CN105777861A - Cowage protein extraction method - Google Patents
Cowage protein extraction method Download PDFInfo
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- CN105777861A CN105777861A CN201610357089.6A CN201610357089A CN105777861A CN 105777861 A CN105777861 A CN 105777861A CN 201610357089 A CN201610357089 A CN 201610357089A CN 105777861 A CN105777861 A CN 105777861A
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- semen stizolobii
- stizolobii capitati
- protein
- albumen
- extracting method
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
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- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
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- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention provides a cowage protein extraction method.The method comprises the following steps that cowage is smashed and then screened through a 100-mesh screen mesh, the screened raw materials are added into a NaOH solution on the basis that the mass ratio is 1:15-25, stirring and extraction are performed on the condition of 40-50 DEG C, an extract solution is centrifuged, and a supernate is collected to obtain a cowage protein solution.The cowage protein extraction method is easy to operate, high in cowage protein yield, low in protein denaturation rate, short in protein extraction period, high in yield, low in extraction cost and suitable for large-scale extraction of cowage protein.
Description
Technical field
The present invention relates to extraction process technology field, particularly relate to the extracting method of a kind of Semen stizolobii capitati albumen.
Background technology
Semen stizolobii capitati has another name called cat bean, Semen Stizolobii Cochinchinensis, white Semen stizolobii capitati, Canis familiaris L. bean, is the seed of pulse family Mucuna plant, for Guangxi characteristic medicine-food two-purpose resource plant.Its original producton location is South Asia, at Southwestern China, also has distribution south China height above sea level 800 ~ 1400m tor, and Guangxi is topmost producing region.Containing natural levodopa in cat bean, there is important using value, in addition, possibly together with substantial amounts of protein and starch.In China, Semen stizolobii capitati is processed into roughage by only indivedual producers, or utilizes its partial starch to be processed into vermicelli, and remainder is abandoned in vain, causes waste and pollutes environment.
In Semen stizolobii capitati, protein is the abundantest, suitable with Food Legume, has higher using value.The wild Semen stizolobii capitati of development and utilization, can improve the economic benefit of flour mill, make rational use of resources, and promotes oil and foodstuffs industrial expansion.But the at present exploitation to Semen stizolobii capitati are concentrated mainly on the extraction of the materials such as levodopa in Semen stizolobii capitati and utilize aspect, the most not about the research of Semen stizolobii capitati protein extraction technique.
Therefore, prior art needs to improve further.
Summary of the invention
In view of the deficiencies in the prior art, the invention reside in the extracting method that a kind of Semen stizolobii capitati albumen is provided, it is intended to solve currently without effective Semen stizolobii capitati protein extraction technique.
Technical scheme is as follows:
Crossing 100 eye mesh screens after being pulverized by Semen stizolobii capitati, the raw material after sieving is the solid-liquid ratio addition NaOH solution of 1: 15-25 in mass ratio, and lixiviating solution is centrifuged, collects supernatant, obtain Semen stizolobii capitati protein solution by stirring and leaching under the conditions of 40-50 DEG C.
The extracting method of described Semen stizolobii capitati albumen, wherein, the concentration of NaOH solution used is 1 × 10-5mol/L-1×10-4mol/L。
The extracting method of described Semen stizolobii capitati albumen, wherein, the concentration of NaOH solution used is 5 × 10-5mol/L。
The extracting method of described Semen stizolobii capitati albumen, wherein, described in sieve after the solid-liquid ratio of raw material and NaOH solution be 1: 20.
The extracting method of described Semen stizolobii capitati albumen, wherein, described extraction is carried out under the conditions of 45 DEG C.
The extracting method of described Semen stizolobii capitati albumen, wherein, described extraction time is 35-45 min.
The extracting method of described Semen stizolobii capitati albumen, wherein, described extraction time is 40 min.
The extracting method of described Semen stizolobii capitati albumen, wherein, Semen stizolobii capitati protein solution extraction obtained is vacuum dried at 40-45 DEG C.
Beneficial effect: the present invention provides the extracting method of a kind of Semen stizolobii capitati albumen, and described extracting method is simple to operate, the yield of Semen stizolobii capitati albumen is high, and albuminous degeneration rate is low, and extracting cycle is short, and efficiency is high, and with low cost, is suitable for carrying out the extensive extraction of Semen stizolobii capitati albumen.
Detailed description of the invention
The present invention provides the extracting method of a kind of Semen stizolobii capitati albumen, and for making the purpose of the present invention, technical scheme and effect clearer, clear and definite, the present invention is described in more detail below.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
The present invention provides the extracting method of a kind of Semen stizolobii capitati albumen, and wherein, described method is:
First 100 eye mesh screens are crossed after being pulverized by Semen stizolobii capitati.After Semen stizolobii capitati is pulverized, through the even particle size that 100 eye mesh screens sieve, grain diameter is little, in subsequent extracted step, contact area with alkali liquor can be greatly improved, accelerate response speed, shorten protein extraction time and efficiency.
Secondly, the raw material after sieving is the solid-liquid ratio addition NaOH solution of 1: 15-25 in mass ratio, stirring and leaching under the conditions of 40-50 DEG C.
From Semen stizolobii capitati, extract the increase of the solid-liquid ratio of protein, accelerate the dissolving of protein in Semen stizolobii capitati, but when solid-liquid ratio is excessive, protein content has declined.It is demonstrated experimentally that when solid-liquid ratio be in mass ratio 1: 15-1: 25 carry out protein extraction time, in Semen stizolobii capitati, the extraction efficiency of protein is high.Preferably, when solid-liquid ratio reaches 1: 20, in lixiviating solution, protein content reaches maximum.
Owing to dilute alkaline soln can accelerate the dissolving extraction of protein, but when NaOH concentration is too high, protein denaturation but can be caused to reduce the extraction ratio of protein, it is demonstrated experimentally that the concentration of NaOH solution used is 1 × 10-5mol/L-1×10-4During mol/L, can effectively extract Semen stizolobii capitati albumen.Preferably, the concentration of NaOH solution is 5 × 10-5During mol/L, in Semen stizolobii capitati lixiviating solution, the extraction ratio of protein is the highest, is 43.5%.
Owing to the raising of alkali liquid temperature can be effectively improved the extraction quantity of protein in Semen stizolobii capitati, under the conditions of 40-50 DEG C, it is most that extraction obtains Semen stizolobii capitati protein content.Preferably, described extraction is carried out under the conditions of 45 DEG C, and the most not only Semen stizolobii capitati protein content is the highest, and albumen deformation is the most less, it is ensured that the highest protein extracting ratio, the most also saves the cost needed for heating.
Owing to extraction time is the longest, protein extraction amount is the most, but protein extraction amount increase is increasingly slower, and easily occurs to infect due to microorganism to make protein extraction liquid occur to go bad.And when extraction time is 35-45 min, can not only be prevented effectively from microorganism infects, and protein extraction amount is high, also saves time cost, it is ensured that extraction efficiency.Preferably, when extraction time is 40 min, and protein extraction efficiency is the highest, and extracted amount is the highest.
Finally, lixiviating solution is centrifuged, collects supernatant, obtain Semen stizolobii capitati protein solution.Wherein, the centrifugal speed of described lixiviating solution is 3500-4500 r/min, and centrifugation time is 10-20min.Under above-mentioned centrifugal speed, can guarantee that in lixiviating solution, Semen stizolobii capitati starch is the most fully deposited in bottom centrifuge tube, advantageously reduced the content of starch impurities in Semen stizolobii capitati albumen, improve purity of protein.Centrifugal 10-20min, can guarantee that and just make the starch in lixiviating solution fully precipitate, also avoided long centrifugal reduction work efficiency.Preferably, when centrifugal speed is 4000 r/min, and centrifugation time is 15min, the protein extraction amount finally given is maximum, and efficiency is the highest.
Semen stizolobii capitati protein solution extraction obtained is vacuum dried at 40-45 DEG C, at this temperature, can guarantee that, on the premise of Semen stizolobii capitati protein never degenerates, Semen stizolobii capitati protein carries out quickly, is dried fully.Preferably, when Semen stizolobii capitati protein extraction obtained is vacuum dried under the conditions of 42 DEG C, Semen stizolobii capitati protein quality is obtained the highest.
For the preparation method of the more detailed description present invention, it is exemplified below several embodiment further description.
Embodiment
1
The extracting method of the Semen stizolobii capitati albumen that the present invention provides is as follows:
100 eye mesh screens are crossed after being pulverized by Semen stizolobii capitati；Raw material after sieving be in mass ratio 1: 20 solid-liquid ratio add concentration be 5 × 10-5The NaOH solution of mol/L, by lixiviating solution is centrifuged after extracting 40min under the conditions of 45 DEG C, and centrifugal speed is 4000 r/min, centrifugation time is 15min, collect supernatant, obtain Semen stizolobii capitati protein solution, finally Semen stizolobii capitati protein supernatant is vacuum dried at 42 DEG C.Test result indicate that, Semen stizolobii capitati Protein Extraction content is 16.25%, and extraction ratio is 64.3%.
Embodiment
2
The extracting method of the Semen stizolobii capitati albumen that the present invention provides is as follows:
100 eye mesh screens are crossed after being pulverized by Semen stizolobii capitati；Raw material after sieving be in mass ratio 1: 15 solid-liquid ratio add concentration be 1 × 10-5The NaOH solution of mol/L, by lixiviating solution is centrifuged after extracting 50min under the conditions of 40 DEG C, and centrifugal speed is 3500 r/min, centrifugation time is 10min, collect supernatant, obtain Semen stizolobii capitati protein solution, finally Semen stizolobii capitati protein supernatant is vacuum dried at 42 DEG C.Test result indicate that, Semen stizolobii capitati Protein Extraction content is 15.50%, and extraction ratio is 61.3%.
Embodiment
3
The extracting method of the Semen stizolobii capitati albumen that the present invention provides is as follows:
100 eye mesh screens are crossed after being pulverized by Semen stizolobii capitati；Raw material after sieving be in mass ratio 1: 25 solid-liquid ratio add concentration be 1 × 10-4The NaOH solution of mol/L, by lixiviating solution is centrifuged after extracting 45min under the conditions of 50 DEG C, and centrifugal speed is 4500 r/min, centrifugation time is 20min, collect supernatant, obtain Semen stizolobii capitati protein solution, finally Semen stizolobii capitati protein supernatant is vacuum dried at 42 DEG C.Test result indicate that, Semen stizolobii capitati Protein Extraction content is 15.83%, and extraction ratio is 62.6%.
The extracting method of the Semen stizolobii capitati albumen that the present invention provides is the most simple to operate, and the yield of Semen stizolobii capitati albumen is high, and albuminous degeneration rate is low, and extracting cycle is short, and efficiency is high, and with low cost, is a kind of extensive method extracted being appropriate to Semen stizolobii capitati albumen.
It should be appreciated that the application of the present invention is not limited to above-mentioned citing, for those of ordinary skills, can be improved according to the above description or convert, all these modifications and variations all should belong to the protection domain of claims of the present invention.
Claims (9)
1. the extracting method of a Semen stizolobii capitati albumen, it is characterised in that described method is:
100 eye mesh screens are crossed after being pulverized by Semen stizolobii capitati；Raw material after sieving is the solid-liquid ratio addition NaOH solution of 1: 15-25 in mass ratio, and lixiviating solution is centrifuged, collects supernatant, obtain Semen stizolobii capitati protein solution by stirring and leaching under the conditions of 40-50 DEG C.
The extracting method of Semen stizolobii capitati albumen the most according to claim 1, it is characterised in that the concentration of NaOH solution used is 1 × 10-5mol/L-1×10-4mol/L。
The extracting method of Semen stizolobii capitati albumen the most according to claim 2, it is characterised in that the concentration of NaOH solution used is 5 × 10-5mol/L。
The extracting method of Semen stizolobii capitati albumen the most according to claim 1, it is characterised in that described in sieve after the solid-liquid ratio of raw material and NaOH solution be 1: 20.
The extracting method of Semen stizolobii capitati starch the most according to claim 1, it is characterised in that the centrifugal speed of described lixiviating solution is 3500
-4500 r/min, centrifugation time is 10-20min.
The extracting method of Semen stizolobii capitati albumen the most according to claim 1, it is characterised in that described extraction is carried out under the conditions of 45 DEG C.
The extracting method of Semen stizolobii capitati albumen the most according to claim 1, it is characterised in that described extraction time is 35-45 min.
The extracting method of Semen stizolobii capitati albumen the most according to claim 7, it is characterised in that described extraction time is 40 min.
The extracting method of Semen stizolobii capitati albumen the most according to claim 1, it is characterised in that Semen stizolobii capitati protein solution extraction obtained is vacuum dried at 40-45 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610357089.6A CN105777861A (en) | 2016-05-26 | 2016-05-26 | Cowage protein extraction method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610357089.6A CN105777861A (en) | 2016-05-26 | 2016-05-26 | Cowage protein extraction method |
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| CN105777861A true CN105777861A (en) | 2016-07-20 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109224510A (en) * | 2018-11-20 | 2019-01-18 | 那坡康正天然植物提取有限责任公司 | A kind of extractor producing multitude's beans extract |
| CN116711805A (en) * | 2023-06-06 | 2023-09-08 | 安徽兴宙医药有限公司 | Preparation method of rice protein |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1045969A (en) * | 1989-03-28 | 1990-10-10 | 张宪德 | Method with cat beans or multitude's beans production levodopa |
| CN1429838A (en) * | 2001-12-30 | 2003-07-16 | 申成果 | Technology of extracting soybean protein isolate |
-
2016
- 2016-05-26 CN CN201610357089.6A patent/CN105777861A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1045969A (en) * | 1989-03-28 | 1990-10-10 | 张宪德 | Method with cat beans or multitude's beans production levodopa |
| CN1429838A (en) * | 2001-12-30 | 2003-07-16 | 申成果 | Technology of extracting soybean protein isolate |
Non-Patent Citations (1)
| Title |
|---|
| ADEBOWALE, KO等: "Foaming, gelation and electrophoretic characteristics of mucuna bean (Mucuna pruriens) protein concentrates", 《FOOD CHEMISTRY》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109224510A (en) * | 2018-11-20 | 2019-01-18 | 那坡康正天然植物提取有限责任公司 | A kind of extractor producing multitude's beans extract |
| CN116711805A (en) * | 2023-06-06 | 2023-09-08 | 安徽兴宙医药有限公司 | Preparation method of rice protein |
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Application publication date: 20160720 |