CN116459318A - Preparation method of gastrodia elata hydrolysate - Google Patents
Preparation method of gastrodia elata hydrolysate Download PDFInfo
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- CN116459318A CN116459318A CN202310608878.2A CN202310608878A CN116459318A CN 116459318 A CN116459318 A CN 116459318A CN 202310608878 A CN202310608878 A CN 202310608878A CN 116459318 A CN116459318 A CN 116459318A
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- gastrodia elata
- rhizoma gastrodiae
- hydrolysate
- gastrodin
- pulp
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- 241000305491 Gastrodia elata Species 0.000 title claims abstract description 111
- 239000000413 hydrolysate Substances 0.000 title claims abstract description 36
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 239000002002 slurry Substances 0.000 claims abstract description 19
- 238000001914 filtration Methods 0.000 claims abstract description 17
- 238000001035 drying Methods 0.000 claims abstract description 16
- 238000011033 desalting Methods 0.000 claims abstract description 15
- 108010009736 Protein Hydrolysates Proteins 0.000 claims abstract description 13
- 230000003301 hydrolyzing effect Effects 0.000 claims abstract description 13
- 239000003513 alkali Substances 0.000 claims abstract description 12
- 230000001105 regulatory effect Effects 0.000 claims abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 32
- 239000002994 raw material Substances 0.000 claims description 25
- 239000000084 colloidal system Substances 0.000 claims description 16
- 239000000843 powder Substances 0.000 claims description 16
- 239000008213 purified water Substances 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 10
- 238000004537 pulping Methods 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 9
- 238000003801 milling Methods 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 2
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- 229930193974 gastrodin Natural products 0.000 abstract description 47
- PUQSUZTXKPLAPR-NZEXEKPDSA-N helicidol Natural products O([C@H]1[C@H](O)[C@H](O)[C@@H](O)[C@@H](CO)O1)c1ccc(CO)cc1 PUQSUZTXKPLAPR-NZEXEKPDSA-N 0.000 abstract description 47
- 239000000047 product Substances 0.000 abstract description 9
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- YSCJAYPKBYRXEZ-HZPINHDXSA-N (2s,3s,4s,5r,6r)-6-[[(3s,4ar,6ar,6bs,8as,12as,14ar,14br)-4,4,6a,6b,11,11,14b-heptamethyl-8a-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxycarbonyl-1,2,3,4a,5,6,7,8,9,10,12,12a,14,14a-tetradecahydropicen-3-yl]oxy]-3-hydroxy-4-[(2s,3r,4s, Chemical compound O([C@H]1[C@H](O)[C@H](O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@H]1CC[C@]2(C)[C@H]3CC=C4[C@@]([C@@]3(CC[C@H]2C1(C)C)C)(C)CC[C@]1(CCC(C[C@H]14)(C)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)C(O)=O)[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O YSCJAYPKBYRXEZ-HZPINHDXSA-N 0.000 description 1
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- MQSVACYEBUOKAY-AEAHGDTJSA-N Periplocoside N Natural products O([C@@H](C)[C@]1(O)[C@]2(C)[C@H]([C@H]3[C@@H]([C@]4(C)C(=CC3)C[C@@H](O)CC4)CC2)CC1)[C@H]1O[C@@H](C)[C@@H](O)[C@@H](O)C1 MQSVACYEBUOKAY-AEAHGDTJSA-N 0.000 description 1
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 1
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- KPJWZUAARPJYSB-UHFFFAOYSA-N glycoside C Natural products CC1(C)OC(=O)C23CCC1C2(O)CCC(C1(CCC24)C)(C)C3CCC1C2(C)CCCC4(C)COC(C(C(O)C1O)OC2C(C(O)C(CO)O2)O)OC1COC1OC(CO)C(O)C(O)C1O KPJWZUAARPJYSB-UHFFFAOYSA-N 0.000 description 1
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
- A61K36/8988—Gastrodia
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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Abstract
The invention discloses a preparation method of gastrodia elata hydrolysate, and belongs to the technical field of plant extraction. Which comprises the following steps: (1) making rhizoma Gastrodiae into rhizoma Gastrodiae slurry; (2) Regulating pH of rhizoma Gastrodiae slurry to 8-12 with alkali solution, hydrolyzing at 30-80deg.C for 30-200 min to obtain rhizoma Gastrodiae hydrolysate; (3) Filtering the rhizoma Gastrodiae hydrolysate, purifying, desalting, concentrating, and drying to obtain rhizoma Gastrodiae hydrolysate. According to the invention, by effectively hydrolyzing some special components which are not gastrodin in the gastrodia elata, such as gastrodin precursors of the gastrodia elata such as the gastrodia elata Li Sengan, in the conventional extraction and separation process under the alkaline condition, the hydrolysate with higher gastrodin content is obtained, and compared with the content of free gastrodin in raw gastrodia elata, the level of free gastrodin in the gastrodia elata product can be remarkably improved.
Description
Technical Field
The invention relates to a preparation method of gastrodia elata hydrolysate, and belongs to the technical field of plant extraction.
Background
Gastrodia elata is a traditional rare traditional Chinese medicine, is loaded in Chinese pharmacopoeia (2015 edition one), is clinically used for treating headache, dizziness, limb numbness and other symptoms, has pharmacological effects of protecting nerves, sedating and hypnotizing, enhancing immunity and the like, and is mainly produced in regions such as Yunnan, guizhou, hubei and Sichuan. With the development of modern phytochemistry, research shows that gastrodin is the most main pharmacological component in gastrodia elata, the content of which is generally 0.33% -0.67%, and the content of which is also up to 1.5%. The gastrodin has wide pharmacological effects, such as sedation and hypnosis, intelligence promotion, neuron protection, blood pressure reduction, convulsion resistance, epilepsy resistance, oxidation resistance, aging resistance, pain relief, microcirculation improvement and the like, is safe, has no toxic or side effect, and the gastrodin related preparations or medicines are widely used for treating cardiovascular and cerebrovascular diseases and neuropsychiatric diseases clinically, such as headache, coronary heart disease, hypertension, post-cycle ischemic dizziness, sudden deafness, epilepsy, neurasthenia, cerebral apoplexy and the like.
Gastrodine is the most important pharmacological component in rhizoma Gastrodiae, and in rhizoma Gastrodiae product, the higher the gastrodine content is, the better the drug effect is, but the less gastrodine content in rhizoma Gastrodiae. At present, the way of obtaining gastrodin is mainly divided into artificial synthesis and natural extraction and separation, and the solvent residue and safety of the artificial synthesis product are questioned by people, and the extraction and separation yield from the gastrodia elata is lower. In the past, how to safely and efficiently improve the level of free gastrodin in a gastrodia elata product is a technical problem to be solved urgently by a person skilled in the art.
Disclosure of Invention
The invention aims to provide a preparation method of a gastrodia elata hydrolysate, which is characterized in that a gastrodia elata precursor which is not gastrodin in conventional extraction and separation processes, such as ba Li Sengan and the like, is effectively hydrolyzed under alkaline conditions to obtain a hydrolysate with high gastrodin content, and compared with the content of free gastrodin in raw gastrodia elata, the level of free gastrodin in a gastrodia elata product can be obviously improved.
In order to achieve the above purpose, the invention is realized by the following technical scheme:
a preparation method of rhizoma Gastrodiae hydrolysate comprises the following steps:
(1) Preparing gastrodia elata pulp: taking dry gastrodia elata as a raw material, crushing the dry gastrodia elata into gastrodia elata powder, and mixing the gastrodia elata powder with the following raw materials in mass: adding purified water in the ratio of (10-20) (m: v) to water=1, and then grinding with a colloid mill to obtain gastrodia elata pulp; or cleaning fresh rhizoma Gastrodiae, pulping with a pulping machine, and mixing with rhizoma Gastrodiae according to the weight ratio: purified water is added in the proportion of (4-8) (m: v) water=1, and then the gastrodia elata pulp is prepared by colloid milling.
(2) Hydrolysis: adding alkali liquor into the gastrodia elata pulp, adjusting the pH of the gastrodia elata pulp to 8-12, heating to 30-80 ℃, hydrolyzing at constant temperature for 30-200 minutes, and adjusting the pH to be neutral after the hydrolysis is completed to obtain gastrodia elata hydrolysate;
(3) Filtering the rhizoma Gastrodiae hydrolysate, purifying, desalting, concentrating, and drying to obtain rhizoma Gastrodiae hydrolysate.
During filtration, the gastrodia elata hydrolysate is treated by a conventional filtration method to remove sediment and particles, the filtrate is treated by an ultrafiltration membrane to remove macromolecular substances such as protein, starch and the like, and then the filtrate is desalted by a nanofiltration membrane to remove monovalent ions to reduce salt content, and finally the gastrodia elata hydrolysate is obtained by concentration and drying.
Preferably, in the step (2), alkali liquor is used for adjusting the pH value of the gastrodia elata pulp to 11.5-12. Experiments show that under the condition that other conditions are consistent, the pH value of the gastrodia elata pulp is controlled in the range during hydrolysis, and the gastrodin yield is higher.
Preferably, the temperature in the step (2) is controlled to be 50-60 ℃. Experiments show that under the condition that other conditions are consistent, the temperature of the gastrodia elata pulp is controlled in the range during hydrolysis, and the gastrodin yield is higher.
Further, the determination of gastrodin in the present invention: the gastrodin content is extracted and measured according to the method of the pharmacopoeia of the people's republic of China.
The technical principle of the invention is as follows: the gastrodia elata contains a large amount of gastrodin precursor substance components such as the balrison glycoside A, the balrison glycoside B, the balrison glycoside C, the balrison glycoside E and the like, and the number of the balrison glycoside compounds found in the gastrodia elata reaches more than 30. The balison glycoside is a special compound formed by condensing gastrodin and citric acid, and the precursor substances are not gastrodin during conventional extraction and separation. According to the invention, researches show that some special components in gastrodia elata, such as the barrison glycoside compounds, can be hydrolyzed under an alkaline condition, and gastrodin, citric acid and other small molecular components are formed after hydrolysis, so that the gastrodin level is obviously improved.
The invention has the beneficial effects that:
(1) The invention adopts a hydrolysis method with mild conditions, the production process is nontoxic and harmless, the used equipment is simple, and the environment is not polluted; by effectively hydrolyzing some special components which are not gastrodin in the gastrodia elata, such as gastrodin precursors of the gastrodia elata Li Sengan and the like, in the conventional extraction and separation process under the alkaline condition, a hydrolysate with higher gastrodin content is obtained, and compared with the content of free gastrodin in the gastrodia elata serving as a raw material, the level of free gastrodin in the gastrodia elata product can be remarkably improved.
(2) The gastrodia elata hydrolysate and hydrolysate obtained by the invention can be further converted into health-care food and medical products, and raw materials with higher gastrodin content are provided for the fields of health-care food and medicine; meanwhile, the product with high gastrodin content can play a faster and better medical care effect.
(3) The method is simple and convenient to operate, and can be used for rapidly detecting the change of the gastrodin by using a standard HPLC method.
(4) The gastrodia elata hydrolysate prepared by the method is a product, can be further prepared into gastrodia elata oral liquid, gastrodia elata candy, purified gastrodin and other products, and can be further purified by combining a pharmaceutical chemistry method to obtain high-purity gastrodin.
Detailed Description
In order to make the objects, technical solutions and advantageous effects of the present invention more apparent, preferred embodiments of the present invention will be described in detail below to facilitate understanding by the skilled person.
The invention is further illustrated by the following specific examples, but the scope of the invention is not limited to the description.
Example 1:
(1) Preparing rhizoma Gastrodiae hydrolysate: taking dry gastrodia elata as a raw material, crushing the dry gastrodia elata into gastrodia elata powder, and mixing the gastrodia elata powder with the following raw materials in mass: adding purified water in a ratio of water=1:10 (m: v), and then grinding with a colloid mill to obtain rhizoma Gastrodiae pulp; adding sodium hydroxide solution into rhizoma Gastrodiae slurry, adjusting pH to 8, heating to 30deg.C, maintaining the temperature for 30 min for constant temperature hydrolysis, and adjusting pH to neutrality with hydrochloric acid to obtain hydrolyzed primary slurry; treating hydrolyzed pulp by conventional filtration method to remove precipitate and particulates, treating filtrate with ultrafiltration membrane to remove macromolecular substances such as protein and starch, desalting with nanofiltration membrane to remove monovalent ions and reduce salt content to obtain rhizoma Gastrodiae hydrolysate, concentrating and drying to obtain rhizoma Gastrodiae hydrolysate. And measuring gastrodin.
(2) Preparation of a non-hydrolytic control: taking dry gastrodia elata as a raw material, crushing the dry gastrodia elata into gastrodia elata powder, and mixing the gastrodia elata powder with the following raw materials in mass: adding purified water in a ratio of water=1:10 (m: v), and then grinding with a colloid mill to obtain rhizoma Gastrodiae pulp; directly heating rhizoma Gastrodiae pulp to 30deg.C without alkali adjustment, and maintaining for 30 min; treating the heat-preserved rhizoma Gastrodiae slurry by conventional filtration method to remove precipitate and particulates, treating the filtrate with ultrafiltration membrane to remove macromolecular substances such as protein and starch, desalting with nanofiltration membrane to remove monovalent ions and reduce salt content, concentrating and drying the filtrate to obtain rhizoma Gastrodiae extract. And measuring gastrodin.
Example 2:
(1) Preparing rhizoma Gastrodiae hydrolysate: taking dry gastrodia elata as a raw material, crushing the dry gastrodia elata into gastrodia elata powder, and mixing the gastrodia elata powder with the following raw materials in mass: adding purified water in a ratio of water=1:15 (m: v), and then grinding with a colloid mill to obtain rhizoma Gastrodiae pulp; adding potassium hydroxide solution into rhizoma Gastrodiae pulp, adjusting pH to 10, heating to 55deg.C, maintaining for 90 min for constant temperature hydrolysis, and adjusting pH to neutrality to obtain hydrolyzed pulp; treating hydrolyzed pulp by conventional filtration method to remove precipitate and particulates, treating filtrate with ultrafiltration membrane to remove macromolecular substances such as protein and starch, desalting with nanofiltration membrane to remove monovalent ions and reduce salt content to obtain rhizoma Gastrodiae hydrolysate, concentrating and drying to obtain rhizoma Gastrodiae hydrolysate. And measuring gastrodin.
(2) Preparation of a non-hydrolytic control: taking dry gastrodia elata as a raw material, crushing the dry gastrodia elata into gastrodia elata powder, and mixing the gastrodia elata powder with the following raw materials in mass: adding purified water in a ratio of water=1:15 (m: v), and then grinding with a colloid mill to obtain rhizoma Gastrodiae pulp; directly heating rhizoma Gastrodiae pulp to 55deg.C without alkali adjustment, and maintaining for 90 min; treating the heat-preserved rhizoma Gastrodiae slurry by conventional filtration method to remove precipitate and particulates, treating the filtrate with ultrafiltration membrane to remove macromolecular substances such as protein and starch, desalting with nanofiltration membrane to remove monovalent ions and reduce salt content, concentrating and drying the filtrate to obtain rhizoma Gastrodiae extract. And measuring gastrodin.
