CN116897866A - Indoor culture method for young largemouth black bass and application of method in strengthening liver function of young largemouth black bass - Google Patents
Indoor culture method for young largemouth black bass and application of method in strengthening liver function of young largemouth black bass Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/13—Prevention or treatment of fish diseases
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
- A01K63/042—Introducing gases into the water, e.g. aerators, air pumps
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
- A01K63/045—Filters for aquaria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F7/00—Aeration of stretches of water
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention provides an indoor culture method of young largemouth bass and application thereof in reinforcing liver function of young largemouth bass, wherein the method mainly comprises the following steps: 1) Preparing a circulating water culture system; 2) Disinfecting juvenile fish and putting the juvenile fish in a pool; 3) Feeding and daily management. The invention carries out scientific indoor young larch cultivation by combining a circulating water cultivation system, the use of anti-stress dynamic protection products and a proper feeding strategy on the premise of defining stress sources, nutrition requirements of young larch and metabolic characteristics of the larch in the indoor cultivation process of young larch. The method can reduce stress reaction in the indoor culture process of the young micropterus salmoides, strengthen liver health condition of the young micropterus salmoides, strengthen liver function of the young micropterus salmoides, improve disease resistance of the young micropterus salmoides, and realize indoor healthy culture of the young micropterus salmoides.
Description
Technical Field
The invention belongs to the field of young fish culture, relates to a healthy culture method of young largemouth black bass which is an important economic fish, and in particular relates to an indoor culture method of young largemouth black bass and application of the method in reinforcing liver functions of the young largemouth black bass.
Background
The largehead jewfish (Micropterus salmoides) belongs to the order of the weever (Perciformes), the sub-order of the weever (Percoides), the family of the acanthocephalidae (Centralchidae) and the genus of the jewfish (Micropterus), commonly known as California weever, is originally produced in North America, introduced in the year 70 of the 20 th century in China, and artificially bred in 1983. The micropterus salmoides have the advantages of strong disease resistance, rapid growth, tender and delicious meat quality, no intramuscular thorns and the like, and are favored by the Chinese people. In addition, with the development and improvement of artificial compound feed, the yield of the largehead jewfish in 2021 is over 50 ten thousand tons, so the largehead jewfish is known as the fifth big household fish in China.
However, there are still many problems in the culture process of the largemouth bass, especially the young fish is easily affected by the external environment, such as accumulation of toxic and harmful substances in water, large temperature change of the culture environment, low oxygen and the like, so as to induce stress. In addition, in addition to the stress-inducing factors, the liver of the young micropterus salmoides is sensitive to the composition and the nutrition level of nutrients, and the liver health of the young micropterus salmoides is easily damaged due to malnutrition and overnutrition in the feed. Up to now, the problems of stress and liver disease frequency (especially liver disease frequency induced by liver fat deposition) in the young perch breeding process seriously threatens the development of the breeding industry. Therefore, according to the characteristics of life habit, nutrition requirement and the like, research and development of an indoor cultivation method suitable for healthy cultivation of young largehead jersey are urgently needed, low-stress cultivation of the largehead jersey is achieved, liver functions of the largehead jersey are enhanced at the stage of young largehead jersey, disease resistance of the largehead jersey is improved, and accordingly healthy development of largehead jersey cultivation industry is promoted.
Disclosure of Invention
The invention provides an indoor culture method of young micropterus salmoides and application thereof in reinforcing liver functions of young micropterus salmoides, which provides proper culture water environment, balanced nutrition feed and healthy and scientific feeding management for the indoor culture of young micropterus salmoides, saves culture water and feed cost, and simultaneously provides direct technical support for the indoor culture of the liver functions of the young micropterus salmoides.
The technical scheme of the invention is that the indoor culture method of the young largemouth bass comprises the following steps:
s1, preparing an indoor circulating water culture system, storing water after cleaning and sterilizing, circulating, aerating and simultaneously carrying out water culture on garlic;
s2, disinfecting young largemouth bass by adopting saline solution, and then placing the young largemouth bass into a circulating water culture system in the S1, wherein the difference between the body temperature of the young largemouth bass and the water temperature in the system is less than 0.5 ℃ in the process of putting the young largemouth bass into the pond; the density of the juvenile fish is 8kg/m 3 The young fish is put into the pool and then anti-stress animal protection products are sprayed in the system;
s3, feeding feed after the young fish is put into the pond and fasted for 20-24 hours, cleaning the culture system in time, monitoring the water quality, controlling the water temperature of the water body to be 22-27 ℃, and maintaining the dissolved oxygen to be more than or equal to 7mg/L, pH to be 7-8 and the ammonia nitrogen to be less than 0.1 mg/L.
Further, the indoor circulating water culture system comprises a water storage aeration tank, a circulating tank, a culture tank, a water pump and corresponding water injection and outlet pipelines, wherein a step filtering facility is arranged in the circulating tank, a water outlet pipe of the circulating tank is connected to a water injection port of the culture tank, the water injection port of the culture tank is provided with a filtering facility, and a water outlet of the culture tank is connected to the water injection port of the circulating tank through a pipeline; the water outlet of the water storage aeration tank is connected to the water injection port of the circulating tank through a pipeline
Further, the water-cultured garlic is positioned in the circulating pool, when in water culture, the germinated garlic cloves are placed on a water-culture foam plate for fixation and float in the water body of the circulating pool for cultivation, and the density of the garlic cloves is 180-220 grains/m 2 And (5) controlling.
Further, the mass concentration of the saline water in the S2 is 3-5 mill, and the saline water is prepared by adopting aerated water in a circulating water culture system.
Further, the anti-stress animal protection product in S2 is Vc stress agent with the concentration of 28-32 g/m 3 The method comprises the steps of carrying out a first treatment on the surface of the It is configured in a circulation tank and is kept 1 time per week at the subsequent application frequency.
Further, in the juvenile fish feed in S3, the carbohydrate content is 10% or less, the fat content is 15% or less, and the protein content is 45% or more.
Further, when the feed is fed in the step S3, the feeding level is 80-85% of the apparent satiety feeding amount, and the feeding frequency is 2-3 times/day.
Further, food residues and excrement are timely discharged in the cultivation process in the step S; the feed which is not ingested within half an hour after feeding is timely discharged after feeding the young fish. A large amount of defecation is started after the juvenile fish ingests for 3 hours.
And (3) changing water of at least 1/5 of the water in the culture system every day, discharging the changed water out of the culture system, and supplementing water stored in the reservoir in advance and aerated for more than one day into a circulating pool of the circulating water culture system. When the water quality detection of the water body of the culture system does not meet the standard, the water exchange amount of the water body in the circulating water culture system is increased.
The invention also relates to application of the method in strengthening liver function of juvenile fish.
The invention has the following beneficial effects:
1. according to the invention, through the filtration and aeration effects of the circulating water culture system and the absorption effect of the water culture garlic on water pollutants, the culture water in the system can be effectively purified, the good water quality is ensured, the young cultured micropterus salmoides have good living environment, and the fish stress caused by the aggregation of the culture water pollutants is reduced. In addition, the purified water body is recycled, so that the consumption of the culture water body is greatly reduced.
2. By utilizing the sterilizing effect and the antioxidation effect of the garlic sprouts in the system, the invention can effectively enhance the antioxidation capability of the liver of the young micropterus salmoides and inhibit the growth of harmful bacteria in the culture water body while jointly filtering and aerating and purifying the culture water body, thereby playing the roles of enhancing the antioxidation capability of the liver of the young micropterus salmoides, reducing the risk of bacterial infection of the young micropterus salmoides and enhancing the disease resistance thereof.
3. The invention scientifically matches the effects of purifying water quality, resisting oxidation and sterilizing of the water-cultured garlic with the effects of resisting stress of Vc stress and enhancing disease resistance of young fishes, and the synergistic effect generated by the water-cultured garlic further reduces the stress reaction of young fishes of the cultured largehead bass, enhances the oxidation resistance of livers of the largehead bass and strengthens the liver functions of the largehead bass, so that death of the young fishes of the largehead bass caused by disease infection is effectively reduced.
4. According to the nutrition requirement characteristics of the young micropterus salmoides, the artificial compound feed meeting the nutrition requirement is used in a targeted manner, and the liver tissue lesions induced by liver fat and glycogen deposition of the young micropterus salmoides can be effectively relieved by combining scientific feeding quantity and proper feeding frequency, so that the liver function of the young micropterus salmoides is enhanced.
5. According to the nutrition requirements and metabolic characteristics of the micropterus salmoides, the growth performance of the micropterus salmoides is not affected under the condition of reducing the cost of the cultured feed and the labor cost by proper feeding limitation and feeding at proper feeding frequency, so that a certain feed cost and labor cost are saved, and the culture becomes more economical and feasible.
Drawings
Fig. 1 shows liver histological examination results of young micropterus salmoides under different treatments.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention.
Example 1:
the method provided by the invention is implemented and applied to a home dam experiment station of the Acipenser sinensis institute, and the specific implementation process is as follows:
according to the indoor culture characteristics, nutrition requirements and metabolism characteristics of young larch, the indoor culture liver function strengthening process is divided into three steps, namely: 1) Preparing a circulating water culture system; 2) Reducing stress response of juvenile fish; 3) Feeding and daily management.
Step 1) preparing two sets of circulating water culture systems comprising a water storage tank, a circulating tank and a culture tank, wherein one set of system is provided with a garlic water culture device (system 1) in the circulating tank, and the other set of system is not provided with a garlic water culture device (system 2). And (3) fully cleaning three pools and filtering facilities in the two sets of systems, putting tap water after cleaning, adding chlorine dioxide effervescent tablets for disinfection for 2 days. After the disinfection is finished, the disinfection water in the system is drained completely, tap water is added for flushing, the flushed tap water is drained completely, tap water is refilled, the circulating equipment and the oxygenation equipment are opened, and the water in the system is fully aerated for standby. And the germinated garlic cloves are placed in the holes of the garlic hydroponic device of the system 1.
And 2) after the juvenile fish are purchased back, placing the juvenile fish into a common rubber jar for temporary culture for 2 weeks. After the temporary rearing is finished, the weight of the juvenile fish is about 7g. After anaesthetizing juvenile fish by using an anaesthetic agent MS-222, disinfecting the juvenile fish for 1min by using aeration water with concentration of 3 per mill saline water in a circulating water culture system, and respectively placing the disinfected juvenile fish into a culture pond (6 ponds) of a system 1 and a culture pond (3 ponds) of a system 2 for culture, wherein the culture water body of each culture pond is 0.471m 3 The fish placement density is 30 tails/pool. Protection during fish layingThe difference between the temperature of the fish body and the water temperature in the culture system is less than 0.3 ℃ so as to reduce the stress of the young fish. After the juvenile fish is put into the pond, 14.1g of Vc stress agent is respectively put into 3 culture ponds in the system 1, and the inflow is further regulated to be small, so that the stress agent plays roles of reducing the stress of the juvenile fish and protecting the liver of the juvenile fish in the culture ponds. And the other culture ponds are not added with stress agent, and the water inflow is restored to be consistent after 2-3 hours.
Step 3) after the step 2 is completed, the young fish is fasted for 24 hours, and the health condition of the young fish is observed in the period. Feeding young fish with artificial compound feed with crude protein content of 53.7%, crude fat content of 14.2%, crude ash content of 13.8%, carbohydrate content of 9.8% and water content of 7.01% after fasted, wherein the feeding is carried out to apparent satiety in the first week, and starting from the second week, setting partial apparent satiety of young fish as a control, carrying out daily statistics on feeding conditions of apparent satiety, feeding other young fish according to 85% of the satiety amount, and feeding twice daily, wherein the feeding steps are respectively carried out at 9:00 and 17:00 feeding. The daily management flow is as follows:
and A, after feeding for 30min each time, fishing out the feed which is not ingested, so as to prevent the feed from polluting the culture water body. After 3h of feeding, the juvenile fish has discharged a large amount of excrement and urine, the juvenile fish excrement and urine is siphoned out of the system through the siphon, the pollution of the culture water body is reduced again, the actions of fishing residual baits and siphoning excrement and urine are slight, and the stress of the juvenile fish is reduced as much as possible.
3 weeks before the young fish is put into the water tank, the feed intake and the fecal excretion of the young fish are less, and the concentration of the pollutants in the culture water body rises slowly, so that the normal indexes of the pollutants in the culture water body can be ensured by changing 1/5 of the aerated tap water every day. Along with the growth of juvenile fish, the water exchange amount is required to be gradually increased, when the weight of the juvenile fish reaches more than 50g, water pollutants gather more quickly, the water exchange amount is increased to 1/3 at the moment so as to ensure that the content of the pollutants in the culture water is low, tail water of the culture pond is directly discharged out of the system through an increased pipeline when the water is exchanged, after the water is discharged to reach the volume required to be exchanged, the tail water drainage pipeline device is removed, and then fresh aerated standby tap water is injected into the circulating pond so as to achieve the purpose of water exchange.
And C, putting the fish for three weeks, wherein the weight of the young fish is small, the index change of the culture water body is slow, and the water quality detection is carried out once a week. After the juvenile fish grows to be more than 50g along with the cultivation process, the quality of the cultivation water body is detected twice a week. The detection ensures that the water temperature, the dissolved oxygen and the pH value of the culture water body are respectively maintained at 23.5+/-2.7 ℃, 8.72+/-0.34 mg/L and 7.58+/-0.14, and the ammonia nitrogen is ensured to be below 0.1 mg/L.
When the filtering system in the D circulating water culture system filters the water body, a large amount of pollutants are adsorbed on the filtering material, the weight of young fish is lower than 50g a few weeks before culture, the pollutant adsorption speed of the filtering material is not fast, and the filtering material needs to be thoroughly cleaned once a month. When the weight of the juvenile fish exceeds 50g, the cleaning frequency of the filter material is increased to be twice cleaning every month, so that the water quality index of the culture water body is ensured to be normal.
And (3) adding Vc stress agent into the stress agent group in the system 1 for 1 time every week in the E cultivation process. In addition, the health condition of all juvenile fish is observed every day, and whether the activity condition, swimming posture, feeding condition, defecation condition, fin and scale, body color change and the like are normal or not is mainly observed. The abnormal juvenile fish is fished out in time for anatomical observation, the body surface, fin, gill, liver and spleen are observed in focus, the death cause is searched as much as possible, parasites (ichthyophthirius, wheel worm and the like) and bacterial diseases (nocardia, flavobacterium columniformis and the like) are detected in an emphasized manner, and timely disease treatment measures are formulated.
Three kinds of cultivation treatments were set up in example one: the liver tissue section, the liver oxidation resistance state and the survival rate of the young micropterus salmoides under different treatment and cultivation are respectively detected and evaluated, the liver histological detection result is shown in figure 1, and the liver oxidation resistance state detection result and the survival rate calculation result are shown in table 1.
TABLE 1 liver antioxidant status and survival rate of young Japanese perch under different treatments
Note that: the data in the table are mean ± standard deviation, the different letters of the same row indicate significant differences (P < 0.05).
Conclusion of fig. 1 and table 1: compared with pure circulating water culture, the addition of the water culture garlic in the culture system can remarkably enhance the liver oxidation stress resistance of the young micropterus salmoides, improve the liver tissue morphology of the young micropterus salmoides and improve the survival rate of the young micropterus salmoides. When the water planting garlic and Vc stress agent are further combined in the circulating water culture system, the liver antioxidant stress capability of the young micropterus salmoides is further enhanced, the liver histology is further improved, and the survival rate is also further improved.
Example two
The method is applied to the aquatic college of southwest university, and the specific implementation process is as follows:
according to the indoor culture characteristics, nutrition requirements and metabolism characteristics of young larch, the indoor culture liver function strengthening process is divided into three steps, namely: 1) Preparing a circulating water culture system; 2) Reducing stress response of juvenile fish; 3) Feeding and daily management.
Step 1) preparing a circulating water culture system comprising a water storage tank, a circulating tank and a culture tank, wherein a garlic water culture device is arranged in the circulating tank of the system, and the total volume of the system is about 10m 3 . And (3) fully cleaning three tanks and filtering facilities in the system, putting tap water after cleaning, adding chlorine dioxide effervescent tablets for disinfection for 2 days. After the disinfection is finished, the disinfection water in the system is drained, tap water is added for flushing, the flushing tap water is drained, tap water is refilled, circulation equipment and oxygenation equipment are opened, the water in the system is fully aerated for standby, and meanwhile germinated garlic cloves are placed in holes of a garlic hydroponic device.
And 2) after the juvenile fish are purchased back, placing the juvenile fish into a common rubber jar for temporary culture for 2 weeks. After the temporary rearing is finished, the weight of the juvenile fish is about 7g. After anaesthetizing juvenile fish by using an anaesthetic agent MS-222, disinfecting the juvenile fish for 1min by using 3 per mill of salt water prepared by aeration water in a circulating water culture system, and placing the disinfected juvenile fish into the circulating water culture system for culture, wherein the fish placing density is 100 fish/m 3 . Keeping the difference between the temperature of the fish body and the water temperature in the culture system to be less than 0 in the fish placing processAnd 3 ℃ to reduce the stress of the juvenile fish. After the juvenile fish is put into the pond, 290g of Vc stress agent is put into the circulating pond, and the stress agent enters the culture pond in the systemic circulation process to play the roles of reducing the stress of the juvenile fish and protecting the liver of the juvenile fish.
Step 3) after the step 2 is completed, the young fish is fasted for 24 hours, and the health condition of the young fish is observed in the period. Feeding young fish with artificial compound feed with crude protein content of 53.7%, crude fat content of 14.2%, crude ash content of 13.8%, carbohydrate content of 9.8% and water content of 7.01% after fasted, wherein the feeding is carried out to apparent satiety in the first week, and starting from the second week, setting partial apparent satiety of young fish as a control, carrying out daily statistics on feeding conditions of apparent satiety, feeding other young fish according to 85% of the satiety amount, and feeding twice daily, wherein the feeding steps are respectively carried out at 9:00 and 17:00 feeding. The daily management flow is as follows:
A. after each feeding for 30min, the feed which is not ingested is fished out, so that the pollution of the culture water body is prevented. After 3h of feeding, the juvenile fish has discharged a large amount of excrement and urine, the juvenile fish excrement and urine is siphoned out of the system through the siphon, the pollution of the culture water body is reduced again, the actions of fishing residual baits and siphoning excrement and urine are slight, and the stress of the juvenile fish is reduced as much as possible.
B. 3 weeks before the young fish is put into the water tank, the feed intake and the fecal excretion of the young fish are less, and the concentration of the pollutants in the culture water body rises slowly, so that the normal indexes of the pollutants in the culture water body can be ensured by changing 1/5 of the aerated tap water every day. Along with the growth of juvenile fish, the water exchange amount is required to be gradually increased, when the weight of the juvenile fish reaches more than 50g, water pollutants gather more quickly, the water exchange amount is increased to 1/3 at the moment so as to ensure that the content of the pollutants in the culture water is low, tail water of the culture pond is directly discharged out of the system through an increased pipeline when the water is exchanged, after the water is discharged to reach the volume required to be exchanged, the tail water drainage pipeline device is removed, and then fresh aerated standby tap water is injected into the circulating pond so as to achieve the purpose of water exchange.
C. The weight of the young fish is small three weeks before the fish is put, the index change of the culture water body is slow, and the water quality detection is carried out once a week. After the juvenile fish grows to be more than 50g along with the cultivation process, the quality of the cultivation water body is detected twice a week. The detection ensures that the water temperature, the dissolved oxygen and the pH value of the culture water body are respectively maintained at 25.6+/-1.8 ℃ and 8.52+/-0.44 mg/L and 7.60+/-0.06, and ensures that the ammonia nitrogen is below 0.1 mg/L.
D. When the filtering system in the circulating water culture system filters the water body, a large amount of pollutants are adsorbed on the filtering material, the weight of young fishes is lower than 50g a few weeks before culture, the pollutant adsorption speed of the filtering material is not fast, and the filtering material needs to be thoroughly cleaned once a month. When the weight of the juvenile fish exceeds 50g, the cleaning frequency of the filter material is increased to be twice cleaning every month, so that the water quality index of the culture water body is ensured to be normal.
E. In the whole cultivation process, stress agent is added into the system once every week, the health condition of young fish is observed every day, and whether the activity condition, swimming posture, ingestion condition, defecation condition, fin and scale, body color change and the like are normal or not is mainly observed. The abnormal juvenile fish is fished out in time for anatomical observation, the body surface, fin, gill, liver and spleen are observed in focus, the death cause is searched as much as possible, parasites (ichthyophthirius, wheel worm and the like) and bacterial diseases (nocardia, flavobacterium columniformis and the like) are detected in an emphasized manner, and timely disease treatment measures are formulated.
The survival rate of the young perch in the second example reaches 95.2 percent.
The foregoing embodiments are merely illustrative of the technical idea and features of the present invention, and the present invention is not limited to the preferred embodiments. Within the technical scope of the present disclosure, the technical solution and the invention concept according to the present disclosure are equivalent to or improved from the above description, and all the equivalent changes or modifications are included in the scope of the present disclosure.
Claims (10)
1. An indoor culture method for young largemouth bass is characterized by comprising the following steps:
s1, preparing an indoor circulating water culture system, storing water after cleaning and sterilizing, circulating, aerating and simultaneously carrying out water culture on garlic;
s2, disinfecting young largemouth bass by adopting saline solution, and then placing the young largemouth bass into a circulating water culture system in the S1, wherein the difference between the body temperature of the young largemouth bass and the water temperature in the system is less than 0.5 ℃ in the process of putting the young largemouth bass into the pond; the density of the young fish is at8kg/m 3 The young fish is put into the pool and then anti-stress animal protection products are sprayed in the system;
s3, feeding feed after the young fish is put into the pond and fasted for 20-24 hours, cleaning the culture system in time, monitoring the water quality, controlling the water temperature of the water body at 22-27 ℃, and maintaining the dissolved oxygen at 7-8 and the ammonia nitrogen below 0.1 mg/L.
2. The method for indoor cultivation of young largemouth bass of claim 1, wherein: the indoor circulating water culture system comprises a water storage aeration tank, a circulating tank, a culture tank, a water pump and corresponding water injection and outlet pipes, wherein a step filtering facility is arranged in the circulating tank, the water outlet pipe of the circulating tank is connected to a water injection port of the culture tank, the water injection port of the culture tank is provided with the filtering facility, and the water outlet of the culture tank is connected to the water injection port of the circulating tank through a pipe; the water outlet of the water storage aeration tank is connected to the water filling port of the circulating tank through a pipeline.
3. The method for indoor cultivation of young largemouth bass according to claim 2, wherein: the method comprises the steps of placing germinated garlic cloves on a water culture foam plate for fixation during water culture, floating the garlic cloves in a water body of the circulating pool for cultivation, wherein the density of the garlic cloves is 180-220 grains/m 2 And (5) controlling.
4. The method for indoor cultivation of young largemouth bass of claim 1, wherein: the mass concentration of the saline water in the S2 is 3-5 mill, and the saline water is prepared by adopting aerated water in a circulating water culture system.
5. The method for indoor cultivation of young largemouth bass according to claim 2, wherein: the anti-stress animal protection product in S2 is Vc stress agent with the concentration of 28-32 g/m 3 The method comprises the steps of carrying out a first treatment on the surface of the It is configured in a circulation tank and the subsequent application frequency is kept 1 time per week.
6. The method for indoor cultivation of young largemouth bass of claim 1, wherein: in the feed for juvenile fish in S3, the content of carbohydrate is below 10 percent, the content of fat is below 15 percent and the content of protein is above 45 percent.
7. The method for indoor cultivation of young largemouth bass of claim 1, wherein: and S3, when the feed is fed, the feeding level is 80-85% of the apparent satiety feeding amount, and the feeding frequency is 2-3 times/day.
8. The method for indoor cultivation of young largemouth bass of claim 1, wherein: in the S, food residues and excrement are timely discharged in the cultivation process; the feed which is not ingested in half an hour after feeding and the defecation of the fed juvenile fish after ingestion are discharged in time.
9. The method for indoor cultivation of young largemouth bass according to claim 2, wherein: and S3, changing water at least 1/5 of the water in the culture system every day, discharging the changed water out of the culture system, and supplementing water stored in a reservoir in advance and aerated for more than one day into a circulating pool of the circulating water culture system.
10. Use of the method of any one of claims 1-9 for enhancing liver function in young fish.
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