CN116875452A - Pepsin enzymolysis tank - Google Patents

Pepsin enzymolysis tank Download PDF

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Publication number
CN116875452A
CN116875452A CN202311126788.6A CN202311126788A CN116875452A CN 116875452 A CN116875452 A CN 116875452A CN 202311126788 A CN202311126788 A CN 202311126788A CN 116875452 A CN116875452 A CN 116875452A
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Prior art keywords
end wall
ring seat
communicated
tank body
tube
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Granted
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CN202311126788.6A
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CN116875452B (en
Inventor
余美伦
秦香芹
朱佳伟
刘维刚
李晓龙
姜璐璐
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Harbin Hanbang Medical Science And Technology Co ltd
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Harbin Hanbang Medical Science And Technology Co ltd
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Priority to CN202311126788.6A priority Critical patent/CN116875452B/en
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    • C12M21/18Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
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    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06Nozzles; Sprayers; Spargers; Diffusers
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    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
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    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/26Conditioning fluids entering or exiting the reaction vessel
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    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18Heat exchange systems, e.g. heat jackets or outer envelopes
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    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure

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Abstract

The application provides a pepsin enzymolysis tank applied to the pepsin enzymolysis field, which circularly pumps pepsin enzymolysis reaction substances put into a main tank body through a pump, and a plurality of annular spray pipes are concentrically arranged on the same plane outside an annular seat from inside to outside so as to be communicated with a water outlet of the pump, and the annular spray pipes are matched with annular spray ports formed at symmetrical positions at the bottom of the annular spray pipes in a surrounding manner, and the annular seat slowly lifts, so that the reaction substances sprayed back can form corrugated surfaces which slowly lift up and down in the reaction substances in the main tank body, the contact comprehensiveness of pepsin and other substances in the reaction substances is improved under the condition of not causing distance collision, the enzymolysis reaction effect is improved to a certain extent, and the condition of reducing the pepsin activity can be avoided.

Description

Pepsin enzymolysis tank
Technical Field
The application relates to the field of pepsin enzymolysis, in particular to a pepsin enzymolysis tank.
Background
The pepsin enzymolysis tank is a reaction device for pepsin enzymolysis, and the pepsin enzymolysis reaction can decompose protein into peptide, tyrosine, phenylalanine and other amino acids, so that the pepsin enzymolysis tank has wide application in the biopharmaceutical industry.
In order to accelerate the rate of pepsin enzymolysis reaction, the enzymolysis tank in the prior art is generally provided with a stirring structure inside the enzymolysis tank, for example, publication No. CN206188808U discloses a pepsin enzymolysis device, which is arranged in the first-stage enzymolysis tank by installing a stirring mechanism, and the rate of the enzymolysis reaction in the first-stage enzymolysis tank is enhanced by means of the rotation of stirring blades arranged in the stirring mechanism, but in the actual operation process, the direct stirring can cause the reaction substances in the enzymolysis tank to generate violent chaotic collision, and the violent stirring can enable the reaction substances in the enzymolysis tank to be uniformly mixed with each other with high efficiency, but pepsin can reduce the activity when being subjected to the violent collision caused by the violent stirring, so that the enzymolysis effect of the pepsin in the enzymolysis tank is laterally influenced.
Disclosure of Invention
Compared with the prior art, the pepsin enzymolysis tank provided by the application comprises a main tank body, a transfer tank, a pump, a return pipe I, a shaft tube and a ring seat, wherein the transfer tank is fixedly arranged at the bottom axial center position of the main tank body; the pump is fixedly arranged on the outer end wall of the main tank body, and the water outlet of the pump is communicated with the bottom of the switching tank; the back suction pipe is communicated between the water inlet of the pump and the inside of the main tank body; the shaft tube is vertically arranged at the inner axis position of the main tank body; the ring seat capable of slowly lifting is sleeved on the outer side of the shaft tube, and is communicated with the transfer pot; the annular spray pipes are sequentially sleeved on the outer sides of the annular seats in a concentric mode, the annular spray pipes are communicated with the annular seats through radial through pipes, and the annular spray pipes are symmetrically arranged on the bottom end walls of the annular spray pipes in a surrounding mode.