Example 3:
(1) Preparing rhizoma Gastrodiae hydrolysate: taking dry gastrodia elata as a raw material, crushing the dry gastrodia elata into gastrodia elata powder, and mixing the gastrodia elata powder with the following raw materials in mass: adding purified water in a ratio of water=1:20 (m: v), and then grinding with a colloid mill to obtain rhizoma Gastrodiae pulp; adding food-grade alkali liquor into rhizoma Gastrodiae pulp, adjusting pH of rhizoma Gastrodiae pulp to 12, heating to 80deg.C, maintaining for 200 min for constant temperature hydrolysis, and adjusting pH to neutrality to obtain hydrolyzed pulp; treating hydrolyzed pulp by conventional filtration method to remove precipitate and particulates, treating filtrate with ultrafiltration membrane to remove macromolecular substances such as protein and starch, desalting with nanofiltration membrane to remove monovalent ions and reduce salt content to obtain rhizoma Gastrodiae hydrolysate, concentrating and drying to obtain rhizoma Gastrodiae hydrolysate. And measuring gastrodin.
(2) Preparation of a non-hydrolytic control: taking dry gastrodia elata as a raw material, crushing the dry gastrodia elata into gastrodia elata powder, and mixing the gastrodia elata powder with the following raw materials in mass: adding purified water in a ratio of water=1:20 (m: v), and then grinding with a colloid mill to obtain rhizoma Gastrodiae pulp; directly heating rhizoma Gastrodiae pulp to 80deg.C without alkali regulation, and maintaining for 200 min; treating the heat-preserved rhizoma Gastrodiae slurry by conventional filtration method to remove precipitate and particulates, treating the filtrate with ultrafiltration membrane to remove macromolecular substances such as protein and starch, desalting with nanofiltration membrane to remove monovalent ions and reduce salt content, concentrating and drying the filtrate to obtain rhizoma Gastrodiae extract. And measuring gastrodin.
Example 4:
(1) Preparing rhizoma Gastrodiae hydrolysate: the fresh gastrodia elata is taken as a raw material, cleaned, pulped by a pulping machine, and the fresh gastrodia elata is prepared according to the mass of the fresh gastrodia elata: purified water was added in a ratio of water=1:4 (m: v), followed by milling with a colloid mill; obtaining gastrodia elata pulp; adding sodium hydroxide solution into rhizoma Gastrodiae slurry, adjusting pH to 8, heating to 30deg.C, maintaining the temperature for 30 min for constant temperature hydrolysis, and adjusting pH to neutrality to obtain hydrolyzed primary slurry; treating hydrolyzed pulp by conventional filtration method to remove precipitate and particulates, treating filtrate with ultrafiltration membrane to remove macromolecular substances such as protein and starch, desalting with nanofiltration membrane to remove monovalent ions and reduce salt content to obtain rhizoma Gastrodiae hydrolysate, concentrating and drying to obtain rhizoma Gastrodiae hydrolysate. And measuring gastrodin.
(2) Preparation of a non-hydrolytic control: the fresh gastrodia elata is taken as a raw material, cleaned, pulped by a pulping machine, and the fresh gastrodia elata is prepared according to the mass of the fresh gastrodia elata: adding purified water in a ratio of water=1:4 (m: v), and then grinding with a colloid mill to obtain rhizoma Gastrodiae pulp; directly heating rhizoma Gastrodiae slurry to 30deg.C without adding alkali liquor, and maintaining for 30 min; treating the heat-preserved rhizoma Gastrodiae slurry by conventional filtration method to remove precipitate and particulates, treating the filtrate with ultrafiltration membrane to remove macromolecular substances such as protein and starch, desalting with nanofiltration membrane to remove monovalent ions and reduce salt content to obtain rhizoma Gastrodiae extractive solution, concentrating and drying to obtain rhizoma Gastrodiae extract. And measuring gastrodin.
Example 5:
(1) Preparing rhizoma Gastrodiae hydrolysate: the fresh gastrodia elata is taken as a raw material, cleaned, pulped by a pulping machine, and the fresh gastrodia elata is prepared according to the mass of the fresh gastrodia elata: purified water was added in a ratio of water=1:8 (m: v), followed by milling with a colloid mill; obtaining gastrodia elata pulp; adding sodium hydroxide solution into rhizoma Gastrodiae pulp, adjusting pH to 10, heating to 50deg.C, maintaining the temperature for 100 min for constant temperature hydrolysis, and adjusting pH to neutrality to obtain hydrolyzed pulp; treating hydrolyzed pulp by conventional filtration method to remove precipitate and particulates, treating filtrate with ultrafiltration membrane to remove macromolecular substances such as protein and starch, desalting with nanofiltration membrane to remove monovalent ions and reduce salt content to obtain rhizoma Gastrodiae hydrolysate, concentrating and drying to obtain rhizoma Gastrodiae hydrolysate. And measuring gastrodin.
(2) Preparation of a non-hydrolytic control: the fresh gastrodia elata is taken as a raw material, cleaned, pulped by a pulping machine, and the fresh gastrodia elata is prepared according to the mass of the fresh gastrodia elata: adding purified water in a ratio of water=1:8 (m: v), and then grinding with a colloid mill to obtain rhizoma Gastrodiae pulp; directly heating rhizoma Gastrodiae slurry to 50deg.C without adding alkali liquor, and maintaining for 100 min; treating the heat-preserved rhizoma Gastrodiae slurry by conventional filtration method to remove precipitate and particulates, treating the filtrate with ultrafiltration membrane to remove macromolecular substances such as protein and starch, desalting with nanofiltration membrane to remove monovalent ions and reduce salt content to obtain rhizoma Gastrodiae extractive solution, concentrating and drying to obtain rhizoma Gastrodiae extract. And measuring gastrodin.
Example 6:
(1) Preparing rhizoma Gastrodiae hydrolysate: the fresh gastrodia elata is taken as a raw material, cleaned, pulped by a pulping machine, and the fresh gastrodia elata is prepared according to the mass of the fresh gastrodia elata: purified water was added in a ratio of water=1:6 (m: v), followed by milling with a colloid mill; obtaining gastrodia elata pulp; adding sodium hydroxide solution into rhizoma Gastrodiae slurry, adjusting pH to 12, heating to 80deg.C, maintaining for 200 min for constant temperature hydrolysis, and adjusting pH to neutrality to obtain hydrolyzed primary slurry; treating hydrolyzed pulp by conventional filtration method to remove precipitate and particulates, treating filtrate with ultrafiltration membrane to remove macromolecular substances such as protein and starch, desalting with nanofiltration membrane to remove monovalent ions and reduce salt content to obtain rhizoma Gastrodiae hydrolysate, concentrating and drying to obtain rhizoma Gastrodiae hydrolysate. And measuring gastrodin.
(2) Preparation of a non-hydrolytic control: the fresh gastrodia elata is taken as a raw material, cleaned, pulped by a pulping machine, and the fresh gastrodia elata is prepared according to the mass of the fresh gastrodia elata: adding purified water in a ratio of water=1:6 (m: v), and grinding with colloid mill to obtain rhizoma Gastrodiae pulp; directly heating rhizoma Gastrodiae slurry to 80deg.C without adding alkali solution, and maintaining for 200 min; treating the heat-preserved rhizoma Gastrodiae slurry by conventional filtration method to remove precipitate and particulates, treating the filtrate with ultrafiltration membrane to remove macromolecular substances such as protein and starch, desalting with nanofiltration membrane to remove monovalent ions and reduce salt content to obtain rhizoma Gastrodiae extractive solution, concentrating and drying to obtain rhizoma Gastrodiae extract. And measuring gastrodin.
The results of measuring the gastrodin content of the extracts obtained in examples 1 to 6 are shown in Table 1.
TABLE 1 Gastrodin content in hydrolysates of different examples and non-hydrolytic control extracts
As can be seen from the table, the gastrodia elata hydrolysate prepared by the preparation method has the advantage that compared with a control sample without hydrolysis, the content of the cannabinoid in the gastrodia elata hydrolysate prepared finally by hydrolyzing the gastrodia elata pulp under the alkaline condition is remarkably improved.
Further, in examples 1 and 4, the temperature and pH were relatively low when the gastrodia elata pulp was hydrolyzed, resulting in relatively low yields of cannabinoids in the hydrolysate, but still exceeded the non-hydrolyzed control.
Finally, it is noted that the above-mentioned preferred embodiments illustrate rather than limit the invention, and that, although the invention has been described in detail with reference to the above-mentioned preferred embodiments, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the scope of the invention as defined by the appended claims.
Claims (5)
1. The preparation method of the gastrodia elata hydrolysate is characterized by comprising the following steps of:
(1) Making rhizoma Gastrodiae into rhizoma Gastrodiae slurry;
(2) Regulating pH of rhizoma Gastrodiae slurry to 8-12 with alkali solution, hydrolyzing at 30-80deg.C for 30-200 min to obtain rhizoma Gastrodiae hydrolysate;
(3) Filtering the rhizoma Gastrodiae hydrolysate, purifying, desalting, concentrating, and drying to obtain rhizoma Gastrodiae hydrolysate.
2. The method for preparing the gastrodia elata hydrolysate according to claim 1, wherein the step (1) of preparing the gastrodia elata into gastrodia elata pulp comprises the following specific steps: taking dry gastrodia elata as a raw material, crushing the dry gastrodia elata into gastrodia elata powder, and mixing the gastrodia elata powder with the following raw materials in mass: purified water is added in the proportion of (10-20) (m: v) water=1, and then the gastrodia elata pulp is obtained by colloid milling.
3. The method for preparing the gastrodia elata hydrolysate according to claim 1, wherein the step (1) of preparing the gastrodia elata into gastrodia elata pulp comprises the following specific steps: the method comprises the steps of taking fresh gastrodia elata as a raw material, cleaning the fresh gastrodia elata, pulping by a pulping machine, and obtaining the gastrodia elata according to the mass: purified water is added in the proportion of (4-8) (m: v) water=1, and then the gastrodia elata pulp is obtained by colloid milling.
4. The method for preparing a gastrodia elata hydrolysate according to claim 1, characterized in that the pH of gastrodia elata pulp is adjusted to 11.5-12 by alkali liquor in the step (2).
5. The method for preparing a gastrodia elata hydrolysate according to claim 1, wherein the temperature in the step (2) is controlled to be 50-60 ℃.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101169355A (en) * | 2007-11-28 | 2008-04-30 | 四川省中医药科学院 | Total gastrodine and bound gastrodine detection method |
| CN109601805A (en) * | 2018-12-19 | 2019-04-12 | 遵义茶溶天下生物科技有限公司 | A kind of preparation method for the gastrodia elata submicron powder that boiling water homogenate is fixed |
| CN111000944A (en) * | 2019-12-23 | 2020-04-14 | 云南中医药大学 | Gastrodia elata extract and preparation method and application thereof |
-
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101169355A (en) * | 2007-11-28 | 2008-04-30 | 四川省中医药科学院 | Total gastrodine and bound gastrodine detection method |
| CN109601805A (en) * | 2018-12-19 | 2019-04-12 | 遵义茶溶天下生物科技有限公司 | A kind of preparation method for the gastrodia elata submicron powder that boiling water homogenate is fixed |
| CN111000944A (en) * | 2019-12-23 | 2020-04-14 | 云南中医药大学 | Gastrodia elata extract and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 方伟 等: "中药制药工程原理与设备", vol. 1, 西安交通大学出版社, pages: 18 - 19 * |
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