CN116376911B - Plant drought, low temperature and osmotic stress induced promoter and application thereof - Google Patents

Plant drought, low temperature and osmotic stress induced promoter and application thereof Download PDF

Info

Publication number
CN116376911B
CN116376911B CN202310070086.4A CN202310070086A CN116376911B CN 116376911 B CN116376911 B CN 116376911B CN 202310070086 A CN202310070086 A CN 202310070086A CN 116376911 B CN116376911 B CN 116376911B
Authority
CN
China
Prior art keywords
promoter
primer
seq
plants
low temperature
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202310070086.4A
Other languages
Chinese (zh)
Other versions
CN116376911A (en
Inventor
解敏敏
龚达平
孙晋浩
陈明丽
李志远
李尊强
向小华
宗鹏
奥罗尔·里奇尔
屈旭
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qingzhou Tobacco Research Institute of China National Tobacco Corp of Institute of Tobacco Research of CAAS
Original Assignee
Qingzhou Tobacco Research Institute of China National Tobacco Corp of Institute of Tobacco Research of CAAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qingzhou Tobacco Research Institute of China National Tobacco Corp of Institute of Tobacco Research of CAAS filed Critical Qingzhou Tobacco Research Institute of China National Tobacco Corp of Institute of Tobacco Research of CAAS
Priority to CN202310070086.4A priority Critical patent/CN116376911B/en
Publication of CN116376911A publication Critical patent/CN116376911A/en
Application granted granted Critical
Publication of CN116376911B publication Critical patent/CN116376911B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/34—Vector systems having a special element relevant for transcription being a transcription initiation element

Abstract

The invention relates to the fields of biotechnology and plant genetic engineering, in particular to a promoter for drought, low temperature and osmotic stress induction of plants and application thereof. The nucleic acid sequence of the promoter is a DNA sequence shown as SEQ ID NO.1. The promoter can drive the high expression of an Arabidopsis thaliana C2H 2-type zinc finger transcription factor protein 5 (AtZAT 5) gene under drought and low temperature stress, can be used for improving and improving the growth characteristics and stress tolerance of plants, and has good application prospects in stress-tolerant genetic improvement of plants.

Description

Plant drought, low temperature and osmotic stress induced promoter and application thereof
Technical Field
The invention relates to the fields of biotechnology and plant genetic engineering, in particular to a promoter for drought, low temperature and osmotic stress induction of plants and application thereof.
Background
The promoter is a DNA sequence which is positioned in the upstream region of the 5' end of the gene, can bind RNA polymerase and ensure the accurate and effective initiation of gene transcription, and plays an important role in the expression regulation of the gene. The activity of a promoter determines the expression efficiency of an endogenous or exogenous gene. Three general categories can be distinguished based on the expression characteristics of the promoters: constitutive promoters, tissue-specific promoters, and inducible promoters. The constitutive promoter can effectively drive the excessive expression of exogenous genes, but can cause resource waste, even influence the growth and development of plants and normal physiological metabolism, for example, dwarf deformity of plants can be caused when the CaMV35S promoter is used for improving the stress resistance of plants. And the inducible promoters such as the adversity stress inducible promoters do not affect the growth metabolism of plants under normal conditions, and only when the plants are subjected to external stress, the over-expression of the adversity resistance genes is started, so that the plants show a certain tolerance or adversity resistance. Therefore, isolation and identification of stress-inducible promoters in plants and utilization thereof has become one of the research hotspots of plant genetic engineering in recent years.
Adverse stress such as drought, low temperature, high osmotic pressure and the like seriously affects quality and yield of crops. The transcription factors are driven by the stress-induced promoter, so that the expression of a plurality of stress-induced genes is activated, and the method is an effective means for improving the stress resistance of crops. Most of the currently reported adversity-induced promoters are induced by single adversity stress, but the researches on the promoters induced by multiple adversity stresses are relatively few. Because the plant is not only stressed by a single adversity, but also stressed by multiple adversity in different growth periods in the whole growth process of the plant, the single adversity stress inducible promoter shows a certain limitation in application, and the multiple adversity stress inducible promoters have more advantages in application of improving the comprehensive adversity resistance of the plant.
Chinese patent CN107099535a discloses a promoter induced by low temperature, high salt, drought or ABA, which can be induced and started by various adversity factors of low temperature, high salt, drought or ABA; chinese patent CN114921466A discloses an inducible promoter pDI19-1, and an expression vector construction method and application thereof, which can obviously improve the expression level of a target gene under the conditions of low temperature, drought, ABA (abscisic acid) and MeJA (methyl jasmonate). However, only these promoters can not meet the needs of research and application, and the invention aims to provide a novel plant drought, low temperature and osmotic stress induced promoter so as to enrich the variety of the promoters and meet the needs of application.
Disclosure of Invention
The invention aims to solve the technical problems that the number of the promoters obtained by current research is small, and the requirements of research and application cannot be met.
In order to solve the problems, the invention discovers a promoter induced by various stress through long-term research, in particular to a promoter ProAtZAT5 which is derived from Arabidopsis and is induced by drought, low temperature and high osmotic stress, and has good application prospect in genetic engineering improvement and genetic improvement of comprehensive stress resistance of plants.
In order to achieve the above purpose, the invention is mainly realized by the following technical means: a promoter for drought, low-temp and osmotic stress induction of plant has the nucleic acid sequence of SEQ ID NO.1.
SEQ ID NO.1：
aagtgatatgattatcatactaatatttagaagtggttaaaaataaatatattttattattattcttctttctttttcttttctttttttttgacagggatttttgttttttaaaaagagtccttgacaaggattttgtggtgtggtttagccactatgcattataccttatgcatagatatgggttcaagcctcaatctcccactgtgagactttgaaagtatgttgacaaaaaaaggtgtataatttttccagaataaattattttaatctagtttgcctagctaaattaaataattattttgaggtccctaaacttcataattttccggtctctcaatcttgacataacttattctctgtctatagtttttttttttttgaacttttctctatagattacttattctataatctataaagtattatttttggatgtaccgtgtaaactcgttttaatggttagttgtcacttgtccatacaaaaatgtaacattccaatatttgacaataaagtaatgttacataaaaaatttctataatttgagtatgttttttttttttttttgtcggcctataatttgagtatgttaattaatctcaattgtaaaaatagatctctaattatcttaaaactgtttccagtcttaagattagtagataaccaattgagcactagctttcaactctctctacaaatgttaatcaagatatgattaaaaaatgagagaaaaaaaaaagtcgtggtgttgtctcttcacattaacaaagactaagcttaatattgtttttagcctaacatgccaccgatgaatgcacctaatcctataaacaccggccactgcatgtcgcctcctaattatgaaccatgtatgtctgtgaatcattatttccctttgataatcttattagtatatttcaaaacatttaatcaaaagttttcttgtagtcgtcacgaccattaataatatgtaatatattctttttatttgctgtcgtgaacatttttatctttagttctatctatttcggttctcattgaaagcgtcgatttgatgacgtggacctaaagtgcgtgaaagtaaaacctttgctttgtccttagaactgaatgttcacactcccaaaaattaaattcgaaactaaataaataaaaacaaataacaaaaacgtgaattggtggcctaagtgccactcaacactactaatgaacctttgcctaattcagtggttaactcttcacgggtttcactttcccttttatttctcctacttttgcttgaaacaatattaatcattttcactcggaattaataacaattctctgttaattatggaatatgaatgattcaagagtttgattaatcacaccaattgatattaatatgtttgttattagaaagtatgtactagaagaagctgtctaatacaatactaataaatgaaaaaacgtcatgaagggttgacttctggtatatagatctaggtagagatcagattttgaagatatggattaaaagtagatacaacagaacagaaactagaaaagaagaaaaattatttacatgtcatatttttttcatcattgaattataaatttgttgattgattttattgttatggaatttggcagagaaaacgcagaagtgtcaggtaatgggtatagagtgaaccgtcaaaatgttattggttagctaaaaacattataataatgtaaagtggatcgatgtgtaaaatcttttgtggagtaaactaaaaagattaacaaccaccaaacttataacctctctctttttttttccgataaatatatatataagcagccccaccatcgtcttcttcactgcctctcttctctcttctcttctgtctctctcttgttagttcactccacataataaacaccaaagatttcattctcttctccataatttcgaagtttcttgaattgggtttgtttcttgatttgtttcttgaattgggttttggtcttcttttcttactatatttggat
The promoter disclosed by the invention can drive the high expression of an Arabidopsis C2H 2-type zinc finger transcription factor protein 5 (AtZAT 5) gene under drought and low-temperature stress, so that the promoter is named as ProAtZAT5. The promoter is a promoter of a section of gene obtained by cloning from an arabidopsis genome, then a recombinant vector for driving the expression of a reporter gene beta-glucosidase Gene (GUS) by the promoter is constructed, the constructed recombinant vector is transformed into arabidopsis, and a single copy inserted transgenic homozygous strain is obtained through multi-generation resistance screening; GUS histochemical staining experiments are carried out on the obtained transgenic homozygous plants, and the GUS histochemical staining experiments show that the promoter drives GUS reporter genes to be expressed at high level under drought, cold and high osmotic stress induction.
A primer pair for cloning the promoter ProAtZAT5, which comprises a first primer and a second primer, wherein the DNA sequence of the first primer is shown as SEQ ID NO. 2; the DNA sequence of the second primer is shown as SEQ ID NO. 3.
SEQ ID NO.2：AAGTGATATGATTATCATAC
SEQ ID NO.3：ATCCAAAATATAGTAAGAAAAG
A primer pair for amplifying the promoter ProAtZAT5 comprises a first primer and a second primer, both of which contain homologous arm sequences of enzyme cutting sites, and amplification products can be connected into a plant expression vector in a homologous recombination mode. The DNA sequence of the first primer is shown as SEQ ID NO. 4; the DNA sequence of the second primer is shown as SEQ ID NO. 5.
SEQ ID NO.4：caagcttggctgcaggtcgacAAGTGATATGATTATCATAC
SEQ ID NO.5：tcttagaattcccggggatccATCCAAAATATAGTAAGAAAAG
An inducible promoter eukaryotic expression vector for plant transformation, which comprises a promoter with a nucleotide sequence of SEQ ID NO.1, and further comprises pCAMBIA1391-ProAtZAT 5; the expression vector is recombinant expression vector, and can make the exogenous target gene express in high efficiency. The method is realized by the following steps: the recombinant expression vector is used for transforming plants, and the obtained transformed plants can induce the efficient expression of exogenous genes through drought and/or low-temperature treatment.
A transformant which is a plant expression vector, a host into which a promoter having a nucleotide sequence of SEQ ID NO.1 is introduced or a host into which the above recombinant eukaryotic expression vector is introduced. Further, the transformant is a cell line or a callus or a transgenic plant. The host at the time of transformation is Agrobacterium.
The promoter ProAtZAT5 is applied to plant stress resistance gene research or stress resistance genetic improvement. The specific method is to transform plants with a recombinant expression vector containing the promoter, which is used for improving tolerance or stress resistance of the plants or treat the obtained transformed plants through drought and/or low temperature and/or osmotic stress, and is used for inducing efficient expression of exogenous genes.
The invention has the beneficial effects that:
the promoter provided by the invention specifically drives exogenous genes to express in plants under drought, low temperature and osmotic stress, so that the promoter can be used for improving and improving the growth characteristics and stress tolerance of plants, and has good application prospects in stress-tolerant genetic improvement of plants.
Drawings
Fig. 1: the promoter ProAtZAT5PCR amplification electrophoretogram of the invention;
fig. 2: plasmid PCR electrophoresis diagram of the promoter ProAtZAT5 connection expression vector pCAMBIA 1391;
fig. 3: the plant expression vector pCAMBIA1391-ProAtZAT5 constructed by the invention is shown in GUS schematic diagram;
fig. 4: in the invention, the ProAtZAT5 drives GUS gene to carry out GUS histochemical staining under drought and low temperature stress conditions.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. It will be apparent that the described embodiments are some, but not all, embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1: cloning of promoters
1.1 preparation of Arabidopsis genome DNA
Taking arabidopsis planted in a greenhouse, taking tender leaves of the arabidopsis, extracting genome DNA by using a plant genome DNA extraction kit, detecting by agarose gel electrophoresis, and preserving at-20 ℃ for later use.
1.2 cloning of promoter ProAtZAT5
The arabidopsis genome DNA is taken as a template, and the upstream primer is as follows: AAGTGATATGATTATCATAC (SEQ ID NO. 2), the downstream primer is: ATCCAAAATATAGTAAGAAAAG (SEQ ID NO. 3) the ProAtZAT5 promoter candidate region was obtained by PCR amplification under the following conditions: pre-denaturation at 95℃for 3min, denaturation at 95℃for 15min, annealing at 50℃for 15s, extension at 72℃for 2min, 30 cycles total, and storage at 4 ℃. The PCR product was electrophoretically detected (FIG. 1) and sequenced to obtain the 2014bp ProAtZAT5 promoter, which was SEQ ID NO.1.
Example 2: construction of plant expression vectors
The pCAMBIA1391 vector was digested with SalI/BamHI, and the large fragment was recovered for use. The PCR was again performed using the PCR product of example 1 as a template and caagcttggctgcaggtcgacAAGTGATATGATTATCATAC (SEQ ID NO. 4) and tcttagaattcccggggatccATCCAAAATATAGTAAGAAAAG (SEQ ID NO. 5) under the same amplification conditions as in example 1. The PCR product was then purified and recovered, and the promoter-order fragment was ligated into pCAMBIA1391 vector by means of homologous recombination, and positive recombinants were determined by PCR sequencing identification (FIG. 2). Thus, a plant recombinant expression vector pCAMBIA1391-ProAtZAT5:: GUS (FIG. 3) capable of expressing GUS gene was obtained.
Example 3: GUS Activity assay
3.1 Agrobacterium-mediated plant transformation
GUS was transformed into GV3301 in Agrobacterium by electric shock transformation.
Taking Arabidopsis seeds, sterilizing by sodium hypochlorite, sowing the Arabidopsis seeds on a seedling culture medium, and transferring the Arabidopsis seeds into soil after one week. After flowering, the inflorescence infection method is adopted, and the agrobacterium transferred into pCAMBIA1391-ProAtZAT 5:GUS is used for infection. Specifically, agrobacterium cultured at 28 ℃ is resuspended by using 5% sucrose solution after being cultured to OD600 = 0.8, and infection transformation is carried out by a flower dipping mode, and plants grow normally in a tissue incubator (24 ℃ for 16h/8h day/night cycle) after infection. After harvesting the seeds, resistance selection was performed on hygromycin medium containing 15 mg/L.
3.2 analysis of GUS expression Activity in transgenic plants
Seedlings of transgenic Arabidopsis T3 generation homozygous material were planted for GUS histochemical staining analysis, and fluorescence detection of GUS activity was performed in accordance with Jefferson method. Seedlings grown in soil for about 2 weeks are respectively subjected to stress treatment and drought treatment: placing the seedlings on a culture dish for water shortage for 8 hours, and then rehydrating overnight; high-permeability treatment of PEG 6000: the seedlings were placed in petri dishes containing 25% peg6000 in water overnight; and (3) low-temperature treatment: the seedlings were placed in an aqueous petri dish for overnight treatment at 4 ℃; control: seedlings were placed in an aqueous petri dish and cultured under normal conditions. The control and stress treated seedlings were then immersed in GUS staining solution, evacuated overnight at 37℃and decolorized with 75% ethanol, the staining observed under an inverted microscope, and photographed for recording.
The results showed that promoter proatat 5 was able to drive strong high level expression of GUS gene in transgenic plants under drought, low temperature, PEG6000 stress conditions (fig. 4).
The foregoing description of the preferred embodiments of the present invention is provided for the purpose of illustration only, and is not intended to limit the invention to the particular embodiments disclosed, but on the contrary, the intention is to cover all modifications, alternatives, and alternatives falling within the spirit and scope of the invention.

Claims (8)

1. The application of a promoter in drought resistance, low temperature and osmotic stress resistance gene research or genetic improvement of plants is provided, and the nucleic acid sequence of the promoter is a DNA sequence shown as SEQ ID NO.1.
2. Use of a primer pair for cloning the promoter of claim 1 in drought, low temperature, osmotic stress gene research or genetic improvement of plants, characterized in that: the primer pair comprises a first primer and a second primer, and the DNA sequence of the first primer is shown as SEQ ID NO. 2; the DNA sequence of the second primer is shown as SEQ ID NO. 3.
3. Use of a primer pair for amplifying the promoter of claim 1 in drought, low temperature, osmotic stress gene research or genetic improvement of plants, characterized in that: the primer pair comprises a first primer and a second primer, and the DNA sequence of the first primer is shown as SEQ ID NO. 4; the DNA sequence of the second primer is shown as SEQ ID NO. 5.
4. The use according to claim 1, wherein: when in use, a recombinant expression vector containing a promoter with a nucleotide sequence of SEQ ID NO.1 is constructed.
5. The use according to claim 1, wherein: when in use, a transformant which is introduced with a promoter with a nucleotide sequence of SEQ ID NO.1 is constructed.
6. The transformant according to claim 5, wherein: the transformant is a cell line or a callus or a transgenic plant.
7. The transformant according to claim 5, wherein: the host at the time of transformation is Agrobacterium.
8. The use according to claim 1, wherein: transforming plants with a recombinant expression vector comprising said promoter, and using the obtained transformed plants under drought and/or low temperature and/or osmotic conditions for inducing efficient expression of exogenous genes.
CN202310070086.4A 2023-02-07 2023-02-07 Plant drought, low temperature and osmotic stress induced promoter and application thereof Active CN116376911B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202310070086.4A CN116376911B (en) 2023-02-07 2023-02-07 Plant drought, low temperature and osmotic stress induced promoter and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202310070086.4A CN116376911B (en) 2023-02-07 2023-02-07 Plant drought, low temperature and osmotic stress induced promoter and application thereof

Publications (2)

Publication Number Publication Date
CN116376911A CN116376911A (en) 2023-07-04
CN116376911B true CN116376911B (en) 2024-01-23

Family

ID=86964531

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202310070086.4A Active CN116376911B (en) 2023-02-07 2023-02-07 Plant drought, low temperature and osmotic stress induced promoter and application thereof

Country Status (1)

Country Link
CN (1) CN116376911B (en)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7186563B2 (en) * 2004-03-29 2007-03-06 Purdue Research Foundation Methods and compositions for regulating plant stress tolerance
US20150067923A1 (en) * 2013-08-13 2015-03-05 New York University Transgenic Plants And A Transient Transformation System For Genome-Wide Transcription Factor Target Discovery

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Lin,X等.Arabidopsis thaliana C2H2-type zinc finger family protein (AT2G28200), mRNA.NCBI Genbank.2022,第1-2页. *
Overexpression of MdZAT5, an C2H2-Type Zinc Finger Protein, Regulates Anthocyanin Accumulation and Salt Stress Response in Apple Calli and Arabidopsis;Da-Ru Wang等;Int J Mol Sci;第23卷(第3期);第1-16页 *
大豆两个C2H2型转录因子基因序列特征及表达分析;侯思宇等;植物生理学报;第665-674页 *
烟草C2H2锌指蛋白转录因子家族成员的鉴定与表达分析;杨明磊;晁江涛;王大伟;胡军华;吴华;龚达平;刘贯山;;遗传(第04期);第337-349页 *

Also Published As

Publication number Publication date
CN116376911A (en) 2023-07-04

Similar Documents

Publication Publication Date Title
CN107881172B (en) Stress inducible promoter, stress inducible promoter plant expression vector and method for inducing expression of target gene
CN103667339B (en) Application of rice-derived protein OsMKK4 and related biological material thereof to regulation and control of plant panicle types
CN113430212B (en) Apple rootstock salt stress resistance related gene MdLysMe3 and encoding protein and application thereof
CN106674338B (en) Application of stress resistance-related protein in regulation and control of plant stress resistance
CN112626069A (en) Soybean gma-miR4359b gene, expression vector thereof, preparation method and application thereof
CN116376911B (en) Plant drought, low temperature and osmotic stress induced promoter and application thereof
CN108486112B (en) Promoter with anther tissue specificity
CN108424912B (en) Promoter for low-phosphorus stress induced expression of corn and application thereof
CN108795942B (en) Rice exogenous stress induced expression promoter Psubs3 and application thereof
CN108165552B (en) Drought-inducible promoter PvHVA1-pro of plant and application thereof
CN100366743C (en) Plant-disease-resistance related protein and genes encoding same and use thereof
CN104152454A (en) Soybean derived drought induced type promoter GmMYB363P and application thereof
CN111718938B (en) High-temperature inducible promoter specifically expressed by plant green tissue and application thereof
CN116478998B (en) Rice phloem specific expression promoter POs04g0452500 and application thereof
CN113416732B (en) Dendrobium officinale salt inducible promoter proDoMYB75 and application thereof
CN102453719B (en) Plant bidirectional promoter BIGDB1
CN116004622B (en) Promoter of cotton APR gene, acquisition method and application thereof, fusion vector, preparation method and application
CN103788187A (en) Plant flowering related protein GmSOC1-like, coding gene and application thereof
CN103757025B (en) The promotor of an adverse circumstance abduction delivering and application thereof
CN115725603A (en) Anthurium stress-resistance related transcription factor and application thereof
CN114350675B (en) LuNAC gene for regulating and controlling synthesis of flax secondary wall and application thereof
CN111454987B (en) Application of GhNAC091 gene in improving plant photosynthesis efficiency and strong light tolerance
CN117187258A (en) Application of OsBEE1 gene in enhancing drought resistance of rice
CN116813738A (en) Application of GmNAC120 gene in negative regulation of soybean salt stress response
CN115927324A (en) Strong promoter CP17 specifically expressed in anther of plant in each period and application thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant