CN116495898A - Ecological restoration agent and water body treatment method - Google Patents
Ecological restoration agent and water body treatment method Download PDFInfo
- Publication number
- CN116495898A CN116495898A CN202310661384.0A CN202310661384A CN116495898A CN 116495898 A CN116495898 A CN 116495898A CN 202310661384 A CN202310661384 A CN 202310661384A CN 116495898 A CN116495898 A CN 116495898A
- Authority
- CN
- China
- Prior art keywords
- ecological restoration
- restoration agent
- graphene oxide
- water body
- washing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 44
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 22
- 239000010902 straw Substances 0.000 claims abstract description 33
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 29
- 229910021389 graphene Inorganic materials 0.000 claims abstract description 28
- 239000002131 composite material Substances 0.000 claims abstract description 18
- 102000004190 Enzymes Human genes 0.000 claims abstract description 17
- 108090000790 Enzymes Proteins 0.000 claims abstract description 17
- 239000004964 aerogel Substances 0.000 claims abstract description 14
- 102000002260 Alkaline Phosphatase Human genes 0.000 claims abstract description 13
- 108020004774 Alkaline Phosphatase Proteins 0.000 claims abstract description 13
- 108010029541 Laccase Proteins 0.000 claims abstract description 13
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 claims abstract description 13
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000000811 xylitol Substances 0.000 claims abstract description 13
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims abstract description 13
- 229960002675 xylitol Drugs 0.000 claims abstract description 13
- 235000010447 xylitol Nutrition 0.000 claims abstract description 13
- 239000000243 solution Substances 0.000 claims description 20
- 238000005406 washing Methods 0.000 claims description 18
- 238000006243 chemical reaction Methods 0.000 claims description 17
- 238000001914 filtration Methods 0.000 claims description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- 239000000843 powder Substances 0.000 claims description 12
- 238000001035 drying Methods 0.000 claims description 11
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 9
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 9
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 claims description 9
- 238000010438 heat treatment Methods 0.000 claims description 9
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 claims description 9
- 239000006185 dispersion Substances 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- 238000005273 aeration Methods 0.000 claims description 6
- 239000011259 mixed solution Substances 0.000 claims description 6
- NROKBHXJSPEDAR-UHFFFAOYSA-M potassium fluoride Chemical compound [F-].[K+] NROKBHXJSPEDAR-UHFFFAOYSA-M 0.000 claims description 6
- 230000007935 neutral effect Effects 0.000 claims description 5
- 238000004132 cross linking Methods 0.000 claims description 4
- 238000004108 freeze drying Methods 0.000 claims description 4
- 238000004321 preservation Methods 0.000 claims description 4
- 238000010992 reflux Methods 0.000 claims description 4
- 238000001179 sorption measurement Methods 0.000 claims description 4
- 229920001661 Chitosan Polymers 0.000 claims description 3
- BRLQWZUYTZBJKN-UHFFFAOYSA-N Epichlorohydrin Chemical compound ClCC1CO1 BRLQWZUYTZBJKN-UHFFFAOYSA-N 0.000 claims description 3
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 238000000498 ball milling Methods 0.000 claims description 3
- FOCAUTSVDIKZOP-UHFFFAOYSA-N chloroacetic acid Chemical compound OC(=O)CCl FOCAUTSVDIKZOP-UHFFFAOYSA-N 0.000 claims description 3
- 229940106681 chloroacetic acid Drugs 0.000 claims description 3
- 238000001027 hydrothermal synthesis Methods 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 239000011698 potassium fluoride Substances 0.000 claims description 3
- 235000003270 potassium fluoride Nutrition 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- 238000007710 freezing Methods 0.000 claims description 2
- 230000008014 freezing Effects 0.000 claims description 2
- XDRLEQYUIGRZLG-UHFFFAOYSA-M sodium;ethanol;hydroxide;hydrate Chemical compound O.[OH-].[Na+].CCO XDRLEQYUIGRZLG-UHFFFAOYSA-M 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 9
- 239000010865 sewage Substances 0.000 abstract description 8
- 244000005700 microbiome Species 0.000 description 15
- 229940088598 enzyme Drugs 0.000 description 14
- 239000000047 product Substances 0.000 description 14
- 235000010958 polyglycerol polyricinoleate Nutrition 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- 229910052698 phosphorus Inorganic materials 0.000 description 4
