CN116711805A - Preparation method of rice protein - Google Patents
Preparation method of rice protein Download PDFInfo
- Publication number
- CN116711805A CN116711805A CN202310663380.6A CN202310663380A CN116711805A CN 116711805 A CN116711805 A CN 116711805A CN 202310663380 A CN202310663380 A CN 202310663380A CN 116711805 A CN116711805 A CN 116711805A
- Authority
- CN
- China
- Prior art keywords
- rice
- rice protein
- raw materials
- protein
- putting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 112
- 235000009566 rice Nutrition 0.000 title claims abstract description 112
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 60
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 60
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 111
- 239000002994 raw material Substances 0.000 claims abstract description 79
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 48
- 238000000034 method Methods 0.000 claims abstract description 28
- 239000007788 liquid Substances 0.000 claims abstract description 16
- 238000002386 leaching Methods 0.000 claims abstract description 14
- 239000012670 alkaline solution Substances 0.000 claims abstract description 8
- 239000000243 solution Substances 0.000 claims abstract description 8
- 238000001035 drying Methods 0.000 claims description 35
- 239000006228 supernatant Substances 0.000 claims description 32
- 239000000843 powder Substances 0.000 claims description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 27
- 238000000227 grinding Methods 0.000 claims description 21
- 238000000926 separation method Methods 0.000 claims description 18
- 239000003513 alkali Substances 0.000 claims description 17
- 238000010438 heat treatment Methods 0.000 claims description 14
- 238000010298 pulverizing process Methods 0.000 claims description 13
- 230000027939 micturition Effects 0.000 claims description 4
- 239000011259 mixed solution Substances 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims 2
- 239000012535 impurity Substances 0.000 abstract description 16
- 238000000605 extraction Methods 0.000 abstract description 9
- 239000002351 wastewater Substances 0.000 abstract description 6
- 239000010865 sewage Substances 0.000 abstract description 3
- 235000013339 cereals Nutrition 0.000 description 23
- 239000000203 mixture Substances 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 6
- 108090000765 processed proteins & peptides Proteins 0.000 description 4
- 230000001105 regulatory effect Effects 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- 239000004382 Amylase Substances 0.000 description 2
- 102000013142 Amylases Human genes 0.000 description 2
- 108010065511 Amylases Proteins 0.000 description 2
- 108090000145 Bacillolysin Proteins 0.000 description 2
- 108010059892 Cellulase Proteins 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 102000004882 Lipase Human genes 0.000 description 2
- 108090001060 Lipase Proteins 0.000 description 2
- 239000004367 Lipase Substances 0.000 description 2
- 102000035092 Neutral proteases Human genes 0.000 description 2
- 108091005507 Neutral proteases Proteins 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- 102000004139 alpha-Amylases Human genes 0.000 description 2
- 108090000637 alpha-Amylases Proteins 0.000 description 2
- 229940024171 alpha-amylase Drugs 0.000 description 2
- 235000019418 amylase Nutrition 0.000 description 2
- 229940106157 cellulase Drugs 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 235000019421 lipase Nutrition 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 235000008452 baby food Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000015243 ice cream Nutrition 0.000 description 1
- 238000003754 machining Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 102000035118 modified proteins Human genes 0.000 description 1
- 108091005573 modified proteins Proteins 0.000 description 1
- LPUQAYUQRXPFSQ-DFWYDOINSA-M monosodium L-glutamate Chemical compound [Na+].[O-]C(=O)[C@@H](N)CCC(O)=O LPUQAYUQRXPFSQ-DFWYDOINSA-M 0.000 description 1
- 235000013923 monosodium glutamate Nutrition 0.000 description 1
- 239000004223 monosodium glutamate Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000000751 protein extraction Methods 0.000 description 1
- 230000007065 protein hydrolysis Effects 0.000 description 1
- 239000012460 protein solution Substances 0.000 description 1
- 235000011962 puddings Nutrition 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/12—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from cereals, wheat, bran, or molasses
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Peptides Or Proteins (AREA)
Abstract
The application discloses a preparation method of rice protein, which comprises the steps of taking broken rice, old rice, lautness rice, polished round-grained rice or glutinous rice as raw materials, extracting by an alkaline method to prepare the rice protein, wherein the alkaline solution is NaOH solution, the mass fraction of the alkaline solution is 0.25-0.30%, the leaching time is 5-6 h, the leaching temperature is 30-40 ℃, and the material-liquid ratio is 1: 8-1: 10. the application optimizes the process in the process of extracting the rice protein by an alkaline method, takes the extraction rate of the rice protein as an index, ensures that the extraction rate of the rice protein is more than 85 percent, reduces the content of organic matters in impurities generated in the preparation process, reduces the COD value in waste water and reduces the burden of subsequent sewage treatment while ensuring the extraction rate of the rice protein.
Description
Technical Field
The application belongs to the technical field of rice finish machining, and particularly relates to a preparation method of rice protein.
Background
At present, rice proteins are more studied, and the rice proteins are more and more widely applied, such as rice protein powder production, rice protein hydrolysis, rice modified protein, high added value peptide, bioactive peptide and resistant protein production; as additives for various foods, such as mixed drinks, puddings, ice cream, infant foods, etc. The rice protein not only has unique nutrition function, but also has the health care function of reducing the cholesterol content in serum and the like.
Chinese patent CN1417344C, published in 2003, discloses a new process for extracting rice protein from rice grains, characterized in that the ratio of byproduct rice grains and complex enzyme in monosodium glutamate factory is 1000:1.5-4.5 by weight; wherein the complex enzyme is composed of amylase 0.05% -0.15%, lipase 0.025% -0.075%, cellulase 0.025% -0.075% and protease 0.05% -0.15%; the process is as follows: adding rice grains into a container, regulating the pH to 3 by hydrochloric acid, regulating the temperature to 35-40 ℃, adding cellulase according to the proportion, reacting for 30 minutes, adding 30% NaOH, regulating the pH to 5.0-5.5, adding lipase, reacting for 30 minutes, adding NaOH to regulate the pH to neutral 7.0, adding amylase and protease, reacting for 20 minutes, heating to 60-70 ℃, reacting for 30 minutes, filtering, adding dilute alkali solution with the pH of 9.5 into filter residues for dissolving, controlling the temperature below 60 ℃ to obtain protein solution, regulating the pH to 5.5 by acid, precipitating protein, and obtaining the concentrated rice protein with the content of 75%.
Another chinese patent CN1257984C, published in 2006, discloses a method for preparing rice protein peptide, which is characterized in that rice residue and water are mixed according to a weight ratio of 1:4-6, the temperature is 55-60 ℃, the temperature is kept for 30 minutes, then, according to the activity of enzyme preparation being 50,000U/mg, alpha-amylase and neutral protease which are 0.05% -0.1% of the weight of the rice residue are respectively added, wherein the weight ratio of the alpha-amylase to the neutral protease is 1:1, stirring is carried out for 5-7 hours at ph6.5-7.5 and the temperature is 50-60 ℃, the reactant is filtered, and the obtained liquid is spray-dried, so that white powder product is obtained, wherein the content of the rice protein peptide is about 80%.
At present, a better preparation method of rice protein is still required to be continuously developed so as to improve the protein extraction rate; in the preparation process of the rice egg port, the wastewater is mainly separated impurities. The impurities contain certain organic matters such as starch, protein and the like, and the organic matters, especially the protein, directly influence the COD value of the wastewater, thereby increasing the load of subsequent sewage treatment.
Disclosure of Invention
Aiming at the problems in the prior art, the application provides the following technical scheme:
a preparation method of rice protein is characterized in that broken rice, old rice, lautness rice, polished round-grained rice or glutinous rice are used as raw materials to prepare the rice protein by an alkaline method, alkaline solution is NaOH solution, the mass fraction of the alkaline solution is 0.25% -0.30%, the leaching time is 5-6 h, the leaching temperature is 30-40 ℃, and the feed-liquid ratio is 1: 8-1: 10.
a preparation method of rice protein comprises the following steps:
s1, primary crushing
The raw materials are put into a pulverizer, and the raw materials are primarily pulverized and then collected by the pulverizer;
s2, superfine grinding
Putting the raw materials after the preliminary grinding of S1 into a grinder for secondary grinding, and then collecting ground raw material powder;
s3, alkali liquor leaching
Putting the raw material powder ground in the step S2 into a jacketed kettle, adding alkali liquor and water, and heating for a certain time by using steam to obtain a mixed solution;
s4, centrifugal separation
Putting the mixed liquor obtained after leaching in the step S3 into a centrifuge for centrifugal separation to obtain primarily separated rice dreg liquid;
s5, extracting supernatant
Separating the rice dreg liquid in the step S4 by a plate-frame filter to obtain a supernatant;
s6, air flow drying
And (3) carrying out air flow drying on the supernatant obtained in the step (S5), and obtaining the rice protein after drying.
In the above-described preferred embodiment, the preliminary pulverization requires passing through a 90-mesh screen.
Preferably, in the above technical scheme, the superfine grinding requires passing through a 180-mesh screen.
As a preferable mode of the technical scheme, the condition of centrifugal separation is 3000r/min, and the centrifugal time is 20min.
As the optimization of the technical scheme, the air flow drying adopts a blast constant temperature drying oven, the air outlet temperature is 85 ℃, so that the water content is below 5%, and the rice protein is obtained.
As a preferable mode of the above technical scheme, the determination of the protein content adopts a double urination method to determine the protein content in the supernatant:
the beneficial effects of the application are as follows:
1: the application optimizes the process in the process of extracting the rice protein by an alkaline method, and takes the extraction rate of the rice protein as an index, so that the extraction rate of the rice protein is more than 85 percent.
2: the extraction rate of the rice protein is ensured, the content of organic matters in impurities generated in the preparation process is reduced, the COD value in the wastewater is reduced, and the burden of subsequent sewage treatment is reduced.
Drawings
FIG. 1 is a process flow diagram of a method for preparing rice protein according to the present application.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present application more apparent, the technical solutions of the present application will be clearly and completely described below with reference to the embodiments.
A preparation method of rice protein is characterized in that broken rice, old rice, lautness rice, polished round-grained rice or glutinous rice are used as raw materials to prepare the rice protein by an alkaline method, alkaline solution is NaOH solution, the mass fraction of the alkaline solution is 0.25% -0.30%, the leaching time is 5-6 h, the leaching temperature is 30-40 ℃, and the feed-liquid ratio is 1: 8-1: 10.
the method comprises the following steps:
s1, primary crushing
The raw materials are put into a pulverizer, and the raw materials are primarily pulverized and then collected by the pulverizer;
s2, superfine grinding
Putting the raw materials after the preliminary grinding of S1 into a grinder for secondary grinding, and then collecting ground raw material powder;
s3, alkali liquor leaching
Putting the raw material powder ground in the step S2 into a jacketed kettle, adding alkali liquor and water, and heating for a certain time by using steam to obtain a mixed solution;
s4, centrifugal separation
Putting the mixed liquor obtained after leaching in the step S3 into a centrifuge for centrifugal separation to obtain primarily separated rice dreg liquid;
s5, extracting supernatant
Separating the rice dreg liquid in the step S4 by a plate-frame filter to obtain a supernatant;
s6, air flow drying
And (3) carrying out air flow drying on the supernatant obtained in the step (S5), and obtaining the rice protein after drying.
In the primary pulverization, a 90 mesh screen is required.
The superfine grinding requires passing through a 180 mesh screen.
The centrifugal separation condition is 3000r/min, and the centrifugal time is 20min.
The air flow drying adopts a blast constant temperature drying oven, the air outlet temperature is 85 ℃, so that the water content is below 5%, and the rice protein is obtained.
The protein content is determined by adopting a double urination method, and the protein content in supernatant is determined:
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
Example 1
Putting raw materials into a pulverizer, operating the pulverizer for 10min, primarily pulverizing the raw materials, collecting the raw materials through a 90-mesh screen, putting the primarily pulverized raw materials into a grinder for secondary grinding, then collecting ground raw material powder through a 180-mesh screen, putting the ground raw material powder into a jacketed kettle, adding NaOH alkali liquor with the mass fraction of 0.25% and water with the mass fraction of 1:8, heating the mixture to 30 ℃ with steam for 5h to obtain mixed liquor, putting the mixed liquor into a centrifuge for centrifugal separation under the centrifugal condition of 3000r/min for 20min to obtain primarily separated rice grain liquor, separating the rice grain liquor through a plate and frame filter to obtain supernatant and impurities, and carrying out air flow drying on the supernatant through an air flow constant temperature drying box, wherein the air outlet temperature is 85 ℃ to ensure that the water content is below 5%, thus obtaining rice protein.
Example 2
Putting raw materials into a pulverizer, operating the pulverizer for 10min, primarily pulverizing the raw materials, collecting the raw materials through a 90-mesh screen, putting the primarily pulverized raw materials into a grinder for secondary grinding, then collecting ground raw material powder through a 180-mesh screen, putting the ground raw material powder into a jacketed kettle, adding NaOH alkali liquor with the mass fraction of 0.28% and water with the mass fraction of 1:8, heating the mixture to 30 ℃ with steam for 5h to obtain mixed liquor, putting the mixed liquor into a centrifuge for centrifugal separation under the centrifugal condition of 3000r/min for 20min to obtain primarily separated rice grain liquor, separating the rice grain liquor through a plate-frame filter to obtain supernatant and impurities, and carrying out air flow drying on the supernatant through an air flow constant temperature drying box, wherein the air outlet temperature is 85 ℃ to ensure that the water content is below 5%, thus obtaining rice protein.
Example 3
Putting raw materials into a pulverizer, operating the pulverizer for 10min, primarily pulverizing the raw materials, collecting the raw materials through a 90-mesh screen, putting the primarily pulverized raw materials into a grinder for secondary grinding, then collecting ground raw material powder through a 180-mesh screen, putting the ground raw material powder into a jacketed kettle, adding NaOH alkali liquor with the mass fraction of 0.30% and water with the mass fraction of 1:8, heating the mixture to 30 ℃ with steam for 5h to obtain mixed liquor, putting the mixed liquor into a centrifuge for centrifugal separation under the centrifugal condition of 3000r/min for 20min to obtain primarily separated rice grain liquor, separating the rice grain liquor through a plate and frame filter to obtain supernatant and impurities, and carrying out air flow drying on the supernatant through an air flow constant temperature drying box, wherein the air outlet temperature is 85 ℃ to ensure that the water content is below 5%, thus obtaining rice protein.
Example 4
Putting raw materials into a pulverizer, operating the pulverizer for 10min, primarily pulverizing the raw materials, collecting the raw materials through a 90-mesh screen, putting the primarily pulverized raw materials into a grinder for secondary grinding, then collecting ground raw material powder through a 180-mesh screen, putting the ground raw material powder into a jacketed kettle, adding NaOH alkali liquor with the mass fraction of 0.28% and water with the mass fraction of 1:9, heating the mixture to 30 ℃ with steam for 5h to obtain mixed liquor, putting the mixed liquor into a centrifuge for centrifugal separation under the centrifugal condition of 3000r/min for 20min to obtain primarily separated rice grain liquor, separating the rice grain liquor through a plate-frame filter to obtain supernatant and impurities, and carrying out air flow drying on the supernatant through an air flow constant temperature drying box, wherein the air outlet temperature is 85 ℃ to ensure that the water content is below 5%, thus obtaining rice protein.
Example 5
Putting raw materials into a pulverizer, operating the pulverizer for 10min, primarily pulverizing the raw materials, collecting the raw materials through a 90-mesh screen, putting the primarily pulverized raw materials into a grinder for secondary grinding, then collecting ground raw material powder through a 180-mesh screen, putting the ground raw material powder into a jacketed kettle, adding NaOH alkali liquor with the mass fraction of 0.25% and water with the feed liquid ratio of 1:10, heating the mixture to 30 ℃ for 5h by using steam to obtain mixed liquor, putting the mixed liquor into a centrifuge for centrifugal separation under the centrifugal condition of 3000r/min for 20min to obtain primarily separated rice grain liquor, separating the rice grain liquor through a plate-frame filter to obtain supernatant and impurities, and carrying out air flow drying on the supernatant through a blast constant temperature drying box at the air outlet temperature of 85 ℃ to ensure that the water content is below 5% to obtain rice protein.
Example 6
Putting raw materials into a pulverizer, operating the pulverizer for 10min, primarily pulverizing the raw materials, collecting the raw materials through a 90-mesh screen, putting the primarily pulverized raw materials into a grinder for secondary grinding, then collecting ground raw material powder through a 180-mesh screen, putting the ground raw material powder into a jacketed kettle, adding NaOH alkali liquor with the mass fraction of 0.25% and water with the mass fraction of 1:8, heating the mixture to 35 ℃ with steam, keeping the temperature for 5h to obtain mixed liquor, putting the mixed liquor into a centrifuge for centrifugal separation under the centrifugal condition of 3000r/min for 20min to obtain primarily separated rice grain liquid, separating the rice grain liquid through a plate and frame filter to obtain supernatant and impurities, and carrying out air flow drying on the supernatant through an air flow constant temperature drying box, wherein the air outlet temperature is 85 ℃ to ensure that the water content is below 5%, thus obtaining rice protein.
Example 7
Putting raw materials into a pulverizer, operating the pulverizer for 10min, primarily pulverizing the raw materials, collecting the raw materials through a 90-mesh screen, putting the primarily pulverized raw materials into a grinder for secondary grinding, then collecting ground raw material powder through a 180-mesh screen, putting the ground raw material powder into a jacketed kettle, adding NaOH alkali liquor with the mass fraction of 0.25% and water with the mass fraction of 1:8, heating the mixture to 40 ℃ for 5h by using steam to obtain mixed liquor, putting the mixed liquor into a centrifuge for centrifugal separation under the centrifugal condition of 3000r/min for 20min to obtain primarily separated rice grain liquor, separating the rice grain liquor through a plate-frame filter to obtain supernatant and impurities, and carrying out air flow drying on the supernatant through a blast constant temperature drying box at the air outlet temperature of 85 ℃ to ensure that the water content is below 5% to obtain rice protein.
Example 8
Putting raw materials into a pulverizer, operating the pulverizer for 10min, primarily pulverizing the raw materials, collecting the raw materials through a 90-mesh screen, putting the primarily pulverized raw materials into a grinder for secondary grinding, then collecting ground raw material powder through a 180-mesh screen, putting the ground raw material powder into a jacketed kettle, adding NaOH alkali liquor with the mass fraction of 0.25% and water with the feed liquid ratio of 1:8, heating the mixture to 30 ℃ with steam for 5.5h to obtain mixed liquor, putting the mixed liquor into a centrifuge for centrifugal separation under the centrifugal condition of 3000r/min for 20min to obtain primarily separated rice grain liquor, separating the rice grain liquor through a plate frame to obtain supernatant and impurities, and carrying out air flow drying on the supernatant through an air flow constant temperature drying oven at the air outlet temperature of 85 ℃ to ensure that the water content is below 5%, thereby obtaining rice protein.
Example 9
Putting raw materials into a pulverizer, operating the pulverizer for 10min, primarily pulverizing the raw materials, collecting the raw materials through a 90-mesh screen, putting the primarily pulverized raw materials into a grinder for secondary grinding, then collecting ground raw material powder through a 180-mesh screen, putting the ground raw material powder into a jacketed kettle, adding NaOH alkali liquor with the mass fraction of 0.25% and water with the mass fraction of 1:8, heating the mixture to 30 ℃ with steam for 6h to obtain mixed liquor, putting the mixed liquor into a centrifuge for centrifugal separation under the centrifugal condition of 3000r/min for 20min to obtain primarily separated rice grain liquor, separating the rice grain liquor through a plate and frame filter to obtain supernatant and impurities, and carrying out air flow drying on the supernatant through an air flow constant temperature drying box, wherein the air outlet temperature is 85 ℃ to ensure that the water content is below 5%, thus obtaining rice protein.
Example 10
Putting raw materials into a pulverizer, operating the pulverizer for 10min, primarily pulverizing the raw materials, collecting the raw materials through a 90-mesh screen, putting the primarily pulverized raw materials into a grinder for secondary grinding, then collecting ground raw material powder through a 180-mesh screen, putting the ground raw material powder into a jacketed kettle, adding NaOH alkali liquor with the mass fraction of 0.28% and water with the feed liquid ratio of 1:9, heating the mixture to 35 ℃ by steam for 5.5h to obtain mixed liquor, putting the mixed liquor into a centrifuge for centrifugal separation under the centrifugal condition of 3000r/min for 20min to obtain primarily separated rice grain liquor, separating the rice grain liquor through a plate frame to obtain supernatant and impurities, and carrying out air flow drying on the supernatant through an air flow constant temperature drying oven at the air outlet temperature of 85 ℃ to ensure that the water content is below 5% to obtain rice protein.
Comparative example 1
The rice protein prepared by adopting the method of Chinese patent CN 1417344C.
Comparative example 2
The rice protein prepared by adopting the method of Chinese patent CN 1257984C.
The rice proteins prepared in examples 1 to 10 and comparative examples 1 to 2 were subjected to detection of extraction rate according to the double urination method; the COD content of the impurity wastewater in the preparation process of examples 1-10 and comparative examples 1-2 was measured according to GB11914-89, and the results are shown in Table 1:
TABLE 1
As is clear from the above, the extraction rate of the rice protein prepared in the examples 1-10 is greater than that of the comparative examples 1 and 2, and the extraction rate of the rice protein is maintained to be greater than 85%, and the COD content of the impurity wastewater in the examples 1-10 can reach the standard of less than or equal to 500mg/L in GB11914-89, while the standard cannot be reached in the comparative examples 1 and 2.
The above embodiments are only for illustrating the technical solution of the present application, and are not limiting.
Claims (7)
1. A preparation method of rice protein is characterized in that broken rice, old rice, lautness rice, polished round-grained rice or glutinous rice are used as raw materials to prepare the rice protein by an alkaline method, alkaline solution is NaOH solution, the mass fraction of the alkaline solution is 0.25% -0.30%, the leaching time is 5-6 h, the leaching temperature is 30-40 ℃, and the feed-liquid ratio is 1: 8-1: 10.
2. the method for preparing rice protein according to claim 1, comprising the steps of:
s1, primary crushing
The raw materials are put into a pulverizer, and the raw materials are primarily pulverized and then collected by the pulverizer;
s2, superfine grinding
Putting the raw materials after the preliminary grinding of S1 into a grinder for secondary grinding, and then collecting ground raw material powder;
s3, alkali liquor leaching
Putting the raw material powder ground in the step S2 into a jacketed kettle, adding alkali liquor and water, and heating for a certain time by using steam to obtain a mixed solution;
s4, centrifugal separation
Putting the mixed liquor obtained after leaching in the step S3 into a centrifuge for centrifugal separation to obtain primarily separated rice dreg liquid;
s5, extracting supernatant
Separating the rice dreg liquid in the step S4 by a plate-frame filter to obtain a supernatant;
s6, air flow drying
And (3) carrying out air flow drying on the supernatant obtained in the step (S5), and obtaining the rice protein after drying.
3. A method of producing rice protein as defined in claim 2, wherein said preliminary pulverization requires passing through a 90 mesh screen.
4. A method of preparing rice protein according to claim 2, wherein the micronization requires passing through a 180 mesh screen.
5. The method for preparing rice protein according to claim 2, wherein the centrifugation condition is 3000r/min and the centrifugation time is 20min.
6. The method for preparing rice protein according to claim 2, wherein the air-flow drying is carried out by adopting an air-blast constant-temperature drying oven, and the air-out temperature is 85 ℃ so that the water content is below 5%, thereby obtaining the rice protein.
7. The method for preparing rice protein according to claim 1, wherein the protein content is determined by a double urination method, and the protein content in the supernatant is determined by:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310663380.6A CN116711805A (en) | 2023-06-06 | 2023-06-06 | Preparation method of rice protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310663380.6A CN116711805A (en) | 2023-06-06 | 2023-06-06 | Preparation method of rice protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116711805A true CN116711805A (en) | 2023-09-08 |
Family
ID=87865346
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310663380.6A Pending CN116711805A (en) | 2023-06-06 | 2023-06-06 | Preparation method of rice protein |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116711805A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1900123A (en) * | 2006-07-05 | 2007-01-24 | 江南大学 | Process for preparing rice starch and rice protein |
| CN102370044A (en) * | 2011-09-30 | 2012-03-14 | 哈尔滨工业大学 | Extraction method of high-digestibility rice protein |
| CN104814245A (en) * | 2015-04-03 | 2015-08-05 | 安徽天利粮油集团股份有限公司 | A complex extraction method for rice protein |
| CN105777861A (en) * | 2016-05-26 | 2016-07-20 | 佛山科学技术学院 | Cowage protein extraction method |
| CN111217888A (en) * | 2018-11-26 | 2020-06-02 | 罗爱芹 | Technology for extracting rice protein by alkaline method |
-
2023
- 2023-06-06 CN CN202310663380.6A patent/CN116711805A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1900123A (en) * | 2006-07-05 | 2007-01-24 | 江南大学 | Process for preparing rice starch and rice protein |
| CN102370044A (en) * | 2011-09-30 | 2012-03-14 | 哈尔滨工业大学 | Extraction method of high-digestibility rice protein |
| CN104814245A (en) * | 2015-04-03 | 2015-08-05 | 安徽天利粮油集团股份有限公司 | A complex extraction method for rice protein |
| CN105777861A (en) * | 2016-05-26 | 2016-07-20 | 佛山科学技术学院 | Cowage protein extraction method |
| CN111217888A (en) * | 2018-11-26 | 2020-06-02 | 罗爱芹 | Technology for extracting rice protein by alkaline method |
Non-Patent Citations (1)
| Title |
|---|
| 翟晓娜，等: "菜籽饼粕蛋白的提取、功能特性及其在食品中应用的研究进展", 《食品工业科技》, vol. 42, no. 12, 31 December 2021 (2021-12-31), pages 389 - 397 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10154679B2 (en) | Protein concentrate and an aqueous stream containing water-soluble carbohydrates | |
| US4804545A (en) | Production of beta-glucan, bran, protein, oil and maltose syrup from waxy barley | |
| KR890000913B1 (en) | Process for producing alcohol by fermentation without cooking | |
| CN104223125B (en) | A kind of method preparing potato residues dietary fiber | |
| CN102229643A (en) | Method for preparing high-purity rice protein and high-purity rice peptide | |
| CN102030773B (en) | Technique for coproducing phytic acid and oligopeptide from defatted rice bran | |
| MX2008000673A (en) | Corn wet milling process. | |
| CN102115690A (en) | Method for comprehensively utilizing rice bran | |
| EP0369818A2 (en) | Process for producing proteinrich product, fibrous product and/or vegetable oil from brewer's spent grain | |
| KR102493618B1 (en) | Manufacturing method of rice protein and rice starch | |
| CN115369104B (en) | Method for refining pepsin | |
| CN103146782B (en) | Method for preparing glucose syrup by using rice bran as raw material | |
| WO2023134467A1 (en) | Method for preparing starch and non-thermal-denaturation protein powder by using rice as raw material | |
| CN111955591A (en) | Method for preparing antioxidant rice protein by using rice residues | |
| CN110684074A (en) | Method for synchronously preparing maltose syrup, rice protein and rice residue protein by using rice wine residues and rice protein | |
| CN101434979A (en) | Method for extracting protein from cereal fine powder | |
| CN102020856A (en) | Method for recovering glucomannan and protein in konjac flying powder by enzyme process | |
| CN116711805A (en) | Preparation method of rice protein | |
| EP0192677B1 (en) | Processes for recovery of products from waxy barley | |
| CN116694717A (en) | Process for extracting protein from rice grains and product thereof | |
| CN117186264A (en) | Green and efficient highland barley beta-glucan extraction method | |
| CN1834106B (en) | Fermenting free hydralysis process for extracting saporin from yellow ginger | |
| CN110897059A (en) | Method for preparing plant hydrolyzed protein beverage by using wheat germs | |
| CN101319205B (en) | Process for preparing food-grade high-vitality beta-diastase | |
| CN113234786B (en) | Method for extracting selenium-enriched polypeptide from selenium-enriched rice processing byproducts |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |