CN1876805A - Pseudomonas RRj228 helpful for crop growth and microbe preparation containing the same - Google Patents
Pseudomonas RRj228 helpful for crop growth and microbe preparation containing the same Download PDFInfo
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- CN1876805A CN1876805A CNA2005100750367A CN200510075036A CN1876805A CN 1876805 A CN1876805 A CN 1876805A CN A2005100750367 A CNA2005100750367 A CN A2005100750367A CN 200510075036 A CN200510075036 A CN 200510075036A CN 1876805 A CN1876805 A CN 1876805A
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
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Abstract
The invention relates the pseudomonas RRj228, which is used to replace fertilizer to promote the plant growth. The invention also relates the secondary metabolite, environment affinity microbiological agent, which is produced by pseudomonas RRj228.
Description
Technical field
The present invention relates to that (this bacterial strain is deposited in Korea S agricultural culture collection center (KACC) on June 11st, 2003, and deposit number is KACC 91052 by the pseudomonas RRj228 (Pseudomonas sp.RRj228) of No. 1 sequence representative; Again be preserved in Korea S typical case culture collection center (KCTC) on May 27th, 2005, deposit number is KCTC 10812BP), in the crop planting process, this bacterial strain replaces chemical fertilizer to promote plant-growth inside and outside root system of plant by growing surely.The invention still further relates to and contain pseudomonas RRj228 or by the environment affinity microbial preparation of the secondary metabolites of its generation.
Environment affinity microbial preparation according to the present invention by contain pseudomonas RRj228 or can reduce by the secondary metabolites of its generation chemical fertilizer usage quantity, stablize crop yield, protection water quality and edatope and improve the health of soil ecosystem.
Background technology
Fertilizer is a kind of important agriculture goods and materials of decision crop yield, but it as the factor inferior of crop growth can cause crops quality degenerations, river eutrophication, groundwater pollution, soil nutrient is unbalance and the output reduction.Therefore, the soil microorganisms for promoting the protection of sustainable development of agriculture and agricultural environment, must rationally use to have the function that promotes plant-growth and helping the surging growth of farm crop substitutes chemical fertilizer.
Do not form symbiotic relationship and can promote the short endophytic bacteria (Plant growth-promoting rhizobacteria) of plant rhizosphere of plant-growth (PGPR) to name by people such as Kloepper in 1980 with host plant, it comprises Azospirillum (Azospirrilum sp.) (Okon, 1985), Pseudomonas (Kloepper etc., 1980), bacillus (Backman etc., 1994) etc.The plant-growth that is called as PGPR promote microorganism have multiple function as the nitrogen in the fixing atmosphere, produce plant-growth and promote hormone, promotion nutrition absorption (Hallmann etc., 1997) and avoid plant to be subjected to disease invasion and attack (Pleban etc., 1995), therefore help plant-growth.
People such as Ward point out in 1992 annual report roads to classify and this microbe species of identifying account for live in all categories in the soil less than 5%, and people such as Torisvik nineteen ninety report point out in soil, to exist many not by the microorganism of artificial culture.Therefore, also have the more microorganism of finding than in the past with excellent functions in soil, this causes the follow-up study to them thus.
In addition, confirmed to inoculate different plant-growths and promoted microorganism can make rice yield improve 19-60% (Baldani, 2000), and can improve tomato (Kloepper and Schroth, 1981), beans (Polonenko etc., 1987; And Dashti etc., 1998), the output of wheat (Freitas and Germida, 1990) and beet (Suslow and Schrith, 1982).Plant-growth promotes that application of microorganism possesses some special knowledge.
Therefore, inventor of the present invention separates in the research soil microorganisms and has identified the pseudomonas RRj228 that can grow surely inside and outside the rice plants root system, and finds that this bacterial strain can promote crop growth, therefore alternative chemical fertilizer.
Summary of the invention
The purpose of this invention is to provide the pseudomonas RRj228 by No. 1 sequence representative, in the crop planting process, this bacterial strain replaces chemical fertilizer to promote plant-growth inside and outside root system of plant by growing surely.The present invention also aims to provide and contain pseudomonas RRj228 or by the environment affinity microbial preparation of the secondary metabolites of its generation.
The invention provides the pseudomonas RRj228 by No. 1 sequence representative, in the crop planting process, this bacterial strain replaces chemical fertilizer to promote plant-growth inside and outside root system of plant by growing surely.
Pseudomonas RRj228 is a kind of soil bacteria of being found by the present inventor, under field conditions (factors), this bacterium grows surely inside and outside root system of plant, and the secretion plant-growth promotes material, and suppresses the growth of multiple pathogenic agent by the antagonistic action to plant pathogenic fungi and bacterium.
The Gram-negative shaft-like thermophilic bacteria of pseudomonas RRj228 of the present invention in 28 ± 2 ℃ PDA (potato dextrose agar) substratum, cultivating.
The effect of using pseudomonas RRj228 of the present invention to obtain is as follows:
When 1) microbial preparation that contains this bacterial strain when use is as the farm crop inoculum,,, also can improve crop yield although therefore use a spot of chemical fertilizer because pseudomonas RRj228 of the present invention can provide outstanding nutrient supply and promote plant-growth.
2) the pseudomonas RRj228 of the present invention substitute that is chemical fertilizer has use range widely to farm crop, as can be used for paddy rice, capsicum and cucumber.
3) when pseudomonas RRj228 of the present invention is inoculated in the farm crop, this bacterial strain can be by suppressing the harm that plant pathogenic microorganisms in the soil is helped crop growth and avoided pathogenic agent.
4) pseudomonas RRj228 of the present invention can be by great expression in the farm crop that inoculate by growing surely in farm crop root system inside.
5) according to the present invention, except the effect of alternative chemical fertilizer, by will also producing more value from the plant-growth promotion of pseudomonas RRj228 nutrient solution or the secondary metabolites that the cause of disease growth-inhibiting substance separates and purifying obtains.
6) owing to the impossible contaminate environment of pseudomonas RRj228 of the present invention, thus water quality and edatope can be protected, and improve the health of soil ecosystem.
The present invention also provides the environment affinity microbial preparation of the secondary metabolites that contains pseudomonas RRj228 or its generation.
Can use method well known in the art that microbial preparation of the present invention is mixed with plant growth promoter or pathogenic growth inhibitor, but the present invention is not limited thereto.For substituting chemical fertilizer, microbial preparation of the present invention preferably is mixed with the organic fertilizer that is used to promote plant-growth.
When microbial preparation of the present invention was the plant growth promoter of dry powder or liquid fertilizer shape, the pseudomonas RRj228 content in per 1 gram dry powder or 1 milliliters of liquid was 105-107.
When microbial preparation of the present invention was the cause of disease growth inhibitor of dry powder or liquid fertilizer shape, the pseudomonas RRj228 content in per 1 gram dry powder or 1 milliliters of liquid should surpass 107.In this case, it is characterized in that this bacterial strain content is than the bacterial strain content height in the plant growth promoter.
Environment affinity microbial preparation according to the present invention can reduce by containing pseudomonas RRj228 chemical fertilizer consumption, stablize crop yield, protection water quality and edatope and improve the health of soil ecosystem.
Pseudomonas RRj228 can produce becomes suitable microbial preparation, and said preparation can be inoculated in farm crop such as paddy rice, capsicum and the cucumber.
Biological preservation
RRj228 bacterial strain of the present invention is named as pseudomonas RRj228, and being deposited in the Korea S agricultural culture collection center (KACC) that is attached to Developing Rural Office, ,Korea/Agricultural biotechnologies institute of country on June 11st, 2003, deposit number is KACC 91052.
On May 27th, 2005, preservation is to Korea S typical case culture collection center (KCTC) again for the RRj228 quilt, and deposit number is KCTC10812BP.
Description of drawings
Fig. 1 represents the flat board of pseudomonas RRj228 culture;
Fig. 2 a and 2b are the electromicroscopic photographs of pseudomonas RRj228;
Fig. 3 is the photo of growing surely in the pseudomonas RRj228 of roots of plants inside.
Embodiment
The present invention can promote active relevant embodiment to embody with plant-growth by the separating of following and pseudomonas RRj228, evaluation, pseudomonas RRj228 separates from the soil of St. Petersburg, Russia (St.Petersburg), and it also is one of isolated 172 kinds of bacterial strains from Korea S and Russian soil.Yet, provide these embodiment just to be used for explaining, can not be considered to limitation of the scope of the invention, appended claim has been done appropriate description to scope of the present invention.
Embodiment 1
The separation of soil microorganisms RRj228
The separation method of soil microorganisms RRj228 is as follows.
As shown in Figure 1, a rice paddy seed of sprouting in aseptic culture medium is transferred in the 0.6% agarose substratum.0.5 gram pedotheque (from St. Petersburg, Russia) is seeded in the centimeters apart from rice root 1-2, then substratum was cultivated 48 hours at 28 ℃.With root pulverize make it to form suspension after, this suspension is coated on the LC substratum (this substratum contains microbial culture with Tryptones 10 grams, yeast extract 5 grams, MgSO
47H
21 liter of O 8 gram, 1 milliliter of 1M Tris and distilled water), with isolated strains.
Embodiment 2
The evaluation of soil microorganisms RRj228
A. physio-biochemical characteristics
The physio-biochemical characteristics of RRj228 bacterial strain are presented in the following table 1.
Described RRj228 bacterial strain is shaft-like, and size is 2.0-3.0 * 0.5-0.7 micron, and it has flagellum shown in Fig. 2 a and 2b.When this bacterial strain is cultivated 24 hours in the PDB substratum after, can form yellow reddish bacterium colony.Described RRj228 bacterial strain is a Gram-negative bacteria, carries out aerobic metabolism and has catalase and oxidase activity.When the carbon source that adds substratum is pectinose, fructose, semi-lactosi, glucose, N.F,USP MANNITOL, sucrose, glycerine or wood sugar, this bacterial strain can produce acid, and when the carbon source that adds substratum was lactose, dextrin, ramnose, Sorbitol Powder, maltose or melampyrum, this bacterial strain can produce alkali.In addition, as shown in Figure 3, the feature of RRj228 bacterial strain of the present invention is to be present in the root system of plant outside also to grow surely in root system inside.
[table 1]
| Classification | Characteristic | Classification | Characteristic | ||
| Strain morphology | Shaft-like | Bacterial strain size (micron) | 2.0-3.0×0.5-0.7 | ||
| Flagellum | Have | Gemma | Do not have | ||
| Gramstaining | - | Colony colour on the PDA substratum | Yellow omiting redly | ||
| Aerobic metabolism | + | Anaerobic metabolism | - | ||
| Catalase activity | + | Oxidase activity | + | ||
| The gelatin hydrolysis activity | + | Lipase activity | - | ||
| The starch hydrolytic activity | - | The casein hydrolysis activity | + | ||
| Use the reaction of carbon source | Glucose | Form acid | Use the reaction of carbon source | Sucrose | Form acid |
| Pectinose | Form acid | Maltose | Form alkali | ||
| Wood sugar | Form acid | N.F,USP MANNITOL | Form acid | ||
| Lactose | Form alkali | Sorbyl alcohol | Form alkali | ||
| Semi-lactosi | Form acid | Melampyrum | Form alkali | ||
| Fructose | Form acid | Glycerine | Form acid | ||
| Ramnose | Form alkali | Dextrin | Form alkali | ||
B. sequential analysis
In order to analyze the RRj228DNA sequence, adopt 16S rDNA sequence analysis method.At first,, product is inserted in the pGEMT-T carrier, use automatic dna sequencer ABI3100 (Applied Biosystem, the U.S.) to carry out dna sequence analysis then with PCR (polymerase chain reaction) amplification 16S rDNA part.The fD1 (5 '-agagtttgatggctcag-3 ') of No. 2 sequences and the rP2 of No. 4 sequences (5 '-acggctaccttgttacgactt-3 ') are used in PCR reaction.In addition, use pUC/M13 forward primer (5 '-gttttcccagtcacgac-3 ': No. 4 sequences) and reverse primer (5 '-gcggataacaatttcacacagg-3 ': No. 5 sequences) react two end regions of analyzing above-mentioned primer, and use 4 primers to react to analyze the dna sequence dna of inside.
According to intestinal bacteria (Escherichia coli) 16S rDNA sequence (GenBank J01695 number), the 311-330 zone of each dna sequence dna use 16SP-1 (5 '-gccacactggaactgagacac-3 '), the 684-703 zone of 16SP-2 (5 '-tgtagcg gtgaaatgcgtg-3 '), the 1228-1247 zone of the 944-963 zone of 16SP-3 (5 '-ggagcatgtgt tattcg-3 ') and 16SP-4 (5 '-ctacacacgtgctacaatgg-3 ').
No. 1 sequence is represented the 16S rDNA sequence of RRj228 bacterial strain.Homology between the dna sequence dna in this bacterial strain and the GenBank database is discovered, the homology of this bacterial strain and many Pseudomonas members such as Pseudomonas chlororaphis (Pseudomonas chlororaphis), gauffer pseudomonas (P.Corrugata) and P.tolaasii reaches 99%, thus this bacterial strain is classified as Pseudomonas.
Therefore, RRj228 bacterial strain of the present invention is named as pseudomonas RRj228.
EXPERIMENTAL EXAMPLE 1
The plant-growth facilitation effect of pseudomonas RRj228
In order to detect the plant-growth facilitation effect of pseudomonas RRj228, corn seedling is experimentized.Corn root was immersed in these strain culture suspension 1.5 hours for terminal 3 millimeters, and other corn root immerses in aseptic culture fluid in contrast.Corn seedling is placed on the wet filter paper in the plastic box, with filter paper the root end at start line place is built then, cultivated 24 hours for 28 ℃.Measure the length of the root of new elongation, repeat 10 times, the corn growth facilitation effect of described RRj228 bacterial strain is presented in the following table 2.
[table 2]
The corn growth facilitation effect of RRj228 bacterial strain
| Bacterial strain | Corn growth (with the untreated % that compares) | |
| 1∶50 | 1∶100 | |
| RRj228 | -26 | +13.0 |
| Golden yellow bacillus (Chryseobacterium sp.) KR044 | 0 | +8 |
| Genus bacillus (Bacillus sp.) KR091 | 0 | 0 |
As shown in table 2, with stoste (4.5 * 10
9The c.f.u/ milliliter) with dilution in 1: 50 and when inoculating, the growth level of pseudomonas RRj228 of the present invention has reduced by 26%, and with stoste with dilution in 1: 100 and when inoculating, its growth level has improved 13%.Therefore, bacterial strain of the present invention is more effective when inoculating with low concentration.
EXPERIMENTAL EXAMPLE 2
The soil disease antagonistic action of pseudomonas RRj228
Use following method to detect the soil disease antagonistic action of pseudomonas RRj228.At first, in PDA, inoculate pathogenic fungi and bacterium respectively, then nutrient solution is transferred in the petri diss and made it and solidify.In each flat board, do a radius and be 4 millimeters well, with the pseudomonas RRj228 of 100 microlitres in the PDA liquid nutrient medium 28 ℃ cultivated 5 days, be inoculated into then in this well.Flat board was placed 5 days down at 28 ℃, detected the growth-inhibiting scope of PGPR then, this result is presented in the following table 3.With 5 milliliter (1 * 10
6Individual spore/milliliter) fungi and 5 milliliter (1 * 10
8The cfu/ milliliter) bacterium mixes with 250 milliliters of PDA substratum respectively and is incorporated in 45 ℃ of vibrations, and each petri diss is respectively got 25 milliliters of coated plates of solution, triplicate.
[table 3]
The RRj228 bacterial strain suppresses the growth scope of plant pathogenic fungi and bacterium
*(suppressing growth scope Φ mm)
| Bacterial strain | Plant pathogenic fungi | Plant pathogenetic bacteria | ||
| Phytophthora capsici (Phytophthora capsici) | Pythium spp (Pythium) | Carrot soft rot erwinia (Erwinia carotovora) 3391 | Pseudomonas syringae (Pseudomonas syringiae) 8300 | |
| RRj228 | 49.3±6.3 | 57.3±6.3 | 56.0±1.2 | 50.0±4.6 |
*Inoculate the pathogenic growth of this bacterial strain after 5 days and suppress scope
The result shows that RRj228 bacterial strain of the present invention shows very strong growth inhibitory effect to Phytophthora capsici, pythium spp, carrot soft rot erwinia and Pseudomonas syringae.
EXPERIMENTAL EXAMPLE 3
Pseudomonas RRj228 competition enters the relative capacity of plant root
For studying the relative capacity that pseudomonas RRj228 and fluorescent pseudomonas (Pseudomonasfluorescens) WCS365 competition enters plant root, two kinds of bacterial strains are inoculated into simultaneously after the root of paddy rice (Kumo paddy rice) and radish (Saxa nova), detect pseudomonas RRj228 and account for the per-cent of growing all bacterial strains in root surely, the result shows in following table 4.
[table 4]
Pseudomonas RRj228 and fluorescent pseudomonas WCS365 competition enter the relative capacity of plant root
| Bacterial strain | Density in the root (105cfu/ centimetre. root) | Occupy percentage (%) in the root | ||
| Paddy rice | Radish | Paddy rice | Radish | |
| Pseudomonas RRj228 | 7.90(1,975) | 72.5(145) | 95.2 | 99.3 |
| Fluorescent pseudomonas WCS365 | 0.40(100) | 0.5(100) | 4.8 | 0.7 |
EXPERIMENTAL EXAMPLE 4
The effect of inoculation pseudomonas RRj228 in farm crop
The nutrient solution of the pseudomonas RRj228 of the present invention that routine is cultivated is inoculated on paddy rice, capsicum, cucumber and the tomato, and the result is presented in the following table 5.
[table 5]
| Farm crop | Output | Percentage increases (%) | |
| Not inoculation | Inoculation | ||
| Paddy rice (in Korea S's mountain plantation, potted plant 3 weeks) | 40.4 gram | 53.8 gram | 33.2 |
| Capsicum (in Korea S's Qingyang plantation, potted plant 4 weeks) | 376.8 gram | 408.7 gram | 8.5 |
| Cucumber (from the Dynasty cucumber) (in the body after planting the 40th day) | 4.25 gram | 5.16 gram | 21.4 |
| Tomato (from the Sunyoung tomato) (in the body after planting the 40th day) | 1.66 gram | 3.61 gram | 117.5 |
As shown in table 5, confirm that pseudomonas RRj228 makes that paddy growth has increased by 33.2%, chili growth has increased by 8.5%, cucumber growth has increased by 21.4%, tomato growth has increased by 17.5%, and pseudomonas RRj228 of the present invention can be used as microbial fertilizer and substitutes chemical fertilizer.
Because pseudomonas RRj228 of the present invention directly separates from plant (paddy rice) root, rather than the bacterial strain before resembling is isolating from soil, so this bacterial strain can suitably be grown surely in the root system of farm crop such as paddy rice and cucumber, therefore when with this inoculation to farm crop the time, it can be by stably growing self duplication in the farm crop root system and inside and outside root system surely, between the farm crop survival time, because rainwater or arid are difficult to make its density to reduce, so its effect of inoculation can keep for a long time.
Industrial usability
According to environment compatibility microorganism formulation of the present invention by using pseudomonas RRj228, can reduce chemical fertilizer use, stablize crop yield, protect water quality and soil environment and improve the health of soil ecosystem.
Sequence table
＜110〉Developing Rural Office, ,Korea (Republic of Korea represented by Rural Development Administration)
＜120〉help the pseudomonas of crop growth and contain the microbial preparation of this bacterial strain
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<151>2004-06-07
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＜213〉pseudomonas RRj228 (Pseudomonas sp.RRj228)
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gtagagagaa gcttgcttct cttgagagcg gcggacgggt gagtaatgcc taggaatctg 120
cctggtagtg ggggataacg tccggaaacg gacgctaata ccgcatacgt cctacgggag 180
aaagcagggg accttcgggc cttgcgctat cagatgagcc taggtcggat tagctagttg 240
gtgaggtaat ggctcaccaa ggcgacgatc cgtaactggt ctgagaggat gatcagtcac 300
actggaactg agacacggtc cagactccta cgggaggcag cagtggggaa tattggacaa 360
tgggcgaaag cctgatccag ccatgccgcg tgtgtgaaga aggtcttcgg attgtaaagc 420
actttaagtt gggaggaagg gtacttacct aatacgtgag tattttgacg ttaccgacag 480
aataagcacc ggctaactct gtgccagcag ccgcggtaat acagagggtg caagcgttaa 540
tcggaattac tgggcgtaaa gcgcgcgtag gtggttcgtt aagttggatg tgaaatcccc 600
gggctcaacc tgggaactgc atccaaaact ggcgagctag agtatggtag agggtggtgg 660
aatttcctgt gtagcggtga aatgcgtaga tataggaagg aacaccagtg gcgaaggcga 720
ccacctggac atgatactga cactgaggtg cgaaagcgtg gggagcaaac aggattagat 780
accctggtag tccacgccgt aaacgatgtc aactagccgt tgggagcctt gagctcttag 840
tggcgcagct aacgcattaa gttgaccgcc tggggagtac ggccgcaagg ttaaaactca 900
aatgaattga cgggggcccg cacaagcggt ggagcatgtg gtttaattcg aagcaacgcg 960
aagaacctta ccaggccttg acatccaatg aactttccag agatggattg gtgccttcgg 1020
gaacattgag acaggtgctg catggctgtc gtcagctcgt gtcgtgagat gttgggttaa 1080
gtcccgtaac gagcgcaacc cttgtcctta gttaccagca cgttatggtg ggcactctaa 1140
ggagactgcc ggtgacaaac cggaggaagg tggggatgac gtcaagtcat catggccctt 1200
acggcctggg ctacacacgt gctacaatgg tcggtacaga gggttgccaa gccgcgaggt 1260
ggagctaatc ccataaaacc gatcgtagtc cggatcgcag tctgcaactc gactgcgtga 1320
agtcggaatc gctagtaatc gcgaatcaga atgtcgcggt gaatacgttc ccgggccttg 1380
tacacaccgc ccgtcacacc atgggagtgg gttgcaccag aagtagctag tctaaccttc 1440
gggaggacgg ttaccacggt gtgattcatg actggggtga agtcgtaaca aggtagccgt 1500
a 1501
<210>2
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＜213〉artificial sequence
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＜223〉be used to the to increase pcr amplification primer fD1 of pseudomonas RRj228 gene
<400>2
agagtttgat ggctcag 17
<210>3
<211>21
<212>DNA
＜213〉artificial sequence
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＜223〉be used to the to increase pcr amplification primer rP2 of pseudomonas RRj228 gene
<400>3
acggctacct tgttacgact t 21
<210>4
<211>17
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＜213〉artificial sequence
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＜223〉be used to the to increase pcr amplification forward primer of pUC/M13 of pseudomonas RRj228 gene
<400>4
gttttcccag tcacgac 17
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＜213〉artificial sequence
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＜223〉be used to the to increase pcr amplification reverse primer of pUC/M13 of pseudomonas RRj228 gene
<400>5
gcggataaca atttcacaca gg 22
Claims (7)
1, a kind of pseudomonas RRj228 (Pseudomonas sp.RRj228), this bacterial strain contains the rDNA sequence by No. 1 sequence representative, and the deposit number of this bacterial strain is KCTC10812BP.
2, a kind of microbial preparation, this microbial preparation contain pseudomonas RRj228 as claimed in claim 1 as effective constituent.
3, according to the microbial preparation of claim 2, wherein, described preparation is for substituting the organic fertilizer of chemical fertilizer.
4, according to the microbial preparation of claim 2, wherein, described preparation is used to promote plant-growth.
5, according to the microbial preparation of claim 2, wherein, described preparation is used to suppress pathogenic growth.
6, according to the microbial preparation of claim 2, wherein, described preparation also contains the secondary metabolites of pseudomonas RRj228 as effective constituent.
7, a kind of soil management or plant cultivation method, this method is by with handling soil as any described microbial preparation among the claim 2-6 or plant carries out soil management or plant cultivation.
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|---|---|---|---|
| KR1020040041457A KR100646597B1 (en) | 2004-06-07 | 2004-06-07 | 228 Pseudomonas sp. RRj228 helping the growth of crops and the microbial agent containing the same |
| KR1020040041457 | 2004-06-07 |
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|---|---|
| CN1876805A true CN1876805A (en) | 2006-12-13 |
| CN100417719C CN100417719C (en) | 2008-09-10 |
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| WO2018128985A1 (en) * | 2017-01-03 | 2018-07-12 | Monsanto Technology Llc | Microbial compositions and methods |
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| KR100973168B1 (en) | 2008-05-08 | 2010-07-30 | 충북대학교 산학협력단 | Pseudomonas corrugata mutants promoting plant growth at low temperature and method for promoting plant growth using the same |
| KR101153462B1 (en) | 2010-01-08 | 2012-06-27 | 대한민국 | A New bacteria to enhance rice production |
| KR101972379B1 (en) * | 2018-11-08 | 2019-04-25 | 김경현 | A method for managing golf lawns at low temperatures |
| KR102324981B1 (en) * | 2020-06-11 | 2021-11-12 | 연세대학교 원주산학협력단 | Novel Pseudomonas Fluorescens Strain that Induces Resistance to Drought Stress in Plant and Uses Thereof |
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| US6348193B1 (en) * | 1999-09-02 | 2002-02-19 | Board Of Trustees Of Michigan State University | Method for inhibiting pathogenic fungi and bacteria in plants using Pseudomonas aureofaciens |
| CN1234846C (en) * | 2003-05-22 | 2006-01-04 | 郑爱萍 | Strain of pseudomonas for promoting growth of paddy as well as preventing and curing sheath blight of rice |
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|---|---|---|---|---|
| WO2018128985A1 (en) * | 2017-01-03 | 2018-07-12 | Monsanto Technology Llc | Microbial compositions and methods |
| CN110300514A (en) * | 2017-01-03 | 2019-10-01 | 孟山都技术公司 | Microbial composite and method |
| CN110300514B (en) * | 2017-01-03 | 2023-04-07 | 孟山都技术公司 | Microbial compositions and methods |
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| Publication number | Publication date |
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| KR20050116297A (en) | 2005-12-12 |
| CN100417719C (en) | 2008-09-10 |
| KR100646597B1 (en) | 2006-11-23 |
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