2018, 5/15 a flow path system of a full-automatic bioprocess analyzer, which includes a sampling assembly for quantitatively collecting and transporting a liquid, an outer wall sampling needle cleaning assembly for cleaning a sampling needle, a standard liquid assembly for providing a standard liquid for the sampling assembly, a test assembly for detecting a sample to be tested transported by the sampling assembly, and a sample tray, wherein the sampling assembly includes a sampling needle and a sampling rack for controlling the movement of the sampling needle, a sampling inlet of the sampling assembly is communicated with a first clean water tank, and a standard liquid outlet of the standard liquid assembly and a test outlet of the test assembly are both communicated with a waste liquid tank. The flow path system of the full-automatic biological process analyzer provided by the document realizes automatic quantitative calibration and sample introduction, and ensures the repeatability of the use of the analyzer. However, in practical experiment, it is difficult to detect the sample solution by diluting the sample solution once to meet the detection limit requirement, and meanwhile, the detection error is easily caused by the residual liquid in the diluting pool or the pipeline.

Patent document CN 111289295 a discloses an online sampling and detecting device for biological reaction process in 2018, 12/6, which includes two functional modules, a sampling and sample-reserving module and a reaction detecting module, and a control system for collecting reaction detection data and processing and feeding back information. The utility model discloses a be suitable for biological reaction on-line measuring, nevertheless to the great liquid appearance of volume, easily cause the error because of remaining, diluting the operation.

Disclosure of Invention

The present inventors have conducted intensive studies in order to overcome the above problems. Particularly, the utility model provides a sample processing and detecting system.

The utility model adopts the technical scheme as follows:

a sample processing and detection system, comprising:

the sample processing module is used for diluting a liquid sample and comprises a stock solution pool, a dilution pool and a liquid transfer platform;

the detection analysis module is used for detecting the diluted liquid sample and comprises a photoelectric detection assembly, an enzyme membrane detection assembly, an ion detection assembly, a pipeline control valve and a power source, wherein the pipeline, the pipeline control valve and the power source are connected with the assemblies;

the control system is used for controlling the sample processing module and the detection analysis module;

and the display interface is used for displaying the action state of the liquid sample, the dilution multiple of the liquid sample, the real-time detection data and the data analysis curve.

Preferably, the stock solution pool and the dilution pool are fixed on the support, the liquid sample is transferred between the stock solution pool and the dilution pool through a liquid transfer platform, and the liquid transfer platform comprises a three-dimensional moving support and a liquid transfer syringe.

Preferably, the lower end of the pipetting injector is connected with a 1mL, 5 mL or 10mL pipette tip or sampling needle.

Preferably, the stock solution pool is connected with a purified water pipeline, a waste liquid discharge pipeline and a sample injection pipeline; and the diluting tank is provided with a purified water pipeline, a waste liquid discharge pipeline, a high-pressure gas pipeline and a pipeline connected with the detection component.

Preferably, the dilution tank is two dilution tanks.

Preferably, the two diluting tanks are constant volume diluting tanks, and constant volume pipelines are connected to the constant volume diluting tanks.

Preferably, the inner diameter of the pipe is 0.1-15 mm; the pipeline is powered by an injection pump, a peristaltic pump, a diaphragm pump, a plunger pump and/or an air pump; and the pipelines are provided with pipeline control valves which are used for controlling the opening and closing states of the pipelines.

Preferably, the photoelectric detection assembly comprises a fiber spectrometer and a syringe pump, and the detection probe of the fiber spectrometer is arranged on the syringe pump.

Preferably, the enzyme membrane detection component is one or more of a glucose enzyme membrane, a lactate enzyme membrane, a glutamate enzyme membrane and a lysine enzyme membrane component.

Further preferably, the detection range of the enzyme membrane detection component is as follows: glucose: 1-100 g/L, xylose: 1-100 g/L, lactic acid: 1-100 g/L, lysine: 1-100 g/L, glutamic acid: 1-14 g/L, ethanol: 1 to 100 g/L.

Preferably, the ion detection assembly is one or more of a pH electrode, an ammonia ion electrode, a sodium ion electrode, a potassium ion electrode and a calcium ion electrode assembly.

Further preferably, the detection range of the ion detection assembly is pH: 0-14, ammonia nitrogen: 0.1-3000 mg/L, sodium ion: 0.5-3000 mg/L, potassium ion: 0.5-3000 mg/L, calcium ion: 0.2-18000 mg/L.

The utility model effectively reduces the dilution error by performing gradient dilution through the liquid transfer platform, which is helpful to improve the detection accuracy; the diluting tank is connected with a pipeline for conveying high-pressure gas, and after the diluting tank is diluted and purified water is cleaned, the diluting tank is dried by the high-pressure gas, so that the diluting error of a next sample can be reduced; the utility model discloses in connect enzyme membrane subassembly behind the photoelectric detection subassembly, its sharing liquid appearance and standard liquid, reducible appearance liquid and standard liquid use amount effectively reduce use cost.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for the embodiments or the prior art descriptions will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive labor.

FIG. 1 is a schematic diagram of the overall structure of a sample processing and detecting system according to the present invention;

FIG. 2 is a schematic view of a flow path structure of a sample processing and detecting system according to the present invention;

FIG. 3 is a schematic structural diagram of an embodiment of a sample processing module of the sample processing and detecting system of the present invention;

FIG. 4 is a schematic structural diagram of another embodiment of a sample processing module of the sample processing and detecting system of the present invention;

FIG. 5 is a schematic view of a photoelectric detection module of a sample processing and detecting system according to the present invention;

FIG. 6 is a schematic view of the structure of an enzyme membrane detection module of a sample processing and detection system according to the present invention;

fig. 7 is a schematic structural view of an ion detection assembly of a sample processing and detection system according to the present invention;

FIG. 8 is a perspective view of one embodiment of a sample processing and detection system of the present invention;

fig. 9 is a front view of an embodiment of a sample processing and detection system of the present invention;

fig. 10 is a perspective view of an embodiment of an ion detection assembly of the sample processing and detection system of the present invention.

Description of the symbols:

1 sample processing module, 2 detection and analysis module, 3 control system, 4 display interface, 5 stock solution pool, 6 dilution pool, 7 liquid transfer platform, 8 three-dimensional moving support, 9 liquid transfer injector, 10 gun head or sampling needle, 11 photoelectric detection component, 12 enzyme membrane detection component, 13 ion detection component, 14 purified water pipeline, 15 liquid waste discharge pipeline, 16 high-pressure gas pipeline, 17 purified water bottle, 18 standard solution pool, 19 buffer liquid bottle, 20 waste liquid bottle, 21 pipeline control valve, 22 power source, 23 constant volume pipeline, 24 magnetic stirrer, 25 optical fiber spectrometer detection probe, 26 injection pump, 27 multi-channel valve, 28 enzyme membrane reaction pool, 29 enzyme membrane detection electrode, 30 ion detection pool, 31 ion electrode, 32 sample introduction pipeline, 34 liquid waste discharge pipeline, 35 ion low-standard solvent, 36 ion high-standard solvent, 37 sample introduction temperature sensor.

Detailed Description

Specific embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. While specific embodiments of the invention are shown in the drawings, it will be understood that the invention may be embodied in various forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.

It should be noted that certain terms are used throughout the description and claims to refer to particular components. As one skilled in the art will appreciate, various names may be used to refer to a component. This specification and claims do not intend to distinguish between components that differ in name but not function. In the following description and in the claims, the terms "include" and "comprise" are used in an open-ended fashion, and thus should be interpreted to mean "include, but not limited to. When an element is referred to as being "disposed on" or "connected" to another element, it can be directly connected or indirectly connected to the other element, and the following description is of the best mode for carrying out the invention and is made for the purpose of illustrating the general principles of the description and not for the purpose of limiting the invention. The protection scope of the present invention is subject to the limitations defined by the appended claims.

It will be understood that the terms "upper", "lower", "left", "right", "top", "bottom", "inner", "outer", and the like, are used in the orientation or positional relationship indicated in the drawings for convenience in describing the present invention and to simplify the description, and do not indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present invention.

For the purpose of facilitating understanding of the embodiments of the present invention, the following description will be given by way of example with reference to the accompanying drawings, and the drawings do not limit the embodiments of the present invention.

A sample processing and detection system as shown in figures 1 and 2 comprising: the sample processing module 1 is used for diluting a liquid sample and comprises a stock solution pool 5, a dilution pool 6 and a pipetting platform 7; the detection and analysis module 2 is used for detecting the diluted liquid sample and comprises a photoelectric detection component 11, an enzyme membrane detection component 12, an ion detection component 13, a pipeline connected with the components, a pipeline control valve 22 and a power source 23; a control system 3 for controlling the sample processing module 1 and the detection and analysis module 2; and the display interface 4 is used for displaying the action state of the liquid sample, the dilution multiple of the liquid sample, real-time detection data and a data analysis curve.

In particular to a sample processing and detecting system as shown in figure 1 and figure 2, which comprises a sample processing module 1, a detection and analysis module 2, a control system 3 and a display interface 4,

the sample processing module 1 comprises a stock solution pool 5, a dilution pool 6 and a pipetting platform 7, as shown in fig. 3, 4 and 8. The liquid sample is transferred between the stock solution pool 5 and the dilution pool 6 through a liquid transfer platform 7, the liquid transfer platform 7 comprises a three-dimensional moving support 8 and a liquid transfer syringe 9 connected with the three-dimensional moving support, and the lower end of the liquid transfer syringe 9 is connected with a 1mL, 5 mL or 10mL gun head or sampling needle 10. The stock solution pool 5 and the diluting pool 6 are positioned under the liquid transferring platform 7, and the liquid transferring injector 9 of the liquid transferring platform 7 samples or samples in the stock solution pool 5 or the diluting pool 6 by moving back and forth along the linear direction of the stock solution pool 5 and the diluting pool 6.

In one embodiment, as shown in fig. 8, the three-dimensional moving rack 8 comprises a switching mechanism I moving along a transverse X axis and an X axis motor thereof, a switching mechanism II moving along a longitudinal Z axis and a Z axis motor thereof, and the stock solution tank 5 and the dilution tank 6 are arranged right below the movement of the pipetting injector 9 along the X axis; in order to fix and place the stock solution tank 5 and the dilution tank 6 conveniently, the stock solution tank and the dilution tank are arranged on the same bracket. The distance of displacement is controlled by switching mechanism I and switching mechanism II, and all is equipped with position sensor on it to accurate control displacement promotes liquid-transfering syringe 9 accurate positioning.

In a specific embodiment, the pipette injector 9 is connected with 1mL, 5 mL, 10mL of tip or sampling needle 10 at the lower end, and the tip or sampling needle and its size are selected according to the volume of the sample liquid to be sucked. In order to accelerate the mixing of the dilute liquid sample, the operation can be realized by blowing through a liquid-transfering gun head or a sampling needle, or uniformly mixing through a magnetic stirrer. The utility model discloses in, be provided with magnetic stirrers 24 below diluting pool 6.

In conventional chemical and biological analysis and detection experiments, a liquid sample is often diluted and then analyzed and detected. The purpose of the dilution is to bring the detected liquid sample to the detection limit. The utility model discloses in, realize the automatic dilution of sample liquid gradient through setting up a plurality of dilution ponds, improve the dilution accuracy, reduce the dilution error. The utility model relates to a concrete implementation mode sets up and dilutes pond quantity for two, is diluting pond I6-1, diluting pond II 6-2 respectively, and the transfer of appearance liquid between stoste pond 5, diluting pond I6-1, diluting pond II 6-2 is realized through moving liquid platform 7. Specifically, the pipetting injector 9 on the pipetting platform 7 moves along the horizontal direction of the stock solution pool 5, the dilution pool I6-1 and the dilution pool II 6-2, i.e. the X axis of the switching mechanism I, when the pipetting injector is positioned right above the stock solution pool 5, the dilution pool I6-1 or the dilution pool II 6-2, the movement is stopped, at this time, the pipetting injector 9 moves downwards along the Z axis direction, and when the gun head or the sampling needle 10 enters the stock solution pool 5, the dilution pool I6-1 or the dilution pool II 6-2, the liquid sample is quantitatively sucked or injected.

In one embodiment of the present invention, the dilution pool I6-1 and the dilution pool II 6-2 are gradient dilution pools, and the dilution multiples of the dilution pool I6-1 and the dilution pool II 6-2 to the liquid sample are 10-50 times and 100-500 times respectively. In one embodiment, the dilution is 10-fold and 100-fold, respectively, and the dilution operation is as follows: feeding 18 mL of purified water such as a dilution pool I6-1 through a purified water pipeline 14, taking 2 mL of liquid sample from a stock solution pool 5 by using a pipette injector gun head 10, putting the liquid sample into the dilution pool I6-1, and uniformly mixing by using a magnetic stirrer 24-1; purified water is added in the same method, 2 mL of liquid sample is taken from the dilution pool I6-1 and is put into the dilution pool II 6-2, and the liquid sample is uniformly mixed by a magnetic stirrer 24-2.

The utility model discloses in, the purified water is pure water, sterile water, deionized water, two distilled water or ultrapure water, chooses for use according to the experiment needs. In addition, in a specific experiment, a diluting solvent may be used instead of purified water, and other solvents for dilution may be used as long as the experimental conditions permit. The utility model adopts purified water. The consumption of the purified water in the dilution pool I6-1 and the dilution pool II 6-2 is controlled by the combined action of the purified water pipeline power source and the pipeline control valve, as shown in fig. 3 and 4. In the dilution process, purified water fed by a purified water pipeline is used as a diluting solvent, and after the dilution is finished, the purified water is used for cleaning the pipeline, a stock solution pool and a diluting pool.

Specifically, the purified water inlet pipes in the raw liquid tank 5 and the dilution tank 6 in fig. 3 are independent pipes, and the consumption of the purified water is controlled by independent power sources 14-1, 14-2 and 14-3 and pipe control valves 22-1, 22-2 and 22-3.

In another specific embodiment, as shown in fig. 4, the diluting pool I6-1 and the diluting pool II 6-2 are diluting pools with constant volume structures, and the diluting pools have constant volume pipes 23. The volume of the diluting tank can be customized according to the size, wherein one mode is the same as the structure and the outer diameter of the diluting tank, and the volume of the diluting tank can be changed by changing the inner diameter; the diluting pool can be customized into different structures and outer diameters according to requirements, so that the inner diameter and the volume of contained liquid are correspondingly changed. In this embodiment, the stock solution tank 5 and the dilution tank 6 share one purified water pipeline, the amount of the purified water used as the diluting solvent in the dilution tank is realized according to the constant volume structure, and when the amount of the purified water entering is larger than the constant volume, the purified water overflows from the constant volume pipeline 23.

In one embodiment, the dilution ratio of the dilution wells I6-1 and II 6-2 to the liquid sample is 10-50 times and 100-500 times, respectively. In a specific embodiment, the dilution pool I6-1 and the dilution pool II 6-2 respectively dilute the stock solution sample by 20 and 400 times, and the specific operation steps are as follows: purified water is fed into the dilution pool I6-1, and the water inflow exceeds a constant volume pipeline, namely 38 mL of purified water is fed into the dilution pool I6-1, the control valve 21-7 of the constant volume pipeline 23-1 of the dilution pool I6-1 is closed, then 2 mL of liquid sample is taken from the stock solution pool 5 by using the gun head 10 and is put into the dilution pool I6-1, and the magnetic stirrer 24-1 is used for uniformly mixing; the diluting operation of the diluting pool II 6-2 is the same as that of the diluting pool I6-1, purified water enters the diluting pool II 6-2, the water inflow exceeds a constant volume pipeline, namely 38 mL of purified water is in the diluting pool II 6-2 at the moment, a control valve 21-4 of the constant volume pipeline 23-2 of the diluting pool II 6-2 is closed, then 2 mL of liquid sample is taken from the diluting pool I6-1 by a gun head 10 and is put into the diluting pool II 6-2, and a magnetic stirrer 24-2 is used for uniformly mixing.

The number of the dilution wells 6 in the sample processing module 1 may be 2 or more, and the number is not limited, and it is sufficient if the dilution wells are used for gradient dilution of a liquid sample. The sample processing module 1 is used for diluting and uniformly mixing the liquid sample, and the liquid sample in the diluting pool enters the photoelectric detection assembly 11, the enzyme membrane detection assembly 12 and the ion detection assembly 13 through pipelines for detection and analysis; and the pipelines are provided with pipeline control valves. The photoelectric detection component 11 comprises a fiber spectrometer and an injection pump 26, wherein a fiber spectrometer detection probe 25 is arranged on the injection pump 26, and the photoelectric detection index of the liquid sample can be detected based on a laser system. The enzyme membrane detection component 12 performs enzyme membrane detection by arranging an enzyme membrane electrode in the detection cell, wherein the enzyme membrane can be one or more of a glucose enzyme membrane, a lactate enzyme membrane, a glutamate enzyme membrane and a lysine enzyme membrane. The ion detection assembly 13 performs ion detection by arranging an ion electrode in the detection cell, wherein the electrode may be one or more of a pH electrode, an ammonia ion electrode, a sodium ion electrode, a potassium ion electrode, and a calcium ion electrode. After the liquid sample detection is completed, the residual liquid samples in the stock solution tank 5 and the dilution tank 6 enter the waste liquid discharge pipeline 15 through pipelines, air enters the left end of the waste liquid discharge pipeline 15, the right end of the waste liquid discharge pipeline is connected with a power source 22-4 close to the waste liquid bottle 20, and the power source 22-4 is further a self-sucking pump, and the peristaltic pump is a specific embodiment. After the liquid samples in the stock solution tank 5 and the dilution tank 6 are completely discharged, purified water is fed from a purified water pipeline 14 to be used for cleaning the stock solution tank 5, the dilution tank 6 and related pipelines thereof, and after the cleaning is completed, high-pressure air is fed through a high-pressure gas pipeline 16 to be used for drying the dilution tank.

More specifically, the dilution pool I6-1 or the dilution pool II 6-2 enters the photoelectric detection assembly 11 through the multi-channel valve 27, as shown in FIG. 2 and FIG. 5, the multi-channel valve 27 is connected to the photoelectric detection assembly 11, the dilution pool I6-1, the dilution pool II 6-2, the standard liquid pool 18, the waste liquid discharge pipeline 15 and the enzyme membrane detection assembly 12, and in addition, a valve communicated with the atmosphere is further arranged on the multi-channel valve 27.

More specifically, during photoelectric detection, the multi-channel valve 27 is rotated to communicate the standard liquid pool 18 with the photoelectric detection assembly 11, the injection pump 26 sucks the standard liquid from the standard liquid pool 18, the standard liquid is rinsed and then calibrated, then part of the standard liquid is pushed into the enzyme membrane reaction pool 28, and the rest of the standard liquid is pushed into the waste liquid discharge pipeline 15 by the injection pump 26; and absorbing sample liquid from the dilution pool I6-1 or the dilution pool II 6-2 for rinsing and then carrying out photoelectric detection, pushing part of the sample liquid into the enzyme membrane reaction pool 28 after detection is finished, and discharging the rest sample liquid through a waste liquid discharge pipeline 15.

More specifically, during enzyme membrane detection, a liquid inlet sample pipeline is connected above the reaction tank 28 of the enzyme membrane detection assembly 12, and the reaction tank is connected with a buffer liquid inlet pipeline, a waste liquid discharge pipeline and a constant volume pipeline. When the enzyme membrane reaction tank is in a non-working state, the buffer solution is filled in the enzyme membrane reaction tank 28, the buffer solution is discharged through a waste liquid discharge pipe at the bottom of the buffer solution, the enzyme membrane is activated by the buffer solution when the enzyme membrane reaction tank works, the sample volume of the buffer solution reaches the position of a constant volume pipeline, if the buffer solution is higher than the constant volume pipeline, the surplus buffer solution overflows from the constant volume pipeline 23-3, then the standard solution with the constant volume is subjected to enzyme membrane detection calibration, the liquid in the enzyme membrane reaction tank is discharged from the waste liquid discharge pipe at the bottom after the detection is finished, then the liquid sample is obtained, the method is the same as the standard solution and is subjected to detection, after the detection is finished, the buffer solution is fed in a buffer solution bottle 19 to clean the reaction tank, the buffer solution is filled in the reaction tank again after the cleaning is carried out for 3-4 times, and the next liquid sample detection is carried out. An enzyme membrane detection electrode 29 is arranged in the enzyme membrane reaction tank 28, and the enzyme membrane detection electrode 29 can be one or more of a glucolase membrane, a lactic acid enzyme membrane, a glutaminase membrane and a lysinase membrane. The utility model discloses in also can connect the enzyme membrane detection subassembly of setting up different enzyme membranes through multichannel valve 27. The pipelines in the enzyme membrane detection component 12 are all provided with pipeline control valves for controlling the opening and closing of the pipelines, and the buffer solution pipeline is also provided with a power source 22-6.

Further specifically, the diluting pool I6-1 is further connected with a pipeline communicated to the ion detecting component 13, the ion detecting component includes an ion detecting pool 30 and an electrode 31, the ion detecting pool 30 is provided with a sample introduction pipeline 32 for entering a liquid sample, a purified water inlet pipeline 15, a waste liquid discharge pipeline 34, a constant volume pipeline 23-4, a high pressure gas pipeline 16, and a pipeline for connecting an ion low-standard solvent 35 and an ion high-standard solvent 36, as shown in fig. 7. The ion detection cell 30 is provided with an electrode 31 which is a pH electrode 31-1 (the pH electrode can be used for detecting pH, and more importantly, the pH electrode can be used as a reference electrode for other ion detection) and other detection electrodes 31-2, the other detection electrodes 31-2 are one or more of an ammonia ion electrode, a sodium ion electrode, a potassium ion electrode and a calcium ion electrode, the ion detection cell 30 is divided into a plurality of small detection cells which are communicated with one another, as shown in fig. 7, the number of the small detection cells is two or more, the specific number is not limited, the electrodes are arranged in the small detection cells, and the structure effectively saves detection sample liquid and reagents.

Before ion detection, the ion detection cell 30 does not contain any liquid, and the detection process is as follows: firstly, cleaning, namely cleaning by feeding purified water through a purified water pipeline 14, and blowing the ion detection cell by feeding high-pressure gas through a high-pressure gas pipeline 16; secondly, calibration, namely, feeding an ion low-standard solvent 35 for calibrating low standard, discharging waste after the low standard is finished, feeding purified water for cleaning, blowing the ion detection pool 30 by high-pressure gas, feeding an ion high-standard solvent 36 for calibrating high standard, discharging waste after the detection is finished, feeding purified water for cleaning, and blowing the ion detection pool by high-pressure gas; when the liquid sample is detected, the operation is the same as the calibration detection of the low-standard solvent and the high-standard solvent, and the ion detection pool is blown dry after the detection is finished for the next detection. In the ion detection process, the opening and closing of the pipelines are controlled by respective pipeline control valves, and liquid sample power is provided according to the corresponding power source setting.

In a specific embodiment, a constant temperature module is arranged below the enzyme membrane reaction tank to ensure that the enzyme membrane reaction is carried out at a constant temperature; a temperature sensor 37 is arranged in the ion detection cell 30 and used for temperature compensation during software calculation. The utility model discloses in, the power supply is the peristaltic pump in a specific embodiment, and the pipeline control valve is the solenoid valve.

In the utility model, the pipeline is a silicone tube, a polytetrafluoroethylene tube and/or an organic plastic hose. In a specific embodiment, the pipeline for conveying the liquid sample is a polytetrafluoroethylene pipe, the material has good hydrophobicity, and the pipeline made of the material can effectively reduce waste caused by wall hanging of the liquid sample and increase sampling accuracy; common silicone tubes or organic plastic hoses are adopted for other pipelines.

In one embodiment, the inner diameter of the tube is 0.1 to 15 mm; further preferably 0.5 to 5 mm; the inner diameters of the pipelines can be completely consistent or not, and can be set to be reasonable according to requirements, and the inner diameter range can be within a set value.

In an embodiment, the utility model discloses processing module, photoelectric detection subassembly, enzyme membrane detection subassembly, control system, display interface set up in a box, the length of box is (450-; the ion detection module is disposed in another chamber, and the length, width, and height of the chamber are (250-.

In order to realize detecting automated control, the utility model discloses still including the control module who is used for controlling the appearance of advancing, it is through moving liquid platform, photoelectric detection subassembly, enzyme membrane determine module, ion detect subassembly to stoste pond, dilution pond I, dilution pond II, sample to and pipeline power supply, pipeline control valve link to each other with total control system, realize sample treatment and detecting system's automated control. The control module comprises a general processor, a digital signal processor, an Application Specific Integrated Circuit (ASIC) or a Field Programmable Gate Array (FPGA), and can be used for programming the control unit according to the sampling requirement. The control unit receives an externally input dilution signal, which comprises sample liquid dilution multiple, photoelectric detection parameters, ion detection parameters and enzyme membrane detection parameters, the control unit outputs a control signal to control a power source and a pipeline control valve, so that the liquid sample is diluted reasonably by multiple, the photoelectric detection is controlled to be detected in a whole wave band of 350-plus 800nm, the enzyme membrane detection indexes are controlled to be 1-100 g/L of glucose, 1-100 g/L of xylose, 1-100 g/L of lactic acid, 1-100 g/L of lysine, 1-14 g/L of glutamic acid and 1-100 g/L of ethanol, the ion detection indexes are controlled to be pH 0-14, 0.1-3000 mg/L of ammonia nitrogen, 0.5-3000 mg/L of sodium ions, 0.5-3000 mg/L of potassium ions and 0.2-18000 mg/L of calcium ions, and analyzing and storing the data.

Be right for making things convenient for operating personnel the utility model discloses the operation control of device, sample treatment and detecting system still includes display interface 4, like fig. 1, fig. 9, display interface can show the utility model discloses the working condition of system, for example: and displaying the dilution times, the detection parameters and the detection results, the states of the cleaning and disinfection work and the like. For convenient operation, the display interface is a display screen, and further is a touchable display screen, and display operation buttons are arranged on the display interface to realize control over the control unit.

The utility model effectively reduces the dilution error by performing gradient dilution through the liquid transfer platform, which is helpful to improve the detection accuracy; a pipeline for conveying high-pressure gas is connected in the dilution tank, purified water cleaning is carried out after dilution of the liquid sample is finished, and the dilution error of the next sample is reduced through a high-pressure gas drying pipeline; by carrying out photoelectric detection firstly and then carrying out enzyme membrane detection by using a liquid sample subjected to photoelectric detection, the use amount of the sample liquid and the standard liquid is small, and the use cost is effectively reduced.

Claims (10)

1. A sample processing and testing system, comprising:

the sample processing module is used for diluting a liquid sample and comprises a stock solution pool, a dilution pool and a liquid transfer platform;

the detection analysis module is used for detecting the diluted liquid sample and comprises a photoelectric detection assembly, an enzyme membrane detection assembly, an ion detection assembly, a pipeline control valve and a power source, wherein the pipeline, the pipeline control valve and the power source are connected with the assemblies;

the control system is used for controlling the sample processing module and the detection analysis module;

and the display interface is used for displaying the action state of the liquid sample, the dilution multiple of the liquid sample, the real-time detection data and the data analysis curve.

2. The sample processing and detecting system according to claim 1, wherein the stock solution well and the diluting well are fixed on a support, and the liquid sample is transferred between the stock solution well and the diluting well through a pipetting platform, wherein the pipetting platform comprises a three-dimensional moving support and a pipetting injector.

3. The sample processing and testing system according to claim 2, wherein said pipetting injector is terminated with a 1mL, 5 mL or 10mL tip or needle.

4. The sample processing and detecting system according to claim 1, wherein the stock solution tank is connected with a purified water pipeline, a waste liquid discharge pipeline and a sample injection pipeline; and the diluting tank is provided with a purified water pipeline, a waste liquid discharge pipeline, a high-pressure gas pipeline and a pipeline connected with the detection component.

5. The sample processing and testing system of claim 4, wherein said dilution wells are at least two dilution wells for gradient dilution.

6. The sample processing and testing system of claim 5, wherein both diluting cells are constant volume diluting cells, and a constant volume conduit is further connected thereto.

7. The sample processing and testing system according to any of claims 4 or 6, wherein said tubing has an internal diameter of 0.1-15 mm; the pipeline is powered by an injection pump, a peristaltic pump, a diaphragm pump, a plunger pump and/or an air pump; and the pipelines are provided with pipeline control valves which are used for controlling the opening and closing states of the pipelines.

8. The sample processing and testing system of claim 1, wherein said photoelectric detection assembly comprises a fiber optic spectrometer and a syringe pump, and said fiber optic spectrometer detection probe is disposed on the syringe pump.

9. The sample processing and testing system of claim 1, wherein said enzyme membrane testing component is one or more of a glucose enzyme membrane, a lactate enzyme membrane, a glutamate enzyme membrane, and a lysine enzyme membrane component.

10. The sample processing and testing system of claim 1, wherein said ion detection assembly is one or more of a pH electrode, an ammonia ion electrode, a sodium ion electrode, a potassium ion electrode, and a calcium ion electrode assembly.

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