- 239000011574 phosphorus Substances 0.000 description 4
- 239000004593 Epoxy Substances 0.000 description 3
- 241000209140 Triticum Species 0.000 description 3
- 235000021307 Triticum Nutrition 0.000 description 3
- 238000010525 oxidative degradation reaction Methods 0.000 description 3
- 239000011265 semifinished product Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 108010093096 Immobilized Enzymes Proteins 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 238000000053 physical method Methods 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 229910052787 antimony Inorganic materials 0.000 description 1
- WATWJIUSRGPENY-UHFFFAOYSA-N antimony atom Chemical compound [Sb] WATWJIUSRGPENY-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- SOCTUWSJJQCPFX-UHFFFAOYSA-N dichromate(2-) Chemical compound [O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O SOCTUWSJJQCPFX-UHFFFAOYSA-N 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052750 molybdenum Inorganic materials 0.000 description 1
- 239000011733 molybdenum Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The invention relates to the technical field of ecological restoration, in particular to an ecological restoration agent and a water body treatment method, which comprise the following components in percentage by weight: 2 to 6: 0.5-1 percent of xylitol enzyme, alkaline phosphatase, laccase and graphene oxide/straw composite aerogel carrier, the ecological restoration agent has good treatment effect on river sewage, wherein COD Cr The removal rate is more than 70 percent, NH 3 The removal rate of N is more than 85 percent, the removal rate of TP is more than 50 percent, and the method is suitable for large-scale application.
Description
Technical Field
The invention relates to the technical field of ecological restoration, in particular to an ecological restoration agent and a water body treatment method.
Background
Due to the fact that urban sewage is discharged on two sides of a river channel, pollutants in the atmosphere, ground garbage and the like enter the river channel along with rainfall runoff flushing, the concentration of organic matters in the river channel is increased, the concentration of nitrogen and phosphorus is increased, the concentration of dissolved oxygen is reduced, aquatic animals and plants in the water body die, the self-cleaning capability of the water body is lost, and the water quality is deteriorated and smelly.
At present, river water treatment methods mainly comprise three types of physical methods, chemical methods and ecological restoration methods, wherein the physical methods comprise ecological water regulation, sediment dredging, garbage salvage and the like, but usually treat the symptoms and the root cause, the cost is higher, the chemical methods comprise medicament algae removal, flocculation precipitation and the like, secondary pollution is easy to cause, ecological unbalance can be caused by long-term use, the ecological restoration method has the advantages of no energy consumption or low energy consumption, low operation cost and the like, and is widely used at present, in the ecological restoration method, the removal of pollutants in water is mainly finished through the oxidative degradation of microorganisms, so the oxidative degradation capability of the microorganisms is directly related to the quality of the treatment effect.
At present, researches are focused on screening out microorganisms with higher degradation activity or compounding strains, and the symbiotic proliferation relationship is utilized to improve the oxidative degradation capability of the microorganisms, so that reports on water body treatment through enzyme preparations are few.
Disclosure of Invention
The invention aims to: aiming at the technical problems, the invention provides an ecological restoration agent and a water body treatment method.
The technical scheme adopted is as follows:
an ecological restoration agent comprises the following components in percentage by weight: 2 to 6: xylitol enzyme, alkaline phosphatase and laccase in 0.5-1 proportion.
Further, the composite aerogel further comprises a carrier, wherein the carrier is graphene oxide/straw composite aerogel.
Further, the preparation method of the graphene oxide/straw composite aerogel comprises the following steps:
mixing graphene oxide and straw powder, ball milling, dispersing in water, adding triethylamine to obtain a dispersion liquid, transferring the dispersion liquid into a hydrothermal reaction kettle, sealing and heating to 140-160 ℃ for reaction for 18-24 h, recovering room temperature, filtering, washing the obtained product, and freeze-drying at low temperature for 48-75 h.
Further, the weight ratio of the graphene oxide to the straw powder is 1:1 to 5.
Further, the graphene oxide is an epoxidized graphene oxide.
Further, the preparation method of the epoxidized graphene oxide comprises the following steps:
dispersing graphene oxide in DMF, adding potassium fluoride and potassium iodide, uniformly mixing, then dripping epichlorohydrin, heating to reflux reaction for 15-24 h after dripping, recovering room temperature, separating out a product, washing and drying.
Further, the straw powder is carboxylated straw powder.
Further, the preparation method of the carboxylated straw powder comprises the following steps:
crushing crop straws, drying, adding the crushed crop straws into a sodium hydroxide-ethanol-water composite solution, carrying out ultrasonic vibration dispersion, heating to 60-80 ℃, dropwise adding chloroacetic acid solution into the reaction solution, carrying out heat preservation reaction for 2-4 hours after dropwise adding, recovering the room temperature, filtering, washing the product with water, washing with sodium chloride solution and ethanol in sequence, and finally drying.
The invention also provides a preparation method of the ecological restoration agent, which comprises the following steps:
fixing xylitol enzyme, alkaline phosphatase and laccase on graphene oxide/straw composite aerogel by adopting a physical adsorption method, dispersing the graphene oxide/straw composite aerogel in water, mixing the graphene oxide/straw composite aerogel with acetic acid solution of chitosan to obtain mixed solution, dripping the mixed solution into sodium hydroxide solution, standing for 24-48 h after dripping, filtering, washing the obtained product until the product is neutral, adding the product into methanol, adding glutaraldehyde for crosslinking, filtering out and washing after 3-5 h, and finally freezing and drying at a low temperature.
The invention also provides a water body treatment method:
the ecological restoration agent is put into a water body, the water body is treated at the temperature of-5-40 ℃, and aeration is carried out on the water body while the treatment is carried out, so that the content of dissolved oxygen in the water body is ensured to be 1-10 mg/L.
The invention also discloses application of the ecological restoration agent in aquaculture, which can continuously play a role in purifying water areas in aquaculture, and the compound enzyme consisting of xylitol enzyme, alkaline phosphatase and laccase can selectively activate PGPR microorganisms in the water body, after the activation, the activity of the PGPR microorganisms is enhanced, the types are increased, the quantity is greatly improved, the characteristics of the PGPR are utilized to promote the decomposition of organic matters, purify water quality and restore ecology, and the ecological restoration agent has the effects of degrading eutrophic ammonia nitrogen and phosphorus, inhibiting the growth of harmful microorganisms, removing phosphorus and nitrogen from polluted water bodies, dredging organisms and the like.
The invention has the beneficial effects that:
the invention provides an ecological restoration agent, wherein, a composite enzyme composed of xylitol enzyme, alkaline phosphatase and laccase can selectively activate PGPR microorganisms in water, after the activation, the activity of the PGPR microorganisms is enhanced, the variety is increased, the quantity is greatly improved, the characteristics of the PGPR are utilized to promote the decomposition of organic matters, purify water quality and restore ecology, and the increase of the quantity and the activity of beneficial microorganisms are enhanced, so that the beneficial microorganisms establish population advantages, inhibit the growth and propagation of pathogenic bacteria, mould, viruses and other harmful microorganisms, thereby playing roles in regulating the balance of bacterial groups and inhibiting the growth of harmful microorganisms;
the graphene oxide/straw composite aerogel tape has carboxyl, epoxy and other reactive groups, and can be subjected to certain chemical bonding with enzyme in the immobilization process, so that the stability of the immobilized enzyme is improved, the physical adsorption and entrapment of the macroporous morphology of the immobilized enzyme are beneficial to the metabolic proliferation of PGPR microorganisms, excellent mass transfer performance is also presented, and the immobilized graphene/straw composite aerogel tape has excellent mechanical properties and large enzyme load capacity as a carrier due to the crosslinking of the epoxy and the carboxyl, and the straw can be used as an additional carbon source by microorganisms.
The ecological restoration agent has good treatment effect on river sewage, wherein COD Cr The removal rate is more than 70 percent, NH 3 The removal rate of N is more than 85 percent, the removal rate of TP is more than 50 percent, and the method is suitable for large-scale application.
Drawings
FIG. 1 is a comparison of the front and rear of the treatment of river sewage by the ecological restoration agent in the embodiment 1 of the present invention.
Detailed Description
The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention. The technology not mentioned in the present invention refers to the prior art, and unless otherwise indicated, the following examples and comparative examples are parallel tests, employing the same processing steps and parameters.
Example 1:
the preparation method of the ecological restoration agent comprises the following steps:
adding 500mL of DMF into a 1L flask with a charged stirrer, a thermometer and a reflux condenser, adding 2.2g of graphene oxide, 75.2g of potassium fluoride and 20g of potassium iodide, stirring and mixing uniformly, then dropwise adding 80mL of epichlorohydrin, heating to reflux reaction for 18h after dripping, recovering room temperature, filtering, repeatedly washing the obtained product with ethanol and deionized water, drying at 80 ℃ for 10h to obtain epoxidized graphene oxide, crushing 12g of wheat straw, putting the crushed wheat straw into a 200-mesh screen, drying at 100 ℃ for 24h in a baking oven, adding the crushed wheat straw into a composite solution consisting of 50g of sodium hydroxide, 375mL of ethanol and 125mL of water, ultrasonically oscillating for 30min, heating to 70 ℃, dropwise adding 200mL of chloroacetic acid solution with the mass fraction of 50% into the reaction solution, carrying out heat preservation reaction for 4 hours after dropwise adding, recovering the room temperature, filtering, washing a product to be neutral by using water, washing the product by using 5% of sodium chloride solution and ethanol in sequence, finally drying the product at 80 ℃ for 10 hours to obtain carboxylated straw powder, mixing 2g of epoxy graphene oxide and 8g of carboxylated straw powder, ball-milling for 2 hours, dispersing the mixture into 250mL of water, adding 5g of triethylamine to obtain a dispersion liquid, transferring the dispersion liquid into a hydrothermal reaction kettle, carrying out sealing heating to 150 ℃ for reaction for 24 hours, filtering after recovering the room temperature, washing the obtained product to be neutral by using water, and carrying out low-temperature freeze drying for 48 hours at-40 ℃ to obtain graphene oxide/straw composite aerogel; adding 0.4g of xylitol enzyme, 1g of alkaline phosphatase and 0.15g of laccase into 50mL of phosphate buffer solution, stirring for 15min, adding the graphene oxide/straw composite aerogel, carrying out heat preservation and adsorption for 60min, vacuum drying until the moisture content is 8%, obtaining a semi-finished product, dispersing the semi-finished product into 100mL of water, mixing the semi-finished product with 50mL of 2% acetic acid solution dissolved with 5g of chitosan to obtain a mixed solution, adding 150mL of 4% sodium hydroxide solution into the mixed solution, standing for 48h after dripping, filtering, washing the obtained product until the product is neutral, adding into 250mL of methanol, adding 1g of glutaraldehyde for crosslinking, filtering out after 4h, repeatedly washing with ethanol and deionized water, and finally carrying out low-temperature freeze drying for 24h at-40 ℃.
Example 2:
substantially the same as in example 1, except that 0.6g of xylitol enzyme, 1.2g of alkaline phosphatase and 0.2g of laccase were added.
Example 3:
substantially the same as in example 1, except that 0.2g of xylitol enzyme, 0.4g of alkaline phosphatase and 0.1g of laccase were added.
Example 4:
substantially the same as in example 1, except that 0.6g of xylitol enzyme, 0.4g of alkaline phosphatase and 0.2g of laccase were added.
Example 5:
substantially the same as in example 1, except that 0.2g of xylitol enzyme, 1.2g of alkaline phosphatase and 0.1g of laccase were added.
Comparative example 1:
substantially the same as in example 1, except that xylitol enzyme was not added.
Comparative example 2:
substantially the same as in example 1, except that alkaline phosphatase was not added.
Comparative example 3:
substantially the same as in example 1, except that no laccase was added.
Performance test:
river sewage is taken from a river in Hunan, and the water quality index is shown in table 1;
table 1:
| COD Cr /(mg/L) | NH 3 -N/(mg/L) | TP/(mg/L) | |
| river sewage | 185.3 | 32.8 | 6.9 |
Wherein, COD Cr The method is measured by adopting a dichromate oxidation method, and the specific reference standard GB-11914-89; NH (NH) 3 -N is determined by a nano reagent colorimetric method, specific reference standard GB 7479-87; the total phosphorus is measured by adopting a potassium persulfate oxidation-molybdenum antimony anti-spectrophotometry method, and the standard GB 11893-89 is specifically referred to.
The ecological restoration agent prepared in the examples 1-5 and the comparative examples 1-3 of the invention is used as a sample, an organic glass cylinder with the effective volume of 8L is used as a reaction vessel, river sewage is injected into the reaction vessel, a microporous aeration head is respectively added into the reaction vessel, the microporous aeration head is connected with an air compressor, the air compressor is started, an air inlet valve is regulated to ensure that the aeration amount in all the reaction vessels is basically consistent, the sample is put into the reaction vessel with the dosage of 0.15g/L, the water body is treated at room temperature, the operation is continued for 15d, aeration is carried out for 6h at the ratio of 10:00 to 16:00 per day, water quality detection is carried out after precipitation for 1h, and the removal rate (%) is calculated, and the results are shown in the table 2:
table 2:
as can be seen from the above Table 2, the ecological restoration agent of the invention has good treatment effect on river sewage, wherein COD Cr The removal rate is more than 70 percent, NH 3 The removal rate of N is more than 85 percent, the removal rate of TP is more than 50 percent, and the method is suitable for large-scale application.
The above embodiments are only for illustrating the technical solution of the present invention, and are not limiting; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims (10)
1. An ecological restoration agent is characterized by comprising the following components in percentage by weight: 2 to 6: xylitol enzyme, alkaline phosphatase and laccase in 0.5-1 proportion.
2. The ecological restoration agent according to claim 1, further comprising a carrier, wherein the carrier is graphene oxide/straw composite aerogel.
3. The ecological restoration agent as recited in claim 2, wherein the preparation method of the graphene oxide/straw composite aerogel is as follows:
mixing graphene oxide and straw powder, ball milling, dispersing in water, adding triethylamine to obtain a dispersion liquid, transferring the dispersion liquid into a hydrothermal reaction kettle, sealing and heating to 140-160 ℃ for reaction for 18-24 h, recovering room temperature, filtering, washing the obtained product, and freeze-drying at low temperature for 48-75 h.
4. The ecological restoration agent as recited in claim 3, wherein a weight ratio of said graphene oxide to straw powder is 1:1 to 5.
5. The ecological restoration agent according to claim 3, wherein said graphene oxide is an epoxidized graphene oxide.
6. The ecological restoration agent according to claim 5, wherein the preparation method of the epoxidized graphene oxide is as follows:
dispersing graphene oxide in DMF, adding potassium fluoride and potassium iodide, uniformly mixing, then dripping epichlorohydrin, heating to reflux reaction for 15-24 h after dripping, recovering room temperature, separating out a product, washing and drying.
7. The ecological restoration agent as defined in claim 3, wherein said straw powder is carboxylated straw powder.
8. The ecological restoration agent as defined in claim 7, wherein the preparation method of the carboxylated straw powder is as follows:
crushing crop straws, drying, adding the crushed crop straws into a sodium hydroxide-ethanol-water composite solution, carrying out ultrasonic vibration dispersion, heating to 60-80 ℃, dropwise adding chloroacetic acid solution into the reaction solution, carrying out heat preservation reaction for 2-4 hours after dropwise adding, recovering the room temperature, filtering, washing the product with water, washing with sodium chloride solution and ethanol in sequence, and finally drying.
9. The method for preparing the ecological restoration agent according to claim 2, which is characterized in that xylitol enzyme, alkaline phosphatase and laccase are fixed on graphene oxide/straw composite aerogel by adopting a physical adsorption method, dispersed in water, mixed with acetic acid solution of chitosan to obtain mixed solution, the mixed solution is added into sodium hydroxide solution in a liquid drop manner, standing for 24-48 h after the dripping, filtering, washing the obtained product until the product is neutral, adding into methanol, adding glutaraldehyde for crosslinking, filtering out and washing after 3-5 h, and finally freezing and drying at a low temperature.
10. A water body treatment method, which is characterized in that the ecological restoration agent of any one of claims 1 to 8 is put into a water body, the water body is treated at the temperature of-5 to 40 ℃, and aeration is carried out on the water body while the treatment is carried out, so that the content of dissolved oxygen in the water body is ensured to be 1 to 10mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310661384.0A CN116495898B (en) | 2023-06-06 | 2023-06-06 | Ecological restoration agent and water body treatment method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310661384.0A CN116495898B (en) | 2023-06-06 | 2023-06-06 | Ecological restoration agent and water body treatment method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116495898A true CN116495898A (en) | 2023-07-28 |
| CN116495898B CN116495898B (en) | 2023-11-03 |
Family
ID=87330409
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310661384.0A Active CN116495898B (en) | 2023-06-06 | 2023-06-06 | Ecological restoration agent and water body treatment method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116495898B (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104909467A (en) * | 2015-06-02 | 2015-09-16 | 陕西科技大学 | Bio-stimulant for biochemical treatment of paper-making wastewater |
| CN108636357A (en) * | 2018-06-07 | 2018-10-12 | 石梦成 | A kind of use in waste water treatment cross-linking type graphite oxide aerogel and preparation method thereof |
| CN109354217A (en) * | 2018-12-21 | 2019-02-19 | 江西省科学院 | A kind of river water body and bed mud comprehensive treatment agent and preparation method |
| CN110255810A (en) * | 2019-05-31 | 2019-09-20 | 广东五色时空生态环境科技有限公司 | Black-odor riverway biology in situ ecology synthesis intervenes sewage quality lifting process |
| CN111672432A (en) * | 2020-06-12 | 2020-09-18 | 西北大学 | Preparation method of graphene oxide/chitosan composite aerogel for enzyme immobilization |
| US20200298202A1 (en) * | 2017-12-12 | 2020-09-24 | Jiangsu Academy Of Agricultural Sciences | Preparation Method for Combined Modified Straw Active Particulate Carbon Adsorption Material and Use of Same |
| CN112159061A (en) * | 2020-09-07 | 2021-01-01 | 中国环境科学研究院 | Method for accurately positioning and in-situ treating pollutants on mud-water interface |
| CN113264633A (en) * | 2021-04-20 | 2021-08-17 | 广西壮族自治区环境保护科学研究院 | Ecological restoration method for black and odorous river channel |
| CN114871266A (en) * | 2022-04-29 | 2022-08-09 | 天津城建大学 | Method for repairing soil polluted by refractory organic matters by using immobilized laccase in cooperation with soil microbial electrochemical system |
-
2023
- 2023-06-06 CN CN202310661384.0A patent/CN116495898B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104909467A (en) * | 2015-06-02 | 2015-09-16 | 陕西科技大学 | Bio-stimulant for biochemical treatment of paper-making wastewater |
| US20200298202A1 (en) * | 2017-12-12 | 2020-09-24 | Jiangsu Academy Of Agricultural Sciences | Preparation Method for Combined Modified Straw Active Particulate Carbon Adsorption Material and Use of Same |
| CN108636357A (en) * | 2018-06-07 | 2018-10-12 | 石梦成 | A kind of use in waste water treatment cross-linking type graphite oxide aerogel and preparation method thereof |
| CN109354217A (en) * | 2018-12-21 | 2019-02-19 | 江西省科学院 | A kind of river water body and bed mud comprehensive treatment agent and preparation method |
| CN110255810A (en) * | 2019-05-31 | 2019-09-20 | 广东五色时空生态环境科技有限公司 | Black-odor riverway biology in situ ecology synthesis intervenes sewage quality lifting process |
| CN111672432A (en) * | 2020-06-12 | 2020-09-18 | 西北大学 | Preparation method of graphene oxide/chitosan composite aerogel for enzyme immobilization |
| CN112159061A (en) * | 2020-09-07 | 2021-01-01 | 中国环境科学研究院 | Method for accurately positioning and in-situ treating pollutants on mud-water interface |
| CN113264633A (en) * | 2021-04-20 | 2021-08-17 | 广西壮族自治区环境保护科学研究院 | Ecological restoration method for black and odorous river channel |
| CN114871266A (en) * | 2022-04-29 | 2022-08-09 | 天津城建大学 | Method for repairing soil polluted by refractory organic matters by using immobilized laccase in cooperation with soil microbial electrochemical system |
Also Published As
| Publication number | Publication date |
|---|---|
| CN116495898B (en) | 2023-11-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112340853B (en) | Hybrid hydrogel carrier for high-salinity wastewater treatment and preparation method thereof | |
| CN105132342B (en) | A kind of sanitary sewage disposal GS microbial inoculums and its application | |
| CN108359663B (en) | Phosphorus-accumulating bacterium immobilized pellet and application thereof | |
| CN108410769A (en) | Fresh water complex microorganism bottom changes the preparation method of agent | |
| CN103374561A (en) | Preparation and water treatment using method of immobilized nitrifying bacteria | |
| CN105274025B (en) | A kind of composite flora and its treatment process of heavy metal-polluted water process | |
| CN110982735A (en) | Biochar-based microbial inoculant and preparation method and application thereof | |
| CN113462396A (en) | Biochar soil conditioner and preparation method and application thereof | |
| CN110724683A (en) | Carbon-based microbial composite bacteria powder, microbial inoculum and preparation method and application thereof | |
| CN107473404B (en) | Water purifying agent with self-formed block-shaped carbon carrier for fixing microorganisms and preparation method thereof | |
| CN115846374A (en) | Method for preparing carbon source for wastewater treatment by utilizing kitchen waste | |
| CN116495898B (en) | Ecological restoration agent and water body treatment method | |
| CN101816920B (en) | Modified phanerochaete chrysosporium adsorbent as well as preparation and application thereof | |
| CN105820976A (en) | Sludge compost thermophilic microbial agent and application | |
| CN103285825B (en) | Method for increasing yield of heavy metal bio-adsorbent | |
| CN116656552A (en) | Application of pseudo-intermediate brucella in preparation of microbial inoculum for efficiently adsorbing and converting heavy metals | |
| CN101629148B (en) | Microorganism heavy metal precipitator and preparation method thereof | |
| CN106754532A (en) | A kind of composite bacteria agent that surface active agent wastewater is rich in for degrading and preparation method thereof | |
| CN114149087B (en) | Method for treating arsenic-containing waste liquid by agricultural waste in cooperation with microorganisms | |
| CN113711751B (en) | Facility cultivation water and fertilizer integrated system and fertilizer application method | |
| CN112410245B (en) | Low-temperature composting microbial composite microbial agent and preparation method and application thereof | |
| CN108249585B (en) | Composite biological agent for treating polluted river and preparation method thereof | |
| CN111925965B (en) | Biological sewage treatment composite microbial inoculum and preparation method thereof | |
| CN109964765B (en) | Weissella-based rice cadmium content reduction method | |
| CN112375721A (en) | Preparation method and application of composite microbial inoculum for low-temperature treatment of rural domestic sewage |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |