CN116440057A - Active composition based on composite plant fermentation concentrate and preparation method thereof - Google Patents
Active composition based on composite plant fermentation concentrate and preparation method thereof Download PDFInfo
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- CN116440057A CN116440057A CN202310712769.5A CN202310712769A CN116440057A CN 116440057 A CN116440057 A CN 116440057A CN 202310712769 A CN202310712769 A CN 202310712769A CN 116440057 A CN116440057 A CN 116440057A
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- plant fermentation
- lactobacillus
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Classifications
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/524—Preservatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses an active composition based on a composite plant fermentation concentrated solution and a preparation method thereof, and relates to the technical field of antibacterial materials. The adopted composite plant fermentation concentrated solution is prepared by taking a composite plant extract consisting of a kudzu root extract, a rhizoma polygonati extract, a ampelopsis grossedentata extract, a alpinia oxyphylla extract and a clove extract as raw materials through composite lactobacillus fermentation, and the active components of the multiple micromolecules are derived from the same living culture body, are compatible and have no antagonism, and can be rapidly absorbed and exert the functions. The composite plant fermentation concentrated solution not only contains micromolecular active ingredients obtained by fermenting the plant raw materials, but also contains lactobacillus fermentation lysate, and can be used in combination with silver-containing antibacterial agents for further synergistic bacteriostasis, and the prepared composition has broad-spectrum antibacterial performance, has obvious inhibition effects on staphylococcus aureus, escherichia coli and candida albicans, and can be used for antibacterial products in the daily chemical field.
Description
Technical Field
The invention belongs to the technical field of antibacterial materials, and particularly relates to an active composition based on a composite plant fermentation concentrate and a preparation method thereof.
Background
With the overuse of antibiotics, drug-resistant strains rise year by year, so that the treatment pressure for controlling drug-resistant bacteria infectious diseases is continuously increased. The traditional Chinese medicinal plants have the advantages of rich resources, wide sources, small toxic and side effects, low price, good antibacterial activity of some natural plants or extracting solutions thereof, less occurrence of drug resistance, good application prospect and important significance in screening microbial inhibitors from traditional Chinese medicines.
Journal literature (preparation of plant source preservative and bacteriostasis mechanism research, zhang Mei, etc., food research and development, volume 43, stage 1) screens out 4 kinds of plant extracts from 9 kinds of plant extracts according to bacteriostasis capacity and broad spectrum of escherichia coli, staphylococcus aureus and candida albicans, and then the plant source preservative is compounded by a chessboard dilution method, and the bacteriostasis mechanism of the plant source preservative is researched by taking escherichia coli as a test bacterium. The result shows that the 4 selected plant extracts are weeping forsythiae essential oil, fig essential oil, clove essential oil and purple perilla, and the optimal formula of the botanical preservative obtained by a chessboard dilution method is weeping forsythiae essential oil, fig essential oil, clove essential oil and purple perilla extract according to the volume ratio of 1:1: l: 1. The antibacterial mechanism of the plant source preservative is to destroy the structures of the bacterial cell morphology, the cell membrane osmotic pressure, genetic materials, proteins and the like of bacteria and change the permeability of the cell membrane.
In journal literature (research on in vitro bacteriostasis of compound rhubarb extract, yang Fengqin, etc., journal of Ningxia medicine, 12 th stage) observes the in vitro bacteriostasis of 5 extracts of compound rhubarb on bacteria and fungi, and the method comprises the steps of mixing rhubarb and clove according to a certain proportion to be used as raw material medicines, adding water, distilling to extract volatile oil (I), concentrating lower water solution to obtain (II), airing dregs, extracting, separating and concentrating by using 50%, 60% and 70% ethanol to obtain 5 extracts of 50% (III), 60% (IV) and 70% (V), and researching in vitro bacteriostasis by using a test tube double dilution method in combination with an agar plate method. Researches show that 5 extracts of the compound rheum officinale have different degrees of antibacterial effect on 3 common pathogenic bacteria and one fungus, wherein the ethanol extracts with the concentration of 70% have the strongest antibacterial effect on the 3 common pathogenic bacteria and one fungus, the minimum antibacterial concentration (MIC) of the ethanol extracts with the concentration of 0.25 per milliliter on staphylococcus aureus, escherichia coli and candida albicans is 0. 0.25 mg per milliliter, the antibacterial strength of volatile oil is medium, the water extract of rheum officinale has no antibacterial effect, and the 70% ethanol extract of the compound rheum officinale has remarkable antibacterial effect on 4 tested bacteria.
