CN116745433A - 采用多联体的分析物检测方法 - Google Patents
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- CN116745433A CN116745433A CN202180087675.2A CN202180087675A CN116745433A CN 116745433 A CN116745433 A CN 116745433A CN 202180087675 A CN202180087675 A CN 202180087675A CN 116745433 A CN116745433 A CN 116745433A
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/151—Modifications characterised by repeat or repeated sequences, e.g. VNTR, microsatellite, concatemer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/191—Modifications characterised by incorporating an adaptor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
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- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
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- C—CHEMISTRY; METALLURGY
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
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| GBGB2018503.9A GB202018503D0 (en) | 2020-11-25 | 2020-11-25 | Analyte detection method employing concatamers |
| GB2018503.9 | 2020-11-25 | ||
| PCT/EP2021/082775 WO2022112300A1 (en) | 2020-11-25 | 2021-11-24 | Analyte detection method employing concatemers |
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| CN116745433A true CN116745433A (zh) | 2023-09-12 |
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| IL (1) | IL303093A (https=) |
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| WO2025012226A1 (en) | 2023-07-12 | 2025-01-16 | Olink Proteomics Ab | Method, components and software for detecting a systematic error in a protein detection system |
| WO2025031642A1 (en) | 2023-08-07 | 2025-02-13 | Olink Proteomics Ab | A method for determining run errors for an analysis-method for quantifying a plurality of analytes in a plurality of biological samples |
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| WO2025262178A1 (en) | 2024-06-18 | 2025-12-26 | Olink Proteomics Ab | System and method for detecting analytes in a sample |
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| AU2005225057A1 (en) * | 1999-03-26 | 2005-12-01 | Bp Corporation North America Inc. | Synthetic ligation reassembly in directed evolution |
| SE516272C2 (sv) | 2000-02-18 | 2001-12-10 | Ulf Landegren | Metoder och kit för analytdetektion mha proximitets-probning |
| JP2005509444A (ja) | 2001-11-23 | 2005-04-14 | シモン・フレデリックソン | 多価近接プローブにより近接プロービングするための方法およびキット |
| CN101410530B (zh) | 2003-04-18 | 2013-03-27 | 贝克顿·迪金森公司 | 免疫-扩增 |
| WO2005079357A2 (en) * | 2004-02-17 | 2005-09-01 | Dana Farber Cancer Institute | Nucleic acid representations utilizing type iib restriction endonuclease cleavage products |
| US7914987B2 (en) | 2004-06-14 | 2011-03-29 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and compositions for use in analyte detection using proximity probes |
| DK1842226T3 (da) | 2004-11-03 | 2010-10-18 | Iris Molecular Diagnostics Inc | Homogen analytpåvisning |
| GB0605584D0 (en) | 2006-03-20 | 2006-04-26 | Olink Ab | Method for analyte detection using proximity probes |
| GB201101621D0 (en) | 2011-01-31 | 2011-03-16 | Olink Ab | Method and product |
| GB201201547D0 (en) | 2012-01-30 | 2012-03-14 | Olink Ab | Method and product |
| US11898141B2 (en) * | 2014-05-27 | 2024-02-13 | The Broad Institute, Inc. | High-throughput assembly of genetic elements |
| GB201518655D0 (en) | 2015-10-21 | 2015-12-02 | Olink Ab | Method for generating proximity probes |
| CN109844133A (zh) * | 2016-08-15 | 2019-06-04 | 安可济控股有限公司 | 检测罕见序列变体的组合物和方法 |
| WO2018108328A1 (en) * | 2016-12-16 | 2018-06-21 | F. Hoffmann-La Roche Ag | Method for increasing throughput of single molecule sequencing by concatenating short dna fragments |
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| JP2023550568A (ja) | 2023-12-01 |
| WO2022112300A1 (en) | 2022-06-02 |
| CA3199169A1 (en) | 2022-06-02 |
| US20220162589A1 (en) | 2022-05-26 |
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| AU2021388789A9 (en) | 2025-03-27 |
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| EP4251762A1 (en) | 2023-10-04 |
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| GB202018503D0 (en) | 2021-01-06 |
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