CN116508914A - Composite additive and method for improving utilization rate of high-starch feed by micropterus salmoides - Google Patents
Composite additive and method for improving utilization rate of high-starch feed by micropterus salmoides Download PDFInfo
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- CN116508914A CN116508914A CN202310726884.8A CN202310726884A CN116508914A CN 116508914 A CN116508914 A CN 116508914A CN 202310726884 A CN202310726884 A CN 202310726884A CN 116508914 A CN116508914 A CN 116508914A
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- feed
- micropterus salmoides
- liver
- composite additive
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- 241001125889 Micropterus salmoides Species 0.000 title claims abstract description 39
- 239000000654 additive Substances 0.000 title claims abstract description 36
- 230000000996 additive effect Effects 0.000 title claims abstract description 35
- 239000002131 composite material Substances 0.000 title claims abstract description 21
- 229920002472 Starch Polymers 0.000 title claims abstract description 17
- 239000008107 starch Substances 0.000 title claims abstract description 17
- 238000000034 method Methods 0.000 title claims abstract description 6
- AGBQKNBQESQNJD-UHFFFAOYSA-N lipoic acid Chemical compound OC(=O)CCCCC1CCSS1 AGBQKNBQESQNJD-UHFFFAOYSA-N 0.000 claims abstract description 18
- 150000001875 compounds Chemical class 0.000 claims abstract description 14
- CWVRJTMFETXNAD-FWCWNIRPSA-N 3-O-Caffeoylquinic acid Natural products O[C@H]1[C@@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-FWCWNIRPSA-N 0.000 claims abstract description 9
- PZIRUHCJZBGLDY-UHFFFAOYSA-N Caffeoylquinic acid Natural products CC(CCC(=O)C(C)C1C(=O)CC2C3CC(O)C4CC(O)CCC4(C)C3CCC12C)C(=O)O PZIRUHCJZBGLDY-UHFFFAOYSA-N 0.000 claims abstract description 9
- CWVRJTMFETXNAD-KLZCAUPSSA-N Neochlorogenin-saeure Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O CWVRJTMFETXNAD-KLZCAUPSSA-N 0.000 claims abstract description 9
- LCQLHJZYVOQKHU-VKHMYHEASA-N carglumic acid Chemical compound NC(=O)N[C@H](C(O)=O)CCC(O)=O LCQLHJZYVOQKHU-VKHMYHEASA-N 0.000 claims abstract description 9
- CWVRJTMFETXNAD-JUHZACGLSA-N chlorogenic acid Chemical compound O[C@@H]1[C@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-JUHZACGLSA-N 0.000 claims abstract description 9
- 229940074393 chlorogenic acid Drugs 0.000 claims abstract description 9
- FFQSDFBBSXGVKF-KHSQJDLVSA-N chlorogenic acid Natural products O[C@@H]1C[C@](O)(C[C@@H](CC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O FFQSDFBBSXGVKF-KHSQJDLVSA-N 0.000 claims abstract description 9
- 235000001368 chlorogenic acid Nutrition 0.000 claims abstract description 9
- BMRSEYFENKXDIS-KLZCAUPSSA-N cis-3-O-p-coumaroylquinic acid Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)cc2)[C@@H]1O)C(=O)O BMRSEYFENKXDIS-KLZCAUPSSA-N 0.000 claims abstract description 9
- 235000019136 lipoic acid Nutrition 0.000 claims abstract description 9
- 150000005856 steroid saponins Chemical class 0.000 claims abstract description 9
- 229960002663 thioctic acid Drugs 0.000 claims abstract description 9
- 238000002360 preparation method Methods 0.000 claims description 3
- 235000000346 sugar Nutrition 0.000 claims description 3
- AICOOMRHRUFYCM-ZRRPKQBOSA-N oxazine, 1 Chemical group C([C@@H]1[C@H](C(C[C@]2(C)[C@@H]([C@H](C)N(C)C)[C@H](O)C[C@]21C)=O)CC1=CC2)C[C@H]1[C@@]1(C)[C@H]2N=C(C(C)C)OC1 AICOOMRHRUFYCM-ZRRPKQBOSA-N 0.000 claims description 2
- 210000004185 liver Anatomy 0.000 abstract description 30
- 229920002527 Glycogen Polymers 0.000 abstract description 9
- 229940096919 glycogen Drugs 0.000 abstract description 9
- 238000009825 accumulation Methods 0.000 abstract description 6
- 239000003674 animal food additive Substances 0.000 abstract description 5
- 230000004083 survival effect Effects 0.000 abstract description 5
- 230000001105 regulatory effect Effects 0.000 abstract description 4
- 239000002994 raw material Substances 0.000 abstract description 3
- HVCOBJNICQPDBP-UHFFFAOYSA-N 3-[3-[3,5-dihydroxy-6-methyl-4-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyoxan-2-yl]oxydecanoyloxy]decanoic acid;hydrate Chemical compound O.OC1C(OC(CC(=O)OC(CCCCCCC)CC(O)=O)CCCCCCC)OC(C)C(O)C1OC1C(O)C(O)C(O)C(C)O1 HVCOBJNICQPDBP-UHFFFAOYSA-N 0.000 abstract description 2
- 241000723298 Dicentrarchus labrax Species 0.000 abstract description 2
- 229930186217 Glycolipid Natural products 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 230000004060 metabolic process Effects 0.000 abstract description 2
- 230000003647 oxidation Effects 0.000 abstract description 2
- 238000007254 oxidation reaction Methods 0.000 abstract description 2
- 235000019698 starch Nutrition 0.000 description 6
- 241000251468 Actinopterygii Species 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 102000006587 Glutathione peroxidase Human genes 0.000 description 2
- 108700016172 Glutathione peroxidases Proteins 0.000 description 2
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 description 2
- 241000269800 Percidae Species 0.000 description 2
- 102000019197 Superoxide Dismutase Human genes 0.000 description 2
- 108010012715 Superoxide dismutase Proteins 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 229940118019 malondialdehyde Drugs 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 208000004930 Fatty Liver Diseases 0.000 description 1
- 206010019708 Hepatic steatosis Diseases 0.000 description 1
- NPGIHFRTRXVWOY-UHFFFAOYSA-N Oil red O Chemical compound Cc1ccc(C)c(c1)N=Nc1cc(C)c(cc1C)N=Nc1c(O)ccc2ccccc12 NPGIHFRTRXVWOY-UHFFFAOYSA-N 0.000 description 1
- 241000269801 Perca Species 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 208000019425 cirrhosis of liver Diseases 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 235000021373 diet component Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 208000010706 fatty liver disease Diseases 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000004898 kneading Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000001007 puffing effect Effects 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 231100000240 steatosis hepatitis Toxicity 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 230000004584 weight gain Effects 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/121—Heterocyclic compounds containing oxygen or sulfur as hetero atom
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Insects & Arthropods (AREA)
- Marine Sciences & Fisheries (AREA)
- Birds (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Fodder In General (AREA)
Abstract
The invention belongs to the technical field of feed additives, and particularly relates to a composite additive and a method for improving the utilization rate of a high-starch feed by micropterus salmoides. The invention provides a feed additive for regulating liver health of micropterus salmoides, which comprises N-carbamylglutamic acid, alpha-lipoic acid, steroid saponin and chlorogenic acid. The composite additive is added into the feed according to the proportion when the raw materials of the high-starch feed are mixed, and the feed is directly fed in the young sea bass stage. The compound additive can regulate liver glycolipid metabolism during the growth and development of the micropterus salmoides, and avoid the liver of the micropterus salmoides in a high-pressure state of excessive accumulation of glycogen and fat during the development. Thereby regulating liver health of the micropterus salmoides and improving survival and growth of the micropterus salmoides. The invention is beneficial to reducing the accumulation of liver glycogen and liver fat of the micropterus salmoides after being added into high-starch feed, and improving the growth performance and the liver oxidation resistance.
Description
Technical Field
The invention belongs to the technical field of feed additives, and particularly relates to a composite additive and a method for improving the utilization rate of a high-starch feed by micropterus salmoides.
Background
The micropterus salmoides (academic name: micropterus salmoides) is a fish belonging to genus Laterus of family Laterus, and is also called California Perca. According to the year's book of the year's of the year of the Chinese fishery of 2022, the culture yield of the micropterus salmoides in China reaches 70.21 ten thousand tons, and the culture yield continues to increase with the increment of about 10 ten thousand tons every year. At present, the micropterus salmoides have solved the problem from feeding ice fresh fish to compound feed.
However, the carbohydrate is not well utilized by the micropterus salmoides, so that the starch content of the micropterus salmoides compound feed is only 12% at maximum. Starch is not only the cheapest energy source in the practical diet components, but also the adhesive which is necessary to be used in the aquatic feed, so that the loss of the feed in water can be reduced, the efficiency is improved, and the puffing degree of the compound feed is greatly improved. The weever is a natural diabetic patient with very low tolerance to carbohydrates. Excessive starch can easily cause the liver to be incapable of timely digestion and transportation, and can accumulate in the liver in the form of fat and glycogen, so that fatty liver, sugar liver and even liver cirrhosis can easily occur. Excessive accumulation of fat and glycogen in liver can make liver in high pressure state for a long period of time, and can seriously affect liver health, so that liver antioxidant level can reduce infection of various diseases. Seriously affecting the growth and survival rate of the micropterus salmoides. The intensive cultivation is greatly limited.
Disclosure of Invention
The invention uses a compound additive to regulate and control the liver health development of the micropterus salmoides under high starch feeding. The invention improves the survival rate and growth of the starch feed fed to the micropterus salmoides, reduces the accumulation of liver glycogen and fat, and improves the antioxidant capacity so as to improve the liver health of the micropterus salmoides.
The invention provides a compound additive for improving the utilization rate of high-starch feed by micropterus salmoides, which comprises the following components: n-carbamylglutamic acid, alpha-lipoic acid, steroid saponin and chlorogenic acid.
Preferably, the composite additive comprises, in parts by weight: 2-6 parts of N-carbamylglutamic acid, 3-7 parts of alpha-lipoic acid, 1-3 parts of steroid saponin and 1-5 parts of chlorogenic acid.
Preferably, the composite additive comprises, in parts by weight: 2 parts of N-carbamylglutamic acid, 4 parts of alpha-lipoic acid, 1 part of steroid saponin and 3 parts of chlorogenic acid.
It is another object of the present invention to provide a process for preparing the above composite additive comprising mixing the components.
Another object of the present invention is to provide the use of the above-described composite additive in the preparation of feed for micropterus salmoides.
Another object of the present invention is to provide a feed for micropterus salmoides containing the above compound additive.
Preferably, the addition amount of the compound additive is 1.0-5.5 g/kg of the feed for the micropterus salmoides.
Preferably, the addition amount of the compound additive is 2.0g/kg of the feed for the micropterus salmoides.
Preferably, the feed for the micropterus salmoides further comprises high sugar feed.
The invention provides a feed additive for regulating liver health of micropterus salmoides, which comprises N-carbamylglutamic acid, alpha-lipoic acid, steroid saponin and chlorogenic acid. The composite additive is added into the feed according to the proportion when the raw materials of the high-starch feed are mixed, and the feed is directly fed in the young sea bass stage. The compound additive can regulate liver glycolipid metabolism during the growth and development of the micropterus salmoides, and avoid the liver of the micropterus salmoides in a high-pressure state of excessive accumulation of glycogen and fat during the development. Thereby regulating liver health of the micropterus salmoides and improving survival and growth of the micropterus salmoides. The invention is beneficial to reducing the accumulation of liver glycogen and liver fat of the micropterus salmoides after being added into high-starch feed, and improving the growth performance and the liver oxidation resistance.
Drawings
FIG. 1 is a graph of serum triglyceride levels, wherein experiment one refers to example 1, experiment two refers to example 2, and control refers to comparative example, as follows;
FIG. 2 is a graph of liver glycogen, liver glutathione peroxidase, malondialdehyde and total superoxide dismutase content;
FIG. 3 is a graph of liver oil red O staining sections.
Detailed Description
The invention is further described below with reference to examples and figures.
The preparation method of the composite additive in the embodiment of the invention comprises the following steps: 2 parts of N-carbamylglutamic acid, 4 parts of alpha-lipoic acid, 1 part of steroid saponin and 3 parts of chlorogenic acid are mixed according to a certain proportion.
Examples 1 to 2
The compound additive is prepared into the feed for the micropterus salmoides according to the additive amount of 1.5g/kg and 2.0g/kg, and the ingredients are shown in the table 1 according to percentage.
Table 1 feed ratios of example 1, example 2 and comparative example
Weighing materials in a formula, uniformly mixing, crushing solid raw materials, sieving with a 60-mesh sieve, mixing with a multidimensional and multi-mineral premix and an additive combination step by step, adding grease, adding 20kg of water per 100kg of feed mixture on the basis, kneading and forming the materials, putting the materials into a bulking machine, extruding the materials into particles, drying and packaging.
2. Experimental fish and aquaculture management
The experimental fish were young perches of the same year, and the young perches of the same weight of 6.02g were divided into 3 treatments, each treatment being repeated 3 times. Feeding for 2 times a day in the cultivation period, wherein the feeding time is respectively 8: 00. 17:00, and the cultivation period is 56 days.
By comparing the use method, the growth performance of the micropterus salmoides is shown in table 2:
table 2 growth performance of high starch feed additive composite additive
In the case of adding the composite additive into the high-starch feed, the example 1 and the example 2 show that the weight gain rate, the specific growth rate and the survival rate of the micropterus salmoides can be obviously improved, and the feed coefficient is reduced.
At the same time, by adding the composite additive, the serum triglyceride is obviously reduced (figure 1); by adding the complex additive, liver glutathione peroxidase and total superoxide dismutase enzyme activities were increased, and liver glycogen and malondialdehyde levels were decreased (fig. 2); by adding the complex additives, liver lipid deposition was significantly improved (fig. 3).
Although the foregoing embodiments have been described in some, but not all, embodiments of the invention, it should be understood that other embodiments may be devised in accordance with the present embodiments without departing from the spirit and scope of the invention.
Claims (9)
1. A composite additive for improving the utilization rate of high-starch feed by micropterus salmoides, which is characterized by comprising the following components: n-carbamylglutamic acid, alpha-lipoic acid, steroid saponin and chlorogenic acid.
2. The composite additive according to claim 1, wherein the composite additive comprises, in parts by weight: 2-6 parts of N-carbamylglutamic acid, 3-7 parts of alpha-lipoic acid, 1-3 parts of steroid saponin and 1-5 parts of chlorogenic acid.
3. The composite additive according to claim 1 or 2, characterized in that the composite additive comprises, in parts by weight: 2 parts of N-carbamylglutamic acid, 4 parts of alpha-lipoic acid, 1 part of steroid saponin and 3 parts of chlorogenic acid.
4. A method of preparing a composite additive according to any one of claims 1 to 3, comprising mixing the components.
5. Use of the compound additive according to any one of claims 1-3 for the preparation of a feed for micropterus salmoides.
6. A feed for a micropterus salmoides, characterized in that the feed for a micropterus salmoides contains the composite additive as defined in any one of claims 1 to 3.
7. The feed of claim 6, wherein the compound additive is added in an amount of 1.0-5.5 g/kg of feed.
8. The feed of claim 6 or 7, wherein the compound additive is added in an amount of 2.0g/kg of the feed.
9. The feed of claim 6 or 7, further comprising a high sugar feed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310726884.8A CN116508914A (en) | 2023-06-19 | 2023-06-19 | Composite additive and method for improving utilization rate of high-starch feed by micropterus salmoides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310726884.8A CN116508914A (en) | 2023-06-19 | 2023-06-19 | Composite additive and method for improving utilization rate of high-starch feed by micropterus salmoides |
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| Publication Number | Publication Date |
|---|---|
| CN116508914A true CN116508914A (en) | 2023-08-01 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310726884.8A Pending CN116508914A (en) | 2023-06-19 | 2023-06-19 | Composite additive and method for improving utilization rate of high-starch feed by micropterus salmoides |
Country Status (1)
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| CN (1) | CN116508914A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116998593A (en) * | 2023-09-15 | 2023-11-07 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Application of lactobacillus plantarum in preparation for improving fish sugar tolerance |
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| CN104000023A (en) * | 2014-05-07 | 2014-08-27 | 广东碧德生物科技有限公司 | Use of N-carbamylglutamic acid in preparation of preparation for reducing fish liver fat content or/and improving intramuscular fat content |
| CN107712456A (en) * | 2017-10-30 | 2018-02-23 | 佛山市高明通威饲料有限公司 | A kind of grisped grass carp feed and manufacture method |
| CN111011662A (en) * | 2019-12-24 | 2020-04-17 | 广西北海市旺海生物科技有限公司 | Compound feed for procypris merus |
| CN115024439A (en) * | 2022-06-21 | 2022-09-09 | 武汉大北农水产科技有限公司 | Functional premix for micropterus salmoides and preparation method thereof |
-
2023
- 2023-06-19 CN CN202310726884.8A patent/CN116508914A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104000023A (en) * | 2014-05-07 | 2014-08-27 | 广东碧德生物科技有限公司 | Use of N-carbamylglutamic acid in preparation of preparation for reducing fish liver fat content or/and improving intramuscular fat content |
| CN107712456A (en) * | 2017-10-30 | 2018-02-23 | 佛山市高明通威饲料有限公司 | A kind of grisped grass carp feed and manufacture method |
| CN111011662A (en) * | 2019-12-24 | 2020-04-17 | 广西北海市旺海生物科技有限公司 | Compound feed for procypris merus |
| CN115024439A (en) * | 2022-06-21 | 2022-09-09 | 武汉大北农水产科技有限公司 | Functional premix for micropterus salmoides and preparation method thereof |
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| Title |
|---|
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116998593A (en) * | 2023-09-15 | 2023-11-07 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Application of lactobacillus plantarum in preparation for improving fish sugar tolerance |
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