CN116875586A - Preparation method and application of oleophylic and hydrophobic high-activity microorganism-loaded composite cellulose/luffa aerogel - Google Patents
Preparation method and application of oleophylic and hydrophobic high-activity microorganism-loaded composite cellulose/luffa aerogel Download PDFInfo
- Publication number
- CN116875586A CN116875586A CN202310756731.8A CN202310756731A CN116875586A CN 116875586 A CN116875586 A CN 116875586A CN 202310756731 A CN202310756731 A CN 202310756731A CN 116875586 A CN116875586 A CN 116875586A
- Authority
- CN
- China
- Prior art keywords
- hec
- aerogel
- microorganism
- luffa
- cellulose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000004964 aerogel Substances 0.000 title claims abstract description 108
- 244000005700 microbiome Species 0.000 title claims abstract description 84
- 229920002678 cellulose Polymers 0.000 title claims abstract description 40
- 239000001913 cellulose Substances 0.000 title claims abstract description 40
- 230000000694 effects Effects 0.000 title claims abstract description 38
- 230000002209 hydrophobic effect Effects 0.000 title claims abstract description 36
- 239000002131 composite material Substances 0.000 title claims abstract description 35
- 235000003956 Luffa Nutrition 0.000 title claims abstract description 33
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 244000050983 Luffa operculata Species 0.000 title 1
- 241000219138 Luffa Species 0.000 claims abstract description 32
- 239000000463 material Substances 0.000 claims abstract description 32
- 230000015556 catabolic process Effects 0.000 claims abstract description 23
- 238000006731 degradation reaction Methods 0.000 claims abstract description 23
- 230000000593 degrading effect Effects 0.000 claims abstract description 12
- 238000001179 sorption measurement Methods 0.000 claims abstract description 12
- 239000000725 suspension Substances 0.000 claims abstract description 9
- 230000001580 bacterial effect Effects 0.000 claims abstract description 6
- 238000012258 culturing Methods 0.000 claims abstract description 3
- 239000000243 solution Substances 0.000 claims description 32
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 30
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 claims description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- 238000000034 method Methods 0.000 claims description 17
- 239000008367 deionised water Substances 0.000 claims description 15
- 229910021641 deionized water Inorganic materials 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 15
- UWFRVQVNYNPBEF-UHFFFAOYSA-N 1-(2,4-dimethylphenyl)propan-1-one Chemical compound CCC(=O)C1=CC=C(C)C=C1C UWFRVQVNYNPBEF-UHFFFAOYSA-N 0.000 claims description 13
- 239000002033 PVDF binder Substances 0.000 claims description 12
- 229920002981 polyvinylidene fluoride Polymers 0.000 claims description 12
- 241000894006 Bacteria Species 0.000 claims description 11
- 238000002156 mixing Methods 0.000 claims description 11
- 239000002283 diesel fuel Substances 0.000 claims description 10
- 238000011068 loading method Methods 0.000 claims description 10
- 239000003208 petroleum Substances 0.000 claims description 9
- 239000011148 porous material Substances 0.000 claims description 8
- 241000589516 Pseudomonas Species 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 7
- 239000011259 mixed solution Substances 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 6
- 238000004108 freeze drying Methods 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 239000003431 cross linking reagent Substances 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 239000000835 fiber Substances 0.000 claims description 4
- 244000280244 Luffa acutangula Species 0.000 claims description 3
- 235000009814 Luffa aegyptiaca Nutrition 0.000 claims description 3
- 239000000969 carrier Substances 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 239000011248 coating agent Substances 0.000 claims description 3
- 238000000576 coating method Methods 0.000 claims description 3
- 238000004132 cross linking Methods 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 229930195733 hydrocarbon Natural products 0.000 claims description 3
- 150000002430 hydrocarbons Chemical class 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 239000003209 petroleum derivative Substances 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 238000007873 sieving Methods 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- 241000589291 Acinetobacter Species 0.000 claims description 2
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 2
- 241000187654 Nocardia Species 0.000 claims description 2
- 241000228143 Penicillium Species 0.000 claims description 2
- 239000003153 chemical reaction reagent Substances 0.000 claims description 2
- 238000004090 dissolution Methods 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 239000000758 substrate Substances 0.000 claims description 2
- 238000009631 Broth culture Methods 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 230000007935 neutral effect Effects 0.000 claims 1
- 229920006395 saturated elastomer Polymers 0.000 claims 1
- 238000012986 modification Methods 0.000 abstract description 13
- 230000004048 modification Effects 0.000 abstract description 13
- 238000011160 research Methods 0.000 abstract description 7
- 239000000126 substance Substances 0.000 abstract description 7
- 230000009286 beneficial effect Effects 0.000 abstract description 5
- 150000001335 aliphatic alkanes Chemical class 0.000 abstract description 4
- 239000003795 chemical substances by application Substances 0.000 abstract description 3
- 239000012141 concentrate Substances 0.000 abstract description 2
- 230000002708 enhancing effect Effects 0.000 abstract description 2
- 238000001878 scanning electron micrograph Methods 0.000 description 9
- 239000003344 environmental pollutant Substances 0.000 description 6
- 231100000719 pollutant Toxicity 0.000 description 6
- 238000007667 floating Methods 0.000 description 4
- 238000000026 X-ray photoelectron spectrum Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 238000006065 biodegradation reaction Methods 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 239000002068 microbial inoculum Substances 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 241000219122 Cucurbita Species 0.000 description 2
- 235000009852 Cucurbita pepo Nutrition 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000012876 carrier material Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- DCAYPVUWAIABOU-UHFFFAOYSA-N hexadecane Chemical compound CCCCCCCCCCCCCCCC DCAYPVUWAIABOU-UHFFFAOYSA-N 0.000 description 2
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- NPGIHFRTRXVWOY-UHFFFAOYSA-N Oil red O Chemical compound Cc1ccc(C)c(c1)N=Nc1cc(C)c(cc1C)N=Nc1c(O)ccc2ccccc12 NPGIHFRTRXVWOY-UHFFFAOYSA-N 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000011358 absorbing material Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 230000003749 cleanliness Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- VUZPPFZMUPKLLV-UHFFFAOYSA-N methane;hydrate Chemical compound C.O VUZPPFZMUPKLLV-UHFFFAOYSA-N 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000003305 oil spill Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000007420 reactivation Effects 0.000 description 1
- 235000011149 sulphuric acid Nutrition 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
- C12N11/12—Cellulose or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/10—Nature of the water, waste water, sewage or sludge to be treated from quarries or from mining activities
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/38—Pseudomonas
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/80—Penicillium
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Mycology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
The invention belongs to the technical field of chemical application, and particularly relates to a preparation method and application of oleophylic and hydrophobic high-activity microorganism-loaded composite cellulose/luffa aerogel, which comprises the following steps: culturing bacterial suspension, extracting luffa cellulose (LS), preparing HEC/LS composite aerogel material, preparing loaded microorganism Bn-HEC/LS aerogel, and preparing oil-hydrophobic high-activity loaded microorganism Bn-HEC/LS aerogel. The invention adopts the adhesion agent to concentrate the loaded microorganism for many times and selectively adsorb the hydrophobic modification to play a key role in improving the degradation rate of the material, and the adsorption property of the material is beneficial to enhancing the contact rate of the material with diesel, and through the research of application of the material, the oleophylic and hydrophobic high-activity loaded microorganism composite cellulose/luffa aerogel prepared in the invention is primarily considered to have better activity of degrading normal alkane, and has stronger tolerance to surrounding inferior environments, thus having certain practical application value.
Description
Technical Field
The invention belongs to the technical field of chemical application, and particularly relates to a preparation method and application of oleophylic and hydrophobic high-activity microorganism-loaded composite cellulose/luffa aerogel.
Background
Petroleum pollution is derived from exploration, production and transportation activities of the petroleum industry. Considering the wide pollution and problems of oily wastewater, it is urgent to develop an efficient and economical oily wastewater treatment method. Various restoration techniques of the existing oily wastewater mainly comprise heat treatment, physical, chemical and biological treatment methods. Traditional heat treatment, physical, chemical methods have proven effective, but in most cases they are not economically viable and often produce secondary pollution. Among them, bioremediation by microorganisms has been widely used in recent years for purifying petroleum pollutants because of its advantages of high efficiency, cleanliness, economy, no secondary waste, and the like. However, the degradation efficiency using free microorganisms is greatly limited because microorganisms are difficult to retain in the environment for a long period of time, have low activity, and are sensitive to environmental factors. And immobilized microorganism technology can solve the problems well. Compared with free microorganisms, the immobilized microorganisms have the advantages of higher microorganism activity, stronger tolerance to harmful environmental conditions, prevention of microorganism loss and the like, and can improve the degradation efficiency of petroleum pollutants. The cellulose/luffa aerogel has the advantages of good compatibility as an immobilized microorganism material, environmental protection, good microenvironment for biology, low price and the like. In recent years, immobilized microorganism technology has become a hotspot in petroleum pollutant biodegradation research. However, most of the researches have focused on external factors that may limit the biodegradation process, such as pH, temperature, carrier materials, etc., while few researches on selective adsorption and biodegradation of petroleum pollutants have been conducted. Therefore, it is of great importance to further seek new carrier materials with high efficiency and strong self-floating performance. Therefore, the construction of the supported microorganism composite aerogel with stable structure, strong self-floating performance and high activity and high performance is extremely important for the research on the performance of degrading normal alkane.
Disclosure of Invention
The invention aims at solving the problems existing in the prior art and provides oleophylic and hydrophobic high-activity microorganism-loaded cellulose/luffa aerogel as well as a preparation method and application thereof.
In order to achieve the above object, the present invention provides the following technical solutions:
the preparation method of the oleophylic and hydrophobic high-activity microorganism-loaded composite cellulose/luffa aerogel is characterized by comprising the following steps of:
step one, culturing bacterial suspension
Bacillus (BTS), pseudomonas (PA), acinetobacter (AL), nocardia (NL) and Penicillium (PO) are selected bacteria with high efficiency degradation to petroleum hydrocarbons; about 100. Mu.L of the active strain is inoculated into a sterile nutrient broth medium and subjected to shaking culture at 37 ℃ and 120r min-1; after 48 hours, observing turbidity, and the colony count reaches more than 1X 108cfu mL < -1 >; the mixed microorganism liquid is prepared according to the same volume ratio, taken out and stored in a refrigerator at 4 ℃.
Step two, extraction of luffa cellulose (LS)
First of all,crushing 20g of dried retinervus Luffae fructus (designated SPLS), and sieving with 80 mesh sieve to obtain pretreated retinervus Luffae fructus fiber (designated PLS); next, PLS was hydrolyzed with NaOH (PLS: naOH bath ratio 1:20) at 98℃with continuous stirring for 4h; then, at H 2 SO 4 (PLS:H 2 SO 4 Soaking in the bath ratio of 1:20) for 10min, filtering and washing to neutrality; finally, the luffa cellulose is obtained by constant temperature drying at 50 ℃, and is named LS;
step three, preparation of HEC/LS composite aerogel material
Firstly, 10g of luffa cellulose (named LS) is dissolved in NaOH solution and then added into 60g of deionized water for dissolution; then, adding 4g of cellulose (named HEC) powder into 96g of deionized water, standing for 0.5h, and repeatedly stirring and standing until the cellulose is completely dissolved in the deionized water to obtain a 4% HEC solution; secondly, mixing the HEC solution with the mass fraction of 4% and the LS solution with the mass fraction of 10% according to the following weight ratio of 1:1 blending, adding a cross-linking agent ethylene glycol diglycidyl ether (EDGE), wherein the EDGE dosage is 50% of the dry weight of HEC and LS in the mixture, and mixing for 0.5h at 80 ℃ to obtain an HEC/LS blend; finally, freeze-drying the HEC/LS mixture for 48 hours in a freeze dryer, repeatedly washing with deionized water to remove sodium hydroxide in the composite material, then placing the composite material into a refrigerator at the temperature of minus 20 ℃ for 24 hours, and finally obtaining the composite cellulose/luffa aerogel after freeze drying, which is named HEC/LS;
step four, preparing microorganism Bn-HEC/LS aerogel
Sterilizing the HEC/LS aerogel obtained in the step three in an autoclave at 121 ℃ for 30 minutes for later use;
HEC/LS aerogel immobilized microorganisms are divided into three steps: firstly, enabling HEC/LS sponge to completely adsorb 3% PVA solution, removing redundant PVA through filtration, and preventing the internal pores of aerogel from being blocked; secondly, immersing HEC/LS sponge into mixed microorganism suspension (BTS: NL: PO: PA: AL mixed ratio is 1:1:1) until adsorption reaches saturation, and drying at 30 ℃ for 1 hour; repeating the steps for a plurality of times until the adsorption reaches saturation; finally, ca (NO) 3 ) 2 The solution is subjected to a crosslinking reaction at 0-4deg.C for 30min, and dried at 30deg.CDrying for 1h, and suction filtering to remove excessive Ca (NO 3 ) 2 A solution; compared with the traditional method, the multi-concentration method enhances the adhesiveness between microorganisms and materials, reduces the risk of blocking holes by PVA, increases the quantity of immobilized microorganisms, and enables a large number of microorganisms to be firmly loaded in HEC/LS aerogel; repeating the operation for 5 times, wherein the carriers after the 1 st, 2 nd, 3 rd, 4 th and 5 th times of microorganism loading are respectively named as B1-HEC/LS, B2-HEC/LS, B3-HEC/LS, B4-HEC/LS and B5-HEC/LS;
step five, preparation of oleophylic and hydrophobic high-activity loaded microorganism Bn-HEC/LS aerogel
Taking the material (B5-HEC/LS) loaded with the petroleum hydrocarbon degrading bacteria for 5 times in the fourth step as a subsequent oleophylic and hydrophobic modified material; firstly, adding polyvinylidene fluoride (PVDF) into N, N Dimethylformamide (DMF) solution, and stirring for 1h at normal temperature to fully mix the polyvinylidene fluoride (PVDF); then, the B5-HEC/LS sponge is quickly immersed into the mixed solution; finally, the material immersed in the mixed solution is quickly taken out and put into distilled water to form a hydrophobic coating on the surface. The resulting hydrophobically oleophilic modified material was designated OB5-HEC/LS aerogel.
Preferably, in the third step, the step of repeating the operation is 10 times.
Preferably, in the third step, the LS solution is a viscous solution with LS content of 10%, and the mass of the cross-linking agent ethylene glycol diglycidyl ether (EDGE) is 7g.
Preferably, in the fourth step, the step of repeating the operation is 4 times.
Preferably, in the fourth step, quantitative bacterial suspension is added into the HEC/LS, and the 1 st, 2 nd, 3 rd, 4 th and 5 th times of microorganism loading amounts are respectively as follows: 5.5853g, 8.2214g, 10.8963g, 12.46996g, 13.3521g.
Preferably, in step four, the Ca (NO 3 ) 2 The concentration of (2) was 5% and the amount added was 20mL.
Preferably, in the fifth step, the ratio of PVDF to DMF in the hydrophobically modified reagent is 1:10.
the invention also provides oleophylic and hydrophobic high-activity microorganism-loaded composite cellulose/loofah sponge aerogel, which is prepared by the preparation method.
The invention also provides application of the oleophylic and hydrophobic high-activity microorganism-loaded composite cellulose/luffa aerogel in diesel oil degradation.
Preferably, the substrate is a mixture of 1% diesel and 30mL deionized water.
The invention has the following beneficial effects:
1. the invention introduces a method for synthesizing the oleophylic and hydrophobic high-activity microorganism-loaded composite cellulose/luffa aerogel by taking hydrophilic ultralight modification, repeated concentration of microorganism-loaded and oleophylic and hydrophobic selective modification as a main means.
2. The invention researches the petroleum degradation performance in hydrocarbon diesel.
3. The invention adopts the adhesion agent to concentrate the loaded microorganism for many times and selectively adsorb the hydrophobic modification to play a key role in improving the degradation rate of the material, and the adsorption property of the material is beneficial to enhancing the contact rate of the material with diesel, and through the research of application of the material, the oleophylic and hydrophobic high-activity loaded microorganism composite cellulose/luffa aerogel prepared in the invention is primarily considered to have better activity of degrading normal alkane, and has stronger tolerance to surrounding inferior environments, thus having certain practical application value.
Drawings
FIG. 1 is a route diagram of the preparation of the oleophilic, hydrophobic, high activity loaded microorganism composite cellulose/retinervus Luffae aerogel of the present invention;
in fig. 2, fig. 2 (a) is an SEM image of the inside of the retinervus Luffae fructus; FIG. 2 (b) is an SEM image of the HEC aerogel surface; FIG. 2 (c) is an SEM image of the HEC/LS aerogel surface; FIG. 2 (d) is an SEM image of the interior of HEC/LS aerogel;
in FIG. 3, FIG. 3 (a) is a mass change curve of a mixed microorganism of HEC/LS aerogel fixed 1-8 times; FIG. 3 (b-d) are SEM photographs of 1, 3 and 5 times the immobilization of HEC/LS aerogel.
In FIG. 4, FIG. 4 (a) shows XPS spectra of (a) before and after modification of HEC/LS aerogel; FIG. 4 (B) is the self-floatability of HEC aerogel, HEC/LS aerogel before modification (B) and after modification (C); FIG. 4 (c) is a graph comparing the adsorptivity of HEC/LS aerogel before and after modification to oil;
in FIG. 5, FIG. 5 (a) is a gas chromatograph (GC-MS) original image of OB5-HEC/LS aerogel degraded diesel; FIG. 5 (B) shows the degradation rates of microorganisms, HEC/LS aerogel, B5-HEC/LS aerogel and OB5-HEC/LS aerogel at 37℃at 120 r/min, pH=7, and diesel concentration of 1%.
Detailed Description
The present invention will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
The preparation route of the oleophylic and hydrophobic high-activity microorganism-loaded composite cellulose/luffa aerogel is shown in figure 1.
Example one, preparation of luffa cellulose (LS) by extraction
Pulverizing retinervus Luffae fructus, and sieving with 80 mesh sieve to obtain pretreated retinervus Luffae fructus fiber (PLS). PLS was hydrolyzed with 15% NaOH (PLS: naOH bath ratio 1:20) at 98 ℃ for 4h with continuous stirring; then soaking 10min in 1.0g/l H SO4 (PLS: H2SO4 bath ratio 1:20), filtering and washing to neutrality; finally, luffa cellulose (LS) was obtained by constant temperature drying at 50 ℃.
Example two preparation of composite HEC/LS aerogel Material
10g of retinervus Luffae fructus (designated LS) was dissolved in 30g of 5wt% NaOH solution and then dissolved in 60g of deionized water. 4g of cellulose (named HEC) powder is added into 96g of deionized water, and the mixture is kept stand for 0.5h, and then repeatedly stirred and kept stand until the cellulose powder is completely dissolved in the deionized water, so that a 4% HEC solution is obtained. Mixing the HEC solution with the mass fraction of 4% and the LS solution with the mass fraction of 10% according to the following weight ratio of 1:1, adding a cross-linking agent ethylene glycol diglycidyl ether (EDGE), wherein the EDGE dosage is 50% of the dry weight of HEC and LS in the mixture, and mixing for 0.5h at 80 ℃ to obtain the HEC/LS blend. And then, freeze-drying the HEC/LS mixture in a freeze dryer for 48 hours, washing with deionized water for multiple times to remove sodium hydroxide in the composite material, then placing the composite material in a refrigerator at the temperature of minus 20 ℃ for 24 hours, and finally obtaining the composite cellulose/luffa aerogel after freeze drying, which is named HEC/LS.
As shown in fig. 2, wherein fig. 2 (a) is an SEM image of the inside of the retinervus Luffae fructus; FIG. 2 (b) is an SEM image of the HEC aerogel surface; FIG. 2 (c) is an SEM image of the HEC/LS aerogel surface; FIG. 2 (d) SEM image of the interior of HEC/LS aerogel.
As shown in a Scanning Electron Microscope (SEM) image of the inside of the towel gourd, the surface and pores of the inside of the natural towel gourd fiber are very smooth, and the pores are too large, so that the immobilization of microorganisms is not facilitated, and the microorganisms are easy to leak. The HEC aerogel of fig. 2 (b) has a very smooth surface and no pores, which is detrimental to the immobilization of the microorganisms. As shown in FIG. 2 (c-d), HEC/LS aerogel has holes on the surface and coarse vertically arranged channel structure inside, and has communication holes, which are more beneficial to transfer and transportation of pollutants after being fully contacted with microorganisms in the carrier. Therefore, HEC/LS aerogel is more suitable for immobilization of microorganisms.
Example III preparation of immobilized microorganism porous Material
HEC/LS aerogel Synthesis: the same as in the second embodiment.
HEC/LS aerogel was sterilized in an autoclave at 121℃for 30 minutes. HEC/LS aerogel immobilized microorganisms are divided into three steps. First, the HEC/LS sponge fully adsorbed the 3% PVA solution, and excess PVA was removed by filtration, preventing the internal pores of the aerogel from plugging. Next, the HEC/LS sponge was immersed in the mixed microbial suspension (BTS: NL: PO: PA: AL mixing ratio 1:1:1) until adsorption reached saturation, and dried at 30℃for 1 hour. The second step is repeated a number of times until the adsorption reaches saturation. Finally, ca (NO) 3 ) 2 The solution was subjected to a crosslinking reaction at 0-4℃for 30min and dried at 30℃for 1h. Drying at 30℃for 1 hour. Filtering out excessive Ca (NO) 3 ) 2 A solution. Compared with the traditional method, the multi-concentration method enhances the adhesiveness between microorganisms and materials, reduces the risk of blocking holes by PVA, increases the number of immobilized microorganisms, and enables a large number of microorganisms to be firmly loaded in HEC/LS aerogel. The operation was repeated 5 times. 1. 2, 3, 4,The carriers after 5 times of microorganism loading are respectively named as B1-HEC/LS, B2-HEC/LS, B3-HEC/LS, B4-HEC/LS and B5-HEC/LS.
As shown in FIG. 3, the effect of the amount of immobilized bacteria on HEC/LS aerogel morphology was further investigated. Wherein FIG. 3 (a) is a mass change curve of HEC/LS aerogel immobilized 1-8 times mixed microorganisms; FIG. 3 (b-d) are SEM photographs of 1, 3 and 5 times the immobilization of HEC/LS aerogel.
As shown in fig. 3 (a), the experiment showed that the number of immobilized microorganisms tended to saturate with increasing concentration. Furthermore, calculations indicate that the number of viable bacteria increases slowly with increasing loading time. Calculation of the immobilized microorganism number shows that as the microorganism loading times increase, the viable bacteria number also increases, wherein 5 times are the optimal microorganism immobilization times, so after comprehensive analysis, the patent selects 5 times as the optimal loading times. SEM images of the mixed bacteria immobilized 1, 3, 5 times in HEC/LS aerogel are shown in FIG. 3 (b-d). Along with the increase of the immobilization times, the distribution of microorganisms is more and more dense, which indicates that the multi-time immobilization concentrated microbial inoculum greatly improves the survival rate of microorganisms and provides possibility for efficiently degrading pollutants.
Example IV preparation of oleophilic hydrophobic high Activity loaded microorganism Bn-HEC/LS aerogel
Preparation of Bn-HEC/LS aerogel: as in the third embodiment.
Polyvinylidene fluoride (PVDF) was added to the N, N Dimethylformamide (DMF) solution and stirred at room temperature for 1h to allow thorough mixing. Then, the B5-HEC/LS sponge was quickly immersed in the above mixed solution. Finally, the material immersed in the mixed solution is quickly taken out and put into distilled water to form a hydrophobic coating on the surface. The resulting hydrophobically oleophilic modified material was designated OB5-HEC/LS aerogel.
As shown in fig. 4, wherein fig. 4 (a) is an XPS spectrum of (a) before and after HEC/LS aerogel modification; FIG. 4 (B) is the self-floatability of HEC aerogel, HEC/LS aerogel before modification (B) and after modification (C); FIG. 4 (c) is a graph comparing the adsorptivity of HEC/LS aerogel before and after modification to oil.
As shown in FIG. 4 (a), the HEC/LS aerogel mainly contains elements C1 s and O1s, and the modified HEC/LS aerogel shows the element F1 s in XPS spectrum, which indicates that the surface of the HEC/LS aerogel is successfully modified by hydrophobic and oleophilic agents. As shown in FIG. 4 (b), the HEC aerogel has settled after absorbing water, and the HEC/LS aerogel and the modified HEC/LS aerogel still float on the water surface and have excellent self-floating ability, and can keep stable long-term floating ability for more than 30 days. Therefore, HEC/LS aerogel has great potential in treating water surface pollution such as oil spill on the sea. As shown in FIG. 4 (c), the performance of HEC/LS aerogel in oil purification is reflected. Diesel is dyed with oil red O to facilitate observation, and within 1mi n, the modified HEC/LS will oil layer with no diesel present around the fully absorbing material, while the unmodified HEC/LS aerogel will have little diesel present around the absorbing diesel material.
Fifth embodiment, the application of the oleophylic and hydrophobic high-activity loaded microorganism HEC/LS aerogel in degrading normal alkane
30mL of deionized water and 1% (relative to the fixed amount of microbial inoculum) are added into a 50mL conical flask, and the OB5-HEC/LS aerogel after the reactivation of the previous preparation is put into the conical flask, and is degraded for 10 hours, 20 hours, 30 hours, 40 hours, 50 hours, 60 hours, 70 hours and 80 hours under the conditions of 37 ℃, 120 r/min, pH=7 and the like. And extracting the degraded mixed solution and the material with n-hexane, repeatedly extracting for three times in a volumetric flask with the volume of 25mL/50mL, and testing the absorbance of diesel oil by using an ultraviolet spectrophotometer (MAPADA P2 PC) after the volume is fixed. The degradation rate was calculated by a standard curve by quantitatively analyzing it by GC-MC.
As shown in fig. 5, wherein fig. 5 (a) is a gas chromatograph (GC-MS) original diagram of OB5-HEC/LS aerogel degraded diesel;
FIG. 5 (B) shows the degradation rates of microorganisms, HEC/LS aerogel, B5-HEC/LS aerogel and OB5-HEC/LS aerogel at 37℃at 120 r/min, pH=7, and diesel concentration of 1%.
As shown in fig. 5 (a), the peak area of the sample gradually decreased with increasing degradation time, indicating that the diesel residue gradually decreased and degraded with the passage of time. The carrier forms a micro-reactor, which can provide carbon source and the like, so that the petroleum hydrocarbon degrading bacteria can keep activity in the reactor, can grow, differentiate and reproduce, protect the degrading bacteria, and prevent the loss of cell products such as biological degrading enzyme and the like, thereby degrading the diesel oil into micromolecular inorganic substances, carbon dioxide and water.
As shown in fig. 5 (B), under the conditions of initial concentration 3%, ph=7 and 37 ℃, comparison of B5-HEC/LS aerogel (experimental group 1), OB5-HEC/LS aerogel (experimental group 2), free mixed bacteria (control group) and HEC/LS aerogel (blank group) shows that the degradation rate gradually becomes higher with increasing degradation time, after degradation for 160 hours, the degradation rates of B5-HEC/LS aerogel and OB5-HEC/LS aerogel all show a tendency to be stable, at 180 hours, the degradation rate of OB5-HEC/LS aerogel reaches 94.75% to the maximum, and B5-HEC/LS aerogel reaches 71.39%, and free mixed microorganisms of the control group tend to be stable after 24 hours. Analysis of the above results may be because, when the free mixed microorganism just enters the water-oil mixture containing 1% of diesel oil, the diesel oil is rapidly absorbed and degraded to obtain energy due to the nutrient substances required for bacterial growth and reproduction, or the diesel oil is converted into its constituent substances through assimilation, and the microorganism dies largely due to insufficient carbon source in the later stage of degradation, and the degradation rate tends to be low and continues. Analysis of the blank group degradation curve shows that the material has an adsorption effect, can effectively improve the biological effective concentration and activity in the microenvironment of the B5-HEC/LS aerogel and the OB5-HEC/LS aerogel, enhances the selective adsorption of the material to the diesel oil, is beneficial to improving the contact rate of the material with the diesel oil, and further enables the material to generate extremely high degradation rate to the diesel oil. Meanwhile, the method for researching the microorganism loading makes up the defect of weak binding force of the traditional adsorption method, adopts PVA to firmly load the microorganism loading on the pores of the material, and then passes through Ca 2+ The PVA is crosslinked to fix the shape and firmly adhere to the microbial inoculum, so that the degradation efficiency of the hexadecane is obviously improved. The OB5-HEC/LS aerogel is selectively adsorbed and modified on the basis of the B5-HEC/LS aerogel, so that the surface tension is reduced, the hydrophobic and oleophylic effects are shown, and the degradation rate of the B5-HEC/LS aerogel is improved by 23.36%.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the invention.
Claims (10)
1. The preparation method of the oleophylic and hydrophobic high-activity microorganism-loaded composite cellulose/luffa aerogel is characterized by comprising the following steps of:
step one, culturing bacterial suspension
Bacillus (BTS), pseudomonas (PA), acinetobacter (AL), nocardia (NL) and Penicillium (PO) are selected bacteria with high efficiency degradation to petroleum hydrocarbons; about 100. Mu.L of the active strain is inoculated into a sterile nutrient broth culture medium and subjected to shaking culture at 37 ℃ under the condition of 120 rmin-1; after 48 hours, observing turbidity, and the colony count reaches more than 1 multiplied by 108cfumL < -1 >; preparing mixed microorganism liquid according to the same volume ratio, taking out and storing in a refrigerator at 4 ℃;
step two, extraction of luffa cellulose (LS)
Firstly, crushing 20g of dried loofah sponge (named SPLS), and sieving by using an 80-mesh sieve to obtain pretreated loofah fiber (named PLS); next, PLS was continuously stirred with 30ml of 5wt% NaOH at 98℃for 4h with a PLS: naOH bath ratio of 1:20; then, at H 2 SO 4 Soaking in the solution for 10min, PLS:H 2 SO 4 The bath ratio of (2) is 1:20, and the solution is filtered and washed to be neutral; finally, the luffa cellulose is obtained by constant temperature drying at 50 ℃, and is named LS;
step three, preparation of HEC/LS composite aerogel material
Firstly, 10g of luffa cellulose (LS) is dissolved in 30g of 5wt% NaOH solution and then added into 60g of deionized water for dissolution; then, adding 4g of cellulose (named HEC) powder into 96g of deionized water, standing for 0.5h, and repeatedly stirring and standing until the cellulose is completely dissolved in the deionized water to obtain a 4% HEC solution; secondly, mixing the HEC solution with the mass fraction of 4% and the LS solution with the mass fraction of 10% according to the following weight ratio of 1:1 blending, adding a cross-linking agent ethylene glycol diglycidyl ether (EDGE), wherein the EDGE dosage is 50% of the dry weight of HEC and LS in the mixture, and mixing for 0.5h at 80 ℃ to obtain an HEC/LS blend; finally, freeze-drying the HEC/LS mixture for 48 hours in a freeze dryer, repeatedly washing with deionized water to remove sodium hydroxide in the composite material, then placing the composite material into a refrigerator at the temperature of minus 20 ℃ for 24 hours, and finally obtaining the composite cellulose/luffa aerogel after freeze drying, which is named HEC/LS;
step four, preparing microorganism Bn-HEC/LS aerogel
Sterilizing the HEC/LS aerogel obtained in the step three in an autoclave at 121 ℃ for 30 minutes for later use;
HEC/LS aerogel immobilized microorganisms are divided into three steps: firstly, enabling HEC/LS sponge to completely adsorb 3% PVA solution, removing redundant PVA through filtration, and preventing the internal pores of aerogel from being blocked; secondly, immersing the HEC/LS sponge into the mixed microorganism suspension obtained in the first step until the adsorption is saturated, and drying for 1 hour at 30 ℃, wherein the mixing ratio of BTS to NL to PO to PA to AL in the mixed microorganism suspension is 1:1:1:1; repeating the steps for a plurality of times until the adsorption reaches saturation; finally, ca (NO) 3 ) 2 The solution is subjected to a crosslinking reaction at 0-4deg.C for 30min, dried at 30deg.C for 1h, and the excess Ca (NO) is filtered off with suction 3 ) 2 A solution; repeating the operation for 5 times, wherein the carriers after the 1 st, 2 nd, 3 rd, 4 th and 5 th times of microorganism loading are respectively named as B1-HEC/LS, B2-HEC/LS, B3-HEC/LS, B4-HEC/LS and B5-HEC/LS;
step five, preparation of oleophylic and hydrophobic high-activity loaded microorganism Bn-HEC/LS aerogel
Taking the material (B5-HEC/LS) loaded with the petroleum hydrocarbon degrading bacteria for 5 times in the fourth step as a subsequent oleophylic and hydrophobic modified material; firstly, adding polyvinylidene fluoride (PVDF) into N, N Dimethylformamide (DMF) solution, and stirring for 1h at normal temperature to fully mix the polyvinylidene fluoride (PVDF); then, the B5-HEC/LS sponge is quickly immersed into the mixed solution; finally, the material immersed in the mixed solution is quickly taken out and put into distilled water to form a hydrophobic coating on the surface. The resulting hydrophobically oleophilic modified material was designated OB5-HEC/LS aerogel.
2. The method for preparing the oleophilic hydrophobic high-activity microorganism-loaded composite cellulose/luffa aerogel according to claim 1, which is characterized by comprising the following steps: in the third step, the repeated operation is performed 10 times.
3. The method for preparing the oleophilic hydrophobic high-activity microorganism-loaded composite cellulose/luffa aerogel according to claim 1, which is characterized by comprising the following steps: in the third step, the LS solution is a viscous solution with LS content of 10%, and the mass of the cross-linking agent ethylene glycol diglycidyl ether (EDGE) is 7g.
4. The method for preparing the oleophilic hydrophobic high-activity microorganism-loaded composite cellulose/luffa aerogel according to claim 1, which is characterized by comprising the following steps: in the fourth step, the repeated operation is performed 4 times.
5. The method for preparing the oleophilic hydrophobic high-activity microorganism-loaded composite cellulose/luffa aerogel according to claim 1, which is characterized by comprising the following steps: in the fourth step, quantitative bacterial suspension is added into the HEC/LS, and the 1 st, 2 nd, 3 rd, 4 th and 5 th times of microorganism loading amounts are respectively as follows: 5.5853g, 8.2214g, 10.8963g, 12.46996g, 13.3521g.
6. The method for preparing the oleophilic hydrophobic high-activity microorganism-loaded composite cellulose/luffa aerogel according to claim 1, which is characterized by comprising the following steps: in step four, the Ca (NO 3 ) 2 The concentration of (2) was 5% and the amount added was 20mL.
7. The method for preparing the oleophilic hydrophobic high-activity microorganism-loaded composite cellulose/luffa aerogel according to claim 1, which is characterized by comprising the following steps: in the fifth step, the ratio of PVDF to DMF in the hydrophobically modified reagent is 1:10.
8. the oleophilic hydrophobic high-activity microorganism-loaded composite cellulose/luffa aerogel prepared by the preparation method according to any one of claims 1-7.
9. The use of the oleophilic hydrophobic high-activity loaded microorganism composite cellulose/luffa aerogel as claimed in claim 8 in degrading diesel oil.
10. The use according to claim 9, characterized in that: the substrate was a mixture of 1% diesel and 30mL deionized water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310756731.8A CN116875586B (en) | 2023-06-26 | 2023-06-26 | Preparation method and application of oleophylic and hydrophobic high-activity microorganism-loaded composite cellulose/luffa aerogel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310756731.8A CN116875586B (en) | 2023-06-26 | 2023-06-26 | Preparation method and application of oleophylic and hydrophobic high-activity microorganism-loaded composite cellulose/luffa aerogel |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116875586A true CN116875586A (en) | 2023-10-13 |
| CN116875586B CN116875586B (en) | 2024-08-23 |
Family
ID=88265315
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310756731.8A Active CN116875586B (en) | 2023-06-26 | 2023-06-26 | Preparation method and application of oleophylic and hydrophobic high-activity microorganism-loaded composite cellulose/luffa aerogel |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116875586B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109137133A (en) * | 2018-08-20 | 2019-01-04 | 安徽工程大学 | A kind of preparation method of loofah fiber element/chitosan composite fiber |
| CN109482638A (en) * | 2018-12-14 | 2019-03-19 | 中国石油集团川庆钻探工程有限公司 | Pseudomonas oil-reducing bacterium L-1, mixed microbial inoculum thereof and in-situ remediation method for petroleum-polluted soil |
| CN112480464A (en) * | 2020-11-19 | 2021-03-12 | 华南理工大学 | Hydrophobic lignin/cellulose aerogel oil-water separation material and preparation method and application thereof |
| WO2021069783A1 (en) * | 2019-10-07 | 2021-04-15 | Consejo Superior De Investigaciones Científicas | Method for preparing hydrophobic aerogels |
| CN113373136A (en) * | 2021-05-20 | 2021-09-10 | 西北民族大学 | Preparation method and application of oleophylic hydrophobic high-activity microorganism-loaded sunflower disc porous material |
| CN113577636A (en) * | 2021-07-29 | 2021-11-02 | 西北民族大学 | Preparation and application of oleophylic hydrophobic high-activity loaded microorganism asphalt porous material |
-
2023
- 2023-06-26 CN CN202310756731.8A patent/CN116875586B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109137133A (en) * | 2018-08-20 | 2019-01-04 | 安徽工程大学 | A kind of preparation method of loofah fiber element/chitosan composite fiber |
| CN109482638A (en) * | 2018-12-14 | 2019-03-19 | 中国石油集团川庆钻探工程有限公司 | Pseudomonas oil-reducing bacterium L-1, mixed microbial inoculum thereof and in-situ remediation method for petroleum-polluted soil |
| WO2021069783A1 (en) * | 2019-10-07 | 2021-04-15 | Consejo Superior De Investigaciones Científicas | Method for preparing hydrophobic aerogels |
| CN112480464A (en) * | 2020-11-19 | 2021-03-12 | 华南理工大学 | Hydrophobic lignin/cellulose aerogel oil-water separation material and preparation method and application thereof |
| CN113373136A (en) * | 2021-05-20 | 2021-09-10 | 西北民族大学 | Preparation method and application of oleophylic hydrophobic high-activity microorganism-loaded sunflower disc porous material |
| CN113577636A (en) * | 2021-07-29 | 2021-11-02 | 西北民族大学 | Preparation and application of oleophylic hydrophobic high-activity loaded microorganism asphalt porous material |
Non-Patent Citations (2)
| Title |
|---|
| HANG YANG等: "Preparation of hydrophobic carbon aerogel using cellulose extracted from luffa sponge for adsorption of diesel oil", CERAMICS INTERNATIONAL, vol. 47, no. 23, 1 December 2021 (2021-12-01), pages 33827 - 33834, XP086831069, DOI: 10.1016/j.ceramint.2021.08.294 * |
| YIHUAN WANG等: "Construction of Natural Loofah/Poly(vinylidene fluoride) Core–Shell Electrospun Nanofibers via a Controllable Janus Nozzle for Switchable Oil–Water Separation", ACS APPLIED MATERIALS & INTERFACES, vol. 12, no. 46, 4 November 2020 (2020-11-04), pages 51917 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN116875586B (en) | 2024-08-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104475444A (en) | Preparation, application and usage method for biocarbon-immobilized composite pollution-degrading bacterium particle | |
| CN101134955A (en) | Solid composite micro-organism micro-balloon for organic contaminant biodegradation and method for preparing the same | |
| CN1788869A (en) | Solid composite microbe microsphere for soil rehabilitation and its preparation method | |
| CN113215009B (en) | Composite immobilized microbial agent and preparation method and application thereof | |
| Rezaee et al. | Microbial cellulose as support material for the immobilization of denitrifying bacteria. | |
| CN108017793A (en) | A kind of application being sustained in the preparation method and its chemical wastewater treatment of polyurethane mesh carrier | |
| CN107151663B (en) | Immobilized microbial agent prepared from kelp residue and used for petroleum pollution remediation | |
| Rezaee et al. | Biological denitrification by Pseudomonas stutzeri immobilized on microbial cellulose | |
| JP4092592B2 (en) | Heating carrier, method for producing the same, and environmental purification method using heating carrier | |
| KR101511999B1 (en) | Method for improving water quality and capsule for improving water quality used in the method | |
| CN116875586B (en) | Preparation method and application of oleophylic and hydrophobic high-activity microorganism-loaded composite cellulose/luffa aerogel | |
| CN106754513B (en) | Preparation and application of TX-100 modified sodium alginate embedded pseudomonas particles | |
| CN108975494B (en) | Method for treating black smelly water by using graphene modified straw material | |
| RU2405741C2 (en) | Cartridge for purifying natural water from oil pollutants | |
| CN113577636B (en) | Preparation and application of oleophylic hydrophobic high-activity loaded microorganism asphalt porous material | |
| CN114958669B (en) | Michigan klebsiella and product and application thereof | |
| RU2327649C2 (en) | Method of biological preparation produced for restoration of oil-polluted water reservoirs | |
| CN113373136B (en) | Preparation method and application of porous material of oleophilic hydrophobic high-activity microorganism-loaded sunflower disc | |
| Siripattanakul-Ratpukdi et al. | Municipal wastewater treatment using barium alginate entrapped activated sludge: adjustment of utilization conditions | |
| CN112058290B (en) | Application of photocatalysis modified material in removing marine spilled oil | |
| JPH07505336A (en) | Apparatus and method for removing pollutants from wastewater | |
| CN110564716B (en) | Bacterium-carrying composite microsphere for synchronously removing phenol and aniline, and preparation method and application thereof | |
| CN111875066A (en) | Immobilized microorganism particles for degrading grease in kitchen wastewater, preparation method and application | |
| CN113502247A (en) | Composite functional microbial inoculum for removing total nitrogen from high-salinity wastewater and application thereof | |
| CN111498987A (en) | Domestic sewage in-situ purification immobilized carrier and microbial inoculum loading method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |