CN116829719A - 蛋白质脱酰胺酶 - Google Patents

蛋白质脱酰胺酶 Download PDF

Info

Publication number
CN116829719A
CN116829719A CN202280012194.XA CN202280012194A CN116829719A CN 116829719 A CN116829719 A CN 116829719A CN 202280012194 A CN202280012194 A CN 202280012194A CN 116829719 A CN116829719 A CN 116829719A
Authority
CN
China
Prior art keywords
ala
seq
leu
ser
glu
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202280012194.XA
Other languages
English (en)
Chinese (zh)
Inventor
松原宽敬
日浦惠太
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amano Enzyme Inc
Original Assignee
Amano Enzyme Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amano Enzyme Inc filed Critical Amano Enzyme Inc
Publication of CN116829719A publication Critical patent/CN116829719A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
    • C12Y305/01044—Protein-glutamine glutaminase (3.5.1.44)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/101—Plasmid DNA for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/20—Flavobacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
CN202280012194.XA 2021-02-03 2022-02-03 蛋白质脱酰胺酶 Pending CN116829719A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2021016088 2021-02-03
JP2021-016088 2021-02-03
PCT/JP2022/004322 WO2022168927A1 (ja) 2021-02-03 2022-02-03 タンパク質脱アミド酵素

Publications (1)

Publication Number Publication Date
CN116829719A true CN116829719A (zh) 2023-09-29

Family

ID=82741600

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202280012194.XA Pending CN116829719A (zh) 2021-02-03 2022-02-03 蛋白质脱酰胺酶

Country Status (5)

Country Link
US (1) US20240124912A1 (https=)
EP (1) EP4289943A4 (https=)
JP (1) JPWO2022168927A1 (https=)
CN (1) CN116829719A (https=)
WO (1) WO2022168927A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN118234866A (zh) * 2021-11-22 2024-06-21 天野酶制品株式会社 蛋白质脱酰胺酶
EP4650443A1 (en) * 2024-05-13 2025-11-19 Novozymes A/S Stabilized liquid deamidase compositions

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3609648B2 (ja) * 1998-06-04 2005-01-12 天野エンザイム株式会社 新規蛋白質脱アミド酵素、それをコードする遺伝子、その製造法並びにその用途
US6251651B1 (en) * 1998-06-04 2001-06-26 Amano Pharmaceutical Co., Ltd. Protein-deamidating enzyme, gene encoding the same, production process therefor, and use thereof
JP3696500B2 (ja) * 1999-12-03 2005-09-21 天野エンザイム株式会社 新規蛋白質脱アミド酵素、それを生産する微生物、それをコードする遺伝子、その製造法及び用途
WO2020176469A1 (en) * 2019-02-26 2020-09-03 Amano Enzyme Usa Co., Ltd. Stable protein formulations

Also Published As

Publication number Publication date
US20240124912A1 (en) 2024-04-18
EP4289943A4 (en) 2025-04-02
JPWO2022168927A1 (https=) 2022-08-11
WO2022168927A1 (ja) 2022-08-11
EP4289943A1 (en) 2023-12-13

Similar Documents

Publication Publication Date Title
CN1871351B (zh) 一种新的真菌蛋白及其编码核酸
KR100855519B1 (ko) 신규 펩타이드 신타제 유전자
CN116829719A (zh) 蛋白质脱酰胺酶
JPH10508475A (ja) トリペプチジルアミノペプチダーゼ
CN101918551A (zh) 草酸脱羧酶的生产中利用的重组表达质粒载体及重组菌、以及重组草酸脱羧酶的生产方法
Kino et al. Dipeptide synthesis by L-amino acid ligase from Ralstonia solanacearum
CN111132556A (zh) 用于对蛋白进行水解的多肽、用途和方法
CN117425729A (zh) 漆酶
US20220162619A1 (en) Preparation of wheat cysteine protease triticain-alpha produced in soluble form and method of producing same
US20100279334A1 (en) Cyclodipeptide synthases (cdss) and their use in the synthesis of linear dipeptides
KR20080108921A (ko) 살조활성 단백질분해효소, 이를 코딩하는 유전자 및 이를포함하는 살조제제
US7601807B2 (en) Protease, DNA encoding the same, and method for manufacturing protease
US20120264191A1 (en) Protein and dna sequence encoding a cold adapted-subtilisin-like activity
Maruyama et al. Prolyl endopeptidase inhibitory activity of peptides in the repeated sequence of various proline-rich proteins
EP1036843A1 (en) DNA molecule encoding an aminopeptidase, and method of producing the aminopeptidase
JP3518868B2 (ja) バシラスリケニフォーミス菌株から由来したアミノペプチダーゼ及び天然蛋白質の製造方法
JP3493400B2 (ja) 新規なアミノ末端保護基遊離酵素
CN117321207A (zh) 漆酶
CN114540263A (zh) 一种耐高温羧肽酶在枯草芽孢杆菌中的构建方法及其在低苦味植物低聚肽中的应用
KR20110086711A (ko) 봉입체 형성 단백질의 제조방법
EP1404829B1 (en) Novel aminopeptidase derived from bacillus licheniformis, gene encoding the aminopeptidase, expression vector containing the gene, transformant and method for preparation thereof
JP2007319063A (ja) ジペプチドの製造方法
CN117535273B (zh) 温敏型碱性蛋白酶变体及其应用
CN118234866A (zh) 蛋白质脱酰胺酶
KR100365838B1 (ko) 브레비바실러스 보스테렌시스 ｂｃｓ-1 에서 유래한 신규내열성 ｄ-입체특이적 디펩티다아제 및 이를 이용한ｄ-아미노산 함유 펩타이드의 합성 방법

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination