CN116413464A

CN116413464A - Incubation unit, sample analyzer and sample analysis method

Info

Publication number: CN116413464A
Application number: CN202111672414.5A
Authority: CN
Inventors: 方泉; 练子富; 李临
Original assignee: Kemei Boyang Diagnostic Technology Shanghai Co ltd
Current assignee: Kemei Boyang Diagnostic Technology Shanghai Co ltd
Priority date: 2021-12-31
Filing date: 2021-12-31
Publication date: 2023-07-11

Abstract

The application relates to an incubation unit, a sample analyzer and a sample analysis method. The incubation unit comprises a body and a dispensing disc, wherein the dispensing disc is used for placing the reaction container and can drive the reaction container to move to different stations; the positioning assembly comprises a first driving part and a connecting clamping part, the first driving part can drive the clamping part to move, and the clamping part can be clamped with the reaction container to position the reaction container; the cup discarding assembly comprises a second driving part and a pushing part, the second driving part is used for driving the pushing part to move, and the pushing part is used for separating and pushing the reaction container from the dispensing disc to a preset position; the position detection assembly comprises at least one detection part, and the detection part is at least used for detecting the position where the pushing part pushes the reaction container to reach. According to the scheme, multiple stations are integrated into a whole through time sequence control, and the whole operation is efficient and accurate and high in reliability.

Description

Incubation unit, sample analyzer and sample analysis method

Technical Field

The application relates to the technical field of medical instruments, in particular to an incubation unit, a sample analyzer and a sample analysis method.

Background

A sample analyzer (biochemical analysis, immunoassay) is an instrument for detecting a specific substance in blood. In order to obtain a detection signal, the liquid in the cuvette is usually subjected to a specific reaction, such as a biochemical reaction, an immunological reaction. Such reactions generally need to be carried out under defined temperature conditions. If the reaction temperature does not meet the prescribed temperature, the reaction does not proceed sufficiently and a correct detection signal cannot be obtained. The incubation unit is an important component of the sample analyzer, is used for providing proper temperature for the reaction container, and needs to meet the procedures of sample injection, liquid adding, detection, unloading and the like, and for the incubation unit, the conversion and performance among the procedures have great influence on the accuracy and precision of the detection result.

Disclosure of Invention

In order to solve or partially solve the problems existing in the related art, the application provides an incubation unit, a sample analyzer and a sample analysis method, multiple stations are integrated into a whole through time sequence control, and the whole operation is efficient and accurate and has high reliability.

A first aspect of the present application provides an incubation unit for a sample analyzer, the incubation unit comprising:

the body at least comprises a cup feeding station, a sample adding and mixing station, a detection station and a cup discarding station;

the dispensing disc is movably connected with the body and used for placing the reaction container, and the action of the dispensing disc can drive the reaction container to move to different stations;

the detection station is provided with a positioning assembly, the positioning assembly comprises a first driving part and a connecting clamping part, the first driving part can drive the clamping part to move, and the clamping part can be clamped with the reaction container to position the reaction container;

the cup discarding station is provided with a cup discarding component and a position detecting component,

the cup discarding assembly comprises a second driving part and a pushing part which are matched, the second driving part is used for driving the pushing part to move, when the reaction container moves to the cup discarding station, the pushing part is used for separating and pushing the reaction container from the dispensing tray to a preset position,

The position detection assembly comprises at least one detection part, wherein the detection part is at least used for detecting the position where the pushing part pushes the reaction container to reach.

A second aspect of the present application provides a sample analyzer comprising an incubation unit, the incubation unit being as described in the foregoing.

The third aspect of the present application provides a sample analysis method, which is used for incubating a unit, the incubation unit includes a body, a dispensing tray, a positioning component, a cup discarding component and a position detection component, the dispensing tray is used for driving a reaction container placed on the dispensing tray to move among a cup feeding station, a sample adding mixing station, a detection station and a cup discarding station, the positioning component includes a first driving part and a clamping part, the cup discarding component includes a second driving part and a pushing part, the position detection component includes at least one detection part, and the sample analysis method includes:

the reaction container enters from the cup feeding station, and when the dispensing disc drives the reaction container to move to the detection station according to time sequence, the positioning assembly controls the first driving part to move so as to enable the clamping part to be clamped with the reaction container, and controls the clamping part to be clamped with the reaction container until detection is completed;

When the dispensing disc drives the reaction container to move to the cup discarding station according to time sequence, the second driving part is controlled to move so that the pushing part drives the reaction container to be separated from the dispensing disc and move to a preset position, and the detecting part is at least used for detecting the position where the pushing part pushes the reaction container to reach.

The technical scheme that this application provided can include following beneficial effect: the positioning component arranged in the incubation unit can assist in positioning the dispensing tray through the positioning component, so that the target reaction container can stop at a corresponding station when rotating along with the dispensing tray, and the position accuracy is higher. The provided cup discarding assembly is beneficial to discarding the target reaction container to a fixed position at the cup discarding station more stably and with higher precision. The position detection assembly can monitor the position of the target reaction container in the cup discarding assembly, and avoid blocking in the cup discarding assembly. Through the design in an organic whole with a plurality of stations that use in the sample analysis for incubating the unit and have higher integrated level, can conveniently quick change reaction vessel to different stations, and can maintain reaction vessel at suitable temperature all the time in the conversion process, with stability and the degree of accuracy of guaranteeing to incubate the result.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the application.

Drawings

The foregoing and other objects, features and advantages of the application will be apparent from the following more particular descriptions of exemplary embodiments of the application as illustrated in the accompanying drawings wherein like reference numbers generally represent like parts throughout the exemplary embodiments of the application.

Fig. 1 is a schematic diagram of the overall structure of an incubation unit according to an embodiment of the present application;

FIG. 2 is a schematic view of a part of an incubation unit according to the embodiment of the present application;

FIG. 3 is a schematic view of the partial structure of FIG. 2;

FIG. 4 is a schematic overall structure of a positioning assembly in an incubation unit according to an embodiment of the present disclosure;

FIG. 5 is a front view of a positioning assembly in an incubation unit shown in an embodiment of the present application;

FIG. 6 is a schematic view of the overall structure of a disposable cup assembly in an incubation unit according to an embodiment of the present disclosure;

FIG. 7 is a schematic cross-sectional view of a disposable cup assembly in an incubation unit according to the embodiments of the present application;

fig. 8 is a schematic cross-sectional view of another cup assembly in an incubation unit according to an embodiment of the disclosure.

Detailed Description

Embodiments of the present application will be described in more detail below with reference to the accompanying drawings. While embodiments of the present application are shown in the drawings, it should be understood that the present application may be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.

The terminology used in the present application is for the purpose of describing particular embodiments only and is not intended to be limiting of the present application. As used in this application and the appended claims, the singular forms "a," "an," and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It should also be understood that the term "and/or" as used herein refers to and encompasses any or all possible combinations of one or more of the associated listed items.

It should be understood that although the terms "first," "second," "third," etc. may be used herein to describe various information, these information should not be limited by these terms. These terms are only used to distinguish one type of information from another. For example, a first message may also be referred to as a second message, and similarly, a second message may also be referred to as a first message, without departing from the scope of the present application. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present application, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.

A sample analyzer (e.g., biochemical analysis, immunoassay) is an instrument for detecting a specific substance in blood. The incubation unit is an important component of the sample analyzer for providing the reaction vessel with a suitable temperature to meet the incubation requirements. In addition, in the incubation process, operations such as sample injection, sample adding and mixing, detection and unloading are required to be performed on the sample, and in the series of operation processes, the reaction container is always ensured to be at a proper temperature, so that the incubation result is prevented from being influenced. When the reaction vessel is converted to different positions in the incubation unit for corresponding operation, the conversion process is not tight, the stability is poor, the reaction vessel is not easy to maintain at a proper temperature all the time, and the final sample analysis result is easily affected under the influence of various factors.

Aiming at the problems, the embodiment of the application provides an incubation unit, and the multi-station integrated design ensures the high efficiency and stability of operation and the accuracy of detection results.

The following describes the technical scheme of the embodiments of the present application in detail with reference to the accompanying drawings.

Fig. 1 is a schematic diagram of the overall structure of an incubation unit according to an embodiment of the present application.

Fig. 2 is a schematic partial perspective view of an incubation unit according to an embodiment of the present application.

Fig. 3 is a partial schematic structure of fig. 2.

Referring to fig. 1 to 3, the incubation unit includes a main body 1, the main body 1 includes at least a cup feeding station 1a, a sample adding mixing station 1b, a detecting station 1c, and a cup discarding station 1d, a dispensing tray 6 is movably connected to the main body 1 for placing a reaction container 5, and the action of the dispensing tray 6 can drive the reaction container 5 to move to different stations. The incubation unit further comprises a positioning component 4 positioned at the detection station 1c, a cup discarding component 2 positioned at the cup discarding station 1d and a position detection component 3. The stations operated in different modes in the incubation process are integrated in the body, the reaction container 5 can be conveniently and stably converted into the corresponding station by driving the dispensing disc 6 to act, the integration level is high, and orderly and stable proceeding of each step is ensured.

In an embodiment, the body 1 includes a housing 11, an incubation tray 13, and a top cover 12, the top of the housing 11 has an open end, the top cover 12 covers the open end and is detachably connected with the housing 11 to form a containing cavity 111, the incubation tray 13 is disposed on an inner wall of the housing 11, and the dispensing tray 6 and the incubation tray 13 are located in the containing cavity 111. On the one hand, this arrangement allows the reaction vessel 5 located in the dispensing disc 6 to be always located in the receiving chamber 111 during movement, so that the reaction vessel 5 can be always maintained at a set temperature. On the other hand, the reaction vessel 5 is limited to the arrangement in the accommodating chamber 111, so that the reaction vessel 5 is prevented from jumping in the axial direction due to the influence of external factors. On the other hand, the reaction vessel 5 on the dispensing plate 6 is ensured to be in a detectable light-shielding environment.

Wherein, the top cover 12 is provided with at least one through hole 121 at a position corresponding to the sample adding and mixing station 1b, and when the reaction container 5 is located in the sample adding and mixing station 1b, liquid including but not limited to a sample, a reagent and a general liquid can be added from the through hole 121, so that the mixed liquid is obtained after the liquid is added and mixed uniformly in the reaction container 5.

Optionally, the incubation unit includes a heating component disposed in the accommodating cavity 111, for heating the accommodating cavity 111, so as to maintain the accommodating cavity 111 at a desired suitable temperature. It will be appreciated that the heating assembly may be a heating pipeline uniformly arranged on the inner wall of the incubation plate 13 or the housing 11, or other heating device capable of heating the accommodating cavity 111 to maintain the reaction vessel 5 therein at a suitable temperature, which is not particularly limited herein.

In order to ensure that the reaction vessel 5 in the accommodating chamber 111 has a stable temperature when moving to different stations, at least one side of the top cover 12 may be covered with an insulating layer, and the through hole 121 penetrates the insulating layer. The arrangement of the heat preservation layer can prevent the temperature in the accommodating cavity 111 from diffusing too fast so as to achieve the heat preservation effect. Optionally, other side walls of the housing 11 for forming the accommodating cavity 111 may also be covered with a heat insulation layer to improve the heat insulation performance of the accommodating cavity 111. For the heat preservation, the opaque material with heat preservation performance can be selected to play the light-proof effect when playing better heat preservation effect, avoid influencing sample analysis result.

Optionally, the body 1 may further comprise a shielding assembly located on the top cover 12, the shielding assembly comprising: at least one cover plate and a power part, the power part can drive the cover plate to rotate so as to drive the cover plate to cover or open the through hole 121. Through the apron that sets up, can be when detecting that reaction vessel 5 is in application of sample mixing station 1b, control power portion rotates the apron in order to open through-hole 121 to can conveniently and fast carry out the liquid feeding and mix the operation. After the completion, the power portion controls the cover plate to cover the through hole 121, so that the path of temperature diffusion in the accommodating cavity 111 is reduced, and the heat preservation effect in the accommodating cavity 111 is improved. Other mating arrangements for the shutter assembly for opening or closing one or more of the through-holes 121 are also possible and will not be described in detail herein.

In an embodiment, the cup feeding station 1a, the sample adding and mixing station 1b, the detecting station 1c and the cup discarding station 1d are circumferentially arranged on the body 1 according to a time sequence, the dispensing disc 6 is rotationally connected to the accommodating cavity 111, so that the dispensing disc 6 can rotate around an axis of the body 1, so as to drive the reaction container 5 to circumferentially rotate to the corresponding station according to a preset time sequence and start and stop after the reaction container is in place, the dispensing disc 6 is circumferentially provided with a plurality of accommodating positions 61, and the accommodating positions 61 are used for accommodating the reaction container 5. Through the form of arranging each station circumference, combine the rotation and the chronogenesis of minute injection dish 6 for the reaction vessel 5 that sets up in minute injection dish 6 can accomplish complete sample analysis work before accomplishing rotation a week, and realize the dress cup to minute injection dish 6 to reaction vessel 5 and abandon the cup. And along with the process of circumferential rotation of the preset time sequence, continuous work of a plurality of reaction containers 5 can be realized according to the entering sequence of the reaction containers 5, and the sample analysis efficiency is improved. The mode integrates each process, a plurality of containing positions 61 are circumferentially and uniformly arranged, and the reaction vessel 5 can be conveniently and rapidly rotated to the corresponding station only by controlling the rotation angle of the containing positions 61, the rotation time interval and other relevant parameters and time sequence limitation, so that the whole operation is efficient and accurate and the reliability is higher.

It will be appreciated that the incubation unit further comprises a support base 11b, the body 1 is located on the support base 11b, a support shaft rotatably connected to the support base 11b at least partially penetrates the housing 11 and the incubation plate 13 to be connected to the dispensing plate 6, and a third driving portion 11a is connected to the support shaft for driving the support shaft to rotate so as to rotate the dispensing plate 6 around the axis of the body 1. In order to realize the rotation of the dispensing disc 6, the supporting shaft can be fixedly connected with the dispensing disc 6 through the connecting rod, the axis of the supporting shaft coincides with the axis of the body 1, and the third driving part 11a drives the supporting shaft to rotate so as to drive the dispensing disc 6 to rotate.

Alternatively, for the third driving portion 11a, a driving motor is disposed on the supporting seat 11b, and the driving motor drives the supporting shaft to rotate so that the connecting rod drives the dispensing disc 6 to rotate around the axis of the body 1. Or may be a structure in which the motor is matched with the synchronous pulley, the output shaft of the motor is connected with the first pulley and the second pulley is located on the supporting seat 11b, the first pulley and the second pulley are connected through the first synchronous belt, and the supporting shaft coaxially connected with the second pulley at least partially penetrates through the shell 11, so that the supporting shaft located in the accommodating cavity 111 is fixedly connected with the dispensing disc 6 through the connecting rod, and the axis of the supporting shaft coincides with the axis of the body 1. The rotation of the dispensing tray 6 can be controlled more accurately through the cooperation of the first synchronous belt, and the precision of the reaction vessel 5 moving to different stations is improved.

In an embodiment, corresponding to the position of the cup feeding station 1a of the body 1, the shell 11, the top cover 12 and the incubation plate 13 are correspondingly provided with the cup feeding opening 112, so that an operator can conveniently and quickly place the reaction container 5 in the accommodating position 61 corresponding to the cup feeding opening 112. The reaction vessel 5 includes a vessel body 51 and a vessel shoulder 52 protruding radially outward, the vessel shoulder 52 being located above the vessel 61 when the reaction vessel 5 is placed on Rong Zhiwei, and the bottom surface of the vessel shoulder 52 being in contact with the surface of the dispensing tray 6 to support the reaction vessel 5 on the dispensing tray 6.

To facilitate placement of the reaction vessel 5 in the receiving place 61 or removal of the reaction vessel 5 from the receiving place 61, the receiving place 61 may be a recess provided at the rim of the dispensing tray 6 and having an opening 611 from which the reaction vessel 5 can be placed or removed in a radial direction. The containing position 61 is directly arranged in the form of a groove structure formed by the opening 611 at the edge of the dispensing disc 6, so that the reaction vessel 5 can be directly extruded into the groove from the cup inlet 112 of the cup inlet station 1a in the rotating process of the dispensing disc 6, and the reaction vessel 5 is connected to the dispensing disc 6 through the matching of the vessel shoulder 52 and the dispensing disc 6, and the structure is simple, so that the cup inlet efficiency is convenient to improve.

Optionally, when the reaction vessel 5 enters the accommodating position 61, in order to facilitate the completion of the cup feeding operation at the cup feeding station 1a, the sample feeding efficiency is improved, and on the basis of setting the accommodating position 61 to the above structure, the size of the accommodating position 61 is set to be larger than the size of the reaction vessel 5, that is, a fit gap is formed between the accommodating position and the accommodating position, and the accommodating position is supported on the dispensing tray 6 through the vessel shoulder 52 of the reaction vessel 5, so as to facilitate the cup feeding.

In an embodiment, the detection station 1b is provided with a positioning assembly 4, and the positioning assembly 4 includes a first driving portion 41 and a connected clamping portion 42, where the first driving portion 41 can drive the clamping portion 42 to move, and the clamping portion 42 can be clamped with the reaction container 5 to position the reaction container 5. Through the locating component 4 that sets up, can carry out auxiliary positioning to the reaction vessel 5 that moves to detection station 1c, can improve the precision that divides the injection disk 6 to drive the reaction vessel 5 motion in-process stop position, avoid reaction vessel 5 to stop the condition that appears the position skew easily when corresponding the station.

Alternatively, when there is a fit gap between the reaction vessel 5 and the accommodation site 61, the reaction vessel 5 enters the accommodation site 61 corresponding to the position of the cup inlet 112 through the cup inlet station 1 a. When the dispensing disc 6 drives the reaction container 5 to enter the detection station 1b, the dispensing disc 6 is controlled to stop rotating, so that the container opening of the reaction container 5 can be opposite to the detection position of the detection station 1 c. However, since the reaction vessel 5 has a fit clearance with respect to the accommodating portion 61, when the rotation of the dispensing disc 6 is controlled, the position of the reaction vessel 5 with respect to the accommodating portion 61 is different from the position of the reaction vessel during the movement, which may cause a deviation of the reaction vessel 5 finally positioned at the detection position, and may cause a problem that the detection cannot be completed. Therefore, the positioning assembly 4 is provided to assist positioning, the dispensing tray 6 drives the reaction vessel 5 to rotate to the detection station 1c, and the clamping portion 42 moves to be clamped with the reaction vessel 5 to limit the relative movement between the reaction vessel 5 and the accommodating position 61. Therefore, the clamping part 42 plays a limiting role on the reaction container 5, so that the reaction container 5 can be more accurately positioned to a detection position, and the deviation is avoided.

Specifically, along with the rotation of the dispensing tray 6, the reaction vessel 5 subjected to sample addition and mixing can be driven to continue to rotate from the sample addition and mixing station 1b to the direction in which the detection station 1c is provided. When the reaction vessel 5 is detected to reach the detection station 1c, the first driving part 41 is controlled to drive the clamping part 42 to act, and the clamping part 42 moves in the radial direction of the body 1 to the direction approaching the detection station 1c until the clamping part 42 is clamped with the reaction vessel 5 corresponding to the detection station 1 c. Through the joint of joint portion 42 and reaction vessel 5 for reaction vessel 5 can accurate location to the testing position in the testing station 1c, improves positioning accuracy. In addition, when the dispensing tray 6 is stopped at the detection station 1c, if the dispensing tray 6 cannot be stopped in time due to inertia, the relative positions of the reaction containers 5 at the storage position 61 are different between the movement state and the stop state of the dispensing tray 6, so that the accuracy of the reaction containers 5 at the detection position after the stopping of the dispensing tray 6 is affected, and the arrangement of the clamping portion 42 can make up for the defect, so that the positioning accuracy of the reaction containers 5 at the detection station 1c is ensured.

Optionally, the incubation plate 13 is at least located on the bottom wall and the side wall of the housing 11, and the incubation plate 13 located on the side wall is in clearance fit with the edge of the dispensing plate 6, so that the reaction vessels 5 in the holding position 61 can be limited to be separated during rotation of the dispensing plate 6. The positioning assembly 4 is disposed on the bottom wall of the incubation tray 13 in the accommodating cavity 111, and the first driving portion 41 is fixedly connected to the incubation tray 13, and the first driving portion 41 is controlled to drive the clamping portion 42 to move along the radial direction so as to clamp or release the clamping with the corresponding reaction container 5.

For the detection station 1c, a detection component is arranged at the position of the corresponding body 1, and at least one through hole is arranged at the position of the top cover 12 corresponding to the detection station 1c, when the reaction container 5 runs to the detection station 1c, the through hole can be opposite to the reaction container 5, and the detection component is used for detecting the mixed liquid in the reaction container 5 through the through hole. In order to make the accommodating cavity 111 have the effects of heat preservation and shading, the detection component comprises a housing 10 arranged on the top cover, the housing 10 shields the through hole, and the detection component such as a detector for detection is arranged inside the housing 10. The detector for detecting the mixed liquid may be, for example, a light detection unit that is fixed to the top cover 12 or the housing 11, and detects the mixed liquid in the reaction vessel 5 by passing a laser through the through hole.

It will be appreciated that the dispensing disc 6 rotating around the axis of the body 1 drives the reaction vessel 5 to rotate, and when it is detected that the reaction vessel 5 rotates to the detection station 1c, it is necessary to stop rotating by controlling the dispensing disc 6 so as to position the reaction vessel 5 at the detection station 1c. Due to the deviation of precision problems such as control rotation and matching caused by long-term wear of equipment, the reaction vessel 5 is easy to generate position deviation positioned at the detection station 1c, so that the relative positions of the through holes and the reaction vessel 5 gradually deviate, and the detection of the mixed liquid in the reaction vessel 5 is influenced. Therefore, the positioning assembly 4 is arranged at the position of the accommodating cavity 111 closer to the detection station 1c, so that when the reaction vessel 5 subjected to sample adding and mixing is detected to rotate to the detection station 1c, a stop command is sent to the third driving part 11a for stopping rotation by controlling the dispensing disc 6, and meanwhile, the command is sent to the first driving part 41 of the positioning assembly 4, the first driving part 41 and the third driving part 11a act simultaneously, so that the dispensing disc 6 stops moving, and meanwhile, the clamping part 42 is clamped with the reaction vessel 5, double positioning of the reaction vessel 5 is realized, and the accuracy and stability of positioning of the reaction vessel 5 at the detection station 1c are improved.

As a specific embodiment of the present application, referring to fig. 4 and 5, for the provided clamping portion 42, the clamping portion 42 may include a clamping block 421 provided with a clamping groove 422, where the clamping block 421 is connected to the output end of the first driving portion 41, and can drive the clamping block 421 to move in the radial direction until the clamping groove 422 abuts against the reaction container 5. The first driving portion 41 can push the clamping block 421 to approach or depart from the side wall of the incubation plate 13 along the radial direction of the body 1, and a clamping groove 422 is formed on one side of the clamping block 421 facing the side wall of the incubation plate 13, and the clamping groove 422 can be, for example, a V-shape or an arc shape that is concave inwards along the radial direction of the body 1. The structure is simple to cooperate and easy to realize.

Alternatively, the clamping groove 422 is arranged in a symmetrical V-shaped structure, and the outer wall of the side, facing the clamping groove 422, of the reaction container 5 is an arc-shaped surface. The clamping grooves 422 in the V shape can adapt to the reaction containers 5 in different shapes to realize clamping fit, and the positioning assembly 4 does not need to be replaced. Moreover, the clamping groove 422 in the V shape can better clamp the reaction container 5 through the two side surfaces of the V shape, so that the positioning precision is improved, and the shaking is avoided.

Alternatively, the locking portion 42 may include a mating fastener and a mating hook, one of which is disposed at the output end of the first driving portion 41, and the other of which is disposed at the reaction container 5. The output end of the first driving part 41 may be connected with a supporting block, the buckle is arranged on the supporting block, and the hook is arranged on the outer side wall of the reaction container 5. The first driving part 42 acts to drive the buckle to move along the radial direction of the body 1 and close to the incubation plate 13 until the buckle and the clamping hook are clamped, so that the reaction container 5 is positioned at the detection station 1c through the clamping of the buckle and the clamping hook. Optionally, for the matched buckle and the hook that are set, for example, a matching structure that is realized through automatic control may be set, the buckle is an annular or hook-shaped structure that is set in the reaction container 5, and when the buckle is detected to be abutted with the preset position of the hook, the hook may be controlled to rotate to realize buckling with the buckle. When the detection is completed, the hook is controlled to reversely rotate to be disengaged from the buckle.

It should be emphasized here that the engaging portion 42 may be provided in other engaging structures that can be engaged and disengaged, and this is not a mere example.

And to the first drive portion 41 that sets up, in order to improve the positioning accuracy and the positioning stability of joint portion 42, first drive portion 41 can include motor 411, motor 411 fixed connection is in the accommodation chamber 111 of body 1, the guide portion includes complex screw rod 412 and slider 413, the extending direction of screw rod 412 is parallel with body 1 radial direction, slider 413 swing joint is in screw rod 412, motor 411 can drive the screw rod 412 rotation of connection, so as to drive slider 413 along the extending direction motion of screw rod 412, thereby make joint portion 42 can realize the joint to reaction vessel 5 or release the joint. Through setting up the structure cooperation form of screw nut pair for the rotation of motor 411 drive screw 412 can drive the slider 413 with screw 412 screw-thread fit and slide along body 1 radial direction, and this kind of structure cooperation can be more accurate control slider 413's direction of motion and motion distance, avoids joint portion 42 to reduce joint's stability and precision because of excessive motion or lack motion.

In an alternative embodiment, the positioning assembly 4 may further include a travel control portion 414, configured to control the reciprocation of the slider 413, where the travel control portion 414 may be a travel switch disposed at the bottom of the incubation tray 13, and when the clamping portion 42 is clamped with the reaction container, the travel switch sends a signal to control the clamping portion 42 to stop moving, so as to avoid the excessive movement of the slider 413, resulting in the clamping portion 412 extruding the reaction container 5, so as to deform or damage the reaction container; meanwhile, the control clamping part 42 is kept in a static state in a set time, so that the stability of the reaction container 5 is ensured when the detection device detects a sample to be detected in the set time. When the set time is reached, the motor 411 is controlled to rotate reversely, and the slider is retracted, so that the locking portion 413 is released from locking with the reaction vessel 5.

It should be understood that the stroke control portion 414 may be other structures that can achieve the control purpose, such as an optocoupler, which is not illustrated herein.

Optionally, no matter what kind of structural cooperation form is set to the positioning assembly 4, when the clamping portion 42 cooperates with at least one reaction container 5 to position the reaction container 5 with the mixed liquid at the detection station 1c, in order to be convenient for controlling the rotation of the dispensing tray 6 so that the reaction container 5 can be precisely positioned at different stations, the plurality of holding positions 61 are uniformly arranged along the circumferential direction of the dispensing tray 6, and a preset angle is formed between adjacent holding positions 61, and each time the dispensing tray 6 rotates by a preset angle, the clamping portion 42 is controlled to be clamped with the reaction container 5. The preset angle between the adjacent accommodating portions 61 may be adaptively adjusted according to specific conditions such as controlling the rotation speed of the dispensing tray 6, the angle between different stations, or controlling the reaction speed of the actions of each driving portion, which is not particularly limited herein.

In one embodiment, referring to fig. 6 and 7, for the discard cup station 1d of the body 1, the reaction vessel 5 containing the mixture after detection is guided out of the dispensing tray 6 and discarded into the collection device, and this is achieved by the discard cup assembly 2 provided at the discard cup station 1 d. The cup discarding assembly 2 comprises a second driving part 22 and a pushing part 23 which are matched, the second driving part 22 is used for driving the pushing part 23 to move, and when the reaction container 5 moves to the cup discarding station 1d, the pushing part 23 is used for separating and pushing the reaction container 5 from the dispensing tray 6 to a preset position. The disposal cup assembly 2 is beneficial to the more stable and more accurate disposal of the reaction vessel 5 to a fixed position at the disposal cup station 1 d.

Wherein, the pushing part 23 is connected to the matching component 221, the matching component 221 is used for driving the pushing part 23 to move, the driving component 222 can provide power for the movement of the matching component 221, when the reaction container moves to the cup discarding station 1d, the driving component 222 can drive the matching component 221 to move, the matching component 221 drives the pushing part 23 to move, and the pushing part 23 is used for separating and pushing the reaction container 5 from the dispensing tray 6 to a preset position. The second driving part 22 is formed by a separate arrangement of the driving assembly 222 and the mating assembly 221. If the pushing unit 23 is directly driven by the driving mechanism, the driving mechanism in high-speed operation cannot be stopped immediately after receiving a start or stop command, and the pushing unit 23 may be stopped if it moves excessively or does not reach the set position. Therefore, by providing the matched driving assembly 222 and the matched assembly 221, the matched assembly 221 is utilized as buffering, so that the positioning accuracy of the pushing part 23 can be controlled more accurately and stably, and the problem of low positioning accuracy of the driving mechanism is solved. In addition, the arrangement of the driving assembly 222 and the matching assembly 221 improves the positioning accuracy of the pushing part 23, and simultaneously enables the reaction vessel 5 to be smoothly separated from the dispensing tray 6 and pushed in the first channel 213, so that the pushing part 23 is prevented from extruding and jamming the reaction vessel 5 in the first channel 213 under the condition of low positioning accuracy.

The cup discarding assembly 2 comprises a mounting bracket 21, the mounting bracket 21 is connected to the body 1 at a position corresponding to the cup discarding station 1d, the mounting bracket 21 is provided with a first channel 213 communicated with the accommodating cavity 111, a second driving part 22 is connected to the mounting bracket 21, a pushing part 23 is connected with the second driving part 22, and the second driving part 22 is used for driving the pushing part 23 to move.

It will be appreciated that the body 1 is provided with a cup outlet 113 penetrating the housing 11 and the incubation plate 13 corresponding to the cup discarding station 1d, the cup outlet 113 is communicated with the first channel 213, the first channel 213 extends along the radial direction of the body 1 and passes through the cup outlet 113 to the dispensing plate 6, the pushing portion 23 can separate the reaction vessel 5 from the dispensing plate 6 and enter the first channel 213, and the pushing portion 23 can push the reaction vessel 5 to move along the extending direction of the first channel 213. The reaction vessel 5 rotated to the cup discarding station 1d is opposite to the through opening 113, so that the extending direction of the reaction vessel 5 and the first channel 213 is in a straight line, and the pushing part 23 can smoothly release the reaction vessel 5 from the opening 611 of the dispensing tray 6 along the radial direction of the body 1 as long as the pushing part 23 is in contact with at least part of the reaction vessel 5 and applies a force in a direction away from the axis of the body 1, and directly enters the first channel 213 along with the pushing of the pushing part 23. The cooperation structure is simple, the reaction vessel 5 can be pushed conveniently, and the work of discarding the reaction vessel 5 from the first channel 213 to the collecting device can be completed well.

In an alternative embodiment, the mating assembly 221 includes a mating guide rail 221a and a sliding block 221b, the guide rail 221a extends along the radial direction of the body 1, the pushing portion 23 is connected to the sliding block 221b, and the movement of the sliding block 221b along the guide rail 221a can drive the pushing portion 23 to move along the extending direction of the first channel 213. By arranging the matched guide rail 221a and the sliding block 221b, the power provided by the driving assembly 222 is converted into the sliding of the sliding block 221b relative to the guide rail 221a, so that the movement precision of the sliding block 221b relative to the guide rail 221a can be better controlled, the displacement precision of the pushing part 23 is further improved, the pushing part 23 can accurately separate the reaction container 5 from the dispensing disc 6 and push the reaction container 5 to the first channel 213, the discarding work of the reaction container 5 is smoothly realized, and the reaction container 5 is prevented from being accumulated in the first channel 213.

The driving assembly 222 includes a driving motor 222a and a synchronous pulley set, the synchronous pulley set includes a first synchronous pulley 222b, a second synchronous pulley 222c and a second synchronous belt connecting the two, the first synchronous pulley 222b is connected to an output shaft of the driving motor 222a, the second synchronous pulley 222c is connected to the mounting bracket 211, the sliding block 221b is connected to the second synchronous belt, a plane formed by a connecting line between an axis of the first synchronous pulley 222b and an axis of the second synchronous pulley 222c is parallel to an extending direction of the guide rail 221a, and the driving motor 222a can drive the first synchronous pulley 222b to rotate, so that the matched second synchronous belt drives the sliding block 221b to slide along the guide rail 221 a. The rotation of the driving motor 222a is converted into the movement of the synchronous belt through the synchronous belt group connected with the output shaft of the driving motor 222a, the movement speed is far lower than the rotation speed of the driving motor 222a, and the movement precision of the sliding block 221b is higher through controlling the movement of the synchronous belt to avoid the conditions of under-movement or over-movement of the pushing part 23 and the like by controlling the movement of the synchronous belt to control the movement of the sliding block 221 b.

Optionally, the second driving portion 22 further includes a limiting assembly, where the limiting assembly is used to limit the movement of the sliding block 221b, and the limiting assembly includes a stroke block 221c and a stroke control, where the stroke block 221c is connected to the sliding block 221b, and the stroke control is located on two sides of the stroke block 221c along the extending direction of the guide rail 221 a. When the sliding block 221c moves along the guide rail 221a, through the cooperation of the set travel control and the travel block 221c, the sliding block 221b can avoid that the sliding block 221b drives the pushing part 23 to move excessively when the driving assembly 222 fails, the movement of the pushing part 23 is limited to reciprocate in a limited range, and the stability and the precision of the movement of the pushing part 23 are improved. The stroke control may be an abutment block, in which the movement of the sliding block 221c is limited by abutment of the stroke block 221c with the abutment block, or may be a photosensitive automatic control structure such as an optocoupler to limit the sliding block 221c, and the relative distance of the stroke block 221c is measured by light formed by the optocoupler 3. Alternatively, other stroke control capable of realizing the limit may be provided, and the limitation is not particularly limited.

Specifically, the mounting bracket 21 includes a mounting plate 211 disposed thereon, the mounting plate 212 is connected to one side of the body 1, the mounting plate 211 extends along a radial direction of the body 1 and partially extends out of the mounting plate 212 to above the body 1, the second driving portion 22 is located on the mounting plate 211, the first channel 213 is located on the mounting plate 212, the mounting plate 211 is provided with a movement channel 211a, the movement channel 211a is communicated with the first channel 213, and the pushing portion 23 at least partially penetrates through the movement channel 211a. Through setting up mounting panel 211 and mount pad 212 of cooperation for second drive portion 22 can drive the drive portion 23 in the reciprocating motion in-process, with reaction vessel 5 from the injection dish 6 release, drive the reaction vessel 5 of release get into first passageway 213 and promote to fixed collecting position along first passageway 213 and accomplish and abandon the cup work, avoid reaction vessel 5 to pile up in first passageway 213 or not separate from injection dish 6.

It will be appreciated that the purpose of the mounting plate 211 and the mounting base 212 based on the cooperation is to be able to better achieve the cooperation between the second drive part 22, the pushing part 23, the dispensing disc 6 and the reaction vessel 5 thereon and the first channel 213, to be able to push the reaction vessel 5 stably to the collecting position without accumulating in the first channel 213. So for the disposed disposal cup assembly 2, the specific structural cooperation between the parts may be, for example: the mount pad 212 sets up in the lateral part that body 1 corresponds to abandoning cup station 1d, and the mount pad 212 is equipped with along the radial first passageway 213 that extends of body 1 towards one side of body 1, and first passageway 213 extends to stretching into holding chamber 111 and its interior filling tray 6 clearance fit through play rim of a cup 113, and when reaction vessel 5 rotates to abandoning cup station 1d along with filling tray 6, the holding bit 61 opening 611 that is equipped with reaction vessel 5 is relative with first passageway 213. The portion of the mounting plate 211 above the mounting seat 212 extends at least above the dispensing tray 6 corresponding to the reaction vessel 5 along the radial direction of the body 1, so that when the sliding block 221c drives the pushing portion 23 to move, the pushing portion 23 at least can move above the accommodating position 61, and the reaction vessel 5 is pushed to be separated from and enter the first channel 213 after being abutted to one side, away from the opening 611, of the reaction vessel 5 by the pushing portion 23. The pushing portion 23 is disposed above the reaction container 5, so as to improve stability and strength of applying force to the reaction container 5, and the length of extension of the corresponding guide rail 221a, the specific positions of the limiting assemblies disposed on two sides of the sliding block 221c, the specific arrangement positions of the two synchronous pulleys in the driving assembly 222, the length and position of extension of the movement channel 211a along the radial direction of the body 1, and the like can be adaptively adjusted according to the setting of the pushing portion 23, which is not limited herein.

When the reaction vessel 5 rotates to the cup discarding station 1d, since the reaction vessel 5 has the above-mentioned cup structure with the vessel shoulder 52, the pushing portion 23 has a good cup pushing effect on the reaction vessel 5, so that the reaction vessel 5 can be quickly and stably pulled out from the opening 611 to the first channel 213 along the radial direction of the body 1. The pushing portion 23 includes a dial 232 and a base 231 connecting the dial 232 to the slider 221b, the dial 232 passing through the movement channel 211a, the dial 232 having an abutment surface 232a for abutment with a side wall of the container shoulder 52 to push the reaction container 5 into movement. Before the reaction container 5 reaches the cup discarding station 1d, the shifting block 232 can move to a position above the cup discarding station 1d corresponding to the accommodating position 61, and the shifting block 232 is at least partially positioned on one side of the accommodating position 61 away from the second driving part 22, so that when the reaction container 5 reaches the cup discarding station 1d, one side of the reaction container 5 away from the opening 611 can be abutted with the abutting surface 232a, and the movement of the shifting block 232 in the direction close to the opening 611 can drive the reaction container 5 to separate from the dispensing tray 6.

Alternatively, the surface of the side of the dial 232 facing the first channel 213 is provided with a recess 232b when the dial 232 cooperates with the reaction vessel 5 having the vessel shoulder 52 to push it out of engagement from the dispensing disc 6, such that the dial 232 forms two opposing protruding structures in the radial direction of the body 1, the abutment surface 232a being provided on the opposite side wall of at least one of the protrusions. In operation of the disposable cup assembly 2, at least part of the container shoulder 52 of the reaction container 5 is located in the recess 232b, the abutment surface 232a corresponding to the reaction container 5 in the radial direction of the body 1. When the dial 232 is moved to separate the reaction container 5 from the dispensing tray 6, at least the abutment surface 232a on the side away from the opening 611 abuts against the reaction container 5 and pushes the reaction container 5 to move in the radial direction to enter the first passage 213.

For the setting block 232, the surface thereof facing the side of the first passage 213 may be flush or clearance-fitted with the lower surface of the container shoulder 52 of the reaction container 5 at the time of dispensing disc 6. When the two are in clearance fit, the surface of the shifting block 232 facing the side of the first channel 213 is located above the lower surface of the container shoulder 52, as seen in the height direction of the incubation unit, and the clearance between the two surfaces is not greater than half the height of the container shoulder 52.

In an alternative embodiment, the pushing portion 23 may also be provided as two clamping blocks that can be close to or far away from each other along the radial direction of the body 1, the movement of the two clamping blocks that can be close to or far away from each other may be controlled by a driving member that is provided, before the reaction vessel 5 enters the cup discarding station 1d, the pushing portion 23 is located above the dispensing tray 6, and the two clamping blocks are respectively located at two sides of the reaction vessel 5 to be entered, until the reaction vessel 5 enters the cup discarding station 1d, the two clamping blocks can be controlled to clamp the reaction vessel 5, and then the reaction vessel 5 can be conveniently and rapidly separated from the dispensing tray 6 into the first channel 213 by controlling the second driving portion 222. The pushing portion 23 may be provided with other engaging structures capable of ensuring pushing of the reaction vessel 5 to the collecting position, and stacking in the passage is avoided, which is not shown here.

In an embodiment, along the extending direction of the first channel 213, the first channel 213 includes a first sub-channel 213a and a second sub-channel 213c that are connected, the first sub-channel 213a is located above the second sub-channel 213c, and the width of the first sub-channel 213a is greater than the width of the second sub-channel 213c, so that a step surface 213b is formed therebetween, and along the height direction of the incubation unit, the step surface 213b is flush or in clearance fit with the surface of the dial block 232 close to the first channel 213. The first sub-passage 213a and the second sub-passage 213c are provided, and the shape of the cross section thereof is adapted to the shape of the reaction vessel 5 in the extending direction of the first passage 213, and the width of the second sub-passage 213c is equal to or larger than the diameter of the vessel body 51 while being smaller than the width of the vessel shoulder, and is at most not larger than 5mm of the diameter of the vessel body 51. The width of the first sub-channel 213a is greater than the diameter of the container shoulder 52. When the pushing part 23 pushes the reaction container 5 to enter the first channel 213 through the matched design of the first sub-channel 213a and the second sub-channel 213c, the reaction container 5 can be limited in the channel with limited space, and the lower surface of the container shoulder 52 in the reaction container 5 is contacted with the step surface 213b, so that when the pushing part 23 pushes the reaction container 5 to enter the first channel 213, the reaction container 5 can directly enter the first channel 213 along the radial direction, and the situation that skew occurs due to uneven stress when pushing the reaction container 5 is avoided through a shape-adapting mode, so that the reaction container 5 is accumulated in the first channel 213 and cannot be pushed to a fixed collecting position by the pushing part 23.

Optionally, the mounting base 212 further includes a second channel 214 communicating with the first channel 213, the second channel 214 intersecting the first channel 213, and the pushing portion 23 is capable of pushing the reaction vessel 5 from the first channel 213 into the second channel 214. For the second passage 214 provided for communication with the collecting means for collecting the used reaction vessel 5, the diameter of the second passage 214 is at least larger than the height of the reaction vessel 5 so that the reaction vessel 5 can smoothly enter the collecting means from the second passage 214. The extending direction of the second passage 214 may be perpendicular to the extending direction of the first passage 213 or inclined downward at a predetermined angle, so long as the reaction vessel 5 is not stacked or caught in the second passage 214, and is not particularly limited.

It will be appreciated that the predetermined positions referred to above may be different according to different mating structures such as the first channel 213 and the second channel 214 provided in the mounting base 212. For example, when the first channel 213 and the second channel 214 intersecting with each other are provided, the predetermined position is the intersection position where the two channels intersect. As long as the reaction vessel 5 can be pushed to a predetermined position while the reaction vessel 5 can be directly or indirectly introduced into the collecting device, there is no particular limitation.

It will be appreciated that in the process of rotating the dispensing tray 6, the position corresponding to each station is provided with an in-place detection device such as an optocoupler, and the control unit can complete sample analysis according to the set in-place detection device and the combination of components in each station in time sequence. Taking a reaction container 5 as an example, when the dispensing disc 6 rotates to an empty containing position 61 opposite to the cup inlet 112, the in-place detection device sends a signal to the control unit, and the control unit controls the third driving part 11a to stop driving the dispensing disc 6 to rotate until the cup inlet is completed. The sample is sequentially passed through the sample-adding and mixing station 1b and the detecting station 1c according to the same principle, and will not be described in detail here. When the in-place detecting device detects that the reaction vessel 5 reaches the cup discarding station 1d, the third driving part 11a is controlled to stop, in this state, the pushing part 23 in the cup discarding assembly 2 has moved to above the accommodating position 61 in advance, and when waiting for the reaction vessel 5 to enter the cup discarding station 1d, the reaction vessel 5 is provided with two convex structures of the shifting block 232 on both sides of the extending direction of the first channel 213, and the abutting surface 232a of the shifting block 232 abuts against or is in clearance fit with the side wall of the corresponding vessel shoulder 52 of the reaction vessel 5. And the second driving part 222 is driven to drive the matching component 221 to move, so that the reaction vessel 5 is driven to deviate from the opening 611 of the accommodating position 61 and enter the first channel 213 to a preset position, and the cup throwing of the reaction vessel 5 is realized.

In an embodiment, the position detecting assembly 3 may further comprise a position detecting assembly 3, wherein the position detecting assembly 3 comprises at least one detecting part 31 at least positioned on the cup discarding assembly 2, and the detecting part 31 is at least used for detecting the position of the reaction container 5 in the cup discarding assembly 2. Through the detection portion 31 that sets up, can detect the condition that reaction vessel 5 moved in abandoning cup subassembly 2 at least, can judge whether to have the reaction vessel 5 of promotion to appear not promoting in place and detain in its interior condition when abandoning cup subassembly 2 removal according to the result of detection to ensure can not cause reaction vessel 5 local jam and influence the normal use of incubation unit when throwing the cup, can be convenient for the staff simultaneously can in time solve the jam problem in order to get rid of the trouble.

Optionally, the detecting portion 31 is located at least at a position corresponding to the first channel 213, and is configured to detect a position of the reaction vessel 5 in the first channel 213. When the detecting portion 31 is disposed at the position corresponding to the first channel 213, the position of the reaction vessel 5 pushed by the pushing portion 23 in the first channel 213 can be detected by the detecting portion 31 to determine whether the reaction vessel 5 is pushed in place for a certain time and stays in the first channel 213, so that local congestion of the reaction vessel 5 is not caused during cup throwing, and normal use of the incubation unit is not affected, and meanwhile, workers can solve congestion problems in time to remove faults.

As for the provided detecting portion 31, the detecting portion 31 is located at least at a corresponding position on the side of the first passage 213 away from the dispensing tray 6 in the extending direction of the first passage 213, and the detecting portion 31 is used to detect the reaction vessel 5 passing through the position. The detecting part 31 is arranged at a position relatively far away from the body 1 in the first channel 213, so that when the reaction vessel 5 moves in the first channel 213 during the cup pushing process of the cup discarding station 1d, when the detecting part 31 arranged at one end far away from the body 1 can detect that the reaction vessel 5 passes through the position, the first half section is not blocked by the reaction vessel 5, so that the reaction vessel 5 can finally enter the collecting device. When the pushing part 23 does not detect the reaction vessel 5 at the preset position during the pushing process, it is proved that the reaction vessel 5 remains in the first channel 213, and the pushing part 23 does not push it into place, which easily results in the reaction vessel 5 being stacked in the first channel 213. At this time, the detecting part 31 can send out an alarm signal so that the staff can remove the fault in time, and the normal sample analysis work is prevented from being influenced.

Alternatively, a plurality of detecting portions 31 may be included to be sequentially arranged along the extending direction of the first passage 213, and the plurality of detecting portions 31 may be used to detect a specific position of the reaction vessel 5 moving the first passage 213 before the reaction vessel 5 moves to a preset position along the first passage 213. In order to realize the real-time monitoring to the reaction vessel 5, can learn the position of the reaction vessel 5 in different time in this time period of the movement of the first channel 213 and whether the reaction vessel 5 leaves the position at the next moment to enter the next position, improve the detection effect to the position state of the reaction vessel 5, can discover the problem in time, avoid crowding in the first channel 213.

When the disposal cup assembly 2 is provided with the first channel 213 and the second channel 214 which are communicated, the second channel 214 is used for guiding the reaction vessel 5 into the collecting device, and the detecting portion 31 may also be disposed at a position corresponding to the second channel 214 for detecting the position of the reaction vessel 5 in the second channel 214. Through the setting of the corresponding position of the second channel 214 that sets up the detection portion 31, after this detection portion 31 detects that the reaction vessel 5 exceeds the time of predetermineeing in this position, then send out the warning, probably be that collection device fills reaction vessel 5 and appears overflowing to the condition of second channel 214, remind the staff to handle in time, guarantee the normal operating of incubation unit, avoid filling up collection device and flowing backward to first channel 213 and cause piling up because of the maloperation.

It will be appreciated that, for the detection portion 31 provided, it is also possible to provide a position of the mounting plate 211 in the cup reject assembly 2 corresponding to the pushing portion 23, the detection portion 31 being configured to detect the movement position of the pushing portion 23. The detection part 31 directly detects the position of the pushing part 23 to ensure that the pushing part 23 can move in place in the reciprocating motion process so as to push the reaction container 5 to a fixed position, thereby ensuring that the reaction container 5 is not accumulated in the first channel 213 to cause blockage.

The detecting unit 31 itself may be configured to recognize by light sensation, or may be configured to recognize by contact, that is, may be provided at a position of the housing 11 corresponding to the cup discarding station 1d, and is not particularly limited herein, for detecting whether the reaction container 5 reaches the cup discarding station 1 d.

In an alternative embodiment, referring to fig. 8, the detecting portion 31 includes a probe 311, and the cup discarding assembly 2 has an alignment hole 212a communicating with the first channel 213, and the probe 311 extends at least partially into the alignment hole 212a. Through the alignment hole 212a, the probe 311 can detect the position of the reaction vessel 5 in the first channel 213, and when the reaction vessel 5 moves to the position corresponding to the probe 311 along the first channel 213, the probe 311 can sense the reaction vessel 5, thereby measuring the position of the reaction vessel 5. For example, when the detecting portion 31 is provided in a mechanically fitted structure, the probe 311 has a contact surface, and when the reaction vessel 5 moves along the first passage 213, the contact surface of the probe 311 can be brought into contact with the reaction vessel 5, and the reaction vessel 5 can continue to move relative to the contact surface to release the contact. For another example, when the detecting portion 31 is provided in a photoelectric sensing type structure, the probe 311 can form a beam of light to be injected into the first passage 213 through the alignment hole, and the reaction vessel 5 can break the beam of light when the reaction vessel 5 moves to this position along the first passage 213. By forming a complete beam of light in the first channel 213, the reaction vessel 5 will form a barrier when the reaction vessel 5 passes this position, so that the formed beam of light is broken, whereby the position of the reaction vessel 5 in the first channel 213 can be determined.

Optionally, when the detecting portion 31 is configured as a photoelectric sensing structure, in order to achieve a better position detection effect, the detecting portion 31 is an optical coupler, the alignment holes 212a are symmetrically disposed on two sidewalls of the first channel 213, along the extending direction perpendicular to the first channel 213, two output ends of the optical coupler are located on two symmetrical sides of the first channel 213, that is, located at positions of the corresponding alignment holes 212a, respectively, and the two output ends form a light beam in the first channel 213, and when the reaction vessel 5 moves to the position, the reaction vessel 5 breaks the light beam to represent the position where the reaction vessel 5 moves at this time, so that position location can be performed.

In an embodiment, when the reaction vessel 5 is the structure having the vessel body 51 and the vessel shoulder 52 as described above and the first channel 213 is provided in a stepped structure including the first sub-channel 213a and the second sub-channel 213c adapted to the reaction vessel 5 of the structure, the alignment hole 212a for providing the probe 311 may be provided in a sidewall of at least one of the first sub-channel 213a and the second sub-channel 213c for the provided probe portion 31 such that the alignment hole 212a penetrates at least one of the first sub-channel 213a and the second sub-channel 213 c. When the alignment hole 212a is provided at a position corresponding to the first sub-passage 213a, the probe 311 at least partially protruding into the alignment hole 212a is adapted to cooperate with the container shoulder 52 of the reaction container 5 to determine whether the reaction container 5 is stacked in the first passage 213 by detecting whether the container shoulder 52 passes this position within a set interval time, stays at this position for more than a preset time, or the like. Similarly, when the alignment hole 212a is disposed at a position corresponding to the second sub-channel 213c, the probe 311 at least partially extending into the alignment hole 213a is used to cooperate with the container body 51 of the reaction container 5, which is not described in detail herein.

In an alternative embodiment, the detecting portion 31 may further include an identifying unit, in the process of moving the reaction container 5 along the first channel 213, the detecting portion 31 may determine, according to a preset time, the number of times that the reaction container 5 passing through a position corresponding to the setting detecting portion 31 passes through, to determine whether a situation that the reaction container 5 is not pushed in place occurs when being pushed to move for a certain time, so that the number of times that the reaction container 5 actually passes through is smaller than the preset number of times, thereby ensuring that local congestion of the reaction container 5 is not caused when the reaction container is thrown, and normal use of the incubation unit is not affected, so that a worker can timely remove a fault. For example, the identification unit may include at least one of a counter and a timer, which are electrically connected to the probe 311, respectively, for limiting the number of times the reaction container 5 is detected within a preset time to a preset number of times, and for limiting the time of the detected reaction container 5 to a preset time range. By providing a counter, a timer, or the like, the in-place detection of the reaction vessel 5 can be combined with the timing, and whether or not the reaction vessel 5 has accumulated in the first passage 213 can be determined more accurately by the time interval between the passage of the corresponding position of the reaction vessel 5, the number of times of passage of a certain period of time, the time when the reaction vessel 5 is detected at the position, or the like.

Optionally, the position detecting component 3 may be further disposed at the detecting station 1c, the sample adding and mixing station 1b, etc. for detecting whether the reaction container 5 accurately reaches the corresponding station, so as to improve the positioning accuracy, which is not limited herein.

As a specific embodiment of the application, the incubation unit includes a control portion, where the control portion may include a middle position machine and a lower position machine, the middle position machine is configured to receive a signal from the lower position machine and send an instruction to the lower position machine, and the lower position machine is configured to execute an instruction control mechanism of the middle position machine to implement a corresponding action and feed back the executed corresponding action forming signal to the middle position machine. The lower computer may include a control unit, where the control unit is configured to correspond to the electronic components, such as the driving motor, the detector, and the detecting portion, and the electronic components in the foregoing may be connected to the control unit, so that the incubation unit may orderly implement incubation operation, which is not described in detail herein.

To sum up the incubation unit that sets up, the locating component 4 that sets up in the incubation unit can be through its assistance-localization real-time to the dispensing dish 6 for reaction vessel 5 can stop at corresponding station position accuracy higher when rotating along with the dispensing dish 6. The provided cup discarding assembly 2 is advantageous in that the reaction vessel 5 can be discarded to the collecting device more stably and with higher accuracy at the cup discarding station 1 d. The position detection assembly 3 is provided to monitor the position of the reaction vessel 5 in the cup reject assembly 2 to avoid clogging in the cup reject assembly 2. Through the design in integrating a plurality of stations in incubating for incubating the unit has higher integrated level, can conveniently quick change reaction vessel 5 to different stations, and can maintain reaction vessel 5 at suitable temperature all the time in the conversion process, with stability and the degree of accuracy of guaranteeing to incubate the result.

The application also provides a sample analyzer, which includes an incubation unit, wherein the incubation unit is the incubation unit described in the foregoing, and details are not described herein.

Based on the above incubation unit, the present application provides a sample analysis method, taking a reaction vessel 5 as an example for performing a sample analysis process in the incubation unit, the sample analysis method includes:

s101, loading the reaction container 5 from the cup feeding station 1a into the dispensing disc 6 by a loading mechanism.

S102, the reaction vessel 5 enters a sample adding and mixing station 1b, and samples are added into the reaction vessel 5 for mixing.

S103, after the reaction containers 5 subjected to sample adding and mixing are mixed, the reaction containers 5 are driven by the dispensing disc 6 to move to the detection station 1c according to time sequence, when the detection station 1c detects that the reaction containers 5 move to the detection station 1c, detection signals are sent to the positioning component 4, the positioning component 4 receives the detection signals to control the first driving part 41 to move so as to enable the clamping part 42 to be clamped with the reaction containers 5, after detection is finished, the detection station 1c sends a finishing command to the positioning component 4, and the first driving part 41 receives the finishing command to control the clamping part 42 to be clamped with the reaction containers 5.

And S104, the dispensing disc 6 drives the reaction container 5 to continue to move to the cup discarding station 1d according to a time sequence, when the reaction container 5 is detected to move to the cup discarding station 1d, the second driving part 22 is controlled to move so that the pushing part 23 drives the reaction container 5 to be separated from the dispensing disc 6 and move to a preset position, and the detecting part 31 is at least used for detecting the position reached by the pushing part 23 when the reaction container 5 is pushed.

The reaction vessel 5 is loaded from the cup-in station 1a into the dispensing tray 6 by a loading mechanism. The reaction vessel 5 enters the dispensing disc 6 from the cup inlet 112, so that the reaction vessel 5 enters from the opening 611 to be matched with the containing position 61 in the dispensing disc 6, and the rotation of the dispensing disc 6 can drive the reaction vessel 5 to rotate out of the cup inlet station 1a to move to the next sample injection mixing station. It will be appreciated that in this cup feeding station 1a, when the reaction vessel 5 is placed in the accommodating position 61, the control unit may control the dispensing tray 6 to stop at the cup feeding station 1a, and after the accommodating position 61 opposite to the cup feeding opening 112 is placed in the reaction vessel 5 through the opening 611, control the dispensing tray 6 to rotate until the accommodating position 61 of the next placing cavity is opposite to the cup feeding opening 112, so as to realize cup feeding. Or the dispensing tray 6 can be kept in a moving state all the time in the whole cup feeding process, and when the dispensing tray rotates to the position where the cup inlet 112 is opposite to the containing position 61 of the containing cavity, the reaction container 5 is quickly placed in the containing position 61 of the containing cavity by utilizing a time gap, so that the efficiency is improved. The present invention is not particularly limited herein.

The reaction vessel 5 enters the sample-adding and mixing station 1b, and samples are added and mixed into the reaction vessel 5. The dispensing tray 6 is controlled to rotate in time sequence, and when the rotation of the dispensing tray 6 is detected to drive the reaction vessel 5 to enter the sample adding and mixing station 1b, one or more liquids are controlled to be automatically or manually added from the at least one through hole 121 of the top cover 12, so that the added liquids are mixed in the reaction vessel 5 to obtain a mixed liquid. It will be appreciated that the means for loading in the loading and mixing station 1b may be provided as an automatic loading means which is capable of controlling the loading of the sample into the reaction vessel 5 through the through-hole 121 when it is detected that the reaction vessel 5 is moved to this position when the dispensing disc 6 is rotated.

The reaction containers 5 subjected to sample addition and mixing are driven to rotate by the dispensing tray 6 to the detection station 1c in time sequence, and in the detection station 1c, the detection heads or detection rays of the detectors are required to enter from the through holes so as to detect the mixed liquid in the opposite reaction containers 5. In order to ensure the accuracy of the detection, it is necessary to control the dispensing tray 6 to stop so that the mouth of the reaction vessel 5 faces the through hole when the reaction vessel 5 moves to the station. Through the locating component 4 that sets up, when detecting reaction vessel 5 operation to detecting station 1c, control locating component 4 work for joint portion 42 can realize the joint with the branch annotate dish 6, with through joint portion 42 restriction branch annotate dish 6 pivoted auxiliary positioning, improved the counterpoint precision of through-hole and reaction vessel's container mouth.

It will be appreciated that, for the positioning assembly 4 provided, the positioning method thereof specifically comprises: taking one reaction container 5 as an example, the dispensing disc 6 is controlled to drive the reaction container 5 to rotate according to a time sequence, when the reaction container 5 is detected to move to the detection station 1c, a positioning signal is sent to the control unit, when the control unit receives the positioning signal, a control command is respectively sent to the first driving part 41 and the third driving part 11a, after the control command is received by the third driving part 11a, the dispensing disc 6 is controlled to stop rotating, the first driving part 41 controls the clamping part 42 to move, the clamping part 42 is clamped with the reaction container 5 at least positioned at the detection station 1c, after the detection is finished, the control unit receives a detection finishing signal, controls the first driving part 41 to control the clamping part 42 to move to release the clamping with the reaction container 5, and meanwhile, the third driving part 11a drives the dispensing disc 6 to rotate, so that the reaction container 5 after the detection is finished continues to rotate to the next station along with the dispensing disc 6. Thereby, the positioning accuracy of the reaction container 5 at the detection station 1c is ensured by the auxiliary positioning of the positioning component 4, and the relative position of the container mouth and the through hole of the reaction container 5 is ensured not to deviate.

The dispensing disc 6 drives the reaction container 5 to move to the cup discarding station 1d, and when the cup discarding assembly 2 throws the reaction container 5 in place, the cup discarding method of the cup discarding assembly 2 specifically comprises the following steps: before the reaction vessel 5 is detected to move to the cup discarding station 1d, the second driving part 22 is controlled to move so as to drive the matching component 221 to move, and then the pushing part 23 is driven to move to the initial position along the first channel 213 towards the direction close to the dispensing tray 6, and when the reaction vessel 5 moves to the cup discarding station 1d, the second driving part 22 is controlled to move reversely, so that the pushing part 23 can be abutted with the reaction vessel 5 and drive the reaction vessel 5 to move along the first channel 213.

When the cup throwing is carried out through the cup throwing assembly 2, the position detection assembly 3 positioned on the cup throwing assembly 2 is at least used for detecting the position of the reaction container 5, so that the reaction container 5 is prevented from being blocked in a channel to influence the use of an incubation unit.

The working method specifically comprises the following steps: in the cup discarding station 1d, when the cup discarding assembly 2 pushes the reaction container 5 to move, if the reaction container 5 detected by the detecting portion 31 meets a preset condition, it is determined that the reaction container 5 is in a normal moving state in the cup discarding assembly 2. The cup discarding assembly 2 is combined with the position detecting mechanism 3, the motion of the reaction vessel 5 in the cup discarding assembly 2 is monitored by the detecting part 31, and if the detected reaction vessel 5 can be maintained under the condition that the detected reaction vessel 5 meets the preset condition all the time, the cup discarding station 1d is in a normal operation state. When the reaction vessel 5 detected by the detecting part 31 does not meet the preset condition, the position where the problem occurs can be judged according to the difference of the conditions of the specific condition which is not met and detected by the detecting part 31, so that the problem can be effectively solved in time, and the reaction vessel 5 is prevented from being blocked in the channel.

In one embodiment, the detecting portion 31 is located at a predetermined position in the first channel 213, and the cup discarding assembly 2 pushes the reaction vessel 5 to move to the predetermined position, so that the cup discarding operation is completed. The detection method comprises the following steps: in the cup discarding station 1d, in the process of pushing the reaction container 5 to move, if the reaction container 5 detected by the detection part 31 reaches a preset position at each preset time interval, the reaction container 5 is judged to be in a normal movement state in the cup discarding assembly 2, or if the number of times that the reaction container 5 detected by the detection part 31 passes through the preset position is preset, the reaction container 5 is judged to be in a normal movement state in the cup discarding assembly 2; otherwise, the first channel 213 is judged to be blocked by the clamping cup of the reaction vessel 5, and the detection part 31 sends out an alarm signal and controls to stop. The detection unit 31 provided at a predetermined position can detect and count the in-place condition of the reaction vessel 5 moving along the first passage 213.

The preset data set by the identification unit in the detection unit 31 is determined according to the specific conditions such as the rotation speed of the dispensing disc 6, the time required by the cup discarding assembly 2 when the reaction containers 5 are thrown, etc., and the preset data may be the preset times detected by the counter of the reaction containers in a period of time, or the preset time when the two adjacent reaction containers 5 reach the preset position is detected, and the preset time may be detected by the timer in the identification unit. Taking a preset number of times set in advance as an example, the number of times of counting the reaction containers 5 detected by the counter every 1 hour is set. When the cup discarding assembly 2 drives the reaction container 5 to reach the preset position for completing one cup discarding operation in 1 hour in the cup discarding process, the reaction container 5 can be detected by the detecting part 31 every time at the preset position and recorded as 1 time by the timer. After one hour, comparing the detection times calculated by the counter with preset times, if the detection times are equal to the preset times, the detection times represent that each cup throwing operation in the cup throwing assembly 2 is normal, and the detection in the next hour can be carried out again after the operation is continued. If the number of detections is less than the preset number, it indicates that there may be at least one time of cup throwing, where the reaction container 5 does not reach the preset position, and one or more subsequent reaction containers 5 are blocked in the channel. When the reaction vessel 5 blocked in the first channel 213 is removed by the worker, the detection unit 31 is restarted and repeats a new round of timing detection.

In an embodiment, the detecting portion 31 is disposed at one or more positions before the preset position along the extending direction of the first channel 213, and in the process of pushing the reaction container 5 to move at the cup discarding station 1d, if the detecting portion 31 can detect the reaction container 5 at the corresponding position and the detected same reaction container 5 is maintained at the detected position for less than the preset time, it is determined that the reaction container 5 is in a normal moving state in the cup discarding assembly 2, otherwise, it is determined that the first channel 213 is blocked by a cup of the reaction container 5, and the detecting portion 31 sends an alarm signal and controls to stop. Specifically, the timer set by the identification unit in the detecting portion 31 can time the time when the reaction container 5 is identified by the identification unit, if the cup discarding assembly 2 drives the reaction container 5 to move to realize normal operation of the cup throwing, the time when the reaction container 5 passes through the detecting portion 31 and is identified by the detecting portion can be set to be not longer than the preset time according to the moving speed of the reaction container 5 in the first channel 213. When the time of the reaction vessel 5 identified by the detection section 31 exceeds the preset time, it indicates that the reaction vessel 5 may be jammed in or has already been jammed to the position where the detection section 31 is provided, at least one reaction vessel 5 is jammed in the first passage 213. The detector 31 sends out an alarm signal to control the stop until the operator removes the reaction containers 5 accumulated in the first passage 213 and then restarts the reaction.

In an embodiment, the detecting portion 31 is further disposed in the housing 11 or the cup discarding assembly 2 corresponding to the cup discarding station 1d, for detecting the in-place rotation of the reaction container 5 to the cup discarding station 1 d. Before the reaction vessel 5 is pushed to move, the detection part 31 can detect the position where the dispensing tray 6 drives the reaction vessel 5 to move, and when the reaction vessel 5 is detected to move to the cup discarding station 1d, the detection part 31 can send an in-place signal to control the dispensing tray 6 to stop. The detector 31 serves as an in-place detector, and recognizes that the reaction container 5 reaches the cup discarding station 1d, and after in-place detection, can form an in-place signal to be sent to the third drive unit 11a and the second drive unit 22 of the cup discarding assembly 2. The third driving part 11a controls the dispensing disc 6 to stop moving after receiving the in-place signal, and the second driving part 22 starts the pushing part 23 at the initial position to start the cup throwing operation. During the process of moving the reaction vessel 5 along the first channel 213, the detection parts 31 arranged at different positions start to work, and if any detection part 31 positioned in the first channel 213 or at a preset position detects that a blocking condition exists according to the method, an alarm signal can be sent to control the whole cup throwing work or sample analysis work to stop until the fault is removed, and then the operation is restarted.

Alternatively, the detecting portions 31 may be further disposed on two sides of the pushing portion 23 along the moving direction of the pushing portion 23, so as to limit the movement of the pushing portion 23, and when the second driving portion 22 is controlled to move to drive the pushing portion 23 to move, the position and the timing of the movement may be set by the detecting portions 31 disposed on two sides of the pushing portion 23. When the pushing part 23 moves to the reaction container 5 to reach the preset position, the detection part 31 recognizes the position of the pushing part 23 to control the stopping, and after the detection part 31 detects the pushing part 23, the second driving part 22 is controlled to reversely move according to the preset time sequence to reset, after the pushing part 23 at the reset position is recognized by the corresponding detection part 31, a in-place signal is sent out, the second driving part 22 is controlled to stop moving according to the time sequence, and the third driving part 11a is started to enter the next reaction container 5 to enter the cup discarding station 1d and complete the cup discarding work, so that the cycle is completed.

Similarly, the detection portion 31 provided in the second channel 214 may also perform the position detection in the above manner, for example, in the process of pushing the reaction container 5 in the cup discarding station 1d, if the detection portion 31 can detect the reaction container 5 at the position corresponding to the second channel 214 and the detected same reaction container 5 is maintained at the detection position for less than the preset time, it is determined that the reaction container 5 is in the normal motion state in the cup discarding assembly 2, otherwise, it is determined that the second channel 214 has the reaction container 5 accumulated, and the detection portion 31 sends an alarm signal and controls to stop. In this way, the condition of the reaction vessel 5 in the second channel 214 is identified and detected, and the reaction vessel 5 of the collecting device is prevented from flowing back to the second channel 214 after being filled, so that the second channel 214 is prevented from being jammed. And will not be described in detail herein.

The embodiments of the present application have been described above, the foregoing description is exemplary, not exhaustive, and not limited to the embodiments disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the various embodiments described. The terminology used herein was chosen in order to best explain the principles of the embodiments, the practical application, or the improvement of technology in the marketplace, or to enable others of ordinary skill in the art to understand the embodiments disclosed herein.

Claims

1. An incubation unit for a sample analyzer, comprising:

The cup discarding station is provided with a cup discarding assembly and a position detecting assembly, the cup discarding assembly comprises a second driving part and a pushing part which are matched, the second driving part is used for driving the pushing part to move, when the reaction container moves to the cup discarding station, the pushing part is used for separating and pushing the reaction container from the dispensing tray to a preset position,

2. The incubation unit according to claim 1, wherein the cup-in station, the sample-adding mixing station, the detection station and the cup-discarding station are circumferentially arranged on the body in a time sequence, the body is provided with a containing cavity, the dispensing tray is rotatably connected with the containing cavity,

the dispensing tray is provided with a plurality of holding positions along the circumferential direction, and the holding positions are used for holding the reaction containers.

3. An incubation unit according to claim 2, wherein there is a fit gap between the reaction vessel and the receptacle,

the dispensing disc drives the reaction container to rotate to the detection station, and the clamping part moves to be clamped with the reaction container so as to limit the relative movement of the reaction container and the containing position.

4. The incubation unit according to claim 2, wherein the clamping portion comprises a clamping block provided with a clamping groove, the clamping block is connected with the output end of the first driving portion, and the clamping block can be driven to move along the radial direction of the body until the clamping groove abuts against the reaction container.

5. An incubation unit according to claim 2, wherein the cup reject assembly further comprises a mounting bracket provided with a first channel communicating with the receiving cavity corresponding to the cup reject station, the second drive section being mounted to the mounting bracket,

the second driving part comprises a driving component and a matching component, the pushing part is connected to the matching component, and the matching component is used for driving the pushing part to move so that the pushing part can push the reaction container to move to the preset position along the first channel.

6. An incubation unit according to claim 5, wherein the detection section is connected to at least the first channel,

the detection part can detect the position of the reaction container in the first channel when the pushing part pushes the reaction container to move along the first channel.

7. An incubation unit according to claim 6, wherein the detection means is capable of detecting whether the reaction vessel has reached or passed the predetermined position.

8. The incubation unit of any one of claims 1-4 wherein the body comprises:

a housing having an open end;

an incubation tray positioned within the housing, at least the positioning assembly being connected to the incubation tray;

a top cover covered on the opening end and detachably connected with the shell,

the top cover is provided with at least one through hole at a position corresponding to the sample adding and mixing station;

the heat preservation covers at least one side of the top cover, and the through holes penetrate through the heat preservation.

9. A sample analyser comprising an incubation unit according to any one of claims 1 to 8.

10. The utility model provides a sample analysis method for incubating the unit, its characterized in that, incubation unit includes body, divides annotate the dish, locating component, abandons a cup subassembly and position detection subassembly, divide annotate the dish and be used for driving place in divide the reaction vessel of annotating the dish and advance cup station, application of sample mixing station, detection station and abandon between the cup station, locating component includes first drive portion and joint portion, abandon a cup subassembly and include second drive portion and pushing away the portion, the position detection subassembly includes at least one detection portion, sample analysis method includes: