CN116574596A - Activity inspection device and method in microorganism culture process - Google Patents

Activity inspection device and method in microorganism culture process Download PDF

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Publication number
CN116574596A
CN116574596A CN202310865501.5A CN202310865501A CN116574596A CN 116574596 A CN116574596 A CN 116574596A CN 202310865501 A CN202310865501 A CN 202310865501A CN 116574596 A CN116574596 A CN 116574596A
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electrode tube
bearing
seat
guide shaft
activity
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CN116574596B (en
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冯一鸣
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Shandong ZC Yifeng Fertilizer Group Co ltd
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Shandong ZC Yifeng Fertilizer Group Co ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses an activity inspection device and a method thereof in the microorganism cultivation process, and relates to the technical field of microorganism activity inspection. According to the invention, the microbial culture dishes cultured under different environmental conditions are sequentially placed on the bearing trigger mechanism, the comparison analysis display mechanism is driven to rotate, the microbial culture dishes rotate to the microscope of the activity inspection main control mechanism, the pictures are taken by the camera and then transmitted to the comparison analysis display mechanism to be displayed, the taken pictures are watched, the microbial activity is analyzed more intuitively, and the optimal environmental conditions for microbial culture are conveniently determined by comparing the taken pictures.

Description

Activity inspection device and method in microorganism culture process
Technical Field
The invention relates to the technical field of microorganism activity detection, in particular to an activity detection device and method in the microorganism culture process.
Background
Microbiological tests are a method for detecting the type and quantity of microorganisms in samples such as food, water and the environment. It can be used to detect the presence and quantity of microorganisms such as bacteria, fungi, viruses, etc. to determine whether such samples are safe or standard-meeting. Microbiological tests generally include sampling, culturing and identification steps that can help to ensure public health and food safety.
Among the existing patent documents, a filing date is 2019.10.15, and the authority bulletin number is CN 210826175U's a microorganism verifying attachment', and this patent includes the base, the base is hollow structure, be connected with the objective table through drive mechanism in the base, the upper end lateral wall of objective table encircles and is equipped with a plurality of even culture medium, the upper end lateral wall fixedly connected with stand of base, and the upper end lateral wall fixedly connected with mount pad of stand, the lateral wall of stand is connected with the gasbag through extrusion mechanism, the upper end lateral wall of mount pad passes through the upper end lateral wall sealing connection of drain mechanism and gasbag, the one end fixedly connected with microscope of stand is kept away from to the mount pad.
However, when observing the activity states of the microorganism culture under different culture environments through a microscope, the patent only needs to observe one by one, the operation is complicated, and then all the scenes of the microorganism activity states under the microscope cannot be compared one by one, so that the time for determining the environment conditions of the microorganism in the optimal activity state is too long in the culture process.
Disclosure of Invention
The invention aims to solve the problems in the background art and provides an activity testing device and a method thereof in the microorganism culture process.
In order to achieve the above purpose, the invention provides the following technical scheme:
the utility model provides an activity verifying attachment among the microorganism cultivation process, the activity of microorganism in the microorganism culture dish of mainly used cultivateing under the different environmental conditions is inspected, and this activity verifying attachment includes activity inspection main control mechanism, contrast analysis show mechanism and bears trigger mechanism, and contrast analysis show mechanism activity assembly is in the bottom of activity inspection main control mechanism, bears trigger mechanism demountable installation in the upper surface of contrast analysis show mechanism, and the microorganism culture dish is placed in proper order in bearing trigger mechanism.
Through adopting above-mentioned scheme, when using, place microorganism culture dish in proper order in bearing trigger mechanism, control contrast analysis show mechanism rotation through activity inspection main control mechanism, in the rotation process, trigger shooting under the microscope the activity state diagram of microorganism in the microorganism culture dish with this, then show at contrast analysis show mechanism, through watching and shoot the picture, more directly perceived when analyzing microorganism activity, through comparing each picture of shooing, be convenient for confirm microorganism culture's best environmental condition fast.
Further as the preferable scheme, the activity inspection main control mechanism comprises a base, a support and a top seat, wherein the base is fixedly connected with the top seat through the support;
an L-shaped bracket is fixed at one end of the base, a downward driving motor is fixed on the L-shaped bracket, and a driving gear is connected on a rotating shaft of the driving motor in a transmission way;
one side of footstock is provided with down microscope, and microscope department is fixed with annular protection casing directly over, and the top of annular protection casing is provided with the camera, and the camera corresponds directly over being located annular protection casing, and the centre of footstock is provided with control unit.
The control unit comprises a central processor, a first guide shaft is fixed beside the central processor, an electrode tube I is sleeved on the first guide shaft, the first electrode tube is connected with the central processor through a lead, the first guide shaft and the first electrode tube penetrate through the outside of the control unit, a first conductive sheet is fixed on one side of one end of the electrode tube, a guide block is fixed on one side of one end of the electrode tube, far away from the first conductive sheet, and is assembled on the first guide shaft in a sliding manner, a limit head is fixed on one end of the first guide shaft, and a first spring positioned below the guide block is sleeved on the first guide shaft;
the central processing unit is connected with the electrode tube I through a lead;
the central processing unit is respectively connected with the camera and the driving motor in a control way.
Further as the preferred scheme, contrast analysis show mechanism includes the roating seat, and the roating seat passes through bearing and pillar interference fit, and the upper surface of roating seat is provided with many evenly distributed's butt joint, and the side of roating seat is provided with many evenly distributed's display screen, every display screen and the position one-to-one and electric connection of butt joint, and the side of roating seat still is provided with driven fluted disc, and driven fluted disc passes through the chain and meshes with the driving gear mutually.
Further as the preferred scheme, the bearing triggering mechanism comprises a bearing main body, a bearing seat is horizontally arranged above the bearing main body, a second guide shaft is fixed below the bearing seat, the second guide shaft is assembled in the bearing main body in a sliding mode, a second spring positioned below the bearing seat is sleeved on the second guide shaft, a butt joint seat matched with the butt joint is arranged below the bearing main body, and a connecting rib is fixed on one side of the bearing seat;
the bearing triggering mechanism further comprises a conductive triggering tube, the conductive triggering tube is arranged on one side of the bearing main body, a second guide shaft is fixed in the conductive triggering tube, an electrode tube II and an electrode tube III are sleeved on the second guide shaft, the electrode tube II is fixedly assembled, the electrode tube III is movably assembled, the electrode tube II is connected with the butt joint seat through a wire, the electrode tube III is connected with the bearing seat through a connecting rib, the connecting rib is located in a limiting groove, the limiting groove is formed in one side, close to the bearing seat, of the conductive triggering tube, and a conductive sheet II is further fixed at the upper end head of the electrode tube III.
Further, as the preferable scheme, the surface of the first conductive sheet is a sector, so that the sliding contact time of the second conductive sheet and the first conductive sheet can be prolonged, and the shot picture can be ensured to have enough time to complete transmission.
Further, as a preferable scheme, the first guide shaft, the connecting rib and the second guide shaft are all non-electric conductors, such as bakelite and the like, so that good insulativity can be ensured; the first conductive sheet, the second conductive sheet, the first electrode tube, the second electrode tube and the third electrode tube are all conductive bodies, such as aluminum, so that good conductivity can be ensured, and the material is lighter, so that triggering is easy to realize.
Further as the preferred scheme, the top of bearing the main part has still been seted up and has been dodged the mouth, dodges the mouth and be located directly over bearing the seat, avoids hindering the observation of microscope.
An activity test method in a microorganism culture process, the activity test method comprising the steps of:
s1, sequentially placing microorganism culture dishes which are cultured under different environmental conditions on a bearing seat, and limiting by a limiting head;
s2, the bearing seat is pressed downwards under the action of the gravity of the microorganism culture dish, the electrode tube III moves downwards along with the bearing seat, and at the moment, the lower end of the electrode tube III is contacted with the upper end of the electrode tube II;
s3, starting a driving motor through a central processing unit of the control unit, and driving a driven fluted disc to rotate through a driving gear and a chain to realize the rotation of the rotary seat;
s4, when the bearing trigger mechanism rotates to the lower part of the microscope, the conducting strip II at the upper end of the electrode tube III in the bearing trigger mechanism is in sliding contact with the conducting strip I of the electrode tube I, in the process that the conducting strip II is in sliding contact with the conducting strip I, the central processing unit controls the microscope to start and observe microorganisms in the microorganism culture dish, the central processing unit controls the camera to shoot the microorganisms observed by the microscope, at the moment, the electrode tube I, the electrode tube III, the electrode tube II, the butt joint seat and the butt joint form a closed circuit, and then the central processing unit transmits picture signals to the display screen for display through the circuit;
s5, along with the rotation of the rotating seat, all microorganisms in the microorganism culture dishes on the bearing triggering mechanism are transmitted to the display screen to be displayed in the mode, the photographed images are watched, the analysis of the activity of the microorganisms is more visual, and the optimal environment conditions for the microorganism culture are conveniently determined by comparing the photographed images.
Compared with the prior art, the invention has the beneficial effects that:
the microbial culture dishes cultured under different environmental conditions are sequentially placed on the bearing trigger mechanism, the microbial culture dishes are rotated under a microscope of the activity inspection main control mechanism by driving the comparison analysis display mechanism to rotate, photographs are taken by the camera and then transferred to the comparison analysis display mechanism to be displayed, and the photographed photographs are watched to be more visual when the microbial activity is analyzed;
by comparing the pictures, the optimal environmental conditions for microbial cultivation can be determined quickly.
Drawings
FIG. 1 is a schematic diagram of a front view of the present invention;
FIG. 2 is a schematic top view of the present invention;
FIG. 3 is a cross-sectional view taken along line A-A of FIG. 1;
FIG. 4 is an enlarged view of a portion of the structure of FIG. 3;
FIG. 5 is a cross-sectional view taken along line B-B of FIG. 1;
FIG. 6 is an enlarged view of a portion of the structure of FIG. 5;
FIG. 7 is a cross-sectional view of the internal structure of FIG. 1;
FIG. 8 is an enlarged view of a portion of the structure of FIG. 7;
FIG. 9 is an enlarged view of a portion of the structure of FIG. 7;
FIG. 10 is an enlarged view of a portion of the structure of FIG. 7;
FIG. 11 is an enlarged view of a portion of FIG. 9 at C;
FIG. 12 is a partial enlarged view at D in FIG. 9;
fig. 13 is a schematic diagram of the operation of the present invention.
In the figure: 1-a base; a 2-L-shaped bracket; 3-a drive motor; 4-a drive gear; 5-supporting columns; 6-a top seat; 7-microscopy; 8-an annular protective cover; 9-camera; 10-a control unit; 11-guide shaft one; 12-electrode tube I; 13-conducting strip I; 14-a guide block; 15-guiding a first shaft; 16-a positioning head; 17-spring one; 18-a rotating seat; 19-a bearing; 20-butt joint; 21-a display screen; 22-a driven fluted disc; 23-chain; 24-a carrier body; 25-a conductive trigger tube; 26-a bearing seat; 27-a second guide shaft; 28-spring II; 29-an opposite joint seat; 30-connecting ribs; 31-limit grooves; 32-a second guide shaft; 33-electrode tube two; 34-electrode tube three; 35-conducting strip II; 36-avoiding port.
Detailed Description
The following description of the technical solutions in the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, based on the embodiments of the invention, which would be apparent to one of ordinary skill in the art without making any inventive effort are intended to be within the scope of the invention.
Referring to fig. 1-13, the present invention provides a technical solution:
the utility model provides an activity verifying attachment among the microorganism cultivation process, the activity of microorganism in the microorganism culture dish of mainly used cultivateing under the different environmental conditions is inspected, and this activity verifying attachment includes activity inspection main control mechanism, contrast analysis show mechanism and bears trigger mechanism, and contrast analysis show mechanism activity assembly is in the bottom of activity inspection main control mechanism, bears trigger mechanism demountable installation in the upper surface of contrast analysis show mechanism, and the microorganism culture dish is placed in proper order in bearing trigger mechanism.
When the method is applied, the microbial culture dish is sequentially placed in the bearing trigger mechanism, the main control mechanism for activity inspection controls the comparison analysis display mechanism to rotate, in the rotating process, the active state diagram of microorganisms in the microbial culture dish under the microscope is triggered to be shot, then the microbial culture dish is displayed by the comparison analysis display mechanism, the shot pictures are watched, the microbial activity is analyzed more intuitively, and the optimal environmental conditions for microbial culture are conveniently and rapidly determined by comparing the shot pictures.
Specifically, the activity inspection main control mechanism comprises a base 1, a support column 5 and a top seat 6, wherein the base 1 and the top seat 6 are fixedly connected through the support column 5;
an L-shaped bracket 2 is fixed at one end of the base 1, a downward driving motor 3 is fixed on the L-shaped bracket 2, and a driving gear 4 is connected on a rotating shaft of the driving motor 3 in a transmission way;
a downward microscope 7 is arranged beside the top seat 6, an annular protective cover 8 is fixed at the position right above the microscope 7, a camera 9 is arranged above the annular protective cover 8, the camera 9 is correspondingly positioned right above the annular protective cover 8, and a control unit 10 is arranged in the middle of the top seat 6.
The control unit 10 comprises a central processor, a guide shaft I11 is fixed beside the central processor, an electrode tube I12 is sleeved on the guide shaft I11, the electrode tube I12 is connected with the central processor through a lead, the guide shaft I11 and the electrode tube I12 penetrate through the outside of the control unit 10, a conducting sheet I13 is fixed on one side of the end of the electrode tube I12, a guide block 14 is fixed on one side of the end of the electrode tube I12 away from the conducting sheet I13, the guide block 14 is assembled on the guide shaft I15 in a sliding manner, a limit head 16 is fixed on the end of the guide shaft I15, and a spring I17 positioned below the guide block 14 is sleeved on the guide shaft I15;
the central processing unit is connected with the electrode tube I12 through a lead;
the central processing unit is respectively connected with the camera 9 and the driving motor 3 in a control way.
Specifically, the contrast analysis show mechanism includes roating seat 18, and roating seat 18 passes through bearing 19 and pillar 5 interference fit, and the upper surface of roating seat 18 is provided with many evenly distributed's butt joint 20, and the side of roating seat 18 is provided with many evenly distributed's display screen 21, and every display screen 21 and butt joint 20's position one-to-one and electric connection, the side of roating seat 18 still is provided with driven fluted disc 22, and driven fluted disc 22 meshes with driving gear 4 through chain 23.
Specifically, the bearing triggering mechanism comprises a bearing main body 24, a bearing seat 26 is horizontally arranged above the bearing main body 24, a second guide shaft 27 is fixed below the bearing seat 26, the second guide shaft 27 is slidably assembled in the bearing main body 24, a second spring 28 positioned below the bearing seat 26 is sleeved on the second guide shaft 27, a butt seat 29 matched with the butt joint 20 is arranged below the bearing main body 24, and a connecting rib 30 is fixed on one side of the bearing seat 26;
the bearing triggering mechanism further comprises a conductive triggering tube 25, the conductive triggering tube 25 is arranged on one side of the bearing main body 24, a second guide shaft 32 is fixed in the conductive triggering tube 25, an electrode tube 33 and an electrode tube III 34 are sleeved on the second guide shaft 32, the electrode tube II 33 is fixedly assembled, the electrode tube III 34 is movably assembled, the electrode tube II 33 is connected with the butt joint seat 29 through a wire, the electrode tube III 34 is connected with the bearing seat 26 through a connecting rib 30, the connecting rib 30 is positioned in a limiting groove 31, the limiting groove 31 is formed in one side, close to the bearing seat 26, of the conductive triggering tube 25, and a conductive sheet II 35 is further fixed at the upper end of the electrode tube III 34.
In the above, the surface of the first conductive sheet 13 is a sector, so that the sliding contact time between the second conductive sheet 35 and the first conductive sheet 13 can be prolonged, and the photographed picture can be ensured to have enough time to complete transmission.
Among the above, the guide shaft one 11, the guide shaft one 15, the connection rib 30, and the guide shaft two 32 are all nonconductive bodies, such as bakelite, etc., so that good insulation can be ensured; the first conductive sheet 13, the second conductive sheet 35, the first electrode tube 12, the second electrode tube 33 and the third electrode tube 34 are all conductive bodies, such as aluminum, so that good conductivity can be ensured, and the material is light, so that triggering is easy to realize.
In the above description, the avoidance port 36 is further formed above the bearing main body 24, and the avoidance port 36 is located directly above the bearing seat 26, so as to avoid obstructing the observation of the microscope 7.
An activity test method in a microorganism culture process, the activity test method comprising the steps of:
s1, sequentially placing the microorganism culture dishes cultured under different environmental conditions on a bearing seat 26, and limiting by a limiting head 16;
s2, the bearing seat 26 is pressed downwards under the action of the gravity of the microorganism culture dish, the electrode tube III 34 moves downwards along with the bearing seat 26, and at the moment, the lower end of the electrode tube III 34 is contacted with the upper end of the electrode tube II 33;
s3, starting a driving motor 3 through a central processing unit of the control unit 10, and driving a driven fluted disc 22 to rotate through a driving gear 4 and a chain 23 to realize the rotation of the rotary seat 18;
s4, when the bearing trigger mechanism rotates to the position below the microscope 7, the second conductive sheet 35 at the upper end of the third electrode tube 34 in the bearing trigger mechanism is in sliding contact with the first conductive sheet 13 of the first electrode tube 12, in the process that the second conductive sheet 35 is in sliding contact with the first conductive sheet 13, the CPU controls the microscope 7 to start and observe microorganisms in a microorganism culture dish, the CPU further controls the camera 9 to photograph the microorganisms observed by the microscope 7, at the moment, the first electrode tube 12, the third electrode tube 34, the second electrode tube 33, the butt joint seat 29 and the butt joint 20 form a closed circuit, and then the CPU transmits picture signals to the display screen 21 for display through the circuit;
s5, along with the rotation of the rotating seat 18, all microorganisms in the microorganism culture dishes on the bearing triggering mechanism transmit the shot pictures to the display screen 21 for display in the mode, the shot pictures are watched, the analysis of the activity of the microorganisms is more visual, and the optimal environment conditions for the microorganism culture are conveniently determined by comparing the shot pictures.
In the above, when the support base 26 is not placed with the microbial culture dish, the third electrode tube 34 and the second electrode tube 33 are not contacted with each other, and even if the support trigger mechanism rotates under the microscope 7, the second conductive sheet 35 at the upper end of the third electrode tube 34 is not contacted with the first conductive sheet 13 of the first electrode tube 12.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims (9)

1. An activity testing device in the microorganism culture process is mainly used for testing the activity of microorganisms in a microorganism culture dish cultured under different environmental conditions, and is characterized in that: the activity inspection device comprises an activity inspection main control mechanism, a comparison analysis display mechanism and a bearing trigger mechanism, wherein the comparison analysis display mechanism is movably assembled at the bottom of the activity inspection main control mechanism, the bearing trigger mechanism is detachably arranged on the upper surface of the comparison analysis display mechanism, and the microbial culture dishes are sequentially placed in the bearing trigger mechanism.
2. An activity test device in a microorganism culture process according to claim 1, wherein: the activity inspection main control mechanism comprises a base (1), a support (5) and a top seat (6), wherein the base (1) and the top seat (6) are fixedly connected through the support (5);
an L-shaped bracket (2) is fixed at one end of the base (1), a downward driving motor (3) is fixed on the L-shaped bracket (2), and a driving gear (4) is connected on a rotating shaft of the driving motor (3) in a transmission manner;
the microscope is characterized in that a downward microscope (7) is arranged beside the top seat (6), an annular protection cover (8) is fixed at the position right above the microscope (7), a camera (9) is arranged above the annular protection cover (8), the camera (9) is correspondingly positioned right above the annular protection cover (8), and a control unit (10) is arranged in the middle of the top seat (6).
3. An activity test device in a microorganism culture process according to claim 2, wherein: the control unit (10) comprises a central processor, a guide shaft I (11) is fixed beside the central processor, an electrode tube I (12) is sleeved on the guide shaft I (11), the electrode tube I (12) is connected with the central processor through a lead, the guide shaft I (11) and the electrode tube I (12) penetrate through the outside of the control unit (10), a conducting strip I (13) is fixed on one side of the end of the electrode tube I (12), a guide block (14) is fixed on one side of the end of the electrode tube I (12) away from the conducting strip I (13), the guide block (14) is slidingly assembled on the guide shaft I (15), a limit head (16) is fixed on the end of the guide shaft I (15), and a spring I (17) positioned below the guide block (14) is sleeved on the guide shaft I (15);
the central processing unit is connected with the electrode tube I (12) through a lead;
the central processing unit is respectively connected with the camera (9) and the driving motor (3) in a control way.
4. A device for testing the activity of a microorganism in a cultivation process according to claim 3, wherein: the contrast analysis show mechanism includes roating seat (18), roating seat (18) are through bearing (19) and pillar (5) interference fit, the upper surface of roating seat (18) is provided with many evenly distributed's butt joint (20), the side of roating seat (18) is provided with many evenly distributed's display screen (21), every display screen (21) and the position one-to-one and electric connection of butt joint (20), the side of roating seat (18) still is provided with driven fluted disc (22), driven fluted disc (22) are through chain (23) and driving gear (4) meshing.
5. The activity test device in a microorganism culture process according to claim 4, wherein: the bearing triggering mechanism comprises a bearing main body (24), a bearing seat (26) is horizontally arranged above the bearing main body (24), a second guide shaft (27) is fixed below the bearing seat (26), the second guide shaft (27) is slidably assembled in the bearing main body (24), a second spring (28) positioned below the bearing seat (26) is sleeved on the second guide shaft (27), a butt joint seat (29) matched with the butt joint head (20) is arranged below the bearing main body (24), and a connecting rib (30) is fixed on one side of the bearing seat (26);
the bearing triggering mechanism further comprises a conductive triggering tube (25), the conductive triggering tube (25) is arranged on one side of the bearing main body (24), a second guide shaft (32) is fixed in the conductive triggering tube (25), a second electrode tube (33) and a third electrode tube (34) are sleeved on the second guide shaft (32), the second electrode tube (33) is fixedly assembled, the third electrode tube (34) is movably assembled, the second electrode tube (33) is connected with the butt joint seat (29) through a wire, the third electrode tube (34) is connected with the bearing seat (26) through a connecting rib (30), the connecting rib (30) is located in a limiting groove (31), the limiting groove (31) is formed in one side, close to the bearing seat (26), of the conductive triggering tube (25), and a second conductive sheet (35) is further fixed at the upper end of the third electrode tube (34).
6. An activity test device in a microorganism culture process according to any one of claims 3 to 5, wherein: the surface of the first conductive sheet (13) is a sector.
7. An activity test device in a microorganism culture process according to claim 5, wherein: the first guide shaft (11), the first guide shaft (15), the connecting rib (30) and the second guide shaft (32) are all non-conductors, and the first conductive sheet (13), the second conductive sheet (35), the first electrode tube (12), the second electrode tube (33) and the third electrode tube (34) are all conductors.
8. An activity test device in a microorganism culture process according to claim 5, wherein: an avoidance port (36) is further formed in the upper portion of the bearing main body (24), and the avoidance port (36) is located right above the bearing seat (26).
9. The method of claim 5, wherein the step of using the activity test device in the microorganism culture process is characterized by: the using method is as follows:
s1, sequentially placing microorganism culture dishes cultured under different environmental conditions on a bearing seat (26) and limiting the microorganism culture dishes through a limiting head (16);
s2, the bearing seat (26) is pressed downwards under the action of the gravity of the microorganism culture dish, the electrode tube III (34) moves downwards along with the bearing seat (26), and at the moment, the lower end of the electrode tube III (34) is contacted with the upper end of the electrode tube II (33);
s3, starting a driving motor (3) through a central processing unit of the control unit (10), and driving a driven fluted disc (22) to rotate through a driving gear (4) by a chain (23) so as to realize the rotation of a rotating seat (18);
s4, when the bearing trigger mechanism rotates to the position below the microscope (7), a conducting strip II (35) at the upper end of an electrode tube III (34) in the bearing trigger mechanism is in sliding contact with a conducting strip I (13) of an electrode tube I (12), in the process that the conducting strip II (35) is in sliding contact along the conducting strip I (13), the central processing unit controls the microscope (7) to start and observe microorganisms in a microorganism culture dish, the central processing unit controls the camera (9) to photograph the microorganisms observed by the microscope (7), at the moment, the electrode tube I (12), the electrode tube III (34), the electrode tube II (33), the butt joint seat (29) and the butt joint (20) form a closed circuit, and then the central processing unit transmits picture signals to the display screen (21) through the circuit for displaying;
s5, along with the rotation of the rotating seat (18), all microorganisms in the microorganism culture dishes on the bearing triggering mechanism transmit the photographed pictures to the display screen (21) for display in the mode, the photographed pictures are more visual when the microbial activity is analyzed, and the optimal environmental conditions for microbial cultivation are conveniently determined by comparing the photographed pictures.
CN202310865501.5A 2023-07-14 2023-07-14 Activity inspection device and method in microorganism culture process Active CN116574596B (en)

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CN202310865501.5A CN116574596B (en) 2023-07-14 2023-07-14 Activity inspection device and method in microorganism culture process

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