CN113383778A - Validamycin-containing bactericidal composition - Google Patents
Validamycin-containing bactericidal composition Download PDFInfo
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- CN113383778A CN113383778A CN202110591158.0A CN202110591158A CN113383778A CN 113383778 A CN113383778 A CN 113383778A CN 202110591158 A CN202110591158 A CN 202110591158A CN 113383778 A CN113383778 A CN 113383778A
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- validamycin
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
Abstract
The invention discloses a validamycin-containing bactericidal composition, which contains active ingredients of validamycin and nano-silver, wherein the weight ratio of the validamycin to the nano-silver is 60: 1-1:60. Further discloses the application of the compound in preventing and treating rice sheath blight disease. The validamycin and the nano-silver are compounded together, the action mechanisms of the validamycin and the nano-silver are different, the compounding and the synergism are obvious, the pesticide effect can be greatly improved, the pesticide use amount and the pesticide cost are reduced, meanwhile, the lasting period of the validamycin is prolonged, the control effect of chemical pesticides which is comparable to that of a perfect chemical pesticide is achieved, and the advantages of micro toxicity and even no toxicity of biological pesticides are achieved.
Description
Technical Field
The invention belongs to the technical field of pesticide application, and particularly relates to a validamycin-containing bactericidal composition.
Background
The rice sheath blight disease is one of the main diseases of rice, and is also called as leaf streak disease, commonly known as flower and stalk, rotten foot plague and eyebrow spot. The rhizoctonia solani is infected with diseases, the grain filling of rice plants which are affected slightly under high-temperature and high-humidity conditions is affected, the lodging and the death can be caused seriously, and the yield of rice can be affected seriously if the control is not carried out in time.
Validamycin, also known as validamycin, is produced by actinomycetesThe raw antibiotic has strong systemic property, is easy to be absorbed by the somatic cells and quickly conducted in the somatic cells, and interferes and inhibits the growth and development of the somatic cells. Belongs to a low-toxicity bactericide. Pure oral LD for big mouse50Are all more than 2000 mg/kg, and are injected subcutaneously with LD50Are all more than 1500 mg/kg. 5000 mg/kg of the composition is applied to the skin of a rat without toxic reaction. Low toxicity to fish, carp TLM (96h) LD50>40 mg/l. Although validamycin has a good effect of preventing rice sheath blight, validamycin is easily degraded in a natural environment due to the fact that the validamycin is aminoglycoside antibiotics, and the effective duration of the validamycin is only about seven days, so that the further use of the validamycin is limited.
The nano silver is prepared by utilizing the leading edge nano technology to carry out nano treatment on the silver, few nano silver can generate a strong bactericidal effect, 650 kinds of bacteria can be killed within a few minutes, the nano silver is broad-spectrum bactericidal and has no drug resistance, wound healing, cell growth and damaged cell repair can be promoted, no toxic reaction exists, no stimulation reaction is found on skin, a wide prospect is opened for widely applying the nano silver to resist bacteria, the nano silver is a latest generation of natural antibacterial agent, nano silver particles directly enter thalli to be combined with oxygen metabolizing enzyme (-SH), a unique action mechanism for enabling the thalli to be killed by asphyxiation is achieved, and most of bacteria, fungi, moulds, spores and other microorganisms which are contacted with the nano silver can be killed.
Validamycin and nano-silver are used as biological pesticides, have the advantage of low toxicity, but have obvious defects, the validamycin has short duration for preventing and treating rice sheath blight, and the nano-silver has poor drug efficacy for preventing and treating rice sheath blight, so that the share of chemical pesticides in the drug for preventing and treating rice sheath blight is increasingly larger, but the chemical pesticides have higher toxicity than the biological pesticides and influence food safety, and therefore, the development of a biological bactericide with good effect, low toxicity and long duration is urgently needed.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to provide the sterilization composition of the crythromycin with high simulation, long lasting period and low toxicity.
The invention is realized by the following technical scheme:
the validamycin-containing bactericidal composition is characterized by comprising the active ingredients of validamycin and nano-silver, wherein the weight ratio of the validamycin to the nano-silver is 60: 1-1:60.
Preferably, the weight ratio of the validamycin to the nano silver is 30: 1-1:30.
Preferably, the weight ratio of validamycin to nano silver is 20: 1-1:20.
Preferably, the weight ratio of the validamycin to the nano silver is 10: 1-1:10.
Preferably, the weight ratio of the validamycin to the nano silver is 1: 1.
The validamycin-containing bactericidal composition is applied to prevention and treatment of rice sheath blight disease.
The validamycin and the nano-silver are compounded together, the action mechanisms of the validamycin and the nano-silver are different, the compounding and the synergism are obvious, the pesticide effect can be greatly improved, the pesticide use amount and the pesticide cost are reduced, meanwhile, the lasting period of the validamycin is prolonged, the control effect of chemical pesticides which is comparable to that of a perfect chemical pesticide is achieved, and the advantages of micro toxicity and even no toxicity of biological pesticides are achieved.
Detailed Description
The present invention will be further described in detail with reference to the following specific examples to better understand the technical solution.
The percentages of the invention are weight percentages.
The embodiment of the invention adopts a method combining indoor toxicity measurement and field test.
Example 1 indoor toxicity determination of Validamycin combinations on Rice sheath blight disease
Wherein, the rice sheath blight germ is collected in the field and is purified and cultured indoors.
The test method comprises the following steps: after the effective inhibition concentration range of each medicament is determined by pre-test, the nano-silver, validamycin and a mixed preparation thereof are serially diluted into 5 concentrations according to the content gradient of effective components, sterile water is used as a contrast, a hypha growth rate method is adopted for determination, medicaments with different concentrations are added into a sterilized culture dish, 1mL of each dish is added, 9mL of sterilized and melted PDA culture medium is added, the mixture is fully and uniformly mixed to prepare a flat plate, the expanded bacterial colony is made into a bacterial cake of 0.5cm, each dish is repeatedly treated for 3 times, the flat plate is placed in a constant temperature box at the temperature of 28 ℃ for culture, the diameter of the bacterial colony is measured for 4 days, the hypha growth inhibition rate is calculated, the toxicity regression formula is calculated, and the EC50 is calculated, wherein the formula of the:
hypha growth inhibition (%) (blank colony diameter-agent-treated colony diameter)/blank colony diameter 100
The synergistic effect of the medicine mixture is evaluated by a Sun Yunpei co-toxicity coefficient (CTC) method, namely CTC <80 is antagonistic action, CTC <80 >120 is additive action, and CTC >120 is synergistic action.
The results of the indoor toxicity test of the composition containing validamycin and nano-silver on rice sheath blight disease are shown in Table 1
TABLE 1 indoor toxicity test table for Validamycin and nano-silver compounded to rice sheath blight pathogenic bacteria
| Medicament | EC50(mg/L) | Measured virulence index | Theoretical coefficient of merit | Co-toxicity coefficient |
| Validamycin A | 26.81 | 100 | - | - |
| Nano silver (B) | 33.05 | 81.12 | - | - |
| A：B＝60:1 | 21.66 | 123.78 | 99.69 | 124.16 |
| A：B＝30:1 | 21.82 | 122.87 | 99.39 | 123.62 |
| A：B＝20:1 | 21.73 | 123.38 | 99.10 | 124.50 |
| A：B＝10:1 | 21.37 | 125.46 | 98.28 | 127.65 |
| A：B＝1:1 | 18.20 | 147.31 | 90.56 | 162.66 |
| A：B＝1:10 | 23.64 | 113.41 | 82.84 | 136.90 |
| A：B＝1:20 | 24.98 | 107.33 | 82.02 | 130.85 |
| A：B＝1:30 | 25.91 | 103.47 | 81.73 | 126.66 |
| A：B＝1:60 | 26.24 | 102.17 | 81.43 | 125.47 |
As can be seen from Table 1, the EC of validamycin and nano-silver for rice sheath blight disease5026.81mg/L and 33.05mg/L respectively, when the mass ratio of the validamycin to the nano-silver is 1:60-60:1, the co-toxicity coefficient is more than 120, and the synergy is shown, and when the mass ratio of the validamycin to the nano-silver is 1:1, the co-toxicity coefficient is the largest and the synergy effect is the best.
Example 2
The validamycin and the nano-silver are compounded according to the weight ratio of 60:1 for field test of rice sheath blight prevention, different medicaments are prepared into corresponding concentrations according to the requirements of the following table, the medicaments are applied for the first time in the full tillering stage of rice and for the second time after 7 days, the application is carried out twice, the disease index is measured 7 days, 14 days and 21 days after the application, the drug effect is calculated, and the result is shown in the table 2.
TABLE 2 comparison table of the efficacy of controlling rice sheath blight
(data from 2020 field efficacy test)
As can be seen from table 2, the field efficacy test results show that, under the test conditions of example 2, the validamycin and nano-silver composition has excellent control effect on rice sheath blight disease, has synergistic effect compared with a single agent in control effect, and can reduce the usage amount of pesticide and reduce the cost.
Example 3
Compounding validamycin and nano-silver according to a weight ratio of 30:1, performing a field test of preventing and treating rice sheath blight, preparing different medicaments into corresponding concentrations according to the requirements of the following table, applying the medicaments for the first time in the tillering stage of rice and for the second time after 7 days, applying the medicaments for two times, measuring disease indexes 7 days, 14 days and 21 days after application, and calculating the medicament effect, wherein the results are shown in table 3.
TABLE 3 comparison of the efficacy of controlling rice sheath blight
(data from 2020 field efficacy test)
As can be seen from table 3, the field efficacy test results show that, under the test conditions of example 3, the validamycin and nano-silver composition has an excellent control effect on rice sheath blight disease, and the control effect has a synergistic effect compared with a single agent, so that the usage amount of the pesticide can be reduced, and the cost can be reduced.
Example 4
Compounding validamycin and nano-silver according to a weight ratio of 20:1, performing a field test for preventing and controlling rice sheath blight, preparing different medicaments into corresponding concentrations according to the requirements of the following table, applying the medicaments for the first time in the full tillering stage of rice, applying the medicaments for the second time after 7 days, measuring disease indexes 7 days, 14 days and 21 days after application, and calculating the pesticide effect, wherein the results are shown in the table 4.
TABLE 4 comparison table of the efficacy of controlling rice sheath blight
(data from 2020 field efficacy test)
As can be seen from table 4, the field efficacy test results show that, under the test conditions of example 4, the validamycin and nano-silver composition has an excellent control effect on rice sheath blight disease, and the control effect has a synergistic effect compared with a single agent, so that the usage amount of the pesticide can be reduced, and the cost can be reduced.
Example 5
Compounding validamycin and nano-silver according to a weight ratio of 10:1, performing a field test for preventing and controlling rice sheath blight, preparing different medicaments into corresponding concentrations according to the requirements of the following table, applying the medicaments for the first time in the full tillering stage of rice, applying the medicaments for the second time after 7 days, measuring disease indexes 7 days, 14 days and 21 days after application, and calculating the pesticide effect, wherein the results are shown in the table 5.
TABLE 5 comparison table of the efficacy of controlling rice sheath blight
(data from 2020 field efficacy test)
As can be seen from table 5, the field efficacy test results show that, under the test conditions of example 5, the validamycin and nano-silver composition has excellent control effect on rice sheath blight disease, has synergistic effect compared with a single agent in control effect, and can reduce the usage amount of pesticide and reduce the cost.
Example 6
Compounding validamycin and nano-silver according to the weight ratio of 1:1, performing a field test for preventing and controlling rice sheath blight, preparing different medicaments into corresponding concentrations according to the requirements of the following table, applying the medicaments for the first time in the full tillering stage of rice, applying the medicaments for the second time after 7 days, measuring disease indexes and calculating the pesticide effect after 7 days, 14 days and 21 days after the pesticide application, wherein the calculated pesticide effect result is shown in table 6.
TABLE 6 comparison of the efficacy of controlling rice sheath blight
(data from 2020 field efficacy test)
As can be seen from table 6, the field efficacy test results show that, under the test conditions of example 6, the validamycin and nano-silver composition has excellent control effect on rice sheath blight disease, has synergistic effect compared with a single agent in control effect, and can reduce the usage amount of pesticide and reduce the cost.
Example 7
Compounding validamycin and nano-silver according to a weight ratio of 1:10, performing a field test for preventing and controlling rice sheath blight, preparing different medicaments into corresponding concentrations according to the requirements of the following table, applying the medicaments for the first time in the full tillering stage of rice, applying the medicaments for the second time after 7 days, measuring disease indexes 7 days, 14 days and 21 days after application, and calculating the pesticide effect, wherein the results are shown in the table 7.
TABLE 7 comparison of the efficacy of controlling rice sheath blight
(data from 2020 field efficacy test)
As can be seen from table 7, the results of the field efficacy tests show that, under the test conditions of example 7, the validamycin and nano-silver composition has excellent control effect on rice sheath blight disease, has synergistic effect compared with a single agent in control effect, and can reduce the usage amount of pesticide and reduce the cost.
Example 8
Compounding validamycin and nano-silver according to a weight ratio of 1:20, performing a field test for preventing and controlling rice sheath blight, preparing different medicaments into corresponding concentrations according to the requirements of the following table, applying the medicaments for the first time in the full tillering stage of rice, applying the medicaments for the second time after 7 days, measuring disease indexes 7 days, 14 days and 21 days after application, and calculating the pesticide effect, wherein the results are shown in the table 8.
TABLE 8 comparison table of the efficacy of controlling rice sheath blight
(data from 2020 field efficacy test)
As can be seen from table 8, the field efficacy test results show that, under the test conditions of example 8, the validamycin and nano-silver composition has excellent control effect on rice sheath blight disease, has synergistic effect compared with a single agent in control effect, and can reduce the usage amount of pesticide and reduce the cost.
Example 9
Compounding validamycin and nano-silver according to a weight ratio of 1:30, performing a field test on rice sheath blight, preparing different medicaments into corresponding concentrations according to the requirements of the following table, applying the medicaments for the first time in the full tillering stage of rice and applying the medicaments for the second time after 7 days, measuring disease indexes and calculating the drug effect after 7 days, 14 days and 21 days after application, wherein the results are shown in the table 9.
TABLE 9 comparison table of the efficacy of controlling rice sheath blight
(data from 2020 field efficacy test)
As can be seen from table 9, the field efficacy test results show that, under the test conditions of example 9, the validamycin and nano-silver composition has excellent control effect on rice sheath blight disease, has synergistic effect compared with a single agent in control effect, and can reduce the usage amount of pesticide and reduce the cost.
Example 10
Compounding validamycin and nano-silver according to a weight ratio of 1:60, performing a field test for preventing and controlling rice sheath blight, preparing different medicaments into corresponding concentrations according to the requirements of the following table, applying the medicaments for the first time in the full tillering stage of rice, applying the medicaments for the second time after 7 days, measuring disease indexes 7 days, 14 days and 21 days after application, and calculating the pesticide effect, wherein the results are shown in the table 10.
TABLE 10 comparison of the efficacy of controlling rice sheath blight
(data from 2020 field efficacy test)
As can be seen from table 10, the field efficacy test results show that, under the test conditions of example 10, the validamycin and nano-silver composition has an excellent control effect on rice sheath blight disease, and the control effect has a synergistic effect compared with a single agent, so that the usage amount of the pesticide can be reduced, and the cost can be reduced.
Claims (6)
1. The validamycin-containing bactericidal composition is characterized by comprising active ingredients of validamycin and nano-silver, wherein the weight ratio of the validamycin to the nano-silver is 60: 1-1:60.
2. The bactericidal composition containing validamycin as claimed in claim 1, wherein the weight ratio of the validamycin to the nano silver is 30: 1-1:30.
3. The bactericidal composition containing validamycin as claimed in claim 1, wherein the weight ratio of the validamycin to the nano silver is 20: 1-1:20.
4. The bactericidal composition containing validamycin as claimed in claim 1, wherein the weight ratio of the validamycin to the nano silver is 10: 1-1:10.
5. The bactericidal composition containing validamycin as claimed in claim 1, wherein the weight ratio of the validamycin to the nano silver is 1: 1.
6. An application of a validamycin-containing bactericidal composition in preventing and treating rice sheath blight disease.
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| CN108522544A (en) * | 2018-05-22 | 2018-09-14 | 浙江省桐庐汇丰生物科技有限公司 | A kind of bactericidal composition and its application containing jinggangmeisu |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108522544A (en) * | 2018-05-22 | 2018-09-14 | 浙江省桐庐汇丰生物科技有限公司 | A kind of bactericidal composition and its application containing jinggangmeisu |
Non-Patent Citations (1)
| Title |
|---|
| 马汇泉等: "纳米银制备及提高生防菌剂抗菌活性", 《农药》 * |
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