CN116250809B - Ultrasonic nerve regulation target area positioning device and method - Google Patents
Ultrasonic nerve regulation target area positioning device and method Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0093—Detecting, measuring or recording by applying one single type of energy and measuring its conversion into another type of energy
- A61B5/0095—Detecting, measuring or recording by applying one single type of energy and measuring its conversion into another type of energy by applying light and detecting acoustic waves, i.e. photoacoustic measurements
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0033—Features or image-related aspects of imaging apparatus classified in A61B5/00, e.g. for MRI, optical tomography or impedance tomography apparatus; arrangements of imaging apparatus in a room
- A61B5/004—Features or image-related aspects of imaging apparatus classified in A61B5/00, e.g. for MRI, optical tomography or impedance tomography apparatus; arrangements of imaging apparatus in a room adapted for image acquisition of a particular organ or body part
- A61B5/0042—Features or image-related aspects of imaging apparatus classified in A61B5/00, e.g. for MRI, optical tomography or impedance tomography apparatus; arrangements of imaging apparatus in a room adapted for image acquisition of a particular organ or body part for the brain
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/01—Measuring temperature of body parts ; Diagnostic temperature sensing, e.g. for malignant or inflamed tissue
- A61B5/015—By temperature mapping of body part
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/48—Other medical applications
- A61B5/4836—Diagnosis combined with treatment in closed-loop systems or methods
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/48—Other medical applications
- A61B5/4887—Locating particular structures in or on the body
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N7/00—Ultrasound therapy
- A61N7/02—Localised ultrasound hyperthermia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/40—Animals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/42—Evaluating a particular growth phase or type of persons or animals for laboratory research
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N7/00—Ultrasound therapy
- A61N2007/0004—Applications of ultrasound therapy
- A61N2007/0021—Neural system treatment
Abstract
The application discloses ultrasonic nerve regulation and control target area positioning device and method, the device includes: the photoacoustic excitation module is used for focusing pulse laser generated by the pulse laser on a target biological sample through the two-dimensional scanning component to generate a target photoacoustic signal; the ultrasonic nerve control component generates focused ultrasonic waves, and transmits the focused ultrasonic waves to the target biological sample so as to heat up a target area of the target biological sample; the infrared thermal imaging component is used for collecting infrared thermal radiation emitted by a target biological sample to obtain a thermal radiation signal; the signal processing module is used for performing signal processing operation on the target photoacoustic signal to obtain an optimized photoacoustic signal; and the analysis control module is used for generating a thermal radiation distribution diagram according to the thermal radiation signal, adjusting the target area of the target biological sample scanned by the two-dimensional scanning component based on the thermal radiation distribution diagram, and constructing a target photoacoustic image according to the optimized photoacoustic signal. The method and the device can solve the technical problem that the efficiency of the scanning process is too low and the positioning speed is influenced in the prior art.
Description
Technical Field
The application relates to the technical field of photoacoustic imaging, in particular to an ultrasonic nerve regulation target area positioning device and method.
Background
Ultrasonic nerve regulation is to guide ultrasonic wave to focus in corresponding target area of cranium and form energy convergence in the focused target area, so as to improve various brain disease symptoms. The high-intensity focused ultrasound can be classified into high-intensity focused ultrasound and low-intensity focused ultrasound according to different ultrasonic energy, wherein the high-intensity focused ultrasound utilizes a thermal effect generated by ultrasonic energy aggregation to thermally ablate local tissues, and the low-intensity focused ultrasound utilizes a mechanical effect or cavitation phenomenon of ultrasound to achieve a therapeutic purpose. However, because the skull of the brain has a complex structure, the phase of the wave front of the ultrasonic wave changes, so that the ultrasonic wave is difficult to accurately reach a treatment target area, common positioning modes include hydrophone implantation, fluorescence imaging, tissue slice, nuclear magnetic resonance imaging and the like, but the hydrophone implantation and tissue slice modes are invasive, contrast agent is needed to be used in fluorescence imaging, imaging has no depth information, nuclear magnetic resonance imaging is expensive, and experimental research is not facilitated.
The basic principle of the photoacoustic microscopic imaging technology is photoacoustic effect, imaging speed is high, and meanwhile depth information of a measured object can be obtained. However, when the target region is used for positioning, the specific position of the target region is not known, so that the whole imaging region is scanned, and the non-target region position is also scanned, and the positioning speed is greatly reduced by the scanning mode.
Disclosure of Invention
The application provides an ultrasonic nerve regulation and control target area positioning device and method, which are used for solving the technical problem that the efficiency of a scanning process in the prior art is too low and the target area positioning speed is influenced.
In view of this, the first aspect of the present application provides an ultrasonic neuromodulation target positioning device, comprising: the device comprises a photoacoustic excitation module, an ultrasonic nerve regulation and control assembly, an infrared thermal imaging component, a signal processing module and an analysis control module;
the photoacoustic excitation module comprises a pulse laser and a two-dimensional scanning component, and is used for focusing pulse laser generated by the pulse laser on a target biological sample through the two-dimensional scanning component to generate a target photoacoustic signal;
the ultrasonic nerve control component generates focused ultrasonic waves and transmits the focused ultrasonic waves to a target biological sample to realize ultrasonic control so as to heat a target area of the target biological sample;
the infrared thermal imaging component comprises a thermal imager and is used for collecting infrared thermal radiation emitted by the target biological sample to obtain a thermal radiation signal;
the signal processing module is used for performing signal processing operation on the target photoacoustic signal to obtain an optimized photoacoustic signal, and the signal processing operation comprises amplification processing;
the analysis control module is used for generating a thermal radiation distribution diagram according to the thermal radiation signal, adjusting the two-dimensional scanning component to scan the target area of the target biological sample based on the thermal radiation distribution diagram, and constructing a target photoacoustic image according to the optimized photoacoustic signal.
Preferably, the photoacoustic excitation module further includes: the device comprises a filtering collimation assembly, a field lens and a voltage output module;
the pulse laser, the filtering collimation component, the two-dimensional scanning component and the field lens are coaxially arranged;
the voltage output module is used for receiving a control instruction of a computer, triggering a vibrating mirror of the two-dimensional scanning component to shape the pulse laser based on the control instruction, forming a focusing light beam by combining the field lens, focusing on the target biological sample, and generating a target photoacoustic signal.
Preferably, the signal processing module comprises a transparent ultrasonic transducer, a water tank, a signal amplifier and a data acquisition card;
the transparent ultrasonic transducer is used for converting the target photoacoustic signal into a target electrical signal;
the signal amplifier is used for amplifying the target electric signal;
the data acquisition card is used for converting the amplified target electric signal into a digital signal to obtain an optimized photoacoustic signal.
Preferably, the transparent ultrasonic transducer is immersed in the water tank and is located directly below the two-dimensional scanning component;
the transparent ultrasonic transducer, the signal amplifier and the data acquisition card are electrically connected in sequence.
Preferably, a square hole is formed in the bottom of the water tank, and deionized water is filled in the water tank to serve as a coupling agent;
the square holes are sealed by a single-layer transparent film.
Preferably, the ultrasonic nerve modulation and control component comprises an ultrasonic transducer, an acoustic holographic lens and an acoustic reflector;
the ultrasonic transducer, the acoustic holographic lens and the acoustic reflector are coaxially arranged, and the acoustic reflector is positioned between the two-dimensional scanning component and the transparent ultrasonic transducer.
Preferably, the analysis control module is specifically configured to:
generating a thermal radiation profile from the thermal radiation signal;
determining target coordinates of the target biological sample based on the thermal radiation profile;
adjusting the two-dimensional scanning component to perform local scanning on the target area of the target biological sample according to the target area coordinates;
and constructing a target photoacoustic image according to the optimized photoacoustic signal.
The second aspect of the application provides a method for positioning an ultrasonic nerve regulation target area, which comprises the following steps:
projecting the pulse laser generated by the pulse laser to a target biological sample through a two-dimensional scanning component;
transmitting ultrasonic waves generated by an ultrasonic transducer to the target biological sample, so that the target area temperature of the target biological sample is increased;
collecting infrared thermal radiation emitted by the target biological sample to obtain a thermal radiation signal;
adjusting the two-dimensional scanning component to locally scan the target area of the target biological sample based on the thermal radiation signal to generate a target photoacoustic signal;
and constructing a target photoacoustic image according to the target photoacoustic signal.
Preferably, the constructing a target photoacoustic image according to the target photoacoustic signal further includes:
and performing signal processing on the target photoacoustic signal, wherein the signal processing comprises amplification processing.
From the above technical solutions, the embodiments of the present application have the following advantages:
in this application, there is provided an ultrasonic nerve regulation and control target area positioner, including: the device comprises a photoacoustic excitation module, an ultrasonic nerve regulation and control assembly, an infrared thermal imaging component, a signal processing module and an analysis control module; the photoacoustic excitation module comprises a pulse laser and a two-dimensional scanning component, and is used for focusing pulse laser generated by the pulse laser on a target biological sample through the two-dimensional scanning component to generate a target photoacoustic signal; the ultrasonic nerve regulation and control component generates focused ultrasonic waves and transmits the focused ultrasonic waves to the target biological sample, so that ultrasonic regulation and control are realized, and the target area of the target biological sample is heated; the infrared thermal imaging component comprises a thermal imager and is used for collecting infrared thermal radiation emitted by a target biological sample to obtain a thermal radiation signal; the signal processing module is used for performing signal processing operation on the target photoacoustic signal to obtain an optimized photoacoustic signal, and the signal processing operation comprises amplification processing; and the analysis control module is used for generating a thermal radiation distribution diagram according to the thermal radiation signal, adjusting the target area of the target biological sample scanned by the two-dimensional scanning component based on the thermal radiation distribution diagram, and constructing a target photoacoustic image according to the optimized photoacoustic signal.
The utility model provides an ultrasonic nerve regulation and control target area positioner combines optoacoustic imaging and infrared imaging technique to image the biological sample of target, and ultrasonic nerve regulation and control subassembly promotes the temperature of the biological sample target area of target through the ultrasonic wave, and the infrared thermal radiation signal of sample after the intensification then can be gathered to the infrared thermal imaging appearance, can control two-dimensional scanning part based on this and scan the target area that infrared location was only put out, has removed the scanning time of non-target area part from, can improve target area location speed, and the depth information of sample can also be drawn to the optoacoustic imaging simultaneously, ensures the location reliability. Therefore, the method and the device can solve the technical problem that the efficiency of the scanning process in the prior art is too low and the target area positioning speed is affected.
Drawings
Fig. 1 is a schematic structural diagram of an ultrasonic nerve control target area positioning device according to an embodiment of the present application;
fig. 2 is a schematic flow chart of a method for positioning an ultrasonic nerve control target area according to an embodiment of the present application;
FIG. 3 is a schematic diagram of normalized photo-acoustic signal amplitude values at different temperatures according to an embodiment of the present application;
reference numerals:
a photoacoustic microimaging assembly 1; an infrared thermal imaging member 2; an ultrasonic nerve modulation control assembly 3; a signal processing module 4; pulsed lasers 1-1; a voltage output module 1-2; a filter collimation component 1-3; two-dimensional scanning means 1-4; 1-5 of field lens; 1-6 parts of a water tank; 1-7 parts of transparent ultrasonic transducer; 1-8 of an imaging table; an ultrasonic transducer 3-1; an acoustic holographic lens 3-2; an acoustic mirror 3-3; a signal amplifier 4-1; a data acquisition card 4-2; computer 4-3.
Detailed Description
In order to make the present application solution better understood by those skilled in the art, the following description will clearly and completely describe the technical solution in the embodiments of the present application with reference to the accompanying drawings in the embodiments of the present application, and it is apparent that the described embodiments are only some embodiments of the present application, not all embodiments. All other embodiments, which can be made by one of ordinary skill in the art without undue burden from the present disclosure, are within the scope of the present disclosure.
For ease of understanding, referring to fig. 1, an embodiment of an ultrasonic nerve modulation target positioning device provided in the present application includes: the device comprises a photoacoustic excitation module, an ultrasonic nerve regulation and control assembly 3, an infrared thermal imaging component 2, a signal processing module 4 and an analysis control module.
The photoacoustic excitation module comprises a pulse laser 1-1 and a two-dimensional scanning component 1-4, and is used for focusing pulse laser generated by the pulse laser 1-1 on a target biological sample through the two-dimensional scanning component 1-4 to generate a target photoacoustic signal.
Further, the photoacoustic excitation module further includes: the device comprises a filtering collimation component 1-3, a field lens 1-5 and a voltage output module 1-2;
the pulse laser 1-1, the filtering collimation component 1-3, the two-dimensional scanning component 1-4 and the field lens 1-5 are coaxially arranged;
the voltage output module 1-2 is used for receiving a control instruction of the computer 4-3, triggering a vibrating mirror of the two-dimensional scanning component 1-4 to shape pulse laser based on the control instruction, forming a focusing light beam by combining the field lens 1-5, focusing on a target biological sample, and generating a target photoacoustic signal.
The photoacoustic excitation module is a front-stage laser emission part in the photoacoustic microscopic imaging assembly 1, and the specific working principle is as follows: pulse laser is sent out through a pulse laser 1-1, then the pulse laser is subjected to filtering collimation operation through a filtering collimation component 1-3, then a control instruction of a computer 4-3 is received through a voltage output module 1-2, a vibrating mirror of a two-dimensional scanning component 1-4 is triggered to deflect the laser based on the control instruction, and a focusing beam is formed through a field lens 1-5 to be focused on a target biological sample, so that a target photoacoustic signal is generated. The voltage output module is used for controlling the galvanometer scanning and adjusting the laser, and can regulate and control the time sequence of actions of different devices in the device to ensure the time sequence synchronization.
The ultrasonic nerve control assembly 3 generates focused ultrasonic waves and transmits the focused ultrasonic waves to the target biological sample, so that ultrasonic control is realized, and the target area of the target biological sample is heated.
Further, the ultrasonic nerve control component 3 comprises an ultrasonic transducer 3-1, an acoustic holographic lens 3-2 and an acoustic reflector 3-3;
the ultrasonic transducer 3-1, the acoustic holographic lens 3-2 and the acoustic reflector 3-3 are coaxially arranged, and the acoustic reflector 3-3 is positioned between the two-dimensional scanning component 1-4 and the transparent ultrasonic transducer 1-7.
It should be noted that the amplitude of the photoacoustic signal is proportional to the density of the absorbed optical energy, and the coefficient is called the grineisen parameter, which depends on the volume expansion coefficient and the speed of sound, both of which are temperature dependent and quasi-linearly proportional to the pre-pulse temperature. Thus, the glinide parameter is linear with the pre-pulse temperature (T) over a physiological temperature range. When external factors cause local transient temperature increases, the local gardneson parameter increases within a thermally limited time (i.e., the time before local heat diffuses out), which is known as the gardneson relaxation effect. The ultrasonic nerve control assembly 3 is designed by utilizing the effect, ultrasonic irradiation is carried out on a target biological sample, and the local temperature of the target biological sample is changed, so that the infrared heat radiation distribution difference is enhanced, and accurate positioning is realized.
In addition, the signal amplifier 4-1, the data acquisition card 4-2 and the computer 4-3 are electrically connected in sequence, the infrared thermal imager 2 is electrically connected with the computer 4-3, and the voltage output module 1-2 is electrically connected with the pulse laser 1-1, the two-dimensional scanning component 1-4 and the data acquisition card 4-2. The pulse laser 1-1 in the embodiment selects a short pulse laser 1-1, the pulse width is smaller than 10ns, the working wavelength is 532nm, the single pulse energy is 7uJ, and the maximum scanning angle of the two-dimensional scanning component 1-4 is +/-20 degrees.
The working principle of the ultrasonic nerve control component 3 is as follows: the ultrasonic transducer 3-1 controlled by nerve emits ultrasonic wave, the focusing and position adjustment of the light beam are carried out through the acoustic holographic lens 3-2, and then the ultrasonic wave is irradiated on a corresponding target area of the sample to be controlled through the acoustic reflector 3-3. It will be appreciated that the acoustic mirror 3-3 has a relatively high transmission of the pulsed laser light emitted by the pulsed laser 1-1, substantially greater than 98%.
Moreover, the acoustic holographic lens 3-2 can focus the ultrasonic emitted by the ultrasonic transducer 3-1 at a required position on the sample to realize single probe regulation, wherein the focused ultrasonic can be reflected by the acoustic reflector 3-3; the ultrasound focus position can also be adjusted by changing the acoustic holographic lens 3-2. It will be appreciated that if the positioning image shows that there is a deviation in the ultrasound focus position, the adjustment is made by replacing the acoustic holographic lens 3-2.
The infrared thermal imaging unit 2 comprises a thermal imager for acquiring infrared thermal radiation emitted by a target biological sample to obtain a thermal radiation signal.
After the target biological sample is subjected to ultrasonic radiation, the temperature of a target area is higher; the infrared thermal imager can measure the distribution of the external red thermal radiation of the sample, and obtain thermal radiation signals with obvious temperature difference. Based on the heat radiation signals, the specific target area position can be determined, so that local scanning is realized, and low positioning efficiency caused by large-area scanning is avoided.
And the signal processing module 4 is used for performing signal processing operation on the target photoacoustic signal to obtain an optimized photoacoustic signal, wherein the signal processing operation comprises amplification processing.
Further, the signal processing module 4 comprises a transparent ultrasonic transducer 1-7, a water tank 1-6, a signal amplifier 4-1 and a data acquisition card 4-2;
a transparent ultrasonic transducer 1-7 for converting a target photoacoustic signal into a target electrical signal;
a signal amplifier 4-1 for amplifying the target electrical signal;
the data acquisition card 4-2 is used for converting the amplified target electric signal into a digital signal to obtain an optimized photoacoustic signal.
Further, the transparent ultrasonic transducer 1-7 is immersed in the water tank 1-6 and is positioned right below the two-dimensional scanning component 1-4;
the transparent ultrasonic transducer 1-7, the signal amplifier 4-1 and the data acquisition card 4-2 are electrically connected in sequence.
Further, square holes are formed in the bottoms of the water tanks 1-6, and deionized water is filled in the water tanks 1-6 to serve as a coupling agent;
the square holes are sealed by a single layer transparent film.
It should be noted that, the signal processing module 4 is mainly configured to receive the photoacoustic signal and perform some signal processing on the photoacoustic signal, so as to facilitate subsequent image construction. It is clear that the transparent ultrasonic transducer 1-7 is in the water tank 1-6 and is in communication connection with the signal amplifier 4-1 and the data acquisition card 4-2. The square holes at the bottom of the water tanks 1-6 are sealed by transparent films, and target biological samples are attached to the outer parts of the transparent films, so that the scanning of the two-dimensional scanning components 1-4 can be received.
Furthermore, in the present embodiment, the transparent ultrasonic transducers 1 to 7 have a transparent LiNbO3 single crystal as a substrate, a transparent epoxy resin as an acoustic matching layer, and an external dimension of 33X 33mm 2 The window size is 22X 22mm 2 The height is 10mm and the center frequency is 10MHz.
Specifically, the transparent ultrasonic transducer 1-7 converts the photoacoustic signal into an electrical signal, the electrical signal is amplified by the signal amplifier 4-1 and then is transmitted to the data acquisition card 4-2, the data acquisition card 4-2 converts the electrical signal into a digital signal and stores the digital signal, or the digital signal is transmitted to the analysis control module in the computer 4-3 for analysis, and a photoacoustic image of a corresponding target area is formed.
And the analysis control module is used for generating a thermal radiation distribution diagram according to the thermal radiation signals, adjusting the two-dimensional scanning component 1-4 to scan the target area of the target biological sample based on the thermal radiation distribution diagram, and constructing a target photoacoustic image according to the optimized photoacoustic signals.
Further, the analysis control module is specifically configured to:
generating a thermal radiation profile from the thermal radiation signal;
determining target coordinates of the target biological sample based on the thermal radiation profile;
the two-dimensional scanning component 1-4 is adjusted according to the coordinates of the target area to carry out local scanning on the target area of the target biological sample;
a target photoacoustic image is constructed from the optimized photoacoustic signal.
The thermal radiation distribution map generated according to the thermal radiation signals can clearly reflect the temperature distribution difference on the target biological sample, particularly the heated target area, so that the target area coordinates of the target biological sample can be determined based on the thermal radiation distribution map, then the two-dimensional scanning component 1-4 is adjusted according to the target area coordinates to only locally scan the target area, and then the collected target photoacoustic signals are generated only by the target area, so that the generated target photoacoustic image has more pertinence and the positioning speed is higher. It will be appreciated that the target coordinate information may be applied to the voltage output module 1-2, and the control instructions of the computer 4-3 may be received by the voltage output module 1-2, and the local scanning control may be performed on the two-dimensional scanning unit 1-4 based on the control instructions.
In the prior art, as the temperature of a focusing area is raised due to ultrasonic focusing, although the plane information of a measured object can be obtained rapidly by adopting an infrared thermal imaging method, the depth information of the measured object cannot be obtained. The basic principle of the photoacoustic microscopic imaging technology is that the photoacoustic effect is that when the photoacoustic microscopic imaging technology irradiates a biological tissue with a short pulse laser, the biological tissue absorbs pulse energy and rapidly expands to generate an ultrasonic signal, the photoacoustic signal is in a certain temperature range, the amplitude of the signal and the temperature are in positive linear correlation, the transparent ultrasonic transducer 1-7 is used for receiving the signals, and the corresponding biological tissue image can be obtained through the computer 4-3. The imaging mode has high resolution and imaging speed, and can obtain the depth information of the measured object. However, when the scanning device is used for positioning, the specific position of the target area is not known, so that the whole imaging area is scanned, the non-target area position is also scanned, and the positioning speed is greatly reduced by the scanning mode, so that the efficiency is poor. Therefore, the embodiment provides the positioning device, which not only can extract the depth information of the target biological sample, but also can accurately and rapidly scan the target area position of the sample to obtain the target photoacoustic image.
According to the ultrasonic nerve regulation and control target area positioning device, the photoacoustic imaging and infrared imaging technology is combined to image the target biological sample, the ultrasonic nerve regulation and control assembly 3 improves the temperature of the target area of the target biological sample through ultrasonic waves, the infrared thermal imager can collect infrared thermal radiation signals of the sample after temperature rise, based on the infrared thermal radiation signals, the two-dimensional scanning component 1-4 can be controlled to scan only the target area positioned by infrared, scanning time of a non-target area part is saved, the target area positioning speed can be improved, meanwhile, the photoacoustic imaging can also extract depth information of the sample, and positioning reliability is ensured. Therefore, the embodiment of the application can solve the technical problems that the scanning process in the prior art is low in efficiency and the target area positioning speed is influenced.
For ease of understanding, referring to fig. 2, the present application provides an embodiment of a method for positioning an ultrasound neuromodulation target region, comprising:
step 201, projecting pulse laser generated by a pulse laser to a target biological sample through a two-dimensional scanning component;
step 202, transmitting ultrasonic waves generated by an ultrasonic transducer to a target biological sample, so that the temperature of a target area of the target biological sample is increased;
step 203, collecting infrared thermal radiation emitted by a target biological sample to obtain a thermal radiation signal;
step 204, adjusting the two-dimensional scanning component to locally scan the target area of the target biological sample based on the thermal radiation signal, and generating a target photoacoustic signal;
step 205, constructing a target photoacoustic image according to the target photoacoustic signal.
Further, step 205, further includes:
and performing signal processing on the target photoacoustic signal, wherein the signal processing comprises amplification processing.
It should be noted that some preparation work is needed before the laser emission, the experimental mice after dehairing and head epidermis stripping treatment are placed on the imaging tables 1-8, deionized water is smeared on the parts to be imaged as a coupling agent, and the deionized water is tightly attached to the transparent sealing film of the water tank.
After the preparation work is finished, ultrasonic waves can be sent out through the nerve control ultrasonic transducer to pass through the acoustic holographic lens, the acoustic holographic lens adjusts and focuses the acoustic beams to irradiate the brain of the experimental mouse through the acoustic reflector, and the irradiated part absorbs the energy of the ultrasonic waves to rise in temperature. Then, the infrared thermal imager absorbs infrared thermal radiation signals of the to-be-imaged part of the small animal, an infrared thermal radiation energy distribution diagram of the to-be-imaged part is formed by a computer after processing, and the plane position of the regulation target area can be obtained through the energy distribution diagram. Then, the scanning area of the photoacoustic microimaging assembly 1 can be positioned, a pulse laser emits pulse laser, the laser passes through a filtering collimation module, a two-dimensional scanning module and a field lens, the filtered, collimated and focused light beam passes through a transparent ultrasonic transducer to irradiate the surface of a sample and generate a photoacoustic signal, the photoacoustic signal generated by the sample is received by the transparent ultrasonic transducer and then is converted into an electric signal, the electric signal is amplified by a signal amplifier and then is transmitted into a data acquisition card, the data acquisition card converts the electric signal into a digital signal and transmits the digital signal to a computer, and the corresponding target area image is finally obtained through analysis and processing of the computer. And finally, waiting for the temperature of the imaging part to be reduced to the normal temperature, acquiring the target photoacoustic image again, and comparing the two obtained photoacoustic images to obtain the depth information of the part to be imaged.
It will be appreciated that after the ultrasonic energy used for neuromodulation is absorbed by the target region, the temperature of the target region will rise, and thus the infrared thermal radiation energy will also rise, and it will be seen from the energy profile that the energy at the target region is significantly higher than in other regions.
In general, since the specific target area position regulated by ultrasonic nerves is not known, the whole brain area needs to be imaged in order to locate the target area; the infrared thermal imager can rapidly determine the plane position of the target area, the acquisition component only scans the local small area, so that the depth information of the area is obtained, and the plane information is combined to obtain three-dimensional target area information, so that the target area is accurately positioned.
It should be noted that, in the embodiment of the device provided in the present application, besides the above method for realizing target area positioning, an exogenous contrast agent may be injected into a blood vessel at the tail of an experimental mouse, the contrast agent flows along with blood to the cerebral cortex of the mouse, and the device is adopted to image the brain of the mouse, so that the injected contrast agent in the embodiment promotes the positive correlation between the amplitude and the temperature of the photoacoustic signal, and under the same temperature condition, please refer to fig. 3, the amplitude of the photoacoustic signal obtained in the embodiment is higher, and the imaging quality is better. Therefore, when the target area is positioned and the used contrast agent does not damage the experimental sample, the imaging quality can be further improved by using the contrast agent. Specific imaging procedures may be referred to the above description and will not be repeated here.
In the several embodiments provided in this application, it should be understood that the disclosed apparatus and method may be implemented in other ways. For example, the apparatus embodiments described above are merely illustrative, e.g., the division of the units is merely a logical function division, and there may be additional divisions when actually implemented, e.g., multiple units or components may be combined or integrated into another system, or some features may be omitted or not performed. Alternatively, the coupling or direct coupling or communication connection shown or discussed with each other may be an indirect coupling or communication connection via some interfaces, devices or units, which may be in electrical, mechanical or other form.
The units described as separate units may or may not be physically separate, and units shown as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the units may be selected according to actual needs to achieve the purpose of the solution of this embodiment.
In addition, each functional unit in each embodiment of the present application may be integrated in one processing unit, or each unit may exist alone physically, or two or more units may be integrated in one unit. The integrated units may be implemented in hardware or in software functional units.
The integrated units, if implemented in the form of software functional units and sold or used as stand-alone products, may be stored in a computer readable storage medium. Based on such understanding, the technical solution of the present application may be embodied in essence or a part contributing to the prior art or all or part of the technical solution in the form of a software product stored in a storage medium, including several instructions to execute all or part of the steps of the methods described in the embodiments of the present application by a computer device (which may be a personal computer, a server, or a network device, etc.). And the aforementioned storage medium includes: u disk, mobile hard disk, read-Only Memory (ROM), random access Memory (Random Access Memory, RAM), magnetic disk or optical disk, etc.
The above embodiments are merely for illustrating the technical solution of the present application, and not for limiting the same; although the present application has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Claims (6)
1. An ultrasonic neuromodulation target area positioning device, comprising: the device comprises a photoacoustic excitation module, an ultrasonic nerve regulation and control assembly, an infrared thermal imaging component, a signal processing module and an analysis control module;
the photoacoustic excitation module comprises a pulse laser and a two-dimensional scanning component, and is used for focusing pulse laser generated by the pulse laser on a target biological sample through the two-dimensional scanning component to generate a target photoacoustic signal;
the ultrasonic nerve regulation and control component generates focused ultrasonic waves, transmits the focused ultrasonic waves to a target biological sample, realizes ultrasonic regulation and control, and heats a target area of the target biological sample, and comprises an ultrasonic transducer, an acoustic holographic lens and an acoustic reflector;
the ultrasonic transducer, the acoustic holographic lens and the acoustic reflector are coaxially arranged, and the acoustic reflector is positioned between the two-dimensional scanning component and the transparent ultrasonic transducer;
the infrared thermal imaging component comprises a thermal imager and is used for collecting infrared thermal radiation emitted by the target biological sample to obtain a thermal radiation signal;
the signal processing module is used for performing signal processing operation on the target photoacoustic signal to obtain an optimized photoacoustic signal, the signal processing operation comprises amplification processing, and the signal processing module comprises the transparent ultrasonic transducer, a water tank, a signal amplifier and a data acquisition card;
the transparent ultrasonic transducer is used for converting the target photoacoustic signal into a target electrical signal;
the signal amplifier is used for amplifying the target electric signal;
the data acquisition card is used for converting the amplified target electric signal into a digital signal to obtain an optimized photoacoustic signal;
the analysis control module is used for generating a thermal radiation distribution diagram according to the thermal radiation signal, adjusting the two-dimensional scanning component to scan the target area of the target biological sample based on the thermal radiation distribution diagram, and constructing a target photoacoustic image according to the optimized photoacoustic signal, and is specifically used for:
generating a thermal radiation profile from the thermal radiation signal;
determining target coordinates of the target biological sample based on the thermal radiation profile;
adjusting the two-dimensional scanning component to perform local scanning on the target area of the target biological sample according to the target area coordinates;
and constructing a target photoacoustic image according to the optimized photoacoustic signal.
2. The ultrasonic neuromodulation target location device as in claim 1, wherein the photoacoustic excitation module further comprises: the device comprises a filtering collimation assembly, a field lens and a voltage output module;
the pulse laser, the filtering collimation component, the two-dimensional scanning component and the field lens are coaxially arranged;
the voltage output module is used for receiving a control instruction of a computer, triggering a vibrating mirror of the two-dimensional scanning component to shape the pulse laser based on the control instruction, forming a focusing light beam by combining the field lens, focusing on the target biological sample, and generating a target photoacoustic signal.
3. The ultrasonic neuromodulation target location device as in claim 1, wherein the transparent ultrasonic transducer is immersed in the water tank and directly below the two-dimensional scanning component;
the transparent ultrasonic transducer, the signal amplifier and the data acquisition card are electrically connected in sequence.
4. The ultrasonic nerve control target area positioning device according to claim 1, wherein a square hole is formed in the bottom of the water tank, and deionized water is filled in the water tank as a coupling agent;
the square holes are sealed by a single-layer transparent film.
5. An ultrasonic neuromodulation target location method for use with the apparatus of any of claims 1-4, comprising:
projecting the pulse laser generated by the pulse laser to a target biological sample through a two-dimensional scanning component;
transmitting ultrasonic waves generated by an ultrasonic transducer to the target biological sample, so that the target area temperature of the target biological sample is increased;
collecting infrared thermal radiation emitted by the target biological sample to obtain a thermal radiation signal;
adjusting the two-dimensional scanning component to locally scan the target area of the target biological sample based on the thermal radiation signal to generate a target photoacoustic signal;
and constructing a target photoacoustic image according to the target photoacoustic signal.
6. The method of claim 5, wherein constructing a target photoacoustic image from the target photoacoustic signals further comprises:
and performing signal processing on the target photoacoustic signal, wherein the signal processing comprises amplification processing.
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