CN116837039A - Method for directly biosynthesizing erythritol by using carbon dioxide and application thereof - Google Patents

Method for directly biosynthesizing erythritol by using carbon dioxide and application thereof Download PDF

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CN116837039A
CN116837039A CN202310785616.3A CN202310785616A CN116837039A CN 116837039 A CN116837039 A CN 116837039A CN 202310785616 A CN202310785616 A CN 202310785616A CN 116837039 A CN116837039 A CN 116837039A
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erythritol
yida
carbon dioxide
directly
biosynthesizing
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王乐
李萌
李忠增
孙罩俊
曹新花
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Henan University of Technology
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

Abstract

The invention provides a method for directly biosynthesizing erythritol by utilizing carbon dioxide and application thereof, belonging to the field of bioengineering and synthetic biology. The invention utilizes a homologous recombination system to transform, integrates phosphatase and erythrose reductase genes related to erythritol synthesis into the chromosome of wild blue algae, thereby obtaining the product which can directly utilize CO 2 And the genetically engineered blue algae can efficiently produce erythritol. The invention not only can directly utilize carbon dioxide as raw material to effectively solve the raw material problem, but also has safe production, so that the invention is environment-friendly, low in cost and applicableContinuous synthesis of erythritol is a reality.

Description

Method for directly biosynthesizing erythritol by using carbon dioxide and application thereof
Technical Field
The invention belongs to the field of bioengineering and synthetic biology, and particularly relates to a method for biosynthesizing erythritol by using direct carbon dioxide and application thereof.
Background
Currently, the main carbon sources for the microbial production of erythritol are glucose and glycerol. Compared with the two, when glucose is used as a carbon source, the erythritol has higher yield, but more byproducts; while glycerol is used as a substrate, although the yield is slightly lower, the byproducts are fewer, and the ratio of available erythritol and byproducts is higher. Jeya et al utilized different carbon sources such as glucose, glycerol, etc. against strainsP. tsukubaensisComparing KN75 to erythritol, it was found that the concentration of erythritol produced was highest when glucose was used as the carbon source. Mironczuk et al fermentation with glycerol as substrateY. lipolyticaProduction of erythritol by Wratislava K1 can result in a byproduct content of less than 10%. However, the production of erythritol using glucose and glycerol as substrates is costly, and thus the discovery and utilization of novel carbon sources that can replace both are of interest to researchers. With the development of synthetic biology and metabolic engineering technology, blue algae are regarded as a microorganism photosynthetic platform with great potential for developing photosynthetic cell factories to realize CO 2 One-stop directional conversion to biofuels and bio-based chemicals. Sugar substances are a very representative blue algae CD-ROM carbon fixation synthetic product. CO has been turned on by remodelling of the photosynthetic metabolic network 2 Conversion routes to various monosaccharides, oligosaccharides, polysaccharides and sugar alcohol products, e.g., wu et al introduce different sucrose isomerase enzymes into cyanobacteriaSynechococcus elongatus PCC 7942, the synthesis and accumulation of isomaltulose in recombinant strains is realized. Li Wei et al will encode xylitol phosphate dehydrogenase genexpdhIntroduction into blue algaeSynechococcus elongatus In PCC 7942, a pentose phosphate extension pathway is constructed in blue algae, and CO utilization is successfully constructed 2 And (3) a blue algae engineering strain for producing xylitol. Madsen et al fromMicromonas pusillaThe fusion enzymes (comprising mannitol-1-phosphate dehydrogenase and mannitol-1-phosphatase) in E.coli and cyanobacteria, respectivelySynechococcusHeterologous expression in sp, PCC 7002, the synthesis of mannitol is achieved. But takes blue algae as a photosynthetic platform and utilizes CO 2 The fermentative production of erythritol for the sole carbon source has been studied.
The inventionAccording to the method, related genes for synthesizing erythritol are introduced into blue algae through a genetic engineering modification strategy, so that the research on erythritol synthesis is realized, and the problem that currently added precursor substances or raw materials are expensive is relieved. Such construction of photosynthetic platforms utilizes CO 2 And the strategy of solar energy enables environment-friendly, low-cost and sustainable synthesis of erythritol to be realized, and the way of producing compounds by utilizing a photosynthetic platform is widened.
Disclosure of Invention
The invention provides a method for biosynthesis of erythritol by directly utilizing carbon dioxide as a raw material and application thereof, which not only can effectively solve the raw material problem, but also is safe to produce, environment-friendly, low in cost and sustainable, and provides a new way for effectively improving biosynthesis of erythritol.
The first object of the invention is to provide a method for directly biosynthesizing erythritol by using carbon dioxide, which comprises the following specific steps:
firstly, fusing a gene yidA and SD sequence of encoding phosphatase with a gene ER of encoding erythrose reductase, then inserting a fusion fragment yidA-SD-ER into a plasmid pAM2991 to construct a recombinant plasmid pAM-yidA-SD-ER, and finally integrating the recombinant plasmid pAM-yidA-SD-ER on a chromosome of wild blue algae, thereby obtaining the genetically engineered blue algae capable of producing erythritol.
Further, the gene yidA encoding phosphatase is derived fromEscherichia coliK-12, the SD sequence is derived from plasmid pETDuet-1, and the gene ER encoding erythrose reductase is derived fromCandida magnoliaeDSM 70638。
Further, the nucleotide sequence of the fusion fragment yidA-SD-ER is shown as SEQ ID NO. 1.
Further, the fusion fragment yidA-SD-ER was obtained by using an overlay PCR.
Further, the genetically engineered cyanobacteria takes plasmid pAM2991 as an expression vector.
Further, the plasmid pAM2991 contains a spectinomycin resistance selection gene and IPTG-induced P trc A promoter.
Further, the transformation mode of the recombinant plasmid is a natural transformation method, and the exogenous gene is expressed in the following waySynechococcus elongatus Homologous exchange recombination is performed at the position of the Neutral Site (NS) of the PCC 7942 chromosome.
Further, the saidSynechococcus elongatus The PCC 7942 chromosome NS is the Neutral Site I (NSI) which is also present in plasmid pAM2991.
A second object of the present invention is to provide the use of a method for biosynthesis of erythritol directly using carbon dioxide for the production of erythritol. The method comprises the following specific steps:
selecting transformant PM, placing in proper culture temperature and illumination intensity, and performing continuous ventilation experiment by using Synechococcus elongatusPCC 7942 served as a blank. Culturing genetically engineered blue algae PM and Synechococcus elongatusOD of PCC 7942 730 Adding 50-100 μl of 100 mmol/L IPTG to induce, sampling every 5 days, continuously sampling for tens of days, adding BG-11 liquid culture medium to 50-100 mL before sampling, sampling two tubes each time, one tube for measuring erythritol yield, and the other tube for measuring OD 730 Values.
Further, the culture temperature is 25-30 ℃.
Further, the illumination intensity is 1500-2500 lux.
Further, the gas used in the continuous aeration is 1-5% CO 2 And 95-99% air.
Further, the formula of the BG-11 culture medium is as follows: naNO 3 （1.5-7.5 g），K 2 HPO 4 （0.04-0.35 g），MgSO 4 ·7H 2 O（0.075-0.5 g），CaCl 2 ·2H 2 O（0.036-0.18 g），Citric acid（6×10 -3 -6×10 -2 g），Ferric ammonium citrate（6×10 -3 -6×10 -2 g），EDTANa 2 （5×10 -4 -5×10 -3 g），Na 2 CO 3 （0.02-1 g），A5（Trace mental solution）：H 3 PO 4 （2.9×10 -3 -2.9×10 -2 g），MnCl 2 ·4H 2 O（1.9×10 -3 -1.9×10 -2 g），ZnSO 4 ·7H 2 O（2.2×10 -4 -2.2×10 -3 g），Na 2 MoO 4 ·2H 2 O（3.9×10 -4 -3.9×10 -3 g），CuSO 4 ·5H 2 O（8×10 -5 -8×10 -4 g），Co(NO 3 ) 2 ·6H 2 O（5×10 -5 -5×10 -4 g) The solid culture medium is required to be added with 2% of agar powder, distilled water is finally used for fixing the volume to 1L, 1 mol/L NaOH or 1 mol/L HCl is used for adjusting the pH value to 7.1, and the culture medium is sterilized for 20 min at 121 ℃.
Further, the method for detecting the erythritol yield adopts GB 5009.279-2016 and high performance liquid chromatography.
Further, the conditions of the high performance liquid chromatography are as follows: shimadzu LC-2030C liquid chromatograph, differential display: RID-20A, column: waters sugam-pakl, mobile phase: ultrapure water was used at a flow rate of 0.5. 0.5 g/L and a column temperature of 80 ℃.
The reagents and materials used above are all commercially available unless otherwise specified.
Advantageous effects
1. The invention carries out genetic modification on blue algae, reconstructs the synthetic route of erythritol in the blue algae, and the obtained genetically engineered blue algae can convert solar energy and CO 2 Directly converted into erythritol. The invention solves the problem of raw material sources, and has safe production, so that the method is environment-friendly, low in cost and capable of continuously synthesizing erythritol.
2. In the specific embodiment of the invention, after the blue algae engineering bacteria are cultured for tens of days, the blue algae engineering bacteria can produce more than 1.5 g/L erythritol, which opens up a new technology for the production of erythritol and has good industrial application prospect.
Drawings
FIG. 1 shows the map of the recombinant plasmid pAM-yidA-SD-ER of example 1 of the present invention.
FIG. 2 shows the result of the cleavage verification of the recombinant plasmid pAM-yidA-SD-ER according to example 1 of the present invention. M represents DNA Marker, L1 represents single cleavage verification of plasmid pAM2991, L2 represents single cleavage verification of recombinant plasmid pAM-yidA-SD-ER, L3 represents double cleavage verification of plasmid pAM2991, and L4 represents double cleavage verification of recombinant plasmid pAM-yidA-SD-ER.
FIG. 3 shows a genetic engineering strain PM and a blank control according to example 2 of the present inventionSynechococcus elongatus Graph of erythritol yield of PCC 7942 over time.
Detailed Description
Specific embodiments of the present invention will be described in further detail below with reference to examples. The following examples are intended to further illustrate the invention and are not intended to limit the scope of the invention. The related content and the modifications thereof belong to the protection scope of the invention.
Blue algaeSynechococcus elongatusPCC 7942 is often used as a model strain for relevant experimental studies. Plasmid pAM2991 for integration into a host cell by homologous recombinationSynechococcus elongatus The chromosome of PCC 7942 expresses the gene of interest.
The PCR amplification systems involved in the examples were all 25-50. Mu.L:
①5×PrimeSTAR Buffer（Mg 2+ plus): 5-10 mu L; (2) dNTP mix: 2-4 mu L; (3) upstream primer F (10 [ mu ] mol/L): 0.5-1. Mu.L; (4) downstream primer R (10 [ mu ] mol/L): 0.5-1. Mu.L; (5) genome template (100-200 ng/μl): 0.5-1. Mu.L; (6) PrimeSTAR HS DNA Polymerase: 0.25-0.5. Mu.L; (7) adding Sterilized ddH 2 O was made up to 25-50. Mu.L.
PCR amplification conditions:
(1) 98 ℃ C: 3 min; (2) 98 ℃ C: 15 s; (3) annealing temperature (depending on the primer pair case): 15 s; (4) 72 ℃ C:: 1 kb/min (return (2)) for 10-30 cycles total; (5) 72 ℃ C:: 3 min; (6) 4 ℃ C:: infinity.
Example 1: construction of erythritol-producing genetically engineered blue algae
Construction of recombinant plasmid pAM-yidA-SD-ER
Extraction ofEscherichia coliGenomic DNA of K-12;
the sequence of the phosphatase corresponding gene yidA is shown as SEQ ID NO.2, a primer pair is designed and synthesized, the sequence of an upstream primer yidA-F1 is shown as SEQ ID NO.4, and the sequence of a downstream primer yidA-R1 is shown as SEQ ID NO. 5;
performing PCR amplification by using the genome DNA of the step (1) as a template and using the primer synthesized in the step (2) to obtain a DNA fragment of the phosphatase corresponding to the gene yidA, wherein the reaction system and the conditions are as described above;
extraction ofCandida magnoliaeGenomic DNA of DSM 70638;
the sequence of the erythrose reductase corresponding gene ER is shown as SEQ ID NO.3, a primer pair is designed and synthesized, the sequence of an upstream primer ER-F1 is shown as SEQ ID NO.6, and the sequence of a downstream primer ER-R1 is shown as SEQ ID NO. 7;
performing PCR amplification by using the genome DNA of the step (4) as a template and using the primer synthesized in the step (5) to obtain a DNA fragment of the erythrose reductase corresponding gene ER, wherein the reaction system and the conditions are as described above;
designing and synthesizing an overlay PCR primer pair, wherein the sequence of an upstream primer yidA-F2 of the yidA gene is shown as SEQ ID NO.8, and the sequence of a downstream primer yidA-R2 is shown as SEQ ID NO. 9. The upstream primer ER-F2 of ER gene is shown as SEQ ID NO.10, and the downstream primer ER-R2 is shown as SEQ ID NO. 11;
using the yidA gene obtained in the step (3) as a template, and performing PCR amplification by using the primers yidA-F2 and yidA-R2 synthesized in the step (7) to obtain a primer with an enzyme cutting siteEcoRI, SD sequence and fragment yidA overlapped with 5' end of ER gene + The reaction system and conditions are shown in the specification. Using ER gene obtained in the step (6) as a template, and performing PCR amplification by using the primers ER-F2 and ER-R2 synthesized in the step (7) to obtain a primer sequence with overlapping sequence with 3' end of yidA gene, SD sequence and enzyme cutting siteBamFragment ER of HI + The reaction system and the conditions are as described above;
the fragment yidA obtained in step (8) is subjected to + And fragment ER + As a template, PCR amplification is carried out without adding primers to obtain fusion fragment yidA-SD-ER, and the reaction system and the conditions are as described above;
diluting the fusion fragment yidA-SD-ER obtained in the step (9) by 5-10 times, carrying out PCR amplification by using the diluted fusion fragment yidA-SD-ER as a template and using the primers yidA-F2 and ER-R2 synthesized in the step (7) to obtain a large number of fusion fragments yidA-SD-ER, wherein the reaction system and the conditions are as described above;
the plasmid pAM2991 and the fusion fragment yidA-SD-ER obtained in step (10) were treated with restriction enzymes, respectivelyEcoRI, and RI systemBamHI was double digested and the digested plasmid and fusion fragment were recovered using AxyPrep DNA Gel Extraction Kit. The amount of the vector fragment and the PCR product fragment required was calculated according to the molar ratio of the recovered vector fragment to the recovered PCR product fragment of 1:3-6, and then the recombinant plasmid pAM-yidA-SD-ER was obtained by ligating overnight at 4℃using T4 ligase from Noruzan.
The recombinant plasmid pAM-yidA-SD-ER obtained in the step (11) was sent to Shanghai Biotechnology Co., ltd for sequencing. The sequencing results were aligned to GeneBank.
Primer sequence (SEQ ID NO. 4-11)
yidA-F1：5'-ATGGCTATTAAACTCATTGCTATCGA-3'
yidA-R1：5'-TTAATTCAGCACATACTTCTCAATAGCA-3'
ER-F1：5'-ATGTCTTCGACCTACACCCTTACTC-3'
ER-R1：5'-TCACCGTCTTGCTAGCGCG-3'
yidA-F2：5'-ccggaattcATGGCTATTAAACTCATTGC-3'
yidA-R2：5'-CGAAGACATggtatatctccttcttTTAATTCAGCACATACTT-3'
Wherein the base sequence indicated by underline in the lower case letter is the cleavage siteEcoThe base sequence of RI, lower case letter not underlined, is the SD sequence.
ER-F2：5'-CTGAATTAAaagaaggagatataccATGTCTTCGACCTACACC-3'
ER-R2：5'-gcgggatccTCACCGTCTTGCTA-3'
Wherein the base sequence of the lowercase letter not underlined is the SD sequence, and the base sequence of the lowercase letter underlined is the cleavage siteBamHI。
Construction of genetically engineered cyanobacteria
Transforming blue algae strains: collecting blue algae cells in logarithmic phase 5-10 mL, centrifuging at 4000-5000 rpm/min for 5-10 min, collecting thallus, washing with sterile 10 mM NaCl once, and re-suspending with 5-10 mL BG-11 liquid culture medium. Taking 500-750 mu L of bacterial liquid into a 1.5 mL centrifuge tube, adding recombinant plasmid with the final concentration of 100-200 ng/mL, uniformly mixing, and culturing at 28-30 ℃ in a dark place for 12-16 hours. And then, the mixture of blue algae cells and recombinant plasmids is coated on a BG-11 solid medium containing 20-30 mug/mL spectinomycin, and the blue algae cells and the recombinant plasmids are placed in a 28-30 ℃ under the illumination intensity of 2000-2200lux for continuous illumination culture until single colonies appear on the solid medium.
(2) Obtaining genetically engineered blue algae: picking the transformant, placing the transformant in 5-10 mL BG-11 liquid culture medium containing 20 mug/mL spectinomycin, and continuously carrying out illumination culture for 10-12 days at 28-30 ℃ under the illumination intensity of 2000-2200 lux. And then extracting genome DNA of the genetically engineered blue-green algae by adopting an Ezup column type bacterial genome DNA extraction kit, and carrying out PCR verification and sequencing verification by utilizing amplification primers yidA-F2 and ER-R2 of exogenous genes on the recombinant plasmid to obtain the genetically engineered blue-green algae PM.
Example 2: production of erythritol by using genetically engineered blue algae PM
(1) Culturing recombinant strains: selecting transformant PM, placing at 28-30deg.C under 2000-2200lux illumination intensity, and performing continuous ventilation experiment by using Synechococcus elongatusPCC 7942 served as a blank. Culturing genetically engineered blue algae PM and Synechococcus elongatusOD of PCC 7942 730 Adding 50-100 μl of 100 mmol/L IPTG to induce, sampling every 5 days for 30 days, adding BG-11 liquid culture medium to 50-100 mL before sampling, sampling two tubes each time, one tube for measuring erythritol yield, and the other tube for measuring OD 730 Values.
(2) Sample treatment: heating the sample obtained in the step (1) at 95-100 ℃ for 10-15 min, centrifuging for 10-15 min at 4 ℃ and 10000-12000 rpm/min, filtering with a water phase special filter membrane, and measuring at 4 ℃.
(3) Sample detection: and (3) detecting the sample prepared in the step (2) by using a high performance liquid chromatography, wherein the peak-out time of the erythritol standard product is 13.1 min, the peak value of the genetically engineered blue algae PM appears at the peak time, the obtained sample is determined to be erythritol, and more than 1.5 g/L of erythritol can be accumulated in tens of days (figure 3). Blank control
Synechococcus elongatus
No peak in PCC 7942 occurred at this time, nor was erythritol produced detected within 30 days (fig. 3). The conditions of the high performance liquid chromatography are as follows: shimadzu LC-2030C liquid chromatograph, differential display: RID-20A, column: waters sugam-pakl, mobile phase: ultrapure water was used at a flow rate of 0.5. 0.5 g/L and a column temperature of 80 ℃.

Claims (5)

1. A method for directly biosynthesizing erythritol by using carbon dioxide is characterized in that firstly, a gene yidA and SD sequence for encoding phosphatase are fused with a gene ER for encoding erythritol reductase, then the fusion fragment yidA-SD-ER is inserted into a plasmid pAM2991 to construct a recombinant plasmid pAM-yidA-SD-ER, and finally, the recombinant plasmid pAM-yidA-SD-ER is integrated on a chromosome of wild type blue algae, so that genetically engineered blue algae capable of producing erythritol can be obtained.
2. The method for directly biosynthesizing erythritol by using carbon dioxide according to claim 1, wherein the SD sequence is derived from plasmid petdet-1.
3. The method for directly biosynthesizing erythritol by using carbon dioxide according to claim 1, wherein the nucleotide sequence of the fusion fragment yidA-SD-ER is shown in SEQ ID No. 1.
4. The method for directly biosynthesizing erythritol by using carbon dioxide according to claim 1, wherein the wild-type cyanobacteria isSynechococcus elongatus PCC 7942。
5. Use of a method according to any one of claims 1-4 for the biosynthesis of erythritol directly using carbon dioxide.
CN202310785616.3A 2023-06-29 2023-06-29 Method for directly biosynthesizing erythritol by using carbon dioxide and application thereof Pending CN116837039A (en)

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