CN116406678A - Spray for blocking new coronavirus, preparation method and application thereof - Google Patents

Spray for blocking new coronavirus, preparation method and application thereof Download PDF

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Publication number
CN116406678A
CN116406678A CN202111665386.4A CN202111665386A CN116406678A CN 116406678 A CN116406678 A CN 116406678A CN 202111665386 A CN202111665386 A CN 202111665386A CN 116406678 A CN116406678 A CN 116406678A
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powder
spray
vitamin
eucommia ulmoides
weight
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何新桥
何山
何小珊
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Changde Jizhi Biotechnology Co ltd
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Changde Jizhi Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
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    • A—HUMAN NECESSITIES
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    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
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    • A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline 
    • A61K31/353—3,4-Dihydrobenzopyrans, e.g. chroman, catechin
    • A61K31/355—Tocopherols, e.g. vitamin E
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Abstract

The invention provides a spray for blocking new coronaviruses, a preparation method and application thereof, and relates to the technical field of biological medicines. The spray provided by the invention is prepared from the following raw materials: mucor powder, soybean protein powder, glucose powder, isomaltooligosaccharide, vitamin B2, vitamin E, fish skin peptide powder, eucommia ulmoides leaf juice and small molecular water. The spray prepared by the invention can safely and effectively block and inactivate the novel coronavirus, the inactivation rate reaches more than 99 percent, and the action time is long. In addition, the preparation method provided by the invention is simple and easy to operate, and is suitable for industrial mass production.

Description

Spray for blocking new coronavirus, preparation method and application thereof
Technical field:
the invention relates to the technical field of biological medicine, in particular to a spray for blocking new coronaviruses, a preparation method and application thereof.
The background technology is as follows:
the spray and the application of the spray are active protection products for blocking new coronaviruses, the design concept of the spray and the application of the spray is derived from human vaccines, the names are different, the purpose of preventing viruses is the same, and the spray and the application of the spray are innovations based on the vaccines. Vaccine discovery is a milestone-like event in the history of human development. In a sense, the history of human reproduction is the history of continuous disease and natural disaster fight of humans, the most important means of controlling infectious diseases is prevention, and vaccination is considered the most effective means. The fact proves that the smallpox virus threatening human beings for hundreds of years is thoroughly destroyed after the occurrence of vaccinia epidemic, and the first victory of human beings against the virus with the vaccine is more convinced that the vaccine has the effect of controlling and eliminating infectious diseases. The vaccine family has been expanding and developed over 200 years later, and more than 20 vaccines for preventing and treating human diseases are currently available.
It is known that vaccination has a major role in preventing human infection with virus, requiring clinic and long time, and large development cost, and that vaccines make a great contribution to human health, but vaccines need to be injected into human body, there are more or less uncertain factors and potential safety hazards, such as new coronavirus vaccines worldwide, the biggest pharmaceutical companies worldwide, developed new coronavirus vaccines have been used in batches, but allergy and side effects of new coronavirus vaccines in experiments, and immune deficiency people such as children, cancer patients, organ transplantation, pregnant women, aids patients, whether or not they can be vaccinated, which people can meet seeds, and the like, become problems and choices faced by people. The effective time of the vaccine, potential risk, unknown side effects, etc., require a significant amount of time and time for human use to find an answer. In addition, the production time varies from vaccine to vaccine, and some vaccines even require approximately 2 years to produce a batch. Vaccine development is a lengthy and complex process and is costly. The speed of vaccine development never goes up to the speed of virus variation.
It is well known that all viruses are derived from the environment where the nature exists, and human vaccines are developed after the viruses are found, and the viruses belong to passive prevention, and are difficult to treat both symptoms and root causes. The spraying and applying spray is to spray in public places and living rooms in advance when viruses are not found, so that the viruses can be driven and effectively inactivated, the viruses are hidden everywhere, and the method belongs to active prevention. The spray does not need to be injected into a human body, only needs to treat the indoor environment and is stuck to a certain part of the body, integrates the effectiveness, the safety, the timeliness and the flexibility, and can make up the defects of various uncertain factors of human vaccines.
The invention comprises the following steps:
the invention aims to provide a spray for blocking new coronaviruses, a preparation method and application thereof. The spray prepared by the invention can safely and effectively block and inactivate the novel coronavirus, and has long action time.
The invention provides a spray for blocking new coronaviruses, which is prepared from the following raw materials: 0.1-0.5kg of mucor powder, 70-100kg of soybean protein powder, 100-200kg of glucose powder, 1-2kg of isomaltooligosaccharide, 0.1-0.3kg of vitamin B, 0.05-0.2kg of vitamin E, 0.05-0.3kg of fishskin peptide powder, 0.1-0.5kg of eucommia ulmoides leaf juice and 50-60kg of micromolecular water.
Preferably, the weight ratio of the mucorales powder to the vitamin E is 2-6:1; more preferably, the weight ratio of mucorales powder to vitamin E is 4:1.
Preferably, the spray is prepared from the following raw materials: mucor powder 0.4kg, soybean protein powder 80kg, glucose powder 150kg, isomaltooligosaccharide 1.6kg, vitamin B2.2 kg, vitamin E0.1 kg, fish skin peptide powder 0.22kg, eucommia ulmoides leaf juice 0.4kg, and small molecule water 58kg.
The invention also provides a preparation method of the spray, which comprises the following steps:
(1) Taking soybean protein powder according to the weight, steaming, and naturally cooling to room temperature; taking fresh eucommia ulmoides leaves, cleaning, crushing and juicing to obtain eucommia ulmoides leaf juice for later use;
(2) Adding mucor powder into the soybean protein powder obtained in the step (1) according to the weight, uniformly stirring, naturally fermenting for 7 days, crushing into slurry, and filtering;
(3) Taking eucommia ulmoides leaf juice according to the weight, adding the eucommia ulmoides leaf juice into the filtered slurry obtained in the step (2), naturally fermenting for 7 days again, and extracting and purifying at normal temperature to obtain fermentation liquor;
(4) Mixing glucose powder, vitamin B2, vitamin E, isomaltooligosaccharide and fish skin peptide powder according to weight, grinding for 6-8h to 20-30nm to obtain mixed powder;
(5) Taking small molecular water according to the weight, adding the mixed powder obtained in the step (4) into the small molecular water, uniformly mixing, adding the fermentation broth obtained in the step (3), stirring, uniformly mixing, and filtering to obtain filtrate, namely the spray for blocking the new coronavirus.
In another aspect, the invention also provides the use of the spray described above for blocking new coronavirus products. Applications of the sprays for blocking new coronaviruses described herein include, but are not limited to, being formulated as sprays or applications.
The application method and mechanism are as follows:
1. the spray for blocking the new coronavirus is made into spray, and is sprayed on clothes, and the mucorales catch the virus in the air, so that the virus protein is rapidly dissolved, the probability of human body infection of the new coronavirus is reduced, the carrying is convenient, and the active protection is realized; the novel coronavirus and other viruses in the air can be captured by being sprayed in vehicles, indoor wall surfaces, schools, hotels, stations, airports and other public places; can also be added in an ultrasonic atomizer for atomization, and is conveyed to public places through a central air conditioner and an air supply pipe of a fresh air system for inactivating viruses.
2. The spray for blocking the new coronavirus is added into a nontoxic water-absorbing resin material, and is made into an application by using a breathable non-woven fabric material and breathable glue, and is attached to a certain part of a body, and nanoscale active mucorales can enter the body through capillary holes to inactivate the new coronavirus in the body and the body.
The invention has the beneficial effects that:
(1) The spray prepared by the method is a nano solvent, has biological activity, can rapidly dissolve viral proteins, rapidly inactivate the novel coronavirus, is harmless to human bodies, and has an inactivation rate of more than 99%; in addition, mucor in the raw materials exists in natural environment and indoor air, and the protein in the prepared spray, application and other products is a temperature bed for growth and propagation of mucor, so that the effective action time of the product is longer.
(2) The invention also discovers that the effect and the action time of inactivating influenza viruses can be obviously enhanced by adjusting the proportion of mucormycosis powder and vitamin E. In addition, the preparation method provided by the invention is simple and easy to operate, and is suitable for industrial mass production.
Detailed Description
In order that the manner in which the above recited features, advantages, objects and advantages of the present invention are obtained will become readily apparent, a more particular description of the invention will be rendered by reference to specific embodiments thereof which are illustrated in the appended drawings. Based on the examples in the embodiments, those skilled in the art can obtain other examples without making any inventive effort, which fall within the scope of the invention.
The experimental methods in the following examples are conventional methods unless otherwise specified, and materials, reagents, etc. used in the following examples are commercially available unless otherwise specified.
Example 1
A spray for blocking new coronavirus and its preparation method are provided:
a spray for blocking new coronaviruses is prepared from the following raw materials: mucor powder 0.1kg, soybean protein powder 70kg, glucose powder 100kg, isomaltooligosaccharide 1kg, vitamin B2.1 kg, vitamin E0.05 kg, fish skin peptide powder 0.05kg, eucommia ulmoides leaf juice 0.1kg and small molecular water 50kg.
The preparation method of the spray comprises the following steps:
1) Taking soybean protein powder according to the weight, steaming, and naturally cooling to room temperature; taking fresh eucommia ulmoides leaves, cleaning, crushing and juicing to obtain eucommia ulmoides leaf juice for later use;
(2) Adding mucor powder into the soybean protein powder obtained in the step (1) according to the weight, uniformly stirring, naturally fermenting for 7 days, crushing into slurry, and filtering;
(3) Taking eucommia ulmoides leaf juice according to the weight, adding the eucommia ulmoides leaf juice into the filtered slurry obtained in the step (2), naturally fermenting for 7 days again, and extracting and purifying at normal temperature to obtain fermentation liquor;
(4) Mixing glucose powder, vitamin B2, vitamin E, isomaltooligosaccharide and fish skin peptide powder according to weight, grinding for 6 hours to 30nm to obtain mixed powder;
(5) Taking small molecular water according to the weight, adding the mixed powder obtained in the step (4) into the small molecular water, uniformly mixing, adding the fermentation broth obtained in the step (3), stirring, uniformly mixing, and filtering to obtain filtrate, namely the spray for blocking the new coronavirus.
Example 2
A spray for blocking new coronavirus and its preparation method are provided:
a spray for blocking new coronaviruses is prepared from the following raw materials: mucor powder 0.4kg, soybean protein powder 80kg, glucose powder 150kg, isomaltooligosaccharide 1.6kg, vitamin B2.2 kg, vitamin E0.1 kg, fish skin peptide powder 0.22kg, eucommia ulmoides leaf juice 0.4kg and small molecule water 58kg. Namely the weight ratio of the mucorales powder to the vitamin E is 4:1.
The preparation method of the spray comprises the following steps:
1) Taking soybean protein powder according to the weight, steaming, and naturally cooling to room temperature; taking fresh eucommia ulmoides leaves, cleaning, crushing and juicing to obtain eucommia ulmoides leaf juice for later use;
(2) Adding mucor powder into the soybean protein powder obtained in the step (1) according to the weight, uniformly stirring, naturally fermenting for 7 days, crushing into slurry, and filtering;
(3) Taking eucommia ulmoides leaf juice according to the weight, adding the eucommia ulmoides leaf juice into the filtered slurry obtained in the step (2), naturally fermenting for 7 days again, and extracting and purifying at normal temperature to obtain fermentation liquor;
(4) Mixing glucose powder, vitamin B2, vitamin E, isomaltooligosaccharide and fish skin peptide powder according to weight, grinding for 8 hours to 20nm to obtain mixed powder;
(5) Taking small molecular water according to the weight, adding the mixed powder obtained in the step (4) into the small molecular water, uniformly mixing, adding the fermentation broth obtained in the step (3), stirring, uniformly mixing, and filtering to obtain filtrate, namely the spray for blocking the new coronavirus.
Example 3
A spray for blocking new coronavirus and its preparation method are provided:
a spray for blocking new coronaviruses is prepared from the following raw materials: mucor powder 0.5kg, soybean protein powder 100kg, glucose powder 200kg, isomaltooligosaccharide 2kg, vitamin B2.3 kg, vitamin E0.2 kg, fish skin peptide powder 0.3kg, eucommia ulmoides leaf juice 0.5kg and small molecular water 60kg.
The preparation method of the spray comprises the following steps:
1) Taking soybean protein powder according to the weight, steaming, and naturally cooling to room temperature; taking fresh eucommia ulmoides leaves, cleaning, crushing and juicing to obtain eucommia ulmoides leaf juice for later use;
(2) Adding mucor powder into the soybean protein powder obtained in the step (1) according to the weight, uniformly stirring, naturally fermenting for 7 days, crushing into slurry, and filtering;
(3) Taking eucommia ulmoides leaf juice according to the weight, adding the eucommia ulmoides leaf juice into the filtered slurry obtained in the step (2), naturally fermenting for 7 days again, and extracting and purifying at normal temperature to obtain fermentation liquor;
(4) Mixing glucose powder, vitamin B2, vitamin E, isomaltooligosaccharide and fish skin peptide powder according to weight, grinding for 7 hours to 25nm to obtain mixed powder;
(5) Taking small molecular water according to the weight, adding the mixed powder obtained in the step (4) into the small molecular water, uniformly mixing, adding the fermentation broth obtained in the step (3), stirring, uniformly mixing, and filtering to obtain filtrate, namely the spray for blocking the new coronavirus.
Example 4
A spray for blocking new coronavirus and its preparation method are provided:
the difference from example 2 is only that the weight ratio of mucor powder to vitamin E is 2:1, i.e. the mucor powder is 0.3kg and the vitamin E is 0.15 kg.
Example 5
A spray for blocking new coronavirus and its preparation method are provided:
the difference from example 2 is only that the weight ratio of mucor powder to vitamin E is 6:1, wherein the weight ratio of mucor powder to vitamin E is 0.48kg and the weight ratio of vitamin E is 0.08 kg.
Comparative example 1
A spray for blocking new coronavirus and its preparation method are provided:
the only difference from example 2 is that no mucor powder was added.
Comparative example 2
A spray for blocking new coronavirus and its preparation method are provided:
the only difference from example 2 is that no vitamin E was added.
Test example 1
The sprays prepared in examples 1-5 and comparative examples 1-2 were subjected to virus inactivation tests.
The test method refers to the virus inactivation test of the 2020 edition-2.1.1.10 of the disinfection technical Specification;
test virus: unmutated new coronavirus strain, new coronavirus D614G variant strain, new coronavirus N501Y variant strain, host cell: vero E6 cells;
test drug concentration: 100 μg/ml, solvent is normal saline; the action time is 2h.
The test results are shown in tables 1-3:
table 1: inhibition of unmutated novel coronaviruses by the present invention
Figure RE-RE-GDA0003884547270000051
Table 2: the invention has the inhibition effect on the novel coronavirus D614G mutant virus
Figure RE-RE-GDA0003884547270000052
Figure RE-RE-GDA0003884547270000061
Table 3: the invention has the inhibition effect on the N501Y mutant virus of the novel coronavirus
Figure RE-RE-GDA0003884547270000062
Conclusion of the test: the experiment shows that the spray for blocking the new coronavirus obtained by the preparation method provided by the invention can effectively inactivate the new coronavirus, the inactivation rate reaches more than 99%, and cells are not damaged; comparing examples 2,4 and 5 with comparative examples 1,2, it was found that the effect of inactivating chronic influenza virus can be remarkably enhanced by adjusting the ratio of mucorales powder and vitamin E, which have a synergistic effect.
It is to be understood that the above examples are provided for the purpose of clearly illustrating the invention and are not to be construed as limiting the invention in any way. It is intended to enable one skilled in the art to understand the present invention and to practice it accordingly, and is not intended to limit the scope of the present invention. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention as set forth in the claims.

Claims (6)

1. The spray for blocking the novel coronavirus is characterized by being prepared from the following raw materials: mucor powder 0.1-0.5kg, soybean protein powder 70-100kg, glucose powder 100-200kg, isomaltooligosaccharide 1-2kg, vitamin B2.1-0.3 kg, vitamin E0.05-0.2 kg, fish skin peptide powder 0.05-0.3kg, eucommia ulmoides leaf juice 0.1-0.5kg, and small molecular water 50-60kg.
2. The spray of claim 1, wherein the weight ratio of mucorales powder to vitamin E is 2-6:1.
3. The spray of claim 1, wherein the spray is prepared from the following raw materials: mucor powder 0.4kg, soybean protein powder 80kg, glucose powder 150kg, isomaltooligosaccharide 1.6kg, vitamin B2.2 kg, vitamin E0.1 kg, fish skin peptide powder 0.22kg, eucommia ulmoides leaf juice 0.4kg and small molecule water 58kg.
4. The method for preparing the spray according to claim 1, wherein the method comprises the following steps:
(1) Taking soybean protein powder according to the weight, steaming, and naturally cooling to room temperature; taking fresh eucommia ulmoides leaves, cleaning, crushing and juicing to obtain eucommia ulmoides leaf juice for later use;
(2) Adding mucor powder into the soybean protein powder obtained in the step (1) according to the weight, uniformly stirring, naturally fermenting for 7 days, crushing into slurry, and filtering;
(3) Taking eucommia ulmoides leaf juice according to the weight, adding the eucommia ulmoides leaf juice into the filtered slurry obtained in the step (2), naturally fermenting for 7 days again, and extracting and purifying at normal temperature to obtain fermentation liquor;
(4) Mixing glucose powder, vitamin B2, vitamin E, isomaltooligosaccharide and fish skin peptide powder according to weight, grinding for 6-8h to 20-30nm to obtain mixed powder;
(5) Taking small molecular water according to the weight, adding the mixed powder obtained in the step (4) into the small molecular water, uniformly mixing, adding the fermentation broth obtained in the step (3), stirring, uniformly mixing, and filtering to obtain filtrate, namely the spray for blocking the new coronavirus.
5. Use of a spray according to any one of claims 1-3 for blocking a new coronavirus product.
6. The use according to claim 5, wherein the spray is formulated as a spray or an application.
CN202111665386.4A 2021-12-31 2021-12-31 Spray for blocking new coronavirus, preparation method and application thereof Pending CN116406678A (en)

Priority Applications (1)

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CN202111665386.4A CN116406678A (en) 2021-12-31 2021-12-31 Spray for blocking new coronavirus, preparation method and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111665386.4A CN116406678A (en) 2021-12-31 2021-12-31 Spray for blocking new coronavirus, preparation method and application thereof

Publications (1)

Publication Number Publication Date
CN116406678A true CN116406678A (en) 2023-07-11

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202111665386.4A Pending CN116406678A (en) 2021-12-31 2021-12-31 Spray for blocking new coronavirus, preparation method and application thereof

Country Status (1)

Country Link
CN (1) CN116406678A (en)

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