CN116929874A - Tissue slice specimen manufacturing device - Google Patents
Tissue slice specimen manufacturing device Download PDFInfo
- Publication number
- CN116929874A CN116929874A CN202310814860.8A CN202310814860A CN116929874A CN 116929874 A CN116929874 A CN 116929874A CN 202310814860 A CN202310814860 A CN 202310814860A CN 116929874 A CN116929874 A CN 116929874A
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- box body
- tissue slice
- upper box
- plate
- spreading plate
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- 238000004519 manufacturing process Methods 0.000 title abstract description 11
- 238000003892 spreading Methods 0.000 claims abstract description 54
- 230000007480 spreading Effects 0.000 claims abstract description 50
- 238000002360 preparation method Methods 0.000 claims abstract description 31
- 238000010438 heat treatment Methods 0.000 claims abstract description 15
- 230000007246 mechanism Effects 0.000 claims abstract description 5
- 238000003825 pressing Methods 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 8
- 239000004809 Teflon Substances 0.000 claims description 4
- 229920006362 Teflon® Polymers 0.000 claims description 4
- 239000011248 coating agent Substances 0.000 claims description 4
- 238000000576 coating method Methods 0.000 claims description 4
- 229910052742 iron Inorganic materials 0.000 claims description 4
- 239000002184 metal Substances 0.000 claims description 3
- 229910052751 metal Inorganic materials 0.000 claims description 3
- -1 polytetrafluoroethylene Polymers 0.000 claims description 3
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 3
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 2
- 239000000427 antigen Substances 0.000 description 12
- 102000036639 antigens Human genes 0.000 description 12
- 108091007433 antigens Proteins 0.000 description 12
- 239000003153 chemical reaction reagent Substances 0.000 description 8
- 238000011534 incubation Methods 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 238000007789 sealing Methods 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 230000008439 repair process Effects 0.000 description 4
- 230000036571 hydration Effects 0.000 description 3
- 238000006703 hydration reaction Methods 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000007797 corrosion Effects 0.000 description 2
- 238000005260 corrosion Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 230000001575 pathological effect Effects 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 231100000027 toxicology Toxicity 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 239000000969 carrier Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000000887 hydrating effect Effects 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 238000011206 morphological examination Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/44—Sample treatment involving radiation, e.g. heat
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a tissue slice specimen preparation device, which relates to the technical field of specimen preparation and comprises the following components: the lower box body is cuboid and has no top plate; the upper box body is cuboid and has no top plate and bottom plate, and can be placed above the lower box body; the sheet spreading plate is arranged in the upper box body, an upper flange is arranged on the first edge of the sheet spreading plate, and a lower flange is arranged on the second edge of the sheet spreading plate; the piece spreading plate is fixedly connected with a rotating shaft which is in rotating fit with the upper box body; the upper flange and the lower flange are parallel to the rotating shaft; one end of the rotating shaft is fixedly provided with an adjusting knob, and the other end of the rotating shaft is fixedly provided with a gear; the locking mechanism comprises a locking plate, a first fixing bolt, a second fixing bolt and a torsion spring; and the self-heating patch is adhered to the bottom surface of the spreading plate. The tissue slice specimen manufacturing device does not need a power supply, is convenient to use, is easy to manufacture and has low cost.
Description
Technical Field
The invention relates to the technical field of specimen preparation, in particular to a tissue slice specimen preparation device.
Background
In the toxicology, biomedical fields, morphological examination or research of animal tissues or organs is often required to determine the effect of toxicants or diseases on the body, all depending on the well-made tissue slice specimens.
Currently, tissue slice specimen preparation is heavily dependent on pathology specialized equipment such as: slide-baking machines, staining machines, incubators, etc., and specimen preparation would be difficult if these specialized equipment were missing or were not powered. However, in most toxicology or biomedical laboratories, such specialized equipment is often lacking, resulting in inconvenient tissue slice preparation.
In addition, there are many repetitive operations in the specimen preparation process, such as: when the slices are baked by draining, the tissue slice specimens need to be placed on a slice baking machine, when dewaxing and hydration are carried out, the tissue slice specimens need to be vertically placed into a slide frame one by one, when antigens are closed, the tissue slice specimens need to be taken out of the slide frame one by one and horizontally placed on a slice spreading plate, when washing is needed, the tissue slice specimens need to be vertically placed into the slide frame, when incubation is needed, the tissue slice specimens need to be horizontally placed on the slice spreading plate, and the like, so the repeated operation is repeated for a plurality of times, and the operation is very complicated.
Disclosure of Invention
The invention aims to provide a tissue slice specimen preparation device which solves the problems in the prior art and improves the convenience of tissue slice preparation.
In order to achieve the above object, the present invention provides the following solutions:
the present invention provides a tissue slice specimen preparation device, comprising:
the lower box body is cuboid and has no top plate;
the upper box body is cuboid and has no top plate and bottom plate, the upper box body can be placed above the lower box body, and the bottom side wall of the upper box body can be abutted with the top end of the lower box body;
the spreading plate is arranged in the upper box body, an upper flange is arranged on the first edge of the spreading plate, a lower flange is arranged on the second edge of the spreading plate, a water leakage groove is arranged on the lower flange, and the upper flange is opposite to the lower flange; the piece spreading plate is fixedly connected with a rotating shaft, and the rotating shaft is in running fit with the upper box body; the upper flange and the lower flange are parallel to the rotating shaft; one end of the rotating shaft is fixedly provided with an adjusting knob, and the other end of the rotating shaft is fixedly provided with a gear; the adjusting knob and the gear are both positioned outside the upper box body;
the locking mechanism comprises a locking plate, a first fixing bolt, a second fixing bolt and a torsion spring, wherein the first fixing bolt and the second fixing bolt are respectively and fixedly installed on the side wall of the upper box body, the middle part of the locking plate and the torsion spring are rotationally sleeved on the second fixing bolt, one end of the torsion spring is abutted with the locking plate, the other end of the torsion spring is abutted with the first fixing bolt, one end of the locking plate is provided with a tooth hook, the other end of the locking plate is provided with a handle, and the tooth hook can be inserted between two adjacent teeth of the gear; the torsion spring is used for enabling the tooth hook to be in close contact with the gear;
and the self-heating patch is adhered to the bottom surface of the sheet spreading plate.
Preferably, the box further comprises a top cover, wherein one end of the top cover is hinged with the top end of the upper box body through a hinge.
Preferably, the top cover is made of polytetrafluoroethylene, the upper box body and the lower box body are made of metal, and the spreading plate is made of iron.
Preferably, the tissue slice processing device further comprises a magnet pressing bar, wherein the magnet pressing bar is in a strip shape and is used for pressing a tissue slice specimen, and the tissue slice specimen is located between the spreading plate and the magnet pressing bar.
Preferably, the inner wall of the upper box body and the inner wall of the lower box body are both provided with a teflon coating.
Preferably, the bottom of the upper box body is gradually narrowed, and when the upper box body is placed above the lower box body, the bottom end of the upper box body stretches into the lower box body.
Preferably, the number of the lower box bodies is multiple.
Preferably, the end face, far away from the upper box body, of the gear is provided with an inclination indication arrow, and the side wall of the upper box body is provided with inclination scales distributed along the circumferential direction of the gear.
Compared with the prior art, the invention has the following technical effects:
the tissue slice specimen manufacturing device does not need a power supply, is convenient to use, is easy to manufacture and has low cost.
The tissue slice specimen manufacturing device provided by the invention adopts the self-heating patch to provide a heat supply source, and utilizes the reaction of the polymer in the patch and oxygen in the air to continuously generate heat for 12 hours, so that the tissue slice specimen manufacturing device is low in price, easy to purchase and stable in quality, and can solve the problem of dependence of steps such as slice baking, dewaxing, incubation and the like on electric power.
The tissue slice specimen preparation device adopts a split design of the slice spreading plate with the adjustable inclination angle and the upper box body and the lower box body, can finish the operation processes of draining and baking slices, dewaxing and hydrating, antigen sealing, antibody incubation and the like under the condition of no professional pathological equipment and equipment such as a slice spreading and baking machine, an incubator, a slice dyeing machine, a glass slide frame and the like, and brings great convenience for carrying out histomorphometric examination or research in a non-pathological professional laboratory.
The tissue slice sample preparation device adopts the structures of the knob, the rotating shaft, the gear and the like, can rapidly realize the switching of the tissue slice sample from the horizontal position to the vertical position, and avoids the use of carriers such as a slide frame and the like, thereby avoiding repeated operations such as repeated loading, placing and the like of the tissue slice sample and improving the efficiency.
The tissue slice sample preparation device is provided with a plurality of lower box bodies, can be filled with a plurality of reagents such as a dewaxing agent, ethanol with different concentration gradients, distilled water and the like, and the upper box bodies can be quickly connected with or separated from the lower box bodies, so that the tissue slice samples can be conveniently soaked in various reagents. Simultaneously, repeatedly rotating the tilt angle adjusting knob of the spreading plate along the clockwise or anticlockwise direction can make the tissue slice specimen swing back and forth in the reagent solution and stir the liquid, so that the specimen is more fully contacted with the reagent.
The tissue slice specimen preparation device is made of metal materials, the lower box body can be directly heated to repair antigens after being filled with antigen repair liquid, and the tissue slice specimens do not need to be filled into a special pot or an antigen repair instrument for repair.
The device for preparing the tissue slice sample adopts the magnet pressing strip to fix the tissue slice sample, and is simple and convenient.
The structure of the tissue slice specimen manufacturing device adopting the gear and the locking plate can flexibly adjust the inclination angle of the spreading plate, which is beneficial to draining of the tissue slice specimen and prevents the tissue slice from sliding downwards and separating from the glass slide.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions of the prior art, the drawings that are needed in the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic view of a tissue slice specimen preparation apparatus according to the present invention;
FIG. 2 is a front view of a tissue slice specimen preparation device of the present invention;
FIG. 3 is a top view of a tissue slice specimen preparation device of the present invention;
FIG. 4 is a left side view of the tissue slice specimen preparation device of the present invention;
FIG. 5 is a schematic view showing a part of the structure of a tissue slice specimen preparing apparatus according to the present invention;
FIG. 6 is a schematic view of a portion of a tissue slice specimen preparation apparatus according to the present invention;
wherein, 1, top cap; 2. a hinge; 3. an upper flange; 4. a spreading plate; 5. an adjustment knob; 6. a lower flange; 7. an upper case; 8. a lower case; 9. a gear; 10. tissue slice specimens; 11. a magnet pressing bar; 12. a button loop; 13. a rotating shaft; 14. self-heating patches; 15. a water leakage groove; 16. inclination indication arrow; 17. an inclination scale; 18. a torsion spring; 19. a first fixing bolt; 20. a second fixing bolt; 21. and (5) locking plates.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention aims to provide a tissue slice specimen preparation device which solves the problems in the prior art and improves the convenience of tissue slice preparation.
In order that the above-recited objects, features and advantages of the present invention will become more readily apparent, a more particular description of the invention will be rendered by reference to the appended drawings and appended detailed description.
As shown in fig. 1 to 6, the present embodiment provides a tissue slice specimen preparing apparatus comprising:
the lower box body 8 is cuboid, and has no top plate, namely the top end of the lower box body 8 is open;
the upper box body 7 is cuboid, no top plate and no bottom plate are arranged on the upper box body 7, the upper box body 7 can be placed above the lower box body 8, and the bottom side wall of the upper box body 7 can be abutted with the top end of the lower box body 8;
the spreading plate 4, the spreading plate 4 is arranged in the upper box body 7, the first edge of the spreading plate 4 is provided with an upper flange 3, the second edge of the spreading plate 4 is provided with a lower flange 6, the upper flange 3 is opposite to the lower flange 6, and the lower flange 6 is provided with a water leakage groove 15; the piece spreading plate 4 is fixedly connected with a rotating shaft 13, and the rotating shaft 13 is in running fit with the side wall of the upper box body 7 through a bearing; the upper flange 3 and the lower flange 6 are parallel to the rotating shaft 13; one end of the rotating shaft 13 is fixedly provided with an adjusting knob 5, and the other end is fixedly provided with a gear 9; the adjusting knob 5 and the gear 9 are both positioned outside the upper box body 7; the inclination angle of the spreading plate 4 can be adjusted by rotating the distance adjusting knob;
the locking mechanism comprises a locking plate 21, a first fixing bolt 19, a second fixing bolt 20 and a torsion spring 18, wherein the first fixing bolt 19 and the second fixing bolt 20 are respectively and fixedly arranged on the side wall of the upper box body 7, the middle part of the locking plate 21 and the torsion spring 18 are both rotationally sleeved on the second fixing bolt 20, one end of the torsion spring 18 is abutted with the locking plate 21, the other end of the torsion spring is abutted with the first fixing bolt 19, one end of the locking plate 21 is provided with a tooth hook, the other end of the locking plate is provided with a handle, and the tooth hook can be inserted between two adjacent teeth of the gear 9; the torsion spring 18 is used for tightly contacting the tooth hook with the gear 9;
the self-heating patch 14, the self-heating patch 14 is adhered to the bottom surface of the sheet spreading plate 4; the self-heating patch 14 is an existing mature product, which is directly commercially available. The self-heating patch 14 is adopted to provide a heat source, and the polymer in the self-heating patch 14 is utilized to react with oxygen in the air to continuously generate heat for 12 hours, so that the self-heating patch is low in price, easy to purchase and stable in quality, and the problem of dependence of the steps of baking, dewaxing, incubation and the like on electric power can be solved.
Referring to fig. 6, the locking mechanism operates as follows: under the torsion of the torsion spring 18, the tooth hook on the locking plate 21 can be tightly inserted between two teeth of the gear 9, so that the sheet spreading plate 4 is fixed at a certain inclination angle. When the piece-spreading plate 4 needs to be rotated, the handle part at the lower end of the locking plate 21 can be pulled along the direction of a horizontal arrow, the tooth hook part at the upper end of the locking plate 21 can rotate anticlockwise, the gear 9 of the rotating shaft 13 is loosened, at the moment, the inclination angle of the piece-spreading plate 4 can be adjusted by rotating the inclination angle adjusting knob 5 of the piece-spreading plate 4, the handle part of the locking plate 21 is loosened, and the tooth hook at the upper end of the locking plate 21 can be reinserted into the gear teeth of the gear 9, so that the inclination angle of the piece-spreading plate 4 can be locked.
In this embodiment, the end surface of the gear 9 away from the upper case 7 is provided with an inclination indication arrow 16, and the side wall of the upper case 7 is provided with inclination scales 17 distributed along the circumferential direction of the gear 9. The inclination scale 17 pointed by the indication arrow is the inclination of the sheet spreading plate 4, so that the inclination of the sheet spreading plate 4 can be intuitively known and conveniently controlled. The structure of the gear 9 and the locking plate 21 can flexibly adjust the inclination angle of the spreading plate 4, which is beneficial to draining the tissue slice specimen 10 and prevents the tissue slice from sliding downwards and separating from the glass slide.
The tissue slice specimen manufacturing device of the embodiment further comprises a top cover 1, wherein one end of the top cover 1 is hinged with the top end of the upper box body 7 through a hinge 2; the top cover 1 is made of heat-resistant and corrosion-resistant polytetrafluoroethylene, heat and steam can be radiated after the top cover 1 is opened, heat can be preserved in a dark place after the top cover 1 is closed, and the top cover 1 is provided with a buckle 12, wherein the buckle 12 is used for being connected with the upper box body 7.
The upper box body 7 and the lower box body 8 are made of metal, and the spreading plate 4 is made of iron. The inner wall of the upper box body 7 and the inner wall of the lower box body 8 are both provided with a Teflon coating, and the Teflon coating has the functions of heat resistance and corrosion resistance. After the lower box body 8 is filled with the antigen retrieval liquid, the antigen can be directly heated to retrieve the antigen, and the tissue slice specimen 10 does not need to be filled into a special pot or an antigen retrieval instrument for retrieval.
The tissue slice sample manufacturing device of the embodiment further comprises a magnet pressing strip 11, the magnet pressing strip 11 is in a strip shape, the magnet pressing strip 11 is used for pressing the tissue slice sample 10, and the tissue slice sample 10 is located between the spreading plate 4 and the magnet pressing strip 11. Because the magnet pressing strip 11 is attracted with the iron spreading plate 4, when the magnet pressing strip 11 is pressed above the tissue slice specimen 10, the tissue slice specimen 10 can be fixed, and the tissue slice specimen 10 is prevented from falling off in the rotation process of the spreading plate 4, so that the method is simple and convenient.
In this embodiment, the bottom of the upper box body 7 is gradually narrowed, and when the upper box body 7 is placed above the lower box body 8, the bottom end of the upper box body 7 extends into the lower box body 8, so that the liquid can be prevented from overflowing from the joint of the lower box body 8 and the upper box body 7 to a certain extent. When cleaning is needed, the upper box body 7 can be conveniently pulled out from the lower box body 8.
In this embodiment, the number of the lower cases 8 is plural; the different lower box bodies 8 can be filled with a plurality of reagents such as dewaxing agents, ethanol with different concentration gradients, distilled water and the like, the upper box body 7 is filled with a tissue slice specimen 10, and the tissue slice specimen can be quickly connected with or separated from the lower box body 8, so that the soaking treatment of the tissue slice specimen 10 in various reagents can be conveniently realized. Simultaneously, repeatedly rotating the tilt adjusting knob 5 of the spreading plate 4 along the clockwise or anticlockwise direction can make the tissue slice specimen 10 swing back and forth in the reagent solution, agitate the liquid, and make the specimen contact with the reagent more fully.
The specific method of using the tissue slice sample preparation device of the present embodiment is as follows:
when the baking sheet is drained, water drops drained from the slide glass in the upper box body 7 can drop into the lower box body 8 through the water leakage groove 15, and the lower box body 8 is separated, so that water can be poured out or the self-heating patch 14 can be replaced;
when dewaxing hydration is needed, the self-heating patch 14 on the back of the spreading plate 4 can be uncovered, the magnet pressing bar 11 is used for pressing the tissue slice specimen 10, the lower box body 8 is filled with a dewaxing agent, the locking plate 21 is loosened, the inclination angle adjusting knob 5 of the spreading plate 4 is rotated anticlockwise, the inclination angle is adjusted to 90 degrees, and the baked tissue slice specimen 10 can be immersed in the dewaxing agent or water to finish dewaxing hydration;
when antigen sealing or antibody incubation is needed, a small amount of water can be added into the lower box body 8 to keep humidity, a self-heating patch 14 with two thirds width is stuck on the back surface of the spreading plate 4, the locking plate 21 is loosened, the inclination angle adjusting knob 5 to 0 degree of the spreading plate 4 is rotated clockwise, the spreading plate 4 is kept horizontal, antigen sealing liquid or antibody is dripped onto the tissue slice specimen 10, and the top cover 1 is covered, so that antigen sealing or antibody incubation can be started; after the completion, the self-heating patch 14 can be removed, then the tilt angle of the spreading plate 4 is adjusted to 90 degrees by using the tilt angle adjusting knob 5 of the spreading plate 4, so that the tissue slice is vertically immersed into the detergent in the lower box body 8 for washing, and after color development and counterstaining and dehydration, the tilt angle of the spreading plate 4 is adjusted to 0 degree, and then the neutral resin sealing piece can be dripped.
In the description of the present invention, it should be noted that the positional or positional relationship indicated by the terms "top", "bottom", "inner", "outer", etc. are based on the positional or positional relationship shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the apparatus or element in question must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
The principles and embodiments of the present invention have been described in detail with reference to specific examples, which are provided to facilitate understanding of the method and core ideas of the present invention; also, it is within the scope of the present invention to be modified by those of ordinary skill in the art in light of the present teachings. In view of the foregoing, this description should not be construed as limiting the invention.
Claims (8)
1. A tissue slice specimen preparation device, comprising:
the lower box body is cuboid and has no top plate;
the upper box body is cuboid and has no top plate and bottom plate, the upper box body can be placed above the lower box body, and the bottom side wall of the upper box body can be abutted with the top end of the lower box body;
the spreading plate is arranged in the upper box body, an upper flange is arranged on the first edge of the spreading plate, a lower flange is arranged on the second edge of the spreading plate, a water leakage groove is arranged on the lower flange, and the upper flange is opposite to the lower flange; the piece spreading plate is fixedly connected with a rotating shaft, and the rotating shaft is in running fit with the upper box body; the upper flange and the lower flange are parallel to the rotating shaft; one end of the rotating shaft is fixedly provided with an adjusting knob, and the other end of the rotating shaft is fixedly provided with a gear; the adjusting knob and the gear are both positioned outside the upper box body;
the locking mechanism comprises a locking plate, a first fixing bolt, a second fixing bolt and a torsion spring, wherein the first fixing bolt and the second fixing bolt are respectively and fixedly installed on the side wall of the upper box body, the middle part of the locking plate and the torsion spring are rotationally sleeved on the second fixing bolt, one end of the torsion spring is abutted with the locking plate, the other end of the torsion spring is abutted with the first fixing bolt, one end of the locking plate is provided with a tooth hook, the other end of the locking plate is provided with a handle, and the tooth hook can be inserted between two adjacent teeth of the gear; the torsion spring is used for enabling the tooth hook to be in close contact with the gear;
and the self-heating patch is adhered to the bottom surface of the sheet spreading plate.
2. The tissue slice specimen preparation device according to claim 1, wherein: still include the top cap, top cap one end pass through the hinge with the top of last box body articulates.
3. The tissue slice specimen preparation device according to claim 2, wherein: the top cover is made of polytetrafluoroethylene, the upper box body and the lower box body are made of metal, and the spreading plate is made of iron.
4. The tissue slice specimen preparation device according to claim 3, wherein: the tissue slice processing device further comprises a magnet pressing strip, wherein the magnet pressing strip is in a strip shape and is used for pressing a tissue slice specimen, and the tissue slice specimen is located between the slice spreading plate and the magnet pressing strip.
5. The tissue slice specimen preparation device according to claim 3, wherein: the inner wall of the upper box body and the inner wall of the lower box body are both provided with a Teflon coating.
6. The tissue slice specimen preparation device according to claim 1, wherein: the bottom of the upper box body is gradually narrowed, and when the upper box body is placed above the lower box body, the bottom end of the upper box body stretches into the lower box body.
7. The tissue slice specimen preparation device according to claim 1, wherein: the number of the lower box bodies is multiple.
8. The tissue slice specimen preparation device according to claim 1, wherein: the gear is far away from the inclination indication arrow is arranged on the end face of the upper box body, and inclination scales distributed along the circumferential direction of the gear are arranged on the side wall of the upper box body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310814860.8A CN116929874A (en) | 2023-07-04 | 2023-07-04 | Tissue slice specimen manufacturing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310814860.8A CN116929874A (en) | 2023-07-04 | 2023-07-04 | Tissue slice specimen manufacturing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116929874A true CN116929874A (en) | 2023-10-24 |
Family
ID=88387192
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310814860.8A Pending CN116929874A (en) | 2023-07-04 | 2023-07-04 | Tissue slice specimen manufacturing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116929874A (en) |
-
2023
- 2023-07-04 CN CN202310814860.8A patent/CN116929874A/en active Pending
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