CN114231402A - Pepsin extraction equipment and extraction method - Google Patents
Pepsin extraction equipment and extraction method Download PDFInfo
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- CN114231402A CN114231402A CN202111367835.7A CN202111367835A CN114231402A CN 114231402 A CN114231402 A CN 114231402A CN 202111367835 A CN202111367835 A CN 202111367835A CN 114231402 A CN114231402 A CN 114231402A
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- filter screen
- pepsin
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- 238000000605 extraction Methods 0.000 title claims abstract description 70
- 102000057297 Pepsin A Human genes 0.000 title claims abstract description 59
- 108090000284 Pepsin A Proteins 0.000 title claims abstract description 59
- 229940111202 pepsin Drugs 0.000 title claims abstract description 59
- 239000007788 liquid Substances 0.000 claims abstract description 28
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical group [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 16
- 229910052742 iron Inorganic materials 0.000 claims abstract description 8
- 239000011343 solid material Substances 0.000 claims description 43
- 210000002784 stomach Anatomy 0.000 claims description 42
- 210000004400 mucous membrane Anatomy 0.000 claims description 35
- 238000007789 sealing Methods 0.000 claims description 30
- 239000000463 material Substances 0.000 claims description 27
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 20
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 239000013013 elastic material Substances 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 4
- 229940088598 enzyme Drugs 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 239000002244 precipitate Substances 0.000 claims description 3
- 238000001556 precipitation Methods 0.000 claims description 3
- 238000007873 sieving Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 13
- 239000012528 membrane Substances 0.000 abstract description 2
- 238000009288 screen filtration Methods 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 110
- 210000004877 mucosa Anatomy 0.000 description 28
- 230000009471 action Effects 0.000 description 21
- 210000002599 gastric fundus Anatomy 0.000 description 14
- 239000007790 solid phase Substances 0.000 description 10
- 238000001914 filtration Methods 0.000 description 9
- 230000020477 pH reduction Effects 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 6
- 210000001156 gastric mucosa Anatomy 0.000 description 6
- 230000009286 beneficial effect Effects 0.000 description 5
- 230000000903 blocking effect Effects 0.000 description 5
- 238000001125 extrusion Methods 0.000 description 5
- 230000002349 favourable effect Effects 0.000 description 5
- 230000005484 gravity Effects 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 238000005360 mashing Methods 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 3
- 238000000227 grinding Methods 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 108010047320 Pepsinogen A Proteins 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 230000002496 gastric effect Effects 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 238000010992 reflux Methods 0.000 description 2
- 238000005096 rolling process Methods 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 108010033276 Peptide Fragments Proteins 0.000 description 1
- 102000007079 Peptide Fragments Human genes 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 108091007735 digestive proteases Proteins 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 201000006549 dyspepsia Diseases 0.000 description 1
- 210000004211 gastric acid Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/14—Bioreactors or fermenters specially adapted for specific uses for producing enzymes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/087—Cleaning containers, e.g. tanks by methods involving the use of tools, e.g. brushes, scrapers
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- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6478—Aspartic endopeptidases (3.4.23)
- C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
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Abstract
The invention relates to the technical field of pepsin processing, in particular to pepsin extraction equipment and a pepsin extraction method, wherein the pepsin extraction equipment comprises a motor, a first telescopic rod, a tamping head, an outer shell and an inner shell, wherein the motor is fixed at the top of the outer shell, an output shaft of the motor extends into the inner part of the outer shell and is fixedly connected with the first telescopic rod, and the bottom end of the first telescopic rod is fixedly connected with the tamping head; through setting up the filter screen, the closed loop, second telescopic link and kicking block, the kicking block is iron and the middle part of filter screen lower surface evenly is equipped with magnet, when the second telescopic link is driving the kicking block downstream through the closed loop, the filter screen lower surface will be dragged gradually and become conical, and when the kicking block is greater than the appeal of magnet to the pulling force of filter screen, the kicking block will contact with the filter screen disconnection, and have elastic filter screen and upwards bounce rapidly, thereby clear up the sticky membrane liquid on the filter screen surface automatically, the effect of follow-up filter screen filtration extract has further been improved.
Description
Technical Field
The invention relates to the technical field of pepsin processing, in particular to equipment and a method for extracting pepsin.
Background
Pepsin is a digestive protease secreted from the main cells of the gastric mucosa in the stomach and functions to break down proteins in food into small peptide fragments; the main cell secretes pepsinogen which is formed by gastric acid or pepsin stimulation, the pepsinogen is not directly generated by cells, the pepsin is the first enzyme obtained from animals, and the medicinal pepsin can be extracted from the stomachs of pigs, cows and sheep and is used for dyspepsia.
A patent related to a pepsin extraction device and a pepsin extraction method also appears in the prior art, and for example, a chinese patent with the application number of cn201820107146.x discloses a pepsin rough-making tank, which comprises an acidification cavity and a filtration cavity which are movably connected in a sealing manner. The acidification cavity is internally provided with a cutter disc, a filter screen is fixedly arranged at the position of the filter cavity close to the top opening, and a material opening with a control valve is arranged at the bottom. The acidification cavity is fixedly connected with a rotating shaft of the rotating frame and is controlled by a control power supply to vertically turn in the rotating frame. Above-mentioned utility model changes swing joint's acidizing cavity and the spatial position who filters the cavity through the rotation axis of control power regulation and control to the realization is thrown the quick separation of material and pig stomach end mucous membrane solid material and extract, is favorable to improving production efficiency. Furthermore, the pig gastric fundus mucosa is continuously and synchronously crushed and stirred by the cutter disc arranged in the acidification cavity in the acidification treatment process, so that pepsin is fully separated out, and the acidification extraction quality and efficiency are improved; however, the utility model still has defects, the utility model can only crush and stir the solid material of the pig stomach bottom mucous membrane in the acidification cavity through the cutter disc at the bottom, so that the solid material of the pig stomach bottom mucous membrane is insufficiently reacted with hydrochloric acid and water, thereby slowing down the efficiency of obtaining the pepsin extracting solution, and the residual solid material after stirring can block the filter screen, thereby preventing the extracting solution from being discharged from the material port, and simultaneously, the adhesive membrane liquid on the surface of the filter screen can not be cleaned, so that the use efficiency of the extracting equipment is poor; so that the technical solution is limited.
In view of the above, the present invention provides a pepsin extraction apparatus and a pepsin extraction method, so as to solve the above technical problems.
Disclosure of Invention
In order to make up the defects of the prior art and solve the problems that the reaction of the solid material of the porcine gastric fundus mucosa with hydrochloric acid and water is not sufficient in the prior pepsin extraction process, the filter screen cannot be blocked by the residual solid material, the mucosa liquid on the surface of the filter screen cannot be automatically cleaned, and the like; the invention provides equipment and a method for extracting pepsin.
The technical scheme adopted by the invention for solving the technical problems is as follows: a pepsin extraction device comprises a motor, a first telescopic rod, a tamping head, an outer shell and an inner shell, wherein the upper end of the outer shell is in threaded connection with a top cover, the motor is fixed at the top of the outer shell, an output shaft of the motor extends into the outer shell and is fixedly connected with the first telescopic rod, the bottom end of the first telescopic rod is fixedly connected with the tamping head, the bottom surface of the tamping head is hemispherical, and the upper surface of the tamping head is an arc surface; the inner shell is fixedly connected with the inner surface of the bottom of the outer shell, and the inner surface of the inner shell is hemispherical and is opposite to the bottom of the tamping head; the outer surface of the top of the inner shell is fixedly connected with the side wall of the outer shell; a closed area formed between the outer surface of the bottom of the inner shell and the inner surface of the bottom of the outer shell is an extraction area; a liquid outlet pipe is arranged at the bottom of the extraction area and is obliquely connected with the inner surface of the shell; the upper surface of the top of the extraction area is an inclined plane and is provided with an annular groove, and an annular filter screen is fixedly connected in the annular groove; the bottom of the annular groove is provided with a sealing unit which is used for sealing the bottom of the annular groove;
when the device is used, firstly, the upper end of the outer shell is rotated to separate the top cover at the upper end from the bottom of the outer shell, hydrochloric acid and an aqueous solution are mixed according to a proportion to obtain an extracting solution, the extracting solution is poured into the inner shell, then, a solid material of the mucous membrane at the bottom of the stomach of a pig is placed into the inner shell and is fully mixed with the extracting solution in the inner shell, and then, the sealing unit is started to keep the bottom of the annular groove closed; a user starts a motor on the top surface of the outer shell through an external controller, the motor drives the first telescopic rod and the tamping head to rotate in the outer shell, the pig stomach fundus mucous membrane in the material to be processed is smashed and then is contacted with the extracting solution more fully, then the end part of the first telescopic rod is started to move downwards, the end part of the first telescopic rod drives the tamping head to move downwards and is contacted with the material to be processed in the inner shell, the rotating tamping head enables the material to be processed to be further crushed under the rolling action, and pepsin in the pig stomach fundus mucous membrane is fully separated from the pig stomach fundus mucous membrane and is dissolved in the extracting solution; continuously grinding the extracting solution consisting of the solid material of the mucous membrane of the gastric fundus of the pig, hydrochloric acid and water in the inner shell by the pounding head; the upper surface of the tamping head is a circular arc surface, so that solid materials in the extracting solution are prevented from remaining on the upper surface of the tamping head; the bottom surface of the tamping head is hemispherical and corresponds to the hemisphere of the inner surface of the inner shell, so that the extracting solution in the inner shell is continuously extruded by the tamping head, and the extracting solution slides to the surface of the filter screen along the inner wall of the inner shell; the solid-phase pig stomach fundus mucosa is difficult to move upwards and pass through a small gap between the pounding head and the inner shell due to large volume, so that the solid-phase pig stomach fundus mucosa is limited in the inner shell to be in full contact with the pounding head and processed; the annular groove is kept closed due to the action of the closing unit, so that the phenomenon that the extracting solution which is not dissolved into a large amount of pepsin at the beginning of processing flows out of the annular groove to influence the utilization efficiency of the extracting solution is avoided; after the pounding head moves upwards and resets, because the upper surface of the top of the extracting area is an inclined plane, the extracting solution which is not pressed flows back and enters the inner shell to impact the porcine gastric fundus mucosa in the inner shell, so that the porcine gastric fundus mucosa is impacted and stirred, and the phenomenon that a large amount of porcine gastric fundus mucosa is pressed to be tightly attached to the surface of the inner shell to influence the contact with the extracting solution is avoided; after the extracting solution flows back, the tamping head moves downwards again to act on the materials in the inner shell, and the operation is repeated; after working for a period of time, the pig gastric fundus mucosa is in full contact reaction with the extracting solution, so that pepsin contained in the pig gastric fundus mucosa is fully extracted, the sealing unit is closed at the moment, the annular groove is opened, and the extracting solution overflows the filter screen, passes through the filter screen and the annular groove to enter the extraction area along with the up-and-down movement of the pounding head; in the processing, because the pig stomach fundus mucosa is limited in the gap between the inner shell and the tamping head, the content of the solid-phase pig stomach fundus mucosa in the extracting solution is less, so that the solid-phase pig stomach fundus mucosa attached to the filter screen under the filtering action of the filter screen is less, and the extracting solution which is subjected to accelerated backflow under the inclined surface action impacts the upper surface of the filter screen, so that the permeability of the filter screen is further ensured, the blockage of the filter screen caused by the solid-phase pig stomach fundus mucosa contained in the extracting solution passing through the filter screen is effectively avoided, the passing filtration of the extracting solution is influenced, and the processing efficiency of the whole extracting process is further reduced; and finally, after the reaction is finished, the extracting solution collected to the bottom of the extracting area is led out through a liquid outlet pipe, and the extraction of the pepsin is finished.
Preferably, the upper surface of the top of the extraction area is uniformly provided with stop rods, the stop rods are annularly distributed around the central shaft of the tamping head, and the stop rods are positioned on one side of the filter screen close to the inner shell;
when the extracting device is used, when the extracting solution passes through the gap between the inner shell and the pounding head under the action of the pounding head and flows upwards to the upper surface of the top of the extracting area, the extracting solution firstly passes through the gap between the stop rods due to the action of the stop rods which are distributed annularly, and massive pig stomach fundus mucous membranes in the extracting solution are blocked by the stop rods and cannot contact with the filter screen through the stop rods, so that the blockage of the filter screen caused by the massive pig stomach fundus mucous membranes is further avoided, and the normal play of the filter screen is influenced; when the extracting solution flows back, the massive pig stomach fundus mucous membrane limited in the gap of the stop lever falls back into the inner shell under the impact of the flowing extracting solution, so that the situation that the massive pig stomach fundus mucous membrane is clamped in the gap between the stop levers to influence the normal function of the stop lever is avoided.
Preferably, the end part of the stop lever is bent upwards, the stop lever is made of rigid material, and the joint of the stop lever and the top surface of the inner shell is made of elastic material; the diameter of a circle corresponding to the end part of the stop lever is smaller than that of the outer ring of the tamping head;
when the tamping tool is used, the end part of the bent stop lever is tightly attached to the bottom of the tamping head which slides downwards, so that the tamping head can move downwards conveniently and the tamping head is prevented from being clamped by the stop lever; when the bottom of the tamping head is contacted with the liquid level of the extracting solution in the inner shell, the inner side surfaces of the baffle rods which are distributed in an annular shape are tangent to the semicircular bottom of the tamping head, so that the baffle rods which are distributed uniformly form a semi-closed space with the bottom of the tamping head, and solid materials in the extracting solution are prevented from passing through the baffle rods and sliding to the surface of the filter screen, thereby preventing the solid materials in the extracting solution from blocking the filter holes of the filter screen; the connecting part of the stop lever made of rigid material and the elastic material is beneficial to reducing the action of friction force between the inner side surface of the stop lever and the bottom surface of the tamping head, and is beneficial to the action that the inner side surface of the stop lever is extruded by the bottom of the tamping head to bend towards two sides, so that the phenomenon that the stop lever which is uniformly distributed is extruded by the tamping head to bend towards the central shaft direction due to overlarge friction force between the inner side surface of the stop lever and the bottom surface of the tamping head is prevented; simultaneously through the setting to pin tip height for when the extract liquid level in the inner shell with smash the head bottom contact, the pin tip has been through smashing head bottom surface contact, thereby the extract of guaranteeing to receive the extrusion to move up receives the screening effect of evenly arranging the pin fully.
Preferably, the outer side surface of the tamping head is fixedly connected with an annular fixed block, and the section of the fixed block is trapezoidal;
during the use, smash the head and move annular fixed block and slide downwards, and the big and big one end in fixed block cross-section of fixed block up end cross-section is close to smash the first upper surface, makes the fixed block and the just frictional force of contact of pin reduce, is convenient for the fixed block along the slide of pin inboard surface to make the fixed block carry out the effect of scraping to remaining solid material on the pin inboard surface.
Preferably, a second groove is formed in the fixed block, the second groove penetrates through the fixed block from top to bottom, and the section of the second groove is conical;
when the fixed block is used, when the fixed block scrapes off the residual solid materials on the inner side surface of the stop lever, part of the residual solid materials may stick to the bottom of the fixed block; the upper surface of the sealing unit coincides with the annular groove, and the upper surface of the top of the extraction area is an inclined plane, so that the extracting solution on the surface of the filter screen flows back into the inner shell through the gravity of the extracting solution, and the bottom of the fixed block is washed by the refluxing extracting solution through the second notch, so that the effect of removing the residual solid materials stuck at the bottom of the fixed block is achieved, and the cleaning efficiency of the fixed block is further improved.
Preferably, the outer side surface of the fixed block is inclined and uniformly provided with a first hole, the section of the first hole is circular, and the second groove is communicated with the first hole in the fixed block;
during the use, when the fixed block strikes off the solid material of pin inboard surface residual, probably have partial residual solid material to glue the fixed block outside, simultaneously when the extract of backward flow washes the fixed block bottom through No. two grooves, probably the extract of partial backward flow washes the outside of fixed block slope through a hole to play and detach the residual solid material effect that fixed block outside surface stuck, further improved the clean efficiency to the fixed block.
Preferably, the sealing unit comprises a supporting rod, a second telescopic rod and a sealing ring, the supporting rod is fixedly connected to the inner surface of the shell below the annular groove, and the upper surface of the supporting rod is fixedly connected with the second telescopic rod; the end surface of the second telescopic rod is fixedly connected with an annular closed ring, the supporting rod is inclined to the inner surface of the shell, and the surface of the supporting rod is provided with a first groove;
when the extraction device is used, the sealing unit is opened to enable the second telescopic rod to drive the sealing ring to move upwards, so that the sealing ring keeps closing the annular groove at the top of the extraction area, and the phenomenon that extraction liquid which is not dissolved into a large amount of pepsin at the beginning of processing flows out of the annular groove and the utilization efficiency of the extraction liquid is influenced is avoided; after working for a period of time, after the mucous membrane at the gastric bottom of the pig is in full contact reaction with the extracting solution, the second telescopic rod drives the closed ring to move downwards when the closed unit is closed, so that the closed ring is separated from the annular groove at the top of the extraction area, and along with the up-and-down movement of the tamping head, the extracting solution overflows the filter screen, passes through the filter screen and the annular groove and enters the extraction area; wherein some extract passes through the filter screen landing to the bracing piece on the surface, and this extract slides on the bracing piece surface through self gravity, and through bracing piece on the surface a groove landing to drawing district bottom, draw the bottom in district to set up to the inclined plane to play and improve the efficiency that the extract passes through the drain pipe and gets into next step.
Preferably, the filter screen is made of elastic materials, an annular top block is arranged on the closed ring, and the upper surface of the top block is arc-shaped;
during the use, the controller is controlling the second telescopic link and is driving the closed loop and upwards sliding, makes the closed loop upwards extrude and have elastic filter screen to make the filter screen be half arc through annular kicking block on the closed loop in the ring channel, be favorable to blockking the trend that the extract flows to shell inner wall direction, and the filter screen through arc surface is favorable to accelerating the speed that the extract flows back fast in the inner shell.
Preferably, the top block is made of iron, and magnets are uniformly arranged in the middle of the lower surface of the filter screen;
during the use, the controller is controlling the second telescopic link and is driving the closed loop and is sliding down, make the closed loop drive the kicking block upper surface and keep away from the filter screen lower surface gradually, because be equipped with magnet in the middle of the filter screen lower surface, and the kicking block adopts the iron material, so the filter screen lower surface attracts with the kicking block upper surface mutually, along with the kicking block is the downstream, the filter screen lower surface is pulled gradually and is drawn conically, when the pulling force of kicking block to the filter screen is greater than the appeal of magnet, the kicking block will break away from the contact with the filter screen, and have elastic filter screen and will upwards bounce rapidly, thereby clear up the slime liquid on the filter screen surface, prevent that the phenomenon that the slime liquid from plugging up the filter screen filtration pore from appearing, the effect of the filtration extract of follow-up filter screen has further been improved.
A pepsin extraction method is suitable for the pepsin extraction equipment, and comprises the following steps:
s1: switching on a power supply of the extraction equipment, turning on a switch of the extraction equipment, adding water and hydrochloric acid in advance to prepare an extracting solution, and simultaneously performing heating treatment on the material in the inner shell according to the equipment interface indication operation;
s2: when the temperature in the inner shell is heated to 50 ℃, adding the mucous membrane of the pig stomach, controlling the motor 1 through the controller to drive the first telescopic rod and the tamping head to rotate, and simultaneously driving the tamping head to move up and down by the first telescopic rod so that the tamping head smashes the material in the inner shell;
s3: keeping the temperature inside the inner shell between 45 and 48 ℃, continuously mashing and extruding the material along with the mashing head, enabling the extruded liquid to slide to the filter screen along the inner wall of the inner shell, so that undigested solid materials of the pig stomach mucous membrane are removed by the filter screen, and finally discharging the extracting solution out of the extracting equipment through a liquid outlet pipe for further processing;
s4: placing the extract in a jacketed pan, adding chloroform or diethyl ether after the extract is cooled, stirring, transferring the stirred solution into a precipitation defatting device, standing to remove the precipitate, taking out the clear enzyme solution, concentrating and drying under vacuum, and finally sieving by a sieve to obtain the pepsin powder.
The invention has the following beneficial effects:
1. according to the pepsin extraction equipment and the pepsin extraction method, the first telescopic rod, the tamping head, the stop lever and the inner shell are arranged, when the liquid level of the extracting solution in the inner shell is in contact with the bottom of the tamping head, the end part of the stop lever is already in contact with the surface of the bottom of the tamping head, and the bottom of the tamping head, the inner side of the stop lever and the inner wall of the inner shell form a semi-closed space, so that the extracting solution which moves upwards under the extrusion action is fully subjected to the screening action of uniformly arranging the stop lever, solid materials in the extracting solution stay in the semi-closed space, the phenomenon that the solid materials block filter holes of a filter screen is avoided, and the adverse effect of residual solid materials on pepsin extraction is reduced.
2. According to the pepsin extraction equipment and the pepsin extraction method, the filter screen, the closed ring, the second telescopic rod and the top block are arranged, the top block is made of iron, the magnets are uniformly arranged in the middle of the lower surface of the filter screen, when the second telescopic rod drives the top block to move downwards through the closed ring, the lower surface of the filter screen is gradually pulled to be conical, and when the pulling force of the top block on the filter screen is larger than the attraction force of the magnets, the top block is disconnected with the filter screen, and the elastic filter screen can be rapidly sprung upwards, so that the mucous membrane liquid on the surface of the filter screen is automatically cleaned, and the effect of filtering an extracting solution by the subsequent filter screen is further improved.
Drawings
The invention will be further explained with reference to the drawings.
FIG. 1 is a flow chart of the extraction method of the present invention;
FIG. 2 is a perspective view of the extraction apparatus of the present invention;
FIG. 3 is a cross-sectional view of the extraction apparatus of the present invention;
FIG. 4 is an enlarged view of a portion of FIG. 3 at A;
fig. 5 is a view of the tamper head of fig. 1;
FIG. 6 is a partial enlarged view at B in FIG. 5;
FIG. 7 is an enlarged view of a portion of FIG. 4 at C;
in the figure: 1. a motor; 2. a first telescopic rod; 3. tamping a head; 4. a housing; 41. a support bar; 42. a second telescopic rod; 43. a closed ring; 44. a first groove; 45. a top block; 5. an inner shell; 6. an extraction zone; 61. an annular groove; 62. a filter screen; 63. a stop lever; 7. a fixed block; 71. a second groove; 72. a first hole.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
As shown in fig. 1 to 7, a pepsin extraction device comprises a motor 1, a first telescopic rod 2, a tamping head 3, an outer shell 4 and an inner shell 5, wherein a top cover is connected to the upper end of the outer shell 4 through a thread, the motor 1 is fixed on the upper surface of the top cover on the outer shell 4, an output shaft of the motor 1 extends into the inner shell 4 and is fixedly connected with the first telescopic rod 2, the tamping head 3 is fixedly connected to the bottom end of the first telescopic rod 2, the bottom surface of the tamping head 3 is hemispherical, and the upper surface of the tamping head 3 is an arc surface; the inner shell 5 is fixedly connected with the inner surface of the bottom of the outer shell 4, and the inner surface of the inner shell 5 is hemispherical and is opposite to the bottom of the tamping head 3; the outer surface of the top of the inner shell 5 is fixedly connected with the side wall of the outer shell 4; the closed area formed between the outer surface of the bottom of the inner shell 5 and the inner surface of the bottom of the outer shell 4 is an extraction area 6; a liquid outlet pipe is arranged at the bottom of the extraction area 6 and is obliquely connected with the inner surface of the shell 4; the upper surface of the top of the extraction area 6 is an inclined plane and is provided with an annular groove 61, and an annular filter screen 62 is fixedly connected in the annular groove 61; the bottom of the annular groove 61 is provided with a sealing unit which is used for sealing the bottom of the annular groove 61;
when the device is used, firstly, the upper end of the outer shell 4 is rotated to separate the top cover at the upper end from the bottom of the outer shell 4, hydrochloric acid and water solution are mixed according to a proportion to obtain extracting solution, the extracting solution is poured into the inner shell 5, then, the solid material of the mucous membrane at the gastric fundus of the pig is placed into the inner shell 5 and is fully mixed with the extracting solution in the inner shell, and then, the sealing unit is started to keep the bottom of the annular groove 61 closed; a user starts the motor 1 on the top surface of the outer shell 4 through an external controller, so that the motor 1 drives the first telescopic rod 2 and the tamping head 3 to rotate in the outer shell 4, the pig stomach fundus mucous membrane in the material to be processed is smashed and then is contacted with the extracting solution more fully, then the end part of the first telescopic rod 2 is started to move downwards, so that the end part of the first telescopic rod 2 drives the tamping head 3 to move downwards and is contacted with the material to be processed in the inner shell 5, the rotating tamping head 3 enables the material to be processed to be further crushed by rolling action, and pepsin in the pig stomach fundus mucous membrane is fully separated from the pig stomach fundus mucous membrane and dissolved in the extracting solution; continuously grinding the extracting solution consisting of the solid material of the mucous membrane of the stomach bottom of the pig, the hydrochloric acid and the water in the inner shell 5 by the pounding head 3; the upper surface of the tamping head 3 is a circular arc surface, so that solid materials in the extracting solution are prevented from remaining on the upper surface of the tamping head 3; the bottom surface of the tamping head 3 is hemispherical and corresponds to the hemisphere of the inner surface of the inner shell 5, so that the extracting solution in the inner shell 5 is continuously extruded by the tamping head 3, and the extracting solution slides to the surface of the filter screen 62 along the inner wall of the inner shell 5; the solid-phase pig stomach fundus mucosa is difficult to move upwards and pass through a small gap between the pounding head 3 and the inner shell 5 because of large volume, so that the solid-phase pig stomach fundus mucosa is limited in the inner shell 5 to be fully contacted with the pounding head 3 and processed; the annular groove 61 is kept closed due to the action of the closing unit, so that the phenomenon that the extracting solution which is not dissolved into a large amount of pepsin at the beginning of processing flows out of the annular groove 61 to influence the utilization efficiency of the extracting solution is avoided; after the pounding head 3 moves upwards and resets, because the upper surface of the top of the extracting area 6 is an inclined surface, the extracting solution which is not pressed flows back and enters the inner shell 5 to impact the porcine gastric mucosa in the inner shell 5, so that the porcine gastric mucosa is impacted and stirred, and the phenomenon that a large amount of porcine gastric mucosa is pressed to be tightly attached to the surface of the inner shell 5 to influence the contact with the extracting solution is avoided; after the extracting solution flows back, the tamping head 3 moves downwards again to act on the materials in the inner shell 5, and the process is repeated; after working for a period of time, the pig gastric fundus mucosa is in full contact reaction with the extracting solution, so that pepsin contained in the pig gastric fundus mucosa is fully extracted, the sealing unit is closed at the moment, the annular groove 61 is opened, and the extracting solution overflowing the filter screen 62 passes through the filter screen 62 and the annular groove 61 to enter the extracting area 6 along with the up-and-down movement of the tamping head 3; in the processing, because the pig stomach fundus mucosa is limited in the gap between the inner shell 5 and the tamping head 3, the content of the solid-phase pig stomach fundus mucosa in the extracting solution is less, so that the solid-phase pig stomach fundus mucosa attached to the filter screen 62 under the filtering action of the filter screen 62 is less, and the extracting solution which is subjected to accelerated backflow under the inclined surface action impacts the upper surface of the filter screen 62, so that the permeability of the filter screen 62 is further ensured, the blockage of the filter screen 62 caused by the solid-phase pig stomach fundus mucosa contained in the extracting solution passing through the filter screen 62 is effectively avoided, the passing filtration of the extracting solution is influenced, and the processing efficiency of the whole extracting process is further reduced; and finally, after the reaction is finished, the extracting solution collected to the bottom of the extracting area 6 is led out through a liquid outlet pipe, and the extraction of the pepsin is finished.
As a specific embodiment of the present invention, the upper surface of the top of the extraction area 6 is uniformly provided with stop rods 63, the stop rods 63 are annularly distributed around the central axis of the tamper head 3, and the stop rods 63 are positioned on one side of the filter screen 62 close to the inner shell 5;
when the extracting solution is used, when the extracting solution passes through the gap between the inner shell 5 and the pounding head 3 under the action of the pounding head 3 and flows upwards to the upper surface of the top of the extracting area 6, the extracting solution firstly passes through the gap between the stop rods 63 due to the action of the stop rods 63 which are distributed annularly, and massive pig gastric fundus mucous membranes with large volumes in the extracting solution are stopped by the stop rods 63 and cannot contact with a filter screen through the stop rods 63, so that the blockage of the filter screen caused by the massive pig gastric fundus mucous membranes is further avoided, and the normal play of the filter screen 62 is influenced; when the extracting solution flows back, the massive pig stomach fundus mucous membrane limited in the gap of the stop rod 63 falls back into the inner shell 5 under the impact of the flowing extracting solution, so that the massive pig stomach fundus mucous membrane is prevented from being clamped in the gap between the stop rods 63 to influence the normal function of the stop rod 63.
As a specific embodiment of the present invention, the end of the stop lever 63 is bent upward, the stop lever 63 is made of rigid material, and the joint between the stop lever 63 and the top surface of the inner shell 5 is made of elastic material; the diameter of a circle corresponding to the end part of the stop lever 63 is smaller than the diameter of the outer ring of the tamping head 3;
when in use, the end part of the bent stop rod 63 is tightly attached to the bottom of the tamping head 3 which slides downwards, so that the tamping head 3 can move downwards conveniently and the tamping head 3 is prevented from being clamped by the stop rod 63; when the bottom of the tamping head 3 is contacted with the liquid level of the extracting solution in the inner shell 5, the inner side surface of the blocking rods 63 which are distributed in an annular shape is tangent to the semicircular bottom of the tamping head 3, so that the blocking rods 63 which are distributed uniformly form a semi-closed space with the bottom of the tamping head 3, solid materials in the extracting solution are prevented from passing through the blocking rods 63 and sliding to the surface of the filter screen 62, and the phenomenon that the solid materials in the extracting solution block the filter holes of the filter screen 62 is prevented; the connecting part of the stop rod 63 made of rigid material and the elastic material is beneficial to reducing the action of friction force between the inner side surface of the stop rod 63 and the bottom surface of the tamping head 3, and is beneficial to the action that the inner side surface of the stop rod 63 is extruded by the bottom of the tamping head 3 to bend towards two sides, so that the phenomenon that the stop rod 63 which is uniformly distributed is extruded by the tamping head 3 to bend towards the central shaft direction due to overlarge friction force between the inner side surface of the stop rod 63 and the bottom surface of the tamping head 3 is prevented; simultaneously through the setting to pin 63 tip height for when the extract liquid level in the inner shell 5 with smash the contact of head 3 bottom, pin 63 tip has with smash the contact of head 3 bottom surface, thereby guarantees that the extract that receives the extrusion to move up fully receives the screening effect of evenly arranging pin 63, has further guaranteed the efficiency that filter screen 62 filtered the extract.
As a specific embodiment of the invention, the outer side surface of the tamping head 3 is fixedly connected with an annular fixed block 7, and the section of the fixed block 7 is trapezoidal;
during the use, it is downward that head 3 is being driven annular fixed block 7, and the big and big one end in fixed block 7 cross-section of fixed block 7 up end cross-section is close to and smashes first 3 upper surfaces, thereby make fixed block 7 and pin 63 contact surface more smooth, be convenient for fixed block 7 along pin 63 inboard side surface slide, make fixed block 7 carry out the effect of striking off to remaining solid material on 63 side surface in the pin, avoid remaining solid material card clearance influence extract to pass through pin 63 and enter into ring channel 61 between the pin 63, and then influenced the efficiency that the extract passes through ring channel 61.
As a specific embodiment of the present invention, a second groove 71 is formed in the fixing block 7, the second groove 71 penetrates through the fixing block 7 from top to bottom, and an inner surface of the second groove 71 is tapered;
when the scraper is used, when the fixed block 7 scrapes off the solid materials remained on the inner side surface of the stop rod 63, part of the remained solid materials may stick to the bottom of the fixed block 7; simultaneously, the upper surface of the sealing unit coincides with the annular groove 61, and the upper surface of the top of the extraction area 6 is an inclined plane, so that the extracting solution on the surface of the filter screen 62 flows into the inner shell 5 again through the gravity of the extracting solution, and part of the refluxing extracting solution washes the bottom of the fixing block 7 through the second notch 71, so that the residual solid material adhered to the bottom of the fixing block 7 is removed, and the cleaning efficiency of the fixing block 7 is further improved.
As a specific embodiment of the present invention, the outer surface of the fixing block 7 is inclined and uniformly provided with a first hole 72, the section of the first hole 72 is circular, and the second groove 71 and the first hole 72 are communicated inside the fixing block 7;
during the use, when fixed block 7 strikes off the solid material of pin 63 inboard surface residual, probably have partial residual solid material to glue the fixed block 7 outside, simultaneously when the extract of backward flow washes the fixed block bottom through No. two grooves 71, probably the extract of partial backward flow washes the outside that fixed block 7 inclines through a hole 72, thereby play and detach the residual solid material effect that fixed block 7 outside surface stuck, further improved the clean efficiency to fixed block 7, guaranteed that fixed block 7 can normally function.
As a specific embodiment of the present invention, the closing unit includes a supporting rod 41, a second telescopic rod 42 and a closing ring 43, the supporting rod 41 is fixedly connected to the inner surface of the housing 4 below the annular groove 61, and the upper surface of the supporting rod 41 is fixedly connected to the second telescopic rod 42; the end surface of the second telescopic rod 42 is fixedly connected with an annular closed ring 43, the support rod 41 is inclined to the inner surface of the shell, and the surface of the support rod 41 is provided with a first groove 44;
when the extraction device is used, the sealing unit is opened to enable the second telescopic rod 42 to drive the sealing ring 43 to move upwards, so that the sealing ring 43 keeps closing the annular groove 61 at the top of the extraction area 6, and the phenomenon that the extracting solution which is not dissolved into a large amount of pepsin at the beginning of processing flows out of the annular groove 61 is avoided, and the utilization efficiency of the extracting solution is influenced; after working for a period of time, after the mucous membrane at the gastric bottom of the pig is fully contacted and reacted with the extracting solution, when the sealing unit is closed, the second telescopic rod 42 drives the sealing ring 43 to move downwards, so that the sealing ring 43 is separated from the annular groove 61 at the top of the extracting area 6, and the extracting solution passing through the filter screen 62 enters the extracting area 6 through the filter screen 62 and the annular groove 61 along with the up-and-down movement of the tamping head 3; wherein a part of extract passes through filter screen 62 landing to bracing piece 41 on the surface, and this extract slides on bracing piece 41 surface through self gravity, and through bracing piece 41 on the surface a groove 44 landing to draw the district 6 bottom, draw the bottom of district 6 and set up to the inclined plane to play and improve the efficiency that the extract passes through the drain pipe and gets into next step.
As a specific embodiment of the present invention, the filter screen 62 is made of an elastic material, the closed ring 43 is provided with an annular top block 45, and the upper surface of the top block 45 is arc-shaped;
during the use, the controller is controlling second telescopic link 42 and is driving closed ring 43 upwards to slide, makes closed ring 43 upwards extrude to have elastic filter screen 62 to it is half arc to make filter screen 62 be in annular groove 61 through annular kicking block on closed ring 43, is favorable to blockking the trend that the extract flows to shell 4 inner wall direction, and is favorable to accelerating the speed that the extract flows back to in the inner shell 5 fast through the filter screen 62 on arc surface.
As a specific embodiment of the present invention, the top block 45 is made of iron, and the middle portion of the lower surface of the filter screen 62 is uniformly provided with magnets;
during the use, the controller is controlling second telescopic link 42 and is driving closed ring 43 and is sliding downwards, make closed ring 43 drive kicking block 45 upper surface and keep away from filter screen 62 lower surface gradually, because be equipped with magnet in the middle of the filter screen 62 lower surface, and kicking block 45 adopts the iron material, so filter screen 62 lower surface and kicking block 45 upper surface attract mutually, along with kicking block 45 moves downwards, filter screen 62 lower surface is dragged the toper gradually, when kicking block 45 is greater than the appeal of magnet to the pulling force of filter screen 62, kicking block 45 will break off the contact with filter screen 62, and have elastic filter screen 62 and upwards bounce rapidly, thereby clear up the surperficial mucous membrane liquid of filter screen 62, prevent that the phenomenon that mucous membrane liquid blocks up filter screen 62 filtration pore from appearing, further improved the effect of the filter extract of follow-up filter screen 62.
A pepsin extraction method is suitable for the pepsin extraction equipment, and comprises the following steps:
s1: switching on the power supply of the extraction equipment, turning on the switch of the extraction equipment, adding water and hydrochloric acid in advance to prepare an extracting solution in the inner shell 5, and simultaneously performing heating treatment on the material in the inner shell 5 according to the indication operation of an equipment interface;
s2: when the temperature inside the inner shell 5 is heated to 50 ℃, adding the mucous membrane of the pig stomach, controlling the motor 1 through the controller to drive the first telescopic rod 2 and the tamping head 3 to rotate, and simultaneously driving the tamping head 3 to move up and down by the first telescopic rod 2 so that the tamping head 3 smashes the material inside the inner shell 5;
s3: keeping the temperature inside the inner shell 5 between 45 and 48 ℃, continuously mashing and extruding the material along with the mashing head, and enabling the extruded liquid to slide to the filter screen 62 along the inner wall of the inner shell 5, so that undigested solid materials of the pig stomach mucous membrane are removed by the filter screen 62, and finally, the extracting solution is discharged out of the extracting equipment through the liquid outlet pipe for further processing;
s4: placing the extract in a jacketed pan, adding chloroform or diethyl ether after the extract is cooled, stirring, transferring the stirred solution into a precipitation defatting device, standing to remove the precipitate, taking out the clear enzyme solution, concentrating and drying under vacuum, and finally sieving by a sieve to obtain the pepsin powder.
The specific working process is as follows:
firstly, separating the upper part and the bottom of an outer shell 4 by rotating the upper end of the outer shell 4, adding a proper amount of solid material of the porcine gastric mucosa into an inner shell 5 filled with hydrochloric acid and water, closing the upper part and the bottom of the outer shell 4, closely contacting the upper part and the bottom of the outer shell with the bottom by rotating, starting an extraction equipment switch, controlling a motor 1 to be started by a controller, driving a first telescopic rod 2 and a tamping head 3 to rotate in the outer shell 4 by the motor 1, and driving the tamping head 3 to move up and down by the first telescopic rod 2; firstly, the sealing unit is opened, the second telescopic rod 42 is controlled by the controller to drive the sealing ring 43 to move upwards, so that the sealing ring 43 is coincided with the annular groove 61, the sealing ring 43 upwards extrudes the filter screen 62 to form a semi-arc shape through the top block 45 on the surface, and the phenomenon that a large amount of extracting solution which is not dissolved in pepsin at the beginning of processing flows out of the annular groove 61 is avoided, and the utilization efficiency of the extracting solution is influenced; meanwhile, when the liquid level of the extracting solution in the inner shell 5 is contacted with the bottom of the tamping head 3, the end part of the stop rod 63 is already contacted with the surface of the bottom of the tamping head 3, so that the stop rod 63, the bottom of the tamping head 3 and the inner wall of the inner shell 5 form a semi-closed space, thereby the extracting solution moved upwards under the extrusion action is fully screened by the stop rods 63 which are evenly distributed, the solid material of the pig gastric fundus mucosa in the extracting solution is blocked in a semi-closed space by the stop rods 63, therefore, the extracting solution is fully ground in the semi-closed space along with the up-and-down movement of the pounding head 3, and prevents the phenomenon that the filter screen 62 is blocked by the residual solid materials of the pig gastric mucosa, the solution in the extracting solution passes through the stop lever 63 and slides to the surface of the filter screen 62, the top of the extracting area 6 is an inclined plane, and the filter screen 62 is in a semi-arc shape, therefore, the solution flows back into the inner shell 5 in an accelerating way along the surface of the filter screen 62 under the action of self gravity for grinding; meanwhile, when the tamping head 3 moves downwards, the tamping head 3 scrapes off the residual solid materials of the pig stomach bottom mucosa on the inner side of the stop lever 63 through the fixed block 7 on the surface, one part of the residual solid materials of the pig stomach bottom mucosa slide down into the inner shell 5 along with the fixed block 7, and the other part of the residual solid materials of the pig stomach bottom mucosa can be adhered to the bottom and the outer inclined plane of the fixed block 7, so that the backflow solution can respectively wash the bottom and the outer inclined plane of the fixed block through the second groove 71 and the first hole 72, and the cleaning efficiency of the fixed lever 7 is further improved; after working for a period of time, the pig stomach fundus mucosa fully contacts and reacts with the extracting solution, so that pepsin contained in the pig stomach fundus mucosa is fully extracted, the closing unit is closed at the moment, the controller controls the second telescopic rod 42 to drive the closing ring 43 to move downwards, the closing ring 43 is far away from the annular groove 61, along with the up-and-down movement of the pounding head 3, the extracting solution overflowing the filter screen 62 enters the extracting area 6 through the filter screen 62 and passes through the annular groove 61, and a part of the filtered extracting solution slides to the bottom of the extracting area 6 along the groove 44 on the surface of the inclined support rod 41, and finally the extracting solution is transported to the next step along the bottom of the inclined extracting area 6 through the liquid outlet pipe.
Secondly, when the extracting solution moved upwards under the action of extrusion passes through the filter screen 62, the extracting solution may leave a mucous membrane solution on the surface of the filter screen 62, and the mucous membrane solution may block the filter holes on the surface of the filter screen 62, thereby possibly affecting the efficiency of subsequent filtering of the extracting solution, therefore, the extracting device intermittently controls the second telescopic rod 42 to drive the closed ring 43 to slide downwards through the controller, the surface of the closed ring 43 is provided with the ejector block 45, the ejector block 45 is iron, and the middle part of the lower surface of the filter screen 62 is uniformly provided with the magnet, therefore, when the closed ring 43 drives the ejector block 45 to move downwards, the ejector block 45 drives the lower surface of the filter screen 62 to gradually form a cone shape through the magnet, when the pulling force of the ejector block 45 on the lower surface of the filter screen 62 is greater than the attraction force of the magnet, the ejector block 45 is separated from the filter screen 62, and the upper surface of the filter screen 62 with elasticity is sprung upwards, thereby playing a role of preventing the mucous membrane solution from blocking the filter holes of the filter screen 62, further improving the efficiency of extracting the pepsin by the extracting device.
The front, the back, the left, the right, the upper and the lower are all based on the figure 2 in the attached drawings of the specification, according to the standard of the observation angle of a person, the side of the device facing an observer is defined as the front, the left side of the observer is defined as the left, and the like.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate orientations or positional relationships based on those shown in the drawings, and are used merely for convenience in describing the present invention and for simplifying the description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the scope of the present invention.
Finally, it should be pointed out that: the above examples are only for illustrating the technical solutions of the present invention, and are not limited thereto. Although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention. All other embodiments obtained by a person skilled in the art without making any inventive step are within the scope of protection of the present invention.
Claims (10)
1. A pepsin extraction device, which is characterized in that: the tamping tool comprises a motor (1), a first telescopic rod (2), a tamping head (3), an outer shell (4) and an inner shell (5), wherein the upper end of the outer shell (4) is in threaded connection with a top cover, and the motor (1) is fixed at the top of the outer shell (4);
an output shaft of the motor (1) extends into the shell and is fixedly connected with a first telescopic rod (2), a tamping head (3) is fixedly connected to the bottom end of the first telescopic rod (2), the bottom surface of the tamping head (3) is hemispherical, and the upper surface of the tamping head (3) is an arc surface;
the inner shell (5) is fixedly connected with the inner surface of the bottom of the outer shell (4), and the inner surface of the inner shell (5) is hemispherical and is opposite to the bottom of the tamping head (3); the outer surface of the top of the inner shell (5) is fixedly connected with the side wall of the outer shell (4);
a closed area formed between the outer surface of the bottom of the inner shell (5) and the inner surface of the bottom of the outer shell (4) is an extraction area (6); a liquid outlet pipe is arranged at the bottom of the extraction area (6) and is obliquely connected with the inner surface of the shell (4);
the upper surface of the top of the extraction area (6) is an inclined plane and is provided with an annular groove (61), and an annular filter screen (62) is fixedly connected in the annular groove (61);
and a sealing unit is arranged at the bottom of the annular groove (61) and used for sealing the bottom of the annular groove (61).
2. The pepsin extraction apparatus according to claim 1, characterized in that: draw district (6) top upper surface and evenly be equipped with pin (63), pin (63) are around smashing head (3) center pin annular distribution, and pin (63) are located the one side that filter screen (62) are close to inner shell (5).
3. The pepsin extraction apparatus according to claim 2, characterized in that: the utility model discloses a smash head, including fender rod (63), fender rod (63) tip kickup, fender rod (63) adopt rigid material, just fender rod (63) with inner shell (5) top surface junction adopts elastic material, the diameter of the circle that fender rod (63) tip corresponds is less than the diameter of smashing head (3) outer lane.
4. The pepsin extraction apparatus according to claim 2, characterized in that: the outer side surface of the tamping head (3) is fixedly connected with an annular fixing block (7), and the cross section of the fixing block (7) is trapezoidal.
5. The pepsin extraction apparatus according to claim 4, characterized in that: no. two grooves (71) are formed in the fixing block (7), the fixing block (7) penetrates through the groove (71) from top to bottom, and the inner surface of the groove (71) is conical.
6. The pepsin extraction apparatus according to claim 5, characterized in that: the outer side surface of the fixing block (7) is inclined and is uniformly provided with a first hole (72), the section of the first hole (72) is circular, and the second groove (71) is communicated with the first hole (72) in the fixing block (7).
7. The pepsin extraction apparatus according to claim 1, characterized in that: the sealing unit comprises a supporting rod (41), a second telescopic rod (42) and a sealing ring (43), the supporting rod (41) is fixedly connected to the inner surface of the shell (4) below the annular groove (61), and the upper surface of the supporting rod (41) is fixedly connected with the second telescopic rod (42); the end surface of the second telescopic rod (42) is fixedly connected with an annular closed ring (43), the supporting rod (41) is inclined to the inner surface of the shell, and a first groove (44) is formed in the surface of the supporting rod (41).
8. The pepsin extraction apparatus according to claim 7, characterized in that: the filter screen (62) is made of elastic materials, an annular top block (45) is arranged on the closed ring (43), and the upper surface of the top block (45) is arc-shaped.
9. The pepsin extraction apparatus according to claim 8, characterized in that: the top block (45) is made of iron, and magnets are uniformly arranged in the middle of the lower surface of the filter screen (62).
10. A method for extracting pepsin is characterized in that: the extraction method is applied to the pepsin extraction equipment of any one of claims 1-9, and comprises the following steps:
s1: switching on a power supply of the extraction equipment, turning on a switch of the extraction equipment, adding water and hydrochloric acid in advance in the inner shell (5) to prepare an extracting solution, and simultaneously performing heating treatment on the material in the inner shell (5) according to the indication operation of an equipment interface;
s2: when the temperature in the inner shell (5) is heated to 50 ℃, adding the mucous membrane of the pig stomach, controlling the motor (1) to drive the first telescopic rod (2) and the smashing head (3) to rotate through the controller, and simultaneously driving the smashing head (3) to move up and down through the first telescopic rod (2), so that the smashing head (3) smashes materials in the inner shell (5);
s3: the temperature in the inner shell (5) is kept between 45 ℃ and 48 ℃, the material is continuously smashed and extruded along with the smashing head, the extruded liquid slides to the filter screen (62) along the inner wall of the inner shell (5), so that the filter screen (62) removes undigested pig stomach mucous membrane solid materials, and finally the extracting solution is discharged out of the extracting equipment through a liquid outlet pipe for further processing;
s4: placing the extract in a jacketed pan, adding chloroform or diethyl ether after the extract is cooled, stirring, transferring the stirred solution into a precipitation defatting device, standing to remove the precipitate, taking out the clear enzyme solution, concentrating and drying under vacuum, and finally sieving by a sieve to obtain the pepsin powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111367835.7A CN114231402A (en) | 2021-11-18 | 2021-11-18 | Pepsin extraction equipment and extraction method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111367835.7A CN114231402A (en) | 2021-11-18 | 2021-11-18 | Pepsin extraction equipment and extraction method |
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| Publication Number | Publication Date |
|---|---|
| CN114231402A true CN114231402A (en) | 2022-03-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111367835.7A Pending CN114231402A (en) | 2021-11-18 | 2021-11-18 | Pepsin extraction equipment and extraction method |
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| CN (1) | CN114231402A (en) |
Cited By (2)
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| CN114956394A (en) * | 2022-08-02 | 2022-08-30 | 启东胜科水务有限公司 | Sewage treatment device containing mercury metal |
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