CN116875304A - Application of water-soluble sulfonic graphene quantum dot yield increasing agent in promotion of leaf crop yield - Google Patents
Application of water-soluble sulfonic graphene quantum dot yield increasing agent in promotion of leaf crop yield Download PDFInfo
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- 229910021389 graphene Inorganic materials 0.000 title claims abstract description 48
- 230000001965 increasing effect Effects 0.000 title claims abstract description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 52
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims abstract description 42
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 36
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 33
- 230000012010 growth Effects 0.000 claims abstract description 32
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 claims abstract description 28
- 229960003080 taurine Drugs 0.000 claims abstract description 14
- 230000008635 plant growth Effects 0.000 claims abstract description 6
- 235000003228 Lactuca sativa Nutrition 0.000 claims description 60
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 24
- 239000000203 mixture Substances 0.000 claims description 24
- 239000008367 deionised water Substances 0.000 claims description 22
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- 238000005507 spraying Methods 0.000 claims description 17
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/24—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients to enhance the sticking of the active ingredients
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- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
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Abstract
The invention discloses an application of a water-soluble sulfonic graphene quantum dot yield increasing agent in promoting leaf crop yield, and relates to the field of chemical and agricultural technology cross application. According to the invention, taurine and citric acid are adopted to carry out one-step pyrolysis to form sulfonic graphene quantum dots, so that the sunlight absorption ratio of leaf plants is improved, and the photosynthesis of a growth agent is enhanced; the introduction of the sulfonic group enables the quantum dot to have hydrophilicity, can be prepared into a yield-increasing agent with aqueous solution, can be rapidly spread on the surface of a leaf to form a film, effectively prevents water in plants from evaporating, and promotes plant growth.
Description
Technical Field
The invention relates to the field of chemical and agricultural technology cross application, in particular to application of a water-soluble sulfonic graphene quantum dot yield increasing agent in promoting leaf crop yield.
Background
The nano technology has wide development potential and application prospect in the agricultural field. It is widely used in disinfection, photosynthesis promotion, growth hormone regulation and other fields, and provides new possibility for agricultural production. However, the conventional agricultural production methods have failed to meet the demand for grain yield, and thus new production methods and techniques for improving crop yield have been eagerly sought. In the agricultural field, nanotechnology has been applied to regulate photosynthesis to increase crop growth and yield, and one method of applying nanotechnology is to utilize iron metal bioactive nanoparticles that can significantly increase the yield of certain cereal crops by up to 10% to 40%.
Photosynthesis, which is a fundamental process of plant growth and development, directly affects the growth and yield of crops, and thus, improving photosynthesis efficiency has become one of key methods for improving crop yield. Compared with the traditional semiconductor quantum dot, the graphene quantum dot has the obvious advantages of low toxicity, easiness in preparation, surface functionalization, fluorescence stability and the like, and the characteristics enable the graphene quantum dot to be a hot spot research object in the field of novel materials, and is widely focused in recent years and considered to be an effective photosynthesis regulator.
However, the traditional graphene quantum dot material is poor in water solubility due to the fact that the surface of the traditional graphene quantum dot material lacks functional group modification, and is difficult to achieve uniform dispersion when the traditional graphene quantum dot material is prepared into a preparation, so that the inventor carries out modification of sulfonic acid groups on the surface of the graphene quantum dot to greatly improve the dispersibility of the graphene quantum dot material in aqueous solution. In addition, the preparation of the aqueous preparation is environment-friendly, and the preparation can be used more conveniently, and the yield increasing effect is more stable and efficient.
Disclosure of Invention
The invention aims to provide an application of a water-soluble sulfonic graphene quantum dot yield increasing agent in promoting the yield of leaf crops, so as to solve the problems in the background art.
In order to solve the technical problems, the invention provides the following technical scheme: a water-soluble sulfonic graphene quantum dot yield-increasing agent is prepared by dissolving a proper amount of graphene quantum dots in deionized water and regulating the concentration.
Further, the graphene quantum dots are sulfonic group modified graphene quantum dots.
Further, the preparation method of the graphene quantum dot comprises the following steps: mixing citric acid, taurine and secondary distilled water according to the mass ratio of 1:0.7:5-1:0.8:5, placing the mixture into a container, stirring and dissolving the mixture, drying the mixture at 60 ℃ until no moisture exists in the container, transferring the container into a high-temperature oven, preserving heat for 10-12 hours at 180-200 ℃, and cooling the container to room temperature to obtain an intermediate product; mixing the intermediate product and deionized water according to the mass ratio of 1:10, adding sodium hydroxide solution until the pH value of the solution is 7, passing through a 0.22 mu m microporous filter membrane, dialyzing in ultrapure water for 24 hours by using a dialysis bag with the molecular weight cut-off of 3kDa, and freezing at-20 ℃ for 24 hours.
Further, the size of the graphene quantum dot is 2 nm-20 nm.
Further, the concentration of the graphene quantum dots in the yield increasing agent is 0 mg/L-4800 mg/L.
Further, the yield increasing agent is directly applied to the plant.
Further, the application mode is that the leaf surface is sprayed to cover the whole leaf surface and does not gather into water drops, and the spraying time is the plant growth period.
Further, the plants include, but are not limited to, crops with leaves during growth of lettuce, cucumber, corn, and the like.
Compared with the prior art, the invention has the following beneficial effects:
the invention adoptsOne-step pyrolysis of taurine and citric acid to form sulfonic graphene quantum dots, and subsequent carbonization of citric acid to form a graphene quantum dot with sp 2 The graphene sheet formed by carbon atoms, wherein amino groups in taurine molecules are condensed with carboxyl groups at the edges of the graphene sheet to prepare sulfonic graphene quantum dots, the taurine is high in thermal stability, stable molecular structures can be kept at pyrolysis temperature to ensure that functional groups are not decomposed, synthesis of the sulfonic graphene quantum dots is promoted, due to introduction of sulfonic groups, absorption wave bands of the yield increasing agent on sunlight are widened, fluorescence conversion performance of the quantum dots is utilized, so that the absorption ratio of leaf plants on sunlight is improved, and photosynthesis of the growth increasing agent is enhanced.
The sulfonic acid group is introduced into the surface of the quantum dot to promote the water solubility of the quantum dot, so that the quantum dot is easier to apply in aqueous solution, the absorption and the utilization of plants are promoted, thereby realizing the effect of regulating photosynthesis and plant growth more efficiently, simultaneously, the quantum dot has better wettability, better permeability and high pore permeability, and has good rain erosion resistance, in the spraying process, the sulfonic acid group can enhance the surface adhesive force and the spreading force of the graphene quantum dot on the blade, so that the yield-increasing agent is rapidly spread on the surface of the blade to form a film, and the hydrophilicity of the sulfonic acid group is added, so that the quantum dot has better water absorption and water retention capacity, can effectively prevent water evaporation, shows excellent water retention capacity and indirectly improves the growth of the blade plant.
The sulfonic graphene quantum dots are environment-friendly, biodegradable, reduce potential harm to environment and organisms, excite physiological characteristics of leaf plants, induce related enzyme systems, enhance activity of the leaf plants, enable growth points to grow vigorously, promote nutrient absorption of crops, increase accumulation of organic matters, enhance photosynthesis and respiration, increase product yield and improve quality of crops.
The yield increasing agent is relatively simple and easy to prepare, and the spraying application method is simple and efficient, so that the loss of manpower and material resources caused by a dipping mode is reduced.
Drawings
The accompanying drawings are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate the invention and together with the embodiments of the invention, serve to explain the invention. In the drawings:
FIG. 1 is a photograph of graphene quantum dots prepared according to the present invention;
FIG. 2 is a transmission electron microscope image of graphene quantum dots prepared according to the present invention;
FIG. 3 shows the growth of different treated lettuce;
FIG. 4 is a graph of photosynthetic parameters of different treated lettuce.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
Application of graphene quantum dots as yield increasing agents in the aspect of regulating lettuce growth:
(1) Mixing citric acid, taurine and secondary distilled water according to the mass ratio of 1:0.7:5, placing the mixture into a container, stirring and dissolving the mixture, drying the mixture at 60 ℃ until no water exists in the container, transferring the container into a high-temperature oven, preserving heat for 10 hours at 180 ℃, and cooling the container to room temperature to obtain an intermediate product; mixing the intermediate product and deionized water according to the mass ratio of 1:10, adding sodium hydroxide solution until the pH value of the solution is 7, passing through a 0.22 mu m microporous filter membrane, dialyzing in ultrapure water for 24 hours by using a dialysis bag with the molecular weight cutoff of 3kDa, and freezing at-20 ℃ for 24 hours to obtain graphene quantum dots;
(2) Preparing graphene quantum dots and deionized water into graphene quantum dot suspension with concentration of 3600 mg/L;
(3) After lettuce grows to the stage of four leaves and one heart, selecting uniform seedling, transplanting the seedling into a cultivation groove filled with nutrient solution for internal planting, spraying lettuce leaves every five days, washing fresh lettuce leaves with deionized water, and drying the moisture on the surface with absorbent paper; uniformly spraying graphene quantum dot suspension onto lettuce leaves, covering the whole leaf surface, enabling the graphene quantum dot suspension not to be aggregated into water drops to flow down, and naturally airing in the air; harvesting after planting for 21 days, measuring lettuce growth and physiological indexes, and carrying out statistical analysis.
Example 2
Application of graphene quantum dots as yield increasing agents in the aspect of regulating lettuce growth:
(1) Mixing citric acid, taurine and secondary distilled water according to the mass ratio of 1:0.8:5, placing the mixture into a container, stirring and dissolving the mixture, drying the mixture at 60 ℃ until no water exists in the container, transferring the container into a high-temperature oven, preserving heat for 12 hours at 200 ℃, and cooling the container to room temperature to obtain an intermediate product; mixing the intermediate product and deionized water according to the mass ratio of 1:10, adding sodium hydroxide solution until the pH value of the solution is 7, passing through a 0.22 mu m microporous filter membrane, dialyzing in ultrapure water for 24 hours by using a dialysis bag with the molecular weight cutoff of 3kDa, and freezing at-20 ℃ for 24 hours to obtain graphene quantum dots;
(2) Configuring graphene quantum dots into graphene quantum dot suspension with concentration of 3600 mg/L;
(3) After lettuce grows to the stage of four leaves and one heart, uniformly and consistently transplanting seedlings into a cultivation groove filled with nutrient solution for internal planting, spraying lettuce leaves every five days, washing fresh lettuce leaves with deionized water, then uniformly spraying graphene quantum dot suspension on lettuce leaves by using moisture on the surface of a piece of absorbent paper, covering the whole leaf surface and not gathering into water drops to flow down, naturally airing in air, harvesting after 21 days of field planting, measuring lettuce growth and physiological indexes, and carrying out statistical analysis.
Example 3
Application of graphene quantum dots as yield increasing agents in the aspect of regulating lettuce growth:
(1) Mixing citric acid, taurine and secondary distilled water according to the mass ratio of 1:0.75:5, placing the mixture into a container, stirring and dissolving the mixture, drying the mixture at 60 ℃ until no water exists in the container, transferring the container into a high-temperature oven, preserving heat for 11 hours at 190 ℃, and cooling the container to room temperature to obtain an intermediate product; mixing the intermediate product and deionized water according to the mass ratio of 1:10, adding sodium hydroxide solution until the pH value of the solution is 7, passing through a 0.22 mu m microporous filter membrane, dialyzing in ultrapure water for 24 hours by using a dialysis bag with the molecular weight cutoff of 3kDa, and freezing at-20 ℃ for 24 hours to obtain graphene quantum dots;
(2) Preparing graphene quantum dots and deionized water into graphene quantum dot suspension with concentration of 3600 mg/L;
(3) After lettuce grows to the stage of four leaves and one heart, selecting uniform seedling, transplanting the seedling into a cultivation groove filled with nutrient solution for internal planting, spraying lettuce leaves every five days, washing fresh lettuce leaves with deionized water, and drying the moisture on the surface with absorbent paper; uniformly spraying graphene quantum dot suspension onto lettuce leaves, covering the whole leaf surface, enabling the graphene quantum dot suspension not to be aggregated into water drops to flow down, and naturally airing in the air; harvesting after planting for 21 days, measuring lettuce growth and physiological indexes, and carrying out statistical analysis.
Example 4
Application of graphene quantum dots as yield increasing agents in the aspect of regulating lettuce growth:
(1) Mixing citric acid, taurine and secondary distilled water according to the mass ratio of 1:0.75:5, placing the mixture into a container, stirring and dissolving the mixture, drying the mixture at 60 ℃ until no water exists in the container, transferring the container into a high-temperature oven, preserving heat for 11 hours at 190 ℃, and cooling the container to room temperature to obtain an intermediate product; mixing the intermediate product and deionized water according to the mass ratio of 1:10, adding sodium hydroxide solution until the pH value of the solution is 7, passing through a 0.22 mu m microporous filter membrane, dialyzing in ultrapure water for 24 hours by using a dialysis bag with the molecular weight cutoff of 3kDa, and freezing at-20 ℃ for 24 hours to obtain graphene quantum dots;
(2) Preparing graphene quantum dots and deionized water into graphene quantum dot suspension with the concentration of 1200 mg/L;
(3) After lettuce grows to the stage of four leaves and one heart, selecting uniform seedling, transplanting the seedling into a cultivation groove filled with nutrient solution for internal planting, spraying lettuce leaves every five days, washing fresh lettuce leaves with deionized water, and drying the moisture on the surface with absorbent paper; uniformly spraying graphene quantum dot suspension onto lettuce leaves, covering the whole leaf surface, enabling the graphene quantum dot suspension not to be aggregated into water drops to flow down, and naturally airing in the air; harvesting after planting for 21 days, measuring lettuce growth and physiological indexes, and carrying out statistical analysis.
Example 5
Application of graphene quantum dots as yield increasing agents in the aspect of regulating lettuce growth:
(1) Mixing citric acid, taurine and secondary distilled water according to the mass ratio of 1:0.75:5, placing the mixture into a container, stirring and dissolving the mixture, drying the mixture at 60 ℃ until no water exists in the container, transferring the container into a high-temperature oven, preserving heat for 11 hours at 190 ℃, and cooling the container to room temperature to obtain an intermediate product; mixing the intermediate product and deionized water according to the mass ratio of 1:10, adding sodium hydroxide solution until the pH value of the solution is 7, passing through a 0.22 mu m microporous filter membrane, dialyzing in ultrapure water for 24 hours by using a dialysis bag with the molecular weight cutoff of 3kDa, and freezing at-20 ℃ for 24 hours to obtain graphene quantum dots;
(2) Preparing graphene quantum dots and deionized water into a graphene quantum dot suspension with concentration of 2400 mg/L;
(3) After lettuce grows to the stage of four leaves and one heart, selecting uniform seedling, transplanting the seedling into a cultivation groove filled with nutrient solution for internal planting, spraying lettuce leaves every five days, washing fresh lettuce leaves with deionized water, and drying the moisture on the surface with absorbent paper; uniformly spraying graphene quantum dot suspension onto lettuce leaves, covering the whole leaf surface, enabling the graphene quantum dot suspension not to be aggregated into water drops to flow down, and naturally airing in the air; harvesting after planting for 21 days, measuring lettuce growth and physiological indexes, and carrying out statistical analysis.
Example 6
Application of graphene quantum dots as yield increasing agents in the aspect of regulating lettuce growth:
(1) Mixing citric acid, taurine and secondary distilled water according to the mass ratio of 1:0.75:5, placing the mixture into a container, stirring and dissolving the mixture, drying the mixture at 60 ℃ until no water exists in the container, transferring the container into a high-temperature oven, preserving heat for 11 hours at 190 ℃, and cooling the container to room temperature to obtain an intermediate product; mixing the intermediate product and deionized water according to the mass ratio of 1:10, adding sodium hydroxide solution until the pH value of the solution is 7, passing through a 0.22 mu m microporous filter membrane, dialyzing in ultrapure water for 24 hours by using a dialysis bag with the molecular weight cutoff of 3kDa, and freezing at-20 ℃ for 24 hours to obtain graphene quantum dots;
(2) Preparing graphene quantum dots and deionized water into graphene quantum dot suspension with the concentration of 4800 mg/L;
(3) After lettuce grows to the stage of four leaves and one heart, selecting uniform seedling, transplanting the seedling into a cultivation groove filled with nutrient solution for internal planting, spraying lettuce leaves every five days, washing fresh lettuce leaves with deionized water, and drying the moisture on the surface with absorbent paper; uniformly spraying graphene quantum dot suspension onto lettuce leaves, covering the whole leaf surface, enabling the graphene quantum dot suspension not to be aggregated into water drops to flow down, and naturally airing in the air; harvesting after planting for 21 days, measuring lettuce growth and physiological indexes, and carrying out statistical analysis.
Example 7
Application of graphene quantum dots as yield increasing agents in the aspect of regulating lettuce growth:
pure water is used as a spraying agent, after lettuce grows to the stage of four leaves and one heart, uniform and consistent seedlings are selected to be transplanted into a cultivation groove filled with nutrient solution for internal planting, lettuce leaves are sprayed every five days, fresh lettuce leaves are washed by deionized water, then the water on the surface of the lettuce leaves is dried by absorbent paper, pure water is uniformly sprayed on the lettuce leaves, the whole leaf surfaces are covered and do not gather into water drops to flow down, then the lettuce leaves are naturally dried in the air, harvested after 21 days of permanent planting, and the lettuce growth and physiological indexes are measured and statistically analyzed.
Effect example
The results of performance analysis of different concentrations of graphene quantum dots in raw vegetable growth and physiological index using examples 3 to 7 of the present invention are shown in Table 1 below
TABLE 1 growth index and nutrient index evaluation data sheet of different concentrations of graphene quantum dots as yield increasing agent in regulating lettuce growth
The fresh weight of the plant and the length of the leaf can reflect the yield of the crop. From Table 1, it can be seen that the graphene quantum dots have the effect of promoting plant growth as photosynthesis regulator, along with the increase of the concentration of the graphene quantum dots, the fresh weight and the leaf length of lettuce are increased and then reduced, when the concentration of the graphene quantum dots is increased to 3600mg/L, the growth rate of lettuce is fastest, the fresh weight reaches 50.508g, and the leaf length reaches 19cm. When the concentration is increased to 4800mg/L, the lettuce growth rate is slowed down, the fresh weight is 44.947g, which indicates that the graphene quantum dot suspension with the concentration of 3600mg/L can obviously promote the plant growth.
The organic matters formed by photosynthesis account for more than 90% of the dry weight of plants, and the main component is sugar, so that the two index indexes can reflect the nutrition level of crops. From Table 2, it can be seen that the graphene quantum dot has the effect of promoting the accumulation of plant nutrients as the yield-increasing agent, as the concentration of the graphene quantum dot increases, the dry weight and the soluble sugar content of lettuce increase first and then decrease, when the concentration of the graphene quantum dot increases to 3600mg/L, the nutrient accumulation of lettuce is most, the dry weight reaches 17.972g, the soluble sugar content reaches 13.58mg/g, and when the concentration increases to 4800mg/L, the nutrient accumulation of lettuce is most slowed down, the dry weight is 15.764g, which indicates that the graphene quantum dot suspension with the concentration of 3600mg/L can obviously promote the accumulation of plant nutrients.
The following table 2 shows the results of performance analysis of the graphene quantum dots prepared by different ratios of the embodiments 1 to 3 in the growth and physiological indexes of raw vegetables.
Table 2 growth index and nutrition index evaluation data table of graphene quantum dots prepared in different ratios as yield increasing agent in lettuce growth regulation
| Fresh weight of lettuce after harvesting (g) | Lettuce leaf length (cm) after harvesting | |
| Example 1 | 40.22 | 16.3 |
| Example 2 | 35.48 | 15.1 |
| Example 3 | 50.51 | 19.4 |
As can be seen from the comparison of experimental data of example 1 and example 3, the amount of taurine added in example 1 is small, the number of the introduced sulfonic acid groups is small, and the influence on the light absorption of graphene quantum dots is very small, so that the yield increasing agent in example 1 only depends on the self effect of the quantum dots, and has a general effect of promoting lettuce growth; from the comparison of experimental data of example 2 and comparative example 3, it can be found that as the taurine content increases, the sulfonic acid groups on the surface of the graphene quantum dots increase, so that example 2 is formed to take electron absorption as a dominant factor, the light absorption performance of the graphene quantum dots is affected, the absorption ratio of the leaf plants to sunlight is reduced, and lettuce growth is inhibited.
Finally, it should be noted that: the foregoing description is only a preferred embodiment of the present invention, and the present invention is not limited thereto, but it is to be understood that modifications and equivalents of some of the technical features described in the foregoing embodiments may be made by those skilled in the art, although the present invention has been described in detail with reference to the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (8)
1. The water-soluble sulfonic graphene quantum dot yield increasing agent is characterized in that the yield increasing agent is prepared by dissolving a proper amount of graphene quantum dots in deionized water and regulating and controlling the concentration.
2. The water-soluble sulfonate graphene quantum dot yield increasing agent according to claim 1, wherein the graphene quantum dot is sulfonate-modified graphene quantum dot.
3. The water-soluble sulfonic graphene quantum dot yield increasing agent according to claim 2, wherein the preparation method of the graphene quantum dot is as follows: mixing citric acid, taurine and secondary distilled water according to the mass ratio of 1:0.7:5-1:0.8:5, placing the mixture into a container, stirring and dissolving the mixture, drying the mixture at 60 ℃ until no moisture exists in the container, transferring the container into a high-temperature oven, preserving heat for 10-12 hours at 180-200 ℃, and cooling the container to room temperature to obtain an intermediate product; mixing the intermediate product and deionized water according to the mass ratio of 1:10, adding sodium hydroxide solution until the pH value of the solution is 7, passing through a 0.22 mu m microporous filter membrane, dialyzing in ultrapure water for 24 hours by using a dialysis bag with the molecular weight cut-off of 3kDa, and freezing at-20 ℃ for 24 hours.
4. The water-soluble sulfographene quantum dot yield increasing agent according to claim 3, wherein the size of the graphene quantum dot is 2-20 nm.
5. The water-soluble sulfonic graphene quantum dot yield-increasing agent according to claim 1, wherein the concentration of graphene quantum dots in the yield-increasing agent is 0mg/L to 4800mg/L.
6. The use of a water-soluble sulfographene quantum dot yield increasing agent according to claim 1, wherein said yield increasing agent is directly applied to plants.
7. The use of a water-soluble sulfographene quantum dot stimulation agent according to claim 6, wherein the application mode is that the foliar spray is applied to the whole foliar surface and does not gather in a droplet stream, and the spraying time is the plant growth period.
8. The use of a water-soluble sulfographene quantum dot stimulation agent according to claim 6, wherein the plants include, but are not limited to, lettuce, cucumber, corn and other crops with leaves during growth.
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