The periodical literature (preliminary study of bacteriostasis of plant ferment, yi Yuan et al, food safety journal, 12 th period) takes lactobacillus plantarum as zymophyte, 6 ferment stock solutions obtained by fermenting different plant formulas as research objects, and the bacteriostasis effect of the 6 ferment on common pathogenic bacteria staphylococcus aureus, escherichia coli and candida albicans is observed through a bacteriostasis test, so that the 6 ferment has no inhibition effect on candida albicans. Wherein the rose ferment and the old ginger ferment have no inhibition effect on escherichia coli and staphylococcus aureus. Among the 6 types of enzymes, 4 types of enzymes have certain antibacterial effect on escherichia coli and staphylococcus aureus, and the strength of the antibacterial effect on escherichia coli is as follows: the relationship between fruit and vegetable dietary fiber enzyme, awaking plant enzyme, five hundred plant enzyme and mulberry enzyme, and the bacteriostasis to staphylococcus aureus is as follows: the awaking plant ferment > fruit and vegetable dietary fiber ferment > mulberry ferment > five-white plant ferment.
Patent (CN 111297984A) provides bacteriostatic composition, which comprises raw materials of 18-22 parts of astragalus membranaceus, 13-17 parts of purslane, 13-17 parts of viola philippica, 13-17 parts of centella asiatica, 8-12 parts of perilla fruit, 8-12 parts of lindera root leaves, 3-7 parts of honeysuckle, 0.08-0.12 part of vitamin E and 1-3 parts of menthol. The antibacterial composition has good inhibition effects on bacteria such as staphylococcus aureus, escherichia coli and fungi such as candida albicans, can have good treatment effects on skin itch and the like caused by the bacteria or the fungi, has low irritation, is not easy to generate dependence, can be used for a long time, and has small side effect on infants and children.
Therefore, more related researches on bacteriostasis by using traditional Chinese medicine active components exist in the prior art, but antibacterial gel with good inhibition effect on staphylococcus aureus, escherichia coli and candida albicans is not obtained in the prior art. Therefore, it is necessary to provide an active composition based on a complex plant fermentation concentrate, which adopts the complex plant fermentation concentrate and a silver-containing bacteriostat as active components and has good inhibition effect on staphylococcus aureus, escherichia coli and candida albicans.
Disclosure of Invention
Aiming at the problems existing in the prior art, the invention provides an active composition based on composite plant fermentation concentrate and a preparation method thereof. The invention adopts composite plant fermentation concentrated solution and silver-containing bacteriostat as active components, and the adopted composite plant fermentation concentrated solution is prepared by taking composite plant extracts consisting of kudzu root extract, rhizoma polygonati extract, amur ampelopsis grossedentata extract, alpinia oxyphylla extract and clove extract as raw materials through composite lactobacillus fermentation, and the contained multi-component micromolecule active components are derived from the same living culture body, are compatible and have no antagonism, and can be absorbed and exert the functions rapidly, thereby overcoming the technical bottleneck that the traditional bacteriostasis single-component or multi-component composite cannot exert the effects synchronously and balanced. The composite plant fermentation concentrated solution contains small molecular active ingredients obtained by fermenting plant raw materials, also contains lactobacillus fermentation lysate, and can be used in combination with silver-containing antibacterial agent for further synergistic bacteriostasis, and the prepared composition has broad-spectrum antibacterial performance, has good inhibition effect on staphylococcus aureus, escherichia coli and candida albicans, and can be used as antibacterial products in the field of daily chemicals.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
in a first aspect, the invention provides an active composition based on a complex plant fermentation concentrate, comprising the following components in parts by weight: 10-70 parts of composite plant fermentation concentrated solution, 0.2-2 parts of silver-containing bacteriostat, 5-30 parts of surfactant, 1-10 parts of auxiliary material, 10-50 parts of purified water and 2-10 parts of glycerol; more preferably, the composition comprises the following components in parts by weight: 25-50 parts of composite plant fermentation concentrated solution, 0.2-1 part of silver-containing bacteriostat, 10-30 parts of surfactant, 1-5 parts of auxiliary materials, 20-40 parts of purified water and 4-8 parts of glycerol.
Preferably, the composite plant fermentation concentrated solution is prepared from radix Puerariae extract, rhizoma Polygonati extract, ampelopsis grossedentata extract, fructus Alpinae Oxyphyllae extract and flos Caryophylli extract by fermenting with composite lactobacillus to obtain secondary metabolite.
Preferably, the compound lactobacillus is a mixture of lactobacillus plantarum, lactobacillus casei and lactobacillus acidophilus, and the mass ratio of the lactobacillus plantarum to the lactobacillus casei to the lactobacillus acidophilus is 1:0.5-2:1-2.
The radix Puerariae extract is rich in radix Puerariae isoflavone such as daidzin, daidzein, puerarin, formononetin and its glucoside, and has antibacterial, antiaging, antiinflammatory, and antioxidant effects.
The rhizoma Polygonati extract is rich in rhizoma Polygonati polysaccharide, diosgenin, diaminobutyric acid, aspartic acid, homoserine, digitonin, apigenin, vitexin, isoliquiritigenin, syringaresinol, and siberian Polygonum glycoside A, and can be used for skin conditioning, antibacterial, antioxidant, antiaging, skin whitening, and preventing and treating red blood streak.
The Ampelopsis grossedentata extract is rich in dihydromyricetin, myricetin and its glucoside, gamma-aminobutyric acid, methionine, water-soluble polysaccharide, etc., can remove oxygen free radical, has certain antibacterial effect on various gram-positive and gram-negative pathogenic bacteria, saccharomycetes and mould, and can be used for resisting oxidation, aging, inflammation, dry skin and bacteria.
The extract is rich in nootkatone, protocatechuic acid, chrysin, eucalyptol, gingerol, etc., and has antibacterial, PPH scavenging, superoxide dismutase (SOD) and glutathione enzyme (GSH-Px) activities enhancing, and passive skin allergic reaction inhibiting effects, and can be used for antibacterial, antioxidant, antiaging, antiallergic, and antiinflammatory effects.
The flos Caryophylli extract is rich in kaempferol, rhamnosin, myricetin and its glycoside, eugenol, phenylacetaldehyde, eugenol, acetyl eugenol, gallic acid, etc., has low MIC for bacteria, saccharomycetes and mold, has strong antibacterial effect, and can be used for resisting oxidation, resisting aging, promoting skin permeation, resisting bacteria, resisting corrosion, regulating skin, etc.
The lactobacillus fermentation lysate is an inactivated and filtered product after lactobacillus fermentation, is rich in lactic acid, peptidoglycan, teichoic acid, protein, phospholipid, sterol, fatty acid, peptide and amino acid, nucleotide, extracellular polysaccharide, and multivitamin, and can be used for skin conditioning, anti-aging, antibacterial, anti-inflammatory, moisturizing, etc.
The kudzu root extract, the rhizoma polygonati extract, the ampelopsis grossedentata extract, the alpinia oxyphylla extract and the clove extract all have antibacterial effects, and the antibacterial effects can be comprehensively improved from multiple ways by adopting the raw materials as composite plant raw materials and integrating the performance advantages of the components. The multi-component micromolecule active ingredient is derived from the same living culture body and compatible with each other without antagonism through the fermentation of the composite lactobacillus, and can be rapidly absorbed and exert the functions of the active ingredient, thereby overcoming the technical bottleneck that the traditional antibacterial single-component or multi-component composite cannot synchronously balance and exert the functions. The composite plant fermentation concentrated solution contains not only micromolecular active ingredients obtained by fermenting the plant raw materials, but also lactobacillus fermentation lysate, and can be used for further synergistic bacteriostasis after being compounded with silver-containing antibacterial agents, and the prepared composition has broad-spectrum antibacterial performance and good inhibition effect on staphylococcus aureus, escherichia coli and candida albicans.
Preferably, the composite plant fermentation concentrate is prepared from the following raw materials in parts by weight: 80-90 parts of purified water, 4.5-8.0 parts of radix puerariae extract, 1.3-3.5 parts of rhizoma polygonati extract, 0.70-2.40 parts of ampelopsis grossedentata extract, 0.70-2.20 parts of fructus alpiniae oxyphyllae extract, 0.40-1.30 parts of clove extract and 3.5-7.5 parts of lactobacillus complex.
Preferably, the preparation process of the composite plant fermentation concentrated solution comprises the following steps: mixing radix Puerariae extract, rhizoma Polygonati extract, ampelopsis grossedentata extract, fructus Alpinae Oxyphyllae extract, flos Caryophylli extract and water, adjusting pH to 2-7, adding lactobacillus after propagation, fermenting, concentrating to relative density of 0.9-1.2 at 20deg.C, centrifuging, and sterilizing; more preferably, mixing radix Puerariae extract, rhizoma Polygonati extract, ampelopsis grossedentata extract, fructus Alpinae Oxyphyllae extract, flos Caryophylli extract and water, and adjusting pH to 2.5-5; after fermentation, concentrating to a relative density of 1.0-1.15 at 20deg.C.
Preferably, the fermentation condition is 35-39deg.C for 5-8 days, and the centrifugation condition is 3000-5000r/min for 10-30min.
Preferably, the Silver-containing antibacterial agent is selected from at least one of Jetel Silver II, procare ESI and AC1000-D, HYMAg-M.
Preferably, the surfactant is selected from one or more of poloxamer 407, poloxamer 188, tween 80, span 80, AES, AOS, AEO-7, cocodiethanolamide, cocamidopropyl betaine, and more preferably poloxamer 407.
Preferably, the purified water is selected from distilled water and/or deionized water.
Preferably, the auxiliary material is selected from the group consisting of 1, 2-hexanediol, 1, 2-pentanediol and isopentyl glycol, more preferably 1, 2-hexanediol.
On the other hand, the invention also provides a preparation method of the active composition based on the composite plant fermentation concentrated solution, which comprises the following steps:
1) Adding purified water into a stirring pot, stirring and heating to 70-95deg.C, and maintaining the temperature for 20-40min;
2) Weighing the composite plant fermentation concentrated solution and auxiliary materials, and uniformly stirring;
3) Adding the mixture obtained in the step 2) and the silver-containing bacteriostat into the stirring pot in the step 1), and stirring until the mixture is uniform;
4) Adding surfactant under slow stirring, maintaining the temperature, and soaking for 1-3 hr; stirring until the mixture is dissolved and completely transparent after the soaking is completed;
5) Slowly adding glycerol for several times under stirring, and stirring to uniformity;
6) And after the sampling inspection is qualified, filtering and discharging, sealing and standing, inspecting, filling, packaging and warehousing.
Preferably, the stirring speed in the step 1) is 10-20 rpm, the slow stirring speed in the step 4) and the stirring speed after soaking are 5-10 rpm, and the stirring speed in the step 5) is 20-30 rpm.
Compared with the prior art, the invention has the following beneficial effects:
the composite plant fermentation concentrated solution adopted by the invention is prepared by taking a composite plant extract consisting of a kudzuvine root extract, a rhizoma polygonati extract, a ampelopsis grossedentata extract, a alpinia oxyphylla extract and a clove extract as raw materials through fermentation and fermentation of composite lactobacillus, and contains a plurality of micromolecule active ingredients which are derived from the same living culture body and are compatible and unopposed, so that the composite plant fermentation concentrated solution can be rapidly absorbed and plays the functions of the same, thereby overcoming the technical bottleneck that the traditional antibacterial single-component or multi-component composite cannot synchronously balance and play the functions. The composite plant fermentation concentrated solution and the silver-containing antibacterial agent are adopted as active components, and the composite plant fermentation concentrated solution not only contains micromolecular active ingredients obtained by fermenting the plant raw materials, but also contains lactobacillus fermentation lysate, and can be used in combination with the silver-containing antibacterial agent for further synergistic bacteriostasis, so that the prepared composition has broad-spectrum antibacterial performance, has remarkable inhibition effect on staphylococcus aureus, escherichia coli and candida albicans, and can be used as a bacteriostasis product in the field of daily chemicals. 3. The composition has a remarkable antibacterial effect on staphylococcus aureus, escherichia coli and candida albicans, and the antibacterial rate of the composition can reach about 70%.
Detailed Description
It is to be noted that the raw materials used in the present invention are all common commercial products, and the sources thereof are not particularly limited.
The following raw material sources are exemplary illustrations:
radix Puerariae extract: KUDZU ROOT p.e. from the biotechnology company, inc;
rhizoma Polygonati extract: polygonatuM officinale, ext, from biotechnology, inc;
ampelopsis grossedentata extract: ampelopsis grossedentata (AMPELOPSIS GROSSEDENTATA) extract from Huzhou Pu Rui biomedical technologies Co., ltd;
an extract of alpinia oxyphylla: purchased from Hunan Langlin biological resource Co., ltd;
flos Caryophylli extract: purchased from Shaanxi pannier Biotech Co., ltd;
silver-containing antimicrobial agent: jetel Silver II, available from Haen Biotechnology, inc., germany;
and (2) a surfactant: poloxamer 407, commercially available;
lactic acid bacillus: lactobacillus plantarum NDC75017, lactobacillus plantarum 14917, lactobacillus acidophilus NCFM, major laboratory deposited strains of the northeast university dairy science education department; human lactobacillus casei NCU011056, NCU011061, national emphasis laboratory deposited strains of Nanchang university food science and technology;
pH regulator: citric acid, commercially available;
auxiliary materials: 1, 2-hexanediol, commercially available;
glycerol, deionized water: are commercially available.
Preparation example 1 preparation of composite plant fermentation concentrate
1. Preparation of a culture medium: adding 6 parts of radix puerariae extract, 2 parts of rhizoma polygonati extract, 1.6 parts of ampelopsis grossedentata extract, 1.6 parts of fructus alpiniae oxyphyllae extract, 0.8 part of clove extract and 84 parts of water into a triangular flask, regulating the pH to 3.5 by using citric acid, uniformly mixing, adding a cotton plug into a bottle opening, wrapping newspaper, sterilizing for 20min in a portable autoclave at 115 ℃, and taking out for later use.
2. And (3) strain expansion culture: under the aseptic condition, respectively picking a loop of lactobacillus plantarum, lactobacillus casei and lactobacillus acidophilus thallus by using an inoculating loop, drawing a curve in different sterilized test tubes, culturing in a horizontal test tube at 32 ℃ for 7 days, and transferring again under the same operation condition after colony growth, wherein the culture condition is the same as the above. The strain is used for experiments after 3 generations of activation process.
3. Fermentation: the selected culture temperature is 35 ℃ +/-1 ℃, sterile water is added into an activated inclined surface test tube, spores are eluted, a sterilization pipette is used for sucking spore suspension, 4% of inoculation amount (the mass ratio of lactobacillus plantarum to lactobacillus casei to lactobacillus acidophilus is 1:1.5:1.5) is transferred into a standby liquid culture medium, shake culture is carried out, the rotation speed is 120 r/min, the culture temperature is 35 ℃, and the culture fermentation is carried out for 7 days. Concentrating the fermentation broth to relative density of 1.05 at 20deg.C, centrifuging at 4000r/min for 20min, and sterilizing.
Preparation example 2 preparation of composite plant fermentation concentrate
1. Preparation of a culture medium: adding 4.5 parts of radix puerariae extract, 3 parts of rhizoma polygonati extract, 2.4 parts of ampelopsis grossedentata extract, 2.2 parts of fructus alpiniae oxyphyllae extract, 0.4 part of flos caryophylli extract and 82.5 parts of water into a triangular flask, regulating the pH to 3.5 by using citric acid, mixing uniformly, adding a cotton plug into a bottle opening, wrapping newspaper, sterilizing for 20min in a portable autoclave at 115 ℃, and taking out for later use.
2. And (3) strain expansion culture: the same as in preparation example 1.
3. Fermentation: the selected culture temperature is 35 ℃ +/-1 ℃, sterile water is added into an activated inclined surface test tube, spores are eluted, a sterilization pipette is used for sucking spore suspension, 5% of inoculation amount (the mass ratio of lactobacillus plantarum to lactobacillus casei to lactobacillus acidophilus is 1:2:2) is transferred into a standby liquid culture medium, shake cultivation is carried out, the rotating speed is 120 r/min, the culture temperature is 35 ℃, and the culture fermentation is carried out for 7 days. Concentrating the fermentation broth to relative density of 1.03 at 20deg.C, centrifuging for 18min at 4500r/min, and sterilizing.
Preparation example 3 preparation of composite plant fermentation concentrate
1. Preparation of a culture medium: adding 8 parts of radix puerariae extract, 1.3 parts of rhizoma polygonati extract, 0.7 part of ampelopsis grossedentata extract, 0.7 part of fructus alpiniae oxyphyllae extract, 1.3 parts of flos caryophylli extract and 83.5 parts of water into a triangular flask, regulating the pH to 3.5 by using citric acid, uniformly mixing, adding a cotton plug into a bottle opening, wrapping newspaper, sterilizing for 20min in a portable autoclave at 115 ℃, and taking out for later use.
2. And (3) strain expansion culture: the same as in preparation example 1.
3. Fermentation: the selected culture temperature is 35 ℃ +/-1 ℃, sterile water is added into an activated inclined surface test tube, spores are eluted, a sterilization pipette is used for sucking spore suspension, 4.5% of inoculation amount (the mass ratio of lactobacillus plantarum to lactobacillus casei to lactobacillus acidophilus is 1:1.5:2) is transferred into a standby liquid culture medium, shake culture is carried out, the rotating speed is 120 revolutions per minute, the culture temperature is 35 ℃, and the culture fermentation is carried out for 7 days. Concentrating the fermentation broth to a relative density of 1.10 at 20deg.C, centrifuging at 4000r/min for 30min, and sterilizing.
Examples 1 to 3
The active compositions of examples 1-3 were added to the composite plant fermentation concentrates of preparations 1-3, respectively, and the specific formulations of the compositions are shown in Table 1.
The preparation process comprises the following steps:
1) Adding purified water into a stirring pot, stirring at 20 rpm, heating to 80deg.C, and maintaining the temperature for 30min;
2) Weighing the composite plant fermentation concentrated solution and auxiliary materials, and uniformly stirring;
3) Adding the mixture obtained in the step 2) and the silver-containing bacteriostat into the stirring pot in the step 1), and stirring until the mixture is uniform;
4) Adding surfactant under slow stirring (10 rpm), maintaining the temperature, and soaking for 2 hr; after the soaking is completed, stirring for 10 revolutions per minute until the mixture is dissolved and completely transparent;
5) Slowly adding glycerol for three times under 30 r/min stirring, and stirring to uniformity;
6) And after the sampling inspection is qualified, filtering and discharging, sealing and standing, inspecting, filling, packaging and warehousing.
Comparative example 1
Example 1 was repeated except that the composite plant material was not fermented. Namely, 6 parts of kudzuvine root extract, 2 parts of rhizoma polygonati extract, 1.6 parts of ampelopsis grossedentata extract, 1.6 parts of alpinia oxyphylla extract, 0.8 part of clove extract and 84 parts of water are uniformly stirred, concentrated to a relative density of 1.05 at 20 ℃, and sterilized to replace the active composition to be directly used.
