CN116998635A - Feed additive for penaeus vannamei boone cultivation and preparation method and application thereof - Google Patents
Feed additive for penaeus vannamei boone cultivation and preparation method and application thereof Download PDFInfo
- Publication number
- CN116998635A CN116998635A CN202311088895.4A CN202311088895A CN116998635A CN 116998635 A CN116998635 A CN 116998635A CN 202311088895 A CN202311088895 A CN 202311088895A CN 116998635 A CN116998635 A CN 116998635A
- Authority
- CN
- China
- Prior art keywords
- vibrio
- parts
- inhibitor
- penaeus vannamei
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 106
- 241000238553 Litopenaeus vannamei Species 0.000 title claims abstract description 95
- 239000003674 animal food additive Substances 0.000 title claims abstract description 66
- 238000012364 cultivation method Methods 0.000 title description 2
- 238000000855 fermentation Methods 0.000 claims abstract description 122
- 230000004151 fermentation Effects 0.000 claims abstract description 117
- 241000607598 Vibrio Species 0.000 claims abstract description 110
- 241000001727 Tropicoporus linteus Species 0.000 claims abstract description 77
- 241000193171 Clostridium butyricum Species 0.000 claims abstract description 64
- 239000003112 inhibitor Substances 0.000 claims abstract description 60
- -1 amino beta-cyclodextrin Chemical compound 0.000 claims abstract description 41
- 238000002156 mixing Methods 0.000 claims abstract description 36
- 239000003094 microcapsule Substances 0.000 claims abstract description 32
- 229920000858 Cyclodextrin Polymers 0.000 claims abstract description 25
- 239000001116 FEMA 4028 Substances 0.000 claims abstract description 25
- 235000011175 beta-cyclodextrine Nutrition 0.000 claims abstract description 25
- 229960004853 betadex Drugs 0.000 claims abstract description 25
- 239000012752 auxiliary agent Substances 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
- MWOOGOJBHIARFG-UHFFFAOYSA-N vanillin Chemical compound COC1=CC(C=O)=CC=C1O MWOOGOJBHIARFG-UHFFFAOYSA-N 0.000 claims abstract description 23
- FGQOOHJZONJGDT-UHFFFAOYSA-N vanillin Natural products COC1=CC(O)=CC(C=O)=C1 FGQOOHJZONJGDT-UHFFFAOYSA-N 0.000 claims abstract description 23
- 235000011347 Moringa oleifera Nutrition 0.000 claims abstract description 22
- 235000012141 vanillin Nutrition 0.000 claims abstract description 22
- 239000000341 volatile oil Substances 0.000 claims abstract description 19
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims abstract description 15
- 239000004359 castor oil Substances 0.000 claims abstract description 15
- 235000019438 castor oil Nutrition 0.000 claims abstract description 15
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 claims abstract description 15
- 239000008367 deionised water Substances 0.000 claims abstract description 14
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 14
- 239000002994 raw material Substances 0.000 claims abstract description 11
- 239000001963 growth medium Substances 0.000 claims description 25
- 239000007788 liquid Substances 0.000 claims description 25
- 238000009630 liquid culture Methods 0.000 claims description 25
- 239000000463 material Substances 0.000 claims description 19
- 241000125205 Anethum Species 0.000 claims description 18
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 claims description 18
- 239000000843 powder Substances 0.000 claims description 14
- 239000000203 mixture Substances 0.000 claims description 12
- 239000011162 core material Substances 0.000 claims description 11
- 238000004108 freeze drying Methods 0.000 claims description 11
- 229920002085 Dialdehyde starch Polymers 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 10
- 238000009210 therapy by ultrasound Methods 0.000 claims description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 7
- 239000002245 particle Substances 0.000 claims description 7
- 230000002829 reductive effect Effects 0.000 claims description 7
- 239000008103 glucose Substances 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 240000000249 Morus alba Species 0.000 claims description 5
- 235000008708 Morus alba Nutrition 0.000 claims description 5
- 239000001888 Peptone Substances 0.000 claims description 5
- 108010080698 Peptones Proteins 0.000 claims description 5
- 244000061456 Solanum tuberosum Species 0.000 claims description 5
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 235000019319 peptone Nutrition 0.000 claims description 5
- 238000003825 pressing Methods 0.000 claims description 5
- 238000010298 pulverizing process Methods 0.000 claims description 5
- 238000001694 spray drying Methods 0.000 claims description 5
- 239000007858 starting material Substances 0.000 claims description 4
- 241000220215 Moringa Species 0.000 claims 4
- 230000000694 effects Effects 0.000 abstract description 22
- 244000179886 Moringa oleifera Species 0.000 abstract description 18
- 230000001717 pathogenic effect Effects 0.000 abstract description 18
- 230000004083 survival effect Effects 0.000 abstract description 9
- 235000013305 food Nutrition 0.000 abstract description 7
- 240000000662 Anethum graveolens Species 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 13
- 241000238557 Decapoda Species 0.000 description 12
- 241000607272 Vibrio parahaemolyticus Species 0.000 description 12
- 238000012360 testing method Methods 0.000 description 12
- 230000001976 improved effect Effects 0.000 description 11
- 206010047400 Vibrio infections Diseases 0.000 description 7
- 239000004480 active ingredient Substances 0.000 description 7
- 210000001035 gastrointestinal tract Anatomy 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 6
- 241000607626 Vibrio cholerae Species 0.000 description 5
- 210000000170 cell membrane Anatomy 0.000 description 5
- 235000001727 glucose Nutrition 0.000 description 5
- 150000004676 glycans Chemical class 0.000 description 5
- 230000036039 immunity Effects 0.000 description 5
- 230000002401 inhibitory effect Effects 0.000 description 5
- 230000005764 inhibitory process Effects 0.000 description 5
- 230000000968 intestinal effect Effects 0.000 description 5
- 229920001282 polysaccharide Polymers 0.000 description 5
- 239000005017 polysaccharide Substances 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 229940118696 vibrio cholerae Drugs 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 230000000844 anti-bacterial effect Effects 0.000 description 4
- 244000052616 bacterial pathogen Species 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 4
- 230000006870 function Effects 0.000 description 4
- 230000003834 intracellular effect Effects 0.000 description 4
- 150000007524 organic acids Chemical class 0.000 description 4
- 230000035699 permeability Effects 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000168517 Haematococcus lacustris Species 0.000 description 3
- 150000004753 Schiff bases Chemical group 0.000 description 3
- 241000607594 Vibrio alginolyticus Species 0.000 description 3
- 241000607618 Vibrio harveyi Species 0.000 description 3
- 241000607265 Vibrio vulnificus Species 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 230000006907 apoptotic process Effects 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 230000005584 early death Effects 0.000 description 3
- 230000001939 inductive effect Effects 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- 239000003223 protective agent Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000002195 synergetic effect Effects 0.000 description 3
- 238000009423 ventilation Methods 0.000 description 3
- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 description 2
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 2
- 241000414067 Inonotus obliquus Species 0.000 description 2
- 241000927735 Penaeus Species 0.000 description 2
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- UYXTWWCETRIEDR-UHFFFAOYSA-N Tributyrin Chemical compound CCCC(=O)OCC(OC(=O)CCC)COC(=O)CCC UYXTWWCETRIEDR-UHFFFAOYSA-N 0.000 description 2
- 108010046334 Urease Proteins 0.000 description 2
- 235000013793 astaxanthin Nutrition 0.000 description 2
- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 description 2
- 229940022405 astaxanthin Drugs 0.000 description 2
- 239000001168 astaxanthin Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- ULDHMXUKGWMISQ-UHFFFAOYSA-N carvone Chemical compound CC(=C)C1CC=C(C)C(=O)C1 ULDHMXUKGWMISQ-UHFFFAOYSA-N 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000004132 cross linking Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 239000003205 fragrance Substances 0.000 description 2
- 210000000514 hepatopancreas Anatomy 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 230000009545 invasion Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- XMGQYMWWDOXHJM-UHFFFAOYSA-N limonene Chemical compound CC(=C)C1CCC(C)=CC1 XMGQYMWWDOXHJM-UHFFFAOYSA-N 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 208000011580 syndromic disease Diseases 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- OGLDWXZKYODSOB-UHFFFAOYSA-N α-phellandrene Chemical compound CC(C)C1CC=C(C)C=C1 OGLDWXZKYODSOB-UHFFFAOYSA-N 0.000 description 2
- LFJQCDVYDGGFCH-JTQLQIEISA-N (+)-β-phellandrene Chemical compound CC(C)[C@@H]1CCC(=C)C=C1 LFJQCDVYDGGFCH-JTQLQIEISA-N 0.000 description 1
- LFJQCDVYDGGFCH-SNVBAGLBSA-N (+/-)-beta-Phellandrene Natural products CC(C)[C@H]1CCC(=C)C=C1 LFJQCDVYDGGFCH-SNVBAGLBSA-N 0.000 description 1
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- ZPSJGADGUYYRKE-UHFFFAOYSA-N 2H-pyran-2-one Chemical class O=C1C=CC=CO1 ZPSJGADGUYYRKE-UHFFFAOYSA-N 0.000 description 1
- VJLLDPFYQAJLKH-UHFFFAOYSA-N 3-(2-phenylethenyl)pyran-2-one Chemical group O=C1OC=CC=C1C=CC1=CC=CC=C1 VJLLDPFYQAJLKH-UHFFFAOYSA-N 0.000 description 1
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- 241000132012 Atractylodes Species 0.000 description 1
- 206010003694 Atrophy Diseases 0.000 description 1
- 241000202726 Bupleurum Species 0.000 description 1
- 239000005973 Carvone Substances 0.000 description 1
- 241000238424 Crustacea Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 208000004232 Enteritis Diseases 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 208000007882 Gastritis Diseases 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 244000303040 Glycyrrhiza glabra Species 0.000 description 1
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 description 1
- 108010006464 Hemolysin Proteins Proteins 0.000 description 1
- 206010028851 Necrosis Diseases 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- BMZNDTYXXZLYHM-UHFFFAOYSA-N OC1CC2=CC=CC=C2OC1C1=CC=CC=C1.O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 Chemical compound OC1CC2=CC=CC=C2OC1C1=CC=CC=C1.O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 BMZNDTYXXZLYHM-UHFFFAOYSA-N 0.000 description 1
- 244000236658 Paeonia lactiflora Species 0.000 description 1
- 235000008598 Paeonia lactiflora Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 241001180876 Saposhnikovia Species 0.000 description 1
- 239000002262 Schiff base Substances 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 244000290333 Vanilla fragrans Species 0.000 description 1
- 235000009499 Vanilla fragrans Nutrition 0.000 description 1
- 235000012036 Vanilla tahitensis Nutrition 0.000 description 1
- 241000544286 Vibrio anguillarum Species 0.000 description 1
- 241000607253 Vibrio mimicus Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- OGLDWXZKYODSOB-SNVBAGLBSA-N alpha-phellandrene Natural products CC(C)[C@H]1CC=C(C)C=C1 OGLDWXZKYODSOB-SNVBAGLBSA-N 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000001775 anti-pathogenic effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000037444 atrophy Effects 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- LFJQCDVYDGGFCH-UHFFFAOYSA-N beta-phellandrene Natural products CC(C)C1CCC(=C)C=C1 LFJQCDVYDGGFCH-UHFFFAOYSA-N 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 210000005056 cell body Anatomy 0.000 description 1
- 238000010382 chemical cross-linking Methods 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000013270 controlled release Methods 0.000 description 1
- ZYGHJZDHTFUPRJ-UHFFFAOYSA-N coumarin Chemical group C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229930004069 diterpene Natural products 0.000 description 1
- 150000004141 diterpene derivatives Chemical class 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 231100000249 enterotoxic Toxicity 0.000 description 1
- 230000002242 enterotoxic effect Effects 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- QOLIPNRNLBQTAU-UHFFFAOYSA-N flavan Chemical compound C1CC2=CC=CC=C2OC1C1=CC=CC=C1 QOLIPNRNLBQTAU-UHFFFAOYSA-N 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 230000007760 free radical scavenging Effects 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 239000003228 hemolysin Substances 0.000 description 1
- 208000008025 hordeolum Diseases 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000004957 immunoregulator effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000007413 intestinal health Effects 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 229930013686 lignan Natural products 0.000 description 1
- 235000009408 lignans Nutrition 0.000 description 1
- 150000005692 lignans Chemical class 0.000 description 1
- 229940087305 limonene Drugs 0.000 description 1
- 235000001510 limonene Nutrition 0.000 description 1
- 230000003859 lipid peroxidation Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 235000011477 liquorice Nutrition 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000007102 metabolic function Effects 0.000 description 1
- 239000004530 micro-emulsion Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000003950 pathogenic mechanism Effects 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- 239000011241 protective layer Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229940107700 pyruvic acid Drugs 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 229930004725 sesquiterpene Natural products 0.000 description 1
- 150000004354 sesquiterpene derivatives Chemical class 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 1
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 1
- 238000013112 stability test Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 150000003648 triterpenes Chemical class 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/11—Aldehydes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7028—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages
- A61K31/7034—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/145—Clostridium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Abstract
The invention relates to the technical field of feed additives, in particular to a feed additive for penaeus vannamei boone cultivation and a preparation method and application thereof, and the feed additive comprises the following raw materials in parts by mass: 3-7 parts of a first vibrio inhibitor, 1-3 parts of a second vibrio inhibitor, 5-7 parts of total amino beta-cyclodextrin, 1-2 parts of a microcapsule auxiliary agent, 0.3-0.5 part of polyoxyethylene 40 hydrogenated castor oil, 10-15 parts of a mixed fermentation preparation and 50-70 parts of deionized water; the first vibrio inhibitor is prepared by mixing moringa seed extract and vanillin according to a mass ratio of 1 (0.2-0.5); the second vibrio inhibitor is dill essential oil; the mixed fermentation preparation is prepared by mixing clostridium butyricum fermentation preparation and Phellinus linteus fermentation preparation according to the mass ratio of 1 (0.15-0.3). The feed additive prepared by the invention can effectively inhibit pathogenic vibrio in water, improve the survival rate of penaeus vannamei boone and has the effects of food calling and aroma enhancement.
Description
Technical Field
The invention relates to the technical field of feed additives, and also relates to the technical field of microbial fermentation, in particular to a feed additive for penaeus vannamei boone cultivation, a preparation method and application thereof.
Background
The penaeus vannamei is aquatic crustacean of the genus penaeus of the family of the order of the decapetales, the family of the penaeus, the academic name of the penaeus vannamei, also called white-skin penaeus vannamei, base penaeus vannamei, and penaeus vannamei, has light grey body color, fine spots on the body surface, thin shell, rich nutrition and delicious taste, and becomes one of three main penaeus vannamei breeds in the world. In the cultivation process of the penaeus vannamei boone, the penaeus vannamei boone is easy to be infected by vibrio pathogenic bacteria, the penaeus vannamei boone is induced to suffer from vibriosis, and the vibriosis of the penaeus vannamei boone can rapidly spread in a short time, so that the penaeus vannamei boone dies in a large scale. Vibrio pathogens include Vibrio cholerae, vibrio parahaemolyticus, vibrio vulnificus, vibrio alginolyticus, vibrio harveyi, vibrio mimicus, vibrio anguillarum, etc., which can cause vibriosis such as early death syndrome (EMS) of prawns, acute necrosis of the liver and pancreas of prawns (AHPND), etc. For example, vibrio parahaemolyticus is a main pathogenic bacterium inducing early death syndrome of penaeus vannamei, and the body color of diseased penaeus vannamei is white and reddish, and is accompanied by hepatopancreas swelling, soft texture and obvious atrophy of the hepatopancreas of partial diseased penaeus vannamei. The pathogenic mechanism of vibrio parahaemolyticus is: 1) Vibrio parahaemolyticus produces a haemolytoxin: the hemolysin can cause the cells in the penaeus vannamei boone to be hemolyzed, so that the penaeus vannamei boone dies; 2) Vibrio parahaemolyticus has enterotoxin effect: the digestive tract tissues of the penaeus vannamei are damaged, so that the ileal erosion, the gastric mucositis and the digestive dysfunction are caused; 3) Vibrio parahaemolyticus produces urease: urease can accumulate intestinal juice of Penaeus vannamei Boone, cause intestinal water accumulation, and can cause the problem of pulling Bai Bian when serious; 4) The extracellular secretion of vibrio parahaemolyticus can reduce the immunity of the penaeus vannamei boone, cause growth and development retardation, and easily cause other diseases.
Therefore, the novel feed additive for preventing and controlling pathogenic vibrios is always a research hot spot in the field of penaeus vannamei boone cultivation. For example, the patent of the invention with publication number of CN103283990B discloses application of inonotus obliquus polysaccharide as a feed additive for litopenaeus vannamei, wherein the addition amount of inonotus obliquus polysaccharide in the litopenaeus vannamei feed is 500-1500mg/kg, thereby effectively improving the growth performance, immunity and oxidation resistance of the litopenaeus vannamei, providing a new way for developing the litopenaeus vannamei feed and promoting the sustainable development of the litopenaeus vannamei breeding industry. The invention patent with publication number of CN107494998B discloses a composite feed additive for inhibiting vibrio in intestinal tracts of penaeus vannamei, which comprises the following components in percentage by weight: citric acid 8-12%, clostridium butyricum 10 12 cfu/kg～5×10 12 cfu/kg, 5-8% of bupleurum, 3-6% of bighead atractylodes rhizome, 2-5% of white peony root, 2-5% of divaricate saposhnikovia root, 1-3% of dried orange peel and 1-3% of liquorice, can effectively and selectively reduce the number of vibrios in the intestinal tracts of the prawns, can effectively avoid the damage effect of clostridium butyricum on the liver, and can improve the capability of the prawns in defending exogenous vibrios from invading the intestinal tracts, thereby effectively preventing the vibriosis of the prawnsRaw and its induced death. For example, the invention patent with publication number of CN112369534B discloses a feed additive for preventing early death of prawns, a preparation method and application thereof, firstly, wall-broken haematococcus pluvialis is coated once to isolate haematococcus pluvialis from air to avoid oxidization, and then the wall-broken haematococcus pluvialis algae liquid coated once is mixed with silicon dioxide adsorbed with organic compound acid and tributyrin to obtain mixed particles, and the mixed particles are coated twice to wrap organic acid active ingredients; the relative independence of the additive is improved through a double-layer coating process, the reaction of active ingredients and feed ingredients in the application process is effectively avoided, the loss of organic acid caused by long-time soaking of the prawn feed in a water body is avoided, the defects of high temperature tolerance and easy oxidation in the astaxanthin processing process are overcome to a certain extent, the stable and effective absorption of the astaxanthin is ensured, the synergistic effect with the organic acid ingredients is exerted, the proliferation of pathogenic vibrio is inhibited, the intestinal flora balance of the prawn is improved, and the organism immunity and disease resistance of the prawn are enhanced. The invention patent with publication number CN114672446B discloses a preparation method and application of clostridium butyricum preparation, which comprises the following steps: activating strains to obtain clostridium butyricum fermentation liquor; centrifuging clostridium butyricum fermentation liquor, collecting bacterial mud, adding a protective agent solution, uniformly mixing, adding a wall material solution, uniformly mixing, and finally freeze-drying to obtain clostridium butyricum preparation; wherein the protective agent is a mixture of skim milk, beta-cyclodextrin, glucose and dipotassium hydrogen phosphate; the wall material is gelatin, sodium alginate or sodium carboxymethylcellulose; through the dual functions of the protective agent and the wall material, the field planting of clostridium butyricum in the intestinal tracts of the litopenaeus vannamei can be effectively ensured, and further, the repair of the intestinal health of the litopenaeus vannamei can be ensured.
However, the existing feed additives generally use coating materials or wall materials to physically wrap active contents, such as CN112369534B, CN114672446B, and as chemical crosslinking action does not occur among molecules of the coating materials, molecular gaps are larger, the outer wall of the formed microcapsule feed additive is loose and not compact enough, the wrapping stability of the microcapsule feed additive needs to be further improved, the volatile active ingredients still have the possibility of overflowing and losing, and further the effect of the feed additive on inhibiting vibrio pathogenic bacteria and feeding is affected, in particular, after the feed additive encounters water or enters a culture water body, the dissolution and release speed of the feed additive is still higher, and the effect of long-acting slow-release and controlled release is difficult to achieve.
Disclosure of Invention
Therefore, the invention aims to provide a feed additive for penaeus vannamei boone cultivation and a preparation method and application thereof, on one hand, a novel feed additive is developed to further improve the effect of preventing and treating vibrio vannamei boone, and on the other hand, the inclusion stability of the microcapsule feed additive is improved to prevent the loss of volatile active components, prolong the slow-release time and keep good antibacterial phagostimulant effect.
Based on the purposes, the invention provides a feed additive for penaeus vannamei boone cultivation, which comprises the following raw materials in parts by mass: 3-7 parts of a first vibrio inhibitor, 1-3 parts of a second vibrio inhibitor, 5-7 parts of total amino beta-cyclodextrin, 1-2 parts of a microcapsule auxiliary agent, 0.3-0.5 part of polyoxyethylene 40 hydrogenated castor oil, 10-15 parts of a mixed fermentation preparation and 50-70 parts of deionized water;
the first vibrio inhibitor is prepared by mixing moringa seed extract and vanillin according to a mass ratio of 1 (0.2-0.5);
the chemical structural formula of the moringa seed element is as follows:
after being treated by moringa oleifera seed extract, the composition has obvious inhibition effect on pathogenic vibrio of penaeus vannamei boone, vibrio parahaemolyticus, vibrio vulnificus, vibrio alginolyticus, vibrio harveyi and the like. Specifically, the moringa seed element obviously inhibits the growth of pathogenic vibrio of the penaeus vannamei, so that the permeability and damage rate of cell membranes are obviously increased, and intracellular macromolecular nucleic acid, protein and carbohydrate are leaked out of cells; after the moringa seed element is used for treating pathogenic vibrio of the penaeus vannamei, the formation of a vibrio biological film is inhibited, the adhesiveness of the biological film is reduced, and the hydrophobic effect and the swimming mobility of vibrio cells of the vibrio are interfered; spicy foodThe lignan can obviously increase the intracellular Ca of vibrio 2+ Content of Ca to 2+ Unbalanced, the metabolic function of vibrio is damaged, and then the apoptosis of the vibrio is caused; the moringa seed extract can induce and promote the apoptosis process, and the apoptosis rate is gradually increased along with the increase of the dosage of the moringa seed extract;
the vanillin has the chemical structural formula as follows:
vanillin is also called vanillin, has a chemical name of 3-methoxy-4-hydroxybenzaldehyde, has intense vanilla bean aroma and milk aroma, has the functions of enhancing aroma, inducing food and flavoring, improves the feeding rate and the utilization rate of the penaeus vannamei to the feed, meanwhile, the pathogenic vibrio of the penaeus vannamei is used as gram negative bacteria, has loose cell wall structure, is easy to combine with vanillin, changes the intracellular environment by destroying the integrity and permeability of cell membranes, further inhibits the activity of enzymes (for example, the vanillin can inhibit the activity of DNA polymerase), influences the metabolism in the cell body, and prevents the synthesis and expression of genetic materials in the delay period of the pathogenic vibrio of the penaeus vannamei;
the second vibrio inhibitor is dill essential oil, the dill essential oil comprises active ingredients such as carvone, alpha-phellandrene, beta-phellandrene, limonene and the like, pathogenic vibrio of the penaeus vannamei, such as vibrio cholerae, vibrio parahaemolyticus, vibrio vulnificus, vibrio alginolyticus, vibrio harveyi and the like, can effectively kill the pathogenic vibrio of the penaeus vannamei, and the dill essential oil also changes permeability by causing distortion and damage rupture of cell membranes, so that cell contents leak, thereby achieving the antibacterial effect, simultaneously having the free radical removal performance and lipid peroxidation resistance in feed, and being beneficial to improving the immunity of the penaeus vannamei, and further improving the survival rate of the penaeus vannamei; in addition, the dill essential oil can promote the rapid healing of the wound of the penaeus vannamei boone, and reduce the infection risk;
finally, the moringa seed extract, the vanillin and the dill essential oil have the effect of synergistically inhibiting pathogenic vibrio of the penaeus vannamei, the three have complementary enhancement effects on a vibrio inhibition mechanism, multiple vibrio resistance effects are achieved through different approaches and different targets, the integrity and the permeability of a cell membrane are destroyed through the lipid interaction with the cell membrane of the vibrio, the normal biological metabolism of the vibrio is interfered, the growth and the reproduction of the vibrio are inhibited, the formation and the maintenance of a biological membrane are interfered, and the protective layer of the vibrio is destroyed, so that the synergistic effect is achieved;
the mixed fermentation preparation is formed by mixing clostridium butyricum fermentation preparation and Phellinus linteus fermentation preparation according to the mass ratio of 1 (0.15-0.3); through scientific and reasonable combination of clostridium butyricum fermentation preparation and Phellinus linteus fermentation preparation, the intestinal environment of the penaeus vannamei boone is improved, the inhibition effect on pathogenic vibrio is further improved, and the survival rate of the penaeus vannamei boone is further improved.
Further, the feed additive for penaeus vannamei boone cultivation comprises the following raw materials in parts by mass: 3 parts of a first vibrio inhibitor, 1 part of a second vibrio inhibitor, 5 parts of total amino beta-cyclodextrin, 1 part of a microcapsule auxiliary agent, 0.3 part of polyoxyethylene 40 hydrogenated castor oil, 10 parts of a mixed fermentation preparation and 50 parts of deionized water;
the first vibrio inhibitor is formed by mixing moringa seed extract and vanillin according to the mass ratio of 1:0.2;
the second vibrio inhibitor is dill essential oil;
the mixed fermentation preparation is prepared by mixing clostridium butyricum fermentation preparation and Phellinus linteus fermentation preparation according to the mass ratio of 1:0.15.
Further, the feed additive for penaeus vannamei boone cultivation comprises the following raw materials in parts by mass: 5 parts of a first vibrio inhibitor, 2 parts of a second vibrio inhibitor, 6 parts of total amino beta-cyclodextrin, 1.5 parts of a microcapsule auxiliary agent, 0.4 part of polyoxyethylene 40 hydrogenated castor oil, 12.5 parts of a mixed fermentation preparation and 60 parts of deionized water;
the first vibrio inhibitor is prepared by mixing moringa seed extract and vanillin according to a mass ratio of 1:0.35;
the second vibrio inhibitor is dill essential oil;
the mixed fermentation preparation is prepared by mixing clostridium butyricum starter and Phellinus linteus starter according to the mass ratio of 1:0.25.
Further, the feed additive for penaeus vannamei boone cultivation comprises the following raw materials in parts by mass: 7 parts of a first vibrio inhibitor, 3 parts of a second vibrio inhibitor, 7 parts of total amino beta-cyclodextrin, 2 parts of a microcapsule auxiliary agent, 0.5 part of polyoxyethylene 40 hydrogenated castor oil, 15 parts of a mixed fermentation preparation and 70 parts of deionized water;
the first vibrio inhibitor is prepared by mixing moringa seed extract and vanillin according to a mass ratio of 1:0.5;
the second vibrio inhibitor is dill essential oil;
the mixed fermentation preparation is prepared by mixing clostridium butyricum fermentation preparation and Phellinus linteus fermentation preparation according to the mass ratio of 1:0.3.
Further, the microcapsule auxiliary agent is formed by mixing dialdehyde starch and triethylamine according to the mass ratio of 1 (0.1-0.15), dialdehyde starch is used as a cross-linking agent, the dialdehyde starch and the full amino beta-cyclodextrin are mutually cross-linked under the catalysis of the triethylamine, and a Schiff base structure generated by the reaction is used for bridging, so that a compact three-dimensional interpenetrating network structure is formed, an oil-in-water microemulsion system is formed under the action of a polyoxyethylene 40 hydrogenated castor oil O/W type emulsifying agent, a vibrio inhibitor and a mixed fermentation preparation are used as core materials, and the full amino beta-cyclodextrin and the microcapsule auxiliary agent are used as wall materials, so that a microcapsule slow-release structure is formed, on one hand, the invasion of external miscellaneous bacteria is blocked, the pollution of the miscellaneous bacteria is avoided, and on the other hand, the volatilization of volatile aroma components in dill essential oil is avoided, and a good food calling effect of the feed additive is kept for a long time; meanwhile, the full amino beta-cyclodextrin and the dialdehyde starch are combined to serve as wall materials, and Schiff base derivatives generated by the reaction of the full amino beta-cyclodextrin and the dialdehyde starch have antibacterial and bacteriostatic activities, so that the effect of the feed additive on resisting pathogenic vibrio of penaeus vannamei is improved to a certain extent.
Further, the preparation method of the clostridium butyricum fermentation preparation comprises the following steps:
A1. preparing clostridium butyricum fermentation broth: inoculating clostridium butyricum into an RCM liquid culture medium, controlling the mass ratio of clostridium butyricum to the RCM liquid culture medium to be (4-5): 100, and carrying out anaerobic fermentation at 32-37 ℃ for 1-2d to obtain clostridium butyricum fermentation broth; the clostridium butyricum fermentation liquid contains metabolites including butyric acid, pyruvic acid, lactic acid, acetic acid, a small amount of organic acids such as propionic acid and formic acid, can penetrate the cell wall of the gram-negative vibrio, dissociate in cytoplasm and interfere the function of vibrio cells, and further has a certain broad-spectrum vibrio resistance; meanwhile, the metabolite also has certain volatile aroma, so that the palatability and the food calling effect of the feed are improved; in addition, clostridium butyricum has a competitive relationship with pathogenic vibrio parasitized in the intestinal tracts of the penaeus vannamei, so that the growth and propagation of vibrio are limited, the proliferation of beneficial bacterial flora in the intestinal tracts of the penaeus vannamei is promoted, the immune function is enhanced, and the incidence probability of enteritis of the penaeus vannamei is reduced;
A2. preparing clostridium butyricum fermentation preparation: and freeze-drying the clostridium butyricum fermentation broth to obtain a clostridium butyricum fermentation preparation.
Further, the preparation method of the Phellinus linteus fermentation preparation comprises the following steps:
B1. inoculating Phellinus linteus mycelium into Phellinus linteus liquid culture medium, controlling the mass ratio of Phellinus linteus mycelium to Phellinus linteus liquid culture medium to 100 (0.2-0.4), aerobically fermenting at 28-32deg.C for 7-10d, and separating Phellinus linteus thallus and fermented liquid after filter pressing;
B2. drying Phellinus linteus thallus, and superfine pulverizing to particle size of 5-10 μm to obtain Phellinus linteus thallus superfine powder;
B3. adding the obtained superfine powder of mulberry Huang Junti into the separated fermentation liquid, and performing ultrasonic treatment at 60-70 ℃ for 1-2 hours to obtain a Phellinus linteus fermentation liquid; superfine pulverizing Phellinus Linteus, and ultrasonic treating to improve the dissolution rate and extraction rate of active ingredients such as mycelium intracellular polysaccharide, fruiting body polysaccharide and flavone;
B4. concentrating the Phellinus linteus fermentation liquid under reduced pressure, and spray drying to obtain Phellinus linteus fermentation preparation; the Phellinus linteus fermentation preparation is rich in Phellinus linteus polysaccharide, and active ingredients such as flavonoid compounds (mainly including flavan and flavan-3-ol flavone), terpenoid compounds (mainly including sesquiterpene, diterpene and triterpene), pyrone compounds (having styryl pyrone skeleton and benzopyrone skeleton structure), steroid compounds (mainly including hordeolum stanol steroid), etc., and has antibacterial, immunoregulatory, antioxidant, free radical scavenging, and Penaeus vannamei Boone damaged cell and tissue repairing effects.
Further, the liquid culture medium for Phellinus linteus comprises the following formula: glucose 20-25g/L, lignocellulose 25-30g/L, potato powder 2-3g/L, peptone 3-5g/L, KH 2 PO 4 0.5-1g/L、K 2 HPO 4 0.1-0.5g/L，pH＝6.7-6.9。
The invention further provides a preparation method of the feed additive for penaeus vannamei boone cultivation, which comprises the following steps:
s1: adding the total amino beta-cyclodextrin and the microcapsule auxiliary agent into deionized water, carrying out ultrasonic treatment for 0.5-1h, then adding polyoxyethylene 40 hydrogenated castor oil, and uniformly stirring to obtain a wall material solution;
s2: uniformly mixing the first vibrio inhibitor, the second vibrio inhibitor and the mixed fermentation preparation to obtain a core material mixture;
s3: adding the core material mixture into the wall material solution, stirring at 32-37 ℃ for 0.5-1h, and freeze-drying to obtain the feed additive for penaeus vannamei boone cultivation; in the process, the total amino beta-cyclodextrin can also have a crosslinking effect with moringa seed element and vanillin in the first vibrio inhibitor, so that the embedding stability and the structural stability of the prepared microcapsule feed additive are further enhanced; at the same time, amino in the total amino beta-cyclodextrin reacts with aldehyde carbonyl in vanillin to form a derivative with a Schiff base structure, so that the effect of resisting pathogenic vibrio of penaeus vannamei is further enhanced.
The invention also provides an application of the feed additive for penaeus vannamei boone cultivation prepared by the preparation method in penaeus vannamei boone feed, and the dosage of the feed additive is 0.1-0.15% of the penaeus vannamei boone feed.
The invention has the beneficial effects that:
the feed additive prepared by the invention can effectively inhibit pathogenic vibrio in water, improve the survival rate of the penaeus vannamei boone, avoid drug resistance, avoid pollution of antibiotics to the culture water, and is beneficial to improving the feeding rate of the penaeus vannamei boone to the feed so as to improve the effective utilization rate of the feed. Specifically:
according to the invention, the moringa seed extract and the vanillin are combined with the first vibrio inhibitor for the first time, the dill essential oil is used as the second vibrio inhibitor, and the three components are synergistic, so that the performance of resisting pathogenic vibrio and the survival rate of the penaeus vannamei boone are obviously improved, the fragrance is improved, and the food calling effect is achieved.
The invention improves the intestinal environment of the penaeus vannamei boone by scientifically and reasonably combining the Phellinus linteus fermenting preparation and the Clostridium butyricum fermenting preparation, further improves the inhibiting effect on pathogenic vibrio and the immunity function, and further improves the survival rate of the penaeus vannamei boone.
Finally, embedding treatment is carried out by utilizing the full amino beta-cyclodextrin and the dialdehyde starch to form a compact and stable microcapsule structure, so that invasion of external miscellaneous bacteria is prevented, loss of volatile aroma components in the feed additive is avoided, meanwhile, after entering a culture water body, active ingredients are slowly released, and the inhibition time and the food calling time of the feed additive on vibrio are prolonged.
Drawings
In order to more clearly illustrate the invention or the technical solutions of the prior art, the drawings which are used in the description of the embodiments or the prior art will be briefly described, it being obvious that the drawings in the description below are only of the invention and that other drawings can be obtained from them without inventive effort for a person skilled in the art.
FIG. 1 is a flow chart of a preparation method of the feed additive for penaeus vannamei boone cultivation.
Detailed Description
The present invention will be further described in detail with reference to specific embodiments in order to make the objects, technical solutions and advantages of the present invention more apparent.
Example 1
A preparation method of a feed additive for penaeus vannamei boone cultivation comprises the following steps:
s1: preparing a first vibrio inhibitor: uniformly mixing moringa seed extract and vanillin according to a mass ratio of 1:0.2 to obtain a first vibrio inhibitor;
s2: preparing a microcapsule auxiliary agent: uniformly mixing dialdehyde starch and triethylamine according to the mass ratio of 1:0.1 to obtain a microcapsule auxiliary agent;
s3: preparing a mixed fermentation preparation:
s31: preparing clostridium butyricum fermentation preparation:
s311: preparing clostridium butyricum fermentation broth: inoculating clostridium butyricum into an RCM liquid culture medium, controlling the mass ratio of clostridium butyricum to the RCM liquid culture medium to be 4:100, and carrying out anaerobic fermentation at 32 ℃ for 1d to obtain clostridium butyricum fermentation liquor;
s312: preparing clostridium butyricum fermentation preparation: freeze-drying the clostridium butyricum fermentation broth to obtain clostridium butyricum fermentation preparation;
s32: preparing a Phellinus linteus fermentation preparation:
s321: the Phellinus linteus liquid culture medium is prepared according to the following formula: glucose 20g/L, lignocellulose 25g/L, potato powder 2g/L, peptone 3g/L, KH 2 PO 4 0.5g/L、K 2 HPO 4 0.1g/L，pH＝6.7；
S322: inoculating Phellinus linteus mycelium into Phellinus linteus liquid culture medium, controlling the mass ratio of Phellinus linteus mycelium to Phellinus linteus liquid culture medium to 0.2:100, aerobically fermenting at 28deg.C for 7d under ventilation condition, and separating Phellinus linteus thallus and fermentation liquid after filter pressing;
s323: drying Phellinus linteus thallus, and superfine pulverizing to particle size of 5 μm to obtain Phellinus linteus thallus superfine powder;
s324: adding the obtained mulberry Huang Junti superfine powder into the separated fermentation liquid, and carrying out ultrasonic treatment at 60 ℃ for 1h to obtain a Phellinus linteus fermentation liquid;
s325: concentrating the Phellinus linteus fermentation liquid under reduced pressure, and spray drying to obtain Phellinus linteus fermentation preparation;
s33: uniformly mixing clostridium butyricum fermentation preparation and Phellinus linteus fermentation preparation according to the mass ratio of 1:0.15 to obtain a mixed fermentation preparation;
s4: adding 5g of total amino beta-cyclodextrin and 1g of microcapsule auxiliary agent into 50g of deionized water, carrying out ultrasonic treatment for 0.5h, adding 0.3g of polyoxyethylene 40 hydrogenated castor oil, and uniformly stirring to obtain a wall material solution;
s5: uniformly mixing 3g of a first vibrio inhibitor, 1g of a second vibrio inhibitor dill essential oil and 10g of a mixed fermentation preparation to obtain a core material mixture;
s6: and (3) adding the core material mixture obtained in the step (S5) into the wall material solution obtained in the step (S4), stirring at 32 ℃ for 0.5h, and then, freeze-drying to obtain the feed additive for penaeus vannamei boone cultivation.
Example 2
A preparation method of a feed additive for penaeus vannamei boone cultivation comprises the following steps:
s1: preparing a first vibrio inhibitor: uniformly mixing moringa seed extract and vanillin according to a mass ratio of 1:0.35 to obtain a first vibrio inhibitor;
s2: preparing a microcapsule auxiliary agent: uniformly mixing dialdehyde starch and triethylamine according to the mass ratio of 1:0.13 to obtain a microcapsule auxiliary agent;
s3: preparing a mixed fermentation preparation:
s31: preparing clostridium butyricum fermentation preparation:
s311: preparing clostridium butyricum fermentation broth: inoculating clostridium butyricum into an RCM liquid culture medium, controlling the mass ratio of clostridium butyricum to the RCM liquid culture medium to be 4.5:100, and carrying out anaerobic fermentation at 35 ℃ for 1.5d to obtain clostridium butyricum fermentation broth;
s312: preparing clostridium butyricum fermentation preparation: freeze-drying the clostridium butyricum fermentation broth to obtain clostridium butyricum fermentation preparation;
s32: preparing a Phellinus linteus fermentation preparation:
s321: the Phellinus linteus liquid culture medium is prepared according to the following formula: glucose 22.5g/L, lignocellulose 27.5g/L, potato powder 2.5g/L, peptone 4g/L, KH 2 PO 4 0.75g/L、K 2 HPO 4 0.3g/L，pH＝6.8；
S322: inoculating Phellinus linteus mycelium into Phellinus linteus liquid culture medium, controlling the mass ratio of Phellinus linteus mycelium to Phellinus linteus liquid culture medium to 0.3:100, aerobically fermenting at 30deg.C under ventilation condition for 8d, and separating Phellinus linteus thallus and fermentation liquid after filter pressing;
s323: drying Phellinus linteus thallus, and superfine pulverizing to particle size of 7.5 μm to obtain Phellinus linteus thallus superfine powder;
s324: adding the obtained superfine powder of the mulberry Huang Junti into the separated fermentation liquid, and carrying out ultrasonic treatment at 65 ℃ for 1-2 hours to obtain a Phellinus linteus fermentation liquid;
s325: concentrating the Phellinus linteus fermentation liquid under reduced pressure, and spray drying to obtain Phellinus linteus fermentation preparation;
s33: uniformly mixing clostridium butyricum fermentation preparation and Phellinus linteus fermentation preparation according to the mass ratio of 1:0.25 to obtain a mixed fermentation preparation;
s4: adding 6g of total amino beta-cyclodextrin and 1.5g of microcapsule auxiliary agent into 60g of deionized water, carrying out ultrasonic treatment for 1h, adding 0.4g of polyoxyethylene 40 hydrogenated castor oil, and uniformly stirring to obtain a wall material solution;
s5: uniformly mixing 5g of a first vibrio inhibitor, 2g of a second vibrio inhibitor dill essential oil and 12.5g of a mixed fermentation preparation to obtain a core material mixture;
s6: and (3) adding the core material mixture obtained in the step (S5) into the wall material solution obtained in the step (S4), stirring at 35 ℃ for 1h, and then performing freeze drying to obtain the feed additive for penaeus vannamei boone cultivation.
Example 3
A preparation method of a feed additive for penaeus vannamei boone cultivation comprises the following steps:
s1: preparing a first vibrio inhibitor: uniformly mixing moringa seed extract and vanillin according to a mass ratio of 1:0.5 to obtain a first vibrio inhibitor;
s2: preparing a microcapsule auxiliary agent: uniformly mixing dialdehyde starch and triethylamine according to the mass ratio of 1:0.15 to obtain a microcapsule auxiliary agent;
s3: preparing a mixed fermentation preparation:
s31: preparing clostridium butyricum fermentation preparation:
s311: preparing clostridium butyricum fermentation broth: inoculating clostridium butyricum into an RCM liquid culture medium, controlling the mass ratio of clostridium butyricum to the RCM liquid culture medium to be 5:100, and carrying out anaerobic fermentation at 37 ℃ for 2d to obtain clostridium butyricum fermentation liquor;
s312: preparing clostridium butyricum fermentation preparation: freeze-drying the clostridium butyricum fermentation broth to obtain clostridium butyricum fermentation preparation;
s32: preparing a Phellinus linteus fermentation preparation:
s321: the Phellinus linteus liquid culture medium is prepared according to the following formula: glucose 25g/L, lignocellulose 30g/L, potato powder 3g/L, peptone 5g/L, KH 2 PO 4 1g/L、K 2 HPO 4 0.5g/L，pH＝6.9；
S322: inoculating Phellinus linteus mycelium into Phellinus linteus liquid culture medium, controlling the mass ratio of Phellinus linteus mycelium to Phellinus linteus liquid culture medium to 0.4:100, aerobically fermenting at 32deg.C for 10d under ventilation condition, and separating Phellinus linteus thallus and fermentation liquid after filter pressing;
s323: drying Phellinus linteus thallus, and micronizing to particle size of 10 μm to obtain Phellinus linteus thallus micropowder;
s324: adding the obtained superfine powder of the mulberry Huang Junti into the separated fermentation liquid, and carrying out ultrasonic treatment at 70 ℃ for 2 hours to obtain a Phellinus linteus fermentation liquid;
s325: concentrating the Phellinus linteus fermentation liquid under reduced pressure, and spray drying to obtain Phellinus linteus fermentation preparation;
s33: uniformly mixing clostridium butyricum fermentation preparation and Phellinus linteus fermentation preparation according to the mass ratio of 1:0.3 to obtain a mixed fermentation preparation;
s4: adding 7g of total amino beta-cyclodextrin and 2g of microcapsule auxiliary agent into 70g of deionized water, carrying out ultrasonic treatment for 1h, adding 0.5g of polyoxyethylene 40 hydrogenated castor oil, and uniformly stirring to obtain a wall material solution;
s5: uniformly mixing 7g of a first vibrio inhibitor, 3g of a second vibrio inhibitor dill essential oil and 15g of a mixed fermentation preparation to obtain a core material mixture;
s6: and (3) adding the core material mixture obtained in the step (S5) into the wall material solution obtained in the step (S4), stirring at 37 ℃ for 1h, and then performing freeze drying to obtain the feed additive for penaeus vannamei boone cultivation.
Comparative example 1 is the same as example 1 except that the first vibrio inhibitor is not added during the preparation of S5.
Comparative example 2 is the same as example 1 except that dill essential oil, a second vibrio inhibitor, is not added during the preparation of S5.
Comparative example 3 is the same as example 1 except that moringa seed is used in place of the first vibrio inhibitor in example 1.
Comparative example 4 is the same as example 1 except that vanillin is substituted for the first vibrio inhibitor in example 1.
Comparative example 5 is the same as example 1 except that no microcapsule auxiliary is added during the preparation of S4.
Comparative example 6 is the same as example 1 except that beta-cyclodextrin is used instead of the full amino beta-cyclodextrin and no microcapsule auxiliary is added during the preparation of S4.
Comparative example 7 is the same as example 1 except that the mixed fermentation preparation in example 1 is replaced with a clostridium butyricum fermentation preparation.
Test one: and (3) testing the culture effect of the feed applied to the penaeus vannamei boone feed:
the feed additives prepared in examples 1-3 and comparative examples 1-7 were added to the same feed for penaeus vannamei, the amount of the feed additive was 0.1% of the mass of the feed for penaeus vannamei, the feed for penaeus vannamei was purchased from the company of the biological technology of juner-ruixing, the penaeus vannamei in the growth period of PL10 was selected, the number of the penaeus vannamei breeds in each group was 200, 3 parallel experiments were set up for each group, the feeding was carried out 3 times per day, the feeding time was 6:00, 13:00, 21:00, and the continuous breeding was 28d, and the survival rate and feed coefficient were calculated, the calculation formula was as follows:
survival = 100% × (live shrimp mantissa after 28 d-initial shrimp mantissa)/initial shrimp mantissa, initial shrimp mantissa = 200;
feed factor = total feed amount/(final body weight after 28 d-initial body weight);
the cultivation test results are shown in table 1:
TABLE 1
As can be seen from Table 1, the feed additives prepared in examples 1-3 significantly improve the survival rate of Penaeus vannamei Boone and the effective utilization rate of the compound feed, and demonstrate that the feed additives prepared in the invention have excellent effects of resisting pathogenic bacteria and inducing food.
And (2) testing II: determination of Vibrio resistance:
vibrio cholerae and Vibrio parahaemolyticus are used as test vibrio strains, and the vibrio cholerae and Vibrio parahaemolyticus are diluted to 10 by using sterile water respectively 6 CFU/mL, 100mL of LB liquid medium is taken as a blank group, 100mL of LB liquid medium added with the feed additives prepared in examples 1-3 and comparative examples 1-7 is taken as 10 test groups, the feed additive is 0.1% of the mass of the LB liquid medium, 200 mu L of vibrio test diluent is inoculated into the blank group and the test group, shake cultivation is carried out for 72 hours at 37 ℃ and 200r/min, and calculation is carried out according to the following formula: vibrio inhibition ratio = 100% × (amount of vibrio bacteria after the culture of the blank control group-amount of vibrio bacteria after the culture of the test group)/amount of vibrio bacteria after the culture of the blank control group. The test results of the anti-vibrio performance test are shown in table 2:
TABLE 2
As is clear from Table 2, the feed additives prepared in examples 1 to 3 have excellent anti-pathogenic vibrio properties, and the inhibitory properties against Vibrio cholerae and Vibrio parahaemolyticus are more than 97%.
And (3) test III: stability test:
after the feed additives prepared in examples 1 to 3 and comparative examples 5 to 6 were exposed to 90RH and 25℃for 7 days, the feed additives prepared in comparative examples 1 to 3 and comparative examples 5 to 6 were subjected to the conditions of difference in fragrance and deliquescence, and the test results are shown in Table 3:
TABLE 3 Table 3
As can be seen from Table 3, the feed additives prepared in examples 1-3 can retain aroma components of the feed additives in the environment of 90RH and 25 ℃, while the feed additives prepared in comparative examples 5-6 are more deliquescent and the aroma components are also relatively easier to lose, which demonstrates that examples 1-3 improve compactness and stability of the outer wall material through the crosslinking action of the total amino beta-cyclodextrin and the microcapsule auxiliary agent.
Those of ordinary skill in the art will appreciate that: the discussion of any of the embodiments above is merely exemplary and is not intended to suggest that the scope of the invention (including the claims) is limited to these examples; the technical features of the above embodiments or in the different embodiments may also be combined within the idea of the invention, the steps may be implemented in any order and there are many other variations of the different aspects of the invention as described above, which are not provided in detail for the sake of brevity.
The present invention is intended to embrace all such alternatives, modifications and variances which fall within the broad scope of the appended claims. Therefore, any omission, modification, equivalent replacement, improvement, etc. of the present invention should be included in the scope of the present invention.
Claims (10)
1. The feed additive for penaeus vannamei boone cultivation is characterized by comprising the following raw materials in parts by mass: 3-7 parts of a first vibrio inhibitor, 1-3 parts of a second vibrio inhibitor, 5-7 parts of total amino beta-cyclodextrin, 1-2 parts of a microcapsule auxiliary agent, 0.3-0.5 part of polyoxyethylene 40 hydrogenated castor oil, 10-15 parts of a mixed fermentation preparation and 50-70 parts of deionized water;
the first vibrio inhibitor is prepared by mixing moringa seed extract and vanillin according to a mass ratio of 1 (0.2-0.5);
the second vibrio inhibitor is dill essential oil;
the mixed fermentation preparation is prepared by mixing clostridium butyricum fermentation preparation and Phellinus linteus fermentation preparation according to the mass ratio of 1 (0.15-0.3).
2. The feed additive for penaeus vannamei boone cultivation as claimed in claim 1, which is characterized by comprising the following raw materials in parts by mass: 3 parts of a first vibrio inhibitor, 1 part of a second vibrio inhibitor, 5 parts of total amino beta-cyclodextrin, 1 part of a microcapsule auxiliary agent, 0.3 part of polyoxyethylene 40 hydrogenated castor oil, 10 parts of a mixed fermentation preparation and 50 parts of deionized water;
the first vibrio inhibitor is formed by mixing moringa seed extract and vanillin according to the mass ratio of 1:0.2;
the second vibrio inhibitor is dill essential oil;
the mixed fermentation preparation is prepared by mixing clostridium butyricum fermentation preparation and Phellinus linteus fermentation preparation according to the mass ratio of 1:0.15.
3. The feed additive for penaeus vannamei boone cultivation as claimed in claim 1, which is characterized by comprising the following raw materials in parts by mass: comprises the following raw materials in parts by mass: 5 parts of a first vibrio inhibitor, 2 parts of a second vibrio inhibitor, 6 parts of total amino beta-cyclodextrin, 1.5 parts of a microcapsule auxiliary agent, 0.4 part of polyoxyethylene 40 hydrogenated castor oil, 12.5 parts of a mixed fermentation preparation and 60 parts of deionized water;
the first vibrio inhibitor is prepared by mixing moringa seed extract and vanillin according to a mass ratio of 1:0.35;
the second vibrio inhibitor is dill essential oil;
the mixed fermentation preparation is prepared by mixing clostridium butyricum starter and Phellinus linteus starter according to the mass ratio of 1:0.25.
4. The feed additive for penaeus vannamei boone cultivation as claimed in claim 1, which is characterized by comprising the following raw materials in parts by mass: comprises the following raw materials in parts by mass: 7 parts of a first vibrio inhibitor, 3 parts of a second vibrio inhibitor, 7 parts of total amino beta-cyclodextrin, 2 parts of a microcapsule auxiliary agent, 0.5 part of polyoxyethylene 40 hydrogenated castor oil, 15 parts of a mixed fermentation preparation and 70 parts of deionized water;
the first vibrio inhibitor is prepared by mixing moringa seed extract and vanillin according to a mass ratio of 1:0.5;
the second vibrio inhibitor is dill essential oil;
the mixed fermentation preparation is prepared by mixing clostridium butyricum fermentation preparation and Phellinus linteus fermentation preparation according to the mass ratio of 1:0.3.
5. The feed additive for penaeus vannamei boone cultivation as claimed in claim 1, wherein the microcapsule auxiliary agent is formed by mixing dialdehyde starch and triethylamine according to a mass ratio of 1 (0.1-0.15).
6. The feed additive for penaeus vannamei boone culture of claim 1, wherein the preparation method of the clostridium butyricum fermentation preparation comprises the following steps:
A1. preparing clostridium butyricum fermentation broth: inoculating clostridium butyricum into an RCM liquid culture medium, controlling the mass ratio of clostridium butyricum to the RCM liquid culture medium to be (4-5): 100, and carrying out anaerobic fermentation at 32-37 ℃ for 1-2d to obtain clostridium butyricum fermentation broth;
A2. preparing clostridium butyricum fermentation preparation: and freeze-drying the clostridium butyricum fermentation broth to obtain a clostridium butyricum fermentation preparation.
7. The feed additive for penaeus vannamei boone cultivation as claimed in claim 1, wherein the preparation method of the Phellinus linteus fermentation preparation comprises the following steps:
B1. inoculating Phellinus linteus mycelium into Phellinus linteus liquid culture medium, controlling the mass ratio of Phellinus linteus mycelium to Phellinus linteus liquid culture medium to 100 (0.2-0.4), aerobically fermenting at 28-32deg.C for 7-10d, and separating Phellinus linteus thallus and fermented liquid after filter pressing;
B2. drying Phellinus linteus thallus, and superfine pulverizing to particle size of 5-10 μm to obtain Phellinus linteus thallus superfine powder;
B3. adding the obtained superfine powder of mulberry Huang Junti into the separated fermentation liquid, and performing ultrasonic treatment at 60-70 ℃ for 1-2 hours to obtain a Phellinus linteus fermentation liquid;
B4. concentrating the Phellinus linteus fermentation liquid under reduced pressure, and spray drying to obtain Phellinus linteus fermentation preparation.
8. The feed additive for penaeus vannamei boone cultivation as claimed in claim 7, wherein the liquid culture medium for Phellinus linteus comprises the following formula: glucose 20-25g/L, lignocellulose 25-30g/L, potato powder 2-3g/L, peptone 3-5g/L, KH 2 PO 4 0.5-1g/L、K 2 HPO 4 0.1-0.5g/L，pH＝6.7-6.9。
9. A method for preparing the feed additive for penaeus vannamei boone culture according to any one of claims 1-8, comprising the following steps:
s1: adding the total amino beta-cyclodextrin and the microcapsule auxiliary agent into deionized water, carrying out ultrasonic treatment for 0.5-1h, then adding polyoxyethylene 40 hydrogenated castor oil, and uniformly stirring to obtain a wall material solution;
s2: uniformly mixing the first vibrio inhibitor, the second vibrio inhibitor and the mixed fermentation preparation to obtain a core material mixture;
s3: adding the core material mixture into the wall material solution, stirring at 32-37 ℃ for 0.5-1h, and freeze-drying to obtain the feed additive for penaeus vannamei boone cultivation.
10. The application of the feed additive for penaeus vannamei boone cultivation prepared by the preparation method of claim 9 in penaeus vannamei boone feed, which is characterized in that: the feed additive is 0.1-0.15% of the feed mass of the penaeus vannamei boone.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311088895.4A CN116998635A (en) | 2023-08-28 | 2023-08-28 | Feed additive for penaeus vannamei boone cultivation and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311088895.4A CN116998635A (en) | 2023-08-28 | 2023-08-28 | Feed additive for penaeus vannamei boone cultivation and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116998635A true CN116998635A (en) | 2023-11-07 |
Family
ID=88569063
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311088895.4A Pending CN116998635A (en) | 2023-08-28 | 2023-08-28 | Feed additive for penaeus vannamei boone cultivation and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116998635A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003045161A1 (en) * | 2001-11-29 | 2003-06-05 | Nc Biotec Co., Ltd. | A preparation method of feed and feed additive using mushroom mycelium |
| WO2016153247A1 (en) * | 2015-03-26 | 2016-09-29 | 한국생명공학연구원 | Clostridium butyricum strain having immune enhancement and antiviral activities, and use thereof |
| CN107494998A (en) * | 2017-09-19 | 2017-12-22 | 清远海贝生物技术有限公司 | A kind of composite feed additive for suppressing Penaeus Vannmei enteron aisle vibrios |
| CN109463530A (en) * | 2018-11-08 | 2019-03-15 | 浙江省农业科学院 | A kind of feed additive for promoting growth and preparation method thereof of regulation enteric microorganism |
| CN112690373A (en) * | 2020-12-25 | 2021-04-23 | 福建傲农生物科技集团股份有限公司 | Composition for preventing white spot syndrome of prawns as well as preparation method and application thereof |
| CN114231434A (en) * | 2021-10-13 | 2022-03-25 | 南宁市拜欧生物工程有限责任公司 | Clostridium butyricum for feed additive and application thereof |
| CN114672446A (en) * | 2022-05-31 | 2022-06-28 | 广东海洋大学 | Preparation method and application of clostridium butyricum preparation |
-
2023
- 2023-08-28 CN CN202311088895.4A patent/CN116998635A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003045161A1 (en) * | 2001-11-29 | 2003-06-05 | Nc Biotec Co., Ltd. | A preparation method of feed and feed additive using mushroom mycelium |
| WO2016153247A1 (en) * | 2015-03-26 | 2016-09-29 | 한국생명공학연구원 | Clostridium butyricum strain having immune enhancement and antiviral activities, and use thereof |
| CN107494998A (en) * | 2017-09-19 | 2017-12-22 | 清远海贝生物技术有限公司 | A kind of composite feed additive for suppressing Penaeus Vannmei enteron aisle vibrios |
| CN109463530A (en) * | 2018-11-08 | 2019-03-15 | 浙江省农业科学院 | A kind of feed additive for promoting growth and preparation method thereof of regulation enteric microorganism |
| CN112690373A (en) * | 2020-12-25 | 2021-04-23 | 福建傲农生物科技集团股份有限公司 | Composition for preventing white spot syndrome of prawns as well as preparation method and application thereof |
| CN114231434A (en) * | 2021-10-13 | 2022-03-25 | 南宁市拜欧生物工程有限责任公司 | Clostridium butyricum for feed additive and application thereof |
| CN114672446A (en) * | 2022-05-31 | 2022-06-28 | 广东海洋大学 | Preparation method and application of clostridium butyricum preparation |
Non-Patent Citations (1)
| Title |
|---|
| 杨平荣 等: "《中草药植物提取与深加工新技术实用手册》", vol. 1, 上海科学普及出版社, pages: 103 - 104 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Elbanna et al. | Microbiological, histological, and biochemical evidence for the adverse effects of food azo dyes on rats | |
| AU2002244694B2 (en) | Symbiotic regenerative agent | |
| JP6812545B2 (en) | A feed composition for the prevention or treatment of acute hepatopancreatic necrosis (AHPND) or white spot disease syndrome (WSS) containing Bacillus subtilis strain, Bacillus pumilus strain and Bacillus licheniformis strain as active ingredients. | |
| CN101485789A (en) | Camellia chrysantha polysaccharide lozenge and method for producing the same | |
| US20220160796A1 (en) | Composition for preventing, alleviating, or treating obesity or fatty liver disease, comprising weissella hellenica wikim0103 | |
| CN107789370B (en) | Agent for preventing and treating alcoholic liver disease | |
| CN115944665A (en) | Probiotic agent for improving intestinal flora balance and preparation method and application thereof | |
| KR102567035B1 (en) | Lactobacillus fermentum LF-SCHY34 and its application | |
| CN112970975A (en) | Mixed eel feed additive for increasing proportion of red fish meal in feed, preparation method and application thereof | |
| CN116998635A (en) | Feed additive for penaeus vannamei boone cultivation and preparation method and application thereof | |
| CN115463159A (en) | Application of Acermanium and Acermanium postbiotic in preparation of medicine or health-care product for preventing or treating hepatic steatosis | |
| KR102589163B1 (en) | Manufacturing method of sandwich-coated lactic acid bacteria containing aloe polysaccharide and sandwich-coated lactic acid bacteria containing aloe polysaccharide prepared thereby | |
| KR102139443B1 (en) | Composition for inhibiting adipogenesis comprising Chrysanthemum indicum fermented by lactic acid bacteria | |
| Miltko et al. | Can fungal zoospores be the source of energy for the rumen protozoa Eudiplodinium maggii? | |
| JP3649787B2 (en) | Prophylactic agent for enterococcal infection in fish and use thereof | |
| KR101228554B1 (en) | The Fermentation Forming Method of Citrus Sunki And The Product thereof | |
| CN115226811B (en) | Feed preservative and application thereof in preparation of cordyceps sinensis larva feed | |
| KR20160007744A (en) | Composition for anti-hangover and anti-obesity containing extract of Mytilus edulis | |
| dos S Filho et al. | Immunostimulants derived from plants and algae to increase resistance of pacific white shrimp (Litopenaeus vannamei) against vibriosis | |
| CN112273552B (en) | Feed additive and preparation method and application thereof | |
| CN114304688B (en) | Zein coating agent, probiotics preparation coated by coating agent and preparation method thereof | |
| CN115316539B (en) | Nutritional feed for suckling pigs in transitional period and preparation method thereof | |
| JP6381284B2 (en) | Composition for prevention and / or treatment of memory impairment | |
| KR102169878B1 (en) | Fermented glutinous rice product and food or pharmaceutical composition comprising the same | |
| WO2022080516A1 (en) | Composition for hangover relief and liver function improvement |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |