CN1540434A - Method and device for observing and analyzing image of microbe quantificationally - Google Patents
Method and device for observing and analyzing image of microbe quantificationally Download PDFInfo
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- CN1540434A CN1540434A CNA2003101083840A CN200310108384A CN1540434A CN 1540434 A CN1540434 A CN 1540434A CN A2003101083840 A CNA2003101083840 A CN A2003101083840A CN 200310108384 A CN200310108384 A CN 200310108384A CN 1540434 A CN1540434 A CN 1540434A
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- aperture
- tooth bar
- guide rail
- slide block
- bar guide
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- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The device includes mechanism for adjusting aperture, mechanism for adjusting focal length, composite light source of transmission and direct projection, high-resolution wide angle CCD camera. Mechanism for adjusting aperture is assorted with mechanism for adjusting focal length in odder to fit images of different sizes and fields in quantitative observation and analysis. Cold light sources in upper and lower provide culture dish even transmitted light and direct projection light. Through wide-angle camera, CCD collects images, and transfers the images to computer for automatic statistics and analysis.
Description
Technical field
The present invention relates to electromechanics and electronic information field, relate in particular to a kind of microorganism image quantitative observation and analytical approach and device thereof.
Background technology
In fields such as biology, medical science, food, pharmacy and chemical industry, all need form, quantity to observe and statistical study to microorganism.Traditional method of with the naked eye observing and adding up is owing to small bacterium colony being difficult to observe clear and influence statistical accuracy easily.The method that adopts computer picture to observe and analyze can effectively improve the sharpness that microorganism is observed, and helps the statistical study of form, size.But owing to need the situation difference of the microorganism of observation, differing in size of observed double dish adopts fixing image viewing can reduce the resolution of image, influences the accuracy of observing effect and statistical study.Thereby change the visual field if change focal length, can destroy the scale-up factor of prior regulation in the computing machine again, cause statistical study inaccurate.
Summary of the invention
The purpose of this invention is to provide a kind of microorganism image quantitative observation and analytical approach and device thereof.
Microorganism image quantitative observation and analytical equipment: have aperture governor motion, focus adjustment mechanism, transmission direct projection composite light source, the vast CCD camera of high image resolution on the base.Aperture governor motion: have large aperture or large aperture+middle aperture or large aperture+middle aperture+little aperture on the interior round seam of chassis left side platform successively; Transmission direct projection composite light source: annular lamp tube has been fixed in the bottom at slide block, for double dish provides the direct projection light source, the CCD camera is fixed on the top of slide block, the centre of annular lamp tube, below the inside of base, aperture, fixed light guide plate, two cold cloudy fluorescent tubes have been fixed in the both sides of light guide plate, thereby provide enough strong and uniform transmitted light for double dish, annular lamp tube, two cold cloudy fluorescent tubes are controlled by inverter; The vast CCD camera of high image resolution: on slide block, the centre of annular lamp tube is provided with CCD camera, macro wide lens.
Microorganism image viewing and quantitative analysis method: adopt claim 1 and 2 described aperture governor motion and focus adjustment mechanisms, when different apertures is cooperated with focal length, the image that observes and pass to computing machine from CCD all reaches full display screen demonstration, also is that display image all reaches maximum; Again every kind of aperture is demarcated with image and material object under focal length cooperates, and deposit this calibration coefficient in computing machine, during use, according to the size that is observed thing, select cooperating of certain aperture and focal length, in computing machine, select corresponding calibration coefficient again, can be observed thing and observe changing, and carry out quantitative test.
Advantage of the present invention:
1) staking-out work bothers very much, needs specialized equipment, and general user can't carry out.Because double dish kind commonly used is limited, adopt the device of the present invention's design, adopt three kinds of different aperture combinations as long as change, adjusting slider is in three differing heights simultaneously, just can adapt to the double dish of different size.This method is simple in structure, and is very easy to use, rapid;
2) first half of aperture is used to lay the double dish or little No. one aperture of three kinds of different sizes, thereby keeps with one heart; Circle is used to limit the transmission area of bottom light source in the Lower Half of aperture, prevents the reflective interference of double dish marginal portion, thereby also has the effect that improves picture quality;
3) transmitted light source of design adopts two cold cloudy fluorescent tubes, has guaranteed the sufficient intensity and the homogeneity of light source.The direct projection of the transmission up and down Composite Double light source of design, more traditional single light source more improves picture quality, thereby guarantees correct observation and analysis to microbe colony;
4) can unite the microorganism image viewing analytical equipment of adjusting to aperture and focal length easily, several grades of fixing in advance in coupled computer scale-up factors are selected, and can carry out image viewing and quantitative statistical analysis under the optimum resolution to the double dish of different size.Do not need to demarcate again because of the change focal length or the visual field; Improve the accuracy of microorganism observation analysis, reduce labor intensity.
Description of drawings
Accompanying drawing is based on the microorganism observation analysis system architecture synoptic diagram of image recognition; Among the figure: base 1, large aperture 2a, middle aperture 2b, little aperture 2c, cold cloudy fluorescent tube 3, light guide plate 4, inverter 5, large size double dish 6a, medium size double dish 6b, small size double dish 6c, tooth bar guide rail 7, slide block 8, steel ball 9, spring 10, screw 11, regulating shaft 12, garden gear 13, annular lamp tube 14, CCD camera 15, macro wide lens 16.
Embodiment
Have aperture governor motion, focus adjustment mechanism, transmission direct projection composite light source, the vast CCD camera of high image resolution on the base 1 based on the microorganism observation analysis system of image recognition.Aperture governor motion: have among large aperture 2a or the large aperture 2a+ the little aperture 2c of aperture 2b+ among aperture 2b or the large aperture 2a+ on the interior round seam of base 1 left side platform successively; Transmission direct projection composite light source: fixed annular lamp tube 14 in the bottom of slide block 8, for double dish provides the direct projection light source, the CCD camera is fixed on the top of slide block 8, the centre of annular lamp tube 14, below the inside of base 1, aperture 2a, be provided with light guide plate 4, the both sides of light guide plate 4 are provided with two cold cloudy fluorescent tubes 3, thereby provide enough strong and uniform transmitted light for double dish, 14, two cold cloudy fluorescent tubes 3 of annular lamp tube are by inverter 5 controls; The vast CCD camera of high image resolution: on slide block, the centre of annular lamp tube is provided with CCD camera 15, macro wide lens 16.
Focus adjustment mechanism: base 1 right side is provided with tooth bar guide rail 7, is provided with slide block 8 on tooth bar guide rail 7; Tooth bar is processed in the left side of tooth bar guide rail 7, and regulating shaft 12 has been installed on the slide block 8, and regulating shaft 12 is provided with Knucle-gear 13, and rotation regulating shaft 12 drives Knucle-gear 13 and rotates, and by the engagement of knuckle-tooth wheel and rack, drive slide block 8 prolongs tooth bar guide rail 7 and moves up and down; The right flank of tooth bar guide rail 7 is drilled with three bellmouths (going up taper hole, middle taper hole, lower cone hole) from top to bottom, the right side of slide block 8 is equipped with steel ball 9 and spring 10, when steel ball runs into the bellmouth of tooth bar guide rail 7 right flanks, spring (10) can imbed by compressing steel ball (9), thereby slide block (8) is remained on a certain fixing height; Circle cooperates with the cylindrical of middle aperture 2b in the last seam of large aperture 2a in the aperture governor motion, is cooperated with the cylindrical of little aperture 2c by circle in the last seam of aperture 2b, by that analogy.。
As shown in drawings, part 2a～2c forms the aperture governor motion, and 7～13 form focus adjustment mechanism, and 3～5 and 14 form transmission direct projection composite light source, and 15～16 form the vast CCD camera of high image resolution.The outer warp of large aperture 2a cooperates with the interior round seam of base 1, thereby keeps same center with camera 15; Interior round seam on the large aperture 2a can the number of amplification double dish 6a, aperture 2b in also can installing; Interior round seam on the middle aperture 2b can be put medium size double dish 6b, and little aperture 2c also can be installed; Interior round seam on the little aperture 2c can be put small size double dish 6c.The left side of straight-tooth guide rail 7 is the tooth bars that are processed into, regulating shaft 12 has been installed on the slide block 8, has been fixed Knucle-gear 13 on the regulating shaft 12, rotation regulating shaft 12 can drive Knucle-gear 13 and rotate, by the engagement of knuckle-tooth wheel and rack, can drive slide block 8 and prolong straight-tooth guide rail 7 and move up and down.Be drilled with three bellmouths on the right flank of straight-tooth guide rail 7, the right side of slide block 8 is equipped with steel ball 9 and spring 10, when steel ball runs into the bellmouth of straight-tooth guide rail 7 right flanks, can imbed, thereby slide block 8 is remained on a certain fixing height.Two cold cloudy fluorescent tubes are installed on the both sides of light guide plate 4 respectively, and even transmitted light source is provided; Cold cloudy ring fluorescent tube 14 has been installed in the slide block lower end, for double dish provides direct light.The front end of CCD camera 15 has been installed macro wide lens 16, and the visual field of CCD camera 15 is enlarged.
According to varying in size of double dish, select to adopt three kinds of different aperture combination 2a, 2a+2b or 2a+2b+2c, adjusting slider 8 is arranged in the pairing position of tooth bar guide rail 7 top-down three bellmouths (going up taper hole, taper hole, lower cone hole) simultaneously, forms three kinds of different image viewing patterns; In computer software, select three kinds of predefined different scale-up factors simultaneously, can form image viewing and quantitative test under the ultimate resolution the double dish of three kinds of different sizes.Transmission direct projection composite light source adopt the negative electrode cold light source for double dish provide from bottom to top transmitted light and direct light from top to bottom, form strong and uniform bias light.High image resolution CCD cooperates with macro wide lens, the high-definition camera structure that formation can closely be made a video recording.The double dish image is taken in computing machine, just can carry out image viewing and quantitative statistical analysis the microbe colony on this double dish.
Claims (5)
1. microorganism image quantitative observation and analytical equipment is characterized in that aperture governor motion, focus adjustment mechanism, transmission direct projection composite light source, the vast CCD camera of high image resolution that it has base (1) and is provided with.Aperture governor motion: be provided with large aperture (2a) or large aperture (2a)+middle aperture (2b) or large aperture (2a)+middle aperture (2b)+little aperture (2c) successively on the interior round seam of base (1) left side platform; Transmission direct projection composite light source: fixed annular lamp tube (14) in the bottom of slide block (8), for double dish provides the direct projection light source, the CCD camera is fixed on the top of slide block (8), the centre of annular lamp tube (14), below the inside of base 1, aperture (2a), be provided with light guide plate (4), the both sides of light guide plate (4) are provided with two cold cloudy fluorescent tubes (3), thereby provide enough strong and uniform transmitted light for double dish, annular lamp tube (14), two cold cloudy fluorescent tubes (3) are controlled by inverter (5); The vast CCD camera of high image resolution: on slide block, the centre of annular lamp tube is provided with CCD camera (15), macro wide lens (16).
2. a kind of microorganism image quantitative observation according to claim 1 and analytical equipment, it is characterized in that said focus adjustment mechanism: base (1) right side is provided with tooth bar guide rail (7), is provided with slide block (8) on tooth bar guide rail (7); Tooth bar is processed in the left side of tooth bar guide rail (7), regulating shaft (12) has been installed on the slide block (8), regulating shaft (12) is provided with Knucle-gear (13), rotation regulating shaft (12) drives Knucle-gear (13) and rotates, by the engagement of knuckle-tooth wheel and rack, drive slide block (8) prolongs tooth bar guide rail (7) and moves up and down; The right flank of tooth bar guide rail (7) is drilled with three bellmouths (going up taper hole, middle taper hole, lower cone hole) from top to bottom, the right side of slide block (8) is equipped with steel ball (9) and spring (10), when steel ball runs into the bellmouth of tooth bar guide rail (7) right flank, spring (10) can imbed by compressing steel ball (9), thereby slide block (8) is remained on a certain fixing height;
3. a kind of microorganism image quantitative observation according to claim 1 and analytical equipment, the interior circle of last seam that it is characterized in that the aperture (2a) in the said aperture governor motion cooperates with the cylindrical of aperture (2b), and circle cooperates with the cylindrical of aperture (2c) in the last seam of aperture (2b).
4. microorganism image viewing and quantitative analysis method, it is characterized in that adopting claim 1 and 2 described aperture governor motion and focus adjustment mechanisms, when different apertures is cooperated with focal length, the image that observes and pass to computing machine from CCD all reaches full display screen demonstration, also is that display image all reaches maximum; Again every kind of aperture is demarcated with image and material object under focal length cooperates, and deposit this calibration coefficient in computing machine, during use, according to the size that is observed thing, select cooperating of certain aperture and focal length, in computing machine, select corresponding calibration coefficient again, can be observed thing and observe changing, and carry out quantitative test.
5. a kind of microorganism image quantitative observation according to claim 4 and analytical approach, it is characterized in that: said employing claim 1 and the aperture governor motion and during focus adjustment mechanism, large aperture (2a) is used with the last taper hole of tooth bar guide rail (7) right flank; Large aperture (2a)+middle aperture (2b) is used with the middle taper hole of tooth bar guide rail (7) right flank; Large aperture (2a)+middle aperture (2b)+little aperture (2c) is used with the lower cone hole of tooth bar guide rail (7) right flank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2003101083840A CN1540434A (en) | 2003-10-30 | 2003-10-30 | Method and device for observing and analyzing image of microbe quantificationally |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2003101083840A CN1540434A (en) | 2003-10-30 | 2003-10-30 | Method and device for observing and analyzing image of microbe quantificationally |
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| CN1540434A true CN1540434A (en) | 2004-10-27 |
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| CNA2003101083840A Pending CN1540434A (en) | 2003-10-30 | 2003-10-30 | Method and device for observing and analyzing image of microbe quantificationally |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103517676A (en) * | 2010-09-27 | 2014-01-15 | 奥克索金股份有限公司 | Apparatus, method, and system for automated imaging and evaluation of embryos, oocytes, and stem cells |
| CN105062878A (en) * | 2015-08-26 | 2015-11-18 | 上海理工大学 | Mixed illuminating system applied to automatic bacterial colony selecting instrument |
| CN106405984A (en) * | 2015-07-30 | 2017-02-15 | 富士施乐株式会社 | Unit for photographing and photographing system |
| CN108810364A (en) * | 2018-04-28 | 2018-11-13 | 刘红兵 | The visual extraction apparatus of trace material evidence |
| CN109030225A (en) * | 2018-06-29 | 2018-12-18 | 中国电建集团中南勘测设计研究院有限公司 | A kind of equipment for observing Rockmass fractures in the test of hydraulic fracturing crustal stress in real time |
| CN116574596A (en) * | 2023-07-14 | 2023-08-11 | 山东中创亿丰肥料集团有限公司 | Activity inspection device and method in microorganism culture process |
-
2003
- 2003-10-30 CN CNA2003101083840A patent/CN1540434A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103517676A (en) * | 2010-09-27 | 2014-01-15 | 奥克索金股份有限公司 | Apparatus, method, and system for automated imaging and evaluation of embryos, oocytes, and stem cells |
| CN106405984A (en) * | 2015-07-30 | 2017-02-15 | 富士施乐株式会社 | Unit for photographing and photographing system |
| CN105062878A (en) * | 2015-08-26 | 2015-11-18 | 上海理工大学 | Mixed illuminating system applied to automatic bacterial colony selecting instrument |
| CN108810364A (en) * | 2018-04-28 | 2018-11-13 | 刘红兵 | The visual extraction apparatus of trace material evidence |
| CN109030225A (en) * | 2018-06-29 | 2018-12-18 | 中国电建集团中南勘测设计研究院有限公司 | A kind of equipment for observing Rockmass fractures in the test of hydraulic fracturing crustal stress in real time |
| CN116574596A (en) * | 2023-07-14 | 2023-08-11 | 山东中创亿丰肥料集团有限公司 | Activity inspection device and method in microorganism culture process |
| CN116574596B (en) * | 2023-07-14 | 2023-09-12 | 山东中创亿丰肥料集团有限公司 | Activity inspection device and method in microorganism culture process |
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