CN116660001A - Tissue dehydration positioning embedding assembly and application method thereof - Google Patents
Tissue dehydration positioning embedding assembly and application method thereof Download PDFInfo
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- CN116660001A CN116660001A CN202310435907.XA CN202310435907A CN116660001A CN 116660001 A CN116660001 A CN 116660001A CN 202310435907 A CN202310435907 A CN 202310435907A CN 116660001 A CN116660001 A CN 116660001A
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- 230000018044 dehydration Effects 0.000 title claims abstract description 27
- 238000006297 dehydration reaction Methods 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 22
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 63
- 230000009975 flexible effect Effects 0.000 claims abstract description 30
- 229940057995 liquid paraffin Drugs 0.000 claims abstract description 16
- 239000000463 material Substances 0.000 claims description 30
- 239000007788 liquid Substances 0.000 claims description 12
- 238000001816 cooling Methods 0.000 claims description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 239000000741 silica gel Substances 0.000 claims description 4
- 229910002027 silica gel Inorganic materials 0.000 claims description 4
- 229920002457 flexible plastic Polymers 0.000 claims description 3
- 239000004816 latex Substances 0.000 claims description 3
- 229920000126 latex Polymers 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 2
- 230000007306 turnover Effects 0.000 abstract description 6
- 230000008569 process Effects 0.000 description 13
- 230000000694 effects Effects 0.000 description 9
- 230000000903 blocking effect Effects 0.000 description 7
- 230000004308 accommodation Effects 0.000 description 6
- 238000001746 injection moulding Methods 0.000 description 4
- 238000001514 detection method Methods 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 230000003014 reinforcing effect Effects 0.000 description 2
- RRHGJUQNOFWUDK-UHFFFAOYSA-N Isoprene Chemical compound CC(=C)C=C RRHGJUQNOFWUDK-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011127 biaxially oriented polypropylene Substances 0.000 description 1
- 229920006378 biaxially oriented polypropylene Polymers 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 239000001993 wax Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- Life Sciences & Earth Sciences (AREA)
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- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The application relates to the technical field of biological tissue sample treatment, and particularly discloses a tissue dehydration positioning embedding assembly and a use method thereof. The tissue dehydration positioning embedding assembly comprises a sample accommodating box, a reagent accommodating box and an embedding bracket, wherein the sample accommodating box is arranged in the reagent accommodating box, and the embedding bracket is arranged in the reagent accommodating box and is positioned above the sample accommodating box; the device also comprises a positioning piece with flexible texture, wherein the positioning piece is positioned between the embedding bracket and the sample placing box; the locating piece includes the locating strip, and after the tissue dehydration location embedding subassembly was assembled, the locating strip was placed the bottom of box towards the sample. According to the application, the positioning piece with flexible texture is matched with the embedding bracket, so that the biological tissue sample is positioned from top to bottom, and when the reagent or the liquid paraffin is added, the tissue sample is not easy to float, turn over, curl and the like, so that the formed embedded tissue sample is in a relatively flat and reasonable state, and the subsequent sections are relatively sequential and observed more accurately.
Description
Technical Field
The application relates to the technical field of biological tissue sample treatment, in particular to a tissue dehydration positioning embedding assembly and a use method thereof.
Background
The dehydration embedding component generally comprises a sample placement box, a reagent containing box and an embedding bracket, wherein the sample placement box is placed in the reagent containing box and used for placing a biological tissue sample, and the embedding bracket is placed in the sample placement box and is provided with a gap with the sample placement box.
Because dehydration embedding subassembly belongs to the consumptive material of detection field, generally all is batch production, and in order to be applicable to biological tissue sample as far as possible, and be difficult for making the tissue sample take place to warp because of being extruded in embedding process, consequently, the clearance between embedding support and the sample placement box is usually great.
However, for biological tissue samples with lighter or thinner texture, in the process of adding reagents or liquid paraffin to the assembled embedding assembly, as the liquid level of the reagents or liquid paraffin continuously rises, the tissue samples float or turn over to different extents, especially when the tissue samples turn over, curl or stand up, the position or state of the tissue samples during embedding is easily unreasonable, and after the liquid paraffin is cooled and molded, the tissue samples still remain unreasonable in position or state, and finally the subsequent operations of slicing, observing and the like are affected.
Disclosure of Invention
In order to solve the problems that turnover, curling or standing and the like can occur in the process of embedding a tissue sample, the application provides a tissue dehydration positioning embedding assembly and a use method thereof.
In a first aspect, the application provides a tissue dehydration positioning embedding assembly, which comprises the following specific technical scheme:
the tissue dehydration positioning embedding assembly comprises a sample placement box, a reagent accommodation box and an embedding bracket, wherein the sample placement box is arranged in the reagent accommodation box, and the embedding bracket is arranged in the reagent accommodation box and is positioned above the sample placement box; the device also comprises a positioning piece with flexible texture, wherein the positioning piece is positioned between the embedding bracket and the sample placing box; the locating piece comprises a locating strip, and when the tissue dehydration locating embedding component is assembled, the locating strip faces to the bottom of the sample placing box.
In the application, a positioning piece is arranged between the embedding bracket and the sample placing box, and the biological tissue sample is stabilized by the positioning piece. The positioning piece is soft in texture and has certain toughness, so that the positioning effect on a tissue sample can be achieved, the biological tissue sample is not easy to damage, and the slicing effect is not affected.
The number of the positioning strips is not limited, and the positioning strips can be one or two or more; the shape of the positioning strip is not limited, and the positioning strip can be columnar, table-shaped and the like; the length of the positioning strip is not limited, if the length is too long, the bending can be realized due to the flexible effect of the positioning strip, and the tissue sample can be positioned and blocked, but the state of the tissue sample is not damaged; if the length is shorter, the phenomenon that the biological tissue sample overturns, curls or stands upright can be prevented between the embedding bracket and the sample placing box, and the phenomenon that the tissue sample overturns or floats can be reduced after the reagent or the liquid paraffin is added.
One end of the positioning strip faces to the bottom of the sample placement box, contacts with the biological tissue sample, and the other end of the positioning strip is in contact with or fixed with the embedding bracket, so that a certain positioning and blocking effect is generated on the biological tissue sample, the contact and coating effects of the added reagent or liquid paraffin and the tissue sample are good, and great help is provided for the follow-up improvement of the accuracy of slice observation.
Further, the locating strip is fixed at the bottom of the embedding bracket.
Through above-mentioned technical scheme, be favorable to improving the convenience of operation, when embedding support was placed in the reagent and hold the box, the locating strip also lies in the sample in step and places the box and contact with biological tissue sample.
Further, the positioning piece further comprises a carrier, and the carrier is fixedly connected with the positioning strip.
The carrier and the locating strip are fixedly connected to form a whole, so that the overall stability of the locating piece is improved, the locating piece can be separated from the embedded support for use or can be fixedly used with the embedded support, and the locating piece can play a good role in locating biological tissue samples.
Further, the carrier is fixedly connected with the bottom of the embedding bracket.
Through the technical scheme, the convenience of operation is further improved.
Further, the carrier is at least one of a strip shape, a frame shape and a sheet shape with through holes.
The shape of the carrier adopted in the application is not limited, and the carrier can be strip-shaped, frame-shaped and sheet-shaped with through holes, and the through holes on the embedding bracket are not blocked except the shape.
Further, the length of the positioning strip is not greater than the interval between the bottom of the embedding bracket and the bottom of the sample placing box when the embedding bracket is placed in the sample placing box; and a plurality of positioning strips are arranged inwards from the periphery, and the lengths of the positioning strips are gradually reduced to form a concave positioning area.
Through the technical scheme, the length of the positioning strip is set to be moderate, so that a comprehensive limiting effect can be formed on the tissue sample from top to bottom, excessive propping can not be caused on the tissue sample, the tissue sample can be kept in a relatively original state, and the accuracy of slice detection can be improved. At the same time, the concave positioning area is gradually presented to the middle, and the tissue sample is well contained while being sufficiently positioned.
Further, the spacing between the adjacent positioning strips is 0.3-1.5mm; preferably, the spacing between adjacent positioning strips is 0.3mm;
the center distance between the adjacent positioning strips is not less than 0.5mm; preferably, the center distance between adjacent positioning strips is 0.8mm.
Through above-mentioned technical scheme, set up the interval between the location strip, make and have certain gap between the location strip, in addition, inject the thickness of location strip, can play material-saving's effect, and leave the gap between the location strip, still contact surface between the tissue sample is less, can not only add the in-process of reagent, let the tissue sample more fully contact with reagent, thereby reach effects such as better dehydration, can also reach better observation effect after the section, be difficult for influencing the observation because the contact of location strip and tissue sample is too much.
Further, the positioning piece is made of flexible materials; the flexible material is at least one of liquid silica gel, flexible plastic and latex; the Shore hardness of the positioning piece is 5-60HA.
The shore hardness of the flexible material adopted in the application is 5-60HA, such as liquid silica gel, flexible plastic, latex and the like, and the flexible material can be selected as the flexible material as long as the shore hardness range is met. The texture of the flexible material is soft and has certain toughness, so that the flexible material can play a good role in positioning and blocking a tissue sample, reduce the phenomena of overturning, curling and the like, and can not damage the tissue sample. If the shore hardness is higher than 60HA, the phenomenon of damage to part of biological tissue samples is easy to occur in the operation process, so that the shore hardness of the flexible material adopted by the positioning piece is reasonably controlled to be 5-60HA. The flexible materials used in the present application include, but are not limited to, the materials described above.
Further, the preparation of the positioning piece comprises the following steps:
injecting the melted flexible material into a mold of the positioning piece, cooling and forming, and removing the mold of the positioning piece to obtain the positioning piece;
the mould of setting element is connected with the bottom of embedding support.
In the application, the melted flexible material is injected into the mould for molding, so that the positioning pieces with various shapes can be obtained, and the positioning pieces, the embedding bracket and the sample placing box are all in a separated state, namely, the positioning pieces can only contain positioning strips; when the mould of locating part and the bottom fixed connection of embedding support, pour into the flexible material after the melting into the mould again and take shape, this kind of mode can make the locating part that obtains after the cooling form with embedding support to be connected, and it is more convenient when assembling the tissue dehydration location embedding subassembly, and can play better locate action to biological tissue sample. In this case, the positioning member may also include a carrier and a positioning bar.
In a second aspect, the application provides a method for using a tissue dehydration positioning embedding component, which comprises the following steps:
the application method of the tissue dehydration positioning embedding component comprises the following steps:
placing a sample placing box in a reagent containing box, placing a biological tissue sample at the bottom of the sample placing box, placing a positioning piece on the biological tissue sample, and placing an embedding bracket on the reagent containing box and above the sample placing box;
injecting a reagent, immersing the reagent at the bottom of the embedding bracket, taking out the reagent from a liquid taking tank body of the reagent accommodating box, and repeating the operation;
injecting liquid paraffin, immersing the liquid paraffin at the bottom of the embedding bracket, cooling and solidifying, and taking out the embedding bracket, thereby obtaining a tissue sample embedded on the embedding bracket;
in the first step, the sample placement box is placed in the reagent containing box, the biological tissue sample is placed at the bottom of the sample placement box, and then the embedding bracket fixed with the positioning piece is placed on the reagent containing box and above the sample placement box.
When the locating piece is separated from the embedding bracket, the locating piece is required to be placed on the biological tissue sample, then the embedding bracket is placed on the reagent containing box, at the moment, one end of the locating piece is enabled to be contacted with the bottom of the embedding bracket, and the other end of the locating piece is enabled to be contacted with the biological tissue sample, and the purposes of locating and blocking the biological tissue sample are achieved in the process of injecting the reagent.
When the locating piece is fixed with the embedding bracket, the locating piece can be contacted with the biological tissue sample at one end far away from the embedding bracket and form better locating and blocking effects as long as the whole embedding bracket fixed with the locating piece is clamped and fixed in the reagent containing box and is positioned above the sample placing box.
In summary, the application has the following beneficial effects:
1. the positioning piece with flexible texture is matched with the embedding bracket, so that the biological tissue sample placed in the sample placement box is positioned from top to bottom, and when reagents or liquid paraffin are added, the biological tissue sample is not easy to float, turn over, curl and the like, so that the formed embedded biological tissue sample is in a relatively flat and reasonable state, and subsequent sections are relatively sequential and observed more accurately.
2. The locating piece is made of flexible materials, the Shore hardness of the locating piece is in a reasonable range, such as liquid silica gel and the like, the biological tissue sample is not easy to damage, the later stage is not easy to block when the embedded biological tissue sample is sliced, and the biological tissue sample can be sliced smoothly.
Drawings
FIG. 1 is a schematic view of the overall structure of a tissue dewatering positioning embedding assembly in embodiment 1;
FIG. 2 is a schematic diagram of the exploded construction of the tissue dewatering positioning embedding assembly of example 1;
FIG. 3 is a schematic view showing the structure of an embedding stent in example 1;
FIG. 4 is a schematic view of the structure of the positioning bar in embodiment 1;
FIG. 5 is a schematic view of the structure of the positioning bar in embodiment 2;
fig. 6 is a schematic view showing the overall structure of the positioning member in embodiment 4.
1. A sample placement cartridge; 11. a placement area; 12. a through hole; 2. a reagent accommodating box; 21. a housing case; 22. an extension end; 23. a liquid taking tank; 3. embedding a bracket; 31. a frame body; 32. a support plate; 33. a connecting plate; 34. arc-shaped notch; 35. embedding holes; 36. a reinforcing column; 4. a positioning piece; 41. a positioning strip; 42. a carrier; 5. and positioning the area.
Detailed Description
Examples
Example 1: the tissue dehydration positioning embedding assembly comprises a sample placement box 1, a reagent accommodation box 2 and an embedding bracket 3, wherein the sample placement box 1 is clamped in the reagent accommodation box 2, and the embedding bracket 3 is arranged in the reagent accommodation box 2 and is positioned above the sample placement box 1.
As shown in fig. 2, the reagent accommodating box 2 includes an accommodating box body 21 and an extending end 22 integrally formed with the accommodating box body 21, and a liquid taking groove 23 communicated with the accommodating box body 21 is formed in the extending end 22.
The middle position of the bottom of the sample placement box 1 is provided with a placement area 11 for placing a biological tissue sample in an outward protruding mode, and 12 through holes 12 are formed in the placement area 11, and in the process of adding a reagent, the reagent leaks into the reagent accommodating box 2 through the through holes 12, and the added reagent can be gradually lifted up and immersed through the biological tissue sample through the through holes 12, so that the reagent is fully contacted with the tissue sample.
The embedding bracket 3 comprises a surrounding frame body 31 and a supporting plate 32, wherein the frame body 31 and the supporting plate 32 are connected through a connecting plate 33, an arc-shaped notch 34 is formed in one side of the frame body 31, and a plurality of strip-shaped embedding holes 35 are formed in the supporting plate 32. And when the sample placing cartridge 1, the reagent holding cartridge 2 and the embedding bracket 3 are assembled, the notch is positioned right above the liquid taking groove 23.
As shown in fig. 3, a plurality of columnar positioning strips 41 are fixedly connected to the bottom of the sample placement box 1. And the fixing manner of the positioning strip 41 is as follows:
the mold is fixed at the bottom of the support plate 32 embedding the support 3, the molten flexible material is injected into the mold, and the mold is cooled and formed, so that a positioning area 5 (combining fig. 2 and 3) composed of a plurality of columnar positioning strips 41 with the same length is formed at the middle position of the bottom of the support plate 32. Referring to fig. 4, the positioning strip 41 of the peripheral ring is divided into two sections with different thicknesses, and the diameter of the side connected with the embedding bracket 3 is larger than the diameter of the side away from the embedding bracket 3; the diameter of the positioning strip 41 inside the peripheral ring is the same as the diameter of the side facing away from the embedding support 3 and the length is the same as the length of the side connected to the embedding support 3. The length of the positioning strip 41 gradually decreases from the outer periphery to the inner periphery, so that the formed positioning area 5 takes a concave shape from the outside to the inside. On the side facing away from the embedding support 3, the spacing between adjacent positioning strips 41 is 0.3mm, the center distance between adjacent positioning strips 41 is 0.8mm, the size specification of the positioning strips is consistent with that of the placement area 11, and the formed positioning strips 41 are flexible in texture.
The tissue dehydration positioning embedding assembly has the following operation steps in the use process:
firstly, placing a sample placing box 1 in a reagent containing box 2, enabling the outer wall of the sample placing box 1 to be abutted against the inner wall of the reagent containing box 2, placing a biological tissue sample in a placing area 11 in the sample placing box 1, placing an embedding bracket 3 fixed with a positioning strip 41 on the reagent containing box 2, namely, erecting a frame 31 on the upper edge of the reagent containing box 2, enabling an arc-shaped notch 34 to be positioned right above a liquid taking groove 23 on the reagent containing box 2, enabling the positioning strip 41 to face right above the placing area 11, and enabling the length of the positioning strip 41 to be equal to the interval between a supporting plate 32 and the bottom of the sample placing box 1;
injecting a reagent, immersing the reagent in the bottom of the embedding bracket 3, sucking the reagent from the liquid taking groove 23 of the reagent containing box 2, and repeating the operation until the tissue sample is dehydrated completely;
and thirdly, injecting liquid paraffin, enabling the liquid paraffin to permeate the bottom of the embedded bracket 3, cooling and solidifying, and taking out the embedded bracket 3, thereby obtaining the tissue sample embedded on the embedded bracket 3.
The reagent holding box 2 and the embedding bracket 3 in the embodiment are made of plastic materials, the sample placing box 1 is made of stainless steel materials, and the purpose of the arrangement is to enable the cooled and solidified wax block to be separated from the sample placing box 1 along with the embedding bracket 3. The flexible material in this embodiment is polyurethane E255.
The positioning strips 41 are composed of two sections with different thicknesses, one thin end is in contact with the biological tissue sample, the contact area of the thin end and the biological tissue sample is small, gaps with different sizes exist between the adjacent positioning strips 41, and when reagents or liquid paraffin are injected, the biological tissue sample is immersed in the gaps, so that the biological tissue sample is dehydrated or coated more fully, and the accuracy of subsequent detection is improved.
Example 2: a tissue dewatering positioning embedding assembly differing from example 1 in that: as shown in fig. 5, the positioning member 4 is a positioning strip 41, which has a thick end and a thin end, and is integrally formed in a stepped shape. During the assembly of the tissue dehydration positioning embedding assembly, the positioning strip 41 is not fixed with the embedding bracket 3, and the thin end of the positioning strip 41 contacts the tissue sample, and the thick end contacts the bottom of the embedding bracket 3. The positioning strip 41 is manufactured by injection molding.
In this way, the positioning bar 41 is more easily obtained, but in the assembly process, the positioning bar 41 is less easily placed between the sample placement cartridge 1 and the embedding bracket 3 than in the embodiment 1, but the same effect as in the embodiment 1 can be achieved.
Example 3: a tissue dewatering positioning embedding assembly differing from example 1 in that: the positioning strip 41 has a columnar shape with uniform thickness.
Although the shape of the positioning strip 41 is different from that of the embodiment 1, the requirement on the die is lower, and the obtained positioning strip 41 still has soft texture, can generate better blocking and positioning effects on the biological tissue sample, and can also keep the original shape of the biological tissue sample.
Example 4: a tissue dewatering positioning embedding assembly differing from example 1 in that: as shown in fig. 6, the positioning member 4 is composed of a carrier 42 and a positioning bar 41, the carrier 42 is in the form of a sheet with the through holes 12, the positioning bar 41 is provided at a position where the carrier 42 is not provided with the through holes 12, and the carrier 42 and the positioning bar 41 are obtained by injection molding. In the process of assembling the tissue dehydration positioning embedding assembly, the positioning piece 4 is not fixedly connected with the bottom of the embedding bracket 3.
The carrier 42 is added, and the carrier 42 and the positioning strips 41 are formed by injection molding, so that the production is convenient, the overall stability of the positioning piece 4 is improved, and the positioning piece is easier to place than the positioning strips 41 in the embodiment 2 in the process of assembling the tissue dehydration positioning embedding component.
Example 5: a tissue dewatering positioning embedding assembly differing from example 4 in that: the positioning piece 4 consists of a carrier 42 and a positioning strip 41, and the carrier 42 is fixedly connected with the bottom of the embedding bracket 3.
The carrier 42 in the positioning piece 4 is directly fixedly connected with the embedding bracket 3, so that the tissue dehydration positioning embedding assembly is more convenient to assemble, and the blocking and positioning of the biological tissue sample are more stable.
Example 6: a tissue dewatering positioning embedding assembly differing from example 5 in that: the carrier 42 is frame-shaped, and the positioning strips 41 are arranged on the frame strips, and the positioning strips 41 do not block the through holes 12 in the process of assembling the tissue dehydration positioning embedding assembly.
The carrier 42 in example 6 has only one frame strip, and the number of the positioning strips 41 arranged on the frame strip is smaller than that of the positioning strips 41 in example 5, but the surrounding ring can be formed, the biological tissue sample can be surrounded and positioned, even the edge of the biological tissue sample is pressed, and the phenomenon that the sample curls is reduced as much as possible. And the carrier 42 of such a shape can save the practicality of flexible material, is favorable to reduce cost.
Example 7: a tissue dewatering positioning embedding assembly differing from example 5 in that: the carrier 42 is a plurality of strips, the positioning strips 41 are integrally connected with the carrier 42, and the positioning strips 41 do not block the through holes 12 in the process of assembling the tissue dehydration positioning embedding component.
The strip-shaped carrier 42 can be completely fixed with the bottom of the embedding bracket 3, and the strip-shaped carrier 42 and the positioning strips 41 on the carrier 42 do not block the through holes 12 at all, so that reagents or liquid paraffin can be injected more smoothly and can be contacted with a biological tissue sample. The mold used for the strip-shaped carrier 42 is simpler, and compared with the shape of the carrier 42 in the embodiment 5, the strip-shaped carrier 42 in the embodiment saves more cost of flexible materials; the positioning member 4 can be optionally adjusted in position at the bottom of the embedding assembly to form a specific size or shape, so that the formed positioning member 4 can be used for adapting to more biological tissue samples with different shapes.
Example 8: a tissue dewatering positioning embedding assembly differing from example 7 in that: the length of the positioning strip 41 is four fifths of the distance between the bottom of the embedding bracket 3 and the bottom of the sample placement box 1 when the embedding bracket 3 is placed in the sample placement box 1, and after the tissue dehydration positioning embedding assembly is assembled, a small gap may be left between the end of the positioning strip 41 and the tissue sample, and contact may also occur between the positioning strip and the tissue sample, specifically according to the situation of the tissue sample actually placed.
The positioning strips 41 are uniformly set to have the above length, so that a certain space can be reserved for the tissue sample, and the original state can be maintained as much as possible, but the possible gap between the positioning strips 41 and the tissue sample can not cause the tissue sample to turn over, curl or stand up.
Example 9: a tissue dewatering positioning embedding assembly differing from example 7 in that: the positioning strips 41 are columnar in shape, and the spacing between adjacent positioning strips 41 is 0.8mm.
Example 10: a tissue dewatering positioning embedding assembly differing from example 9 in that: the center-to-center distance between adjacent ones of the positioning strips 41 is 1.0mm.
Example 11: a tissue dewatering positioning embedding assembly differing from example 7 in that: the positioning strips 41 are columnar in shape, and the spacing between adjacent positioning strips 41 is 1.5mm.
Example 12: a tissue dewatering positioning embedding assembly differing from example 11 in that: the center-to-center distance between adjacent ones of the positioning bars 41 is 2.4mm.
Example 13: a tissue dewatering positioning embedding assembly differing from example 7 in that: the positioning strips 41 are columnar in shape, and the spacing between adjacent positioning strips 41 is 0.3mm.
Example 14: a tissue dewatering positioning embedding assembly differing from example 13 in that: the center-to-center distance between adjacent ones of the positioning strips 41 is 0.5mm.
In examples 9, 11 and 13, only the spacing at the edges of adjacent positioning strips is defined, i.e. the gaps between adjacent positioning strips are defined; in embodiments 10, 12, and 14, not only the spacing between the edges of adjacent positioning strips, but also the center distance between the positioning strips, that is, the thickness and the gap size of the adjacent positioning strips, are limited, and compared with embodiments 9, 11, and 13, embodiments 10, 12, and 14 still have the effect of blocking and positioning the biological tissue sample, and can save more cost.
Example 15: a tissue dewatering positioning embedding assembly differing from example 7 in that: the flexible material is BOPP.
Example 16: a tissue dewatering positioning embedding assembly differing from example 7 in that: the flexible material is a CPP.
Example 17: a tissue dewatering positioning embedding assembly differing from example 1 in that: the flexible material is natural latex.
The frame 31 of the embedding bracket 3 in all the above embodiments may further be provided with a plurality of reinforcing columns 36 (refer to fig. 2) for increasing the supporting strength of the embedding bracket 3.
In the embodiment where the positioning member 4 is fixedly connected with the embedding support 3, the material of the positioning member 4 is melted first and then injection molded through a mold, and the melting point of the material of the positioning member 4 is lower than that of the material of the embedding support 3, so that the phenomenon that the junction between the embedding support 3 and the positioning member 4 is locally melted is not easy to occur in the injection molding process.
Comparative example
Comparative example 1: a tissue dewatering positioning embedding assembly differs from embodiment 1 in that the positioning member 4 is not included.
In the use process, the phenomenon of overturning, standing or curling of the biological tissue sample is easy to occur along with the injection of the reagent or the liquid paraffin.
The present embodiment is only for explanation of the present application and is not to be construed as limiting the present application, and modifications to the present embodiment, which may not creatively contribute to the present application as required by those skilled in the art after reading the present specification, are all protected by patent laws within the scope of claims of the present application.
Claims (10)
1. The tissue dehydration positioning embedding assembly comprises a sample placement box (1), a reagent containing box (2) and an embedding bracket (3), wherein the sample placement box (1) is arranged in the reagent containing box (2), and the embedding bracket (3) is arranged in the reagent containing box and is positioned above the sample placement box (1); the device is characterized by further comprising a positioning piece (4) with flexible texture, wherein the positioning piece (4) is positioned between the embedding bracket (3) and the sample placing box (1); the positioning piece (4) comprises a positioning strip (41), and when the tissue dehydration positioning embedding assembly is assembled, the positioning strip (41) faces to the bottom of the sample placement box (1).
2. A tissue dewatering positioning embedding assembly as claimed in claim 1, characterized in that the positioning strip (41) is fixed to the bottom of the embedding bracket (3).
3. A tissue dewatering positioning embedding assembly as claimed in claim 1, wherein the positioning member (4) further comprises a carrier (42), the carrier (42) being fixedly connected with the positioning strip (41).
4. A tissue dewatering positioning embedding assembly as claimed in claim 3, wherein the carrier (42) is fixedly connected to the bottom of the embedding support (3).
5. A tissue dewatering positioning embedding assembly as claimed in claim 3, wherein said carrier (42) is at least one of strip-like, frame-like, sheet-like with through holes (12).
6. A tissue dewatering positioning embedding assembly as claimed in claim 1, wherein the length of the positioning strip (41) is not greater than the distance between the bottom of the embedding rack (3) and the bottom of the sample placement box (1) when the embedding rack (3) is placed in the sample placement box (1); the lengths of the positioning strips (41) are gradually reduced from the periphery to the inside, so that a concave positioning area (5) is formed.
7. A tissue dewatering positioning embedding assembly as claimed in claim 1, wherein the spacing between adjacent positioning strips (41) is 0.3-1.5mm; preferably, the spacing between adjacent positioning strips (41) is 0.3mm;
the center distance between the adjacent positioning strips (41) is not less than 0.5mm; preferably, the center-to-center distance between adjacent positioning strips (41) is 0.8mm.
8. A tissue dewatering positioning embedding assembly as claimed in claim 1, wherein the positioning member (4) is of a flexible material; the flexible material is at least one of liquid silica gel, flexible plastic and latex; the Shore hardness of the positioning piece (4) is 5-60HA.
9. A tissue dewatering positioning embedding assembly as claimed in any of claims 1-8, wherein the preparation of said positioning member (4) comprises the steps of:
injecting the melted flexible material into a mould of the positioning piece (4), cooling and forming, and removing the mould of the positioning piece (4) to obtain the positioning piece (4);
the die of the locating piece (4) is connected with the bottom of the embedding bracket (3).
10. A method of using a tissue dewatering positioning embedding assembly as claimed in any of claims 1 to 9, and including the steps of:
firstly, placing a sample placing box (1) in a reagent accommodating box (2), placing a biological tissue sample at the bottom of the sample placing box (1), placing a positioning piece (4) on the biological tissue sample, and then placing an embedding bracket (3) on the reagent accommodating box (2) and above the sample placing box (1);
injecting a reagent, immersing the reagent in the bottom of the embedding bracket (3), taking the reagent out of a liquid taking groove (23) of the reagent accommodating box (2), and repeating the operation;
injecting liquid paraffin, immersing the liquid paraffin at the bottom of the embedding bracket (3), cooling and solidifying, and taking out the embedding bracket (3) to obtain a tissue sample embedded on the embedding bracket (3);
in the first step, a sample placement box (1) is placed in a reagent containing box (2), a biological tissue sample is placed at the bottom of the sample placement box (1), and an embedding bracket (3) fixed with a positioning piece (4) is placed on the reagent containing box (2) and is positioned above the sample placement box (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310435907.XA CN116660001A (en) | 2023-04-19 | 2023-04-19 | Tissue dehydration positioning embedding assembly and application method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310435907.XA CN116660001A (en) | 2023-04-19 | 2023-04-19 | Tissue dehydration positioning embedding assembly and application method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116660001A true CN116660001A (en) | 2023-08-29 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310435907.XA Pending CN116660001A (en) | 2023-04-19 | 2023-04-19 | Tissue dehydration positioning embedding assembly and application method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116660001A (en) |
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2023
- 2023-04-19 CN CN202310435907.XA patent/CN116660001A/en active Pending
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