KR102324981B1 - Novel Pseudomonas Fluorescens Strain that Induces Resistance to Drought Stress in Plant and Uses Thereof - Google Patents

Abstract

The present invention relates to a novel Pseudomonas fluorescens strain inducing resistance to drought stress of plants and uses thereof and, more specifically, to a Pseudomonas fluorescens DR397 strain inducing resistance to drought stress of plants, to a composition for inducing resistance to drought stress of plants including the same as an active ingredient, and to a method for inducing resistance to drought stress of plants using the same. The strain can be effectively utilized in sustaining and promoting plant growth even in a water-stressed environment by inducing resistance to drought stress of plants.

Description

Novel Pseudomonas Fluorescens Strain that Induces Resistance to Drought Stress in Plant and Uses Thereof

The present invention relates to a novel Pseudomonas fluorescens strain inducing resistance to drought stress of plants and uses thereof, and specifically, Pseudomonas fluorescens DR397 strain inducing resistance to drought stress of plants and active ingredients thereof To a composition for inducing resistance to drought stress of a plant comprising a, and a method for inducing resistance to drought stress of a plant using the same.

Stress conditions such as drought, salt and low temperatures cause great damage to agricultural crop production. In particular, drought is a serious factor that limits plant growth and crop yield among abiotic stresses. In the case of Korea, the damage to crops due to drought is greater because traditionally, agriculture that requires a lot of water, such as rice farming, is carried out. In addition, drought occurs every year due to the difference in precipitation by season, year, and region. According to data from the Ministry of Agriculture, Food and Rural Affairs in 2017, drought damage occurred in a total area of 71,225 hectares, 1.2 times the size of Seoul for five years since 2012. . The main damage cases are phenomena such as the dryness of paddy water and the withering of field crops, and most of the damage occurred in paddy fields and fields, the base of economic activity of farmers.

Recently, in order to prevent crop damage due to drought, the development of drought-resistant plants using genetic engineering technology is in progress. Korean Patent No. 1219013 discloses 'a drought-resistant gourd transformed with ABF3 gene and a manufacturing method thereof', and Korean Patent No. 1175102 discloses 'rice-derived OsABF2 gene and uses thereof'. However, there is a report that an allergic reaction may occur to the genetically engineered plant or food using the same, and many consumers have strong resistance to the genetically modified plant or food.

Accordingly, the present inventors have found that plants have resistance to drought without genetic manipulation, and as a result of searching for a new method to promote plant growth even in a water-scarce environment, a new method having the ability to induce drought tolerance among soil microorganisms The present invention was accomplished by discovering microorganisms.

An object of the present invention is to provide a Pseudomonas fluorescens DR397 strain that induces resistance to drought stress of plants.

In addition, an object of the present invention is to provide a composition for inducing resistance to drought stress of a plant comprising the strain or a culture thereof as an active ingredient.

Another object of the present invention is to provide a method for inducing resistance to drought stress in a plant, comprising the step of treating a plant or soil with the composition.

One aspect of the present invention provides a Pseudomonas fluorescens DR397 strain inducing resistance to drought stress of plants.

The Pseudomonas fluorescens DR397 strain was selected as the strain with the best ability to induce resistance to drought stress of plants as a result of the present inventors collecting and culturing microorganisms present in the soil, and the 16s rRNA sequence of the strain was analyzed This was confirmed to have 99% sequence homology with the existing Pseudomonas fluorescens strain, and was identified as Pseudomonas Fluorescens . The DR397 strain was deposited with the Korean Culture Center of Microorganisms on May 6, 2020 (accession number: KCCM12710P).

In one embodiment, the strain may have phosphoric acid solubilization, IAA production ability and nitrogen fixation ability. According to an embodiment of the present invention, the strain is excellent in activity than the existing known strains or other Pseudomonas spp. strains in terms of phosphoric acid solubilization, nitrogen fixing ability, IAA and siderophore production, and as a result of crop cultivation experiments, plant growth under drought conditions It was confirmed that it can be significantly promoted.

In one embodiment, the 16s rRNA nucleotide sequence of the strain may consist of the nucleotide sequence represented by SEQ ID NO: 1.

The strain is a siderophore production ability, ACC (1-aminocyclopropane-1-carboxylate synthase) deaminase (ACC deaminase) activity, acetoin (acetoin) as mycological properties helpful in promoting plant growth Performance, butanediol (butanediol) synthesizing ability, phenazine (phenazine) production ability and chitinase (chitinase) production ability may have more than one property from the group consisting of.

