CN116813406A - Biological bacterial fertilizer and application thereof in combination with microplastic in enhancing carbon fixation capacity of soil - Google Patents
Biological bacterial fertilizer and application thereof in combination with microplastic in enhancing carbon fixation capacity of soil Download PDFInfo
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- CN116813406A CN116813406A CN202310776389.8A CN202310776389A CN116813406A CN 116813406 A CN116813406 A CN 116813406A CN 202310776389 A CN202310776389 A CN 202310776389A CN 116813406 A CN116813406 A CN 116813406A
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- bacillus
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- 230000001580 bacterial effect Effects 0.000 title claims abstract description 81
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- 229910052799 carbon Inorganic materials 0.000 title claims abstract description 33
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 32
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- 238000000855 fermentation Methods 0.000 claims abstract description 37
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- 239000005416 organic matter Substances 0.000 claims abstract description 36
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 26
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 26
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- 239000007788 liquid Substances 0.000 claims abstract description 10
- 239000000969 carrier Substances 0.000 claims abstract 2
- 239000000203 mixture Substances 0.000 claims description 25
- 239000000618 nitrogen fertilizer Substances 0.000 claims description 21
- 239000002686 phosphate fertilizer Substances 0.000 claims description 19
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 18
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims description 18
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 18
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 18
- 239000011575 calcium Substances 0.000 claims description 18
- 229910052791 calcium Inorganic materials 0.000 claims description 18
- 239000011777 magnesium Substances 0.000 claims description 18
- 229910052749 magnesium Inorganic materials 0.000 claims description 18
- 229940072033 potash Drugs 0.000 claims description 18
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims description 18
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- 239000011573 trace mineral Substances 0.000 claims description 18
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- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical group O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 8
- 239000010457 zeolite Substances 0.000 claims description 8
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
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- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 5
- 239000001110 calcium chloride Substances 0.000 claims description 5
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 5
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- 238000001694 spray drying Methods 0.000 claims description 4
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- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical group [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
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- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 description 2
- KMQAPZBMEMMKSS-UHFFFAOYSA-K calcium;magnesium;phosphate Chemical compound [Mg+2].[Ca+2].[O-]P([O-])([O-])=O KMQAPZBMEMMKSS-UHFFFAOYSA-K 0.000 description 2
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- 241000207199 Citrus Species 0.000 description 1
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- 229910000388 diammonium phosphate Inorganic materials 0.000 description 1
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- 229910000021 magnesium carbonate Inorganic materials 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
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- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
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- 235000010333 potassium nitrate Nutrition 0.000 description 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Fertilizers (AREA)
Abstract
The invention discloses a biological bacterial fertilizer and application of the biological bacterial fertilizer and microplastic combined in enhancing soil carbon fixation capacity. The raw materials of the biological bacterial fertilizer comprise microbial agents and organic matter carriers; the strain in the microbial agent is at least one of bacillus belicus, bacillus methylotrophicus and bacillus licheniformis; the organic matter carrier comprises honey fermentation liquid and animal feces fermentation product. The invention provides a method for improving the carbon fixing capability of soil by combining an organic biological bacterial fertilizer with micro-plastics, which has the characteristics of environmental protection, sustainability and high efficiency; the method can effectively improve the pH value, the SOC and the OM of the soil, thereby generating better comprehensive benefit.
Description
Technical Field
The invention relates to the technical field of agricultural ecology, in particular to a biological bacterial fertilizer and application of the biological bacterial fertilizer and microplastic combined in enhancing carbon fixation capacity of soil.
Background
Soil carbon sequestration refers to the conversion of carbon dioxide already present in the atmosphere into a stable carbon-containing compound by combination and long-term storage in the soil. The soil carbon fixation can provide a cheap environment protection technology for our modern society, can effectively reduce the emission of greenhouse gases, and helps human beings to cope with climate change. The carbon fixation capacity of the soil is improved, and the method has important significance for improving the carbon retention and sink enhancement capacity of an ecological system, guaranteeing global ecological safety and food safety and realizing a double-carbon target.
At present, two modes of physical carbon fixation and biological carbon fixation exist. Physical carbon sequestration is the long-term storage of carbon dioxide in mined oil and gas wells, coal seams and deep seas; biological carbon sequestration is considered as the most promising method for global warming mitigation, and carbon sequestration using terrestrial ecosystems is the most economically viable and environmentally friendly way to mitigate the rise in atmospheric carbon dioxide concentration.
However, at present, soil carbon sequestration faces a number of challenges such as soil degradation, nutrient loss, reduced microbial activity, etc., which can lead to accelerated decomposition of soil organic matter, reduced carbon sequestration capacity and stability.
Biological bacterial fertilizer is an organic fertilizer which utilizes microorganisms or metabolites thereof to improve soil fertility and plant growth. The biological bacterial fertilizer can improve the quantity and activity of beneficial microorganisms in soil, promote the interaction between plant root systems and microorganisms, and increase the absorption and utilization of nutrients and moisture by plants, thereby improving photosynthesis and yield of plants. Meanwhile, the biological bacterial fertilizer can also inhibit soil-borne diseases and pests, and reduce the use of pesticides.
Microplastic refers to plastic particles with diameters smaller than 5 mm, and is a main carrier causing pollution. Microplastic can be classified into primary microplastic and secondary microplastic. The primary microplastic is micron-sized plastic particles prepared in production and is commonly used in industrial manufacture, personal care product production and the like; the secondary micro plastic is generally formed by splitting and reducing the volume of large plastic garbage through physical, chemical and biological processes, and is derived from synthetic fibers in agricultural mulching films, packaging plastic products, washing machine wastewater and the like.
The micro-plastics are difficult to degrade, can exist in the environment for a long time, and are transmitted to various ecosystems through wind power, water flow, food chains and the like, so that the damage to the environment and organisms is caused. Specifically, the micro-plastics can influence digestion and absorption of organisms, release organic monomers and toxic additives, cause combined pollution by adsorption, interfere endocrine functions of the organisms, induce genetic aberration and harm reproduction and development. In addition, the microplastic can also change the physicochemical properties of soil, influence soil animals and microbial communities, and reduce the quality and fertility of the soil. However, under certain conditions, microplastics may also have a positive effect on soil carbon sequestration.
At present, no related research or report exists on how to combine biological bacterial fertilizer with microplastic to improve the carbon fixing capacity of soil.
In view of this, the present invention has been made.
Disclosure of Invention
The invention aims to provide a biological bacterial fertilizer and application thereof in combination with microplastic to improve carbon sequestration capacity of soil.
The invention is realized in the following way:
in one aspect, the invention provides a biological bacterial fertilizer, which comprises the following raw materials of microbial agent and organic carrier;
the bacterial strain in the microbial agent is selected from bacillus belicus, bacillus methylotrophicus and bacillus licheniformis, and the ratio of the viable count of bacillus belicus, bacillus methylotrophicus and bacillus licheniformis in the microbial agent is as follows: 1 to 3: 5-10: 5 to 10; the organic matter carrier comprises a honey fermentation liquid and animal waste fermentation products, and the mass ratio of the honey fermentation liquid to the animal waste fermentation products in the organic matter carrier is 10-40: 60 to 90 percent.
In some embodiments, the preparation steps of the microbial agent include the following steps:
s1, performing aerobic culture on bacillus belicus, bacillus methylotrophicus and bacillus licheniformis for 24-96 hours at the temperature of 25-35 ℃ and the pH value of 6.5-7.5;
s2, after activation and expansion culture, coupling the microbial agent with an embedding material prepared from sodium alginate and calcium chloride, and preparing the microbial agent microcapsule by spray drying.
In some embodiments, the total viable count in the biological bacterial fertilizer is more than or equal to 2.0X10 8 cfu/g。
In some embodiments, the bacillus belicus has a preservation number of cgmccno.20317.
In some embodiments, the bacillus methylotrophicus has a preservation number of cctcm 2013462.
In some embodiments, the bacillus licheniformis has a preservation number of CGMCC No.24738.
In some embodiments, the raw materials of the biological bacterial fertilizer further comprise a regulator, a nitrogenous fertilizer, a phosphate fertilizer, a potash fertilizer, a calcium fertilizer, a magnesium fertilizer and a trace element fertilizer.
In some embodiments, the biological bacterial fertilizer comprises the following raw materials in parts by weight: 1-10 parts of microbial agent; 30-70 parts of organic matter carrier; 0.5 to 5 parts of regulator; 1-10 parts of nitrogenous fertilizer; 1-15 parts of phosphate fertilizer; 1-10 parts of potash fertilizer; 1-10 parts of calcium fertilizer; 1-5 parts of magnesium fertilizer; 1-5 parts of trace element fertilizer.
In some embodiments, the modifier is a zeolite powder, phosphogypsum and quicklime composition, wherein the mass ratio of the zeolite powder, the phosphogypsum and the quicklime is 10-45: 5-30: 5 to 25.
In some embodiments, the preparation method of the biological bacterial fertilizer comprises the following steps:
s1, mixing the honey fermentation liquor and the animal manure fermentation product according to a mass ratio to prepare an organic matter carrier;
s2, uniformly mixing the microbial agent, the organic matter carrier and the regulator according to a proportion to obtain a mixture, covering the mixture with a film, punching holes on the film for ventilation, and then fermenting at 50-85 ℃ for 7-10 days;
s3, uncovering the film to fully stir the mixture again, curing for 10-15 days, and further evaporating the obtained mixture to remove water to obtain the biological bacterial fertilizer with the organic matter of more than or equal to 30%;
s4, adding the nitrogenous fertilizer, the phosphate fertilizer, the potash fertilizer, the calcium fertilizer, the magnesium fertilizer and the trace element fertilizer into the organic biological bacterial fertilizer according to a proportion, and uniformly mixing to obtain the biological bacterial fertilizer.
On the other hand, the invention also provides application of the biological bacterial fertilizer and the microplastic in combination in enhancing the carbon fixation capacity of soil.
In some embodiments, the microplastic comprises a biodegradable microplastic.
In some embodiments, the biodegradable microplastic comprises at least one of polybutylene adipate terephthalate (PBAT), polycaprolactone (PCL), polybutylene succinate (PBS), polybutylene succinate-adipate (PBSA), carbon dioxide copolymer (PPC), polylactic acid (PLA), polyhydroxyalkanoate (PHA), and starch plastic.
The invention has the following beneficial effects:
(1) The biological bacterial fertilizer adopts a mixture of a honey fermentation liquid and animal manure fermentation products as an organic carrier, and is matched with specific microorganism bacteria and organic and inorganic nutrition fertilizers to carry out fermentation treatment, so that the biological bacterial fertilizer containing more than or equal to 30% of organic matters is prepared; compared with the traditional organic fertilizer, the bacterial fertilizer has the advantages of complete nutrients, low cost, stable quality, high fertilizer efficiency and complete functions, and all the contained components are environment-friendly products, so that the bacterial fertilizer accords with the concept of sustainable development;
(2) The invention provides a method for improving the carbon fixing capability of soil by combining an organic biological bacterial fertilizer with micro-plastics, which has the characteristics of environmental protection, sustainability and high efficiency; the method can effectively improve the pH value, the SOC and the OM of the soil, thereby generating better comprehensive benefit.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.
Soil, while an important source of greenhouse gases for carbon dioxide and methane production, can simultaneously sequester carbon as a carbon sink through soil organic matter. Therefore, reducing greenhouse gas emissions from the soil and increasing carbon sequestration in the soil are significant for mitigating climate change. In order to improve the carbon fixing capability of soil, the invention provides a biological bacterial fertilizer, which comprises the following raw materials of microbial agent, organic matter carrier, regulator, nitrogen fertilizer, phosphate fertilizer, potash fertilizer, calcium fertilizer, magnesium fertilizer and trace element fertilizer.
Specifically, the biological bacterial fertilizer comprises the following raw materials in parts by weight: 1-10 parts of microbial agent; 30-70 parts of organic matter carrier; 0.5 to 5 parts of regulator; 1-10 parts of nitrogenous fertilizer; 1-15 parts of phosphate fertilizer; 1-10 parts of potash fertilizer; 1-10 parts of calcium fertilizer; 1-5 parts of magnesium fertilizer; 1-5 parts of trace element fertilizer.
In the invention, the total viable count in the microbial agent is more than or equal to 2.0x10 8 cfu/g。
Wherein the strain in the microbial agent is selected from bacillus belicus, bacillus methylotrophicus and bacillus licheniformis. The ratio of the viable count of bacillus belicus, bacillus methylotrophicus and bacillus licheniformis in the microbial agent is as follows: 1 to 3: 5-10: 5 to 10.
Preferably, the preservation number of bacillus belicus is CGMCC NO.20317; the preservation number of the bacillus methylotrophicus is CCTCCM2013462; the preservation number of the bacillus licheniformis is CGMCC NO.24738. The above bacillus belicus, bacillus methylotrophicus and bacillus licheniformis are all existing strains and have been disclosed in patents cn202110072888.x, CN201410148228.5 and CN202210864870.8, respectively.
By adopting the technical scheme, the addition of the microbial agent can improve soil fertility and promote crops to absorb nutrition, and meanwhile, microorganisms in the composite microbial agent can also release auxin, such as indoleacetic acid and the like, in the soil so as to regulate and control the growth of crops.
The bacillus bailii can prevent and treat plant diseases, is soil-promoting bacteria, secretes bacteriostatic active metabolites to inhibit the growth of pathogenic bacteria, and induces a plant defense system to resist the invasion of the pathogenic bacteria so as to realize biological prevention and treatment; meanwhile, bacillus bailii can secrete various plant hormones and volatile compounds such as IAA, NH and ACC deaminase, so that plant growth can be promoted, soil nutrients can be increased, soil structure can be improved, and fertilizer utilization rate can be improved.
The antifungal active lipopeptide compound produced by the methylotrophic bacillus has stronger antibacterial activity on plant fungus diseases, has obvious control effects on cucumber, tomato gray mold, late blight, cotton yellow wilt, apple tree rot and the like, and can also prevent and treat citrus canker, cucumber bacterial angular leaf spot, rice bacterial leaf spot and the like; meanwhile, bacillus methylotrophicus produces aminopeptidase substances and simultaneously serves as PGPR (plant rhizosphere growth promoting bacteria) for promoting plant growth.
The bacillus licheniformis can generate endophytic spores, has strong heat resistance, high growth speed and simple nutrition requirement, is easy to survive, colonize and reproduce on the surface of a plant, has strong competition and colonization capacity, thereby occupying the infection sites of pathogenic bacteria, consuming the surrounding nutrients, preventing and interfering the infection of the pathogenic bacteria to the leaf surfaces and other organs of the plant, and playing a role in preventing and inhibiting bacteria; in addition, the bacillus licheniformis not only can inhibit plant pathogenic bacteria, but also can induce a disease resistance mechanism of plants so as to enhance the disease resistance of the plants.
The bacillus beliae, the bacillus methylotrophicus and the bacillus licheniformis are selected, and the bacillus beliae and the bacillus methylotrophicus have similar functional effects and functional superposition effects. The bacillus licheniformis can be well adapted to alkaline environment, three strains of bacteria are mutually matched, the bacillus licheniformis can be adapted to more complex environment, and the effect of the microbial agent is enhanced.
The organic matter carrier comprises honey fermentation liquid and animal feces fermentation product. Specifically, the organic matter carrier honey fermentation liquid is a fermentation product of livestock manure or zoo animal manure in a farm.
Preferably, the mass ratio of the honey fermentation liquid to the animal manure fermentation product is 10-40: 60 to 90 percent.
By adopting the technical scheme, the waste is effectively utilized, so that the cost is low, the emission of the waste can be reduced, and the environment and the effective utilization of resources are protected.
Meanwhile, in order to provide sufficient nutrients for crops, the biological bacterial fertilizer also comprises a nitrogenous fertilizer, a phosphate fertilizer, a potash fertilizer, a calcium fertilizer, a magnesium fertilizer and a trace element fertilizer.
As for the kind of nitrogen fertilizer, ammonium nitrogen fertilizer, nitrate nitrogen fertilizer, urea nitrogen fertilizer or other kinds of nitrogen fertilizer can be mentioned; the phosphate fertilizer can be monoammonium phosphate, diammonium phosphate, ammonium polyphosphate, nitrophosphate, double-burned phosphate fertilizer, calcium magnesium phosphate fertilizer, triple superphosphate, calcium hydrogen phosphate, phosphate rock or other kinds of phosphate fertilizer; the potash fertilizer can be potassium nitrate, monopotassium phosphate, potassium sulfate, potassium chloride or other kinds of potash fertilizers; the calcium fertilizer can be quicklime, slaked lime, lime powder, gypsum, carbonic lime, calcium nitrate, calcium chloride or other kinds of calcium fertilizers; the magnesium fertilizer can be magnesium sulfate, magnesium nitrate, magnesium chloride, calcium magnesium phosphate, magnesite or other magnesium fertilizers; the trace element fertilizer can be molybdenum fertilizer, boron fertilizer, manganese fertilizer, zinc fertilizer, copper fertilizer, iron fertilizer or other kinds of trace element fertilizer. The specific choice of which nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, calcium fertilizer, magnesium fertilizer and trace element fertilizer can be selected according to actual needs, and the invention is not limited to this.
The specific microbial strain is prepared into a microbial agent, and the microbial agent is compounded with an organic matter carrier, a regulator, a nitrogenous fertilizer, a phosphate fertilizer, a potash fertilizer, a calcium fertilizer, a magnesium fertilizer, a trace element fertilizer and the like to obtain the biological bacterial fertilizer, so that the pH of soil, the organic carbon (SOC) of the soil and the Organic Matter (OM) of the soil can be improved, and further the growth of plants is promoted.
Specifically, the regulator is a composition of zeolite powder, phosphogypsum and quicklime, wherein the mass ratio of the zeolite powder to the phosphogypsum to the quicklime is 10-45: 5-30: 5 to 25. The regulator has unique acid-base regulation, adsorptivity, catalysis, biological activity and toxicity resistance.
As a general technical conception, the invention also provides a preparation method of the biological bacterial fertilizer, which comprises the following specific steps:
(1) Preparation of microbial agent:
performing aerobic culture on the selected bacillus belicus, bacillus methylotrophicus and bacillus licheniformis, performing activation, expanding culture and fermentation, coupling the bacillus belicus, the bacillus methylotrophicus and the bacillus licheniformis with an embedding material prepared from sodium alginate and calcium chloride, and preparing a composite microbial agent microcapsule by spray drying for later use;
wherein, the culture conditions of the aerobic culture are as follows: culturing for 24-96h at 25-35 ℃ under the condition that pH=6.5-7.5;
(2) Preparation of organic matter carrier:
mixing the honey fermentation liquor with livestock manure or zoo animal manure fermentation products in proportion for standby;
(3) Uniformly mixing the microbial agents prepared in the step (1) and the step (2) with an organic carrier according to a proportion, covering the mixture with a film after adding other regulators, punching and ventilating the film, carrying out high-temperature fermentation, curing the mixture for 10-15 days after uniformly mixing again, further evaporating the obtained mixture to remove water to obtain an organic biological bacterial fertilizer with the organic matter of more than or equal to 30%, and then adding nitrogen fertilizer, phosphate fertilizer, potash fertilizer, calcium fertilizer, magnesium fertilizer and trace element fertilizer into the organic biological bacterial fertilizer according to a proportion, and uniformly mixing to obtain the biological bacterial fertilizer;
wherein the high-temperature fermentation temperature is 50-85 ℃, and the fermentation time is 7-10 days.
Experiments prove that the biological bacterial fertilizer and the microplastic can have obvious influence on the physicochemical properties of soil after being combined: the pH, SOC and OM of the soil are improved, wherein the content of the SOC and the OM is obviously improved compared with that of the biological bacterial fertilizer which is singly applied, and the combination of the biological bacterial fertilizer and the microplastic is proved to have a synergistic effect.
The microplastic is at least one selected from polybutylene terephthalate (PBAT), polycaprolactone (PCL), polybutylene succinate (PBS), polybutylene succinate-adipic acid ester (PBSA), carbon dioxide copolymer (PPC), polylactic acid (PLA), polyhydroxyalkanoate (PHA) and starch plastic.
The features and capabilities of the present invention are described in further detail below in connection with the examples.
The preservation number of bacillus belicus adopted in the embodiment of the invention is CGMCC NO.20317; the preservation number of the bacillus methylotrophicus is CCTCCM2013462; the preservation number of the bacillus licheniformis is CGMCC NO.24738. Degradable plastic polybutylene adipate terephthalate (PBAT) was purchased from polyester limited, mountain and river, singkiang.
The methods used in the examples described below are conventional methods unless otherwise specified.
Example 1
The embodiment provides a biological bacterial fertilizer, which comprises the following raw materials:
60 parts of organic matter carrier; 10 parts of microbial agent; 5 parts of a regulator; 6 parts of nitrogenous fertilizer; 5 parts of phosphate fertilizer; 6 parts of potash fertilizer; 3 parts of calcium fertilizer; 3 parts of magnesium fertilizer; 2 parts of trace element fertilizer.
The organic matter carrier comprises a honey fermentation liquid and livestock manure or zoo animal manure fermentation products, and the mass ratio of the honey fermentation liquid to the livestock manure or zoo animal manure fermentation products is 1:4.
The regulator is a composition of zeolite powder, phosphogypsum and quicklime, wherein the mass ratio of the zeolite powder to the phosphogypsum to the quicklime is 45:30:25.
The viable count of the biological bacterial fertilizer is as follows: 2 billions/gram. Wherein, the ratio of the viable count of bacillus bailii, bacillus methylotrophicus and bacillus licheniformis is as follows: 3:10:10.
the biological bacterial fertilizer is prepared by the following steps:
s1, performing aerobic culture on selected bacillus belicus, bacillus methylotrophicus and bacillus licheniformis for 60 hours at the temperature of 28 ℃ and the condition of pH=6.8, performing activation, expansion culture and fermentation, coupling the bacillus belicus, the bacillus methylotrophicus and the bacillus licheniformis with embedding materials prepared from sodium alginate and calcium chloride, and preparing a composite microbial agent microcapsule for later use through spray drying;
s2, mixing the honey fermentation liquor with livestock manure or zoo animal manure fermentation products in proportion for later use;
s3, uniformly mixing the biological bacterial agents prepared in the steps S1 and S2 with an organic carrier and a regulator according to a proportion to obtain a mixture, covering the mixture with a film, punching and ventilating the film, performing high-temperature fermentation at 65 ℃ for 7 days, uniformly mixing the mixture again, aging the mixture for 14 days to obtain an organic biological bacterial fertilizer, and then adding a nitrogenous fertilizer, a phosphate fertilizer, a potash fertilizer, a calcium fertilizer, a magnesium fertilizer and a trace element fertilizer into the organic biological bacterial fertilizer according to a proportion, and uniformly mixing the organic biological bacterial fertilizer to obtain the biological bacterial fertilizer.
Example 2
The embodiment provides a biological bacterial fertilizer, and the specific preparation method is the same as that of embodiment 1, except that the biological bacterial fertilizer has different raw material compositions, and the specific raw material compositions are as follows:
65 parts of organic matter carrier; 5 parts of microbial agent; 5 parts of a regulator; 6 parts of nitrogenous fertilizer; 5 parts of phosphate fertilizer; 6 parts of potash fertilizer; 3 parts of calcium fertilizer; 3 parts of magnesium fertilizer; 2 parts of trace element fertilizer.
Example 3
The embodiment provides a biological bacterial fertilizer, and the specific preparation method is the same as that of embodiment 1, except that the biological bacterial fertilizer has different raw material compositions, and the specific raw material compositions are as follows:
70 parts of organic matter carrier; 10 parts of microbial agent; 5 parts of a regulator; 4 parts of nitrogen fertilizer; 4 parts of phosphate fertilizer; 4 parts of potash fertilizer; 3 parts of calcium fertilizer; 3 parts of magnesium fertilizer; 2 parts of trace element fertilizer.
Example 4
The embodiment provides a biological bacterial fertilizer, and the specific preparation method is the same as that of the embodiment 1, wherein the difference is that the ratio of the viable count of bacillus belicus, bacillus methylotrophicus and bacillus licheniformis in the microbial agent is different, and the ratio in the embodiment is as follows: 3:5:5.
example 5
The embodiment provides a biological bacterial fertilizer, and the specific preparation method is the same as that of the embodiment 1, wherein the difference is that the ratio of the viable count of bacillus belicus, bacillus methylotrophicus and bacillus licheniformis in the microbial agent is different, and the ratio in the embodiment is as follows: 1:10:10.
comparative example 1
The difference from example 1 is that no microbial agent is contained.
Comparative example 2
The difference from example 1 is that no organic carrier is contained.
Comparative example 3
The difference from example 1 is that the organic matter carrier and the microbial agent are not contained.
Comparative example 4
The difference from example 1 is that no regulator is contained.
Experimental example 1
The fertilizer efficiency test was performed on examples 1 to 5 and comparative examples 1 to 4, specifically as follows:
the biological bacterial fertilizers of the examples 1-5 and the comparative examples 1-4 are added into soil according to 0.5% w/w and uniformly mixed, the test crop is Shanghai green, the test adopts a pot culture mode, the specification is 230mm multiplied by 180mm, each pot is 6kg of soil, and each group is repeated for 6 pots; the Shanghai green seeds are sterilized twice by 75% ethanol for 2 minutes each time, then washed in sterile distilled water for 2 minutes, four-leaf and one-heart period 3 seedlings with uniform growth vigor and strong root systems are selected for transplanting after germination, fertilization is carried out according to a fertilization scheme, and basic indexes such as chlorophyll, plant height, total plant dry weight and the like among groups after 28 days are measured. The specific effects are shown in table 1 below. A blank group, which refers to the appearance of Shanghai green without fertilizer applied, was also added in table 1.
TABLE 1 contrast data for chlorophyll, plant height, whole plant dry weight, yield increase of Shanghai green crops under different treatments
| Treatment of | Chlorophyll (SPAD value) | Plant height/cm | Dry weight/g of whole plant | Yield increase% |
| Blank space | 35.2 d | 22.3 d | 7.80 d | - |
| Example 1 | 39.2 a | 27.1 a | 9.90 a | 26.9 |
| Example 2 | 38.7 b | 26.6 a | 9.60 a | 23.1 |
| Example 3 | 38.5 b | 26.8 a | 9.72 a | 24.6 |
| Example 4 | 38.6 b | 26.9 a | 9.60 a | 23.1 |
| Example 5 | 38.9 b | 26.9 a | 9.78 a | 25.4 |
| Comparative example 1 | 38.3 b | 26.2 b | 9.18 b | 17.7 |
| Comparative example 2 | 37.7 c | 25.7 c | 8.82 c | 13.1 |
| Comparative example 3 | 37.3 c | 25.4 c | 8.58 c | 10.0 |
| Comparative example 4 | 38.3 b | 26.1 b | 8.94 b | 14.6 |
Note that: different lower case letters in the same line indicate that there is a significant difference between the different treatments (P < 0.05).
As can be seen from Table 1, the Shanghai green to which the biological bacterial manure of examples 1 to 5 was applied showed a significant increase in chlorophyll, plant height and total plant dry weight as compared to Shanghai green to which no biological bacterial manure was applied, and an increase in chlorophyll, plant height and total plant dry weight as compared to comparative examples 1 to 4, which indicates that the biological bacterial manure of examples 1 to 5 had good fertilizer efficiency, with the best fertilizer efficiency of example 1 being the optimal formulation.
Experimental example 2
The biological bacterial fertilizer and the microplastic of the example 1 are combined for verifying the soil carbon fixation effect, and the steps are as follows:
1. the soil tested was selected from the farmland soil in the Ming's area, where the plastic product was never used. The experiment adopts a potting culture mode, the specification is 230mm multiplied by 180mm, each pot is 6kg of soil (according to actual conditions), and basic physicochemical indexes (pH, organic matters and the like) of the soil are confirmed; the method comprises the following steps: BF (0.5% w/w) and PBAT-MPs (0.1% w/w), BF (0.5% w/w) and PBAT-MPs (0.5% w/w), BF (0.5% w/w) and PBAT-MPs (2.5% w/w); BF (0.5% w/w) was applied only; CF (0.5% w/w) and PBAT-MPs (0.1% w/w), CF (0.5% w/w) and PBAT-MPs (0.5% w/w), CF (0.5% w/w) and PBAT-MPs (2.5% w/w); CF (0.5% w/w) alone; repeat 6 pots per group; wherein BF is the biological bacterial fertilizer corresponding to the example 1, CF is the fertilizer corresponding to the comparative example 1 without adding microbial inoculum, and CK is a blank group;
2. tested crops: shanghai green, a common commercial crop, has a growth period of about 20-30 days; sterilizing Shanghai green seeds twice with 75% ethanol for 2 min each time, washing in sterile distilled water for 2 min, and transplanting after germination by selecting four-leaf and one-heart 3-plant seedlings with uniform growth vigor and strong root systems;
3. the microplastic is degradable plastic poly (butylene adipate-terephthalate) (PBAT);
4. microplastic treatment: crushing degradable plastic polybutylene adipate terephthalate (PBAT) microplastic in a crusher, and sieving the crushed microplastic with a 100-mesh sieve to obtain microplastic powder;
5. culture period: the culture period is 28 days, in the process, a weighing method is used for maintaining the soil moisture content to be 60% -80% of the maximum water holding capacity of the soil field, and the soil property and pH value in the culture process are observed;
6. and (3) measuring physical and chemical indexes of soil: the change of indexes such as pH, SOC, organic matters and the like of the soil is measured according to a planting period of 28 days as an example. And detecting the related indexes by using a related national standard method.
TABLE 2 influence of treatment groups on the physicochemical index of Shanghai Green root System soil
Note that: the different lower case letters of the same column indicate that there was a significant difference between the different treatments (P < 0.05).
As can be seen from table 2, the application of the biological bacterial fertilizer and the addition of the PBAT-MPs have obvious effects on the physicochemical properties of the soil, the application of the biological bacterial fertilizer increases the pH (13.32%), SOC (70.81%), OM (71.18%), and the addition of the PBAT-MPs further increases the pH of the soil, and it is noted that both the SOC and OM content are significantly promoted as the proportion of the MPs increases, showing a synergistic effect with the action effect of the biological bacterial fertilizer. Furthermore, the addition of 2.5% w/w PBAT-MPs increased the SOC content by a factor of 2.63 and the OM content by a factor of 2.68, respectively, relative to the biological bacterial fertilizer alone. This may be related to the following reasons: (1) The micro plastic can be used as a potential matrix or carrier to increase the adsorption quantity of organic matters in soil; (2) The microplastic can change the soil structure and the porosity, and increase the protection space of organic matters in the soil. And the fertilizer pH, SOC, OM added with the microbial agent in the comparative example 1 is low in carbon fixing capability and weak in carbon fixing capability, so that the microbial agent and the micro plastic have synergistic effect on improving the carbon fixing capability of soil.
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. The biological bacterial fertilizer is characterized by comprising the following raw materials of microbial agents and organic matter carriers;
the strain in the microbial agent is selected from bacillus belicus, bacillus methylotrophicus and bacillus licheniformis;
the ratio of the viable count of bacillus belicus, bacillus methylotrophicus and bacillus licheniformis in the microbial agent is as follows: 1 to 3: 5-10: 5 to 10;
the organic matter carrier comprises a honey fermentation liquid and an animal waste fermentation product, and the mass ratio of the honey fermentation liquid to the animal waste fermentation product in the organic matter carrier is 10-40: 60 to 90 percent.
2. The biological bacterial fertilizer according to claim 1, wherein the preparation step of the microbial agent comprises the steps of:
s1, performing aerobic culture on bacillus belicus, bacillus methylotrophicus and bacillus licheniformis for 24-96 hours at the temperature of 25-35 ℃ and the pH value of 6.5-7.5;
s2, after activation and expansion culture, coupling the microbial agent with an embedding material prepared from sodium alginate and calcium chloride, and preparing a composite microbial agent microcapsule by spray drying, namely the microbial agent.
3. The biological bacterial fertilizer according to claim 2, wherein the total viable count in the biological bacterial fertilizer is not less than 2.0x10 8 cfu/g。
4. The biological bacterial fertilizer according to claim 3, wherein the bacillus besseyi has a preservation number of CGMCC No.20317;
preferably, the preservation number of the bacillus methylotrophicus is CCTCCM2013462;
preferably, the preservation number of the bacillus licheniformis is CGMCC NO.24738.
5. The biological bacterial fertilizer according to claim 4, wherein the raw materials of the biological bacterial fertilizer further comprise a regulator, a nitrogen fertilizer, a phosphate fertilizer, a potash fertilizer, a calcium fertilizer, a magnesium fertilizer and a trace element fertilizer.
6. The biological bacterial fertilizer according to claim 5, wherein the raw materials of the biological bacterial fertilizer comprise, in parts by weight: 1-10 parts of microbial agent; 30-70 parts of organic matter carrier; 0.5 to 5 parts of regulator; 1-10 parts of nitrogenous fertilizer; 1-15 parts of phosphate fertilizer; 1-10 parts of potash fertilizer; 1-10 parts of calcium fertilizer; 1-5 parts of magnesium fertilizer; 1-5 parts of trace element fertilizer.
7. The biological bacterial fertilizer according to claim 6, wherein the regulator is a composition of zeolite powder, phosphogypsum and quicklime, wherein the mass ratio of the zeolite powder, the phosphogypsum and the quicklime is 10-45: 5-30: 5 to 25.
8. The biological bacterial fertilizer according to claim 7, wherein the preparation method of the biological bacterial fertilizer comprises the following steps:
s1, mixing the honey fermentation liquor and the animal manure fermentation product according to a mass ratio to prepare an organic matter carrier;
s2, uniformly mixing the microbial agent, the organic matter carrier and the regulator according to a proportion to obtain a mixture, covering the mixture with a film, punching holes on the film for ventilation, and then fermenting at 50-85 ℃ for 7-10 days;
s3, uncovering the film to fully stir the mixture again, curing for 10-15 days, and further evaporating the obtained mixture to remove water to obtain the biological bacterial fertilizer with the organic matter of more than or equal to 30%;
s4, adding the nitrogenous fertilizer, the phosphate fertilizer, the potash fertilizer, the calcium fertilizer, the magnesium fertilizer and the trace element fertilizer into the organic biological bacterial fertilizer according to a proportion, and uniformly mixing to obtain the biological bacterial fertilizer.
9. Use of a biological bacterial fertilizer according to any one of claims 1-8 in combination with a microplastic for enhancing the carbon sequestration capacity of soil.
10. The use according to claim 9, wherein the microplastic comprises a biodegradable microplastic;
preferably, the biodegradable microplastic comprises at least one of polybutylene adipate terephthalate (PBAT), polycaprolactone (PCL), polybutylene succinate (PBS), polybutylene succinate-adipate (PBSA), carbon dioxide copolymer (PPC), polylactic acid (PLA), polyhydroxyalkanoate (PHA) and starch plastic.
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| CN115960744A (en) * | 2022-07-21 | 2023-04-14 | 北京世纪阿姆斯生物工程有限公司 | Bacillus licheniformis and application thereof |
| CN115960744B (en) * | 2022-07-21 | 2024-09-06 | 北京世纪阿姆斯生物工程有限公司 | Bacillus licheniformis and application thereof |
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