CN116444315A - Microalgae biological fertilizer for improving yield of capsicum, accelerating color conversion and improving color value and method - Google Patents
Microalgae biological fertilizer for improving yield of capsicum, accelerating color conversion and improving color value and method Download PDFInfo
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- 235000002566 Capsicum Nutrition 0.000 title claims abstract description 51
- 239000003337 fertilizer Substances 0.000 title claims abstract description 50
- 239000001390 capsicum minimum Substances 0.000 title claims abstract description 18
- 238000006243 chemical reaction Methods 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims abstract description 11
- 240000008574 Capsicum frutescens Species 0.000 title claims abstract 3
- 241000758706 Piperaceae Species 0.000 claims abstract description 28
- 241000192542 Anabaena Species 0.000 claims abstract description 26
- 241000195663 Scenedesmus Species 0.000 claims abstract description 26
- 230000012010 growth Effects 0.000 claims abstract description 24
- 230000001737 promoting effect Effects 0.000 claims abstract description 13
- 239000001963 growth medium Substances 0.000 claims abstract description 12
- 238000009630 liquid culture Methods 0.000 claims abstract description 11
- 238000012258 culturing Methods 0.000 claims abstract description 4
- 238000002360 preparation method Methods 0.000 claims abstract description 3
- 230000001965 increasing effect Effects 0.000 claims description 17
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 10
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 claims description 5
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 5
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 5
- 239000001099 ammonium carbonate Substances 0.000 claims description 5
- 235000012501 ammonium carbonate Nutrition 0.000 claims description 5
- 239000001110 calcium chloride Substances 0.000 claims description 5
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 5
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 5
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 5
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 5
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims description 5
- 229910052939 potassium sulfate Inorganic materials 0.000 claims description 5
- 235000011151 potassium sulphates Nutrition 0.000 claims description 5
- 239000001632 sodium acetate Substances 0.000 claims description 5
- 235000017281 sodium acetate Nutrition 0.000 claims description 5
- 239000003621 irrigation water Substances 0.000 claims description 3
- 239000002609 medium Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 22
- 239000002689 soil Substances 0.000 abstract description 16
- 230000000694 effects Effects 0.000 abstract description 12
- 239000000126 substance Substances 0.000 abstract description 12
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 11
- 238000012546 transfer Methods 0.000 abstract description 5
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 4
- 239000011574 phosphorus Substances 0.000 abstract description 4
- 229910052698 phosphorus Inorganic materials 0.000 abstract description 4
- 244000005700 microbiome Species 0.000 abstract description 3
- 235000021049 nutrient content Nutrition 0.000 abstract description 3
- 241000208293 Capsicum Species 0.000 description 15
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 12
- 235000013399 edible fruits Nutrition 0.000 description 11
- 241000196324 Embryophyta Species 0.000 description 10
- 229930002868 chlorophyll a Natural products 0.000 description 8
- 229930002869 chlorophyll b Natural products 0.000 description 8
- NSMUHPMZFPKNMZ-VBYMZDBQSA-M chlorophyll b Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C=O)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 NSMUHPMZFPKNMZ-VBYMZDBQSA-M 0.000 description 8
- 241000722363 Piper Species 0.000 description 7
- 235000015097 nutrients Nutrition 0.000 description 6
- 239000006002 Pepper Substances 0.000 description 5
- 235000016761 Piper aduncum Nutrition 0.000 description 5
- 235000017804 Piper guineense Nutrition 0.000 description 5
- 235000008184 Piper nigrum Nutrition 0.000 description 5
- 229930002875 chlorophyll Natural products 0.000 description 4
- 235000019804 chlorophyll Nutrition 0.000 description 4
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 4
- 241000195493 Cryptophyta Species 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- 230000000975 bioactive effect Effects 0.000 description 2
- 239000000306 component Substances 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241001136280 Chlamydomonas mexicana Species 0.000 description 1
- 244000249214 Chlorella pyrenoidosa Species 0.000 description 1
- 235000007091 Chlorella pyrenoidosa Nutrition 0.000 description 1
- 240000009108 Chlorella vulgaris Species 0.000 description 1
- 235000007089 Chlorella vulgaris Nutrition 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000008358 core component Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000003334 potential effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
Abstract
Microalgae biofertilizer and method for improving yield, color conversion and color value of capsicum comprise nitrogen fixation anabaena and scenedesmus yacyb 232 and liquid culture medium for promoting growth of microalgae; the preparation method comprises preparing liquid culture medium for promoting microalgae growth, culturing in BG11 culture medium, collecting nitrogen-fixing anabaena and Scenario Szechwan Yaccyb232, and mixing the two. The invention can promote the growth of the peppers, improve the yield of the peppers, promote the color transfer of the peppers, improve the quality of the peppers and the color value of the peppers, has the effect of dissolving phosphorus, can increase the nutrient content of soil, promote the growth of beneficial microorganisms in the soil, reduce the application amount of chemical fertilizers and is environment-friendly.
Description
Technical Field
The invention relates to the technical field of microalgae fertilizers, in particular to a microalgae biological fertilizer and a method capable of improving the yield of peppers, accelerating color conversion and improving color value.
Background
The continuous application of the inorganic fertilizer can lead to the reduction of the pH value and the organic matter content of the soil, and the problems of soil salinization, soil hardening, soil acidification and the like are generated, so that the plant health is adversely affected. The traditional fertilizer application habit is preferential to the application of chemical fertilizers, especially the application of nitrogen fertilizers, so that elements such as phosphorus, potassium, calcium and the like with large demand on pepper plants are easily lacked, normal growth and development of the plants are influenced, and the yield of the peppers is seriously influenced. The common organic compound fertilizer has a wide application range, so that the content of various nutrient elements is uniform, the proportion of the nutrient elements is possibly unsuitable for pepper growth, and the problems of low pepper yield, slow fruit color transfer and the like are caused. Thus, there is an urgent need in agriculture to find an environmentally friendly alternative that can both maintain higher agricultural productivity and promote environmental regeneration to reduce the application of inorganic fertilizers.
Microalgae are again in the field of view of people as a potential sustainable alternative to protecting and enhancing crops. The microalgae biofertilizer can promote the conversion of substances in soil, improve the effectiveness of nutrients, increase the fertility of the soil and improve the physicochemical properties of the soil through the vital activities of microalgae, and can also influence microorganisms in the soil. However, the effects of different microalgae are different, and the content of the secreted bioactive substances of a single algae cell is low.
At present, no research on using microalgae as a core component of a biological fertilizer to improve the yield of the capsicum and accelerate the color conversion of the capsicum is available. Therefore, it is necessary to invent a microalgae bio-fertilizer capable of improving the yield of the capsicum and accelerating the color conversion thereof.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides the microalgae biological fertilizer and the method for improving the yield of the peppers, accelerating color conversion and improving the color value, which can promote the growth of the peppers, improve the yield of the peppers and accelerate the color conversion of fruits while increasing the content of available nutrients in soil.
The microalgae biological fertilizer for improving the yield of the peppers, accelerating color conversion and improving color value is characterized by comprising the following components: the nitrogen-fixing anabaena and scenedesmus YACCYB232 and the liquid culture medium for promoting the growth of microalgae comprise the nitrogen-fixing anabaena and scenedesmus YACCYB232 in a proportion of 2:1-1:2, the concentration of the nitrogen-fixing anabaena is 0.6-2 g/L, and the concentration of the scenedesmus YACCYB232 or the chlorella pyrenoidosa is 0.6-2 g/L.
The liquid culture medium for promoting the growth of the microalgae comprises the following components in per liter of solution: 1-2g/L of monopotassium phosphate, 1-1.2g/L of potassium sulfate, 0.45-0.5g/L of calcium chloride, 0.3-0.35g/L of magnesium sulfate, 0.1-0.12g/L of ammonium carbonate and 0.07-0.08g/L of sodium acetate.
The microalgae biological fertilizer for improving the yield of the peppers, accelerating color conversion and improving color value has the total nitrogen content of more than or equal to 384 mg/L, the total phosphorus content of more than or equal to 227 mg/L and the total potassium content of more than or equal to 449 mg/L.
The preparation method of the microalgae biological fertilizer for improving the yield of the peppers, accelerating color conversion and improving color value is characterized by comprising the following steps of:
(1) 1-2g of monopotassium phosphate, 1-1.2g of potassium sulfate, 0.3-0.35g of magnesium sulfate, 0.45-0.5g of calcium chloride, 0.07-0.08g of sodium acetate and 0.1-0.12g of ammonium carbonate are dissolved in 1L of water to obtain a liquid culture medium for promoting microalgae growth;
(2) Culturing and collecting ratio in BG11 medium is 2:1-1:2 and scenedesmus azotemlobus YACCYB232;
(3) Mixing the microalgae collected in the step 2 with the liquid culture medium for promoting the growth of the microalgae obtained in the step 1 to obtain the required microalgae biological fertilizer, wherein the concentration of the nitrogen-fixing anabaena and the scenedesmus YACCYB232 is 0.6-2 g/L.
The application method of the microalgae biological fertilizer prepared by the method is characterized by comprising the following steps of:
the application amount of the obtained microalgae biological fertilizer is 200-500 mL/mu, and the obtained microalgae biological fertilizer is diluted by irrigation water and used once in 2 weeks;
the invention is mainly used in the technical field of microalgae fertilizers, however, the functions of different microalgae are different, and the content of the bioactive substances secreted by a single algae cell is low, so that the combination of two or more algae with different potential properties is an effective strategy for improving the activity of the microalgae fertilizer. In addition, microalgae combination is performed according to the characteristics of crops and the growth characteristics, so that the effect of microalgae secretion products on plant growth promotion is realized.
The microalgae screened by the invention can release substances which promote the growth of the peppers, improve the yield of the peppers and promote the color conversion of the peppers in the growth process, and has the effect of dissolving phosphorus. In addition, the nitrogen-fixing anabaena contained in the microalgae bio-fertilizer can convert nitrogen in the air into a bioavailable N-containing substance through the effect of nitrogen fixation. The various nutrients contained in the fertilizer can also increase the nutrient content in the soil and reduce the use amount of the fertilizer. At the same time, photosynthesis is beneficial to increasing the oxygen content in the soil.
Therefore, the invention has the following beneficial effects: (1) The nutrient content of the soil is increased, and the growth of beneficial microorganisms in the soil is promoted; (2) reducing the amount of fertilizer used; (3) promoting the growth of capsicum; (4) increasing the yield of capsicum; (5) promoting the color conversion of the capsicum and improving the color value and quality; (6) no environmental pollution.
Drawings
FIG. 1 shows the pepper plants treated at the end of the experiment in the examples.
Fig. 2 shows the pepper fruits treated in each example.
FIG. 3 shows the heights of the treated plants in the examples.
FIG. 4 shows the treated stems in the examples.
FIG. 5 shows the length of each treated leaf in the examples.
FIG. 6 is a view showing the width of each processing blade in the example.
FIG. 7 shows root system activity of each treated plant in the examples.
FIG. 8 shows the process throughput of the examples.
Detailed Description
The invention is further illustrated by the following examples and figures.
In the embodiment, the microalgae bio-fertilizer containing different microalgae is prepared by taking Chinese ocean university as an experimental place and growing red dragon 23 peppers as experimental objects through the following steps:
(1) 1-2g of monopotassium phosphate, 1-1.2g of potassium sulfate, 0.3-0.35g of magnesium sulfate, 0.45-0.5g of calcium chloride, 0.07-0.08g of sodium acetate and 0.1-0.12g of ammonium carbonate are dissolved in 1L of water to obtain a liquid culture medium for promoting microalgae growth;
(2) Culturing and collecting nitrogen-fixing anabaena, scenedesmus yacyb 232, scenedesmus, chlamydomonas mexicana, chlorella vulgaris and small Shan Qizao in BG11 medium;
(3) And (3) mixing the microalgae collected in the step (2) with the solution A obtained in the step (1) respectively to obtain the microalgae biological fertilizer for experiments.
The application amount of the obtained microalgae biological fertilizer is 500 mL/mu, and the obtained microalgae biological fertilizer is diluted by irrigation water and used once in 4 weeks.
The types of microalgae added in each treatment are shown in Table 1.
TABLE 1 kinds of microalgae added for each treatment
After 112 days of culture, as shown in fig. 1, the experimental group containing nitrogen-fixing anabaena and scenedesmus yacyb 232 grew best compared with the control group, the fertilizer group and the experimental group to which other microalgae were applied.
As shown in fig. 3-6, the experimental group containing anabaena azotis and scenedesmus yacybb 232 was significantly increased in plant height, stem thickness, leaf length and leaf width compared to the control group, the fertilizer group and the experimental group to which other microalgae were applied. At the end of the experiment, compared with the control group, the plant height, stem thickness, leaf length and leaf width of the experimental group containing the nitrogen fixation anabaena and the scenedesmus yacyb 232 are respectively increased by 33.87%, 22.72%, 21.43% and 30.67%, the plant height, stem thickness, leaf length and leaf width of the experimental group containing other microalgae are respectively increased by 12.42%, 10.26%, 4.46% and 16%, and the plant height, stem thickness, leaf length and leaf width of the experimental group containing other microalgae can be increased by 21.13%, 10.44%, 11.90% and 26.67%.
TABLE 2 flowering and fruiting times of peppers and color change times
As shown in Table 2, compared with the control group, the flowering and fruiting time and the color conversion time of the pepper in the chemical fertilizer group are not obviously advanced, the flowering and fruiting time of the pepper in the experimental group containing the nitrogen fixation anabaena and the scenedesmus YACCYB232 are advanced by 12-14 days, and the flowering and fruiting time of the experimental group applying other microalgae can be advanced by 9-10 days at most. The color transfer time of the capsicum in the experimental group containing the nitrogen-fixing anabaena and the scenedesmus YACCYB232 is reduced by 2-3 days.
TABLE 3 root morphology for each treatment
As can be seen from table 3 and fig. 7, at the end of the test, the total root length, total root surface area, root average diameter, root volume and root activity of the experimental group containing anabaena and scenedesmus yacyb 232 were increased by 182.35%, 424.23%, 103.07%, 907.26% and 94.07%, respectively, and the fertilizer was increased by 36.16%, 105.42%, 65.03%, 220.83% and 34.00%, respectively, and the total root length, total root surface area, root average diameter, root volume and root activity of the experimental group to which other microalgae were applied could be increased by 148.91%, 364.58%, 106.83%, 811.19% and 50.16%, respectively, as compared to the control group. From this, compared with the control group, the chemical fertilizer group and the experimental group using other microalgae, the experimental group containing the nitrogen fixation anabaena and the scenedesmus yacybb 232 has the advantages of developed root system morphology, strong root system activity and strong capability of absorbing and utilizing various nutrients from soil.
TABLE 4 chlorophyll content of the treated plants
As can be seen from table 4, at the end of the test, the chlorophyll a, chlorophyll b, chlorophyll a/b and total chlorophyll content of the experimental group containing anabaena and scenedesmus yacyb 232 were increased by 130.76%, 67.85%, 34.27% and 108.69%, respectively, and the chlorophyll a, chlorophyll b, chlorophyll a/b and total chlorophyll content of the experimental group to which other microalgae were applied were increased by 75.62%, 40.73%, 21.98% and 63.38%, respectively, and the chlorophyll a, chlorophyll b, chlorophyll a/b and total chlorophyll content of the experimental group to which other microalgae were applied could be increased by 84.71%, 66.86%, 21.63% and 76.93%, respectively, as compared with the control group. From this, compared with the control group, the fertilizer group and the experimental group to which other microalgae are applied, the values of chlorophyll a, chlorophyll b and chlorophyll a/b of the capsicum in the experimental group containing the nitrogen fixation anabaena and the scenedesmus yacyb 232 are higher, and the light absorption and utilization capability of the capsicum is stronger.
TABLE 5 fruit quality and fruit color Scale for each treatment
As shown in fig. 2 and table 5, compared with the experimental group to which the chemical fertilizer and other microalgae are applied, the experimental group containing the nitrogen fixation anabaena and the scenedesmus yacybb 232 has significantly improved fruit length, fruit width, fruit weight and individual plant number, and also significantly improved fruit quality and color value. The experimental group containing nitrogen fixation anabaena and scenedesmus has the advantages of longer and wider capsicum fruits, and the fruit importance is lower than that of the chemical fertilizer group.
As shown in fig. 8, the experimental group containing anabaena and scenedesmus yacyb 232 showed a significant increase in the yield of capsicum compared to the control group, the fertilizer group and the other experimental groups. Compared with the control group, the yield of the capsicum in the experimental group of the fertilizer group, the nitrogen-fixing anabaena and the scenedesmus YACCYB232 is respectively increased by 81 percent and 278.49 percent. The yield of the capsicum of the experimental group containing the nitrogen-fixing anabaena and the gulfweed is not obviously different from that of the chemical fertilizer group. The yield of capsicum in the experimental group to which other microalgae are applied is higher than that of the control group by 123.83% at most.
Conclusion: from the examples, it can be seen that the microalgae biological fertilizers containing different microalgae have different effects on the growth, yield and color transfer time of the peppers, and unsuitable microalgae can even be detrimental to the growth of the peppers in reducing the yield of the peppers. Wherein the microalgae biological fertilizer provided by the patent obviously promotes the growth of the peppers, improves the yield of the peppers, improves the quality of the peppers, promotes the color transfer of the peppers and improves the color value of the peppers.
Claims (4)
1. The microalgae biological fertilizer for improving the yield of the peppers, accelerating color conversion and improving color value is characterized by comprising the following components: the nitrogen-fixing anabaena and scenedesmus YACCYB232 and the liquid culture medium for promoting the growth of microalgae comprise the nitrogen-fixing anabaena and scenedesmus YACCYB232 in a proportion of 2:1 to 1:2, the concentration of the nitrogen-fixing anabaena is 0.6-2 g/L; the concentration of the scenedesmus YACCYB232 is 0.6-2 g/L.
2. Microalgae biofertilizer for increasing yield of capsicum and accelerating color conversion and increasing color value as claimed in claim 1, wherein the liquid culture medium for promoting growth of microalgae comprises the following components per liter of solution: 1-2g/L of monopotassium phosphate, 1-1.2g/L of potassium sulfate, 0.45-0.5g/L of calcium chloride, 0.3-0.35g/L of magnesium sulfate, 0.1-0.12g/L of ammonium carbonate and 0.07-0.08g/L of sodium acetate.
3. The preparation method of the microalgae biological fertilizer for improving the yield of the peppers, accelerating color conversion and improving color value is characterized by comprising the following steps of:
(1) Dissolving 1-2g of monopotassium phosphate, 1-1.2g of potassium sulfate, 0.3-0.35g of magnesium sulfate, 0.45-0.5g of calcium chloride, 0.07-0.08g of sodium acetate and 0.1-0.12g of ammonium carbonate in 1L of water to obtain a liquid culture medium for promoting microalgae growth;
(2) Culturing and collecting ratio in BG11 medium is 2:1-1:2 and scenedesmus azotemlobus YACCYB232;
(3) Mixing the microalgae collected in the step 2 with the liquid culture medium for promoting the growth of the microalgae obtained in the step 1 to obtain the required microalgae biological fertilizer, wherein the concentration of the nitrogen-fixing anabaena and the scenedesmus YACCYB232 is 0.6-2 g/L.
4. The method for using the microalgae bio-fertilizer prepared by the method of claim 3, which is characterized by comprising the following steps:
the microalgae biological fertilizer obtained in claim 3 is applied at a rate of 200-500 mL/mu, and the microalgae biological fertilizer obtained in claim 3 is diluted with irrigation water for use, and the application frequency is once every 2 weeks.
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Citations (5)
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| CN107043280A (en) * | 2017-05-22 | 2017-08-15 | 秦烽曦 | A kind of biological compound fertilizer and preparation method thereof |
| US20210171413A1 (en) * | 2018-06-21 | 2021-06-10 | Algae Innovations Netherlands B.V. | Use of green microalgae to improve plant growth |
| US20220142178A1 (en) * | 2020-11-11 | 2022-05-12 | Heliae Development, Llc | Methods of enhancing crop quality with microalgae compositions |
| CN115417708A (en) * | 2022-07-28 | 2022-12-02 | 中国海洋大学 | Water culture nutrient solution and method for promoting growth of pepper seedlings |
| WO2023109804A1 (en) * | 2021-12-14 | 2023-06-22 | 九江地福来农业科技发展有限公司 | Preparation method for algae active cell composite biofertilizer and use thereof |
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| CN107043280A (en) * | 2017-05-22 | 2017-08-15 | 秦烽曦 | A kind of biological compound fertilizer and preparation method thereof |
| US20210171413A1 (en) * | 2018-06-21 | 2021-06-10 | Algae Innovations Netherlands B.V. | Use of green microalgae to improve plant growth |
| US20220142178A1 (en) * | 2020-11-11 | 2022-05-12 | Heliae Development, Llc | Methods of enhancing crop quality with microalgae compositions |
| WO2023109804A1 (en) * | 2021-12-14 | 2023-06-22 | 九江地福来农业科技发展有限公司 | Preparation method for algae active cell composite biofertilizer and use thereof |
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