The resistance to drought stress may be to exhibit plant growth promoting activity in soil having a moisture content of 20 to 40% by weight, 25 to 35% by weight, or 30% by weight relative to the total weight of the soil. For example, it may be to exhibit plant growth promoting activity in soil having a soil moisture content of 20 to 40% by stopping the supply of moisture to the soil for 7 days.

Resistance to the drought stress may be to exhibit plant growth promoting activity in soil having an average temperature of about 10 to 30 °C, 10 to 25 °C, or 10 to 20 °C.

Resistance to the drought stress is a pH of 6.0 to 8.0, 6.5 to 8.0, 7.0 to 8.0, 6.0 to 7.5, 6.0 to 7.0, 6.5 to 7.5, 6.5 to 7.0, or 7.0 to 7.5 in a soil with plant growth promoting activity in soil it could be

The tolerance to drought stress may be optimized in Korean soil.

As used in the present invention, 'drought stress' refers to a state in which normal water supply necessary for plant growth is not achieved, and abiotic conditions that damage plant growth and survival (eg, exposure to dry conditions, lack of water) exposure to conditions).

As used herein, 'tolerance' or 'resistance' refers to the property of a plant to withstand environmental stress such as drought.

In addition, another aspect of the present invention provides a composition for inducing resistance to drought stress of a plant comprising the Pseudomonas fluorescens DR397 strain, or a culture thereof as an active ingredient.

Since the strain of the present invention increases the expression of various molecules inducing resistance to drought stress of plants, it is possible to sustain and promote plant growth even in a water-stressed environment. At this time, the strain has excellent phosphoric acid solubilization activity and IAA production activity, and has a nitrogen fixing ability to reduce nitrogen in the atmosphere to ammonia, so it can promote plant growth. According to an embodiment of the present invention, it was confirmed that the strain is excellent in the growth promoting effect of legumes such as peas, kidney beans, etc. than existing known strains or other Pseudomonas sp. strains. According to an embodiment of the present invention, the composition increases the growth rate of plants by 40%, 50%, 60%, 70%, 80%, 90%, or 100% compared to the case where resistance to drought stress is not induced. may be more than

In one embodiment, the composition comprises Pseudomonas fluorescens DR397 strain 1x10 ⁷ to 1x10 ¹¹ cfu/mL, 1x10 ⁸ to 1x10 ¹⁰ cfu/mL, or 1x10 ⁹ to 5x10 ⁹ cfu/mL, or a culture thereof may include. At this time, the culture may be a mixture of the strain and distilled water or TSB liquid medium (trypticase soy broth), but is not limited thereto.

Such a composition may be formulated in various forms, and specifically may be prepared in the form of dry powder or liquid fertilizer. Examples thereof may be liquids, granules, powders, emulsions, oil agents, wettable powders, coating agents, and the like, and in this case, a solvent or carrier may be added thereto.

In addition, another aspect of the present invention provides a method of inducing resistance to drought stress of a plant comprising the step of treating the composition to the plant or soil.

The above step is a process of treating the plant with the composition for inducing resistance to drought stress directly on the plant or on the surrounding soil.

The plant is not particularly limited as long as it is a plant that can be grown as a crop in the art, and for example, cereals such as wheat, rice, corn, sorghum, millet; It may be an edible crop such as legumes such as kidney beans, red beans, peas, peas, and soybeans, potatoes, sweet potatoes, taro, and potatoes such as hemp.

The treatment method of the composition may be applied to the surface of the soil around the plant, mixed or irrigated in the soil, sprayed or sprayed directly on the stem, leaf or branch of a plant, or sprayed or immersed in the seed of a plant. , but is not limited thereto.

The Pseudomonas fluorescens DR397 strain according to the present invention can be usefully used for sustaining and promoting plant growth even in a water scarce environment by inducing resistance to drought stress of plants.

1 shows a phylogenetic tree analysis result of Pseudomonas fluorescens DR397 strain according to an embodiment of the present invention.
Figure 2 shows the type and number of stress response-related genes possessed by the DR397 strain according to an embodiment of the present invention.
Figure 3 shows the results of crop cultivation using the DR397 strain culture according to an embodiment of the present invention. The control group of each condition treated only the medium without the strain, and the test group treated the DR397 strain solution to grow peas and kidney beans under conditions of insufficient water (dry condition) or sufficient water (non-drought condition). Then, by measuring the stem length, root length, total fresh weight and dry weight of each group, the increase rate compared to control (%) of the test group compared to the control group of each condition was calculated.

Hereinafter, the present invention will be described in more detail. However, these descriptions are provided for illustrative purposes only to help the understanding of the present invention, and the scope of the present invention is not limited by these illustrative descriptions.

1. Identification of strains

In June 2018, 10 g of samples were collected from the soil in the soybean cultivation area located in Wonju, Gangwon-do. 90 mL of sterile physiological saline was added to the soil sample and incubated with shaking at 150 rpm for 30 minutes. Thereafter, the culture was prepared by serial dilution. As a culture medium for strain culture, a TSA solid medium (Tryptic Soy Agar) (BD Bacto™) containing polyethylene glycol (PEG 6000) (-1.2 Mpa) at a concentration of 25% was prepared. 100 μl of the culture solution diluted in TSA solid medium was spread and cultured at 37° C. for 7 days. Thereafter, colonies were isolated from TSA solid medium and subcultured in fresh TSA solid medium to separate pure water. Functional tests were performed on 8 isolated strains.

2. Strain functional test

2-1. Measurement of Indole-3-Acetic Acid (IAA) Production

Each strain was inoculated at 1% in YEM (Yeast-extract-mannitol) liquid medium containing 1% tryptophan, and then cultured at 30°C and 120 rpm for 5 days. After centrifuging the culture solution at 13,000 rpm for 20 minutes, the supernatant was reacted with Salkowski's reagent (containing _{2 mL of 0.5M FeCl 3} , 49 mL of 70% perchloric acid and 49 mL of water) for 25 minutes to measure the absorbance at 530 nm. did.

2-2. Measurement of nitrogen fixing ability

25 mL of nitrogen-restricted medium (Burk's medium) and 2.5 mL (10 ⁴ CFU/mL) of each strain were injected into a 125 mL serum bottle that could be blocked from the outside and cultured. At this time, 10% of the gas in the head space was replaced with acetylene gas and cultured at 30° C. and 180 rpm for 6 hours. Then, the gas was collected, and the degree of reduction of acetylene to ethylene was measured using gas chromatography.

2-3. Measurement of P solubilization

Each strain was inoculated at 1% in PSB liquid medium and then cultured at 28°C and 120 rpm for 3 days. After centrifugation of the culture medium at 13,000 rpm for 20 minutes, 200 uL of the supernatant was reacted with 50 uL of vanadate-molybdate reagent for 30 seconds, and the absorbance was measured at 530 nm.

2-4. Measurement of siderophore production

100 μl of each strain was plated on CAS solid medium (chrome azurol sulfonate medium) and cultured at 28° C. for 48 days. Thereafter, it was determined whether sideropores were produced by checking the yellow rings formed around the colonies.

2-5. Confirmation of plant growth promotion under drought stress

Each strain was inoculated in TSB liquid medium, incubated at 28° C. until log phase, and centrifuged at 8,000 rpm for 20 minutes. Each centrifuged strain was washed twice in 1:10 diluted TSB liquid medium to prepare a strain solution. The strain solution was used as the test group, and only the TSB liquid medium diluted 1:10 was used as the control group.

Pea and kidney bean seeds were added to 2.5% sodium hypochlorite (NaClO), the surface was washed for 3 minutes, sterilized, and then washed with distilled water 5 times. 50 holes (each 73 cm ³ ea.) Mix 15 g of standard soil and 2 mL of strain solution or dilution medium (1x10 ⁹ cfu/mL) in each pot of the seedling tray and pour 1 sterilized seeds each. A total of 20 grains were sown each lip. Water was injected once a day for the first 10 days after sowing, and water was not provided for 7 days thereafter, and water was injected again every day after 7 days for cultivation. After 24 days, the growth rate of the test group compared to the control group was calculated by measuring the stem length and dry root weight of the cultivated peas and kidney beans.

The results of analyzing the IAA production, nitrogen fixation capacity, phosphorus solubilization and sideropore production measurements of 8 strains, and plant growth rate under drought stress conditions are shown in Table 1 below.

As a result, it was confirmed that the DR397-treated group produced IAA and siderophores, and not only had excellent nitrogen fixing ability and phosphorus solubilization compared to other strains, but also promoted both pea and kidney bean growth at a high level under drought stress conditions.

strain IAA production
(ug/mL) N fixed
(nmol/mg/hr) phosphorus solubilization
(ppm) side pore
production or not pea kidney bean Stem Length (%) root dry weight
(%) stem length
(%) root dry weight
(%) DR397 26.4 42.5 403.5 + 76.6 95.6 120.5 68.7 DR133 26.5 44.9 277.7 + 52.3 34.3 -20.2 21.7 DR205 72.9 26.4 397.3 + 74.3 156.5 194.6 51.3 DR208 12.9 21.5 332.5 + 18.1 152.2 147.8 5.8 DR312 16.3 21.7 345.4 + 21.3 178.3 135.9 47.6 DR408 22.9 27.7 418.8 + 5.1 178.3 180.3 60.3 DR822 28.9 23.6 285.5 + 3.8 52.2 114.5 71.3 DR048 28.4 65.5 216.4 + 61.4 15.9 146.4 5.9

3. Mycological properties and genetic analysis of DR397 strain

The DR397 strain was confirmed to be a motile, Gram-negative, non-spore bacilli, aerobic, producing a yellow-green fluorescent dye, and having an optimal culture temperature of 30 ~ 37 °C (mesophilic).

16s rRNA sequencing of strain DR397 was performed in the following way. After extracting the genomic DNA of the strain using the FastDNA™ SPIN Kit (MP Biomedicals), the concentration and purity of the extracted DNA were measured using the PicoGreen™ dsDNA quantitation kit. Then, the extracted DNA was amplified with a pair of primers. After purifying the amplified PCR product using the QIAquick PCR Purification Kit, 16S rRNA gene sequencing was performed. The 16s rRNA sequence of the DR397 strain was analyzed using NCBI Blast. The 16s rRNA sequencing results of the DR397 strain are shown in Table 2 below.

SEQ ID NO: division base sequence (5'>3') One DR397 16s rRNA AGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGATGAAGGGAGCTTGCTCCTGAATTCAGCGGCGGACGGGTGAGTAATGCCTAGGAATCTGCCTGGTAGTGGGGGACAACGTTTCGAAAGGAACGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGACCTTCGGGCCTTGCGCTATCAGATGAGCCTAGGTCGGATTAGCTAGTTGGTGAGGTAATGGCTCACCAAGGCGACGATCCGTAACTGGTCTGAGAGGATGATCAGTCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGTTGTAGATTAATACTCTGCAATTTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTCGTTAAGTTGGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCCAAAACTGGCGAGCTAGAGTATGGTAGAGGGTGGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACACCAGTGGCGAAGGCGACCACCTGGACTGATACTGACACTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCAACTAGCCGTTGGGAGCCTTGAGCTCTTAGTGGCGCAGCTAACGCATTAAGTTGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGCCTTGACATCCAATGAACTTTCCAG AGATGGATTGGTGCCTTCGGGAACATTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAACCCTTGTCCTTAGTTACCAGCACGTTATGGTGGGCACTCTAAGGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGGCCTGGGCTACACACGTGCTACAATGGTCGGTACAAAGGGTTGCCAAGCCGCGAGGTGGAGCTAATCCCATAAAACCGATCGTAGTCCGGATCGCAGTCTGCAACTCGACTGCGTGAAGTCGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCT 2 forward primer AGAGTTTGATCCTGGCTCAG 3 Reverse primer GGTTACCTTGTTACGACTT

In addition, whole genome analysis was performed in the following way. After genomic DNA was extracted using the PureLink® Genomic DNA Extraction mini kit, whole genome analysis was performed through the PacBio RSII platform (Pacific Biosciences Inc.). The long read sequence obtained through the analysis was assembled through the de-novo assembler HGAP3, and finally the sequence error was corrected using Pilon (v1.21). Then, each gene was analyzed using PGAP (Prokaryotic Genome Annotation Pipeline) provided by NCBI. In addition, the average similarity of each genome sequence was analyzed using EZBiocloud. Each gene was analyzed through Prokka software, and genes related to plant growth promotion were classified. Using NCBI Blast, the useful genes of the DR397 strain were compared with those of the existing strains to select genes showing high homology.

Phylogenetic analysis of strain DR397 was performed using NCBI Blast. For phylogenetic tree analysis, sequences obtained from 16s rRNA gene sequencing and whole genome sequencing and sequences extracted from the NCBI database were collected, and phylogenetic characteristic-based prediction analysis was performed on the collected sequences using the Maximum Likelihood method through MEGA7 software. For the phylogenetic tree, Kimura-2-parameter evolution model was used, and 1000 times of bootstrapping was performed to confirm reliability.

As a result, as shown in FIG. 1, the DR397 strain was classified as Pseudomonas fluorescens based on the 16s rRNA nucleotide sequence, and as shown in Table 3 below, the DR397 strain has 99 similarities with the existing Pseudomonas fluorescens strain. % was confirmed.

Comparative strain homology Identity Score Accession Pseudomonas fluorescens CCM 2115 99% 97.2% 2575 NR115715 Pseudomonas fluorescens IAM 12022 99% 96.7% 2536 NR043420 Pseudomonas ficuserectae JCM2400 99% 96.9% 2542 NR074797 Pseudomonas chlorophis DSM 50083 97% 97.8% 2579 NR044974 Pseudomonas putida ATCC 12633 97% 97.4% 2547 NR114479 Pseudomonas marginalis ATCC 10844 98% 97.1% 2547 NR112072

In addition, as shown in Figure 2, the DR397 strain was confirmed to have a variety of stress response-related genes. Through these results, it can be expected that the DR397 strain will also exhibit the effect of resistance to plant stress caused by water shortage.

The present inventors deposited the Pseudomonas fluorescens DR397 strain at the Korean Culture Center of Microorganisms on May 6, 2020 and were given an accession number KCCM12710P, and then used to confirm the plant growth promoting effect.

4. Confirmation of plant growth promoting effect in drought stress using DR397 strain

The DR397 strain was inoculated in TSB liquid medium and incubated at 28° C. until log phase, followed by centrifugation at 8,000 rpm for 20 minutes. The centrifuged strain was washed twice in 1:10 diluted TSB liquid medium to prepare a strain solution. The strain solution was used as the test group, and only the TSB liquid medium diluted 1:10 was used as the control group.

Pea and kidney bean seeds were added to 2.5% sodium hypochlorite (NaClO), the surface was washed for 3 minutes, sterilized, and then washed with distilled water 5 times. 50 holes (each 73 cm ³ ea.) Mix 15 g of standard soil and 2 mL of strain solution or dilution medium (1x10 ⁹ cfu/mL) in each pot of the seedling tray and pour 1 sterilized seeds each. A total of 20 grains were sown each lip. In the case of drought conditions, water was injected once a day for the first 10 days after sowing, and water was not provided for 7 days thereafter, and water was injected once a day for 7 days thereafter. In the case of non-drought conditions, after sowing, water was injected once a day for cultivation. The length, total fresh and dry weight of stems and roots of peas and kidney beans of each group grown for 24 days were measured, and the results are shown in Table 4.

growing conditions Root length (cm) Stem Length (cm) Raw weight (mg) dry weight (mg) pea drought
condition control 2.4±1.2 3.0±1.2 12.2±2.0 2.3±0.3 test group 6.0±2.0 5.3±1.3 28.2±12.1 4.5±1.3 rain drought
condition control 7.2±3.1 6.2±3.1 36.8±16.1 6.7±0.9 test group 8.1±2.6 7.6±1.2 51.0±8.0 6.9±1.6 kidney bean drought
condition control 12.3±2.2 11.7±8.3 53.3±34.3 17.7±14.6 test group 19.9±2.0 25.8±2.6 74.9±7.0 24.9±2.3 rain drought
condition control 19.4±2.3 30.9±4.0 108.5±21.9 37.16±7.3 test group 21.6±3.0 30.2±1.7 75.1±6.0 28.9±2.4

As a result, root length, stem length, fresh weight, and dry weight were all increased in the test group grown in the drought condition with insufficient water compared to the test group in the non-drought condition in which water was sufficiently supplied.

These results show that even if the DR397 strain competes with microorganisms already present in the soil, it can settle and work smoothly, and in that it can effectively promote plant growth in a water-stressed environment, the DR397 strain is resistant to drought stress of plants support to induce Furthermore, the DR397 strain is judged to have high potential for use as a plant growth promoter inducing resistance to drought stress of plants.

Korea Microorganism Conservation Center (Overseas) KCCM12710P 20200506

<110> UNIVERSITY INDUSTRY FOUNDATION, YONSEI UNIVERSITY WONJU CAMPUS <120> Novel Pseudomonas Fluorescens Strain that Induces Resistance to Drought Stress in Plant and Uses Thereof <130> PN200115 <160> 3 <170> KoPatentIn 3.0 <210> 1 <211> 1525 <212> RNA <213> Artificial Sequence <220> <223> DR397 16s rRNA <400> 1 agagtttgat catggctcag attgaacgct ggcggcaggc ctaacacat caagtcgagc 60 ggatgaaggg agcttgctcc tgaattcagc ggcggacggg tgagtaatgc ctaggaatct 120 gcctggtagt gggggacaac gtttcgaaag gaacgctaat accgcatacg tcctacggga 180 gaaagcaggg gaccttcggg ccttgcgcta tcagatgagc ctaggtcgga ttagctagtt 240 ggtgaggtaa tggctcacca aggcgacgat ccgtaactgg tctgagagga tgatcagtca 300 cactggaact gagacacggt ccagactcct acgggaggca gcagtgggga atattggaca 360 atgggcgaaa gcctgatcca gccatgccgc gtgtgtgaag aaggtcttcg gattgtaaag 420 cactttaagt tgggaggaag ggttgtagat taatactctg caattttgac gttaccgaca 480 gaataagcac cggctaactc tgtgccagca gccgcggtaa tacagagggt gcaagcgtta 540 atcggaatta ctgggcgtaa agcgcgcgta ggtggttcgt taagttggat gtgaaatccc 600 cgggctcaac ctgggaactg catccaaaac tggcgagcta gagtatggta gagggtggtg 660 gaatttcctg tgtagcggtg aaatgcgtag atataggaag gaacaccagt ggcgaaggcg 720 accacctgga ctgatactga cactgaggtg cgaaagcgtg gggagcaaac aggattagat 780 accctggtag tccacgccgt aaacgatgtc aactagccgt tgggagcctt gagctcttag 840 tggcgcagct aacgcattaa gttgaccgcc tggggagtac ggccgcaagg ttaaaactca 900 aatgaattga cggggggcccg cacaagcggt ggagcatgtg gtttaattcg aagcaacgcg 960 aagaacctta ccaggccttg acatccaatg aactttccag agatggattg gtgccttcgg 1020 gaacattgag acaggtgctg catggctgtc gtcagctcgt gtcgtgagat gttgggttaa 1080 gtcccgtaac gagcgcaacc cttgtcctta gttaccagca cgttatggtg ggcactctaa 1140 ggagactgcc ggtgacaaac cggaggaagg tggggatgac gtcaagtcat catggccctt 1200 acggcctggg ctacacacgt gctacaatgg tcggtacaaa gggttgccaa gccgcgaggt 1260 ggagctaatc ccataaaacc gatcgtagtc cggatcgcag tctgcaactc gactgcgtga 1320 agtcggaatc gctagtaatc gcgaatcaga atgtcgcggt gaatacgttc ccgggccttg 1380 tacacaccgc ccgtcacacc atgggagtgg gttgcaccag aagtagctag tctaaccttc 1440 gggaggacgg ttaccacggt gtgattcatg actggggtga agtcgtaaca aggtagccgt 1500 aggggaacct gcggctggat cacct 1525 <210> 2 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Forward primer <400> 2 agagtttgat cctggctcag 20 <210> 3 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> Reverse primer <400> 3 ggttaccttg ttacgactt 19

Claims (6)

Pseudomonas fluorescens DR397 strain (Accession No.: KCCM12710P) comprising a 16s rRNA base sequence consisting of the base sequence shown in SEQ ID NO: 1, and inducing resistance to drought stress of plants.
The method according to claim 1,
The strain is that having a phosphoric acid solubilization, IAA production capacity and nitrogen fixation capacity, the strain.
delete A composition for inducing resistance to drought stress of a plant comprising the strain of claim 1 or a culture thereof as an active ingredient.
5. The method according to claim 4,
The composition is a strain 1x10 ⁷ to 1x10 ¹¹ cfu / mL, or a composition comprising a culture thereof.
A method of inducing resistance to drought stress in a plant comprising the step of treating the plant or soil with the composition of claim 4.

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