CN116473081A - Microbial agent and foliar fertilizer - Google Patents
Microbial agent and foliar fertilizer Download PDFInfo
- Publication number
- CN116473081A CN116473081A CN202310460327.6A CN202310460327A CN116473081A CN 116473081 A CN116473081 A CN 116473081A CN 202310460327 A CN202310460327 A CN 202310460327A CN 116473081 A CN116473081 A CN 116473081A
- Authority
- CN
- China
- Prior art keywords
- active ingredient
- microbial agent
- trichoderma harzianum
- thymol
- natamycin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 24
- 230000000813 microbial effect Effects 0.000 title claims abstract description 21
- 239000003337 fertilizer Substances 0.000 title claims abstract description 12
- 241000223260 Trichoderma harzianum Species 0.000 claims abstract description 48
- MGSRCZKZVOBKFT-UHFFFAOYSA-N thymol Chemical compound CC(C)C1=CC=C(C)C=C1O MGSRCZKZVOBKFT-UHFFFAOYSA-N 0.000 claims abstract description 41
- 239000004480 active ingredient Substances 0.000 claims abstract description 36
- 239000005844 Thymol Substances 0.000 claims abstract description 21
- 229960000790 thymol Drugs 0.000 claims abstract description 21
- 229960003255 natamycin Drugs 0.000 claims abstract description 19
- 235000010298 natamycin Nutrition 0.000 claims abstract description 19
- 239000004311 natamycin Substances 0.000 claims abstract description 19
- NCXMLFZGDNKEPB-FFPOYIOWSA-N natamycin Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/C[C@@H](C)OC(=O)/C=C/[C@H]2O[C@@H]2C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 NCXMLFZGDNKEPB-FFPOYIOWSA-N 0.000 claims abstract description 18
- AVXURJPOCDRRFD-UHFFFAOYSA-N Hydroxylamine Chemical compound ON AVXURJPOCDRRFD-UHFFFAOYSA-N 0.000 claims abstract description 16
- CRDAMVZIKSXKFV-FBXUGWQNSA-N (2-cis,6-cis)-farnesol Chemical compound CC(C)=CCC\C(C)=C/CC\C(C)=C/CO CRDAMVZIKSXKFV-FBXUGWQNSA-N 0.000 claims abstract description 14
- 239000000260 (2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-ol Substances 0.000 claims abstract description 13
- 229940043259 farnesol Drugs 0.000 claims abstract description 13
- 229930002886 farnesol Natural products 0.000 claims abstract description 13
- CRDAMVZIKSXKFV-UHFFFAOYSA-N trans-Farnesol Natural products CC(C)=CCCC(C)=CCCC(C)=CCO CRDAMVZIKSXKFV-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000005788 Fluxapyroxad Substances 0.000 claims abstract description 9
- SXSGXWCSHSVPGB-UHFFFAOYSA-N fluxapyroxad Chemical compound FC(F)C1=NN(C)C=C1C(=O)NC1=CC=CC=C1C1=CC(F)=C(F)C(F)=C1 SXSGXWCSHSVPGB-UHFFFAOYSA-N 0.000 claims abstract description 9
- 101000623895 Bos taurus Mucin-15 Proteins 0.000 claims abstract description 3
- 241000918585 Pythium aphanidermatum Species 0.000 claims description 5
- 240000008067 Cucumis sativus Species 0.000 claims description 4
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims description 4
- 208000031888 Mycoses Diseases 0.000 claims description 4
- 240000007594 Oryza sativa Species 0.000 claims description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims description 3
- 206010039509 Scab Diseases 0.000 claims description 3
- 235000009566 rice Nutrition 0.000 claims description 3
- 240000007124 Brassica oleracea Species 0.000 claims description 2
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 claims description 2
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 claims description 2
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 claims description 2
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 2
- 206010027146 Melanoderma Diseases 0.000 claims description 2
- 241000233679 Peronosporaceae Species 0.000 claims description 2
- 241000221662 Sclerotinia Species 0.000 claims description 2
- 240000003768 Solanum lycopersicum Species 0.000 claims description 2
- 241000209140 Triticum Species 0.000 claims description 2
- 235000021307 Triticum Nutrition 0.000 claims description 2
- 240000008042 Zea mays Species 0.000 claims description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 2
- 235000005822 corn Nutrition 0.000 claims description 2
- 235000013619 trace mineral Nutrition 0.000 claims description 2
- 239000011573 trace mineral Substances 0.000 claims description 2
- 239000002054 inoculum Substances 0.000 claims 1
- 239000003814 drug Substances 0.000 abstract description 12
- 230000002195 synergetic effect Effects 0.000 abstract description 10
- 241000894006 Bacteria Species 0.000 abstract description 6
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 6
- 206010059866 Drug resistance Diseases 0.000 abstract description 5
- 150000001875 compounds Chemical class 0.000 abstract description 5
- 230000000857 drug effect Effects 0.000 abstract description 3
- 230000002035 prolonged effect Effects 0.000 abstract description 3
- 230000002829 reductive effect Effects 0.000 abstract description 2
- 241000123650 Botrytis cinerea Species 0.000 description 13
- 230000001580 bacterial effect Effects 0.000 description 6
- 201000010099 disease Diseases 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 230000012010 growth Effects 0.000 description 6
- 230000004071 biological effect Effects 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 244000052616 bacterial pathogen Species 0.000 description 4
- 239000000575 pesticide Substances 0.000 description 4
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 3
- 241000223218 Fusarium Species 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 239000010413 mother solution Substances 0.000 description 3
- OILXMJHPFNGGTO-UHFFFAOYSA-N (22E)-(24xi)-24-methylcholesta-5,22-dien-3beta-ol Natural products C1C=C2CC(O)CCC2(C)C2C1C1CCC(C(C)C=CC(C)C(C)C)C1(C)CC2 OILXMJHPFNGGTO-UHFFFAOYSA-N 0.000 description 2
- RQOCXCFLRBRBCS-UHFFFAOYSA-N (22E)-cholesta-5,7,22-trien-3beta-ol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CCC(C)C)CCC33)C)C3=CC=C21 RQOCXCFLRBRBCS-UHFFFAOYSA-N 0.000 description 2
- OQMZNAMGEHIHNN-UHFFFAOYSA-N 7-Dehydrostigmasterol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CC(CC)C(C)C)CCC33)C)C3=CC=C21 OQMZNAMGEHIHNN-UHFFFAOYSA-N 0.000 description 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- DNVPQKQSNYMLRS-NXVQYWJNSA-N Ergosterol Natural products CC(C)[C@@H](C)C=C[C@H](C)[C@H]1CC[C@H]2C3=CC=C4C[C@@H](O)CC[C@]4(C)[C@@H]3CC[C@]12C DNVPQKQSNYMLRS-NXVQYWJNSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 241000747105 Fuscoporia Species 0.000 description 2
- 102000019259 Succinate Dehydrogenase Human genes 0.000 description 2
- 108010012901 Succinate Dehydrogenase Proteins 0.000 description 2
- 239000003899 bactericide agent Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000013329 compounding Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- DNVPQKQSNYMLRS-SOWFXMKYSA-N ergosterol Chemical compound C1[C@@H](O)CC[C@]2(C)[C@H](CC[C@]3([C@H]([C@H](C)/C=C/[C@@H](C)C(C)C)CC[C@H]33)C)C3=CC=C21 DNVPQKQSNYMLRS-SOWFXMKYSA-N 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 2
- 229920000053 polysorbate 80 Polymers 0.000 description 2
- 230000029058 respiratory gaseous exchange Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 229940124186 Dehydrogenase inhibitor Drugs 0.000 description 1
- 239000005760 Difenoconazole Substances 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 239000005794 Hymexazol Substances 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 241001558929 Sclerotium <basidiomycota> Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000000843 anti-fungal effect Effects 0.000 description 1
- 229940121375 antifungal agent Drugs 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 201000009361 ascariasis Diseases 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000006013 carbendazim Substances 0.000 description 1
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- BQYJATMQXGBDHF-UHFFFAOYSA-N difenoconazole Chemical compound O1C(C)COC1(C=1C(=CC(OC=2C=CC(Cl)=CC=2)=CC=1)Cl)CN1N=CN=C1 BQYJATMQXGBDHF-UHFFFAOYSA-N 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 235000021186 dishes Nutrition 0.000 description 1
- 230000037149 energy metabolism Effects 0.000 description 1
- 244000053095 fungal pathogen Species 0.000 description 1
- KGVPNLBXJKTABS-UHFFFAOYSA-N hymexazol Chemical compound CC1=CC(O)=NO1 KGVPNLBXJKTABS-UHFFFAOYSA-N 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000003120 macrolide antibiotic agent Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 230000009965 odorless effect Effects 0.000 description 1
- 230000000361 pesticidal effect Effects 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 150000004291 polyenes Chemical class 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 125000002328 sterol group Chemical group 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000009967 tasteless effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 230000004102 tricarboxylic acid cycle Effects 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/38—Trichoderma
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N49/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds containing the group, wherein m+n>=1, both X together may also mean —Y— or a direct carbon-to-carbon bond, and the carbon atoms marked with an asterisk are not part of any ring system other than that which may be formed by the atoms X, the carbon atoms in square brackets being part of any acyclic or cyclic structure, or the group, wherein A means a carbon atom or Y, n>=0, and not more than one of these carbon atoms being a member of the same ring system, e.g. juvenile insect hormones or mimics thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Zoology (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Chemical & Material Sciences (AREA)
- Dentistry (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Virology (AREA)
- Insects & Arthropods (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of microbial agents, and particularly relates to a microbial agent and a foliar fertilizer. The effective components of the microbial agent are compounded by an active component I and an active component II according to a certain proportion; the active ingredient I is trichoderma harzianum, and the active ingredient II is farnesol, fluxapyroxad hydroxylamine, thymol or natamycin. According to the invention, trichoderma harzianum is compounded with the existing antibacterial and bactericidal active ingredients, and the compound combination with synergistic effect is screened, so that the drug effect on target bacteria can be improved, and the service life of the medicament can be prolonged. And simultaneously, the drug resistance risk existing when a single active ingredient is used can be reduced.
Description
Technical Field
The invention belongs to the technical field of microbial agents, and particularly relates to a microbial agent and a foliar fertilizer.
Background
Trichoderma harzianum is a microbial bactericide produced by Bayer GmbH in the United states, can inhibit the growth of bacteria, can also produce compounds for stimulating the growth of plants and inducing the defense reaction of the plants, enhances the growth vigor and disease resistance of the plants, and improves the crop yield and income. Application number CN201510697474.0 discloses that the combination of trichoderma harzianum and hymexazol has a synergistic effect on rice damping-off, can improve the pesticide effect and reduce the pesticide use amount and the pesticide cost.
Farnesol, formula C 15 H 26 O, CAS:4602-84-0. Application number CN201810759419.3 discloses application of farnesol as a sterilization synergist in plant gray mold control, and in the patent, the application discloses that the farnesol and difenoconazole or carbendazim have a synergistic effect on gray mold inhibition when being combined.
Fluxapyroxad hydroxylamine is the latest generation succinate dehydrogenase inhibitor bactericide discovered and developed by the first da, and can inhibit the growth of pathogenic bacteria and cause the death of the pathogenic bacteria by inhibiting succinate dehydrogenase in tricarboxylic acid cycle in the process of respiration of pathogenic bacteria mitochondria to influence the respiration of pathogenic fungi, block energy metabolism. Can be used for preventing and treating various diseases including gray mold, brown spot, leaf spot, sclerotinia and scab.
Thymol is present in essential oils of various plants and is mostly used for preparing spice, preservative, medical sterilization and ascariasis-expelling agent, and recent researches show that thymol has strong effect of inhibiting hypha growth on botrytis cinerea and cotton damping-off pathogenic bacteria.
Natamycin is a polyene macrolide antifungal antibiotic, which is a crystalline powder with a molecular formula of C, which is almost odorless and tasteless from being colored to milky 33 H 47 NO 13 The molecular weight is 665.73. Natamycin (R)The element has strong inhibiting or killing effect on filamentous fungi such as saccharomycetes and mould, and has the action mechanism of combining with ergosterol and other sterol groups of the fungi to block the ergosterol so as to distort cell membranes, and finally lead to leakage and cell death.
In the pesticide control of diseases and insect pests, when a single active ingredient medicament is used for disease for a long time, target bacteria easily generate drug resistance, so that the drug effect is not ideal. Similarly, disease control is carried out by long-term dependence on trichoderma harzianum, and target bacteria have drug resistance, and the drug resistance is improved and the service life of the drug is prolonged by compounding the target bacteria with other active ingredient drugs and screening a compound formula with synergistic effect. At present, no report is found on the compounding of trichoderma harzianum with farnesol, fluxapyroxad hydroxylamine, thymol or natamycin.
Disclosure of Invention
The invention aims to provide a microbial agent and a foliar fertilizer, which are used for solving the problem existing in the process of disease control by depending on trichoderma harzianum for a long time.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the effective components of the microbial agent are compounded by an active component I and an active component II according to a certain proportion; the active ingredient I is trichoderma harzianum, and the active ingredient II is farnesol, fluxapyroxad hydroxylamine, thymol or natamycin.
More specifically, the trichoderma harzianum is 300 hundred million CFU/g trichoderma harzianum.
More specifically, when the active ingredient II is farnesol, the mass ratio of the active ingredient I to the active ingredient II is 1-40:15-1.
More specifically, when the active ingredient II is fluxapyroxad hydroxylamine, the mass ratio of the active ingredient I to the active ingredient II is 1-3:9-1.
More specifically, when the active ingredient II is thymol, the mass ratio of the active ingredient I to the active ingredient II is 1-16:20-1.
More specifically, when the active ingredient II is natamycin, the mass ratio of the active ingredient I to the active ingredient II is 1-29:8-1.
The invention also provides a foliar fertilizer which comprises the microbial agent and a fertilizer component, wherein the fertilizer component comprises one or more of N, P, K or trace elements.
The invention also provides the application of the microbial agent or the foliar fertilizer in preventing and controlling crop fungal diseases.
More specifically, the fungal diseases of crops include tomato gray mold, cucumber downy mildew, wheat scab, rice damping off, cucumber fusarium wilt, rape sclerotium disease, cabbage black spot, apple ring rot and corn leaf spot.
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, trichoderma harzianum is compounded with the existing antibacterial and bactericidal active ingredients, and the compound combination with synergistic effect is screened, so that the drug effect on target bacteria can be improved, and the service life of the medicament can be prolonged. And simultaneously, the drug resistance risk existing when a single active ingredient is used can be reduced.
Detailed Description
The following description of the embodiments of the present invention will be apparent from, and is intended to provide a thorough description of, the embodiments of the present invention, and not a complete description of, the embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, are intended to fall within the scope of the present invention.
Examples: indoor biological Activity assay
1. Test strain: botrytis cinerea Pers. Fr.) is isolated from Guangxi cortex Lin Shi in the field, and stored on PDA medium.
2. Test agent
300 hundred million CFU/g Trichoderma harzianum (accession number: PD 20140320), 95% farnesol (Shanghai Yuan Yes Biotechnology Co., ltd.), 98% fluxapyroxal hydroxylamine (accession number: PD 20212312), 98% thymol (Shanghai Yuan Yes Biotechnology Co., ltd.), 99% natamycin (Nantong Yingruida Biotechnology Co., ltd.)
Preparing trichoderma harzianum into a suspension mother solution by using sterile water, dissolving the rest of test agents by using dimethyl sulfoxide, and diluting by using 0.1% Tween-80 aqueous solution to prepare a single-agent mother solution; then a plurality of groups of proportions are arranged, and each single-dose mother solution and the proportion mixture are provided with 5 mass concentration gradients according to the content of the effective components.
3. Test method
The hypha growth rate method is adopted. Under the aseptic condition, uniformly mixing 5mL of prepared liquid medicine with 45mL of pre-melted culture medium, and then pouring the mixture into 5 culture dishes with the diameter of 9cm in an equivalent manner to prepare a medicine-containing flat plate with corresponding concentration; a blank control was prepared by mixing an equal amount of 0.1% Tween-80 in water with the culture medium, and 3 replicates were performed for each concentration of the drug solution. Cutting bacterial cakes with the diameter of 5mm from the edges of bacterial colonies of the cultured Botrytis cinerea under the aseptic condition by using a puncher, inoculating the bacterial cakes to the centers of a medicine-containing flat plate and a blank control flat plate, placing the bacterial colonies in a constant temperature box at 25 ℃ for culturing for 3d with the mycelium facing downwards, measuring the diameters of the bacterial colonies by using a crisscross method for each time, taking the average value of the bacterial colonies, and calculating the inhibition rate of different treatments on the growth of the mycelium.
4. Data analysis: data statistical analysis is carried out by adopting DPS software to obtain a virulence regression equation and EC 50 Values and co-toxicity coefficients (CTCs) were calculated according to the grand cloud Pei method.
The synergy of the agents was evaluated based on the calculated co-toxicity coefficient (CTC), CTC.ltoreq.80 being antagonism, CTC.ltoreq.120 being additive, CTC.ltoreq.120 being synergy, the results being shown in tables 1-4.
TABLE 1 determination of indoor biological Activity of Trichoderma harzianum and farnesol Complex against Botrytis cinerea
As shown in Table 1, after the Trichoderma harzianum and the farnesol are compounded, the mass ratio is 1-40: the co-toxicity coefficient of the botrytis cinerea within the range of 15-1 is more than 120, and the synergistic effect is shown.
TABLE 2 determination of indoor biological Activity of Trichoderma harzianum and Fusarium on Botrytis cinerea by Fuscoporia
| Medicament name and ratio | EC50(mg/L) | ATI | TTI | CTC |
| Trichoderma harzianum | 13.9115 | 100.0000 | -- | -- |
| Fluoxapyroxad hydroxylamine | 0.8681 | 1602.5228 | -- | -- |
| Trichoderma harzianum 1: fluoxapyroxad hydroxylamine 9 | 0.7483 | 1859.0806 | 1452.2705 | 128.0120 |
| Trichoderma harzianum 1: fluoxazoyl hydroxylamine 7 | 0.6463 | 2152.4834 | 1414.7074 | 152.1504 |
| Trichoderma harzianum 1: fluoxazoyl hydroxylamine 3 | 0.9108 | 1527.3935 | 1226.8921 | 124.4929 |
| Trichoderma harzianum 1: fluoxapyroxad hydroxylamine 1 | 1.1328 | 1228.0632 | 851.2614 | 144.2639 |
| Trichoderma harzianum 3: fluoxapyroxad hydroxylamine 1 | 2.4297 | 572.5604 | 475.6307 | 120.3792 |
As shown in table 2, after the trichoderma harzianum and the fluxapyroxad hydroxylamine are compounded, the mass ratio is 1-3: the co-toxicity coefficient of the botrytis cinerea within the range of 9-1 is more than 120, and the synergistic effect is shown.
TABLE 3 determination of indoor biological Activity of Trichoderma harzianum and thymol complex against Botrytis cinerea
| Medicament name and ratio | EC50(mg/L) | ATI | TTI | CTC |
| Trichoderma harzianum | 13.9115 | 100.0000 | -- | -- |
| Thymol | 16.2377 | 85.6741 | -- | -- |
| Trichoderma harzianum 1: thymol 20 | 13.0627 | 106.4979 | 86.3563 | 123.3239 |
| Trichoderma harzianum 1: thymol 15 | 11.8290 | 117.6050 | 86.5694 | 135.8505 |
| Trichoderma harzianum 1: thymol 7 | 9.3804 | 148.3039 | 87.4648 | 169.5584 |
| Trichoderma harzianum 1: thymol 5 | 7.5622 | 183.9610 | 88.0617 | 208.9001 |
| Trichoderma harzianum 1: thymol 1 | 12.3802 | 112.3689 | 92.8370 | 121.0389 |
| Trichoderma harzianum 5: thymol 1 | 10.8666 | 128.0207 | 97.6123 | 131.1522 |
| Trichoderma harzianum 7: thymol 1 | 5.1406 | 270.6202 | 98.2093 | 275.5546 |
| Trichoderma harzianum 16: thymol 1 | 3.2164 | 432.5177 | 99.1573 | 436.1935 |
As shown in table 3, after the trichoderma harzianum and thymol are compounded, the mass ratio is 1-16: the co-toxicity coefficient of the botrytis cinerea within the range of 20-1 is more than 120, and the synergistic effect is shown.
TABLE 4 determination of indoor biological Activity of Trichoderma harzianum and Fusarium on Botrytis cinerea by Fuscoporia
| Medicament name and ratio | EC50(mg/L) | ATI | TTI | CTC |
| Trichoderma harzianum | 13.9115 | 100.0000 | -- | -- |
| Natamycin | 5.3387 | 260.5784 | -- | -- |
| Trichoderma harzianum 1: natamycin 8 | 4.7104 | 295.3359 | 242.7364 | 121.6694 |
| Trichoderma harzianum 1: natamycin 5 | 3.6715 | 378.9051 | 233.8153 | 162.0531 |
| Trichoderma harzianum 1: natamycin 1 | 6.3963 | 217.4929 | 180.2892 | 120.6356 |
| Trichoderma harzianum 5: natamycin 1 | 8.0529 | 172.7514 | 126.7631 | 136.2790 |
| Trichoderma harzianum 7: natamycin 1 | 8.9995 | 154.5808 | 120.0723 | 128.7398 |
| Trichoderma harzianum 15: natamycin 1 | 7.2007 | 193.1965 | 110.0362 | 175.5755 |
| Trichoderma harzianum 29: natamycin 1 | 10.0451 | 138.4904 | 105.3526 | 131.4542 |
As shown in table 4, after the trichoderma harzianum and the natamycin are compounded, the mass ratio is 1-29: the co-toxicity coefficient of the botrytis cinerea within the range of 8-1 is more than 120, and the synergistic effect is shown.
In conclusion, after the trichoderma harzianum, the farnesol, the fluxapyroxad hydroxylamine, the thymol or the natamycin are compounded, the compound has a good synergistic effect on the botrytis cinerea, can improve the control effect of the botrytis cinerea, is beneficial to reducing the pesticide application dosage and pesticide residues.
Claims (9)
1. The microbial agent is characterized in that the effective components of the microbial agent are compounded by an active component I and an active component II according to a certain proportion; the active ingredient I is trichoderma harzianum, and the active ingredient II is farnesol, fluxapyroxad hydroxylamine, thymol or natamycin.
2. The microbial inoculant of claim 1, wherein the trichoderma harzianum is 300 hundred million CFU/gram trichoderma harzianum.
3. The microbial agent according to claim 1, wherein when the active ingredient II is farnesol, the mass ratio of the active ingredient I to the active ingredient II is 1-40:15-1.
4. The microbial agent according to claim 1, wherein when the active ingredient II is fluxapyroxad hydroxylamine, the mass ratio of the active ingredient I to the active ingredient II is 1-3:9-1.
5. The microbial agent according to claim 1, wherein when the active ingredient II is thymol, the mass ratio of the active ingredient I to the active ingredient II is 1-16:20-1.
6. The microbial agent according to claim 1, wherein when the active ingredient II is natamycin, the mass ratio of the active ingredient I to the active ingredient II is 1-29:8-1.
7. A foliar fertilizer comprising the microbial agent of any one of claims 1 to 6 and a fertilizer component comprising one or more of N, P, K or trace elements.
8. Use of the microbial agent of any one of claims 1 to 6 or the foliar fertilizer of claim 7 for controlling fungal diseases of crops.
9. The use according to claim 8, wherein the crop fungal disease comprises tomato gray mold, cucumber downy mildew, wheat scab, rice damping off, cucumber damping off, rape sclerotinia, cabbage black spot, apple ring rot and corn big spot.
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