CN116458939A - Visual micro-tissue sampling method for endometrium - Google Patents
Visual micro-tissue sampling method for endometrium Download PDFInfo
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- CN116458939A CN116458939A CN202310248591.3A CN202310248591A CN116458939A CN 116458939 A CN116458939 A CN 116458939A CN 202310248591 A CN202310248591 A CN 202310248591A CN 116458939 A CN116458939 A CN 116458939A
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- 238000005070 sampling Methods 0.000 title claims abstract description 68
- 238000000034 method Methods 0.000 title claims abstract description 32
- 210000004696 endometrium Anatomy 0.000 title claims abstract description 31
- 230000000007 visual effect Effects 0.000 title claims abstract description 21
- 239000000523 sample Substances 0.000 claims abstract description 32
- 230000003068 static effect Effects 0.000 claims abstract description 13
- 238000010827 pathological analysis Methods 0.000 claims abstract description 6
- 238000010008 shearing Methods 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 4
- 239000003761 preservation solution Substances 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 239000002184 metal Substances 0.000 claims description 3
- 230000036285 pathological change Effects 0.000 claims description 3
- 231100000915 pathological change Toxicity 0.000 claims description 3
- 239000004033 plastic Substances 0.000 claims description 3
- 239000000741 silica gel Substances 0.000 claims description 3
- 229910002027 silica gel Inorganic materials 0.000 claims description 3
- 230000000694 effects Effects 0.000 claims description 2
- 210000000056 organ Anatomy 0.000 claims 1
- 230000002357 endometrial effect Effects 0.000 abstract description 11
- 230000003902 lesion Effects 0.000 description 8
- 206010014733 Endometrial cancer Diseases 0.000 description 6
- 206010014759 Endometrial neoplasm Diseases 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 4
- 238000003745 diagnosis Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000001575 pathological effect Effects 0.000 description 3
- 206010008342 Cervix carcinoma Diseases 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 2
- 210000003484 anatomy Anatomy 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 201000010881 cervical cancer Diseases 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 230000036407 pain Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 210000004291 uterus Anatomy 0.000 description 2
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 208000009849 Female Genital Neoplasms Diseases 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 230000001680 brushing effect Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000010339 dilation Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000028149 female reproductive system neoplasm Diseases 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/02—Instruments for taking cell samples or for biopsy
- A61B10/04—Endoscopic instruments
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/04—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor combined with photographic or television appliances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/303—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor for the vagina, i.e. vaginoscopes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/02—Instruments for taking cell samples or for biopsy
- A61B10/0233—Pointed or sharp biopsy instruments
- A61B10/0266—Pointed or sharp biopsy instruments means for severing sample
- A61B10/0275—Pointed or sharp biopsy instruments means for severing sample with sample notch, e.g. on the side of inner stylet
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/02—Instruments for taking cell samples or for biopsy
- A61B10/0291—Instruments for taking cell samples or for biopsy for uterus
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Surgery (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Heart & Thoracic Surgery (AREA)
- Medical Informatics (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Radiology & Medical Imaging (AREA)
- Gynecology & Obstetrics (AREA)
- Reproductive Health (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Optics & Photonics (AREA)
- Surgical Instruments (AREA)
Abstract
The invention provides a visual micro-tissue sampling method for endometrium, which comprises a visual micro-tissue sampler and a micro-tissue sampling method. The visual micro-tissue sampler consists of a sampling shear and an endoscope, wherein the sampling shear mainly consists of a movable blade, a static blade, a spring and a driving rod; the endoscope consists of a probe, a guide shaft, a handle and a display screen, and images detected by the probe can be observed on the display screen. The micro-tissue sampling scissors are sleeved and fixed on the outer wall of the endoscope probe. The micro-tissue sampler can shear endometrial micro-tissue specimens under the visual condition for pathological diagnosis. The method provides a novel safe and effective technical method for minimally invasive sampling of the endometrium.
Description
Technical Field
The invention belongs to the technical field of medical appliances, and particularly relates to a visual micro-tissue sampling method for endometrium.
Background
Endometrial cancer is one of the common gynecological tumors, the incidence rate of the endometrial cancer is increased year by year, and along with the effective application of cytology and molecular technology in cervical cancer diagnosis, the incidence rate of the endometrial cancer exceeds that of cervical cancer, and the endometrial cancer becomes a malignant tumor of which the incidence rate is only inferior to that of breast cancer in female reproductive system tumors. At present, the clinically common methods for pathological diagnosis and sampling of endometrium include diagnostic uterine curettage, endometrium biopsy and the like, the methods adopt a cervical canal dilation scheme, a large instrument is used for obtaining a diagnostic tissue sample, the operation is complex, the trauma to a patient is large, the discomfort and pain of the patient are obvious, and most of patients can take samples under the anesthesia condition. With the clinical application of the new technology developed by us, including the patent applied by us in the earlier stage, a cell mass preparation method (patent number: CN 201510441787X) and an automatic cell mass preparation machine and method (patent number: CN 2021102221511), the new technology can effectively treat and prepare micro-tissue slices obtained clinically, and creates conditions for clearly diagnosing or screening endometrial lesions by micro-tissue obtained by micro-trauma. At present, endometrial micro-tissue collection brushes are also started to appear on the market, and the devices are used for blindly brushing partial areas of endometrium, and due to the fact that the uterus has special structures including small cervical canal, enlarged uterine cavity and individual difference of uterine anatomy structures, micro-tissue samples of high-incidence parts of endometrial cancer are difficult to obtain, samples are not representative, and various problems of insufficient material taking, small tissue quantity and the like exist, so that pathological diagnosis results are different from clinical symptoms, other biochemical indexes and imaging examination results.
In clinical practice, there is a need to develop a minimally invasive, simple, safe and effective endometrial micro-tissue sampler and method, so as to clearly diagnose or screen endometrial lesions, reduce the sub-morbidity thereof and improve the effectiveness of clinical treatment.
Disclosure of Invention
A visual micro-tissue sampling method for endometrium comprises a visual micro-tissue sampler and a micro-tissue sampling method;
the visual micro-tissue sampler consists of a sampling shear and an endoscope; the sampling scissors are composed of a movable blade, a static blade, a spring and a driving rod, wherein the movable blade and the static blade are provided with scissor openings with similar shapes; the endoscope consists of a probe, a guide shaft, a handle and a display screen, and can observe images detected by the probe on the display screen; the sampling scissors are nested and fixed on the outer wall of the endoscope probe;
the sampling scissors can be made of hard materials such as metal, plastic, silica gel and the like; the shape of the cutting opening of the sampling shear is preferably round and square, the spring mounting position of the sampling shear can be the proximal end of the movable blade or the distal end of the movable blade, and the movable blade can be driven to repeatedly move for a plurality of times relative to the static blade to shear the tissue sample through the synergistic action of the potential force and the external force of the elastic component on the sampling shear; the movable blade of the sampling shear moves longitudinally and rotationally relative to the static blade, so that the aim of shearing tissues can be fulfilled.
After the micro-tissue sampler is stretched into the uterine cavity through the cervical canal, the endometrium is observed through the probe, the endometrium tissue is closely cut and pasted in the target area by using the sampling, the endometrium tissue is sunk into a cut, meanwhile, a driving rod is used for driving a movable blade to move relative to a static blade, and the endometrial micro-tissue is cut to obtain a diagnosis sample.
Compared with the prior art, the invention provides a novel endometrium visible micro-tissue sampling method, which has the following beneficial technical effects:
(1) The technology is used for sampling the micro-tissue sample of the endometrium under the visual condition, and can effectively solve the practical problems of difficult sampling such as small cervical canal, enlarged uterine cavity, large difference of individual anatomy structures of uterus and the like.
(2) The sampling under the visual condition can accurately sample the endometrium lesion area which is visible to the naked eye, can simply take the lesion tissue, and is accurate in sampling.
(3) Can accurately sample the high-incidence part of endometrial cancer, such as histological samples of the parts of uterine horns, uterine bottoms and the like.
(4) The endometrial micro-tissue sampler belongs to a minimally invasive method for sampling, and a patient does not need to dilate a cervical canal, so that discomfort and pain in the sampling process are greatly reduced.
(5) The micro-tissue specimens obtained by the method have sufficient quantity, and the abundance of pathological tissues (such as pathological tissues) is high, so that pathological diagnosis is facilitated.
(6) The method can simultaneously obtain the macroscopic image of the endometrium and the microscopic morphology of the tissue, and a doctor of a pathology department can refer to the macroscopic image of the endometrium when reading a slice (pathological tissue section), thereby being beneficial to making accurate pathological diagnosis on diseases.
(7) The purpose of obtaining different types of diagnosis samples can be achieved by changing the structure of the sampling scissors and the size of the scissors opening.
(8) The technical method belongs to minimally invasive sampling, is safe and effective, and can not cause damage to patients.
Drawings
FIG. 1 is a schematic diagram of the structure of an endometrial visible micro-tissue sampler.
FIG. 2 is a schematic three-dimensional structure of the sampling scissors.
Fig. 3 is a schematic diagram of the structure of the endometrial visible micro-tissue sampler in use.
Description of the embodiments
For the purposes of making the objects, technical solutions and advantages of the present invention more clearly apparent, it should be noted that the directions or positional relationships indicated by the terms "upper", "lower", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, and are merely for convenience of description and simplification of description, and do not mean or imply that the apparatus or element in question must have a specific direction, and that "connected", "mounted", etc. should be construed broadly.
As shown in fig. 1, a structural schematic diagram of an endometrium visible micro-tissue sampler, wherein the visible micro-tissue sampler is composed of a sampling shear 1 and an endoscope 2, the sampling shear 1 is composed of a movable blade 11, a static blade 12, a spring 14 and a driving rod 15, and the movable blade 11 and the static blade 12 are provided with a shearing opening 13 with similar shapes; the endoscope 2 is composed of a probe 21, a guide shaft 22, a handle 23, and a display screen 26, wherein a joystick 24 is mounted on the handle 23, and images detected by the probe 21 can be observed on the displays 26. The sampling scissors 1 are telescopically fixed on the outer wall of the probe 21 of the endoscope 2. The sampling scissors 1 can be made of hard materials such as metal, plastic, silica gel and the like, the shape of the scissors mouth 23 is preferably round and square, and the mounting position of the spring 14 of the sampling scissors 1 can be the proximal end of the movable blade 21 or the distal end of the movable blade 21.
As shown in fig. 2, a three-dimensional structure of the sampling shear 1 is schematically shown, and key structures of the sampling shear 1 are a movable blade 11, a static blade 12, a shear opening 13 and a spring 14.
As shown in fig. 3, the visual micro-tissue sampler of endometrium is shown in a schematic structure in use, when in use, the finger ring 16 is pulled by fingers, the driving rod 15 and the movable blade 11 are pulled, the movable blade 11 is displaced relative to the static blade 12, and the spring 14 is compressed at the same time, so as to shear the tissue sunk in the shear port 13; then the finger ring 16 is released and the spring 14 drives the movable blade 11 forward, the cutting opening 13 is opened, and the tissue is ready to be cut again. The whole process is implemented under the visual condition through the endoscope 2, the pathological change area of the endometrium or the high-rise part of the tumor is observed through the probe 21 of the endoscope 2, the image detected through the probe 21 is observed on the display screen 26, the pathological change area is judged according to the image, and the sampling scissors 1 are moved to a new target area through the handle 23 and the operating lever 24 on the handle.
Specific embodiments: (1) The handle 23 of the endoscope is held, the probe 21 and the sampling scissors 1 which are telescopically fixed outside the probe 21 are driven by the guide shaft 22, and the sampling scissors extend into the uterine cavity by the cervical canal.
(2) The morphological structure of the endometrial surface is detected by the probe 21, and the image of the endometrial surface detected by the probe 21 is viewed on the display screen 26, and the possible lesion area is viewed.
(3) After the lesion area is found, the positions of the probe 21 and the sampling scissors 1 are moved by operating the handle 23 and the operating lever 24 on the handle, the sampling scissors 1 are attached to the surface of the tissue in the lesion area, and the sampling scissors 1 are slightly pressed, so that the tissue is sunk into the scissors opening 13 of the sampling scissors.
(4) By pulling back the finger ring 16 with the finger, the movable blade 11 of the sampling scissors 1 is pulled back by the driving rod 15, the movable blade 11 is driven to displace relative to the stationary blade 12, the spring 14 is compressed, and the tissue is cut by the shearing action of the movable blade 11 and the stationary blade 12.
(5) The finger ring 16 is loosened, the spring 14 is stretched, the movable blade 11 is driven to move by the spring 14, the shearing opening 13 of the sampling shear 1 is opened, and the micro-tissue to be sheared at the next position is prepared.
(6) When no obvious lesion is observed, the handle 23 and the control rod 24 thereon are used for driving the probe 21 and the sampling scissors 1 to move to a tumor high-hair position, such as the uterine horn, the uterine fundus and other parts for cutting micro tissues.
(7) After the sampling is completed, the finger ring 16 and the driving rod 15 connected with the finger ring are pulled by fingers, the movable blade 11 is driven to move backwards, the shearing opening 13 is closed, and the whole sampler is pulled to be taken out.
(8) The sampling scissors 1 are stretched into the preservation solution of the tissue preservation bottle, and the micro-tissues on the sampling scissors 1 are eluted into the tissue preservation solution for inspection.
Claims (9)
1. A visual micro-tissue sampling method for endometrium comprises a visual micro-tissue sampler and a micro-tissue sampling method; the visual micro-tissue sampler consists of a sampling shear and an endoscope;
the sampling scissors consist of a movable blade, a static blade, a spring and a driving rod, wherein the movable blade and the static blade are provided with scissor openings with similar shapes;
the endoscope consists of a probe, a guide shaft, a handle and a display screen, and images detected by the probe can be observed on the display screen;
the sampling scissors are nested and fixed on the outer wall of the endoscope probe;
the micro-tissue sampling method is a method for performing pathological diagnosis by shearing a micro-tissue sample from an endometrium pathological change part under a visible condition by using the micro-tissue sampler.
2. The method for sampling visual micro-tissue of endometrium according to claim 1, wherein the sampling scissors are made of hard materials such as metal, plastic, silica gel, etc.
3. The method of claim 1, wherein the force and the external force of the elastic member on the sampling scissors cooperate to drive the movable blade to repeatedly move relative to the stationary blade for shearing the tissue sample a plurality of times.
4. The method of taking a sample of visible micro-tissue of an endometrium as set forth in claim 1, wherein the moving blade of the sampling scissors is moved longitudinally and rotationally relative to the stationary blade to effect the shearing of the tissue.
5. The method of claim 1, wherein the spring-mounted position of the sampling scissors is either proximal or distal to the movable blade.
6. A method of visual micro-tissue sampling of the endometrium according to claim 1, wherein the shape of the incision of the sampling scissors is preferably circular and square.
7. The method for visual micro-tissue sampling of endometrium according to claim 1, wherein after the micro-tissue sampler is inserted into the uterine cavity through the cervical canal, the endometrium is observed through the probe, the sampling is clipped to the endometrium tissue in the target area, and the driving rod is used for driving the movable blade to move relative to the static blade, so that the micro-tissue of the endometrium is clipped to obtain the diagnostic sample.
8. The method of claim 1, wherein the sampled tissue is sheared in a tissue preservation solution, and the tissue is eluted into the preservation solution for examination.
9. A method of visual micro-tissue sampling of the endometrium according to claim 1, wherein the tissue sampler is also used as a sampling device for organs in small spaces other than the endometrium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310248591.3A CN116458939A (en) | 2023-03-15 | 2023-03-15 | Visual micro-tissue sampling method for endometrium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310248591.3A CN116458939A (en) | 2023-03-15 | 2023-03-15 | Visual micro-tissue sampling method for endometrium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116458939A true CN116458939A (en) | 2023-07-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310248591.3A Pending CN116458939A (en) | 2023-03-15 | 2023-03-15 | Visual micro-tissue sampling method for endometrium |
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| Country | Link |
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| CN (1) | CN116458939A (en) |
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2023
- 2023-03-15 CN CN202310248591.3A patent/CN116458939A/en active Pending
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