CN116773620A - 使用生物传感器的氧化还原酶的电化学测定方法以及其中使用的生物传感器 - Google Patents

使用生物传感器的氧化还原酶的电化学测定方法以及其中使用的生物传感器 Download PDF

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Publication number
CN116773620A
CN116773620A CN202310265289.9A CN202310265289A CN116773620A CN 116773620 A CN116773620 A CN 116773620A CN 202310265289 A CN202310265289 A CN 202310265289A CN 116773620 A CN116773620 A CN 116773620A
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CN
China
Prior art keywords
mediator
oxidoreductase
substrate
reagent layer
biosensor
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Pending
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CN202310265289.9A
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English (en)
Chinese (zh)
Inventor
筱嵜耕太郎
大贺美咲
三上寿幸
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Arkray Inc
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Arkray Inc
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Publication of CN116773620A publication Critical patent/CN116773620A/zh
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value ; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue
    • A61B5/14532—Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value ; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue for measuring glucose, e.g. by tissue impedance measurement
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
    • G01N27/3272—Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value ; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue
    • A61B5/1468—Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value ; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue using chemical or electrochemical methods, e.g. by polarographic means
    • A61B5/1486—Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value ; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue using chemical or electrochemical methods, e.g. by polarographic means using enzyme electrodes, e.g. with immobilised oxidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/004—Enzyme electrodes mediator-assisted
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/48—Systems using polarography, i.e. measuring changes in current under a slowly-varying voltage

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Physics & Mathematics (AREA)
  • Electrochemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Emergency Medicine (AREA)
  • Surgery (AREA)
  • Veterinary Medicine (AREA)
  • Medical Informatics (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Biomedical Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Optics & Photonics (AREA)
  • Public Health (AREA)
  • Hematology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202310265289.9A 2022-03-17 2023-03-16 使用生物传感器的氧化还原酶的电化学测定方法以及其中使用的生物传感器 Pending CN116773620A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2022042572A JP7781686B2 (ja) 2022-03-17 2022-03-17 バイオセンサを用いた酸化還元酵素の電気化学的測定方法及びそれに用いるバイオセンサ
JP2022-042572 2022-03-17

Publications (1)

Publication Number Publication Date
CN116773620A true CN116773620A (zh) 2023-09-19

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CN202310265289.9A Pending CN116773620A (zh) 2022-03-17 2023-03-16 使用生物传感器的氧化还原酶的电化学测定方法以及其中使用的生物传感器

Country Status (4)

Country Link
US (1) US20230293059A1 (https=)
EP (1) EP4245857A1 (https=)
JP (1) JP7781686B2 (https=)
CN (1) CN116773620A (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117030820A (zh) * 2023-09-28 2023-11-10 艾康生物技术(杭州)有限公司 一种生物传感器的测量方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2026022017A (ja) * 2024-07-30 2026-02-12 キッコーマン株式会社 試薬組成物及びセンサ並びにその製造方法

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JP2901678B2 (ja) 1988-03-15 1999-06-07 ライフ―チェック ラボラトリーズ 電流測定による診断分析の方法及び装置
US5243516A (en) 1989-12-15 1993-09-07 Boehringer Mannheim Corporation Biosensing instrument and method
DE4003194A1 (de) * 1990-02-03 1991-08-08 Boehringer Mannheim Gmbh Verfahren und sensorelektrodensystem zur elektrochemischen bestimmung eines analyts oder einer oxidoreduktase sowie verwendung hierfuer geeigneter verbindungen
JPH07101215B2 (ja) 1990-04-11 1995-11-01 国立身体障害者リハビリテーションセンター総長 生体機能物質固定化電極を用いた分析法
US5366609A (en) 1993-06-08 1994-11-22 Boehringer Mannheim Corporation Biosensing meter with pluggable memory key
AUPN363995A0 (en) 1995-06-19 1995-07-13 Memtec Limited Electrochemical cell
JP4088312B2 (ja) 2003-10-30 2008-05-21 アークレイ株式会社 バイオセンサおよびその製造方法
JP2007225305A (ja) * 2006-02-21 2007-09-06 Denso Corp 酵素電極
BRPI0717620A2 (pt) 2006-10-24 2013-10-22 Bayer Healthcare Llc Amperometria de decaimento transitório
JP5584740B2 (ja) 2011-09-26 2014-09-03 アークレイ株式会社 グルコースセンサ
KR101239381B1 (ko) * 2012-05-02 2013-03-05 주식회사 아이센스 산화환원반응용 시약의 안정제 조성물
CN109690304A (zh) 2016-07-12 2019-04-26 安晟信医疗科技控股公司 通过使用来自两个电极的交替输出信号进行电化学分析的方法
JP6713364B2 (ja) 2016-07-20 2020-06-24 アークレイ株式会社 アスコルビン酸応答電極およびバイオセンサ
KR102372113B1 (ko) * 2016-10-05 2022-03-07 에프. 호프만-라 로슈 아게 다중 분석물 진단 테스트 엘리먼트들을 위한 검출 시약들 및 전극 배열들, 그리고 그것을 사용하는 방법들

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117030820A (zh) * 2023-09-28 2023-11-10 艾康生物技术(杭州)有限公司 一种生物传感器的测量方法
CN117030820B (zh) * 2023-09-28 2024-01-09 艾康生物技术(杭州)有限公司 一种生物传感器的测量方法

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Publication number Publication date
JP2023136725A (ja) 2023-09-29
EP4245857A1 (en) 2023-09-20
JP7781686B2 (ja) 2025-12-08
US20230293059A1 (en) 2023-09-21

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