CN116965325B - Method for obtaining hybrid seeds and seed germination of iris Netherlands - Google Patents
Method for obtaining hybrid seeds and seed germination of iris Netherlands Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 24
- 230000007226 seed germination Effects 0.000 title claims abstract description 9
- 238000009396 hybridization Methods 0.000 claims abstract description 32
- 238000005286 illumination Methods 0.000 claims abstract description 15
- 239000004576 sand Substances 0.000 claims abstract description 15
- 239000002775 capsule Substances 0.000 claims abstract description 13
- 238000009331 sowing Methods 0.000 claims abstract description 12
- 238000001035 drying Methods 0.000 claims abstract description 11
- 238000012258 culturing Methods 0.000 claims abstract description 8
- 238000009423 ventilation Methods 0.000 claims abstract description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 19
- 239000004677 Nylon Substances 0.000 claims description 12
- 229920001778 nylon Polymers 0.000 claims description 12
- 239000002689 soil Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 9
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 9
- 239000006013 carbendazim Substances 0.000 claims description 9
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims description 9
- 239000004033 plastic Substances 0.000 claims description 7
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 6
- 238000002791 soaking Methods 0.000 claims description 6
- 238000005406 washing Methods 0.000 claims description 6
- 239000007864 aqueous solution Substances 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 229920006395 saturated elastomer Polymers 0.000 claims description 5
- 241001494479 Pecora Species 0.000 claims description 3
- 229920001131 Pulp (paper) Polymers 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 3
- 230000032669 eclosion Effects 0.000 claims description 3
- 210000003608 fece Anatomy 0.000 claims description 3
- 239000003337 fertilizer Substances 0.000 claims description 3
- 239000010871 livestock manure Substances 0.000 claims description 3
- 239000002985 plastic film Substances 0.000 claims description 3
- 229920006255 plastic film Polymers 0.000 claims description 3
- 238000003971 tillage Methods 0.000 claims description 3
- 229920000742 Cotton Polymers 0.000 claims description 2
- 210000000349 chromosome Anatomy 0.000 claims description 2
- 210000001082 somatic cell Anatomy 0.000 claims description 2
- 241000894007 species Species 0.000 claims 1
- 230000010152 pollination Effects 0.000 abstract description 5
- 238000003306 harvesting Methods 0.000 abstract description 4
- 230000001737 promoting effect Effects 0.000 abstract description 4
- 238000009395 breeding Methods 0.000 abstract description 3
- 230000001488 breeding effect Effects 0.000 abstract description 3
- 230000001149 cognitive effect Effects 0.000 abstract description 3
- 230000007547 defect Effects 0.000 abstract description 3
- 238000011161 development Methods 0.000 abstract description 3
- 230000018109 developmental process Effects 0.000 abstract description 3
- 241000196324 Embryophyta Species 0.000 description 8
- 240000002853 Nelumbo nucifera Species 0.000 description 7
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 7
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 206010021928 Infertility female Diseases 0.000 description 2
- 206010021929 Infertility male Diseases 0.000 description 2
- 208000007466 Male Infertility Diseases 0.000 description 2
- 230000003044 adaptive effect Effects 0.000 description 2
- 230000008676 import Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000003892 spreading Methods 0.000 description 2
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 239000005644 Dazomet Substances 0.000 description 1
- 241001113425 Iridaceae Species 0.000 description 1
- 244000071493 Iris tectorum Species 0.000 description 1
- 241000822723 Iris tuberosa Species 0.000 description 1
- 241001495668 Iris x hollandica Species 0.000 description 1
- 230000001174 ascending effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009402 cross-breeding Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
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- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Health & Medical Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for obtaining hybrid seeds and seed germination of iris lilacina, which comprises the steps of selecting a specific variety as a hybrid parent and planting; when the flowers are just opened and are not scattered, performing matched hybridization pollination, and repeating hybridization pollination after 2-3 days; harvesting and drying in a ventilation place after capsules produced by hybridization are changed into yellow and mature; selecting full seeds, sowing the seeds in a basin filled with yellow sand, and culturing the seeds in a climatic chamber until the seeds germinate, wherein the culturing conditions are as follows: the illumination time is 16h/d, the temperature is 23+/-1 ℃ during illumination, the temperature is 18+/-1 ℃ during darkness, and the illumination intensity is 20000-25000Lux. The method can lead the hybrid setting rate of the iris lilacina to be more than 95 percent and the seed germination rate to be more than 10 percent, overcomes the traditional cognitive defect that the iris lilacina planted in China is not set, and has important milestone significance for promoting the breeding process of the iris lilacina in China and promoting the development of the industry.
Description
Technical Field
The invention belongs to the field of plant genetic breeding, and particularly relates to a method for obtaining hybrid seeds and seed germination of iris Netherlands.
Background
The Netherlands Iris (Iris x hollandica) is a bulb flower of Iris genus of Iridaceae family, is the most widely cultivated and applied type of bulb Iris, has a natural flowering period of 4 months and a group flowering period of about 30 days, is mainly used for cutting peanut production at present, but can also be used in various forms such as potted flowers, flower fields, flowers, forests and the like. The first introduction of the cultivated iris Netherlands in the last 90 th century in China, but the application and breeding research of the iris Netherlands are less, and the fresh cut flowers sold on the market almost depend on import or seed ball import planting production. With the continuous development of social economy, the requirements of the lotus iris as a high-grade cut flower in China are increased year by year in recent years, and compared with other imported bulb flowers, the lotus iris seed balls are less degraded, can be continuously planted for a plurality of years after being introduced once, and do not need to purchase the seed balls year by year, so that the lotus iris seed ball has wide market prospect.
However, only a few new varieties using physical mutagenesis or natural mutation are reported in China at present, and the reason is mainly that most of the iris netherlands varieties have female or male sterility characteristics, so that cross breeding is difficult. Therefore, how to obtain hybrid seeds and seedlings of iris Netherlands is a technical problem to be solved by the technicians in the field.
Disclosure of Invention
The invention aims to provide a method for obtaining iris Netherlands hybrid seeds and germinating seeds.
It is to be noted that the invention discovers that the hybrid offspring can be produced by selecting specific parents for matching on the basis of carrying out adaptive planting and artificial matching in a large number of foreign fine varieties in Jiangsu regions, and the hybrid seeds can germinate to obtain offspring seedlings. The research result of the invention makes up the traditional cognitive defect of the infirm of the domestic planted iris.
Based on the above purpose, the invention adopts the following technical scheme:
a method for obtaining hybrid seeds and seed germination of iris Netherlands comprises the following steps:
(1) Selection of hybrid parents: most varieties of iris Netherlands have female or male sterility, so that reports of obtaining iris Netherlands seeds do not appear so far in China, but on the basis of massive introduction, grouping and adaptive cultivation, the hybrid seeds can be obtained by selecting specific parents for grouping. The parents are bulblet flowering and fruit variety group iris Netherlands, and the commodity sphere circumference diameter is 6-8cm; the chromosome number of the somatic cells is even;
(2) Planting parents: soaking parent seed balls in a carbendazim aqueous solution for half an hour in middle and late 11 months, taking out, washing with clear water for 2-3 times, planting the seed balls according to a plant row spacing of 20X 20cm, covering soil with a depth of 5-7cm, and watering thoroughly after planting;
(3) Hybridization: when the flowers just bloom and the stamens are not scattered, removing the drooping petals, the flag petals and the stamens of the plants serving as the female parent, and only leaving three eclosion stigmas and bagging; carefully taking off stamens of a plant used as a male parent by using tweezers when the stamens of the plant is mature and loose, then uniformly smearing pollen on the column heads of the female parent, and bagging and isolating the female parent after pollination is finished; repeating hybridization for 2-3 days to increase success rate, and taking out the sulfuric acid paper when the stigma is in a water loss wilting state 5-7 days after the second hybridization;
(4) And (3) seed collection: harvesting the capsules produced by hybridization in the step (3) after becoming yellow and mature, putting the capsules in a nylon mesh bag, hanging the nylon mesh bag in a ventilation position for drying, carefully pulling the capsules after drying to take out seeds, and selecting full seeds to be placed in a fine nylon bag and hanging the nylon mesh bag in a drying position for later use;
(5) Sowing: soaking the iris seed treated in the step (4) for 30min by using a thousandth of carbendazim aqueous solution, and then washing away carbendazim powder on the surface of the seed by using clear water for 2-3 times; placing the washed iris Netherlands seeds on wet filter paper, culturing at room temperature for about 2 days, sowing in yellow sand after the seeds are saturated with water, and culturing in a climatic chamber until seedlings emerge.
Optionally, 7-8 months before planting the seed balls in the step (2), spreading dazomet (25 kg/mu), sheep manure (70 kg/mu) and compound fertilizer (20 kg/mu) on the soil surface, deeply spinning the soil by using a rotary cultivator for 5-10cm, and covering the soil after rotary tillage by using a plastic film for 15-20 days.
Optionally, all operations in step (3) are performed on sunny days at 9-10 am.
Optionally, in the step (5), the water-saturated iris seed is sown in yellow sand as follows:
A layer of plastic woven bag is filled at the bottom of the plastic basin, then yellow sand with the thickness of 5-7cm is put in, the iris seeds of the Netherlands are spread on the yellow sand in a dispersed manner, and then the yellow sand with the thickness of 2-3cm is covered.
Further, the culture conditions of the artificial climate box in the step (5) are as follows:
The illumination time is 16h/d, the temperature is 23+/-1 ℃ during illumination, the temperature is 18+/-1 ℃ during darkness, and the illumination intensity is 20000-25000Lux.
Compared with the prior art, the invention has the following beneficial effects:
The invention selects a specific variety as a hybridization parent and performs field planting; when the flowers are just opened and are not scattered, performing matched hybridization pollination, and repeating hybridization pollination after 2-3 days; harvesting and drying in a ventilation place after capsules produced by hybridization are changed into yellow and mature; selecting full seeds, sowing the seeds in a basin filled with yellow sand, and culturing the seeds in a climatic chamber until the seeds germinate, wherein the culturing conditions are as follows: the illumination time is 16h/d, the temperature is 23+/-1 ℃ during illumination, the temperature is 18+/-1 ℃ during darkness, and the illumination intensity is 20000-25000Lux. The method can lead the hybrid setting rate of the iris lilacina to be more than 95 percent and the seed germination rate to be more than 10 percent, overcomes the traditional cognitive defect that the iris lilacina planted in China is not set, and has important milestone significance for promoting the breeding process of the iris lilacina in China and promoting the development of the industry.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only embodiments of the present invention, and that other drawings can be obtained according to the provided drawings without inventive effort for a person skilled in the art.
FIG. 1 is a photograph of a hybrid fruit capsule of Iris Netherlands.
FIG. 2 is a photograph of Iris Netherlands seed.
FIG. 3 is a photograph of germination of iris Netherlands seeds after sowing; wherein the arrow indicates the germinating seedling.
Detailed Description
The following will clearly and completely describe the technical solutions in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The word "embodiment" as used herein does not necessarily mean that any embodiment described as "exemplary" is preferred or advantageous over other embodiments. Performance index testing in the examples of the present application, unless otherwise specified, was performed using conventional testing methods in the art. It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the disclosure.
Unless otherwise defined, technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs; other test methods and techniques not specifically mentioned in the present application are those commonly used by those skilled in the art.
In the description of the present invention, it should be understood that the terms "medium," "upper," "lower," "ascending," "descending," "vertical," "face," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the present invention and simplify the description, and do not indicate or imply that the apparatus or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.
Numerous specific details are set forth in the following examples in order to provide a better understanding of the present application. It will be understood by those skilled in the art that the present application may be practiced without some of these specific details. In the examples, some methods, means, instruments, devices, etc. well known to those skilled in the art are not described in detail in order to highlight the gist of the present application.
On the premise of no conflict, the technical features disclosed by the embodiment of the application can be combined at will, and the obtained technical scheme belongs to the disclosure of the embodiment of the application.
The iris fruit in the following examples was collected from iris resource nursery of the academy of sciences of China, jiangsu province, and the test tools were all obtained from conventional products commercially available or commonly used in laboratories.
Example 1:
(1) Selection of hybrid parents: the hybrid parents are Red Ember and Tiger Eye, and the configured combination is Red Ember×Tiger Eye;
(2) Planting parents: the parent seed balls are planted 7 months and 20 days before planting, cotton-wool (25 kg/mu), sheep manure (70 kg/mu) and compound fertilizer (20 kg/mu) are scattered on the soil surface, the soil is deeply rotated by a rotary cultivator for 7cm, and the soil after rotary tillage is covered by a plastic film for 15 days. Soaking parent seed balls in carbendazim water solution for half an hour for 11 months and 20 days, taking out, washing with clear water for 2-3 times, planting in the ground according to plant row spacing of 20X 20cm, covering soil with depth of 5-7cm, and watering thoroughly after planting;
(3) Hybridization: when the flowers just bloom and are not scattered, removing the drooping petals, the flag petals and the stamens of the plants serving as the female parent, and only leaving three eclosion stigmas and bagging; when the plant stamen used as the male parent is mature and loose powder, carefully taking off the stamen by using tweezers, then uniformly smearing pollen on the female parent column heads, and bagging and isolating the hybridized female parent; repeating hybridization for 2-3 days to increase success rate, and taking out the sulfuric acid paper when the stigma is in a water loss wilting state 5-7 days after the second hybridization;
(4) And (3) seed collection: harvesting the capsules produced by hybridization after the capsules become yellow and mature, putting the capsules into a nylon mesh bag, hanging the nylon mesh bag at a ventilation position for drying, carefully pulling the capsules after drying to take out seeds, and selecting the filled seeds to be put into a fine nylon bag and hanging the nylon mesh bag at a drying position for later use;
(5) Sowing: soaking the hybrid seeds of the iris tectorum subjected to the treatment in the step (4) for 30min by using a thousandth of carbendazim aqueous solution, and then washing off carbendazim powder on the surfaces of the seeds by using clear water for 2-3 times; placing the washed iris seed on wet filter paper, culturing at room temperature for 2 days, sowing in a plastic basin after the seed is saturated with water, filling a layer of plastic woven bag at the bottom of the plastic basin, then placing yellow sand with the thickness of 5-7cm, finally dispersing and spreading the iris seed on the yellow sand, and covering the yellow sand with 2-3cm; the sown iris seed is watered thoroughly and then put into an artificial climatic chamber for culture, and the culture conditions are as follows: the illumination time is 16h/d, the temperature is 23+/-1 ℃ during illumination, the temperature is 18+/-1 ℃ during darkness, and the illumination intensity is 20000-25000Lux until seedlings emerge.
The calculated Red EmberxTiger Eye hybridization seed setting rate is 100%, and the hybridization seed sowing emergence rate is 10.2%.
Example 2:
Similar to the procedure of example 1, the difference is that: the hybridization combination in step (1) is Tiger eye×Red Ember.
The calculated hybridization seed setting rate of Tiger Eye x Red Ember is 95.6 percent, and the sowing emergence rate of the hybridization seeds is 11.4 percent.
Example 3:
Similar to the procedure of example 1, the difference is that: the hybrid parents in the step (1) are Picasso and Red Ember, and the configured hybrid combination is Picasso×Red Ember.
Calculated Picasso×Red Ember hybrid seed setting rate is 98.4%, hybrid seed sowing emergence rate is 12.5%.
Comparative example 1:
Similar to the procedure of example 1, the difference is that: the hybrid parent in the step (1) selects the Netherlands iris, nofa Blue and Nofa White of the large bulb flowering group, and the commodity sphere circumference diameters of the two are all more than 10cm. The hybridization combination was Nofa Blue × Nofa White.
The hybridization seed setting rate was calculated to be Nofa Blue × Nofa White to be 0%.
Comparative example 2:
Similar to the procedure of example 1, the difference is that: selection of hybrid parents in step (1): one parent is the lotus iris Nofa Blue of the large bulb flowering group, the other parent is the lotus iris Red number of the small bulb flowering group, and the configured hybridization combination is Nofa Blue ×red number.
The calculated Nofa Blue ×Red Ember hybridization seed setting rate was 0%.
Comparative example 3:
similar to the procedure of example 1, the difference is that: selection of hybrid parents in step (1): one parent is the lotus iris Nofa Blue of the large bulb flowering group, the other parent is the lotus iris Red number of the small bulb flowering group, and the configured hybridization combination is Red number multiplied by Nofa Blue.
The hybridization seed setting rate was calculated to be 0% for Red Ember X Nofa Blue.
The invention belongs to the first report in China that seeds can be obtained by hybridization of iris liliflora, and the selection of related hybrid parents is the key for obtaining the seeds of iris liliflora.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (6)
1. A method for obtaining hybrid seeds and seed germination of iris Netherlands, the method comprising the steps of:
(1) Selection of hybrid parents: selecting iris Netherlands of the bulblet flowering product species group as a parent; the hybrid parents are Red Ember and Tiger Eye or the hybrid parents are Picasso and Red Ember;
(2) Planting parents: soaking the parent seed balls in a carbendazim aqueous solution for half an hour in middle and late 11 months, taking out, washing with clear water for 2-3 times, planting the seed balls according to a plant row spacing of 20X 20 cm, covering soil with a depth of 5-7 cm, and watering thoroughly after planting;
(3) Hybridization: when the flowers are just opened and not scattered, removing the drooping petals, the flag petals and the stamens of the plants serving as the female parent, and only leaving three eclosion stigmas and bagging; carefully taking off stamens with forceps when the stamens of the plant used as the male parent is mature and scattered, then uniformly smearing pollen on the column heads of the female parent, and bagging and isolating the hybridized female parent; repeating hybridization after 2-3 days; 5-7 days after the second hybridization, taking off the sulfuric acid paper of the bagging when the stigma is in a water loss wilting state;
(4) And (3) seed collection: collecting capsules produced by hybridization in the step (3) after becoming yellow and mature into a laboratory, placing the capsules in a nylon mesh bag, hanging the nylon mesh bag at a ventilation position for drying, carefully pulling the capsules after drying to take out seeds, selecting full seeds, and placing the full seeds in a fine nylon bag and hanging the nylon bags at a drying position for later use;
(5) Sowing: soaking the iris seed 30 min treated in the step (4) by using a thousandth of carbendazim aqueous solution, washing off carbendazim powder on the surface of the seed by using clear water, and placing the powder on wet filter paper for culture at room temperature; sowing the water-saturated iris seeds in yellow sand, and culturing in a climatic chamber until seedlings emerge.
2. The method for obtaining hybrid seeds and germinating seeds of iris Netherlands according to claim 1, wherein the commercial sphere diameter of the parent iris Netherlands of the bulblet in the step (1) is 6-8 cm and the chromosome number of the somatic cells is even.
3. The method for obtaining hybrid seeds and germinating seeds of iris Netherlands according to claim 1, wherein in the step (2), before planting the seeds in 7-8 months, cotton-wool, sheep manure and compound fertilizer are spread on the soil surface and the soil is deeply spun by a rotary cultivator for 5-10 cm, and the soil after rotary tillage is covered by a plastic film for 15-20 days.
4. The method for obtaining hybrid seeds and germinating seeds of iris Netherlands according to claim 1, wherein the hybridization operation in the step (3) is performed on sunny days at 9-10 am.
5. The method for obtaining hybrid seeds and germinating seeds of iris Netherlands according to claim 1, wherein in the step (5), the water-saturated iris Netherlands seeds are sown in yellow sand as follows:
a layer of plastic woven bag is filled at the bottom of the plastic basin, then yellow sand with the thickness of 5-7 cm is put in, the iris seeds are spread on the yellow sand in a dispersed manner, and then the yellow sand with the thickness of 2-3 cm is covered.
6. The method for obtaining hybrid seeds and seed germination of iris Netherlands according to claim 1 or 5, wherein the culture conditions of the artificial climatic chamber in step (5) are: the illumination time is 16 h/d, the temperature is 23+/-1 ℃ during illumination, the temperature is 18+/-1 ℃ during darkness, and the illumination intensity is 20000-25000 Lux.
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| CN109964810A (en) * | 2019-04-24 | 2019-07-05 | 江苏里下河地区农业科学研究所 | A method of obtaining pale flag and Iris germanica distant hybrid |
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| CN101011024A (en) * | 2007-02-16 | 2007-08-08 | 北京林业大学 | Novel crossbreeding method for iris |
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| CN109182375A (en) * | 2018-09-26 | 2019-01-11 | 江苏省中国科学院植物研究所 | A kind of genetic transforming method of Iris germanica |
| CN109964810A (en) * | 2019-04-24 | 2019-07-05 | 江苏里下河地区农业科学研究所 | A method of obtaining pale flag and Iris germanica distant hybrid |
| CN112299810A (en) * | 2019-07-25 | 2021-02-02 | 孔德奎 | Assembled building heat-insulation wallboard and preparation method thereof |
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