CN116590190A - 嗜碱盐单胞菌lh-b.0040及其微生物菌剂与用途 - Google Patents
嗜碱盐单胞菌lh-b.0040及其微生物菌剂与用途 Download PDFInfo
- Publication number
- CN116590190A CN116590190A CN202310620931.0A CN202310620931A CN116590190A CN 116590190 A CN116590190 A CN 116590190A CN 202310620931 A CN202310620931 A CN 202310620931A CN 116590190 A CN116590190 A CN 116590190A
- Authority
- CN
- China
- Prior art keywords
- strain
- halophila
- denitrification
- salt
- microbial agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000544058 Halophila Species 0.000 title claims abstract description 30
- 230000000813 microbial effect Effects 0.000 title claims description 29
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 28
- 239000002351 wastewater Substances 0.000 claims abstract description 18
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 14
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 11
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 6
- 229910002651 NO3 Inorganic materials 0.000 claims abstract description 5
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims abstract description 5
- 238000004321 preservation Methods 0.000 claims abstract description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 24
- 230000001651 autotrophic effect Effects 0.000 claims description 17
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 claims description 14
- 238000000855 fermentation Methods 0.000 claims description 12
- 230000004151 fermentation Effects 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 10
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 8
- 238000000034 method Methods 0.000 abstract description 8
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 abstract description 4
- 238000009825 accumulation Methods 0.000 abstract description 2
- 239000005416 organic matter Substances 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 14
- 150000003839 salts Chemical class 0.000 description 12
- 241000894006 Bacteria Species 0.000 description 11
- 241000432381 Halomonas alkaliphila Species 0.000 description 11
- 230000001580 bacterial effect Effects 0.000 description 9
- 239000000243 solution Substances 0.000 description 8
- 238000004065 wastewater treatment Methods 0.000 description 7
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- 239000000843 powder Substances 0.000 description 6
- NIFIFKQPDTWWGU-UHFFFAOYSA-N pyrite Chemical compound [Fe+2].[S-][S-] NIFIFKQPDTWWGU-UHFFFAOYSA-N 0.000 description 6
- 239000011028 pyrite Substances 0.000 description 6
- 229910052683 pyrite Inorganic materials 0.000 description 6
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 description 6
- 235000019345 sodium thiosulphate Nutrition 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 5
- 239000002054 inoculum Substances 0.000 description 5
- 239000010865 sewage Substances 0.000 description 5
- 230000015556 catabolic process Effects 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- 238000006731 degradation reaction Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000000877 morphologic effect Effects 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 235000003891 ferrous sulphate Nutrition 0.000 description 3
- 239000011790 ferrous sulphate Substances 0.000 description 3
- 230000015784 hyperosmotic salinity response Effects 0.000 description 3
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 3
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 238000003794 Gram staining Methods 0.000 description 2
- 241000206596 Halomonas Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 239000000370 acceptor Substances 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 238000002856 computational phylogenetic analysis Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000009629 microbiological culture Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000003204 osmotic effect Effects 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 229920001021 polysulfide Polymers 0.000 description 2
- 239000005077 polysulfide Substances 0.000 description 2
- 150000008117 polysulfides Polymers 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 229910021646 siderite Inorganic materials 0.000 description 2
- 239000010802 sludge Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- PLXMOAALOJOTIY-FPTXNFDTSA-N Aesculin Natural products OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)[C@H]1Oc2cc3C=CC(=O)Oc3cc2O PLXMOAALOJOTIY-FPTXNFDTSA-N 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- WNBCMONIPIJTSB-BGNCJLHMSA-N Cichoriin Natural products O([C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1)c1c(O)cc2c(OC(=O)C=C2)c1 WNBCMONIPIJTSB-BGNCJLHMSA-N 0.000 description 1
- 229910021591 Copper(I) chloride Inorganic materials 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000033558 biomineral tissue development Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 238000004939 coking Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- OXBLHERUFWYNTN-UHFFFAOYSA-M copper(I) chloride Chemical compound [Cu]Cl OXBLHERUFWYNTN-UHFFFAOYSA-M 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- XHCADAYNFIFUHF-TVKJYDDYSA-N esculin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC(C(=C1)O)=CC2=C1OC(=O)C=C2 XHCADAYNFIFUHF-TVKJYDDYSA-N 0.000 description 1
- 229940093496 esculin Drugs 0.000 description 1
- AWRMZKLXZLNBBK-UHFFFAOYSA-N esculin Natural products OC1OC(COc2cc3C=CC(=O)Oc3cc2O)C(O)C(O)C1O AWRMZKLXZLNBBK-UHFFFAOYSA-N 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 230000004660 morphological change Effects 0.000 description 1
- 230000001546 nitrifying effect Effects 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000013341 scale-up Methods 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
本发明涉及嗜碱盐单胞菌(Halomonas alkaliphila)LH‑B.0040,属于生物技术领域。其保藏编号为CGMCC No.16324。该菌株可以在高盐无碳或低碳条件下彻底将硝酸盐转化为氮气,且不产生亚硝酸盐积累。本发明还公开了该菌株的菌剂,以及其处理高盐含氮废水的应用,该菌株最高可脱氮盐度为15%,在高盐含氮废水处理中很好的应用前景。同时能够在高盐环境下降解有机物,可用于高盐废水生物脱氮,能低成本解决高盐环境下生物脱氮难题。
Description
技术领域
本发明涉及环保生物技术领域,特别是一种嗜碱盐单胞菌(Halomonasalkaliphila)LH-B.0040,本发明还涉及前述菌株的微生物菌剂,及其在高盐含氮废水处理中的应用。
背景技术
高盐含氮废水是指总溶解固体(Total dissolved inorganic salt,TDS)质量分数高于1%的含氮废水称为,具有来源广泛、离子浓度高、污染物成分复杂等特点。如果未经处理直接排放,会导致江河湖泊的水质矿化度提高、水体富营养化、毒害水生生物并对土壤造成严重污染。全球高盐废水的处理需求已达到废水处理总需求的5%,而且还以年均2%的速率增长。
而硝酸盐引起的总氮污染问题是世界性的环保难题。各地立法组织纷纷出台相应法律规定,对水体中硝酸盐含量进行严格限定。我国也不断提高城镇污水和工业废水处理的总氮排放标准。大多C/N较低的污水处理厂仍主要靠投加大量的碳源实现TN的脱除,既不符合绿色环保发展理念,又导致一系列的负面作用,如产生大量污泥处置成本和二次污染风险,尾水碳超标、盐过高导致微生物死亡等。同时污水处理厂普遍存在尾水总氮不达标的问题。因此研究开发低碳甚至无碳的自养反硝化深度脱氮技术,是针对上述问题研究的热点。而微生物脱氮是一种低成本高效可行的方法。
生物处理技术是利用微生物的催化、同化等作用脱除废水中污染物的一种方法,具有应用广、适应性强、经济性好、处理效率高等特点。目前,低碳物探自养反硝化微生物处理技术的难点在于筛选耐盐反硝化深度脱氮菌种。利用某些细菌的硝化和反硝化的能力,将污水中的氮转化为无害的氮气,可以廉价无害的解决污水排放问题,尤其是自养反硝化细菌在不利用有机碳源的情况下,能够实现硝态氮的去除,是一种绿色环保的生物技术。但目前自养反硝化菌株的筛选及应用是技术难题,尤其是在高盐环境下实现自养脱氮的菌株更是鲜有报道。
发明内容
本发明所要解决的技术问题是针对现有技术的不足,提供一种新的嗜碱盐单胞菌LH-B.0040菌株,该菌株具有能够在高盐条件下进行自养反硝化脱总氮及碱度作用。
本发明所要解决的另一个问题是提供了含有嗜碱盐单胞菌LH-B.0040发酵液的微生物菌剂。
本发明的再一个问题是提供上述嗜碱盐单胞菌LH-B.0040的应用及含嗜碱盐单胞菌LH-B.0040菌剂的应用,主要是为解决高盐条件下的无碳生物脱氮问题,尤其是盐度≥10%条件下的无碳生物脱氮问题。
本发明的目的是通过以下技术方案实现:
本发明公开的嗜碱盐单胞菌(Halomonas alkaliphila)LH-B.0040,根据其形态特征和生理生化特征及16SrRNA基因序列在Genbank中的检索结果,分类命名为(Halomonasalkaliphila)LH-B.0040。该菌株保藏于中国普通微生物菌种保藏管理中心,保藏编号为CGMCC No.16324,保藏日期2018年8月20日。该菌株于2018年8月20日保藏于中国普通微生物菌种保藏管理中心,保藏编号为CGMCC No.16324。本发明的嗜碱盐单胞菌(Halomonasalkaliphila)LH-B.0040的16SrRNA序列长度为1400bp。
本发明的嗜碱盐单胞菌(Halomonas alkaliphila)LH-B.0040为革兰氏阴性菌;通过电子显微镜观察其形态结构呈棒、杆状,单个、无交叉,菌落表面光滑。
本发明的嗜碱盐单胞菌(Halomonas alkaliphila)LH-B.0040能够在盐浓度1%-15%、pH7-11、温度15-45℃范围内生长。最适生长温度是20℃,最适生长pH是9,最适生长盐度是1-10%。
本发明所述的嗜碱盐单胞菌(Halomonas alkaliphila)LH-B.0040于2017年3月25日从江苏省某海滩中采集样品中分离获得,其生长以硫铁矿、硫代硫酸钠等为电子供体,以NO3 -或NO2 -为电子受体生长。
本发明所述的微生物菌剂,包含以上技术方案所述的嗜碱盐单胞菌(Halomonasalkaliphila)LH-B.0040或者其发酵液的微生物菌剂。菌剂的制备方法可以采用现有技术中公开的常规菌剂的制备方法。
所述的微生物菌剂中嗜碱盐单胞菌(Halomonas alkaliphila)LH-B.0040含量不低于10%。微生物菌剂可以按需要添加适量的菌剂助剂,相互间不排斥的同功能菌株发酵液等。也可以全部是嗜碱盐单胞菌(Halomonas alkaliphila)LH-B.0040菌株或者菌种发酵液。
本发明所述的微生物菌剂在高盐废水处理中的应用。
本发明所述的微生物菌剂在盐度1-15%的高盐废水的生物脱氮处理中的应用。
本发明所述的微生物菌剂进一步优选对于盐度大于10%的高盐废水进行自养反硝化。
利用本发明菌株LH-B.0040制备的微生物菌剂,当以生物脱氮为主要目标进行应用时,该菌剂的最适耐盐浓度范围是1%-15%。
当废水盐浓度为1%-15%,该菌剂可以NO3-N、NO2-N为电子受体,以硫铁矿、硫代硫酸钠等为电子供体,在无碳的情况下,经过6h,NO3-N、NO2-N的去除率可以达到99.5%以上,无亚硝酸盐积累,且对碱度有一定的去除效果。
本发明与现有技术相比,有益效果如下:
本发明的嗜碱盐单胞菌LH-B.0040耐受高渗透压及高盐环境,其耐盐范围是1%-15%,可在该盐度范围内以NO3-N、NO2-N为电子受体、以硫铁矿、硫代硫酸钠等为电子供体进行反硝化脱氮,硝化反硝化效率均大于99.5%,不产生亚硝酸盐积累,且对碱度有一定的去除效果。
利用LH-B.0040制备的微生物菌剂,耐受高渗透压及高盐环境,在高盐废水处理、RO浓水处理、尾水净化、高碱度废水脱氮、污染海水治理,盐碱地修复,消耗氮素营养,抑制藻类过度繁殖,净化水体,改良底质等方面有较好的应用效果。
附图说明
图1为菌株系统进化树分析图;
图2为菌株革兰氏染色后光学显微镜形态图;
图3为菌株电子显微镜形态图;
图4菌株在不同盐度下的生长曲线图。
具体实施方式
以下结合具体实施例对本发明作进一步说明,但本发明并不限于以下实施例。下列实施例中,如无特殊说明,均为常规方法。
实施例1,嗜碱盐单胞菌LH-B.0040的筛选
1、菌群富集:将采集的样品经1%盐水反复清洗5次,去除残留COD,经测定上清液COD<10mg/L。
配置自养反硝化培养液:每升含KNO3 0.4g/L,硫铁矿粉末1.5g/L、硫代硫酸钠0.2g/L、多硫化钙0.2g/L、菱铁矿石粉末1g/L、铁粉末0.5g/L、硫酸亚铁0.3g/L、Na2CO3 0.5/L、NaCl 1%、微量元素液10mL,pH8-9。微量元素液(1000mL):MnCl2·7H2O 0.05g/L,H3BO30.05g/L,CoCl2·6H2O 0.2g/L,CuCl2·6H2O 0.05g/L,NiCl2·6H2O 0.05g/L,Na2Mo4·2H2O0.05g/L,水1L。
采用缺氧反应器中加入一半体积的反硝化培养液,将清洗好备用的耐盐反硝化浓缩污泥接入反应器中,补清水至系统MLSS3000-5000mg/L浓度,开启设备搅拌,控制溶氧<0.5mg/L,15-37℃培养8-48h,测定硝态氮和亚硝态氮含量均<1mg/L时,说明反硝化完全,停止搅拌,沉降2h,弃去上清液一半,继续补加反硝化培养液一半后,开启搅拌反应,如此不额外补加任何碳源的条件下重复10次以上,反应时间逐渐缩短至8h内,出水硝态氮和亚硝态氮含量均1mg/L,表明反硝化菌群富集成功。
2、菌种分离鉴定
将实富集的自养反硝化菌液,进行梯度稀释后划线或涂布分离;
自养反硝化固体分离培养基:每升含KNO3 0.4g/L,硫铁矿粉末2g/L、硫代硫酸钠0.3g/L、多硫化钙0.2g/L,菱铁矿石粉末0.5g/L、铁粉末0.5g/L、硫酸亚铁0.5g/L,Na2CO30.5g/L,NaCl 10g/L、微量元素液10mL,pH8-9,琼脂粉20g。微量元素液(1000mL):MnCl2·7H2O 0.05g/L,H3BO3 0.05g/L,CoCl2·6H2O 0.2g/L,CuCl2·6H2O 0.05g/L,NiCl2·6H2O0.05g/L,Na2Mo4·2H2O 0.05g/L,煮沸充分溶解琼脂,倒制平板培养基备用。
将梯度稀释后的自养反硝化菌液在生物安全柜中涂布至固体平板上,放置于培养箱37℃下培养2-5d,待长出菌落后,挑取单菌落划线分离纯化,直至平板中的菌呈单一菌种。
将分离获得的单菌株送至中国农业微生物菌种保藏中心进行菌种鉴定,单菌株鉴定为嗜碱盐单胞菌(Halomonas alkaliphila),命名为嗜碱盐单胞菌LH-B.0040。菌株系统进化树分析图参见图1。
实施例2,嗜碱盐单胞菌LH-B.0040的形态特征及生理生化
将筛选出的菌进行革兰氏染色后(如图2),光学显微镜观察为革兰氏阴性菌,呈短杆状。经电子显微镜观察(如图3)呈棒、杆状,单个、无交叉,菌落表面光滑。
在无菌条件下,用移液枪吸取50μL菌液,接种到15种生理生化试剂反应管(葡萄糖、蔗糖、甘露糖、麦芽糖、乳糖、氧化酶、淀粉、苯丙氨酸脱氨酶、甘露醇、七叶苷、硝酸盐还原(还原)、亚硝酸盐(产气)、明胶、DNA、尿素),将其在试剂架上摆放整齐,放至30℃培养箱中培养24h观察并记录结果,如表1所示:
表1嗜碱盐单胞菌LH-B0040的生理生化特征
注:+:阳性;-:阴性。
实施例3,嗜碱盐单胞菌LH-B.0040的耐盐生长特性
将LH-B.0040接入不同盐度的自养反硝化培养液中,放入35℃,160rpm摇床中培养,每4h测定一次OD600,待连续两次以上测量值没有明显变化后停止测量。结果表明(见图4),菌株LH-B.0040接接种于不同盐度的液体培养基中,在3-15%盐度培养基中,接种后直接进入对数期,没有延迟期,25h进入稳定期;说明该菌株耐盐性能良好。
实施例4,嗜碱盐单胞菌LH-B.0040的脱氮性能测定
将筛选的菌株接入盐浓度为5%的自养反硝化培养液中培养24h后,一级种子液以10%接种量接种于盐浓度为5%自养反硝化培养液,培养12h后以20%接种量接种于自养反硝化培养基中(初始氮浓度为55mg/L),每2h取样测定、硝态氮和亚硝态氮浓度。结果表明,菌株LH-B.0040可以有效转化硝态氮,可在2h内将其全部转化,在反应液中几乎检测不到亚硝态氮。说明该菌株有良好的反硝化脱氮能力,且反硝化速率快。
实施例5,嗜碱盐单胞菌LH-B.0040的发酵液制备
(1)培养基:KNO3 0.4g/L,硫铁矿粉末1g/L、硫代硫酸钠0.3/L、硫酸亚铁0.5/L,Na2CO3 1.5g/L,NaCl 10g/L;微量元素10ml/L,pH为8.5~9.0。液体培养基进行灭菌,同时控制好水分蒸发。
(2)上述培养基装入1L的三角瓶中,接种菌株LH-B.0040后在35℃条件下培养,培养过程中补充水分的散失,防止盐浓度的变化。培养48h后测定反硝化降解率,硝态氮降解率达到90%以上。培养物进行两次转接培养,每次转接后均培养48h,两次培养完成后硝态氮降解率达到90%以上。
(3)将1L三角瓶中的培养液接种到20L的厌氧发酵罐中,反复进行发酵培养,培养基不变。培养时间48h,培养温度为35℃、搅拌速度为100rpm,菌液硝态氮降解率达到90%以上即为发酵成功。
(4)检验:培养过程中使用显微镜每日取样观察,检查是否有杂菌混入生长;同时观察LH-B.0040的生长及形态变化情况。
(5)结果:试验结果表明,菌株LH-B.0040可以快速的进行发酵培养和放大,这表明LH-B.0040具有规模应用的潜力。
实施例6,含菌株LH-B.0040的微生物菌剂的制备
利用实施例5所述方法制备的菌株LH-B.0010的发酵液作LH-B.0040的微生物菌剂。
实施例7,含菌株LH-B.0040的微生物菌剂在高盐含氮废水处理中的应用
利用实施例6制得的微生物菌剂,处理COD100mg/L,NO3-N 100mg/L,pH7.0-8.5,盐度分别为1%、3%、5%、8%、10%、15%的废水,过程中不补加碳源,30℃、2h后各物质如下表2所示(单位%):
表2
盐度 | 1% | 3% | 5% | 8% | 10% | 12% | 15% |
硝态氮 | 0.09 | 0.23 | 0.34 | 0.35 | 0.48 | 0.72 | 1.10 |
亚硝态氮 | 0.012 | 0.01 | 0.02 | 0.01 | 0.01 | 0 | 0.02 |
由上表2可知,复合微生物菌剂能够在1%-15%盐度下高效降解有机物及硝态氮,且废水中几乎没有亚硝酸盐氮的积累,几乎完全实现了完全反硝化,15%盐度条件下,硝酸盐氮有少量的积累,延长培养时间后硝酸盐氮完全脱除,说明在高盐度的条件下,需要延长处理时间。
实施例8,含LH-B.0040菌株的微生物菌剂在实际高盐含氮废水处理中的应用实验:将实施例6制得的微生物菌剂用于处理COD100mg/L,总氮150mg/L(主要为硝态氮),盐度3%的焦化废水RO浓水,过程中不补加碳源,稳定运行后,总氮去除率95%以上。证明该菌剂对于高含盐废水具有很好的处理效果。
Claims (9)
1.一种嗜碱盐单胞菌(Halomonas alkaliphila)LH-B.0040,其特征在于,该菌株的保藏编号为CGMCC No.16324。
2.根据权利要求1所述的嗜碱盐单胞菌LH-B.0040的用途,其特征在于,该菌株能够在低有机碳源或无有机碳环境下降硝酸盐还原为氮气,实现自养或自养/异养协同脱氮。
3.根据权利要求2所述的用途,其特征在于,嗜碱盐单胞菌LH-B.0040在碱度>400mg/L的废水中脱氮并降低碱度。
4.根据权利要求1-3中任何一项所述的用途,其特征在于,嗜碱盐单胞菌LH-B.0040在盐度1%-15%的环境下进行硝态氮及碱度的脱除。
5.一种微生物菌剂,其特征在于:该微生物菌剂含有权利要求1所述的菌株嗜碱盐单胞菌LH-B.0040的菌种发酵液。
6.根据权利要求5所述的微生物菌剂,其特征在于,所述的微生物菌剂中嗜碱盐单胞菌LH-B.0040的菌种发酵液的质量含量不低于10%。
7.权利要求5或6所述的微生物菌剂的用途,其特征在于,所述的用途为将该菌剂应用于盐度1%-15%的高盐废水反硝化脱总氮的处理。
8.根据权利要求7所述的用途,其特征在于,所述的反硝化为缺氧反硝化。
9.根据权利要求7所述的用途,其特征在于,所述的用途为将该菌剂应用于盐度10%-15%的高盐废水反硝化脱总氮的处理。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202310620931.0A CN116590190A (zh) | 2023-05-29 | 2023-05-29 | 嗜碱盐单胞菌lh-b.0040及其微生物菌剂与用途 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202310620931.0A CN116590190A (zh) | 2023-05-29 | 2023-05-29 | 嗜碱盐单胞菌lh-b.0040及其微生物菌剂与用途 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN116590190A true CN116590190A (zh) | 2023-08-15 |
Family
ID=87608067
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202310620931.0A Pending CN116590190A (zh) | 2023-05-29 | 2023-05-29 | 嗜碱盐单胞菌lh-b.0040及其微生物菌剂与用途 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN116590190A (zh) |
-
2023
- 2023-05-29 CN CN202310620931.0A patent/CN116590190A/zh active Pending
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN109456926B (zh) | 一种含嗜盐反硝化菌yl5-2的微生物菌剂及其应用 | |
CN111117914B (zh) | 一种耐盐异养好氧硝化细菌菌株、培养方法、菌液及应用 | |
CN113604379B (zh) | 一株具有异养硝化-好氧反硝化功能的全海假单胞菌及其应用 | |
CN110791444B (zh) | 一种施氏假单胞菌、采用该施氏假单胞菌制得的复合菌剂及该复合菌剂的应用 | |
CN114703095B (zh) | 一株成都假单胞菌及其在污废水净化领域的应用 | |
CN109456913B (zh) | 铜绿假单胞菌及应用其处理有机废水的方法 | |
CN115125164B (zh) | 一株高度耐盐、耐重金属异养硝化-好氧反硝化自絮凝海洋菌及其在群落构建中的应用 | |
CN110699285B (zh) | 一种巴利阿里假单胞菌及其在处理垃圾渗滤液膜浓缩液中的应用 | |
CN109337832A (zh) | 一种耐高氨氮异养硝化-好氧反硝化的苍白杆菌及其应用 | |
CN114908016A (zh) | 一株脱氮假单胞菌及其在污废水净化领域的应用 | |
CN114395505A (zh) | 一种低温反硝化菌及其应用 | |
Yang et al. | Using ammonium‐tolerant yeast isolates: Candida halophila and Rhodotorula glutinis to treat high strength fermentative wastewater | |
CN112266885A (zh) | 一种异养硝化好氧反硝化菌y16及其应用 | |
CN115386520B (zh) | 一株嗜吡啶红球菌rl-gz01菌株及其应用 | |
CN109055259B (zh) | 假单胞菌xd-3及其应用以及微生物絮凝剂 | |
CN109052624B (zh) | 一种移动式净化污水和黑臭水体的方法及装置 | |
CN114292798B (zh) | 一种厌氧反硝化菌种及其在河道水体修复中的应用 | |
CN113583897B (zh) | 一株阿氏芽孢杆菌fl05及其应用 | |
CN105039225A (zh) | 一种好氧反硝化菌及其应用 | |
CN113215027B (zh) | 水生产碱杆菌as1及其在污水处理中的应用 | |
CN115057523A (zh) | 一种sbr法废水处理装置及其处理方法 | |
CN116590190A (zh) | 嗜碱盐单胞菌lh-b.0040及其微生物菌剂与用途 | |
Sang et al. | Simultaneous removal of carbon, nitrogen and phosphorus from hypersaline wastewater by bioaugmented intermittently aerated biological filter (IABF) | |
CN115305218A (zh) | 高原芽孢杆菌sx-3及其在降解工业污水中的应用 | |
CN110468066B (zh) | 一种好氧反硝化菌株及其应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination |