CN1165800A - Improver of rhizosphere microflora - Google Patents
Improver of rhizosphere microflora Download PDFInfo
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- CN1165800A CN1165800A CN 97104966 CN97104966A CN1165800A CN 1165800 A CN1165800 A CN 1165800A CN 97104966 CN97104966 CN 97104966 CN 97104966 A CN97104966 A CN 97104966A CN 1165800 A CN1165800 A CN 1165800A
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
- C09K17/18—Prepolymers; Macromolecular compounds
- C09K17/24—Condensation polymers of aldehydes or ketones
- C09K17/28—Urea-aldehyde condensation polymers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/34—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the groups, e.g. biuret; Thio analogues thereof; Urea-aldehyde condensation products
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
- C05C9/02—Fertilisers containing urea or urea compounds containing urea-formaldehyde condensates
Abstract
An improver of rhizosphere microflora contains a urea-formaldehyde condensate of formula X-(NH-CO-NH-CH2)n-Y (I), where X = H or hydroxymethyl; Y = ureido or hydroxy, and n = 4 or more. According to the invention, the improver of rhizosphere microflora is capable of predominantly proliferating the microfloras in a rhizosphere for a long period.
Description
The present invention relates to a kind of modifying agent of rhizosphere microflora, it contains the melocol condenses as active ingredient.
Various soil improvement agents have conventionally been developed as material that contains compost, chitosan or its analogue and the material that contains microorganism, improve the soil to improve its effect (Japan's special permission 39476/1990 to plant-growth, with Japanese Patent Application Publication special permission 82112/1995,127824/1978,253901/1992,181637/1994,107512/1994 and 165523/1995).These soil improvement agents join in the soil to stop Plant diseases and to promote plant-growth.
Though using the soil of these soil improvement agents is useful when suppressing the breeding of plant pathogeny microorganism, it is difficult breeding the useful microorganism of a group highlightedly.
The modifying agent that the purpose of this invention is to provide a kind of rhizosphere microflora.
As positive result of study, the present inventor finds the special water-insoluble melocol condenses useful microorganism of breeding a group in rhizosphere microflora highlightedly, finishes the present invention with this.
Promptly the invention provides a kind of modifying agent of rhizosphere microflora, it contains the melocol condenses by the general formula I representative as active ingredient:
X-(NH
2-CO-NH-CH
2)
n-Y (I) wherein X be hydrogen atom or-CH
2OH, Y are-NHCONH
2Or-OH and n are 4 or bigger integer.
Describe the present invention hereinafter in detail.
Modifying agent according to rhizosphere microflora of the present invention contains water-insoluble and biodegradable melocol condenses as active ingredient.
The water-soluble of it restrains water or littler less than 0.01g/100.Its microbic resolvability refers to that it is changed ammonification by microorganism and/or with the ability of the nitrogen of nitric acid form.
By under alkaline condition, urea and formaldehyde reaction being provided its hydroxymethyl derivative, under acidic conditions, it is dewatered and condensation then, then from the condenses that obtains, remove the soluble component of hot water (being the part of n among the formula I＜4) and just can obtain melocol condenses of the present invention.
The suitable formaldehyde and the mol ratio of urea are 0.8 to 1.3.It is invalid being less than 0.8 mol ratio, because can generate a large amount of hot water solubilized components, is not easy to biodegradable product and produce less hot water solubilized component but provide greater than 1.3 mol ratio.Can add urea when its hydroxymethyl derivative of preparation, reaching said mol ratio at last, this is reflected at 20 to 95 ℃ and carried out 0.5 to 30 hour, reacts under weakly alkaline pH in batches, and preferred pH7 to 8 is with regulating as alkaline matters such as sodium hydroxide.
Reaction product is by forming as the dimethylolurea of principal constituent with as methylolurea, trimethylol urea and the free formaldehyde of submember.
Dehydrogenation/condensation reaction at PH3 to 5, was carried out 0.5 to 5 hour under 60 to 80 ℃ of the temperature in the presence of as the acidic substance of stiffening agent, as the acidic substance of stiffening agent sodium pyrosulfate, sal enixum was for example arranged, sodium hydrogen phosphate, potassium hydrogen phosphate, phosphoric acid, sulfuric acid, acetate, citric acid and tartrate.
Be immersed in 60 ℃ or more can remove hot water solubilized component in the pyritous hot water by the condenses that will obtain, preferred 80 ℃ or pyritous hot water more carried out 0.5 to 3 hour, then stirred and centrifugal to make it dehydration.End product is the water insolubility and the biodegradable condenses of the white powder form of particle diameter scope between 0.5 to 5mm, and about 40 weight percents of its water-content are represented by general formula I
X-(NH
2-CO-NH-CH
2)
n-Y????(I)
Wherein X be hydrogen atom or-CH
2OH, Y are-NHCONH
2Or-OH and n are 4 or bigger integer.
According to of the present invention, suc as formula the n shown in the I be 4 or the modifying agent of the rhizosphere microflora of bigger integer be biodegradable.In the present invention, hot water solubilized component is removed from condenses.This is because described n is 4 or is degraded by microorganisms soon and is dissolved in the water and in water less than 4 solubilized component and run off, can be for a long time the useful microorganism of breeding a group in root district microscopic flora highlightedly.
Because the melocol condenses of Sheng Chaning will be by the useful microbiological deterioration in the rhizosphere microflora in the above described manner, the useful microorganism of a group that makes in rhizosphere microflora of using of described modifying agent breeds significantly.The breeding of the microorganism that a group is useful causes stoping Plant diseases, and modifying agent itself degraded gradually by microorganism can be by the nitrogen of the ammonia of plant utilization and/or nitric acid form to discharge.
Used vegetation type is not particularly limited, and the improvement that specifically is included in turf for example, pasture, vegetables, root vegetables, flowering plant, fruit tree, tea tree and transplants the root district microscopic flora in the tree.
Useful microorganism comprises the microorganism that belongs to Rhodopseudomonas, Agrobacterium, Bacillus in rhizosphere microflora.
The amount of described modifying agent is every liter of soil 0.5g to 10g normally, preferred 2-6g, even but use with the amount that surpasses above-mentioned scope, modifying agent of the present invention can not cause any problem to plant yet.This may be due to the fact that: modifying agent of the present invention be water insoluble and when plant-growth by root bacterial flora degraded and change into gradually can be by the nitrogen of plant utilization.Modifying agent is preferably used in a large number, because the number of times of using can reduce.Modifying agent can be applied directly in the soil by the hardened product or to use with the miscellany of fertilizer or other solid modifying agent etc.
Hereinafter, the present invention describes in more detail by reference example, but it does not refer to limit scope of the present invention.
Preparation embodiment 1, the preparation of melocol condenses
(urea: formaldehyde=1: 2.3 mol ratio) be adjusted to pH7.8 with aqueous sodium hydroxide solution, mixture under agitation reacted 1 hour down at 90 to 95 ℃ urea-37% formalin mixture.Then, reaction mixture was adjusted to pH4 with sal enixum, 90 to 95 ℃ of reactions 3 hours.
Urea is added in the initial melocol condenses aqueous solution that obtains again, becomes 1: 1.2 up to the ratio of all ureas and formaldehyde, mixture was 80 ℃ of reactions 1 hour then.
The reactor product that obtains is transferred in the kneading machine and stirs and reaches 70 to 80 ℃ up to its temperature, and 80% phosphoric acid with 1.5 volume percent adds wherein then.
Mixture remains on 80 ℃ and reacted 3 hours in kneading machine, under agitation its hardened product of dewatering gradually and providing hydration.
The hardened product of this hydration is immersed in 80 ℃ of hot water and stirred 1 hour, the centrifugal then end product (n 〉=4) that provides.
Embodiment 1 rhizosphere microflora modifying agent (1)
The melocol condenses of producing by above-mentioned preparation embodiment 1 uses (hereinafter being called " modifying agent of the present invention ") as the modifying agent of rhizosphere microflora.
Measure the assimilability of each bacterial strain.
(1) the microorganism assimilability that belongs to Rhodopseudomonas is tested
Pseudomonas aeruginosa IFO12689, Pseudomonas fluorescens IFO14160 and pseudomonas putida IFO14164 use as experimental strain.These bacterium dwarfs in 30 ℃ of overnight incubation, are immersed in the stroke-physiological saline solution standby as experimental bacteria liquid in the plain agar slant medium then.
Used basic nutrient solution is to be used for not testing substratum to the assimilability of the Rhodopseudomonas that wherein adds ammonium sulfate (nitrogenous source).(R.Y.Stanier，et?al.J.Gen.Microbiol.，43，159(1966))。After the sterilization of basic nutrient solution, disinfectant modifying agent of the present invention in advance, glucose or ammonium sulfate add wherein with the ratio shown in the table 1, and aseptic condition inhale down move to the aseptic experiment test tube with preparation experiment nutrient solution (1) to (4).
Group component that table 1 adds (g)/1000ml basic culture solution test component that nutrient solution adds (1) (2) (3) (4) modifying agent 2 of the present invention---glucose 11 1-ammonium sulfate-1-1
-: do not add.
Each above-mentioned test organisms liquid is seeded in the above-mentioned test nutrient solution with the platinum circle and 30 ℃ of cultivations.After 7 days cultivate, measure the turbidity in each nutrient solution.Can judge: with turbidity (+) but be illustrated in modifying agent assimilate into carbon source of the present invention or nitrogenous source in the nutrient solution, and can not with turbidity (-) expression.
Be displayed in Table 2 the result.
The assimilability experiment of the fluorescence microorganism of table 2 Rhodopseudomonas
Experimental strain
Experiment substratum green pus bar Pseudomonas fluorescens pseudomonas putida
Bacterium IFO14160 IFO14164
IFO126
89 (1) modifying agent+Portugals of the present invention+++grape sugar (2) glucose+ammonium sulfate+++(3) glucose---(4) ammonium sulfate---
(2) belong to the experiment of the microorganism assimilability of Agrobacterium
Agrobacterium radiobacter IFO13532 uses as experimental strain.Bacterial strain in 30 ℃ of overnight incubation, is immersed in the stroke-physiological saline solution standby as experimental bacteria liquid in the plain agar slant medium then.
Used basic substratum prepares according to the same way as that the preparation Rhodopseudomonas as above-mentioned (1) can assimilate the substratum of test.Modifying agent of the present invention, glucose or ammonium sulfate are sterilized in advance, add wherein with the ratio shown in the table 3, and aseptic condition inhale down move to the aseptic experiment test tube with preparation experiment nutrient solution (1) to (3).
Group component that table 3 adds (g)/1000ml basic culture solution
Test medium
Institute adds component (1) (2) (3)
Modifying agent 2 of the present invention--
Ammonium sulfate-1-
Glucose 111
-: do not add.
Above-mentioned test organisms liquid is seeded in above-mentioned each test nutrient solution and with the same way as of above-mentioned (1) with the platinum circle and cultivates.Measure it and whether have assimilability.
Be displayed in Table 4 the result.
The experiment of table 4 agrobacterium radiobacter assimilability
Experiment substratum experimental result 1 modifying agent+glucose of the present invention+(slowly) 2 glucose+ammonium sulfate+3 glucose-
(3) belong to the experiment of the microorganism assimilability of Bacillus
Bacillus subtilus JCM1465 uses as experimental strain.This bacterial strain in 30 ℃ of overnight incubation, is immersed in the stroke-physiological saline solution standby as experimental bacteria liquid in the plain agar slant medium then.
Used basic nutrient solution prepares according to the same way as of testing substratum as the preparation pseudomonas assimilability of above-mentioned (1).Modifying agent of the present invention, glucose or ammonium sulfate are sterilized in advance, add wherein with the ratio shown in the table 5, and aseptic condition inhale down move to the aseptic experiment test tube with preparation experiment nutrient solution (1) to (3).
Group component that table 5 adds (g)/1000ml basic culture solution
Test medium
Institute adds component (1) (2) (3)
Modifying agent 2 of the present invention--
Ammonium sulfate-1-
Glucose 111
-: do not add.
Above-mentioned test organisms liquid is seeded in above-mentioned each test nutrient solution and with the same way as above-mentioned (1) with the platinum circle and cultivates.Whether measure its assimilability exists.
Be displayed in Table 6 the result.
The assimilability experiment of table 6 Bacillus subtilus
Experiment substratum experimental result 1 modifying agent+glucose of the present invention+2 glucose+ammonium sulfate+3 glucose-
(4) experiment of ray fungi assimilability
Streptomyces albus subgenus white (Strepto myces albus subsp.albus) IFO14626, Nocardiopsis dassbnvillei subsp.dassonv illei IFO 14626, bronze small single-cell bacteria JCM3031, star nocardia JCM3384 and strep toverticillium baldacciiJCM4272 are as experimental strain.Each cultivation bacterium is centrifugal then to cultivate for 1 week under 30 ℃ of stirrings of each bacterial strain in yolk one yeast juice substratum.Wash cultured microorganism like this several times with stroke-physiological saline solution, be immersed in the stroke-physiological saline solution standby then as experimental bacteria liquid.
After the minimum medium sterilization of Williams etc., modifying agent of the present invention, altheine or ammonium sulfate are sterilized in advance, add wherein with the ratio shown in the table 7, pour in the sterile chamber then and solidify with preparation experiment with substratum (1) to (4).
Group component that table 7 adds (g)/1000ml minimum medium
Component that test medium adds (1) (2) (3) (4) modifying agent 1 of the present invention---altheine-1--ammonium sulfate--1-
-: do not add.
Substratum (1000ml) proportioning is as follows
Substratum such as Williams
Glucose 10g
MgSO
4·7H
2O??0.5g
NaCl???????????0.5g
FeSO
4·7H
2O??0.01g
K
2HPO
4???????1g
Agar 15g
Distilled water 1000ml
Carried out disinfection in 15 minutes by modifying agent of the present invention being put into boiling water, altheine and ammonium sulfate use filter-sterilized.
Each above-mentioned test organisms liquid is seeded in 1 to 4 test medium with the platinum circle and 30 ℃ of cultivations.After 15 days cultivate, measure the bacterial growth degree in each substratum, and assess the bacterial growth degree with experiment substratum 2 and 4 as positive and negative contrast respectively.This assessment is carried out according to the as above same way as of (1).
Be displayed in Table 8 the result.
The experiment of table 8 ray fungi assimilability
Nitrogenous source (test medium number) test strain modifying agent altheine of the present invention ammonium sulfate does not add
(1) (2) (3) (4) streptomyces albus subgenus white IF013014-++-N.dassonvilltisubsp.dasson villei IFO14626-++-bronze small single-cell bacteria JCM3031-+--star shaped card slave Salmonella JCM3384-++-S.baldaccii JCM4272-+--
(5) experiment of fungi assimilability
Pathogeny fungi in the soil, rose-colored sickle spore (Fusarium roseum) IFO7189, dry thread Pyrenomycetes IFO30936 and dry thread Pyrenomycetes IFO30981 use as experimental strain.Each bacterial strain in 25 ℃ of 2 weeks of cultivation, is immersed in the stroke-physiological saline solution that contains Spheron MD 30/70-80 standby as experimental bacteria liquid in potato dextrose agar plate culture medium then.
Used experiment substratum is yeast carbon back nutrient agar (Difco) (11.7g yeast carbon back nutrient agar (Difco) and the 15g agar in 1000ml distilled water).
The experiment substratum is dissolved, and sterilization is in about 50 ℃ of coolings down.Each experimental bacteria liquid is seeded to substratum with 1/20 volume with respect to substratum, and is mixed, and pour in 3 aseptic containers and solidify to prepare 3 agar plates.A small amount of modifying agent of the present invention is joined in one of them agar plate, and small amount of ammonium sulfate adds in another, and last does not add.Each flat board is in 25 ℃ of cultivations.After cultivating in 14 days, the determination experiment microbial growth.The modifying agent of the present invention that uses was above sterilized in boiling water 15 minutes and dry air.
Table 9 display result.
The assimilability experiment of table 9 fungi
Nitrogenous source experimental strain modifying agent ammonium sulfate of the present invention do not add rose-colored sickle spore IFO7189-+-dry thread Pyrenomycetes IFO30936-+-dry thread Pyrenomycetes IFO30981-+-(6) found that from above, similar with ammonium sulfate, modifying agent of the present invention is belonged to useful Institute of Micro-biology's assimilation of Pseudomonas fluorescens, Agrobacterium and Bacillus.Further find that ammonium sulfate is that actinomycetes and pathogeny fungi in the soil assimilates by harmful microorganism, and modifying agent of the present invention is by such Institute of Micro-biology's assimilation.Therefore, modifying agent of the present invention is good, because it is suitable for the selectivity breeding of useful microorganism.Embodiment 2 rhizosphere microflora modifying agents (2)
Measure modifying agent of the present invention to the effect of micropopulation in a meadow (pen cross bent).
Microorganism (seeing below) in sample (1) and (2) is diluted on flat board, cultivates 7 or 14 days in 25 ℃ in the agar nutrient solution that contains antifongin or microbiotic (seeing table 10) as shown in table 10.After the cultivation, measure the microbe population of living in every gram sample.
The result is as shown in table 10.
The microbe population of living in the every gram sample of table 10
Ratio ※: the ratio of microbe population in microbe population and the sample (2) in the sample (1).
| Kind | Nutrient agar | Culture condition | Sample (1) | Sample (2) | Ratio * |
| Bacillus | The SCD that contains anti-mycotic agent | 25 ℃, 7 days, aerobic | 1.1×10 5 | ?1.8×10 3 | ????61 |
| Pseudomonas fluorescens | The King B that contains anti-mycotic agent | 25 ℃, 7 days, aerobic | 7.0×10 4 | ?7.0×10 3 | ????10 |
| Ray fungi | The albumin that contains anti-mycotic agent | 25 ℃, 14 days, aerobic | 2.8×10 6 | ?7.0×10 5 | ????4 |
| Filamentous fungus | The PD that contains paraxin | 25 ℃, 7 days, aerobic | 7.0×10 3 | ?3.0×10 3 | ????2 |
In the table 10, sample (1) is the root region soil of collecting from meadow in June nineteen ninety-five, modifying agent of the present invention in March, 1993 with 200g/m
2(the 10cm degree of depth) joins on that piece meadow, and sample (2) is never to add the root region soil (contrast) of collecting in the meadow of modifying agent of the present invention and coming June nineteen ninety-five.
As what can see from table 10, belong to microorganism that Bacillus and Pseudomonas fluorescens belong to and bred 61-and 10 times in 2 years respectively, and ray fungi, fungies etc. have only bred 2-and 4-doubly compared with the control, show that the micropopulation useful in the root district breeds with being highlighted.
Embodiment 3 rhizosphere microflora modifying agents (3)
To measure the assimilability of each bacterial classification as the same way as of embodiment 1.
(1) normal soil microorganism assimilability experiment
Bacillus megatherium IFO15308, livid purple look bacillus IFO12614, enteroaerogen IFO13534, xanthomonas campestris IFO13551, the joyous Wen bacterium of carrot soft subspecies caroto vora IFO14082 bacterium Bacteria erwinia uses as experimental strain, and measures them with the same way as of false pseudomonas bacillus assimilability experiment as described above.
Join in the substratum carbon source and nitrogenous source as shown in Table II.
The added component quantity of Table II (g)/1000ml basic culture solution
Test medium
Institute adds component (1) (2) (3)
Modifying agent 2 of the present invention--
Ammonium sulfate-1-
Glucose 222
-: do not add.
The result is as shown in table 12.
The assimilability experiment of the soil microorganisms that table 12 is common
Nitrogenous source (experiment substratum number) experimental strain modifying agent ammonium sulfate of the present invention does not add
(1) (2) (3) Bacillus megatherium IFO15308-+-livid purple look bacillus IFO12614++-enteroaerogen IFO13534++-the joyous Wen bacterium of carrot soft subspecies++-the CarotovoraIFO14082 xanthomonas campestris-+-IFO13551
(2) soil pathogeny ray fungi assimilability experiment
Known is that the shot hole streptomycete IFO13767 of potato scab uses as experimental strain, and with as embodiment 1, the same way as of (4) described ray fungi assimilability experiment is measured them.
The nitrogenous source that joins in the substratum is identical with table 7.
The result is as shown in table 13.
Table 13 shot hole streptomycete IFO13767 is to the assimilability experiment as the modifying agent of the present invention of nitrogenous source
Experiment substratum (nitrogenous source) experimental result
Modifying agent of the present invention-
Altheine+
Ammonium sulfate+
Do not add-
(3) soil pathogeny fungi assimilability experiment
Garden Dahlia wheel branch spore IFO9939, sharp sickle spore IFO31631, mulberry volume load bacterium IFO31651, ultimate rotten bacterium IFO32612, IFO30696 uses as experimental strain with Phytophthora capsici, and with as embodiment 1, the same way as of (5) described fungi assimilability experiment is measured them.
The result is as shown in table 14.
The experiment of table 14 soil pathogeny fungi assimilability
Nitrogenous source experimental strain modifying agent ammonium sulfate of the present invention do not add a Garden Dahlia wheel branch spore IFO9939-+-sharp sickle spore IFO31631-+-Sang volume load bacterium IFO31651-+-ultimate rotten bacterium IFO32612-+-Phytophthora capsici IFO30696-+-
Above result shows that modifying agent of the present invention can be by most of normal soil microbial assimilation, but by potato scab or the assimilation of common pathogeny fungi.Can find out that from these results and the result among embodiment 1 modifying agent of the present invention is not assimilated by ray fungi or pathogeny fungi, it is an excellent material as nitrogenous source, relies on useful microorganism optionally to grow.
Embodiment 4 rhizosphere microflora modifying agents (4)
Mensuration is from watermelon (kind: the effect of the modifying agent of the present invention of rhizosphere microflora uji crossbred Shiratori watermelon).Used soil is that seedling early growth is cultivated soil, Gen kikun No.1 (Coop Chemical), this modifying agent adds wherein with 0% to 4%, watermelon seed is planted in the basin that contains 1L soil, and under artificial condition, cultivated 75 days, in 25 ℃ of placements 12 hours, placed 12 hours in 18 ℃ in the dark their every days under 5000 illumination of reining in.According to ShuichiIshizawa and Tatsuhiko Suzuki (＂ Dojyobiseibutsu No Seitai ＂ (soil microorganisms ecology), ＂ Seitaigaku Kouza (ecological course) 24 ＂, PP.113-115, (1973), publish by Kyoritsu Shuppan K.K.) described fractionating method in water prepares non-root region soil, root region soil, and rhizoplane.In the experimental technique, nutrient agar composition etc. and embodiment 2 is identical.
The result is presented in the table 15.The fresh weight of the root of each plant is 13.2g in contrast in (0% modifying agent of the present invention), is 18.8g in 4% modifying agent of the present invention, promptly the growth fraction of latter's root the former up to about 1.4 times.
Table 15-1
Growth of watermelon 75 days afterwards
The microbe population (every 1g dry ground earth) that root region soil is lived
B/F
The present invention improves this improvement of dosage living organism dosage
The 0% 4% 0% 4% aerobic little lifes of body ratio microorganism nutrient agar contain 1.8 * 10 of anti-mycotic agent
91.8 * 10
91.0 the aerobic little life of 16.4 11.3 thing SCD contains 3.3 * 10 of anti-mycotic agent
92.2 * 10
90.7 30.0 13.8 thing albumin bacilluss contain 1.1 * 10 of anti-mycotic agent
72.8 * 10
7Contain 1.8 * 10 of anti-mycotic agent 2.5 0.1 0.2 belong to the SCD gram-negative
82.2 * 10
81.2 1.6 1.4 property microorganism CVT gram-negatives contain 5.3 * 10 of anti-mycotic agent
83.5 * 10
80.7 4.8 2.2 property microorganism Columbia CNA fluorescence vacations singly contain 7.5 * 10 of anti-mycotic agent
61.9 * 10
72.7 0.07 0.12 born of the same parents bacterium King B
*: the colony counts actinomycetes that high-visibility pigment forms contain 7.1 * 10 of anti-mycotic agent
82.4 * 10
80.3 6.5 1.5
The albumin filamentous fungus contains the PD 1.1 * 10 of paraxin
81.6 * 10
81.5
The microbe population (every 1g root) that table 15-2 root face is lived
B/F
The present invention improves dosage organic improvement dosage alive
The 0% 4% 0% 4% aerobic little lifes of thing ratio microorganism nutrient agar contain 1.7 * 10 of anti-mycotic agent
82.7 * 10
81.6 the aerobic little life of 35.4 18.0 thing SCD contains 4.8 * 10 of anti-mycotic agent
88.8 * 10
81.8 100.0 58.7 thing albumin brood cell bars contain 2.2 * 10 of anti-mycotic agent
51.5 * 10
66.8 0.05 0.1 Pseudomonas SCD gram-negative contains 3.4 * 10 of anti-mycotic agent
74.0 * 10
71.2 7.1 2.7 property microorganism CVT gram-negatives contain 2.0 * 10 of anti-mycotic agent
76.6 * 10
73.3 4.2 4.4 property microorganism Columbia CNA fluorescence vacations singly contain 1.9 * 10 of anti-mycotic agent
52.2 * 10
611.6 0.04 0.15 born of the same parents bacterium * King B
*: the colony counts actinomycetes that high-visibility pigment forms contain 4.2 * 10 of anti-mycotic agent
71.3 * 10
83.0 8.8 8.7
The albumin filamentous fungus contains the PD 4.8 * 10 of paraxin
61.5 * 10
73.1 the fresh weight of root (g)/plant 13.2g 18.8g
Table 15 shows: compare with contrast (0% modifying agent), the microorganism that belongs to Bacillus and Pseudomonas fluorescens has increased by 2.5 and 2.7 times respectively, and actinomycetes have descended and fungi has only increased by 1.4 times.In the rhizoplane micropopulation, to compare with contrast (0% modifying agent), the microorganism that belongs to Bacillus and Pseudomonas fluorescens has increased by 6.8 and 11.6 times respectively, and actinomycetes and fungi have only increased by 3.0 and 3.1 times respectively.The remarkable breeding of useful micropopulation in root region soil and rhizoplane sees it also is tangible from B/F (prokaryotic organism number/fungi number separately) value.Increased because the fresh weight of root relies on adding modifying agent of the present invention, promoted plant-growth effectively so also prove this modifying agent.
Embodiment 5 rhizosphere microflora modifying agents (5)
Mensuration is to the effect of this modifying agent of the micropopulation of Shimonita leek.Used soil is at the Tomioka of Japan Town, and the peasant's of Gunma Prefecture soil, Shimonita leek are planted in and contain or do not contain in the soil of modifying agent 80kg/10a of the present invention.Experimental technique is identical with embodiment 2 and 4.On the experiment soil, observe the appearance that its growth stops.The catch an illness percentage ratio of plant, catch an illness index and residual branch loss ratio are shown in table 16.
The table 16 growth index of catching an illness of plant that stops to catch an illness
aResidual branch loss ratio
The calculating of index (DI) a) is caught an illness as: DI=∑ (n * SI)/4 * N * 100 in the soil that percentage ratio contrast soil 20% 50 2% contains this modifying agent 0% 0 0%
SI (severe degree that is hit index) wherein, 0 (healthy plant) is to the integer of 4 (dead plant); N=shows the plant number of identical SI value; N, the sum of the plant of observation.
Four kinds of Shimonita leeks are optionally picked out from every group, measure their weight immediately.The result is presented at table 17.Be determined at the microbial numbers of the work in non-root region soil and the rhizoplane in addition.The result is presented in the table 18.The weight of table 17 Shimonita leek
Gross weight (g) root fresh weight (g) root fresh weight (g) contrast 1 283.05 272.38 10.67
2????334.70????309.86????24.84
3????306.24????292.14????14.10
4????303.16????286.50????16.66
Average 306.79 290.22 16.57
Standard deviation 13.43 5.23
100% 100% 100% modifying agents 1 380.98 368.53 12.45 of the present invention
2?????321.26????308.33????12.93
3?????407.64????382.21????25.43
4?????364.45????356.12????8.33
Average 368.58 353.80 14.79
Standard deviation 31.37 27.83 6.40 modifying agents of the present invention/contrast 120% 122% 89%
Table 18-1 Shimonita leek soil (1996,11,13)
Non-root region soil live microorganism amount (every 1g dry ground earth)
B/F
The aerobic little life of this improvement of this improvement dosage dosage microorganism nutrient agar 0% 80kg/10a, 0% 80kg/10a contains 2.0 * 10 of anti-mycotic agent
81.4 * 10
8The aerobic little life of 200 538 thing SCD contains 3.5 * 10 of anti-mycotic agent
83.6 * 10
8350 1385 thing albumin bacilluss contain 5.0 * 10 of anti-mycotic agent
76.5 * 10
650 25 belong to the SCD gram-negative contains 6.7 * 10 of anti-mycotic agent
73.0 * 10
767 115 property microorganism CVT gram sun contain 1.3 * 10 of anti-mycotic agent
85.2 * 10
6130 20 property microorganism Columbia CNA fluorescence vacations singly contain 2.5 * 10 of anti-mycotic agent
68.7 * 10
63 33 born of the same parents bacterium * King B
*: the colony counts actinomycetes that high-visibility pigment forms contain 2.7 * 10 of anti-mycotic agent
71.0 * 10
727 38
The albumin filamentous fungus contains the PD 1.0 * 10 of paraxin
62.6 * 10
5
Table 18-2
Sticking root region soil grain live microorganism amount (the bright root of every 1g)
B/F
The aerobic little life of this improvement of this improvement dosage dosage microorganism nutrient agar 0% 80kg/10a, 0% 80kg/10a contains 4.1 * 10 of anti-mycotic agent
71.3 * 10
8The aerobic little life of 111 2281 thing SCD contains 1.6 * 10 of anti-mycotic agent
82.2 * 10
8432 3860 thing albumin bacilluss contain 3.3 * 10 of anti-mycotic agent
68.7 * 10
59 15 belong to SCD gram sun contains 1.7 * 10 of anti-mycotic agent
72.1 * 10
746 368 property microorganism CVT gram-negatives contain 1.3 * 10 of anti-mycotic agent
89.8 * 10
7351 1719 property microorganism Columbia CNA fluorescence vacations singly contain 1.1 * 10 of anti-mycotic agent
77.6 * 10
630 133 born of the same parents bacterium * King B
*: the colony counts actinomycetes that high-visibility pigment forms contain 5.6 * 10 of anti-mycotic agent
65.3 * 10
615 93
The albumin filamentous fungus contains the PD 3.7 * 10 of paraxin
55.7 * 10
4
On non-root region soil and the rhizoplane, several microbe populatioies that add this modifying agent low than on the soil that does not add this modifying agent.In non-root region soil, pass through to add modifying agent of the present invention, the aerobic microorganism in the relevant soil, fluorescent pseudomonas microbial, actinomycetes B/F value is high relatively, and these microorganisms also are high to the fungi ratio.Relevant in rhizoplane have aerobic microorganism, the bacillus microorganism belonging to genus, and any B/F value in Pseudomonas fluorescens and the actinomycetes is all obviously high, has increased the ratio of these microorganisms to fungi significantly by adding this modifying agent.In containing the soil of modifying agent of the present invention, the B/F value is than the B/F value height (except the microorganism of Bacillus) especially in non-root region soil in rhizoplane.
Above result shows: by adding modifying agent of the present invention, and the beginning that has stoped growth to stop, the fresh weight of leek has increased by 20%.And this modifying agent is more obvious to the effect of improved effect in rhizoplane than in non-soil of micropopulation.
According to the present invention, the modifying agent of rhizosphere microflora is provided, it can breed rhizosphere microflora (useful micropopulation) highlightedly in long-time.
Modifying agent of the present invention can stop and suppress the material that plant disease and promoting simultaneously uses effect of fertilizer so that plant health ground grow.
Claims (1)
1, the modifying agent of rhizosphere microflora, it contains the melocol condenses by formula I representative as active substance:
X-(NH
2-CONH-CH
2)
n-Y????(I)
Wherein X be hydrogen atom or-CH
2OH, Y are-NHCONH
2Or-OH and n are 4 or bigger integer.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2562596 | 1996-02-13 | ||
| JP25625/96 | 1996-02-13 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1165800A true CN1165800A (en) | 1997-11-26 |
Family
ID=12171064
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 97104966 Pending CN1165800A (en) | 1996-02-13 | 1997-02-13 | Improver of rhizosphere microflora |
Country Status (3)
| Country | Link |
|---|---|
| CN (1) | CN1165800A (en) |
| AU (1) | AU1266997A (en) |
| NL (1) | NL1005259C2 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103242201A (en) * | 2013-04-26 | 2013-08-14 | 中科院广州化学有限公司 | Preparation method of urea formaldehyde intermediate dimethylol urea |
| CN111334458A (en) * | 2020-04-13 | 2020-06-26 | 江苏省农业科学院 | Biocontrol actinomycetes and application thereof in prevention and control of ginger stem basal rot or soybean epidemic disease |
-
1997
- 1997-02-12 NL NL1005259A patent/NL1005259C2/en not_active IP Right Cessation
- 1997-02-12 AU AU12669/97A patent/AU1266997A/en not_active Abandoned
- 1997-02-13 CN CN 97104966 patent/CN1165800A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103242201A (en) * | 2013-04-26 | 2013-08-14 | 中科院广州化学有限公司 | Preparation method of urea formaldehyde intermediate dimethylol urea |
| CN103242201B (en) * | 2013-04-26 | 2015-06-10 | 中科院广州化学有限公司 | Preparation method of urea formaldehyde intermediate dimethylol urea |
| CN111334458A (en) * | 2020-04-13 | 2020-06-26 | 江苏省农业科学院 | Biocontrol actinomycetes and application thereof in prevention and control of ginger stem basal rot or soybean epidemic disease |
| CN111334458B (en) * | 2020-04-13 | 2021-08-31 | 江苏省农业科学院 | Biocontrol actinomycetes and application thereof in prevention and control of ginger stem basal rot or soybean epidemic disease |
Also Published As
| Publication number | Publication date |
|---|---|
| NL1005259C2 (en) | 1999-04-23 |
| NL1005259A1 (en) | 1997-12-10 |
| AU1266997A (en) | 1997-08-21 |
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