Further, a second vertically arranged drawing pipe is fixedly arranged on the inner end wall of the main tank body from top to bottom, an inner thread sleeve connected to the bottom of the ring seat is sleeved on the outer side thread sleeve of the shaft pipe, a clamping rod which is clamped on the outer side of the drawing pipe in a sliding manner is fixedly arranged on the outer end wall of the inner thread sleeve, a motor for driving the shaft pipe to slowly rotate is fixedly arranged on the top of the main tank body, and the motor is electrically connected with the control panel.
Further, the ring seat is rotationally connected with the internal thread sleeve, a chute which is vertically arranged is arranged on the outer end wall of the shaft tube, a slide block which is matched with the inner size of the chute is fixedly arranged on the inner end wall of the ring seat, and the slide block is arranged in the chute in a sliding way.
Further, the bottom of central siphon rotates to communicate in the transfer jar inside, and the spout directly link up with the central siphon is inside, fixedly connected with on the slider is linked together with the ring seat inside pipe, and the pipe runs through the spout and extends to the central siphon inside, and the internally mounted of central siphon has flexible pipe, and the bottom outer end wall of flexible pipe is sealed fixed with the bottom inner end wall of central siphon, and the pipe is fixed to communicate with the top of flexible pipe.
Further, the inner end wall of the chute is fixedly provided with adhesive tapes symmetrically clamped on the outer side of the circular pipe, and two adhesive tapes in the same chute are tightly attached.
Further, a cylinder which vertically extends to the inside of the main tank body is fixedly arranged at the axial center position of the top of the main tank body, a ceramic plate is hermetically arranged at the middle position inside the cylinder, a first magnetic disk and a second magnetic disk which are positioned on the upper side and the lower side of the ceramic plate are rotatably arranged inside the cylinder, the first magnetic disk is magnetically connected with the second magnetic disk, the axial center of the first magnetic disk is fixedly connected with a driving shaft of a motor, and the axial center of the second magnetic disk is fixedly connected with the top end of the shaft tube.
Further, a circular pipe which is arranged in a surrounding manner is fixedly arranged at the lower position of the inner end wall of the main tank body, holes which are distributed in a surrounding manner are uniformly formed in the bottom end wall of the circular pipe, and the second return suction pipe is communicated between the first return suction pipe and the circular pipe.
Further, the top fixed mounting of ring seat has the frame shell of vertical setting, and the inside top fixed mounting of frame shell has pressure sensor, and the inside movable mounting of frame shell has the floater that is located pressure sensor below, and touch switch is installed equally to the bottom of ring seat, and pressure sensor and touch switch all with control panel electric connection.
Further, the outer end wall of the main tank body is fixedly provided with a heat preservation tank, and the first back suction pipe is spirally arranged inside the heat preservation tank.
Further, the electric heating module and the temperature control switch are respectively and fixedly arranged in the heat preservation tank, and are electrically connected with the control panel.
Compared with the prior art, the application has the advantages that:
(1) According to the application, the pepsin enzymolysis reaction substances put into the main tank body are circularly pumped by the pump, the plurality of annular spray pipes are concentrically arranged on the same plane outside the annular seat from inside to outside, so that the annular spray pipes are communicated with the water outlet of the pump, the annular spray pipes are circumferentially arranged at symmetrical positions at the bottom of the annular spray pipes, and the annular seat slowly lifts, so that the reaction substances sprayed by the back pumping form a corrugated surface which slowly lifts up and down and is positioned in the reaction substances in the main tank body, the contact comprehensiveness of pepsin and other substances in the reaction substances is improved under the condition of not causing distance collision, the enzymolysis reaction effect is improved to a certain extent, and the condition of reducing the pepsin activity can be avoided.
(2) Through with the spout vertical seting up on the outer end wall of central siphon to will with the slider fixed mounting of spout looks adaptation inside the ring seat, with the help of the cooperation of slider and spout, make the ring seat go up and down the in-process can follow central siphon synchronous slow rotation, and then make the ripple face that the back spouts the constitution slowly go up and down the in-process can also slowly rotate, be favorable to further strengthening the comprehensive sufficiency that the inside pepsin of reaction material contacted with other materials.
(3) Through sealing the intermediate position of fixing at the drum with the ceramic plate, realize the rotary drive of motor to the central siphon with the help of the magnetism of magnetism dish one and magnetism dish two for both ends are sealed each other not UNICOM about the drum, when guaranteeing motor drive central siphon rotation stability, can also effectively ensure the whole sealed stability of main jar body.
(4) Through the frame shell fixed mounting with internally mounted pressure sensor at the top of ring seat to with the floater movable mounting that can trigger pressure sensor inside the frame shell, can be when the ring seat is about to break away from the interior reactant liquid level of main jar, send relevant signal to control panel, control ring seat switching-over decline, the cooperation is installed and is pressed the switch in ring seat bottom, be favorable to guaranteeing ring seat and the annular spray tube of outside and remain all the time in the interior reactant of main jar, be favorable to avoiding the reaction material in the back spraying process to rise to the interior reactant liquid level of main jar and spout and cause violent vibration collision, be favorable to fully guaranteeing the activity of pepsin in the enzymolysis reaction process.
(5) Through setting up the return pipe one spiral in the heat preservation jar to pour 37 ℃ warm water into the heat preservation jar, make the pepsin that flows through in the reaction material of return pipe one in the return process can be in 37 ℃ suitable temperature, be favorable to arousing pepsin's activity, thereby effectively promote enzymolysis reaction efficiency.
Drawings
FIG. 1 is a front cross-sectional view of the overall structure of the present application;
FIG. 2 is a schematic diagram of the structure at A in FIG. 1;
FIG. 3 is a schematic diagram of the structure at B in FIG. 1;
FIG. 4 is a perspective view of the overall structure of the present application;
FIG. 5 is a top cross-sectional view of the overall structure of the present application;
FIG. 6 is a schematic view of the structure at C in FIG. 5;
FIG. 7 is a perspective view of the bottom of the split ring seat and female threaded sleeve of the present application;
FIG. 8 is a split view of the internal structure of the cylinder of the present application;
FIG. 9 is a perspective view of a frame shell of the present application;
fig. 10 is a perspective view showing the internal structure of the insulation can of the present application.
The reference numerals in the figures illustrate: 1. a main tank body; 101. a transfer pot; 102. a pump; 103. a first back suction pipe; 104. a grommet; 105. a second back suction pipe; 2. a shaft tube; 201. a ring seat; 202. an annular nozzle; 203. an annular nozzle; 204. an internal thread sleeve; 205. a clamping rod; 206. a chute; 207. a slide block; 208. a round tube; 209. a telescopic tube; 210. an adhesive tape; 3. a motor; 4. a cylinder; 401. a ceramic plate; 402. a first magnetic disk; 403. a second magnetic disk; 5. a frame shell; 501. a pressure sensitive sensor; 502. a floating ball; 6. a heat preservation tank; 601. an electric heating module; 602. a temperature control switch.
Detailed Description
The embodiments of the present application will be described in detail and fully with reference to the accompanying drawings, and it is intended that all other embodiments of the application, which are apparent to one skilled in the art without the inventive faculty, are included in the scope of the present application.
Embodiment one:
the application provides a pepsin enzymolysis tank, referring to fig. 1-10, which comprises a main tank body 1, wherein an adapter tank 101 is fixedly arranged at the bottom axial center position of the main tank body 1, a pump 102 is fixedly arranged on the outer end wall of the main tank body 1, a first back suction pipe 103 is communicated between the water inlet of the pump 102 and the inside of the main tank body 1, a water outlet of the pump 102 is fixedly communicated with the bottom of the adapter tank 101, a vertical shaft tube 2 is arranged at the inner axial center position of the main tank body 1, a slowly lifting ring seat 201 is sleeved on the outer side of the shaft tube 2, a plurality of annular spray pipes 202 concentrically arranged in the main tank body 1 are sequentially sleeved on the outer side of the ring seat 201, a plurality of annular spray pipes 202 are communicated with the ring seat 201 through radially arranged through pipes, annular spray pipes 203 with symmetrical left and right positions are arranged on the bottom end wall of the annular spray pipes 202, the inside of the ring seat 201 is communicated with the adapter tank 101, a control panel is fixedly arranged on the outer end wall of the main tank body 1, and the pump 102 is electrically connected with the control panel.
In this embodiment, be equipped with the external screw thread on the outer end wall of central siphon 2, can set up the business turn over mouth of pipe, throw material mouth and visual window scheduling adaptation structure on the main jar body 1 according to the user demand, in this enzymolysis tank use, the staff connects external power supply with this device for external power supply provides electric power support for this device, afterwards, the staff can start the use through control panel control device, the staff drops into the main jar body 1 inside with pepsin enzymolysis reaction substance, at this moment, install the pump 102 circular telegram start in the main jar body 1 outside, circulate the enzymolysis reaction substance of throwing into in the main jar body 1.
Specifically, the reaction substance is pumped out of the main tank 1 through the first pumping pipe 103, enters the transfer tank 101 through the pumping of the pump 102, and enters the annular seat 201 due to the fact that the annular seat 201 is communicated with the transfer tank 101, then enters the annular spraying pipe 202 communicated with the annular seat 201, is slowly sprayed out through the annular spraying holes 203 formed in the annular spraying pipe 202, and flows back into the main tank 1 again, and due to the fact that the annular spraying holes 203 are formed in symmetrical positions of the bottom end walls of the annular spraying pipes 202, the annular spraying pipes 202 are concentrically arranged from inside to outside, and the backflow reaction substance sprayed out through the annular spraying holes 203 on the annular spraying pipes 202 is in a corrugated shape.
Further, the cooperation ring seat 201 can follow central siphon 2 and slowly reciprocate for the ripple face that backward flow reactant constitutes can be in the inside oscilaltion of the reactant in main jar body 1, has not only effectively strengthened the comprehensive sufficiency of contact between the reactant, can also avoid the too violent collision impact of reactant, thereby effectively avoid pepsin in the reactant to receive violent collision and activity reduction, is favorable to guaranteeing pepsin at the high-efficient stability of main jar body 1 enzymolysis reaction.
Referring to fig. 5 and 7, a second vertically disposed drawing tube 105 is fixedly mounted on an inner end wall of the main tank 1 from top to bottom, an inner threaded sleeve 204 connected to the bottom of the ring seat 201 is sleeved on an outer thread of the shaft tube 2, a clamping rod 205 slidably clamped on the outer side of the second drawing tube 105 is fixedly mounted on an outer end wall of the inner threaded sleeve 204, a motor 3 for driving the shaft tube 2 to slowly rotate is fixedly mounted on the top of the main tank 1, and the motor 3 is electrically connected with a control panel.
At this time, the clamping rod 205 is fixedly installed on the outer end wall of the inner threaded sleeve 204 and is in up-and-down sliding clamping with the second vertically arranged drawing pipe 105, so that the inner threaded sleeve 204 can be driven to move up and down in the rotation process of the shaft pipe 2 by means of the threaded connection between the inner threaded sleeve 204 and the shaft pipe 2, and the ring seat 201 is connected with the inner threaded sleeve 204, so that the up-and-down lifting of the ring seat 201 is driven, the driving of the motor 3 on the shaft pipe 2 is controlled to be reversed, the synchronous control on the lifting direction of the ring seat 201 is realized, and the operation is convenient and simple.
Embodiment two:
referring to fig. 2 and 7, the ring seat 201 is rotationally connected with the internal thread sleeve 204, a vertically disposed sliding groove 206 is formed in the outer end wall of the shaft tube 2, a sliding block 207 adapted to the inner dimension of the sliding groove 206 is fixedly mounted on the inner end wall of the ring seat 201, and the sliding block 207 is slidably disposed inside the sliding groove 206.
It should be noted that, during the process of the retarded rotation of the ring seat 201, the plurality of ring-shaped spray pipes 202 coaxially sleeved on the outer side of the ring seat are driven to rotate synchronously, so that the corrugated surface formed by the backflow and spraying of the reactant can not only slowly rise and fall, but also slowly rotate along with the rotation of the shaft tube 2, further enhancing the complete and sufficient contact between pepsin and other substances in the reactant, and further improving the reaction efficiency.
Referring to fig. 1 and 2, the bottom end of the shaft tube 2 is rotatably connected to the inside of the transfer pot 101, the chute 206 is directly communicated with the inside of the shaft tube 2, the sliding block 207 is fixedly connected with a circular tube 208 communicated with the inside of the ring seat 201, the circular tube 208 extends to the inside of the shaft tube 2 through the chute 206, the telescopic tube 209 is installed in the inside of the shaft tube 2, the outer end wall of the bottom of the telescopic tube 209 is sealed and fixed with the inner end wall of the bottom of the shaft tube 2, the circular tube 208 is fixedly communicated with the top end of the telescopic tube 209, during the use of the enzymolysis pot, the reaction substance pumped by the pump 102 enters the transfer pot 101, enters the inside of the ring seat 201 through the communication of the circular tube 208 and the telescopic tube 209, and the telescopic tube 209 follows the synchronous telescopic operation during the lifting of the ring seat 201 along the shaft tube 2, so as to ensure the stability of the reaction substance flowing back to the inside of the ring seat 201.
Referring to fig. 2 and 7, the inner end wall of the chute 206 is fixedly provided with the adhesive tapes 210 symmetrically clamped on the outer side of the circular tube 208, and the two adhesive tapes 210 in the same chute 206 are tightly attached, so that in the use process of the enzymolysis tank, the adhesive tapes 210 which are tightly attached are symmetrically arranged on the inner end wall of the chute 206 from top to bottom, so that the circular tube 208 is ensured to stably lift up and down along the chute 206 along with the shaft tube 2, and meanwhile, excessive reaction substances can be prevented from entering the shaft tube 2, thereby being beneficial to ensuring the stability of the enzymolysis tank in the operation process.
Referring to fig. 1 and 8, a cylinder 4 extending vertically into the main tank 1 is fixedly mounted at the top axial center of the main tank 1, a ceramic plate 401 is mounted in a sealing manner in the middle of the inside of the cylinder 4, a first magnetic disk 402 and a second magnetic disk 403 are rotatably mounted in the cylinder 4 and located on the upper side and the lower side of the ceramic plate 401, the first magnetic disk 402 and the second magnetic disk 403 are magnetically connected, the axial center of the first magnetic disk 402 is fixedly connected with a driving shaft of the motor 3, and the axial center of the second magnetic disk 403 is fixedly connected with the top end of the shaft tube 2.
In the use process of the enzymolysis tank, the motor 3 is electrified and started to drive the first magnetic disk 402 fixedly connected with the driving shaft thereof to rotate, the second magnetic disk 403 is synchronously rotated along with the first magnetic disk 402 by virtue of the magnetic connection between the first magnetic disk 402 and the second magnetic disk 403, so that the rotary driving of the shaft tube 2 is realized, and the upper end and the lower end of the cylinder 4 are not communicated with each other due to the fact that the ceramic plate 401 is isolated between the first magnetic disk 402 and the second magnetic disk 403, so that the integral sealing stability of the main tank body 1 between transmission connection is ensured when the motor 3 drives the shaft tube 2.
Referring to fig. 1, a loop 104 is fixedly installed at a position below an inner end wall of a main tank body 1, holes distributed in a surrounding manner are uniformly formed in a bottom end wall of the loop 104, a second drawing pipe 105 is communicated between a first drawing pipe 103 and the loop 104, and during use of the enzymolysis tank, under the pumping action of a pump 102, enzymolysis reaction substances in the main tank body 1 enter the loop 104 through the holes in the bottom end wall of the loop 104, and are pumped into the pump 102 through the communication of the second drawing pipe 105 and the first drawing pipe 103, and as the loop 104 serving as a suction end is arranged at a position below the inner part of the main tank body 1, the continuous stability of the pump 102 on the extraction of the reaction substances in the main tank body 1 can be ensured, and the holes are distributed on the loop 104 in a circular ring structure in a surrounding manner, so that the reaction substances can be more comprehensively and uniformly extracted at the bottom of the main tank body 1.
Referring to fig. 2 and 7, a frame shell 5 is fixedly mounted on top of a ring seat 201, a pressure sensor 501 is fixedly mounted above the inside of the frame shell 5, a floating ball 502 is movably mounted inside the frame shell 5 and below the pressure sensor 501, a touch switch is also mounted on the bottom of the ring seat 201, and the pressure sensor 501 and the touch switch are electrically connected with a control panel.
During the use process of the enzymolysis tank, when the ring seat 201 moves upwards to move out the liquid level of the reaction substance in the main tank body 1, the frame shell 5 firstly stretches out of the liquid level of the reaction substance, when the frame shell 5 stretches out of the liquid level of the reaction substance, the buoyancy provided by the reaction substance to the floating ball 502 is lost, so that the floating ball 502 falls down in the frame shell 5, at the moment, the contact pressure signal of the floating ball 502 to the pressure sensor 501 is lost, the pressure sensor 501 transmits the relevant signal into the control panel, the control panel controls the motor 3 to switch the driving direction of the shaft tube 2, and the ring seat 201 is controlled to move downwards.
When the ring seat 201 moves downwards to the lowest position, the touch switch arranged at the bottom of the ring seat 201 is extruded and synchronously transmits related signals to the control panel, the driving direction of the shaft tube 2 is switched again through the control panel control motor 3, so that the ring seat 201 drives the annular spray tube 202 to move upwards, the operation is repeatedly performed, the annular spray tube 202 is ensured to be carried by the ring seat 201 to always lift up and down in the reaction substance in the main tank body 1, the reaction substance liquid level in the main tank body 1 is prevented from being separated in the rising process of the annular spray tube 202, and severe oscillation collision caused by the reaction substance in the back spraying process is avoided on the reaction substance liquid level in the main tank body 1, so that the activity of pepsin in the enzymolysis reaction process is fully ensured.
Referring to fig. 1, a heat preservation tank 6 is fixedly installed on an outer end wall of a main tank body 1, a first pumping pipe 103 is spirally arranged inside the heat preservation tank 6, warm water at 37 ℃ is poured into the heat preservation tank 6, and in the use process of the enzymolysis tank, a water inlet valve and a water outlet valve for the warm water to enter and exit are arranged on the heat preservation tank 6, when a reaction substance flows through the inside of the first pumping pipe 103, the warm water at 37 ℃ in the heat preservation tank 6 can provide optimal enzymolysis environment temperature for pepsin flowing through the reaction substance, the activity of the pepsin is ensured, and the total length of the inside of the first pumping pipe 103 in a spiral shape can be greatly enhanced, so that the time length of contact with the warm water at 37 ℃ in the heat preservation tank 6 during the back pumping of the reaction substance is prolonged, and the enzymolysis efficiency of the reaction substance when the reaction substance flows back into the main tank body 1 is improved.
Referring to fig. 1 and 10, an electric heating module 601 and a temperature control switch 602 are fixedly installed inside a heat preservation tank 6, the upper temperature control limit of the temperature control switch 602 is set to 40 ℃, the lower temperature control limit of the temperature control switch 602 is set to 35 ℃, the electric heating module 601 and the temperature control switch 602 are electrically connected with a control panel, in the use process of the enzymolysis tank, the electric heating module 601 installed inside the heat preservation tank 6 is electrified and started to heat warm water added into the heat preservation tank 6, the temperature of the warm water is monitored in real time by the temperature control switch 602 installed inside the heat preservation tank 6, when the temperature of the warm water reaches 40 ℃, the electric heating module 601 is closed to stop heating, when the temperature of the warm water is reduced to 35 ℃, the electric heating module 601 is controlled to be electrified and started, the warm water in the heat preservation tank 6 is continuously heated, so that the temperature of the warm water in the heat preservation tank 6 is controlled to be in the range of 35-40 ℃, and long-term stable activity excitation of reactive substances flowing in the first pumping-back pipe 103 is realized from the aspect of environmental temperature maintenance.
Working principle:
the first step: the staff puts pepsin enzymolysis reaction substances into the main tank 1, fills warm water at 37 ℃ into the heat preservation tank 6, starts the motor 3 to drive the shaft tube 2 to rotate, drives the ring seat 201 to slowly lift up and down in the reaction substances in the main tank 1 along the shaft tube 2, and synchronously and slowly rotates;
and a second step of: the pump 102 is electrified and started, the reaction substance in the main tank body 1 enters the first pumping pipe 103 through the communication of the annular pipe 104 and the second pumping pipe 105, pepsin in the reaction substance flowing through the first pumping pipe 103 is activated through warm water at 37 ℃, and then is slowly sprayed out through the annular nozzles 203 at the bottoms of the annular spray pipes 202 concentrically arranged on the same plane through the communication of the transfer tank 101, the telescopic pipe 209, the circular pipe 208, the annular seat 201 and the through pipe to form a sector surface, and fully and uniformly contacts with the reaction substance in the main tank body 1 to accelerate the reaction efficiency.
The present application is not limited to the preferred embodiments described above, but is merely preferred for practical application.

Claims (6)

1. The utility model provides a pepsin enzymolysis tank, includes main jar body (1), transfer jar (101), pump (102), back suction pipe one (103), central siphon (2), ring seat (201), annular spray tube (202) and annular spout (203), its characterized in that:
the transfer tank (101) is fixedly arranged at the bottom axle center position of the main tank body (1);
the pump (102) is fixedly arranged on the outer end wall of the main tank body (1), and the water outlet of the pump (102) is communicated with the bottom of the transfer tank (101);
the first back suction pipe (103) is communicated between the water inlet of the pump (102) and the inside of the main tank body (1);
the shaft tube (2) is vertically arranged at the inner axis position of the main tank body (1);
the ring seat (201) capable of slowly lifting is sleeved on the outer side of the shaft tube (2), and the ring seat (201) is communicated with the transfer pot (101);
the annular spray pipes (202) are sequentially and concentrically sleeved on the outer side of the annular seat (201), the annular spray pipes (202) are communicated with the annular seat (201) through radial through pipes, and the annular spray pipes (203) are symmetrically and circumferentially arranged on the bottom end wall of the annular spray pipes (202);
the inner end wall of the main tank body (1) is fixedly provided with a second drawing pipe (105) which is vertically arranged from top to bottom, the outer side thread of the shaft pipe (2) is sleeved with an inner thread sleeve (204) connected to the bottom of the ring seat (201), the outer end wall of the inner thread sleeve (204) is fixedly provided with a clamping rod (205) which is clamped on the outer side of the second drawing pipe (105) in a sliding manner, and the top of the main tank body (1) is fixedly provided with a motor (3) for driving the shaft pipe (2) to slowly rotate;
the ring seat (201) is rotationally connected with the internal thread sleeve (204), a chute (206) which is vertically arranged is formed in the outer end wall of the shaft tube (2), and a sliding block (207) which is matched with the inner size of the chute (206) is fixedly arranged on the inner end wall of the ring seat (201);
the bottom end of the shaft tube (2) is rotationally communicated with the inside of the transfer pot (101), the sliding groove (206) is directly communicated with the inside of the shaft tube (2), a round tube (208) communicated with the inside of the ring seat (201) is fixedly connected to the sliding block (207), the round tube (208) penetrates through the sliding groove (206) and extends to the inside of the shaft tube (2), a telescopic tube (209) is arranged in the shaft tube (2), the outer end wall of the bottom of the telescopic tube (209) is fixed with the inner end wall of the bottom of the shaft tube (2) in a sealing manner, and the round tube (208) is fixedly communicated with the top end of the telescopic tube (209);
the circular pipe (104) which is arranged in a surrounding mode is fixedly arranged at the position below the inner end wall of the main tank body (1), holes which are distributed in a surrounding mode are uniformly formed in the bottom end wall of the circular pipe (104), and the second return suction pipe (105) is communicated between the first return suction pipe (103) and the circular pipe (104).
2. The pepsin enzymolysis tank according to claim 1, wherein: the inner end wall of the chute (206) is fixedly provided with adhesive tapes (210) symmetrically clamped on the outer side of the circular tube (208), and the two adhesive tapes (210) in the same chute (206) are tightly attached.
3. The pepsin enzymolysis tank according to claim 1, wherein: the novel ceramic pot is characterized in that a cylinder (4) which vertically extends to the inside of the main pot body (1) is fixedly arranged at the top axis position of the main pot body (1), a ceramic plate (401) is hermetically arranged at the middle position inside the cylinder (4), a first magnetic disc (402) and a second magnetic disc (403) which are positioned on the upper side and the lower side of the ceramic plate (401) are rotatably arranged inside the cylinder (4), and the first magnetic disc (402) and the second magnetic disc (403) are magnetically connected.
4. A pepsin enzymolysis tank according to claim 2, wherein: the top fixed mounting of ring seat (201) has frame shell (5) of vertical setting, and the inside top fixed mounting of frame shell (5) has pressure sensor (501), the inside movable mounting of frame shell (5) has floater (502) that are located pressure sensor (501) below, touch switch is installed equally to the bottom of ring seat (201).
5. The pepsin enzymolysis tank according to claim 1, wherein: the heat preservation tank (6) is fixedly installed on the outer end wall of the main tank body (1), and the first back suction pipe (103) is spirally arranged inside the heat preservation tank (6).
6. The pepsin enzymolysis tank of claim 5, wherein: an electric heating module (601) and a temperature control switch (602) are respectively and fixedly arranged in the heat preservation tank (6).
CN202311126788.6A 2023-09-04 2023-09-04 Pepsin enzymolysis tank Active CN116875452B (en)

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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3902620A1 (en) * 1988-02-15 1989-08-17 Hermann Boeck Device for producing fermented berry juice
CN205443317U (en) * 2016-02-22 2016-08-10 福建师范大学 Fountain enzymolysis tank of preparation fish scale collagen oligopeptide
CN206188808U (en) * 2016-07-15 2017-05-24 安徽精准医疗产业研究院有限公司 Pepsin enzymatic hydrolysis device
CN210560471U (en) * 2019-07-08 2020-05-19 拓普河北生物科技有限公司 High-efficient micromolecule peptide powder apparatus for producing
CN211847889U (en) * 2020-01-16 2020-11-03 单县舜天专用车制造有限公司 Fermentation bacterial spray set
CN114231402A (en) * 2021-11-18 2022-03-25 吴宇翔 Pepsin extraction equipment and extraction method
CN116640658A (en) * 2023-04-11 2023-08-25 国药肽谷有限公司 Bovine bone collagen peptide production device and production method thereof

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3902620A1 (en) * 1988-02-15 1989-08-17 Hermann Boeck Device for producing fermented berry juice
CN205443317U (en) * 2016-02-22 2016-08-10 福建师范大学 Fountain enzymolysis tank of preparation fish scale collagen oligopeptide
CN206188808U (en) * 2016-07-15 2017-05-24 安徽精准医疗产业研究院有限公司 Pepsin enzymatic hydrolysis device
CN210560471U (en) * 2019-07-08 2020-05-19 拓普河北生物科技有限公司 High-efficient micromolecule peptide powder apparatus for producing
CN211847889U (en) * 2020-01-16 2020-11-03 单县舜天专用车制造有限公司 Fermentation bacterial spray set
CN114231402A (en) * 2021-11-18 2022-03-25 吴宇翔 Pepsin extraction equipment and extraction method
CN116640658A (en) * 2023-04-11 2023-08-25 国药肽谷有限公司 Bovine bone collagen peptide production device and production method thereof

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