CN214473430U - Full-automatic biological on-line measuring device - Google Patents
Full-automatic biological on-line measuring device Download PDFInfo
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- CN214473430U CN214473430U CN202022683897.6U CN202022683897U CN214473430U CN 214473430 U CN214473430 U CN 214473430U CN 202022683897 U CN202022683897 U CN 202022683897U CN 214473430 U CN214473430 U CN 214473430U
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Abstract
The utility model relates to a full-automatic biological online detection device, which comprises a sample processing module, a detection and analysis module, a control module and a display interface, wherein the sample processing module comprises an operation bin, a stock solution pool, a dilution pool and a liquid transfer platform, the stock solution pool is arranged at the bottom of the operation bin, and the detection and analysis module comprises an enzyme membrane detection component arranged in the operation bin, and a photoelectric detection component and an ion detection component outside the operation bin; the control module is connected with the liquid transfer platform, the enzyme membrane detection assembly, the photoelectric detection assembly and the ion detection assembly; the display interface is connected with the control module and used for displaying the pipetting action state, the liquid sample dilution multiple, the real-time detection data and the data analysis curve.
Description
Technical Field
The utility model belongs to a biological reaction process on-line measuring field, concretely relates to full-automatic biological on-line measuring device.
Background
The biological online reaction process is a time-varying, nonlinear and complex dynamic change process. Although biotechnology has now made great progress in the fields of genetic engineering and metabolic engineering, and high-yielding strains can be obtained by inducing mutation, genetic recombination and culture, optimization of fermentation process product production by optimization control is still one of the major problems in the field of fermentation engineering, and thus, research on online biological reaction process optimization control technology and intelligent monitoring systems is increasingly gaining attention.
At present, a plurality of on-line detection devices mainly aim at physical and chemical parameters such as temperature, pH, dissolved oxygen and the like, and the detection devices can be directly placed into a biological on-line reactor such as a fermentation tank to directly measure related parameters. However, many parameters such as reducing sugar, organic acid, synthetic intermediate, etc. in the reaction process cannot be directly detected, and mainly the detection equipment is not suitable for being placed in a biological on-line reactor, or the concentration of the substance to be detected is not in the detection range.
Disclosure of Invention
The present inventors have conducted intensive studies in order to overcome the above problems. Particularly, the utility model provides a sample processing and detecting system.
The utility model adopts the technical scheme as follows:
a full-automatic biological online detection device comprises a sample processing module, a detection and analysis module, a control module and a display interface, wherein the sample processing module comprises an operation bin, a stock solution pool, a dilution pool and a liquid transfer platform, the stock solution pool and the dilution pool are arranged at the bottom of the operation bin, and the detection and analysis module comprises an enzyme membrane detection assembly arranged in the operation bin, a photoelectric detection assembly and an ion detection assembly outside the operation bin; the control module is connected with the liquid transfer platform, the enzyme membrane detection assembly, the photoelectric detection assembly and the ion detection assembly; the display interface is connected with the control module and used for displaying the pipetting action state, the liquid sample dilution multiple, the real-time detection data and the data analysis curve.
Preferably, the pipetting platform comprises a first rotary pipetting platform and a second rotary pipetting platform, and the pipetting pipelines of the first rotary pipetting platform and the second rotary pipetting platform are positioned and pipetted along with the first rotary pipetting platform and the second rotary pipetting platform.
Preferably, the first rotary pipetting platform and the second rotary pipetting platform are both in rotary motion along the vertical axis direction and in up-and-down motion along the vertical axis direction.
Further preferably, the first rotary pipetting platform has a diameter of rotation of 15 to 18cm and the second rotary pipetting platform has a diameter of rotation of 20 to 23 cm.
Preferably, the sample transfer ports of the first rotary pipetting platform and the second rotary pipetting platform are provided with 1mL, 5mL or 10mL tips or sampling needles.
Preferably, a dilution pool is arranged at the intersection of the rotating tracks of the first rotating pipetting platform and the second rotating pipetting platform, a stock solution pool is further arranged on the rotating track of the first rotating pipetting platform, and an enzyme membrane reaction pool and a standard solution pool are further arranged on the rotating track of the second rotating pipetting platform.
Preferably, the stock solution pool, the dilution pool, the enzyme membrane reaction pool and the standard solution pool are all embedded into the bottom of the operation bin, and the two dilution pools are constant-volume dilution pools.
Preferably, the dilution tank is provided with a high-pressure air pipeline, and the bottom of the dilution tank is provided with an electromagnetic stirrer.
Preferably, the photoelectric detection assembly comprises a fiber spectrometer and a syringe pump, and the detection probe of the fiber spectrometer is arranged on the syringe pump.
Preferably, the enzyme membrane detection component is one or more of a glucose enzyme membrane, a lactate enzyme membrane, a glutamate enzyme membrane and a lysine enzyme membrane component.
Preferably, the ion detection assembly is one or more of a pH electrode, an ammonia ion electrode, a sodium ion electrode, a potassium ion electrode and a calcium ion electrode assembly.
The utility model integrates the liquid receiving and transferring platform, the enzyme membrane detection component, the photoelectric detection component and the ion detection component, and is controlled by the control system, thereby realizing the innovation of the full-automatic biological online detection device; the utility model discloses the structure is inseparable, especially moves liquid platform and move liquid platform and increased the space utilization, makes the device volume reduce greatly.
The utility model effectively reduces the dilution error by performing gradient dilution through the liquid transfer platform, which is helpful to improve the detection accuracy; the diluting pool is connected with a pipeline for conveying high-pressure gas, the liquid sample is diluted and cleaned by purified water, and the dilution error of the next sample is reduced through the high-pressure gas drying pipeline.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for the embodiments or the prior art descriptions will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive labor.
FIG. 1 is a schematic view of the overall structure of a full-automatic online biological detection device of the present invention;
FIG. 2 is a structural diagram of an embodiment of a full-automatic online biological detection device of the present invention;
FIG. 3 is a schematic view of a pipeline of an embodiment of a sample processing module of the fully automatic online biological detection device of the present invention;
FIG. 4 is a schematic view of a pipeline of a sample processing module according to another embodiment of the fully automatic online biological detection device of the present invention;
FIG. 5 is a schematic view of an embodiment of a platform structure of an operation cabin of the full-automatic online biological detection device of the present invention;
FIG. 6 is a schematic structural view of a photoelectric detection assembly of the full-automatic online biological detection device of the present invention;
FIG. 7 is a schematic view of the structure of an enzyme membrane detection assembly of the full-automatic online biological detection device of the present invention;
FIG. 8 is a schematic structural view of an ion detecting assembly of the fully automatic on-line biological detecting device of the present invention;
description of the symbols:
1 sample processing module, 2 detection and analysis module, 3 control system, 4 display interface, 5 stock solution pool, 6 dilution pool, 7 liquid transfer platform, 8 photoelectric detection component, 9 enzyme membrane detection component, 10 ion detection component, 11 sterile water pipeline, 12 liquid discharge pipeline, 13 high-pressure gas pipeline, 14 purified water bottle, 15 peristaltic pump, 16 electromagnetic valve, 17 waste liquid bottle, 18 constant volume pipeline, 19 standard solution pool, 20 first rotary liquid transfer platform, 21 second rotary liquid transfer platform, 22 first rotary track, 23 second rotary track, 24 photoelectric detection probe, 25 injection pump, 26 multi-channel valve, 27 liquid transfer pipeline, 28 enzyme membrane detection electrode, 29 buffer solution, 30 enzyme membrane reaction pool, 31 ion low-standard solvent, 32 ion high-standard solvent, 33 ion electrode, 34 sample injection pipeline, 35 liquid discharge pipeline, 36 ion detection pool, 37 magnetic stirrer, thermostat 38, 39 operates the cabin.
Detailed Description
Specific embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. While specific embodiments of the invention are shown in the drawings, it will be understood that the invention may be embodied in various forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.
It should be noted that certain terms are used throughout the description and claims to refer to particular components. As one skilled in the art will appreciate, various names may be used to refer to a component. This specification and claims do not intend to distinguish between components that differ in name but not function. In the following description and in the claims, the terms "include" and "comprise" are used in an open-ended fashion, and thus should be interpreted to mean "include, but not limited to. When an element is referred to as being "disposed on" or "connected" to another element, it can be directly connected or indirectly connected to the other element, and the following description is of the best mode for carrying out the invention and is made for the purpose of illustrating the general principles of the description and not for the purpose of limiting the invention. The protection scope of the present invention is subject to the limitations defined by the appended claims.
It will be understood that the terms "upper", "lower", "left", "right", "top", "bottom", "inner", "outer", and the like, are used in the orientation or positional relationship indicated in the drawings for convenience in describing the present invention and to simplify the description, and do not indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present invention.
For the purpose of facilitating understanding of the embodiments of the present invention, the following description will be given by way of example with reference to the accompanying drawings, and the drawings do not limit the embodiments of the present invention.
The full-automatic online biological detection device shown in fig. 1 and fig. 2 comprises a sample processing module 1, a detection and analysis module 2, a control module 3 and a display interface 4, wherein the sample processing module 1 comprises an operation bin 39, a stock solution pool 5 and a dilution pool 6 which are arranged at the bottom of the operation bin, and a liquid transfer platform 7, and the detection and analysis module 2 comprises an enzyme membrane detection component 9 arranged in the operation bin, and a photoelectric detection component 8 and an ion detection component 10 which are arranged outside the operation bin; the control module 3 is connected with a liquid transfer platform 7, an enzyme membrane detection assembly 9, a photoelectric detection assembly 8, an ion detection assembly 10 and a peristaltic pump 15 and an electromagnetic valve 16 of related pipelines thereof; the display interface 4 is connected with the control module 3 and used for displaying the pipetting action state, the liquid sample dilution multiple, the real-time detection data and the data analysis curve.
Specifically, the sample processing module 1 comprises an operation cabin 39, a stock solution pool 5 arranged at the bottom of the operation cabin, a dilution pool 6, and a pipetting platform 7, as shown in fig. 3, 4 and 5, the pipetting platform comprises a first rotary pipetting platform 20 and a second rotary pipetting platform 21, and pipetting pipelines thereof rotate and position to pipette with the first rotary pipetting platform 20 and the second rotary pipetting platform 21 respectively.
More specifically, as shown in fig. 5, a dilution pool I6-1 and a dilution pool II 6-2 are arranged at the intersection of the first rotating track 22 of the first rotating pipetting platform and the second rotating track 23 of the second rotating pipetting platform, the stock solution pool 4 is further arranged on the first rotating track 22, and the enzyme membrane reaction pool 9 and the standard solution pool 19 are further arranged on the second rotating track 23, so that the structure is compact, and the space utilization rate is increased.
The utility model discloses in, first rotatory move liquid platform 20, the rotatory appearance mouth that moves of second moves liquid platform 21 all are provided with 1mL, 5mL or 10 mL's rifle head or sampling needle. The pipette tip or the sampling needle rotates along the vertical axis direction of the first rotary pipetting platform 20 and the second rotary pipetting platform 21. When the gun head or the sampling needle reaches the stock solution pool 5, the dilution pool 6, the enzyme membrane reaction pool 9 or the standard solution pool 19 along with the first rotary pipetting platform 20 and the second rotary pipetting platform 21, the gun head or the sampling needle moves downwards along the axial direction of the first rotary pipetting platform 20 and the second rotary pipetting platform 21 so as to be immersed in the liquid sample for sampling or delivering.
In order to accelerate the mixing of the dilute liquid sample, the operation can be realized by blowing through a liquid-transfering gun head or a sampling needle or uniformly mixing through a magnetic stirrer. The utility model discloses in, be provided with magnetic stirrers 37 below diluting pool 6.
In conventional chemical and biological analysis and detection experiments, a liquid sample is often diluted and then analyzed and detected. The purpose of the dilution is to bring the detected liquid sample to the detection limit. The utility model discloses in, realize the automatic dilution of sample liquid gradient through setting up 2 dilution ponds, improve the dilution accuracy, reduce the dilution error. The utility model relates to a specific embodiment, the dilution pond is respectively for diluting pond I6-1, diluting pond II 6-2, and the transfer of appearance liquid between stoste pond 5, diluting pond I6-1, diluting pond II 6-2 is realized through first rotatory liquid-transfering platform 20, as shown in fig. 5.
In the utility model, the stock solution pool 5 is connected with a purified water pipeline, a waste liquid discharge pipeline and a sample introduction pipeline; and the diluting tank is provided with a purified water pipeline, a waste liquid discharge pipeline, a high-pressure gas pipeline and a pipeline connected with the ion detection assembly.
The utility model discloses an in the embodiment, dilution pond I6-1, dilution pond II 6-2 are the gradient dilution pond, still are connected with constant volume pipeline 18 on it, and dilution pond I6-1, dilution pond II 6-2 are 10-50 times, 100 one times respectively to the dilution multiple of liquid appearance. In one embodiment, the dilution is 10-fold and 100-fold, respectively, and the dilution operation is as follows: 18mL of purified water, namely the dilution pool I6-1, is fed through a purified water pipeline 11, 2mL of liquid sample is taken from the stock solution pool 5 by using a first rotary pipetting platform 20 gun head and is put into the dilution pool I6-1, and a magnetic stirrer 37-1 is used for uniformly mixing; purified water is added in the same method, 2mL of liquid sample is taken from the dilution pool I6-1 and is put into the dilution pool II 6-2, and the magnetic stirrer 37-2 is used for mixing uniformly.
The utility model discloses in, the purified water is pure water, sterile water, deionized water, two distilled water or ultrapure water, chooses for use according to the experiment needs. In addition, in a specific experiment, a diluting solvent may be used instead of purified water, and other solvents for dilution may be used as long as the experimental conditions permit. The utility model adopts purified water. The consumption of the purified water in the dilution pool I6-1 and the dilution pool II 6-2 is controlled by the combined action of the purified water pipeline power source and the pipeline control valve, as shown in fig. 3 and 4. In the diluting process, purified water fed by a purified water pipeline is used as a diluting solvent, and after dilution is completed, the purified water is used for cleaning the pipeline, a stock solution pool and a diluting pool.
Specifically, the purified water inlet pipes in the raw liquid tank 5 and the dilution tank 6 in fig. 3 are independent pipes, and the amount of purified water is controlled by an independent power source and a pipe control valve.
In another specific embodiment, as shown in fig. 4, the diluting pool I6-1 and the diluting pool II 6-2 are diluting pools with constant volume structures, and the diluting pools have constant volume pipes 18. The volume of the diluting tank can be customized according to the size, wherein one mode is the same as the structure and the outer diameter of the diluting tank, and the volume of the diluting tank can be changed by changing the inner diameter; the diluting pool can be customized into different structures and outer diameters according to requirements, so that the inner diameter and the volume of contained liquid are correspondingly changed. In this embodiment, the stock solution tank 5 and the dilution tank 6 share one purified water pipeline, the amount of the purified water used as the diluting solvent in the dilution tank is realized according to the constant volume structure, and when the amount of the purified water entering is larger than the constant volume, the purified water overflows from the constant volume pipeline 18.
The liquid sample is diluted and uniformly mixed by the sample processing module 1, the liquid sample in the diluting pool enters the photoelectric detection component 8 and the ion detection component 10 through the liquid transfer pipeline 27, enters the enzyme membrane detection component 9 through the sampling needle of the second rotary pipetting platform 21, and then is detected and analyzed; the pipelines for the liquid sample to enter the photoelectric detection assembly 8 and the ion detection assembly 10 are provided with electromagnetic valves. The photoelectric detection component 8 comprises a fiber spectrometer and an injection pump 25, wherein a fiber spectrometer detection probe 24 is arranged on the injection pump 25, and the photoelectric detection index of the liquid sample can be detected based on a laser system. The enzyme membrane detection component 9 carries out enzyme membrane detection by arranging an enzyme membrane detection electrode 28 in the detection cell, wherein the enzyme membrane can be one or more of a glucolase membrane, a lactic acid lase membrane, a glutaminase membrane and a lysinase membrane. The ion detection assembly 12 performs ion detection by arranging an ion electrode in the detection cell, wherein the electrode may be one or more of a pH electrode, an ammonia ion electrode, a sodium ion electrode, a potassium ion electrode, and a calcium ion electrode. After finishing liquid sample detection, remaining liquid samples in the stock solution pool 5 and the dilution pool 6 enter the waste liquid discharge pipeline 12 through pipelines, air enters the left end of a main pipeline of the waste liquid discharge pipeline 12, a power source 15-4 is connected to the position, close to the waste liquid bottle 17, of the right end of the main pipeline, and the power source 15-4 is further a self-sucking pump, and a specific embodiment is a peristaltic pump. After liquid samples in the stock solution tank 5 and the dilution tank 6 are discharged, purified water is fed from a purified water pipeline 11 to be used for cleaning the stock solution tank 5, the dilution tank 6 and related pipelines thereof, and after cleaning is finished, high-pressure air is fed through a high-pressure gas pipeline 13 to be used for drying the dilution tank.
More specifically, the dilution pool I6-1 or the dilution pool II 6-2 enters the photoelectric detection assembly 8 through the multi-channel valve 26, as shown in FIG. 6, the multi-channel valve 26 is connected with the photoelectric detection assembly 8, the dilution pool I6-1, the dilution pool II 6-2, the waste liquid discharge pipeline 12 and the enzyme membrane detection assembly 9, and in addition, a valve communicated with the atmosphere is further arranged on the multi-channel valve 26.
More specifically, during photoelectric detection, the multi-channel valve 26 is rotated to communicate the standard liquid tank 19 with the photoelectric detection assembly 8, the injection pump 25 sucks standard liquid from the standard liquid tank 19, the standard liquid is rinsed and then calibrated, and the standard liquid is pushed into the waste liquid discharge pipeline 12 by the injection pump 25 after calibration is completed; then sample liquid is absorbed from the dilution pool I6-1 or the dilution pool II 6-2 for rinsing and then photoelectric detection is carried out.
Further specifically, during enzyme membrane detection, the enzyme membrane reaction tank of the enzyme membrane detection assembly is connected with a buffer solution pipeline, a waste liquid discharge pipeline and a constant volume pipeline, and the standard solution and the diluted solution sample are subjected to liquid transfer and sample introduction through a second rotary liquid transfer platform 21. When the enzyme membrane reaction tank is in a non-working state, the buffer solution is filled in the enzyme membrane reaction tank 30, the buffer solution is discharged through a waste liquid discharge pipe at the bottom of the buffer solution, the enzyme membrane is activated by the buffer solution when the enzyme membrane reaction tank works, the sample volume of the buffer solution reaches the position of a constant volume pipeline, if the buffer solution is higher than the constant volume pipeline, the surplus buffer solution overflows from the constant volume pipeline 18-3, then the standard solution with the constant volume is subjected to enzyme membrane detection calibration, the liquid in the enzyme membrane reaction tank is discharged from the waste liquid discharge pipe at the bottom after the detection is finished, then the liquid sample is obtained, the method is the same as the standard solution and is subjected to detection, after the detection is finished, the buffer solution is fed from the buffer solution bottle 18 to clean the reaction tank, the buffer solution is filled in the reaction tank again after the cleaning is carried out for 3-4 times, and the next liquid sample detection is carried out. An enzyme membrane detection electrode 28 is arranged in the enzyme membrane reaction tank, and the enzyme membrane detection electrode can be one or more of a glucolase membrane, a lactic acid enzyme membrane, a glutamic acid enzyme membrane and a lysine enzyme membrane. The utility model discloses in be provided with the enzyme membrane determine module of two different enzyme membranes, also can set up a plurality of different enzyme membrane determine module as required. The pipelines in the enzyme membrane detection assembly are all provided with electromagnetic valves for controlling the opening and closing of the pipelines, and the buffer solution pipeline is also provided with a peristaltic pump 15-6. In order to make the enzyme membrane reaction proceed under constant temperature, a constant temperature device 38 is arranged under the enzyme membrane detection pool.
Further specifically, the diluting pool I6-1 is further connected with a pipeline communicated to the ion detecting component 10, the ion detecting component 10 includes an ion detecting pool 36 and an electrode 33, a pool body of the ion detecting pool 36 is provided with a sample introduction pipeline 34 for entering a liquid sample, a purified water inlet pipeline 11, a waste liquid discharge pipeline 35, which are a branch of the waste liquid discharge pipeline 12, a constant volume pipeline 18-4, a high pressure gas pipeline 13, and a pipeline for connecting the ion low-standard solvent 31 and the ion high-standard solvent 32, as shown in fig. 8. The ion detection cell 36 is provided with an electrode 33 which is a pH electrode 33-1 (the pH electrode can be used for detecting pH, more importantly, the pH electrode can be used as a reference electrode for other ion detection) and other detection electrodes 33-2, the other detection electrodes 31-2 are one or more of an ammonia ion electrode, a sodium ion electrode, a potassium ion electrode and a calcium ion electrode, the ion detection cell 36 is divided into a plurality of small detection cells which are communicated, the number of the small detection cells is two or more, the specific number is not limited, the electrodes are arranged in the small detection cells, and the structure effectively saves detection sample liquid and reagents.
Before ion detection, the ion detection cell 36 does not contain any liquid, and the detection process is as follows: firstly, cleaning, namely feeding purified water through a purified water pipeline 11 for cleaning, and then feeding high-pressure gas through a high-pressure gas pipeline 13 for drying the ion detection cell; secondly, calibration, namely, feeding an ion low-standard solvent 31 for calibrating low standard, discharging waste after the calibration is finished, feeding purified water for cleaning, blowing the ion detection tank by high-pressure gas, feeding an ion high-standard solvent 32 for calibrating high standard, discharging waste after the calibration is finished, feeding purified water for cleaning, and blowing the ion detection tank by high-pressure gas; detecting the liquid sample again, and detecting the liquid in the dilution pool I6-1; and after the detection is finished, discharging waste, feeding purified water for cleaning, and blowing the ion detection pool by high-pressure gas for next detection. In the ion detection process, the opening and closing of the pipelines are controlled by respective pipeline control valves, and liquid sample power is provided according to the corresponding power source setting.
In a specific embodiment, a constant temperature module is arranged below the enzyme membrane reaction tank to ensure that the enzyme membrane reaction is carried out at a constant temperature; the ion detection cell 36 is provided with a temperature sensor 34, which is used for temperature compensation during software calculation. The utility model discloses in, the power supply is the peristaltic pump in a specific embodiment, and the pipeline control valve is the solenoid valve.
In the utility model, the pipeline is a silicone tube, a polytetrafluoroethylene tube and/or an organic plastic hose. In a specific embodiment, the pipeline for conveying the liquid sample is a polytetrafluoroethylene pipe, the material has good hydrophobicity, and the pipeline made of the material can effectively reduce waste caused by wall hanging of the liquid sample and increase sampling accuracy; common silicone tubes or organic plastic hoses are adopted for other pipelines.
In one embodiment, the inner diameter of the tube is 0.1 to 15 mm; further preferably 0.5 to 5 mm; the inner diameters of the pipelines can be completely consistent or not, and can be set to be reasonable according to requirements, and the inner diameter range can be within a set value.
In an embodiment, the utility model discloses sample processing module, photoelectric detection subassembly, enzyme membrane determine module, ion detection subassembly, control system, display interface set up in a box, as shown in fig. 2, be equipped with power source, data interface, USB interface, heat dissipation window, solenoid valve, peristaltic pump, pipeline interface on the box wall. In the box body, the rotating diameter of the first rotating track 22 is 15-18cm, and the rotating diameter of the second rotating track 23 is 20-23 cm. The utility model discloses in, full-automatic biological on-line measuring device size length wide is (800 cake 900) mm (450 cake 550) mm (600 cake 700) mm, further is 860mm 520mm 670mm, and its inner structure is inseparable, effectively practices thrift equipment space.
In order to realize detecting automated control, the utility model discloses still including the control module who is used for controlling the appearance of advancing, it is through moving liquid platform, photoelectric detection subassembly, enzyme membrane determine module, ion detect subassembly to stoste pond, dilution pond I, dilution pond II, sample to and pipeline power supply, pipeline control valve link to each other with total control system, realize sample treatment and detecting system's automated control. The control module comprises a general processor, a digital signal processor, an Application Specific Integrated Circuit (ASIC) or a Field Programmable Gate Array (FPGA), and can be used for programming the control unit according to the sampling requirement. The control unit receives an externally input dilution signal, which comprises sample liquid dilution multiple, photoelectric detection parameters, ion detection parameters and enzyme membrane detection parameters, the control unit outputs a control signal to control a power source and a pipeline control valve, so that the liquid sample is diluted reasonably by multiple, the photoelectric detection is controlled to be detected in a whole wave band of 350-plus 800nm, the enzyme membrane detection indexes are controlled to be glucose 0.1-200 g/L, xylose 0.1-200 g/L, lactic acid 0.1-200 g/L, lysine 0.1-200 g/L, glutamic acid 0.1-14 g/L, ethanol 0.1-200 g/L, the ion detection indexes are controlled to be detected in a range of 0-14, ammonia nitrogen 0.1-3000 mg/L, sodium ions 0.5-3000 mg/L, potassium ions 0.5-3000 mg/L and calcium ions 0.2-18000 mg/L, and analyzing and storing the data.
Be convenient for operating personnel right the utility model discloses the operation control of device, sample treatment and detecting system still includes display interface 4, like fig. 2, display interface can show the utility model discloses the working condition of system, for example: and displaying the dilution times, the detection parameters and the detection results, the states of the cleaning and disinfection work and the like. For convenient operation, the display interface is a display screen, and further is a touchable display screen, and display operation buttons are arranged on the display interface to realize control over the control unit.
The utility model integrates the liquid receiving and transferring platform, the enzyme membrane detection component, the photoelectric detection component and the ion detection component, and is controlled by the control system, thereby realizing the innovation of the full-automatic biological online detection device; the utility model discloses the structure is inseparable, especially moves the liquid platform and has increased the space utilization, makes the device volume reduce greatly.
The utility model effectively reduces the dilution error by performing gradient dilution through the liquid transfer platform, which is helpful to improve the detection accuracy; the diluting pool is connected with a pipeline for conveying high-pressure gas, the liquid sample is diluted and cleaned by purified water, and the dilution error of the next sample is reduced through the high-pressure gas drying pipeline.
Claims (10)
1. A full-automatic biological online detection device is characterized by comprising a sample processing module, a detection and analysis module, a control module and a display interface, wherein the sample processing module comprises an operation bin, a stock solution pool, a dilution pool and a liquid transfer platform, the stock solution pool and the dilution pool are arranged at the bottom of the operation bin, and the detection and analysis module comprises an enzyme membrane detection assembly arranged in the operation bin, a photoelectric detection assembly and an ion detection assembly outside the operation bin; the control module is connected with the liquid transfer platform, the enzyme membrane detection assembly, the photoelectric detection assembly and the ion detection assembly; the display interface is connected with the control module and used for displaying the pipetting action state, the liquid sample dilution multiple, the real-time detection data and the data analysis curve.
2. The automatic on-line biological detection device of claim 1, wherein the pipetting platform comprises a first rotary pipetting platform and a second rotary pipetting platform, and the pipetting channels thereof are rotationally positioned and pipetted along with the first rotary pipetting platform and the second rotary pipetting platform.
3. The automatic biological on-line detection device of claim 2, wherein the first rotary pipetting platform and the second rotary pipetting platform are both in rotary motion along the vertical axis direction and in up-and-down motion along the vertical axis direction.
4. The automatic biological online detection device according to claim 3, wherein the sample transfer ports of the first rotary pipetting platform and the second rotary pipetting platform are provided with 1mL, 5mL or 10mL tips or sampling needles.
5. The automatic biological on-line detection device of claim 3, wherein a dilution pool is arranged at the intersection of the first rotary pipetting platform and the second rotary pipetting platform, a stock solution pool is further arranged on the first rotary pipetting platform, and an enzyme membrane reaction pool and a standard solution pool are further arranged on the second rotary pipetting platform.
6. The automatic biological online detection device of claim 5, wherein the stock solution tank, the dilution tank, the enzyme membrane reaction tank and the standard solution tank are all embedded into the bottom of the operation bin, and the two dilution tanks are constant-volume dilution tanks.
7. The automatic on-line biological detection device as claimed in claim 6, wherein the dilution tank is provided with a high pressure air pipeline, and the bottom of the dilution tank is provided with a magnetic stirrer.
8. The automatic on-line biological detection device as claimed in claim 1, wherein the photoelectric detection assembly comprises a fiber spectrometer and a syringe pump, and the detection probe of the fiber spectrometer is arranged on the syringe pump.
9. The automatic biological on-line detection device of claim 1, wherein the enzyme membrane detection component is one or more of a glucose enzyme membrane, a lactate enzyme membrane, a glutamate enzyme membrane and a lysine enzyme membrane component.
10. The automatic biological online detection device of claim 1, wherein the ion detection component is one or more of a pH electrode, an ammonia ion electrode, a sodium ion electrode, a potassium ion electrode and a calcium ion electrode component.
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| CN202022683897.6U CN214473430U (en) | 2020-11-18 | 2020-11-18 | Full-automatic biological on-line measuring device |
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| CN202022683897.6U CN214473430U (en) | 2020-11-18 | 2020-11-18 | Full-automatic biological on-line measuring device |
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