CN1162645A - Method for producing compounded amino acids by hologenzymes process from beer yeast - Google Patents

Abstract

A enzymolysis process for extracting compound amino acids from beer yeast includes such steps as reducing acidity of raw yeast, deactivating, hydrolyzing, press filtering concentrating, decolouring, filtering and spraying, and features gentle condition, sufficient wall breaking, high output rate and low cost. Its product has the advantages of reasonable contents of 8 necessary amino acids, complete components and high quality.

Description

The method of producing compounded amino acids by hologenzymes process from beer yeast

The present invention relates to the technology that enzyme process prepares organic compounds containing nitrogen, specifically a kind of enzyme broken wall method of utilizing is extracted the method that the yeast intracellular organic matter prepares products of amino acid.

Amino acid is the widely used nutritional enrichment additives of industry such as beverage, health care, medicine, food, cosmetic.At present, there are silkworm chrysalis, gelatin in its main raw material source.Preparation method has autolysis method, acid and alkali hydrolysis method, osmotic pressure ballistic method etc., and these method energy consumptions are big, the cost height, and also product purity is on the low side, and content is 20-40%.

The employing cereuisiae fermentum is a raw material, uses the mechanical means broken wall, as the experimental study of hydrolysis power report is arranged once with enzyme again." Zhejiang Polytechnic College journal " 93 year second phase P20-26, but do not see the record that the practicality processing method is arranged.General single zymin is difficult to the beta-glucan glycosides carbon bond that constitutes the tough and tensile cell walls of yeast is cut off, and it is abundant to be difficult to obtain enzymolysis, yield height, the product of quality purity.

The objective of the invention is to propose the practical cereuisiae fermentum enzyme process of a kind of industry and prepare method of compound amino-acid.It promptly is the full-enzyme method hydrolysis process of raw material with the cereuisiae fermentum.At first carry out the yeast deactivation again pair cell make the enzymolysis broken wall, carry out proteinic enzymolysis then, the processing condition gentleness, aminoacids content is abundant in the product, quality is pure, eight kinds of essential amino acid content are relatively near the reasonable proportioning structural requirement.

The method of producing compounded amino acids by hologenzymes process from beer yeast, its processing step is followed successively by:

1) raw material is handled: get fresh yeast and carry out press filtration, meet the requirements of pH value with the clear water rinsing again, the acid adjustment degree is 6.0-7.0;

2) size mixing, deactivation: yeast and water are sized mixing in 1: 2 ratio, heat, deactivation 20-30 minute to 60-62 ℃;

3) hydrolysis: add mixing enzyme preparation, consumption is the 1-2% of yeast slurry raw material, comprising proteolytic enzyme is arranged, α-Dian Fenmei, beta-glucanase mixes, stirred in four hours once, under 40-50 ℃ of temperature, be hydrolyzed, the molecular weight that decomposes resolvent less than 3000 dalton till; Carry out then

4) press filtration, concentrate: hydrolyzed solution is carried out press filtration under 120 order filter clothes, filtrate enters concentrating pan, and to be evaporated to solid content be 20-30%, and vapor pressure is the 1-2 kg/cm, obtains to contain the compound amino acid primary liquid of growing smell.This stoste also can be used as the stoste goods and uses.

5) dust: carry out according to a conventional method, spissated compound amino acid primary liquid with the spray-dryer drying of dusting, is obtained the Powdered goods of faint yellow solid.

As a rule, can be in order to eliminate peculiar smell contained in the hydrolyzate in above-mentioned steps 4) increase a decolouring, deodorization step afterwards, promptly in stoste, add 100 purpose activated carbon 1-2%, stir, under 70-80 ℃ of temperature, handled 3-5 hour, use cotton cake filtering machine filtering activated carbon then, obtain removing the stoste behind the peculiar smell, remake the processing of dusting.

Full technical process from cereuisiae fermentum to the amino-acid powder finished product is:

Cereuisiae fermentum-squeeze-wash-size mixing-deactivation-hydrolysis-press filtration-concentrate-decolour-filter-dust-pack-dispatch from the factory.

The goods that the inventive method makes are rich in 18 seed amino acids, and the utilization ratio height of protein and ammonia-state nitrogen also can reach the experimental level in the documents under enlarging working condition.Protein yield 〉=80%, ammonia-state nitrogen yield 〉=50%.Eight kinds of indispensable amino acids meet the FAO/WHO pattern requirement that UNO (United Nations Organization) proposes substantially.Can satisfy the requirement that cereuisiae fermentum is realized abundant broken wall with the full-enzyme method hydrolysis.The setting of processing condition is as the criterion with the degree of control hydrolysis small peptide molecular weight, and is proper, practical to determining the hydrolysis terminal point, reached the technology purpose.Because hydrolysis time is too short, protein decomposes insufficient, and polymerization degree height influences yield and quality, also is not easy to the drying of dusting, and hydrolysis time is long, easily causes living contaminants, influences the product mouthfeel, and plant factor is reduced.From quality, component content, production cost aspect relatively, all be better than the production of traditional abundant amino acids from silkworm pupa.

In addition, can realize decolouring and deodorizing, reach the grade aspect and further require and effect goods with the activated carbon deodorization process.

Table 1 has been listed the test result of the aminoacids content of a sample of amino-acid powder.Aminoacids content ratio height wherein, kind is also very abundant.

Table 2 is listed eight kinds of main aminoacids content compositional models producing from cereuisiae fermentum and is compared.Table 1: the aminoacids content test data of sample

Sequence number	Test subject		Measure unit	Sample number	Assay
						??1	Amino-acid nitrogen		????g/100g		????8.2
??2	Amino acid	Aspartic acid (ASP)	????mg/100g		????7390.36
						Threonine (THR)	????mg/100g		????3621.90
		Serine (SER)	????mg/100g		????2249.70
						L-glutamic acid (GLU)	????mg/100g		????7579.40
		Proline(Pro) (PRO)	????mg/100g		Do not survey
						Glycine (GLA)	????mg/100g		????5915.15
		L-Ala (ALA)	????mg/100g		????6746.78
						Gelucystine (CYS)	????mg/100g		????950.49
		Xie Ansuan (VAL)	????mg/100g		????5178.90
						The r-butyric acid	????mg/100g		????197.40
		Methionine(Met) (MET)	????mg/100g		????1599.60
						Isoleucine (ILE)	????mg/100g		????3416.77
		Leucine (LEU)	????mg/100g		????4983.41
						Tyrosine (TYR)	????mg/100g		????5230.49
		L-Ala (PHE)	????mg/100g		????8523.72
						Histidine (HIS)	????mg/100g		????1593.47
		Methionin (LYS)	????mg/100g		????4624.00
						Ammonia (NH3)	????-------		????-------
		Arginine (ARG)	????mg/100g		????2413.01
						Tryptophane (TRY)	????mg/100g		????202.13
		??3	Total amino acid content		????mg/100g						????72416.68

Table 2: essential amino acids content and standard are relatively in the cereuisiae fermentum goods

Essential amino acids content g/100g bak protein source	Methionin	Threonine	Methyl-sulfuric acid ammonia	Xie Ansuan	Isoleucine	Leucine	Phenylalanine	Tryptophane
									The FAO/WHO reference standard	???4.3	???2.8	???4.3	????4.3	????4.3	?????4.9	????2.9	????1.0
Cereuisiae fermentum	???6.4	???5.2	???3.4	????5.4	????5.6	?????6.8	????4.2	????1.2

Embodiment 1:

With the fresh yeast in the beer production, impurity elimination, with the plate-and-frame filter press press filtration of being furnished with 100 order filter clothes, get its 500 kilograms of yeast slurrys, pass to about 40 minutes of deionized water (or clear water) rinsing in the press filtration, to pH value be 6.5; The yeast slurry filter cake is smashed to pieces and with 1 ton of deionized water mixing, is pumped into the deactivation pot, and adding preservative agent 1 kilogram of Tegosept E fourth fat and 75 gram zinc chloride can guarantee the steady quality in the technological process and play activation, stir evenly, in 60 ℃ of deactivations 25 minutes; In the yeast feed liquid, add 6 kilograms of mixing enzyme preparations, comprising proteolytic enzyme (500-700u), α-Dian Fenmei (5000-7000u), beta-glucanase (200u), about 2: 1: 2 of ratio is under 48 ± 1 ℃ of temperature, about 48 hours of hydrolysis detects the small peptide molecular weight less than 3000 dalton; Filtering and concentrating obtains about 500 kilograms of amino acid primary liquids then, and including solid substance is 20-25%.Stoste can be directly used in the later stage packing and be made as liquor.

Embodiment 2:

Will be as embodiment 1, the amino acid primary liquid that makes adds 6 kilogram of 100 purpose activated carbon therein, mixes thoroughly, is warmed to 75 ℃ of insulations and decolours and deodorize 4 hours; Filter with cottonseed cake then and obtain clear liquid stoste.Stoste can be directly used in the later stage packing and be made as liquor, or the adding vehicle is made paste.

Embodiment 3:

Make stoste after the deodorizing as embodiment 1,2, make about 50 kilograms of faint yellow solid powder pulvis with the 50 type spray-dryers drying of dusting.The import wind-warm syndrome is 230 ℃ when dusting, and the outlet wind-warm syndrome is 90 ℃, and the pulvis goods can be directly used in packing or post-production, make the goods of different dosage form.

Claims (4)

1, a kind of method of producing compounded amino acids by hologenzymes process from beer yeast, wherein processing step is followed successively by:

1) raw material is handled: meet the requirements of pH value with the clear water rinsing after getting the fresh yeast press filtration, the acid adjustment degree is 6.0-7.0;

2) size mixing, deactivation: yeast and water are sized mixing in 1: 2 ratio, heat, deactivation 20-30 minute to 60-62 ℃;

3) hydrolysis: add mixing enzyme preparation, consumption is the 1-2% of yeast slurry raw material, comprising proteolytic enzyme is arranged, α-Dian Fenmei, beta-glucanase mixes, stirred in four hours once, under 40-50 ℃ of temperature, be hydrolyzed, the molecular weight that decomposes resolvent less than 3000 dalton till; Carry out then

4) press filtration, concentrate: hydrolyzed solution is carried out press filtration under 120 order filter clothes, filtrate enters concentrating pan, and to be evaporated to solid content be 20-30%, and vapor pressure is the 1-2 kg/cm, obtains to contain the compound amino acid primary liquid of growing smell;

5) dust: carry out according to a conventional method, spissated compound amino acid primary liquid with the spray-dryer drying of dusting, is obtained the Powdered goods of faint yellow solid.

2, the method for claim 1, step 4) and 5 wherein) also is added with a decolouring, deodorization step between: in concentrating stoste, add 100 purpose activated carbon 1-2%, stir, under 70-80 ℃ of temperature, handled 3-5 hour, use cotton cake filtering machine filtering activated carbon then.

3, the method for claim 1, wherein step 2) in also be added with sanitas Tegosept E fourth fat 0.2-0.3% and zinc chloride to 150-180PPM.

4, the method for claim 1, wherein the optimum amount of mixed enzyme is in the step 3): proteolytic enzyme (500-700u): α-Dian Fenmei (5000-7000u): beta-glucanase (200u)=2: 1: 2.