CN116223457A - 分析多种荧光团的混合荧光响应的方法和荧光分析仪 - Google Patents
分析多种荧光团的混合荧光响应的方法和荧光分析仪 Download PDFInfo
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- CN116223457A CN116223457A CN202211581542.3A CN202211581542A CN116223457A CN 116223457 A CN116223457 A CN 116223457A CN 202211581542 A CN202211581542 A CN 202211581542A CN 116223457 A CN116223457 A CN 116223457A
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
- G01J3/4406—Fluorescence spectrometry
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
- G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
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- G02B21/365—Control or image processing arrangements for digital or video microscopes
- G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
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Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP21212550.4A EP4191234B1 (en) | 2021-12-06 | 2021-12-06 | Method of analysing a mixed fluorescence response of a plurality of fluorophores, fluorescence analyser, fluorescence microscope and computer program |
| EP21212550.4 | 2021-12-06 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116223457A true CN116223457A (zh) | 2023-06-06 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211581542.3A Pending CN116223457A (zh) | 2021-12-06 | 2022-12-05 | 分析多种荧光团的混合荧光响应的方法和荧光分析仪 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US12339229B2 (enExample) |
| EP (1) | EP4191234B1 (enExample) |
| JP (1) | JP2023084119A (enExample) |
| CN (1) | CN116223457A (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116879250A (zh) * | 2023-07-07 | 2023-10-13 | 宁波力显智能科技有限公司 | 荧光显微成像方法、装置、计算机设备和存储介质 |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR3117595B1 (fr) * | 2020-12-16 | 2023-10-06 | Commissariat Energie Atomique | Dispositif compact de caractérisation d’une substance photoluminescente |
| EP4293408A1 (en) * | 2022-06-14 | 2023-12-20 | Leica Microsystems CMS GmbH | Imaging device and method for aligning images |
| US20240273678A1 (en) * | 2023-01-30 | 2024-08-15 | The Research Foundation For The State University Of New York | Unmixing image data |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6687000B1 (en) * | 2000-06-26 | 2004-02-03 | Wisconsin Alumni Research Foundation | Photon-sorting spectroscopic microscope system |
| DE10222779A1 (de) * | 2002-05-16 | 2004-03-04 | Carl Zeiss Jena Gmbh | Verfahren und Anordnung zur Untersuchung von Proben |
| DE10222359B4 (de) * | 2002-05-21 | 2005-01-05 | Max Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Verfahren zur spektral differenzierenden, bildgebenden Messung von Fluoreszenzlicht |
| CA2920492C (en) * | 2013-09-30 | 2019-08-13 | Ventana Medical Systems, Inc. | Systems and methods for adaptive histopathology image unmixing |
| DE102018124129A1 (de) | 2017-12-04 | 2019-06-06 | Leica Microsystems Cms Gmbh | Mikroskopsystem und Verfahren zur mikroskopischen Abbildung mit einem solchen Mikroskopsystem |
| US11703454B2 (en) * | 2020-01-03 | 2023-07-18 | Korea Advanced Institute Of Science And Technology | Method and apparatus for multiplexed imaging of spectrally-similar fluorophores |
| DE102020107762A1 (de) | 2020-03-20 | 2021-09-23 | Leica Microsystems Cms Gmbh | Fluoreszenzmikroskop und Verfahren zur Abbildung eines Objekts |
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2021
- 2021-12-06 EP EP21212550.4A patent/EP4191234B1/en active Active
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2022
- 2022-11-28 US US17/994,434 patent/US12339229B2/en active Active
- 2022-12-05 JP JP2022194013A patent/JP2023084119A/ja active Pending
- 2022-12-05 CN CN202211581542.3A patent/CN116223457A/zh active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116879250A (zh) * | 2023-07-07 | 2023-10-13 | 宁波力显智能科技有限公司 | 荧光显微成像方法、装置、计算机设备和存储介质 |
Also Published As
| Publication number | Publication date |
|---|---|
| US12339229B2 (en) | 2025-06-24 |
| EP4191234B1 (en) | 2025-02-19 |
| EP4191234A1 (en) | 2023-06-07 |
| US20230175963A1 (en) | 2023-06-08 |
| JP2023084119A (ja) | 2023-06-16 |
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