Comparative example 2
The procedure of example 1 was repeated except that 9 parts of the kudzu vine root extract and 3 parts of the rhizoma polygonati extract were used as the medium, and that the Ampelopsis grossedentata extract, the alpinia oxyphylla extract and the clove extract were not added.
Comparative example 3
The procedure of example 1 was repeated except that 4.8 parts of Ampelopsis grossedentata extract, 4.8 parts of Alpinia oxyphylla extract and 2.4 parts of Syzygium aromaticum extract were used as the medium, and radix Puerariae extract and rhizoma Polygonati extract were not added.
Comparative example 4
The procedure of example 1 was repeated except that 6 parts of pueraria extract, 2 parts of polygonatum extract, 4 parts of ampelopsis grossedentata extract, 0 part of alpinia oxyphylla extract and 0 part of clove extract were used as the culture medium.
Comparative example 5
The procedure of example 1 was repeated except that 6 parts of pueraria extract, 2 parts of polygonatum extract, 0 part of ampelopsis grossedentata extract, 2.67 parts of alpinia oxyphylla extract and 1.33 parts of clove extract.
Comparative example 6
The procedure of example 1 was repeated except that Lactobacillus casei NCU011061 was used as the Lactobacillus casei in the fermentation step.
Comparative example 7
The procedure of example 1 was repeated except that Lactobacillus plantarum 14917 was used in the fermentation step.
Table 1 formulations of the active compositions of examples 1-3 and comparative example 1
Test examples
The antibacterial properties of examples 1 to 3 and comparative examples 1 to 7 were evaluated, and the results are shown in tables 2 to 3:
the test basis is as follows: GB 15979-2002 annex C product of sanitary Standard for Disposable sanitary products has bactericidal and bacteriostatic properties.
Testing strains: staphylococcus aureus ATCC 6538, escherichia coli 8099 and candida albicans ATCC 10231, wherein the algebra of the strains is 5 th generation.
The testing process comprises the following steps: according to the antibacterial performance test method of the dissoluble antibacterial products, the samples of examples 1-3 and comparative examples 1-9 are taken and acted for 20min, wherein the sample of example 1 has the action time of 2min, 5min, 10min and 20min, the test is repeated for 3 times, and the average value is obtained. Ambient temperature 20.3 ℃ and relative humidity 47%.
TABLE 2 antibacterial Properties (%)
As can be seen from Table 2, the active composition of example 1 acts for 20min, has an average antibacterial rate of 73.09% on staphylococcus aureus, an average antibacterial rate of 73.54% on escherichia coli, an average antibacterial rate of 69.06% on candida albicans, has a remarkable inhibitory effect on staphylococcus aureus, escherichia coli and candida albicans, and meets the requirements of GB 15979-2002 sanitary Standard for Disposable sanitary products.
TABLE 3 antibacterial Properties (%)
As can be seen from Table 3, the active compositions of examples 1-3 have significant inhibitory effects on Staphylococcus aureus, escherichia coli and Candida albicans, and meet the specifications of GB 15979-2002 hygienic Standard for Disposable sanitary products. As can be seen from the comparison of example 1 and comparative example 1, the bacteriostatic action of the system can be remarkably improved after fermentation by using the defined strain. As can be seen from comparing example 1 with comparative examples 2-5, the combination of the nootropic extract, the lilac extract and the ampelopsis grossedentata extract has a synergistic effect, while the combination of the nootropic extract, the lilac extract and the ampelopsis grossedentata extract also has a more remarkable improvement of the antibacterial effect. Comparing example 1 with comparative examples 6-7, it can be seen that the difference in strains of Lactobacillus plantarum and Lactobacillus casei of human origin has a great influence on the bacteriostatic action of the composition.
Finally, it should be noted that the above description is only for illustrating the technical solution of the present invention, and not for limiting the scope of the present invention, and that the simple modification and equivalent substitution of the technical solution of the present invention can be made by those skilled in the art without departing from the spirit and scope of the technical solution of the present invention.
Claims (10)
1. The active composition based on the composite plant fermentation concentrate is characterized by comprising the following components in parts by weight: 10-70 parts of composite plant fermentation concentrated solution, 0.2-2 parts of silver-containing bacteriostat, 5-30 parts of surfactant, 1-10 parts of auxiliary material, 10-50 parts of purified water and 2-10 parts of glycerol; wherein the composite plant fermentation concentrated solution takes radix puerariae extract, rhizoma polygonati extract, ampelopsis grossedentata extract, alpinia oxyphylla extract and clove extract as composite plant raw materials, and secondary metabolite is obtained by composite lactobacillus fermentation; the composite lactobacillus is a mixture of lactobacillus plantarum, lactobacillus casei and lactobacillus acidophilus, and the mass ratio of the lactobacillus plantarum to the lactobacillus casei to the lactobacillus acidophilus is 1:0.5-2:1-2.
2. The active composition based on a complex plant fermentation concentrate of claim 1, wherein the complex plant fermentation concentrate is prepared from the following raw materials in weight fraction: 80-90 parts of purified water, 4.5-8.0 parts of radix puerariae extract, 1.3-3.5 parts of rhizoma polygonati extract, 0.70-2.40 parts of ampelopsis grossedentata extract, 0.70-2.20 parts of fructus alpiniae oxyphyllae extract, 0.40-1.30 parts of clove extract and 3.5-7.5 parts of lactobacillus complex.
3. The active composition based on a complex plant fermentation concentrate of claim 1, wherein the complex plant fermentation concentrate is prepared by a process comprising: mixing radix Puerariae extract, rhizoma Polygonati extract, ampelopsis grossedentata extract, fructus Alpinae Oxyphyllae extract, flos Caryophylli extract and water, adjusting pH to 2-7, adding lactobacillus after propagation, fermenting, concentrating to relative density of 0.9-1.2 at 20deg.C, centrifuging, and sterilizing.
4. The active composition based on a complex plant fermentation concentrate according to claim 3, wherein the fermentation conditions are 35-39 ℃ for 5-8 days.
5. The active composition based on a complex plant fermentation concentrate according to claim 3, wherein the centrifugation conditions are 3000-5000r/min for 10-30min.
6. The active composition based on a complex plant fermentation concentrate according to claim 1, wherein the Silver-containing antimicrobial agent is selected from at least one of jepel Silver II, procare ESI, AC1000-D, HYMAg-M.
7. The active composition based on a complex plant fermentation concentrate of claim 1, wherein the surfactant is selected from one or more of poloxamer 407, poloxamer 188, tween 80, span 80, AES, AOS, AEO-7, coco diethanolamide, coco amidopropyl betaine; the purified water is selected from distilled water and/or deionized water; the auxiliary materials are selected from 1, 2-hexanediol, 1, 2-pentanediol and isopentyl glycol.
8. The active composition based on a complex plant fermentation concentrate of claim 7, wherein the surfactant is poloxamer 407 and the adjuvant is 1, 2-hexanediol.
9. A process for the preparation of an active composition based on a complex plant fermentation concentrate according to any one of claims 1 to 8, characterized in that: the method comprises the following steps:
1) Adding purified water into a stirring pot, stirring and heating to 70-95deg.C, and maintaining the temperature for 20-40min;
2) Weighing the composite plant fermentation concentrated solution and auxiliary materials, and uniformly stirring;
3) Adding the mixture obtained in the step 2) and the silver-containing bacteriostat into the stirring pot in the step 1), and stirring until the mixture is uniform;
4) Adding surfactant under slow stirring, maintaining the temperature, and soaking for 1-3 hr; stirring until the mixture is dissolved and completely transparent after the soaking is completed;
5) Slowly adding glycerol for several times under stirring, and stirring to uniformity;
6) And after the sampling inspection is qualified, filtering and discharging, sealing and standing, inspecting, filling, packaging and warehousing.
10. The method of preparing as claimed in claim 9, wherein: the stirring speed in the step 1) is 10-20 rpm, the slow stirring speed in the step 4) and the stirring speed after soaking are 5-10 rpm, and the stirring speed in the step 5) is 20-30 rpm.
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