CN116829126A - 水凝胶合成物及其使用方法 - Google Patents

水凝胶合成物及其使用方法 Download PDF

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Publication number
CN116829126A
CN116829126A CN202180083084.8A CN202180083084A CN116829126A CN 116829126 A CN116829126 A CN 116829126A CN 202180083084 A CN202180083084 A CN 202180083084A CN 116829126 A CN116829126 A CN 116829126A
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China
Prior art keywords
cells
intestinal
val pro
cell
matrix
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Pending
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CN202180083084.8A
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English (en)
Chinese (zh)
Inventor
莎拉·海尔肖恩
莱利·苏哈尔
丹尼尔·亨特
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Leland Stanford Junior University
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Leland Stanford Junior University
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Publication of CN116829126A publication Critical patent/CN116829126A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0679—Cells of the gastro-intestinal tract
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0012—Cell encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/905—Hyaluronic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/998—Proteins not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/80—Hyaluronan
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2537/00—Supports and/or coatings for cell culture characterised by physical or chemical treatment
    • C12N2537/10—Cross-linking

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicinal Preparation (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202180083084.8A 2020-11-06 2021-11-03 水凝胶合成物及其使用方法 Pending CN116829126A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063110667P 2020-11-06 2020-11-06
US63/110,667 2020-11-06
PCT/US2021/057925 WO2022098777A1 (en) 2020-11-06 2021-11-03 Hydrogel compositions and methods of use thereof

Publications (1)

Publication Number Publication Date
CN116829126A true CN116829126A (zh) 2023-09-29

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180083084.8A Pending CN116829126A (zh) 2020-11-06 2021-11-03 水凝胶合成物及其使用方法

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Country Link
US (1) US20230365940A1 (https=)
EP (1) EP4240327A4 (https=)
JP (1) JP2023548508A (https=)
KR (1) KR20230104219A (https=)
CN (1) CN116829126A (https=)
AU (1) AU2021376143A1 (https=)
CA (1) CA3196621A1 (https=)
IL (1) IL302453A (https=)
WO (1) WO2022098777A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI131721B1 (en) * 2023-09-20 2025-10-16 Teknologian Tutkimuskeskus Vtt Oy Structural protein, medical product, electrospun filament, photonic crystals, metamaterial, thermosensitive glass and method for manufacturing a product
WO2026015902A1 (en) * 2024-07-12 2026-01-15 The Regents Of The University Of California Synthetic matrix for stem cell encapsulation

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101965357A (zh) * 2007-08-15 2011-02-02 特拉维夫大学拉莫特有限公司 多肽、基质、水凝胶以及使用它们进行组织再生和修复的方法
KR20130047404A (ko) * 2011-10-31 2013-05-08 재단법인대구경북과학기술원 엘라스틴 유사 인공 세포외 기질을 이용한 3차원 세포 군집체의 형성 방법과 이를 기반으로 마련된 3차원 세포 군집체

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018144966A1 (en) * 2017-02-06 2018-08-09 The Board Of Trustees Of The Leland Stanford Junior University Bioconjugation methods for targeted in situ therapeutic delivery

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101965357A (zh) * 2007-08-15 2011-02-02 特拉维夫大学拉莫特有限公司 多肽、基质、水凝胶以及使用它们进行组织再生和修复的方法
KR20130047404A (ko) * 2011-10-31 2013-05-08 재단법인대구경북과학기술원 엘라스틴 유사 인공 세포외 기질을 이용한 3차원 세포 군집체의 형성 방법과 이를 기반으로 마련된 3차원 세포 군집체

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DANQING ZHU ET AL,: "Elastin-like Protein-Hyaluronic acid (ELP-HA) Hydrogels with Decoupled Mechanical and Biochemical cues for Cartilage Regeneration", BIOMATERIALS., vol. 127, 3 May 2017 (2017-05-03), pages 132 - 140 *
HUIYUAN WANG ET AL.: "Covalently Adaptable Elastin-Like Protein–Hyaluronic Acid (ELP–HA) Hybrid Hydrogels with Secondary Thermoresponsive Crosslinking for Injectable Stem Cell Delivery", ADVANCED FUCTIONAL MATERIALS, vol. 27, no. 28, 19 May 2017 (2017-05-19), pages 1605609 *

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Publication number Publication date
WO2022098777A1 (en) 2022-05-12
IL302453A (en) 2023-06-01
CA3196621A1 (en) 2022-05-12
AU2021376143A1 (en) 2023-06-08
US20230365940A1 (en) 2023-11-16
KR20230104219A (ko) 2023-07-07
JP2023548508A (ja) 2023-11-17
EP4240327A1 (en) 2023-09-13
EP4240327A4 (en) 2025-03-05

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