CN116327650A

CN116327650A - Peony compound soothing factor and preparation method and application thereof

Info

Publication number: CN116327650A
Application number: CN202310029300.1A
Authority: CN
Inventors: 韩婕珺; 龚天贵; 王斌; 陈贤
Original assignee: Guangzhou Jianeng Biological Technology Co ltd; Meichulai Hangzhou Cosmetics Co ltd
Current assignee: Guangzhou Jianeng Biological Technology Co ltd; Meichulai Hangzhou Cosmetics Co ltd
Priority date: 2023-01-09
Filing date: 2023-01-09
Publication date: 2023-06-27

Abstract

The invention relates to the technical field of plant extraction additives, and particularly discloses a peony compound relief factor and a preparation method and application thereof, wherein the preparation method of the peony compound relief factor comprises the following steps: extracting flos moutan by distillation; mixing the residues of tree peony branches and leaves, tree peony roots, licorice roots, pine needle, red plum leaves and tree peony flowers with water, adding an adsorption material, and circularly extracting; separating to obtain the adsorbed adsorption material and medicinal suspension; analyzing and filtering the adsorbed adsorption material to obtain a first filtrate; filtering the medicinal material suspension film, flocculating, centrifuging, concentrating, loading on a resin column to obtain a first permeate, and eluting with an eluent to obtain an eluent; mixing the first permeate and the peony hydrosol, fermenting, centrifuging, taking supernatant, and filtering by a membrane to obtain a second permeate; mixing the first filtrate, the eluent and the second permeate, concentrating, and sterilizing to obtain the peony compound relief factor. The invention has the advantage of improving the stability and the efficacy of the peony soothing factor.

Description

Peony compound soothing factor and preparation method and application thereof

Technical Field

The invention relates to the field of plant extraction additives, in particular to a peony compound relief factor, a preparation method and application thereof.

Background

The increasing proportion of skin allergy and barrier fragility has become one of the important concerns of personal care products because of the effects of modern living environment pollution, cosmetics, frequent cleaning, etc., while the natural source of soothing and repairing ingredients has become the core of the solution. Peony is the most frequently used drug in such demands. Peony has a long history of medicinal use, shennong Ben Cao Jing: treating carbuncle sore; drug Property theory (L): pain relieving; japanese Sunji (Japanese Subtu) grass: eliminating stagnation of blood. Modern researches show that peony has obvious effect on TNF-alpha, IL-1 beta, IL6, IL-17, COX-2, MAPK, NF- κB and other targets or signal paths.

The main components of the peony whole plant comprise root bark containing paeoniflorin (paeonol) and other saponins, paeonol (paeonol), polysaccharide, protein and other components.

However, peony has some defects in the application process, and the main active ingredients of the peony, such as paeonol, have the characteristics of low melting point, low volatility, poor water solubility and the like, and the main ingredient paeoniflorin is alkaline and easy to hydrolyze, so that the stability of the main active ingredients of the peony is poor in the preparation and use processes; the proteins and the polysaccharide are not easy to stay and absorb due to the large molecular weight of the proteins and the polysaccharide. Therefore, there is room for improvement.

In addition, the application process of the traditional Chinese medicine adopts a compound form generally, so that synergistic effect can be achieved, but interaction of substance bases among the medicinal materials in the compound is generally ignored in the extraction and separation process, and research on the phenomenon is likely to generate effects of improving efficiency and yield, enhancing stability, enhancing efficiency, releasing slow release and the like, so that the traditional Chinese medicine has guiding significance for reality.

Disclosure of Invention

In order to improve stability and efficacy of peony soothing factors, the application provides a peony compound soothing factor, a preparation method and application thereof.

In a first aspect, the present application provides a method for preparing a peony compound relief factor, which adopts the following technical scheme:

a preparation method of a peony compound soothing factor comprises the following steps:

step 1), distilling and extracting the peony, and filtering to obtain peony residues and peony hydrosol;

step 2), mixing the residues of the tree peony branches and leaves, the tree peony roots, the licorice roots, the pine needle and the tree peony flowers with 6-8 times of water, adding an adsorption material, and circularly extracting to obtain a mixture;

step 3), separating the mixture through a pipeline with electric conversion to obtain an adsorption material and a medicinal suspension after adsorption;

step 4), stirring and analyzing the adsorbed adsorbing material with an eluent with the volume of 3-4 times, forming ionic salt, and filtering by a membrane to obtain a first filtrate;

Step 5), filtering the medicinal material suspension through a membrane to obtain a second filtrate;

step 6), flocculating the second filtrate, centrifuging and taking supernatant;

step 7), concentrating the supernatant, loading the concentrated supernatant on a resin column to obtain a first permeate, and eluting the first permeate with an eluent to obtain an eluent;

step 8), mixing the first permeate and the peony hydrosol, and then inoculating a strain for fermentation to obtain a fermentation liquor;

step 9), centrifuging the fermentation liquor, taking supernatant, and performing membrane filtration to obtain a second permeate;

step 10), mixing the first filtrate in the step 4), the eluent in the step 7) and the second permeate in the step 9), concentrating, and sterilizing to obtain the peony compound relief factor.

By adopting the technical scheme, the efficacy synergy of multiple targets is realized by compounding the red plum and the licorice, inflammatory factors such as trpv1, hyaluronidase and the like are inhibited, itching caused by histamine is inhibited, skin barrier repair is promoted, and heavy metal stress resistance of cells is enhanced.

The main component glycyrrhizic acid of the licorice is easy to extract under alkaline condition, the pine-red plum alkaloid is easy to extract under acidic condition, and the paeonol belongs to weak acidity, small water solubility and easy volatility, based on which, three active substances with different properties are synchronously separated and protected in the extraction process by adopting an adsorption material, thereby realizing compatible extraction, improving the extraction rate and reducing the loss.

After the adsorption material is eluted by the eluent, paeonol/glycyrrhizic acid/pine red plum alkaloid is formed into ion salt to promote dissolution in the resolving process, so that three components with different dissolution characteristics are dissolved in the same system, and the ion salt is formed to have the functions of solubilization, stability and slow release.

Through the fermentation process, the macromolecular sugar and protein in the medicinal materials form oligosaccharide and oligopeptide with smaller molecular weight, so that the medicinal materials have better synergistic effect, better repairing and relieving effects, fully utilize resources and generate better skin care effect.

By adopting the process of transferring ionic salt and saponins and fermenting again, the situation that paeonol and other components in the compound block the growth of bifidobacteria during the direct fermentation of medicinal materials or extracting solution is avoided.

The flower has a large proportion of easily oxidized unstable components, stable water-soluble components can be obtained by adopting distillation and used as a solvent, so that the concentration of the process is reduced, the energy consumption is saved, and an anhydrous formula is formed.

The peony compound relief factor obtained by extracting the peony branches and leaves, the peony roots, the licorice roots, the pine needle and the peony flowers in a special proportion by compounding and matching with a special extraction process has higher stability, can better exert efficacy in shelf life, has higher extraction rate of the final product and has better economic value.

Preferably, the peony compound relief factor is prepared from the following components in percentage by mass:

component A:

peony flower: peony leaves= (8-10): 1, a step of;

component B:

root of peony: radix Glycyrrhizae Preparata: pine red plum leaf=9-10: 3-4:1, a step of;

the component A comprises the following components: component b=1: (1-3).

By adopting the technical scheme, the peony compound relief factor has better relief and restoration effects, better product stability and better product quality by specifically selecting the proportion of each component.

Preferably, in the step 2), the preparation method of the adsorption material includes the following steps:

step 01), acetonitrile, water, acrylic acid and target molecules are added into a container, stirred and dissolved, and then ferroferric oxide nano particles are added, and stirred uniformly to obtain a mixed solution;

step 02), adding N, N-methylene bisacrylamide and azodiisobutyronitrile into the mixed solution, stirring and performing ultrasonic dispersion to obtain a suspension;

step 03), reacting the suspension for 2-3 hours at 70-72 ℃, filtering, washing with acetic acid, balancing with methanol for 1-2 hours, and drying to obtain an adsorption material;

the target molecule can be glycyrrhizic acid or paeonol or pine-red plum alkaloid A, and the glycyrrhizic acid-adsorbing material, the paeonol-adsorbing material and the pine-red plum alkaloid A-adsorbing material are respectively prepared.

By adopting the technical scheme, the glycyrrhizic acid-adsorbing material, the paeonol-adsorbing material and the pine-red plum alkaloid A-adsorbing material are respectively prepared by adopting special processes, and three active substances with different properties are synchronously separated and protected in the extraction process by the glycyrrhizic acid-adsorbing material, the paeonol-adsorbing material and the pine-red plum alkaloid A-adsorbing material, so that compatible extraction is realized, the extraction rate is improved and the loss is reduced.

Preferably, in the step 01), the mass ratio of the acrylic acid, acetonitrile, water and the ferroferric oxide nano particles is 4:64:100:5, a step of; the molar ratio of the target molecule, acrylic acid, N-methylene bisacrylamide and azodiisobutyronitrile is 3:22:6:6.

by adopting the technical scheme, the prepared adsorption material has better effect of specifically adsorbing and protecting different active substances, so that the stability of the final product is better.

Preferably, in step 2) of the preparation method of the peony compound relief factor, the adsorption material is a compound of glycyrrhizic acid-adsorption material, paeonol-adsorption material and pine-red plum alkaloid A-adsorption material, and the mass ratio of glycyrrhizic acid-adsorption material, paeonol-adsorption material and pine-red plum alkaloid A-adsorption material is 1:5:1.5.

By adopting the technical scheme, the paeonol/glycyrrhizic acid/pine-red plum alkaloid is better formed into ionic salt in the analysis process by matching the glycyrrhizic acid-adsorbing material, the paeonol-adsorbing material and the pine-red plum alkaloid A-adsorbing material in a specific proportion, so that the dissolution is better promoted, and the dissolution effect of three components with different dissolution characteristics in the same system is better.

Preferably, in step 2) of the preparation method of the peony compound relief factor, the volume ratio of the input adsorption material to the input component B is 1:1-3, and the extraction time is 2-3h.

By adopting the technical scheme, the effect of protecting three components with different dissolution characteristics is better, the extraction effect is better, and the stability of the extract is better by specifically selecting the input amount of the adsorption material.

Preferably, in step 2) of the preparation method of the peony compound relief factor, during the cyclic extraction, the cyclic extraction is formed from bottom to top, and the medicinal material particles are controlled to be in an expanded bed state for the cyclic extraction.

Through adopting above-mentioned technical scheme, be the expanded bed state through control medicinal material granule, solvent and adsorption material carry out cyclic motion for medicinal material granule and adsorption material gravity difference produce the collision and make medicinal material and solvent produce relative motion, avoid the relatively stationary motion of conventional circulation, improve extraction efficiency and adsorption efficiency.

Preferably, in the step 4) and the step 7) of the preparation method of the peony compound relief factor, the eluent is the compounding of the peony hydrosol prepared in the step 1) and the 1,3 butanediol, and the mass ratio of the peony hydrosol to the 1,3 butanediol is 4:6.

by adopting the technical scheme, the peony hydrosol and the 1,3 butanediol are compounded to prepare the special eluent, so that paeonol/glycyrrhizic acid/pine red plum alkaloid can better form ionic salt in the analysis process, and the solubility of each component in a system can be better improved.

Preferably, in step 8) of the preparation method of the peony compound relief factor, the strain adopts bifidobacterium longum for anaerobic fermentation during fermentation.

By adopting the technical scheme, the fermentation is performed by inoculating bifidobacteria, the fermentation effect is better, the macromolecular sugar and protein in the medicinal material better form the oligosaccharide and oligopeptide with smaller molecular weight, and better skin care effect can be generated.

In a second aspect, the present application provides a peony compound relief factor, which adopts the following technical scheme:

a peony compound relief factor is prepared by the preparation method of the peony compound relief factor.

By adopting the technical scheme, the prepared peony compound relief factor has better relief and restoration effects, higher stability and better quality.

In a third aspect, the application provides an application of a peony compound relief factor, which adopts the following technical scheme:

the application of the peony compound soothing factor is added into personal care products to prepare the personal care products containing the peony compound soothing factor.

The application of the peony compound relief factor is added into food or health care products to prepare the food or health care products containing the peony compound relief factor.

The application of the peony compound relief factor is added into medicines to prepare medicines containing the peony compound relief factor.

By adopting the technical scheme, the product containing the peony compound relieving factor can play a good role in relieving and repairing, and the stability of the active ingredients is high, the effects of relieving and repairing can be continuously exerted within the shelf life of the product, and the quality is good.

In summary, the present application has the following beneficial effects:

1. because the peony compound relief factor obtained by extracting the peony branches and leaves, the peony roots, the licorice roots, the pine red plum leaves and the peony flowers in a compounding way by adopting a special proportion and a special extraction process is matched, the peony compound relief factor has higher stability, can better exert efficacy in shelf life, has higher extraction rate of the final product, and has better economic value.

2. In the application, the glycyrrhizic acid-adsorbing material, the paeonol-adsorbing material and the pine-red plum alkaloid A-adsorbing material are preferably prepared by adopting special processes, and three active substances with different properties are synchronously separated and protected in the extraction process by the glycyrrhizic acid-adsorbing material, the paeonol-adsorbing material and the pine-red plum alkaloid A-adsorbing material, so that compatible extraction is realized, the extraction rate is improved, and the loss is reduced.

3. In the application, the fermentation process is preferably adopted, so that the macromolecular sugar and protein in the medicinal material form the oligosaccharide and oligopeptide with smaller molecular weight, the oligosaccharide and oligopeptide have better synergistic effect, better repairing and relieving effects, and better skin care effect by fully utilizing resources.

Detailed Description

The present application is described in further detail below with reference to examples.

Example 1

The peony compound relief factor is prepared by extracting the following components in percentage by mass:

component a, comprising: peony flower: peony branches and leaves=10:1; component B, comprising: root of peony: radix Glycyrrhizae Preparata: pinus sylvestris leaf=9:3:1; component A: component b=1:3.

Namely 10kg of peony, 1kg of peony branches and leaves, 27kg of peony root, 9kg of licorice root, and 3kg of pine needle.

The preparation method of the peony compound relieving factor comprises the following steps:

step 1), smashing the peony into coarse powder with 5-10 meshes, adding 15 times of purified water for distillation and extraction, and centrifugally filtering to obtain peony residue and peony hydrosol with 10 times of peony mass.

Step 2), smashing tree peony branches and leaves, tree peony roots, swelling fruit licorice roots and pine red plum leaves into 5-10 mesh coarse powder, mixing with tree peony residues, adding water with the dry weight of 6 times of medicinal materials (tree peony branches and leaves, tree peony roots, swelling fruit licorice roots, pine red plum leaves and tree peony flowers), mixing and extracting at 50 ℃, adopting a circulating pump to form circulating extraction from bottom to top below an extracting tank, controlling medicinal material particles to be in an expanded bed state, and simultaneously adding an adsorbing material, wherein the adsorbing material is a compound of glycyrrhizic acid-adsorbing material, paeonol-adsorbing material and pine red plum alkaloid A-adsorbing material, and the mass ratio of the glycyrrhizic acid-adsorbing material, the paeonol-adsorbing material and the pine red plum alkaloid A-adsorbing material is 1:5:1.5, the total input volume of the adsorption material is consistent with the total input volume of the component B (peony root, licorice root, pine-red plum leaf), the expansion height is 1.5 times of the self height, the extraction time is 3 hours, and the mixture is obtained by cyclic extraction.

And 3) after the extraction is finished, separating the mixture through a pipeline with electric conversion, and separating the adsorbed adsorbing material and medicinal suspension.

Step 4), mixing the adsorbent material with an eluent with the volume of 4 times that of the adsorbent material, wherein the eluent is the compounding of the peony hydrosol and the 1, 3-butanediol, and the mass ratio of the peony hydrosol to the 1, 3-butanediol is 4: and 6, stirring and resolving for 6 hours at 40 ℃ to form ionic salt, and performing membrane filtration through a 0.2-micrometer membrane to obtain a first filtrate, and recovering the adsorption material.

And 5) performing membrane filtration on the medicinal material suspension by using a 0.45-micrometer membrane to obtain a second filtrate.

And 6) adding 0.5 per mill of chitosan into the second filtrate for flocculation, stirring for 0.5h at normal temperature, refrigerating for 24h at 8 ℃, centrifuging for 15min at 4000 turns, and taking supernatant.

Step 7), concentrating the supernatant until the mass is consistent with the total input mass of medicinal materials, then loading the supernatant onto a D101 macroporous resin column at a loading speed of 3BV/h, wherein the dosage of resin is 1 time of the input mass of peony roots, collecting a loading permeate to obtain a first permeate, then cleaning the macroporous resin column with pure water with a volume of 2 times of the column, eluting the macroporous resin column with an eluent with a volume of 5 times of the column, wherein the eluting speed is 2BV/h, the eluent is the compounding of peony hydrosol and 1, 3-butanediol, and the mass ratio of the peony hydrosol to the 1, 3-butanediol is 4:6, obtaining eluent.

Step 8), mixing the first permeate and the rest peony hydrolat to obtain a composite liquid, fermenting the composite liquid, selecting bifidobacterium longum Bifidobacterium longum as a strain during fermentation, reactivating the bifidobacterium, transferring to an MRS culture medium, performing amplification culture after standing culture at 37 ℃, controlling the pH at 37 ℃ for 10 hours, adding the composite liquid at a speed of 1% tank volume/h, controlling the pH at 5, continuing anaerobic culture for 8 hours, and then not controlling the pH at 37 ℃ again, and performing anaerobic culture for 6 hours to obtain a fermentation liquor;

step 9), centrifuging 4000 turns of the fermentation broth for 15min, taking supernatant, adding 0.75% of 200-mesh active carbon, stirring for 1h at normal temperature, performing membrane filtration by using a 0.2 mu m membrane, and performing ultrafiltration by using a 10 ten thousand Dalton membrane at a pressure of 2.5bar to obtain a second permeate;

step 10), mixing the first filtrate in the step 4), the eluent in the step 7) and the second permeate in the step 9), concentrating by using a 150 dalton nanofiltration membrane until the mass is 2 times of the total input mass of the medicinal materials, preserving heat for 0.5h at 95 ℃, sterilizing, cooling to below 50 ℃, and passing through a 0.1 mu m membrane to obtain the peony compound relief factor.

The preparation method of the adsorption material used in the step 2) comprises the following steps:

And 01) adding acetonitrile, water, acrylic acid and target molecules into a container, stirring and dissolving, then adding ferroferric oxide nano particles, and stirring uniformly to obtain a mixed solution.

Step 02), adding N, N-methylene bisacrylamide and azodiisobutyronitrile into the mixed solution, stirring and performing ultrasonic dispersion to obtain a suspension.

Step 03), reacting the suspension for 2 hours at 70 ℃, filtering, washing with acetic acid, balancing with methanol for 1 hour, drying and crushing to obtain the adsorption material.

Wherein, the input mass ratio of the acrylic acid, acetonitrile, water and ferroferric oxide nano particles is 4:64:100:5.

wherein, the input mole ratio of the target molecule, the acrylic acid, the N, N-methylene bisacrylamide and the azodiisobutyronitrile is 3:22:6:6.

the target molecule can be glycyrrhizic acid or paeonol or pine-red plum alkaloid A, and the glycyrrhizic acid-adsorbing material, paeonol-adsorbing material and pine-red plum alkaloid A-adsorbing material are respectively prepared according to the selection of the target molecule. The glycyrrhizic acid-adsorbing material, paeonol-adsorbing material and pine and red plum alkaloid A-adsorbing material are prepared independently.

Example 2

Component a, comprising: peony flower: peony branches and leaves=8:1; component B, comprising: root of peony: radix Glycyrrhizae Preparata: pinus sylvestris leaf=9:3:1; component A: component b=1:1.

Namely, 8kg of peony, 1kg of peony branches and leaves, 9kg of peony root, 3kg of licorice root, and 1kg of pine needle and red plum leaves.

Step 2), smashing tree peony branches and leaves, tree peony roots, swelling fruit licorice roots and pine red plum leaves into 5-10 mesh coarse powder, mixing with tree peony residues, adding water with the dry weight of 8 times of medicinal materials (tree peony branches and leaves, tree peony roots, swelling fruit licorice roots, pine red plum leaves and tree peony flowers), mixing and extracting at the extraction temperature of 40 ℃, adopting a circulating pump to form circulating extraction from below an extraction tank to the top, controlling medicinal material particles to be in an expanded bed state, and simultaneously adding an adsorbing material, wherein the adsorbing material is a compound of glycyrrhizic acid-adsorbing material, paeonol-adsorbing material and pine red plum alkaloid A-adsorbing material, and the mass ratio of the glycyrrhizic acid-adsorbing material, paeonol-adsorbing material and pine red plum alkaloid A-adsorbing material is 1:5:1.5, the total input volume of the adsorption material is 1/3 of the total input volume of the component B (peony root, licorice root, pine-red plum leaf), the expansion height is 2.5 times of the self height, the extraction time is 2.5h, and the mixture is obtained by circulating extraction.

Step 4), mixing the 3 times volume of eluent with the adsorbed adsorbing material, wherein the eluent is the compounding of the peony hydrosol and the 1, 3-butanediol, and the mass ratio of the peony hydrosol to the 1, 3-butanediol is 4: and 6, stirring and resolving for 4 hours at 50 ℃ to form ionic salt, and performing membrane filtration through a 0.2-micrometer membrane to obtain a first filtrate, and recovering the adsorption material.

And 6) adding 0.3 per mill of chitosan into the second filtrate for flocculation, stirring for 0.5h at normal temperature, refrigerating for 24h at 8 ℃, centrifuging for 15min at 4000 turns, and taking supernatant.

Step 7), concentrating the supernatant until the mass is consistent with the total input mass of medicinal materials, then loading the supernatant onto a D101 macroporous resin column at a loading speed of 3BV/h, wherein the dosage of resin is 2 times of the input mass of peony roots, collecting a loading permeate to obtain a first permeate, then cleaning the macroporous resin column with pure water with a volume of 2 times of the column, eluting the macroporous resin column with an eluent with a volume of 5 times of the column, wherein the eluting speed is 2BV/h, the eluent is the compounding of peony hydrosol and 1, 3-butanediol, and the mass ratio of the peony hydrosol to the 1, 3-butanediol is 4:6, obtaining eluent.

step 9), centrifuging 4000 turns of the fermentation broth for 15min, taking supernatant, adding 0.5% 300-mesh active carbon, stirring for 1h at normal temperature, performing membrane filtration by using a 0.2 mu m membrane, and performing ultrafiltration by using a 10 ten thousand Dalton membrane under the pressure of 2bar to obtain a second permeate;

Step 03), reacting the suspension for 2.5 hours at 71 ℃, filtering, washing with acetic acid, balancing with methanol for 1.5 hours, drying and crushing to obtain the adsorption material.

Example 3

Component a, comprising: peony flower: peony branches and leaves=9:1; component B, comprising: root of peony: radix Glycyrrhizae Preparata: pinus sylvestris leaf=9:3:1; component A: component b=1:2.

Namely 9kg of peony, 1kg of peony branches and leaves, 18kg of peony root, 6kg of licorice root, and 2kg of pine needle and red plum leaves.

Step 2), smashing tree peony branches and leaves, tree peony roots, swelling fruit licorice roots and pine red plum leaves into 5-10 mesh coarse powder, mixing with tree peony residues, adding water with the dry weight of 7 times of medicinal materials (tree peony branches and leaves, tree peony roots, swelling fruit licorice roots, pine red plum leaves and tree peony flowers), mixing and extracting at the temperature of 45 ℃, adopting a circulating pump to form circulating extraction from below an extraction tank to top, controlling medicinal material particles to be in an expanded bed state, and simultaneously adding an adsorbing material, wherein the adsorbing material is a compound of glycyrrhizic acid-adsorbing material, paeonol-adsorbing material and pine red plum alkaloid A-adsorbing material, and the mass ratio of the glycyrrhizic acid-adsorbing material, the paeonol-adsorbing material and the pine red plum alkaloid A-adsorbing material is 1:5:1.5, the total input volume of the adsorption material is 1/2 of the total input volume of the component B (peony root, licorice root, pine-red plum leaf), the expansion height is 2 times of the self height, the extraction time is 2 hours, and the mixture is obtained by cyclic extraction.

Step 4), mixing the 3.5 times volume of eluent with the adsorbed adsorbing material, wherein the eluent is the compounding of the peony hydrosol and the 1, 3-butanediol, and the mass ratio of the peony hydrosol to the 1, 3-butanediol is 4: and 6, stirring and resolving for 5 hours at 45 ℃ to form ionic salt, and performing membrane filtration through a 0.2-micrometer membrane to obtain a first filtrate, and recovering the adsorption material.

And 6) adding 0.4 per mill of chitosan into the second filtrate for flocculation, stirring for 0.5h at normal temperature, refrigerating for 24h at 8 ℃, centrifuging for 15min at 4000 turns, and taking supernatant.

Step 8), mixing the first penetrating fluid and the rest peony hydrolat to obtain a compound fluid, then fermenting the compound fluid, during fermentation, selecting bifidobacterium longum Bifidobacterium longum, reactivating the bifidobacterium, transferring to an MRS culture medium, performing amplification culture after standing culture at 37 ℃, controlling the pH at 37 ℃ for 10 hours, adding the compound fluid, controlling the pH at 5 at a speed of 1% tank volume/hour, continuing anaerobic culture for 8 hours, and then not controlling the pH at 37 ℃ again, and performing anaerobic culture for 6 hours to obtain a fermentation fluid.

Step 9), centrifuging the fermentation liquor 4000 for 15min, taking supernatant, adding 1% of 200-mesh active carbon, stirring for 1h at normal temperature, performing membrane filtration by using a 0.45 mu m membrane, and performing ultrafiltration by using a 10 ten thousand Dalton membrane under the pressure of 1.5bar to obtain a second permeate.

Step 03), reacting the suspension for 3 hours at 72 ℃, filtering, washing with acetic acid, balancing with methanol for 2 hours, drying and crushing to obtain the adsorption material.

Comparative example 1

The peony complex soothing factor differs from example 1 only in that:

The peony compound relieving factor is prepared by extracting the following components in percentage by mass:

component a, comprising: peony flower: peony branches and leaves=7:1; component B, comprising: root of peony: radix Glycyrrhizae Preparata: pinus sylvestris leaf=10:4:1; component A: component b=1:0.5.

Namely, 14kg of peony, 2kg of peony branches and leaves, 20kg of peony root, 8kg of licorice root, and 1kg of pine needle.

Comparative example 2

The peony complex soothing factor differs from example 1 only in that:

component a, comprising: peony flower: peony branches and leaves=11:1; component B, comprising: root of peony: radix Glycyrrhizae Preparata: pinus sylvestris leaf=8:2:1; component A: component b=1:4.

Namely 11kg of peony, 1kg of peony branches and leaves, 32kg of peony root, 8kg of licorice root, and 4kg of pine needle and red plum leaves.

Comparative example 3

The peony complex soothing factor differs from example 1 only in that:

And 4) mixing the adsorbent material with the eluent with the volume which is 4 times that of the adsorbent material, stirring and resolving for 6 hours at the temperature of 40 ℃ to form ionic salt, and performing membrane filtration through a 0.2 micrometer membrane to obtain a first filtrate, and recovering the adsorbent material.

And 7) concentrating the supernatant until the mass is consistent with the total input mass of the medicinal materials, loading the supernatant onto a D101 macroporous resin column at a loading speed of 3BV/h, wherein the dosage of the resin is 1 time of the input mass of the peony root, collecting the loading permeate to obtain a first permeate, and eluting the macroporous resin column with an eluent with a volume of 5 times of the column at an eluting speed of 2BV/h to obtain an eluent.

And 8) mixing the first penetrating fluid and the rest peony flower hydrosol to obtain a composite fluid.

Step 9), centrifuging 4000 turns of the composite liquid for 15min, taking supernatant, adding 0.75% of 200-mesh active carbon, stirring for 1h at normal temperature, performing membrane filtration by using a 0.2 mu m membrane, and performing ultrafiltration by using a 10 ten thousand Dalton membrane at a pressure of 2.5bar to obtain a second permeate;

Comparative example 4

The peony complex soothing factor differs from example 1 only in that:

step 01), pulverizing medicinal materials (peony, peony branches and leaves, peony root, licorice root, pine-red plum leaves) into 5-10 mesh coarse powder, adding water 10 times of the dry weight of the medicinal materials, stirring and extracting at 80-90 ℃ for 3 hours, and then performing membrane filtration with a 0.45-micrometer membrane to obtain filtrate.

Step 02), adding 0.5 per mill of chitosan into the filtrate for flocculation, stirring at normal temperature for 0.5h, refrigerating at 8 ℃ for 24h, centrifuging at 4000 r for 15min, and obtaining a first supernatant;

step 03), concentrating the first supernatant until the quality is consistent with the total input amount of the medicinal materials, then loading the concentrated first supernatant on a D101 macroporous resin column, wherein the loading speed is 3BV/h, the resin dosage is 2 times of the total input amount of the medicinal materials, collecting the permeate, eluting the permeate by using 1, 3-butanediol with the concentration of 60% as an eluent, wherein the eluent dosage is 5 times of the column volume, and the eluting speed is 2BV/h, thereby obtaining the eluent.

Step 04), dragging overnight, centrifuging for 15min at 4000 rpm to obtain a second supernatant, adding 0.75% of 200-mesh active carbon, stirring at normal temperature for 1h, performing membrane filtration by using a 0.2 mu m membrane, and performing ultrafiltration by using a 10 ten thousand Dalton membrane under a pressure of 2.5bar to obtain a permeate.

Step 05), mixing the eluent in the step 03) and the permeate in the step 04, concentrating the mixture by using a 150 dalton nanofiltration membrane until the total input amount of the medicinal materials is 1/2, preserving heat for 0.5h at 90-95 ℃, cooling the mixture to below 50 ℃, and passing through a 0.1 mu m membrane to obtain the peony compound relief factor.

Comparative example 5

The peony complex soothing factor differs from example 1 only in that:

step 001), pulverizing the medicinal materials (peony, peony branches and leaves, peony root, licorice root, pine needle and plum leaves) into 5-10 mesh coarse powder, adding water 10 times of the dry weight of the medicinal materials, stirring and extracting at 80 ℃ for 3 hours. The filtrate was membrane filtered with a 0.45 micron membrane.

Step 002), adding 0.5 per mill chitosan into the filtrate for flocculation, stirring for 0.5h at normal temperature, refrigerating for 24h at 8 ℃, centrifuging for 15min at 4000 turns, and obtaining supernatant.

Step 003), concentrating the supernatant until the mass is consistent with the dry weight of the medicinal materials, then loading the supernatant onto a D101 macroporous resin column at a loading speed of 3BV/h, wherein the resin dosage is 2 times of the total mass of the medicinal materials, and collecting a permeate.

Step 004), eluting with 60% 1, 3-butanediol as eluent, wherein the amount of the eluent is 5 times of the column volume, the eluting speed is 2BV/h, and collecting the eluent.

Step 005), fermenting the permeate, wherein during fermentation, the strain selects bifidobacterium longum Bifidobacterium longum, reviving the bifidobacterium and transferring to an MRS culture medium, performing amplification culture after standing culture at 37 ℃, performing constant temperature of 37 ℃, controlling pH to 6.5, performing anaerobic culture for 10 hours, adding a composite liquid, adding the composite liquid at a speed of 1% tank volume/h, controlling pH to 5, continuing anaerobic culture for 8 hours, and performing anaerobic culture for 6 hours without controlling pH, thereby obtaining the fermentation broth.

Step 006), centrifuging the fermentation liquor 4000 for 15min, adding 0.75% of 200-mesh active carbon, stirring at normal temperature for 1h, performing membrane filtration by using a 0.2 mu m membrane, and then performing ultrafiltration by using a 10 ten thousand Dalton membrane at a pressure of 2.5bar to obtain a permeate.

Step 007), mixing the eluent in step 004) and the permeate in step 006), concentrating with a 150 dalton nanofiltration membrane to 1/2 of the total mass of the medicinal materials, preserving heat for 0.5h at 95 ℃, cooling to below 50 ℃, and passing through a 0.1 mu m membrane to obtain the peony compound relief factor.

Experiment 1

Stability test (d): the sample to be measured has the longest time of no serious color change, no precipitation visible to naked eyes and less than or equal to 10 percent of total component content under the conditions of 4 ℃ and 48 ℃ and normal-temperature illumination.

Experiment 2

Component extraction rate and yield test: component group i=paeonol+glycyrrhizic acid+pine-red plum alkaloid+paeoniflorin; component group ii = total sugar + total protein; total component = component group i + component group ii.

Paeonol detection method refers to P172 of 2015 edition of Chinese pharmacopoeia; the paeoniflorin detection method refers to P105 of 2015 edition of Chinese pharmacopoeia; the glycyrrhizic acid detection method refers to P87 of 2015 edition of Chinese pharmacopoeia; the method for testing the total alkaloids comprises the following steps: self-building, ultraviolet spectrophotometry. The standard substance is pine and red plum alkaloid A (C) ₁₃ N ₂ O ₂ H ₁₆ )。

The standard solution or the sample dilution was placed in dry triangular flasks with stopper, each with water to 5ml, and 5ml of ph=7.6 buffer was added, respectively. Sequentially adding precisely 1.0ml of 0.025% bromothymol blue standard solution, 10.0ml of chloroform, shaking for 1min, pouring into a 50ml separating funnel, standing for 1 hr, and separating chloroform layer. The absorbance of each group was measured at a wavelength of 414 nm. And (3) making a standard curve according to the concentration and absorbance of the standard solution, and obtaining a regression equation of the standard curve: y=ax+b [ wherein x is the content (mg) of rubus pinnatifida alkaloid a, and Y is absorbance ].

Total sugar test method: phenol-sulfuric acid process (ultraviolet spectrophotometry); total protein testing method: kjeldahl nitrogen determination method; component extraction rate = component extraction mass ≡ theoretical mass of components in the drug material 100%; theoretical mass of components in the medicinal materials: pulverizing the medicinal materials into 80 mesh powder, adding 30 times of 50% ethanol solution, ultrasonic extracting for 30min, and filtering; repeating the extraction for 3 times, mixing the 4 times of extracting solutions, and testing the content of the components; yield = component extraction mass ≡ drug mass 100%.

The test data of experiments 1 and 2 are shown in Table 1.

TABLE 1

	Component group I extraction yield%	Yield of component group II%	Stability (d)
				Example 1	81.2	11.3	≥60
Example 2	77.6	10.9	≥60
				Example 3	79.4	11.7	≥60
Comparative example 1	62.4	10.1	15
				Comparative example 2	59.1	9.9	11
Comparative example 3	80.5	4.3	45
				Comparative example 4	51.3	3.5	7
Comparative example 5	52.2	5.4	10

According to the comparison of the extraction rate data of component I in Table 1, the extraction rate of the invention is far higher than that of the traditional method (comparative example 4), meanwhile, the ratio of the medicinal materials (comparative examples 1 and 2) affects the extraction effect, the reasons are related to the ratio of the components, under the specific ratio conditions of the examples, in the process of resolving to obtain the first filtrate, glycyrrhizic acid, paeonol and pine-red plum alkaloid form ion pairs, the dissolution capacity and stability are improved, and the phenomenon is also verified from the stability results.

As can be seen from the comparison of the yield data of component II in Table 1, the yield of the present invention is far higher than that of the conventional method (comparative example 4), and comparative example 3 has not been subjected to fermentation, and thus contains sugar and protein which are relatively large in molecular weight and are therefore incompatible in a butanediol-water system and thus greatly reduced in content, and the result of comparative example 5 shows the necessity of fermentation after separation into component I from the extract, and the presence of component I inhibits the activity of fermentation tubes.

From the stability data in Table 1, it can be seen that the stability of the product obtained according to the invention is far higher than that obtained by the conventional method, and also better than that obtained by the extraction only of the non-fermented one (comparative example 3), the smaller the molecular weight, the more stable.

Experiment 3

Histamine inhibition experiments: KM mice were randomly divided into 3 groups of 10 mice each, each half of which was male and female. The grouping situation is as follows: (1) model control (water), (2) positive control (compound dexamethasone acetate cream), and (3) sample. Dosage of: positive group 0.1 g/min, model group and sample group 0.2 ml/min.

Administration: after the adaptive feeding, the hairs at the back of the neck of the mouse are sheared off by scissors one day before the experiment, the hairs are unhaired by using unhairing paste to expose the skin, the area of the unhairing area is about 2cm x 2cm, and the mice with intact skin are taken for smearing and administration. The administration modes are all stay type administration, each administration is carried out 1 time a day, and the administration is carried out continuously for 2 days.

Itching: after the last administration for 1 hour, each group of mice was subcutaneously injected with 0.2ml (10 ml/kg) of 0.05% histamine phosphate physiological saline, and the front paws of the mice were observed to scratch the head, the rear paws scratch the trunk, and the mouth bites all over the body as an index of pruritus reaction.

Experimental records: recording the pruritus latency time of each mouse, the number of times of pruritus and the total duration of pruritus of each group of mice within 30min, calculating the pruritus inhibition rate, judging the itching relieving effect intensity of each test group according to the pruritus latency time, the inhibition rate and the pruritus duration, and detailing the experimental results in Table 2, wherein the calculation formula of the pruritus inhibition rate is as follows:

TABLE 2 Histamine inhibition assay results

Note that: * P <0.05 compared to model group, P <0.01 compared to model group, P <0.001.

From the data in Table 3, it is clear that the longer the latency, the less the scratch test, and the shorter the duration are all better indicators of performance. Examples 1 and 3 show a remarkable relieving and itching effect compared with the positive control on the 2 indexes of scratching inhibition rate and itching duration, and the latency index is remarkably superior to the positive control, and besides the beneficial effect of a specific proportion of the composition, the effects of the active compound ions formed by the invention can be related to a certain slow release effect. The effect of the examples is better than that of comparative examples 1 and 2, and the specific ratio is an important factor for the effect generation, and the effect is remarkably superior to that of comparative example 4, and the effect of the overall process of the invention is remarkably improved.

Experiment 4

TRPV1 inhibition assay: according to 1.8X10 ⁵ The keratinocytes were seeded at a seed/well seeding density into 6-well plates and incubated overnight in an incubator (37 ℃, 5% co 2). When the cell plating rate in the 6-hole plate reaches 40% -50%, group administration (simultaneously stimulated by capsaicin 15 mu M) is carried out, the administration amount of each hole is 2mL, and 3 compound holes are arranged in each group. After completion of the administration, the 6-well plate was placed in an incubator (37 ℃, 5% co 2) and incubated for 24 hours. Fixing with 4% paraformaldehyde for 30min, immunofluorescence detection of capsaicin receptor (TRPV 1) is performed, and the images are taken and analyzed under microscope. GraphPad Prism was used to map and the results were expressed as mean±sd. Comparisons between groups were performed using t-test statistical analysis (double tailed). Compared with the model group, P <0.05 is considered to have a significant difference, expressed as:, P<0.01 is considered to have a very significant difference, expressed as x. Compared with blank group, P<0.01 is considered to have a very significant difference, expressed as # and the experimental results are detailed in table 3.

TABLE 3 summary of TRPV1 protein immunofluorescence analysis results

Remarks: integrated Optical Density (IOD)/cell number, the value of which reflects the relative content of TRPV1 protein. When the statistical analysis is performed by the t-test method, compared with the BC group, the significance is expressed as # and the P-value < 0.05 is expressed as # and the P-value < 0.01 is expressed as #; compared with NC groups, significance is expressed as x, P-value < 0.05 is expressed as x, and P-value < 0.01 is expressed as x.

Experiment 5

Hyaluronidase inhibition assay: taking 50 mu L of sample solution and 50 mu LHAase solution by adopting an HAase in-vitro inhibition test method, and carrying out heat preservation culture for 20min at 37 ℃; adding 50 mu L of calcium chloride solution, and continuing to perform heat preservation culture at 37 ℃ for 20min; adding 250 mu L of sodium hyaluronate solution, and preserving heat at 37 ℃ for 40min; adding 50 mu LNaOH solution and 100 mu L sodium borate solution, heating in boiling water bath for 3min, and immediately cooling to room temperature with ice water; 1.5mL of PDMAB solution was added thereto, incubated at 37℃for 20min, and cooled to room temperature. OD values were measured at 585nm, the experimental results are shown in Table 4, and the Haase inhibition rate was calculated as follows:

TABLE 4 results summary of HA enzyme test results

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Compared to BC group, significance is expressed as x, P-value < 0.05 is expressed as x, and P-value < 0.01 is expressed as x.

The lower the relative content of TRPV1 protein, the better the soothing effect and the higher the inhibition rate of hyaluronidase. As can be seen from tables 2, 3, the effects of examples 1, 3 are significantly better than those of comparative examples 1, 2, 4, illustrating that the specific ratios and process schemes of the present invention are the basis for achieving advantageous effects.

Experiment 6

Heavy metal stress resistance experiment: the sample was evaluated by MTT experiments using human immortalized keratinocytes (accession number CC-Y1177) for the ability to significantly increase cell viability in the presence of heavy metal Hg, model control aminomercuric chloride 34 μm, and the experimental results are detailed in table 5.

TABLE 5 summary of heavy metal treated cell survival results

Statistical analysis was performed using the t-test method, with significance expressed as P-value < 0.05, P-value < 0.001, P-value < 0.0001, ns, no significant difference compared to Hg.

Mercury can cause cell damage, causing a decrease in cell viability. After the examples 1 and 3 are added, the survival rate of the cells can be obviously improved, namely, the sample provided by the invention can improve the stress resistance of the cells, enhance the recovery capacity of the cells and has a repairing effect. As can be seen from the comparison of table 4, the examples are superior to comparative examples 1, 2, illustrating that the specific ratio has a significant effect on the results; the data of comparative examples 3 and 5 show that the cell stress-resistant effect is not substantially achieved by fermentation or by fermentation in the conventional process, which means that the fermentation produces the material basis of the effect, and the conventional fermentation process has poor effect because some components are not extracted and separated to inhibit the fermentation process.

Experiment 7

Skin mechanical barrier repair experiments: male nude mice were randomly grouped into 4 groups. The dosage of each group except the blank control group and the model control group is 0.2 ml/each group, and the blank control group, the model control group, the positive control group (Kang Fumei glycerol stock) and the test group (2% concentration) are respectively equal. After the model is successfully molded, the model control group is not treated, and other groups are sequentially dosed. The preparation method comprises the steps of 1 time of daily administration, 2 days of continuous administration, sampling skin at the back of each group of conventional operation at 48 hours, fixing skin tissues by 4% paraformaldehyde, trimming, dehydrating, embedding, slicing, dyeing, sealing, and finally microscopic examination of qualified samples, and observing pathological changes of tissue slices under different multiples by using a microscope. And photographing to observe the state of the skin wound surface. The test results are shown in detail in FIGS. 1-16.

Fig. 1 is a microscopic view of a blank set of skin tissues.

Fig. 2 is a microscopic image of the dermis layer of the blank group.

Fig. 3 is a microscopic image of skin tissue after 48h of the model group.

Fig. 4 is a microscopic image of the dermis layer after 48h of the model group.

Fig. 5 is a microscopic image of skin tissue 48h after the positive control group.

Fig. 6 is a microscopic image of the dermis layer 48h after the positive control group.

FIG. 7 is a microscopic image of skin tissue after 48h of the peony complex relief factor of comparative example 1.

FIG. 8 is a microscopic image of dermis layer after 48h using peony complex relief factor of comparative example 1.

FIG. 9 is a microscopic image of skin tissue after 48h using the peony complex relief factor of example 1.

Fig. 10 is a microscopic image of dermis layer after 48h using the peony complex relief factor of example 1.

FIG. 11 is a microscopic image of skin tissue after 48h of the peony complex relief factor of comparative example 5.

FIG. 12 is a microscopic image of the dermis layer after 48h using the peony complex relief factor of comparative example 5.

FIG. 13 is a microscopic image of skin tissue after 48h of the peony complex relief factor of comparative example 3.

FIG. 14 is a microscopic image of the dermis layer after 48h using the peony complex relief factor of comparative example 3.

FIG. 15 is a microscopic image of skin tissue after 48h using the peony complex relief factor of example 3.

FIG. 16 is a microscopic image of the dermis layer after 48h using the peony complex relief factor of example 3.

As can be seen from fig. 1-2, the skin tissue is seen to have intact epidermis and loose stratum corneum discharge; the dermis layer is orderly arranged by connective tissue, and the dermis layer is infiltrated by scattered lymphocyte; subcutaneous tissue is located below dermis and consists of loose connective tissue and adipose tissue, and the muscular fibers of the muscular layer are densely distributed, so that hair follicles, sweat glands and the like of the skin appendages are distributed in a scattered manner, and no obvious abnormality is seen.

As can be seen from fig. 3-4, skin tissue can be seen as necrotic epidermis, partial absence, cellulose-like necrosis of collagen fibers in the superficial dermis, partial disorder of collagen fiber arrangement, swelling and fracture, damage deep to the skin, necrosis of hair follicles, sweat glands, etc. which occur to different extents; the inflammatory response is severe and massive neutrophil infiltration and mononuclear cell infiltration are seen.

According to FIGS. 5-6, it can be seen that the skin tissue has a dry necrotic tissue residue, forms crust (the repaired epidermis is intact and uneven in thickness, the epidermis is less-arranged fibroblasts and collagen fibers, the inflammatory reaction is reduced, small amounts of new capillaries are seen in the dermis and subcutaneous tissue, neutrophils and lymphocytes are distributed in a scattered manner, the number of hair follicles is reduced, the subcutaneous connective tissue is less-arranged, and inflammatory cells are infiltrated in a large amount.

As can be seen from fig. 7-8, skin tissue can see epidermis necrosis, thinning of the spinous layer, partial epidermis deficiency, cellulose-like necrosis of the collagen fibers of the dermis shallow layer, partial collagen fiber swelling, disorder of arrangement, deep subcutaneous damage, necrosis of the skin appendages, hair follicles, sweat glands and the like with different occurrence degrees, and the number is reduced; the inflammatory reaction is serious, new capillary vessels appear, and a large amount of neutrophil granulocyte, monocyte and lymphocyte infiltrates are visible.

As can be seen from fig. 9-10, the skin tissue can see dry necrotic tissue residue, form crusts, the repaired epidermis is intact, the thickness is uneven, and even the stratum corneum is slightly repaired; the fibroblasts and collagen fibers are arranged in order under the epidermis; the inflammatory reaction is reduced, a small amount of new capillary vessels are seen in dermis and subcutaneous tissues, and neutrophils and lymphocytes are scattered.

As can be seen from fig. 11-12, skin tissue can see epidermis necrosis, thinning of the acantha layer, partial epidermis deficiency, cellulose-like necrosis of collagen fibers in the shallow dermis, swelling of collagen fibers in the shallow dermis, disorder of discharge, necrosis of the skin appendages hair follicles, sweat glands and the like in the dermis layer with different degrees of occurrence, and reduction in number; the inflammatory reaction is moderate, new capillary vessels appear, and infiltration of a large amount of neutrophils, monocytes and lymphocytes can be seen.

As can be seen from fig. 13-14, skin tissue can see epidermis necrosis, thinning of the acantha layer, partial epidermis deficiency, cellulose-like necrosis of collagen fibers in the shallow dermis, swelling of collagen fibers in the shallow dermis, disorder of discharge, necrosis of the skin appendages hair follicles, sweat glands and the like in the dermis layer with different occurrence degrees, and the number is reduced; the inflammatory reaction is moderate, new capillary vessels appear, and infiltration of a large amount of neutrophils, monocytes and lymphocytes can be seen.

As can be seen from fig. 15-16, the skin tissue can see dry necrotic tissue residue, form crusts, the repaired epidermis is intact, and the thickness is uneven; the fibroblast and collagen fiber with sparse arrangement are arranged under the epidermis; the inflammatory reaction is moderate, a small amount of new capillaries are seen in dermis and subcutaneous tissues, and neutrophils and lymphocytes are scattered and distributed; the number of multiple hair follicles is reduced; subcutaneous connective tissue is sparsely arranged with massive inflammatory cell infiltration.

In conclusion, as can be seen from comparison of 48h pathological sections, the effects of the examples 1 and 3 are equivalent to those of a positive control, the damage condition of skin tissues in a molding area is obviously improved, the epidermis is complete after 48h, the inflammation is obviously reduced, and the sample provided by the invention has an obvious effect of relieving and repairing. Whereas the 48h pathological sections of comparative examples 1, 3 and 5 all had damaged epidermis and severe inflammation existed, indicating that the specific formulation and process conditions of the present invention are the basis of the effect.

Application examples 1-2

The composition and the dosage (unit Kg) of the peony skin care emulsion are shown in Table 6.

TABLE 6

The preparation method of the peony skin care cream comprises the following steps:

step 1), adding deionized water after the premixing 1 of the phase A is prepared, heating to 85 ℃ in a stirring state, and stirring until the solution is completely dissolved.

And 2) adding the premix 2 of the phase B into an oil pot while heating the phase A, heating and stirring to 90 ℃ until the mixture is completely dissolved, adding sodium (ester) copolymer and lecithin, stirring and dispersing uniformly, adding into a main pot, homogenizing at medium speed for 5min, adding polydimethylsiloxane and polydimethylsiloxane alcohol, continuing homogenizing for 5min, and starting cooling.

And 3) cooling to 45 ℃, adding the C-phase raw material, stirring until the C-phase raw material is uniformly dispersed, and discharging.

The peony compound relief factor is the peony compound relief factor prepared in example 1, and in other application examples, the peony compound relief factor in examples 2-3 or each comparative example may also be used.

Application examples 3 to 4

The composition and the dosage (unit Kg) of the peony skin lotion are shown in Table 7.

TABLE 7

The preparation method of the peony skin lotion comprises the following steps:

step 1), adding the premix 1 of the phase A into deionized water after mixing, heating to 85 ℃ in a stirring state, stirring until the mixture is completely dissolved, adding the chlorobenzeneglycolether, and cooling after the dissolution.

And 2) cooling to 45 ℃, adding the phase B raw material, stirring until the mixture is uniformly dispersed, and discharging to obtain the peony skin care lotion.

Application examples 5 to 6

The composition and the dosage (unit Kg) of the peony essence are shown in Table 8.

TABLE 8

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The preparation method of the peony essence comprises the following steps:

step 1), adding acrylic acid (ester)/C10-30 alkanol acrylate crosslinked polymer into deionized water, heating after stirring for 15min, adding premix 1, heating to 85 ℃ in a stirring state, stirring until dissolution is complete, adding chlorphenyl glycine ether, cooling after dissolution, and adding phase B for stirring and dissolution;

and 2) cooling to 45 ℃, adding the C-phase raw material, stirring until the C-phase raw material is uniformly dispersed, and discharging to obtain the peony essence.

Application examples 7 to 8

The composition and the dosage (unit Kg) of the peony shampoo are shown in Table 9.

TABLE 9

The preparation method of the peony shampoo comprises the following steps:

step 1), adding deionized water and guar hydroxypropyl trimethyl ammonium chloride into a main pot, adding citric acid in a stirring state, heating to 80 ℃ after dissolving and transparency, adding ammonium lauryl sulfate and sodium lauryl ether sulfate, stirring until dissolving and transparency, adding the rest raw materials of phase A, and stirring until complete dissolving and cooling.

Step 2), the temperature is reduced to 60 ℃, the B phase raw material is added, and the mixture is stirred at a low speed until the mixture is completely dissolved and dispersed;

and 3) cooling to 45 ℃, sequentially adding the C-phase raw materials, slowly stirring until the C-phase raw materials are completely dissolved, and discharging to obtain the peony shampoo.

The present embodiment is merely illustrative of the present application and is not intended to be limiting, and those skilled in the art, after having read the present specification, may make modifications to the present embodiment without creative contribution as required, but is protected by patent laws within the scope of the claims of the present application.

Claims

1. A preparation method of a peony compound soothing factor is characterized by comprising the following steps: the method comprises the following steps:

step 6), flocculating the second filtrate, centrifuging and taking supernatant;

2. The method for preparing the peony compound relief factor according to claim 1, which is characterized in that: the peony compound relief factor is prepared from the following components in percentage by mass:

component A:

peony flower: peony leaves= (8-10): 1, a step of;

component B:

the component A comprises the following components: component b=1: (1-3).

3. The method for preparing the peony compound relief factor according to claim 2, which is characterized in that: in the step 2), the preparation method of the adsorption material comprises the following steps:

4. The method for preparing the peony compound relief factor according to claim 3, wherein the method comprises the following steps: in the step 01), the mass ratio of the acrylic acid, acetonitrile, water and the ferroferric oxide nano particles is 4:64:100:5, a step of; the molar ratio of the target molecule, acrylic acid, N-methylene bisacrylamide and azodiisobutyronitrile is 3:22:6:6.

5. The method for preparing the peony compound relief factor according to claim 4, wherein the method comprises the following steps: in the step 2) of the preparation method of the peony compound relieving factor, the adsorption material is a compound of glycyrrhizic acid-adsorption material, paeonol-adsorption material and pine-red plum alkaloid A-adsorption material, and the mass ratio of the glycyrrhizic acid-adsorption material to the paeonol-adsorption material to the pine-red plum alkaloid A-adsorption material is 1:5:1.5.

6. the method for preparing the peony compound relief factor according to any one of claims 1-5, wherein the method comprises the following steps: in the step 2) of the preparation method of the peony composite soothing factor, the volume ratio of the input adsorption material to the input component B is 1:1-3, and the extraction time is 2-3h.

7. The method for preparing the peony compound relief factor according to claim 6, wherein the method is characterized by comprising the following steps: in the step 2) of the preparation method of the peony compound relieving factor, during the cyclic extraction, the cyclic extraction is formed from bottom to top, and the medicinal material particles are controlled to be in an expanded bed state for the cyclic extraction.

8. The method for preparing the peony compound relief factor according to claim 6, wherein the method is characterized by comprising the following steps: in the step 4) and the step 7) of the preparation method of the peony compound relieving factor, the eluent is the compounding of the peony hydrosol prepared in the step 1) and the 1,3 butanediol, and the mass ratio of the peony hydrosol to the 1,3 butanediol is 4:6.

9. The method for preparing the peony compound relief factor according to claim 6, wherein the method is characterized by comprising the following steps: in the step 8) of the preparation method of the peony compound soothing factor, bifidobacterium longum is adopted as a strain during fermentation, and anaerobic fermentation is performed.

10. A peony compound relieving factor is characterized in that: is prepared by the preparation method of the peony compound relief factor according to any one of claims 1-9.

11. Use of a peony complex relief factor according to claim 10, wherein: is added into personal care products to prepare the personal care products containing the peony compound soothing factors.

12. Use of a peony complex relief factor according to claim 10, wherein: can be added into food or health product to prepare food or health product containing peony compound relieving factor.

13. Use of a peony complex relief factor according to claim 10, wherein: is added into medicines to prepare medicines containing peony compound relief factors.