CN116806926A - Preparation method of low-protein daily ration for high-yield cows - Google Patents

Preparation method of low-protein daily ration for high-yield cows Download PDF

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CN116806926A
CN116806926A CN202310892976.3A CN202310892976A CN116806926A CN 116806926 A CN116806926 A CN 116806926A CN 202310892976 A CN202310892976 A CN 202310892976A CN 116806926 A CN116806926 A CN 116806926A
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parts
low
protein
yield
cows
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李宏伟
白启扬
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Ningxia Guangdeyuan Agriculture And Animal Husbandry Development Co ltd
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Ningxia Guangdeyuan Agriculture And Animal Husbandry Development Co ltd
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Abstract

The application relates to the technical field of livestock and poultry feed preparation, and discloses a preparation method of a low-protein ration for high-yield cows, which comprises the following raw materials in parts by mass: 10-20 parts of high-quality alfalfa, 7-26 parts of whole plant corn silage, 4-19 parts of corn gluten meal, 13-18 parts of soybean meal, 3-8 parts of whole cotton seeds, 4-16 parts of rumen bypass fatty acid calcium, 12-17 parts of potassium bicarbonate, 6-17 parts of rumen lysine, 6-8 parts of methionine, 2-9 parts of high-yield cow premix, 18-22 parts of domestic alfalfa, 16-27 parts of wheat straw, 14-24 parts of millet grass, 15-22 parts of oat grass and 12-16 parts of imported alfalfa. The liquid fermentation product of natural low protease is matched with the basic mixed raw materials, so that the protein content in the raw materials is reduced, the intake of other nutrient substances is not influenced, and the base liquid is added to protect animal liver organs, reduce excrement smell, optimize the feed formula, reduce the production cost and reduce environmental pollution.

Description

Preparation method of low-protein daily ration for high-yield cows
Technical Field
The application relates to the technical field of livestock and poultry feed preparation, in particular to a preparation method of a low-protein daily ration for high-yield cows.
Background
The protein feed is characterized in that the natural water content is lower than 45%, the crude fiber in the dry matter is lower than 18%, and the crude protein content in the dry matter reaches or exceeds 20% of nutrient substances such as beans, cakes, fish meal and the like, and the protein feed is divided into four categories of vegetable protein feed, animal protein feed, single-cell protein feed and non-protein nitrogen feed, and almost all industrial protein feeds are mature seeds and processed products of the seeds, and the nitrogen-containing compounds are mainly proteins, so that the proteins are very important for maintaining the growth and development of livestock and poultry, improving the reproductive performance and the production performance of cows and the like.
The nutrition required by cows in the current large-scale cow breeding base is very high, especially protein nutrition, but the result of large amount of protein intake is that the protein in the cow body is increased, so that the cow body can squeeze other organs to run, especially important organs such as mess, and the intake of high protein feed, the excrement and urine excreted by the cows are very high in nitrogen content, and meanwhile, nitrogen substances such as ammonia gas, nitrous oxide and nitrate are generated when the cow cannot be effectively utilized, so that the environment pollution of a breeding area is caused, the subsequent maintenance cost is high and the economic benefit of cow generation is not proportional.
Disclosure of Invention
Aiming at the defects of the prior art, the application provides a preparation method of low-protein daily ration for high-yield cows, which solves the problems that organs are damaged to influence the health of cows caused by taking a large amount of protein nutrient substances in the conventional cow cultivation, and excrement and urine excreted after taking are very high in nitrogen content and cannot be utilized, pollute the environment of a cultivation area and increase the subsequent maintenance cost.
In order to achieve the above purpose, the application is realized by the following technical scheme: the low-protein daily ration for the high-yield dairy cows comprises the following raw materials in parts by weight: 10 to 20 parts of high-quality alfalfa, 7 to 26 parts of whole plant corn silage, 4 to 19 parts of corn gluten meal, 13 to 18 parts of soybean meal, 3 to 8 parts of whole cotton seeds, 4 to 16 parts of rumen bypass fatty acid calcium, 12 to 17 parts of potassium bicarbonate, 6 to 17 parts of rumen lysine, 6 to 8 parts of methionine, 2 to 9 parts of high-yield cow premix, 18 to 22 parts of domestic alfalfa, 16 to 27 parts of wheat straw, 14 to 24 parts of millet grass, 15 to 22 parts of oat grass, 12 to 16 parts of imported alfalfa, 15 to 18 parts of alfalfa silage, 6 to 9 parts of water, 7 to 13 parts of pressed corn, 7 to 9 parts of brewer's grains, 3 to 8 parts of corn meal, 5 to 16 parts of cotton meal, 7 to 15 parts of beet particles, 6 to 14 parts of puffed soybean, 2 to 4 parts of urea 2-9 parts of cow premix, 6-15 parts of dry cow premix, 7-12 parts of cow premix, 8-15 parts of cow premix, 5-7 parts of rumen bypass choline, 10-15 parts of fat powder, 1-6 parts of yeast, 3-4 parts of baking soda, 3-15 parts of soybean hulls, 6-12 parts of corn steep liquor, 1-8 parts of corn steep liquor, 2-4 parts of glucose, 1-3 parts of peptide An Jian, 3-6 parts of mildew remover, 5-7 parts of muskmelon, 4-6 parts of muskmelon, 4-15 parts of lactobacillus, 3-6 parts of amino acid, 6-11 parts of silybum marianum mixed solution, 3-8 parts of compound enzyme, 3-8 parts of soybean molasses, 3-8 parts of orange peel water, 4-5 parts of soda and 2-8 parts of deodorizing solution.
Preferably, the deodorizing solution is prepared from urea and humic acid according to the adding ratio of 0.5:1.2, mixing and preparing.
Preferably, the silybum marianum mixed solution is prepared by mixing and distilling silybum marianum meal, myristic acid, aqueous emulsion, honeysuckle flower and glucose drops.
The preparation method of the low-protein daily ration for the high-yield cows adopts the low-protein daily ration for the high-yield cows, and comprises the following steps:
s1, precisely weighing high-quality prandial, whole plant corn silage, corn protein powder, soybean meal, whole cotton seed, rumen bypass fatty acid calcium, potassium bicarbonate, rumen lysine, methionine, high-yield cow premix, domestic alfalfa, wheat straw, millet grass, oat grass, imported alfalfa grass, alfalfa silage, water, pressed corn, brewer' S grains, corn flour, cotton meal, beet particles, puffed soybean, urea, cow premix, dry cow premix, rumen bypass choline, fat powder, yeast, sodium bicarbonate, soybean hulls, immediately absorbable soybean meal with tumor bypass flavor, glucose and peptide An Jian according to the formula parts, mixing in a stirring device, simultaneously adding a mold removing agent for mixing, and drying to obtain a basic mixed raw material;
s2, respectively diluting the amino acid and the microbial agent with distilled water according to a ratio of 1:4, placing the obtained mixed solution into a temperature of 40-45 ℃ for intermittent stirring for 2-4 hours to obtain a bacterial solution, adding lactobacillus to carry out fluid replacement, obtaining a bacterial replacement solution, then taking melon and muskmelon to crush and squeeze juice for mixing, adding a thickening agent to obtain melon slurry, finally adding the obtained base supplementing solution into the melon slurry according to 12%, carrying out anaerobic fermentation at a constant temperature of 40-45 ℃ for 72 hours, and obtaining the liquid fermentation product containing natural low protease.
S3, filtering, concentrating, spraying and drying the obtained liquid fermentation product at low temperature, and then adding the liquid fermentation product into the basic mixed raw material for uniform mixing to obtain the low-protein ration carrier raw material;
s4, precisely weighing the silybum marianum mixed solution, synchronously placing the silybum marianum mixed solution, the soy molasses and the compound enzyme into a reaction kettle, and uniformly mixing the silybum marianum mixed solution with ultrasonic waves to obtain a mixture A;
s5, mixing orange peel water and soda water, taking a proper amount of distilled water, placing the distilled water into another reaction kettle, adding precisely weighed deodorant solution, and fully dissolving the deodorant solution by ultrasonic waves to obtain a ingredient B;
s6, pouring the ingredient A into the ingredient B, stirring while pouring, shearing at a high speed, and homogenizing in a high-pressure homogenizer to obtain the additive base solution.
And S7, adding the base liquid in a drop spraying mode into the obtained low-protein daily ration carrier raw material, fully stirring and subsequently drying, and preparing the obtained raw material by a feed machine to obtain the low-protein daily ration for the high-yield dairy cows.
Preferably, in the step S1, the basic mixed raw material is sent into a dryer for drying treatment, the drying temperature is 60-70 ℃, and the water content of the basic mixed raw material is stabilized at 30% -35%.
Preferably, in the step S2, the thickener is any one of carrageenan, xanthan gum, guar gum, konjac gum, locust bean gum, edible gelatin and alginate.
Preferably, in the step S4, the complex enzyme includes any one of phytase, protease, amylase, xylanase and coenzyme.
Preferably, in the step S6, the mixing ratio of the ingredient a to the ingredient B is 3:0.8.
Preferably, in the step S6, the high-speed shearing rate is 18000-19000 r/min, and the shearing time is 2-4 min; the homogenizing pressure of high-pressure homogenizing is 70-140 MPa, and the homogenizing times are 6-12 times.
Preferably, the high-yield dairy cow low-protein daily ration is applied to the dairy cow farm for accurate nutrition and carbon nitrogen emission reduction.
The application provides a preparation method of a low-protein daily ration for high-yield cows. The beneficial effects are as follows:
1. according to the application, the basic mixed raw materials mixed by the raw materials are matched with the liquid fermentation product of the natural low protease, so that the protein content in the raw materials is reduced, the normal intake of other nutrients is not influenced, and the silybum marianum mixed liquid and the deodorizing solution in the base liquid are added to protect animal liver organs on one hand, and the excreted fecal odor is reduced on the other hand, so that the feed formula is optimized, the protein intake frequency in the formula is relieved, the cultivation cost is reduced, and the environmental pollution is reduced.
2. According to the application, the nutritional requirements of the high-yield cows are met, the feeding management is optimized, the utilization efficiency of carbon and nitrogen in the daily ration is improved, the greenhouse gas emission in the milk production process is reduced, the production efficiency is improved, the milk cost is reduced, the regulation measures of nutrition, management, fecal sewage treatment and the like of the high-yield cows under the condition of low-protein daily ration are integrated, and an interaction network of accurate nutrition and carbon and nitrogen emission reduction of the cows is established, so that the method is convenient to popularize.
Drawings
FIG. 1 is a flow chart of accurate nutrition and carbon nitrogen emission reduction of a dairy farm according to the present application.
Detailed Description
The following description of the embodiments of the present application will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present application, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
The application provides a preparation method of a low-protein daily ration for high-yield cows.
According to one aspect of the application, the application provides a low-protein daily ration for high-yield cows, which comprises the following raw materials in parts by weight: 10 to 20 parts of high-quality alfalfa, 7 to 26 parts of whole plant corn silage, 4 to 19 parts of corn gluten meal, 13 to 18 parts of soybean meal, 3 to 8 parts of whole cotton seeds, 4 to 16 parts of rumen bypass fatty acid calcium, 12 to 17 parts of potassium bicarbonate, 6 to 17 parts of rumen lysine, 6 to 8 parts of methionine, 2 to 9 parts of high-yield cow premix, 18 to 22 parts of domestic alfalfa, 16 to 27 parts of wheat straw, 14 to 24 parts of millet grass, 15 to 22 parts of oat grass, 12 to 16 parts of imported alfalfa, 15 to 18 parts of alfalfa silage, 6 to 9 parts of water, 7 to 13 parts of pressed corn, 7 to 9 parts of brewer's grains, 3 to 8 parts of corn meal, 5 to 16 parts of cotton meal, 7 to 15 parts of beet particles, 6 to 14 parts of puffed soybean, 2 to 4 parts of urea 2-9 parts of cow premix, 6-15 parts of dry cow premix, 7-12 parts of cow premix, 8-15 parts of cow premix, 5-7 parts of rumen bypass choline, 10-15 parts of fat powder, 1-6 parts of yeast, 3-4 parts of baking soda, 3-15 parts of soybean hulls, 6-12 parts of corn steep liquor, 1-8 parts of corn steep liquor, 2-4 parts of glucose, 1-3 parts of peptide An Jian, 3-6 parts of mildew remover, 5-7 parts of muskmelon, 4-6 parts of muskmelon, 4-15 parts of lactobacillus, 3-6 parts of amino acid, 6-11 parts of silybum marianum mixed solution, 3-8 parts of compound enzyme, 3-8 parts of soybean molasses, 3-8 parts of orange peel water, 4-5 parts of soda and 2-8 parts of deodorizing solution.
Further, the deodorizing solution is prepared from urea and humic acid according to the adding ratio of 0.5:1.2, mixing and preparing.
Further, the silybum marianum mixed solution is prepared by mixing and distilling silybum marianum meal, myristic acid, aqueous emulsion, honeysuckle flower and glucose drops.
According to another aspect of the present application, an embodiment of the present application provides a method for preparing a low protein ration for a high-yield dairy cow, using a raw material provided by the low protein ration for the high-yield dairy cow, including the steps of:
s1, precisely weighing high-quality prandial, whole plant corn silage, corn protein powder, soybean meal, whole cotton seed, rumen bypass fatty acid calcium, potassium bicarbonate, rumen lysine, methionine, high-yield cow premix, domestic alfalfa, wheat straw, millet grass, oat grass, imported alfalfa grass, alfalfa silage, water, pressed corn, brewer' S grains, corn flour, cotton meal, beet particles, puffed soybean, urea, cow premix, dry cow premix, rumen bypass choline, fat powder, yeast, sodium bicarbonate, soybean hulls, immediately absorbable soybean meal with tumor bypass flavor, glucose and peptide An Jian according to the formula parts, mixing in a stirring device, simultaneously adding a mold removing agent for mixing, and drying to obtain a basic mixed raw material;
in the step S1, the basic mixed raw material is sent into a dryer for drying treatment, the drying temperature is 60-70 ℃, and the water content of the basic mixed raw material is stabilized at 30-35%.
S2, respectively diluting amino acid and a microbial agent with distilled water according to a ratio of 1:4, placing the obtained mixed solution into a temperature of 40-45 ℃ for intermittent stirring for 2-4 hours to obtain a bacterial solution, adding lactobacillus to carry out fluid replacement, obtaining a bacterial replacement solution, then taking melon and muskmelon to crush and squeeze juice for mixing, adding a thickening agent to obtain melon slurry, finally adding the obtained base supplementing solution into the melon slurry according to 12%, carrying out anaerobic fermentation at a constant temperature of 40-45 ℃ for 72 hours, and obtaining a liquid fermentation product containing natural low protease;
in the step S2, the thickening agent is any one of carrageenan, xanthan gum, guar gum, konjak gum, locust bean gum, edible gelatin and alginate.
S3, filtering, concentrating, spraying and drying the obtained liquid fermentation product at low temperature, and then adding the liquid fermentation product into the basic mixed raw material for uniform mixing to obtain the low-protein ration carrier raw material;
s4, precisely weighing the silybum marianum mixed solution, synchronously placing the silybum marianum mixed solution, the soy molasses and the compound enzyme into a reaction kettle, and uniformly mixing the silybum marianum mixed solution with ultrasonic waves to obtain a mixture A;
in the step S4, the complex enzyme is prepared by mixing any one of phytase, protease, amylase and xylanase with coenzyme.
S5, mixing orange peel water and soda water, taking a proper amount of distilled water, placing the distilled water into another reaction kettle, adding precisely weighed deodorant solution, and fully dissolving the deodorant solution by ultrasonic waves to obtain a ingredient B;
s6, pouring the ingredient A into the ingredient B, stirring while pouring, shearing at a high speed, and homogenizing in a high-pressure homogenizer to obtain an added base solution;
further, in the step S6, the mixing ratio of the ingredient A to the ingredient B is 3:0.8.
Further, in the step S6, the high-speed shearing rate is 18000-19000 r/min, and the shearing time is 2-4 min; the homogenizing pressure of high-pressure homogenizing is 70-140 MPa, and the homogenizing times are 6-12 times.
And S7, adding the base liquid in a drop spraying mode into the obtained low-protein daily ration carrier raw material, fully stirring and subsequently drying, and preparing the obtained raw material by a feed machine to obtain the low-protein daily ration for the high-yield dairy cows.
In order to describe the preparation method of the low protein ration for the high-yield dairy cows in detail, the following specific description is given by combining examples.
Feed example 1:
the embodiment of the application provides a low-protein daily ration for high-yield cows, which comprises the following raw materials in parts by mass: 10 parts of high-quality alfalfa, 7 parts of whole-plant corn silage, 4 parts of corn gluten meal, 14 parts of soybean meal, 4 parts of cotton seeds, 8 parts of rumen bypass fatty acid calcium, 12 parts of potassium bicarbonate, 11 parts of rumen lysine, 6 parts of methionine, 2 parts of high-yield cow premix, 18 parts of domestic alfalfa, 16 parts of wheat straw, 14 parts of millet grass, 15 parts of oat grass, 12 parts of imported alfalfa grass, 15 parts of alfalfa silage, 6 parts of water, 7 parts of pressed corn, 7 parts of brewer's grain, 3 parts of corn meal, 5 parts of cotton meal, 7 parts of beet particles, 6 parts of puffed soybean, 2 parts of urea, 2 parts of cow premix, 6 parts of dry cow premix, 7 parts of cow premix, 8 parts of cow premix, 5 parts of rumen bypass choline, 10 parts of fat powder, 1 part of yeast, 3 parts of small-size, 3 parts of soybean hull, 6 parts of erectable soybean meal, 2 parts of glucose, 1 part of peptide bond, 4 parts of mold remover, 5 parts of melon, 4 parts of muskmelon, 7 parts of lactic acid bacteria, 3 parts of milk cow premix, 2 parts of sweet water, 4 parts of sweet orange peel, and 4 parts of mixed solution.
Meanwhile, the preparation method adopts the preparation method of the high-yield dairy cow low-protein daily ration provided by the application, and finally the high-yield dairy cow low-protein daily ration of the component is obtained.
Feed example 2:
the embodiment of the application provides a low-protein daily ration for high-yield cows, which comprises the following raw materials in parts by mass: 15 parts of high-quality alfalfa, 22 parts of whole-plant corn silage, 12 parts of corn gluten meal, 14 parts of soybean meal, 4 parts of cotton seeds, 12 parts of rumen bypass fatty acid calcium, 16 parts of potassium bicarbonate, 14 parts of rumen lysine, 7 parts of methionine, 6 parts of high-yield cow premix, 20 parts of domestic alfalfa, 24 parts of wheat straw, 16 parts of millet grass, 18 parts of oat grass, 14 parts of imported alfalfa grass, 16 parts of alfalfa silage, 7 parts of water, 12 parts of corn, 8 parts of brewer's grain, 4 parts of corn meal, 8 parts of cotton meal, 9 parts of beet particles, 12 parts of puffed soybean, 3 parts of urea, 6 parts of cow premix, 13 parts of dry cow premix, 8 parts of cow premix, 10 parts of raising cow premix, 6 parts of rumen bypass choline, 13 parts of fat powder, 5 parts of yeast, 3 parts of baking soda, 10 parts of soybean hull, 11 parts of erectable soybean meal, 3 parts of glucose, 2 parts of peptide, 5 parts of mold remover, 6 parts of melon, 5 parts of muskmelon, 5 parts of lactobacillus, 4 parts of lactic acid, 7 parts of compressed amino acid, 4 parts of a mixed solution, 4 parts of a sweet solution, and 4 parts of a solution of a sweet water.
Meanwhile, the preparation method adopts the preparation method of the high-yield dairy cow low-protein daily ration provided by the application, and finally the high-yield dairy cow low-protein daily ration of the component is obtained.
Feed example 3:
the embodiment of the application provides a low-protein daily ration for high-yield cows, which comprises the following raw materials in parts by mass: 20 parts of high-quality alfalfa, 26 parts of whole-plant corn silage, 19 parts of corn gluten meal, 18 parts of soybean meal, 8 parts of whole cotton seed, 16 parts of rumen bypass fatty acid calcium, 17 parts of potassium bicarbonate, 17 parts of rumen lysine, 8 parts of methionine, 9 parts of high-yield cow premix, 22 parts of domestic alfalfa, 27 parts of wheat straw, 24 parts of millet grass, 22 parts of oat grass, 16 parts of imported alfalfa grass, 18 parts of alfalfa silage, 9 parts of water, 13 parts of pressed corn, 9 parts of brewer's grain, 8 parts of corn meal, 16 parts of cotton meal, 15 parts of beet particles, 14 parts of puffed soybean, 4 parts of urea, 9 parts of cow premix, 15 parts of dry cow premix, 12 parts of cow premix, 15 parts of cow premix, 7 parts of rumen bypass choline, 15 parts of fat powder, 6 parts of yeast, 4 parts of small-sized soda, 15 parts of soybean hulls, 12 parts of erectable soybean meal, 4 parts of glucose, 3 parts of peptide, 6 parts of mould remover, 7 parts of melon, 6 parts of muskmelon, 6 parts of lactic acid bacteria, 8 parts of milk thistle, 8 parts of sweet orange peel, 8 parts of mixed solution, 5 parts of sweet water, and 8 parts of a solution of sweet orange peel.
Meanwhile, the preparation method adopts the preparation method of the high-yield dairy cow low-protein daily ration provided by the application, and finally the high-yield dairy cow low-protein daily ration of the component is obtained.
In order to perform efficacy tests on the high-yield low-protein daily ration (feed examples 1-3) provided by the application and the existing low-protein daily ration (comparative example 1) for cows, the following biological examples were performed to obtain the specific effects of the high-yield low-protein daily ration provided by the application.
Biological example 1:
test target: normal cows, each body weight floats around 370 kg of normal cows.
The test method comprises the following steps:
the first step: preparing 20 tested target cows, dividing the target cows into two groups of 10 cows, firstly feeding the target cows with normal herbivores for 3 days, and then carrying out centralized management for subsequent feeding;
and a second step of: preparing the feeds of examples 1-3 provided by the application and the existing low-protein ration, and synchronously feeding, wherein the feeding days are continuously 30 days, and the feeding proportion of each feed per day is 50kg;
and a third step of: for normal days of 30 days, the physical state of the dairy cows is recorded, and the specific experimental results are shown in table 1.
Table 1:
analysis of results: by using the daily ration for high-yield cows, which is provided by the application, compared with the existing feed, the weight of the cow is kept obviously increased, the milk yield of the feed provided by the application is greatly improved, and secondly, by detecting the accumulation degree of liver protein, the feed provided by the application eats the accumulation degree of liver protein of the cow, which is obviously lower than that of the existing feed, so that the cow is kept in a normal healthy state, the protein content is reduced, and the kidney burden is reduced.
Biological example 2:
test target: pregnant cows, each body weight floats around 420 kg of normal cows.
The test method comprises the following steps: the results of the specific experiments are shown in Table 2 with reference to biological example 1.
Table 2:
analysis of results: by using the daily ration for high-yield cows, the milk yield of the high-yield cows can be improved compared with that of common feeds under the action of the high-yield cows, the normal weight of the pregnant cows can not be influenced, and the nutrition and health of the high-yield cows can be ensured.
Biological example 3:
test target: the weight of each obese cow floats around 500 kg of that of a normal cow.
The test method comprises the following steps: the results of the specific experiments are shown in Table 1, which refers to biological example.
Table 3:
analysis of results: by using the daily ration for high-yield cows with low protein provided by the application, when aiming at obese cows, the weight of the cows is kept, and meanwhile, the internal protein accumulation is continuously reduced, so that the protein is further reduced, and the liver health is protected.
Based on the experimental analysis of the biological examples 1-3, the application has excellent daily ration feeding effect on different cows in a cow farm by using the prepared high-yield cows with low protein.
And the effects of the high-yield dairy cow low-protein daily ration provided by the application are synchronously based, the high-yield dairy cow low-protein daily ration can be applied to accurate nutrition and carbon and nitrogen emission reduction of dairy farms, and the specific implementation steps are as follows (figure 1):
s1: selecting various feed raw materials to prepare Total Mixed Ration (TMR) with different protein levels of the high-yield dairy cows, measuring various nutritional ingredients by using a CNCPS method, measuring the digestibility of the feed by using an in vitro gas production technology, evaluating the nitrogen balance condition of rumen, and optimizing the formula structure of the ration;
s2: the method comprises the steps of comparing the change of microorganisms in rumen and manure of high-yield cows through a feeding test and high-throughput sequencing of 16S rDNA, determining marker metabolites in manure of the high-yield cows through metabonomic sequencing, determining carbon and nitrogen emission through an inversion type gas diffusion technology and an open-circuit laser device, and finally analyzing the correlation relations of the microorganisms in rumen, manure metabolites and manure with different nutrition levels and the carbon and nitrogen emission through Pearson, so as to construct an interaction network of the cows influencing the carbon and nitrogen emission of the cows through nutrition, and realize the high-quality, high-yield, high-efficiency and environment-friendly development of a farm.
Although embodiments of the present application have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the application, the scope of which is defined in the appended claims and their equivalents.

Claims (10)

1. The low-protein daily ration for the high-yield dairy cows is characterized by comprising the following raw materials in parts by weight: 10 to 20 parts of high-quality alfalfa, 7 to 26 parts of whole plant corn silage, 4 to 19 parts of corn gluten meal, 13 to 18 parts of soybean meal, 3 to 8 parts of whole cotton seeds, 4 to 16 parts of rumen bypass fatty acid calcium, 12 to 17 parts of potassium bicarbonate, 6 to 17 parts of rumen lysine, 6 to 8 parts of methionine, 2 to 9 parts of high-yield cow premix, 18 to 22 parts of domestic alfalfa, 16 to 27 parts of wheat straw, 14 to 24 parts of millet grass, 15 to 22 parts of oat grass, 12 to 16 parts of imported alfalfa, 15 to 18 parts of alfalfa silage, 6 to 9 parts of water, 7 to 13 parts of pressed corn, 7 to 9 parts of brewer's grains, 3 to 8 parts of corn meal, 5 to 16 parts of cotton meal, 7 to 15 parts of beet particles, 6 to 14 parts of puffed soybean, 2 to 4 parts of urea 2-9 parts of cow premix, 6-15 parts of dry cow premix, 7-12 parts of cow premix, 8-15 parts of cow premix, 5-7 parts of rumen bypass choline, 10-15 parts of fat powder, 1-6 parts of yeast, 3-4 parts of baking soda, 3-15 parts of soybean hulls, 6-12 parts of corn steep liquor, 1-8 parts of corn steep liquor, 2-4 parts of glucose, 1-3 parts of peptide An Jian, 3-6 parts of mildew remover, 5-7 parts of muskmelon, 4-6 parts of muskmelon, 4-15 parts of lactobacillus, 3-6 parts of amino acid, 6-11 parts of silybum marianum mixed solution, 3-8 parts of compound enzyme, 3-8 parts of soybean molasses, 3-8 parts of orange peel water, 4-5 parts of soda and 2-8 parts of deodorizing solution.
2. The low protein ration of claim 1, wherein the deodorizing solution comprises urea and humic acid in an addition ratio of 0.5:1.2, mixing and preparing.
3. The low-protein ration for high-yield cows of claim 1, wherein the silybum marianum mixed solution is prepared by mixing and distilling silybum marianum meal, myristic acid, aqueous emulsion, honeysuckle and glucose drops.
4. A method for preparing a low protein ration for high yielding cows, which is characterized by adopting the low protein ration for high yielding cows as claimed in any one of claims 1 to 3, comprising the following steps:
s1, precisely weighing high-quality prandial, whole plant corn silage, corn protein powder, soybean meal, whole cotton seed, rumen bypass fatty acid calcium, potassium bicarbonate, rumen lysine, methionine, high-yield cow premix, domestic alfalfa, wheat straw, millet grass, oat grass, imported alfalfa grass, alfalfa silage, water, pressed corn, brewer' S grains, corn flour, cotton meal, beet particles, puffed soybean, urea, cow premix, dry cow premix, rumen bypass choline, fat powder, yeast, sodium bicarbonate, soybean hulls, immediately absorbable soybean meal with tumor bypass flavor, glucose and peptide An Jian according to the formula parts, mixing in a stirring device, simultaneously adding a mold removing agent for mixing, and drying to obtain a basic mixed raw material;
s2, respectively diluting amino acid and a microbial agent with distilled water according to a ratio of 1:4, placing the obtained mixed solution into a temperature of 40-45 ℃ for intermittent stirring for 2-4 hours to obtain a bacterial solution, adding lactobacillus to carry out fluid replacement, obtaining a bacterial replacement solution, then taking melon and muskmelon to crush and squeeze juice for mixing, adding a thickening agent to obtain melon slurry, finally adding the obtained base supplementing solution into the melon slurry according to 12%, carrying out anaerobic fermentation at a constant temperature of 40-45 ℃ for 72 hours, and obtaining a liquid fermentation product containing natural low protease;
s3, filtering, concentrating, spraying and drying the obtained liquid fermentation product at low temperature, and then adding the liquid fermentation product into the basic mixed raw material for uniform mixing to obtain the low-protein ration carrier raw material;
s4, precisely weighing the silybum marianum mixed solution, synchronously placing the silybum marianum mixed solution, the soy molasses and the compound enzyme into a reaction kettle, and uniformly mixing the silybum marianum mixed solution with ultrasonic waves to obtain a mixture A;
s5, mixing orange peel water and soda water, taking a proper amount of distilled water, placing the distilled water into another reaction kettle, adding precisely weighed deodorant solution, and fully dissolving the deodorant solution by ultrasonic waves to obtain a ingredient B;
s6, pouring the ingredient A into the ingredient B, stirring while pouring, shearing at a high speed, and homogenizing in a high-pressure homogenizer to obtain an added base solution;
and S7, adding the base liquid in a drop spraying mode into the obtained low-protein daily ration carrier raw material, fully stirring and subsequently drying, and preparing the obtained raw material by a feed machine to obtain the low-protein daily ration for the high-yield dairy cows.
5. The method for preparing low protein ration for high yield cows of claim 4, wherein in step S1, the basic mixed raw material is fed into a dryer for drying treatment at 60-70 ℃ and the water content of the basic mixed raw material is stabilized at 30-35%.
6. The method according to claim 4, wherein in the step S2, the thickener is any one of carrageenan, xanthan gum, guar gum, konjac gum, locust bean gum, edible gelatin, and alginate.
7. The method of claim 4, wherein in step S4, the complex enzyme comprises any one of phytase, protease, amylase, xylanase and coenzyme.
8. The method according to claim 4, wherein in the step S6, the mixing ratio of the ingredient a to the ingredient B is 3:0.8.
9. The method for preparing low protein ration for high yield cows of claim 4, wherein in step S6, the high speed shearing rate is 18000-19000 r/min and the shearing time is 2-4 min; the homogenizing pressure of high-pressure homogenizing is 70-140 MPa, and the homogenizing times are 6-12 times.
10. The use of a high-yield low-protein ration according to claims 1-3 for accurate nutrition and carbon-nitrogen emission reduction in dairy farms.
CN202310892976.3A 2023-07-19 2023-07-19 Preparation method of low-protein daily ration for high-yield cows Pending CN116806926A (en)

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