CN116869009A - Novel plant sterilization disease-resistant activator - Google Patents

Novel plant sterilization disease-resistant activator Download PDF

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Publication number
CN116869009A
CN116869009A CN202310839623.7A CN202310839623A CN116869009A CN 116869009 A CN116869009 A CN 116869009A CN 202310839623 A CN202310839623 A CN 202310839623A CN 116869009 A CN116869009 A CN 116869009A
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CN
China
Prior art keywords
disease
disease resistance
sterilization
plant sterilization
epoxiconazole
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Pending
Application number
CN202310839623.7A
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Chinese (zh)
Inventor
钟航
高燕
刘明津
沈会芳
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Yang Xia
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Guangzhou Yueshun Agricultural Technology Co ltd
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Priority to CN202310839623.7A priority Critical patent/CN116869009A/en
Publication of CN116869009A publication Critical patent/CN116869009A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/82Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with three ring hetero atoms
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/541,3-Diazines; Hydrogenated 1,3-diazines
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/561,2-Diazoles; Hydrogenated 1,2-diazoles
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00Plant growth regulators
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00Fungicides

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  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to a novel plant sterilization disease-resistant activator. The active ingredients of the plant sterilization and disease resistance activation composition are compounded by disease resistance activation active ingredients and sterilization active ingredients, and the disease resistance activation active ingredients are epoxiconazole activated ester; the bactericidal active ingredient is ningnanmycin, berberine or cyproconazole. The active ingredients of the plant sterilization and disease resistance activator are compounded by the disease resistance activation active ingredients and the sterilization active ingredients, and the plant sterilization and disease resistance activator has a synergistic effect on pathogenic bacteria causing plant diseases, and can improve the control effect on the plant diseases compared with a single active ingredient. In addition, the action mechanisms of the disease-resistant active ingredient and the bactericidal active ingredient are different, so that the development of drug resistance of pathogenic bacteria can be effectively delayed, and the problem existing in the application of a single active ingredient can be effectively solved.

Description

Novel plant sterilization disease-resistant activator
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a novel plant sterilization disease-resistant activator.
Background
The epoxiconazole activated ester, also called B2-a, is a green pesticide 'plant disease resistance activator' with novel action mechanism developed by the university of eastern China and the Tongtaihe chemical industry Co-ordination of Jiang Suna. The epoxiconazole activated ester has no bactericidal activity per se, but can activate a plant immune system when being applied to the surface of plants, thereby enhancing the infection to pathogenic bacteria, and is mainly used for preventing cucumber downy mildew and powdery mildew.
In the agricultural production process, long-term application of a chemical agent with a single active ingredient is easy to cause occurrence of disease resistance. The compounding of different bactericidal active ingredient varieties is a convenient and effective method most commonly used for preventing and treating resistant diseases. When different bactericidal active ingredients are compounded, three action types are shown: the compound formulation with synergistic effect can widen the bactericidal spectrum, improve the control effect on diseases and delay the development of drug resistance of diseases, but has few compound formulation with synergistic effect in the actual compound formulation screening process. The inventor finds that when the epoxiconazole activated ester is compounded with the prior medicaments of ningnanmycin, berberine or epoxiconazole and the like, the epoxiconazole activated ester has synergistic effect on powdery mildew (Sphaerotheca fuliginea (Schlecht.) poll) of cucumber powdery mildew within a certain mass ratio range, and no related report on the aspect is seen at present.
Disclosure of Invention
The invention aims to provide a novel plant sterilization disease-resistant activator, which solves the problem of long-term use of a single active ingredient chemical agent.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the active ingredients of the plant sterilization and disease resistance activation composition are compounded by disease resistance activation active ingredients and sterilization active ingredients, and the disease resistance activation active ingredients are epoxiconazole activated ester; the bactericidal active ingredient is ningnanmycin, berberine or cyproconazole.
Preferably, the mass ratio of the epoxiconazole activated ester to the ningnanmycin is 1-5:35-1.
Preferably, the mass ratio of the epoxiconazole activated ester to the berberine is 1-21:28-1.
Preferably, the mass ratio of the epoxiconazole activated ester to the epoxiconazole is 1-12:8-1.
The invention also provides a novel plant sterilization and disease resistance activator, which comprises the plant sterilization and disease resistance activation composition and preparation auxiliary materials.
Preferably, the mass of the plant sterilization disease-resistant activation composition accounts for 0.5-90% of the total mass.
Preferably, the preparation auxiliary materials are selected from one or more of wetting agents, dispersing agents, disintegrating agents, defoamers, thickening agents, preservatives, stabilizers, colorants, fillers and deionized water.
Preferably, the preparation is wettable powder, water dispersible granule, suspending agent or microcapsule suspending agent.
Compared with the prior art, the invention has the following beneficial effects:
the active ingredients of the plant sterilization and disease resistance activator are compounded by the disease resistance activation active ingredients and the sterilization active ingredients, and the plant sterilization and disease resistance activator has a synergistic effect on pathogenic bacteria causing plant diseases, and can improve the control effect on the plant diseases compared with a single active ingredient. In addition, the action mechanisms of the disease-resistant active ingredient and the bactericidal active ingredient are different, so that the development of drug resistance of pathogenic bacteria can be effectively delayed, and the problem existing in the application of a single active ingredient can be effectively solved.
Detailed Description
The following is a clear and complete description of the technical solutions of the present patent.
Potting test
1. Test crop
The cucumber is cultivated in a pot until 2 leaves are in a true leaf period, and each pot is provided with 2 plants, and numbering is reserved.
2. Test pathogenic bacteria
Leaves infected with powdery mildew in cucumber plantations are collected, and pathogenic bacteria are separated and purified in a laboratory, and identified as powdery mildew (Sphaerotheca fuliginea (schlecht.) Poll.) Purpureae.
Inoculating the separated pathogenic bacteria onto the tested crop, washing fresh spores on leaves with tween-80 water solution with mass fraction of 0.05%, filtering with double-layer gauze to obtain 1×10 5 Individual spores/mL of suspension for later use.
3. Test agent
98% of epoxiconazole activated ester crude drug (Nantong Taihe chemical Co., ltd.), 40% of Ningnan mycin mother drug (Sichuan golden bead ecological agriculture technology Co., ltd.), 98% of berberine crude drug (Chengdu Rui biological technology Co., ltd.), 96% of epoxiconazole crude drug (Living Nannong crop protection Co., ltd.)
After the test agent is dissolved, the solution is diluted into a single-agent mother solution by using a tween-80 water solution with the mass fraction of 0.1 percent, a plurality of groups of proportions are arranged, and each single agent and each proportion are respectively provided with 5 mass concentration gradients for standby.
4. Test method (refer to NY/T1156.4-2008 "pesticide indoor biological assay test criterion Bactericide part 4: test potted method for preventing and treating wheat powdery mildew
The liquid medicine is uniformly sprayed on the leaf surfaces of the tested crops until the leaves are all wet, the liquid medicine is naturally dried and then is moved to a constant temperature chamber of 25 ℃ for continuous culture, 10 pots are treated by each liquid medicine with mass concentration, and the treatment of 0.1% Tween-80 aqueous solution is used as a blank control. After the liquid medicine is treated for 4d, the prepared spore suspension is used for spraying and inoculating the tested crops, and the tested crops are moved to a constant temperature chamber of 25 ℃ for continuous culture after natural air drying. And when the leaf disease rate of the blank control reaches more than 80%, the disease conditions of all leaves treated are investigated in a grading way, and the disease index and the prevention and treatment effect of each treatment are calculated.
Wherein, the grading standard is shown in Table 1.
5. Grading standard
Level 0: no disease;
stage 1: the area of the disease spots accounts for less than 5% of the area of the whole leaf;
3 stages: the area of the disease spots accounts for 5-15% of the area of the whole leaf;
5 stages: the area of the disease spots accounts for 15-25% of the area of the whole leaf;
7 stages: the area of the lesion accounts for 25-50% of the area of the whole leaf;
stage 9: the area of the disease spots accounts for 50-75% of the area of the whole leaf;
11 grades: the area of the disease spots accounts for more than 75% of the area of the whole leaf.
6. Data analysis: data statistics analysis is carried out by adopting DPS software to obtain a virulence regression equation of each medicament and virulence EC of the medicament on target bacteria 50 The value was calculated as co-toxicity coefficient (CTC) according to grand cloud Pei method.
7. Measurement results
The synergy of the agents was evaluated based on the calculated co-toxicity coefficient (CTC), CTC.ltoreq.80 being antagonism, CTC.ltoreq.120 being additive, CTC.ltoreq.120 being synergy, the results being shown in tables 1-3.
TABLE 1 toxicity determination results of Furazole activated ester and Ningnan mycin compounded Erysiphe graminis
As can be seen from Table 1, when the epoxiconazole activated ester is compounded with Ningnanmycin, the ratio is between 1 and 5: within the mass ratio of 35-1, the co-toxicity coefficient of the powdery mildew on the target powdery mildew is more than 120, and the synergistic effect is shown.
TABLE 2 toxicity determination results of Furazole activated ester and berberine compounded on Erysiphe graminis
As can be seen from Table 2, when the fluxazole-activated ester is compounded with berberine, the ratio of the fluxazole-activated ester to berberine is 1-21:28-1, the co-toxicity coefficient of the powdery mildew on the target powdery mildew is more than 120, and the synergistic effect is shown.
TABLE 3 toxicity determination results of Furazole activated ester and Furazolam compounded on Eichhornia crassipes
As can be seen from Table 3, when the epoxiconazole activated ester is compounded with the epoxiconazole, the ratio of the epoxiconazole activated ester to the epoxiconazole is between 1 and 12: and within the mass ratio of 8-1, the co-toxicity coefficient of the powdery mildew on the target monofilament shell is more than 120, and the synergistic effect is shown.
In conclusion, when the epoxiconazole activated ester is compounded with the ningnanmycin, the berberine or the epoxiconazole, the epoxiconazole activated ester has a synergistic effect on powdery mildew (Sphaerotheca fuliginea (Schlecht.) poll.) of the cucumber powdery mildew in a certain mass ratio range, and compared with single effective components, the epoxiconazole activated ester can improve the control effect on plant diseases. In addition, the action mechanisms of the disease-resistant active ingredient and the bactericidal active ingredient are different, so that the development of drug resistance of pathogenic bacteria can be effectively delayed.

Claims (8)

1. The plant sterilization and disease resistance activation composition is characterized in that the effective components of the plant sterilization and disease resistance activation composition are compounded by disease resistance activation active components and sterilization active components, and the disease resistance activation active components are epoxiconazole activated ester; the bactericidal active ingredient is ningnanmycin, berberine or cyproconazole.
2. The plant sterilization and disease resistance activation composition according to claim 1, wherein the mass ratio of the epoxiconazole activated ester to the ningnanmycin is 1-5:35-1.
3. The plant sterilization and disease resistance activation composition of claim 1, wherein the mass ratio of the epoxiconazole activated ester to the berberine is 1-21:28-1.
4. The plant sterilization and disease resistance activation composition according to claim 1, wherein the mass ratio of the epoxiconazole activated ester to the epoxiconazole is 1-12:8-1.
5. A novel plant sterilization and disease-resistant activator, which is characterized in that the novel plant sterilization and disease-resistant activator comprises the plant sterilization and disease-resistant activating composition and preparation auxiliary materials as claimed in any one of claims 1 to 4.
6. The novel plant sterilization and disease-resistant activator according to claim 5, wherein the mass of the plant sterilization and disease-resistant activator composition is 0.5-90% of the total mass.
7. The novel plant sterilization and disease-resistant activator according to claim 5, wherein the formulation auxiliary materials are selected from one or more of wetting agents, dispersing agents, disintegrants, defoamers, thickeners, preservatives, stabilizers, colorants, fillers and deionized water.
8. The novel plant sterilization and disease resistance activator according to claim 5, wherein the preparation is wettable powder, water dispersible granule, suspending agent or microcapsule suspending agent.
CN202310839623.7A 2023-07-10 2023-07-10 Novel plant sterilization disease-resistant activator Pending CN116869009A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202310839623.7A CN116869009A (en) 2023-07-10 2023-07-10 Novel plant sterilization disease-resistant activator

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202310839623.7A CN116869009A (en) 2023-07-10 2023-07-10 Novel plant sterilization disease-resistant activator

Publications (1)

Publication Number Publication Date
CN116869009A true CN116869009A (en) 2023-10-13

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202310839623.7A Pending CN116869009A (en) 2023-07-10 2023-07-10 Novel plant sterilization disease-resistant activator

Country Status (1)

Country Link
CN (1) CN116869009A (en)

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Effective date of registration: 20240313

Address after: No. 10-1, Wenqian Sanxiang, Xiyang District, Huahu Town, Huilai County, Jieyang City, Guangdong Province, China, 515226

Applicant after: Yang Xia

Country or region after: China

Address before: No. 147 Shikengzheng Road, Wenquan Town, Conghua District, Guangzhou City, Guangdong Province, 510970

Applicant before: Guangzhou Yueshun Agricultural Technology Co.,Ltd.

Country or region before: China

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