CN116218725B - 一株热带醋杆菌及其在抗氧化、减脂以及抗衰老中的应用 - Google Patents
一株热带醋杆菌及其在抗氧化、减脂以及抗衰老中的应用 Download PDFInfo
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- CN116218725B CN116218725B CN202310076821.2A CN202310076821A CN116218725B CN 116218725 B CN116218725 B CN 116218725B CN 202310076821 A CN202310076821 A CN 202310076821A CN 116218725 B CN116218725 B CN 116218725B
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Abstract
本发明公开了一株热带醋杆菌及其在抗氧化、减脂以及抗衰老中的应用。该热带醋杆菌命名为热带醋杆菌(Acetobacter tropicalis)WY202203,其保藏编号为:GDMCC No:62704。本发明研究发现,该菌株WY202203能增强秀丽隐杆线虫的抗氧化能力,减少机体氧化损伤,延长线虫寿命,增加线虫体长,提高线虫行动力以及繁殖能力,减少脂肪积累及线粒体损伤。说明可将热带醋杆菌WY202203用于制备或开发抗氧化、减脂、抗衰老的产品中。
Description
技术领域
本发明属于益生菌技术领域,具体涉及一株热带醋杆菌及其在抗氧化、减脂以及抗衰老中的应用。
背景技术
日益加深的老龄化,正在给全球的经济、医疗等多方面带来越来越显著的影响。未富先老,也成为中国老龄化的基本特征。健康老龄化是指维护老年人的内在能力,改善老年人的外部环境,延长老年人的健康预期寿命;是应对人口老龄化成本最低、效益最好的手段和途径,因此,如何健康衰老已成为全球研究的热点。研究证实:益生菌如植物乳杆菌(Lactobacillus plantarum)、婴儿双歧杆菌(Bifidobacterium)、杜氏杆菌(Dubosiellanewyorkensis)、鼠李糖乳杆菌(Lactobacillus rhamnosus)、短乳杆菌(Lactobacillusbrevis)、乳酸片球菌(Pediococcus lactis)、戊糖片球菌(Pediococcus pentosus)等具有抗氧化、减脂作用,从而可有效延长健康寿命、延缓机体衰老,但这种作用效果具有明显的菌株特异性,因此继续挖掘具有抗氧化、减脂、抗衰老等功效的益生菌菌株具有广泛和实际的应用价值。
热带醋杆菌(Acetobacter tropicalis)主要用于食醋和果醋饮料生产的一种革兰氏阴性细菌。研究证实,热带醋杆菌能显著提高鱼、虾等水产动物的生长性能和抗病能力,有效改善因有机质过多导致的水体浑浊、发暗、发黑等现象,增加了溶氧,但热带醋杆菌的其它生理功效如抗氧化、减脂、抗衰老等鲜有报道。
发明内容
本发明要解决的技术问题是克服上述问题的缺陷和不足,提供一株热带醋杆菌及其在抗氧化、减脂、抗衰老中的应用。
本发明的第一个目的在于提供一株热带醋杆菌(Acetobacter tropicalis)WY202203,其保藏编号为:GDMCC No:62704。
本发明的第二个目的是提供一种微生物制剂,其包含上述的热带醋杆菌(Acetobacter tropicalis)WY202203。
优选的,所述的微生物制剂包含上述的热带醋杆菌(Acetobacter tropicalis)WY202203的菌体和/或其菌液作为活性成分。
优选的,所述的微生物制剂还包含能延长菌株活性时间的辅料,或其它微生物制剂可接受的辅料。
本发明提供的热带醋杆菌(Acetobacter tropicalis)WY202203菌株,已于2022年08月13日保藏于广东省微生物菌种保藏中心,保藏编号为GDMCC No:62704。所述WY202203菌株是来源于我国开菲尔(Kefir;一种传统酒精发酵乳饮料)中分离获得的,其16S rDNA基因序列长度为1354bp,测序后在NCBI使用Blast比对,鉴定为热带醋杆菌(Acetobactertropicalis)。菌株在MRS培养基上培养,是一种革兰氏阴性细菌,杆状、单个、成对或成链状;菌落为圆形、凸起、有光泽、不产色素。本发明研究表明,WY202203菌株喂食秀丽隐杆线虫后能延长线虫寿命、增加体长、提高产卵量以及行动力,增强线虫抗氧化能力,减少氧化损伤及脂肪积累,延缓机体衰老。
本发明使用的衰老研究模型动物秀丽隐杆线虫(Caenorhabditis elegans)N2,属于寿命研究模式动物,是一种简单的无脊椎动物模型,具有寿命短、生存环境简单、可大量同期化、遗传背景清晰、许多基因与哺乳动物(包括人类)在进化上保守性/同源性高等优点,可以从行为、分子和基因层次来研究衰老机制,且目前衰老机制的几大理论都是在线虫中被证实,因此,秀丽隐杆线虫是寿命、衰老研究常见的模式生物,对线虫有延长寿命、抗衰老作用的产品通常也被认为对人类也具有同样功效。
本发明的第三个目的是提供上述的热带醋杆菌(Acetobacter tropicalis)WY202203或微生物制剂在制备抗氧化、减脂和/或抗衰老产品中的应用。
本发明的第四个目的是提供一种抗氧化、减脂和/或抗衰老产品,其包含上述的热带醋杆菌(Acetobacter tropicalis)WY202203菌株和/或其菌液。
优选的,所述的菌液的浓度为2.6×107~8.6×1011CFU/mL。更优选的,所述的菌液的浓度为2.6×109CFU/mL。
优选的,所述的产品为保健食品、药品、饲料或饲料添加剂。
优选的,所述的产品的形态为液体、果冻、棒状、粉末型、片剂型、颗粒型或胶囊型。
优选地,所述的产品可针对不同年龄段动物;更优选的在于:从L1期开始使用。
优选地,所述动物泛指生物学分类上归属于动物界的生物,包括但不限于哺乳动物。
进一步优选地,所述动物为线虫。
本发明具有以下有益效果:
本发明提供了一株热带醋杆菌(Acetobacter tropicalis)WY202203菌株及其在抗氧化、减少脂肪积累及线粒体损伤、抗衰老且延长寿命中的应用。该菌株能增强秀丽隐杆线虫的抗氧化能力,减少机体氧化损伤,延长线虫寿命,增加线虫体长,提高线虫行动力以及繁殖能力,减少脂肪积累及线粒体损伤。说明可将热带醋杆菌WY202203用于制备或开发抗氧化、减脂、抗衰老的产品中。
热带醋杆菌(Acetobacter tropicalis)WY202203,其于2022年08月13日保藏于广东省微生物菌种保藏中心(GDMCC),地址:广东省广州市先烈中路100号大院59号楼5楼,邮编:510070,保藏编号为:GDMCC No:62704。
附图说明
图1为热带醋杆菌WY202203进化树。
图2为热带醋杆菌WY202203活菌、死菌及其代谢物对秀丽隐杆线虫N2寿命的影响。其中,OP50:饲喂大肠杆菌OP50;WY202203:饲喂热带醋杆菌WY202203活菌;WY202203supernatant:饲喂热带醋杆菌WY202203代谢物;WY202203heat-treat:饲喂热带醋杆菌WY202203死菌。
图3为热带醋杆菌WY202203饲喂秀丽隐杆线虫后的体长(A)和行动力(B)以及繁殖力(C)变化情况。其中,OP50:饲喂大肠杆菌OP50;WY202203:饲喂热带醋杆菌WY202203活菌。
图4为饲喂热带醋杆菌WY202203对线虫脂肪含量(A)和线粒体膜电位(B)的影响。其中,OP50:饲喂大肠杆菌OP50;WY202203:饲喂热带醋杆菌WY202203活菌。
图5为热带醋杆菌WY202203影响线虫ROS产量及抗氧化特性(A:MDA含量;B:ROS含量;C:SOD活性;D:GSH含量;E:CAT活性)。其中,OP50:饲喂大肠杆菌OP50;WY202203:饲喂热带醋杆菌WY202203活菌。
具体实施方式
以下结合说明书附图和具体实施例来进一步说明本发明,但实施例并不对本发明做任何形式的限定。除非特别说明,本发明采用的试剂、方法和设备为本技术领域常规试剂、方法和设备。
除非特别说明,以下实施例所用试剂和材料均为市购。
以下实施例中采用的试剂或配方:
NGM培养基:3g氯化钠,2.5g胰蛋白胨,17g琼脂粉,25mL磷酸钾缓冲液(1mol/L)、1mL CaCl2溶液(1mol/L)、1mL MgSO4溶液(1mol/L),加蒸馏水定容至1000mL,121℃高压灭菌20min。待温度降到60℃左右无菌条件下加入l mL胆固醇溶液(5mg/mL,95%乙醇溶解),摇匀后,倒平板。
LB液体培养基:5g酵母粉,10g胰蛋白胨,10g NaCl,加蒸馏水定容到1000mL,121℃高压灭菌20min。
MRS液体培养基:5g酪蛋白胨,10g牛肉膏,5g酵母膏,2g柠檬酸氢二胺,20g葡萄糖,5g乙酸钠,2g磷酸二氢钾,0.58g硫酸镁,0.25g硫酸锰,然后再加1mL吐温-80,蒸馏水定容至1000mL,121℃高压灭菌20min。
MRS固体培养基是在MRS液体培养基的配方基础上添加20g琼脂粉,制备得到。
野生型秀丽隐杆线虫N2、大肠杆菌OP50:购自于线虫中心CGC(CaenorhabditisGenetics Center)。
测量丙二醛(MDA)含量、超氧化物歧化酶(SOD)酶活性、活性氧(ROS)含量、谷胱甘肽(GSH)含量及过氧化氢酶(CAT)酶活性的试剂盒来源于上海碧云天生物技术有限公司。
实施例1:菌株的分离及鉴定
1、菌株的分离
采用9mL无菌生理盐水稀释1mL开菲尔(Kefir)发酵液,进行梯度稀释。将稀释液在MRS固体培养基上涂布,37℃培养,分离、纯化平板菌落,并将其在MRS液体培养基中培养进行初步筛选,得到一株菌株命名为WY202203。
2、传统的生物学鉴定
菌落形态特征:菌株WY202203在MRS培养基上培养,是一种革兰氏阴性细菌,杆状、单个、成对或成链状;菌落为圆形、凸起、有光泽、不产色素。
3、分子生物学鉴定
(1)采用DNA提取试剂盒提取菌株的总DNA,采用细菌的16SrDNA通用引物27F(5’-AGAGTTTGATCCTGGCTCAG-3’)、1492R(5’-TACGGCTACCTTGTTACGACTT-3’)经PCR扩增其16SrDNA基因。
PCR扩增程序为:98℃预变性2min,98℃变性10s,55℃退火10s,72℃延伸25s,72℃终延伸2min,循环30次。
PCR扩增体系为:27F 1μL,1492R 1μL,DNA模板2μL,Taq酶22μL。
(2)系统发育分析
PCR产物经1%琼脂糖凝胶检测并切胶回收纯化后测序分析,将长度为1354bp的16SrDNA基因序列(如SEQ ID NO.1所示)与Genbank中已收录的基因序列进行比对及进化树比对分析,结果如图1所示,发现WY202203菌株与热带醋杆菌(Acetobacter tropicalis)最相似,相似度为99.85%。
菌株WY202203的16SrDNA基因序列如SEQ ID NO.1所示,具体为:CGGCTGCGCCCCTTGCGGGTTCGCTCACCGGCTTAAGGTCAAACCAACTCCCATGGTGTGACGGGCG GTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCACCTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGACGGCTTTTAGAGATCAGCACGATGTCGCCATCTAGCTTCCCACTGTCACCGCCATTGTAGCACGTGTGTAGCCCAGGACATAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGGCTTGTCACCGGCAGTCTCTCTAGAGTGCCCACCCAAACATGCTGGCAACTAAAGATAGGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGCTGTAGGTCCCTTGCGGGAAATGCCCATCTCTGGACACAGCCTACACATACAAGCCCTGGTAAGGTTCTGCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCAACCTTGCGGCCGTACTCCCCAGGCGGTGTGCTTATCGCGTTAGCTACGACACTGAACAACTAAGTTGCCCAACATCCAGCACACATCGTTTACAGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTCATGAGCCAGGTTGCCGCCTTCGCCACCGGTGTTCTTCCCAATATCTACGAATTTCACCTCTACACTGGGAATTCCACAACCCTCTCTCACACTCTAGTCTGCACGTATCAAATGCAGCTCCCAGGTTAAGCCCGGGGATTTCACATCTGACTGTACAAACCGCCTACACGCCCTTTACGCCCAGTCATTCCGAGCAACGCTAGCCCCCTTCGTATTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTGCGGGTACCGTCATCATCGTCCCCGCCGAAAGTGCTTTACAATCCGAAAACCTTCTTCACACACGCGGCATTGCTGGATCAGGGTTGCCCCCATTGTCCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCTGATCATCCTCTCAGACCAGCTATCGATCATCGCCTTGGTAGGCCATTACCCCACCAACAAGCTAATCGAACGCAGGCTCCTCCACAGGCGACTTGCGCCTTTGACCCTCAGGTATCATGCGGTATTAGCTCCAGTTTCCCAGAGTTATCCCCCACCCATGGATAGATTCCTACGCGTTACTCACCCGTCCGCCACTAAGGCCGAAACCTTCGTGCGACTG。
综合上述的形态特性和16SrDNA基因序列结果,表明本发明所筛选的细菌应归属于醋酸杆菌属(Acetobacter)的热带醋杆菌(Acetobacter tropicalis),命名为热带醋杆菌(Acetobacter tropicalis)WY202203菌株,该菌株于2022年08月13日保藏于广东省微生物菌种保藏中心(GDMCC),保藏编号为:GDMCC No:62704,地址:广东省广州市先烈中路100号大院59号楼5楼,邮编:510070。
实施例2:热带醋杆菌WY202203对秀丽隐杆线虫寿命试验
1、菌种的活化
取冻存于-80℃下的热带醋杆菌WY202203 100μL,接种于5mL MRS液体培养基中,放入37℃生化培养箱中静置培养20~24h,使菌株活化。
2、秀丽隐杆线虫的培养
在NGM培养基上涂布大肠杆菌OP50作为线虫食物,然后挑取野生型秀丽隐杆线虫N2置于NGM培养基表面,并放置于20℃生化培养箱里培养,培养期间需每隔2天将线虫转移到新鲜的涂有大肠杆菌OP50的NGM平板上。
3、秀丽隐杆线虫的同期化
将L4期线虫收集到离心管里,加入1mL M9缓冲液洗去多余的大肠杆菌OP50,静置沉淀后去除上清液,重复洗3次。然后加入1mL裂解液充分振荡,去除上清液后,再用M9缓冲液进行离心(3000r/min,1min)洗涤3次,去除上清液并保留虫卵,将虫卵转移至新的NGM平板上,于20℃生化培养箱孵化,大约16h后生长成L1期幼虫。
4、秀丽隐杆线虫寿命的测定
将热带醋杆菌WY202203和大肠杆菌OP50分别在MRS和LB液体培养基培育至对数期,在4℃,8000r/min离心10min,收集热带醋杆菌WY202203菌体及发酵上清液,经过0.22μm滤膜过滤的发酵上清液作为待测菌株的代谢物。同时,将待测热带醋杆菌WY202203菌体经过100℃、15min灭活,制备得到待测死菌体;分别以热带醋杆菌WY202203代谢物与大肠杆菌OP50菌液按1:1比例复配的混合物(2.1×109CFU/mL),热带醋杆菌WY202203死菌体与无菌水重悬的混合物(1.5×109CFU/mL),以及热带醋杆菌WY202203的活菌(2.6×109CFU/mL)作为食物,以只喂养大肠杆菌OP50(1.3×108CFU/mL)作为对照,分别喂养秀丽隐杆线虫N2并测定寿命。
挑取90条上述步骤同期化的L1期秀丽隐杆线虫N2,分别放置到含有热带醋杆菌WY202203活菌、死菌及其代谢物以及大肠杆菌OP50的NGM平板上,并置于20℃生化培养箱培养。每隔1天挑取全部存活的线虫转移到新鲜的涂布菌液的NGM平板上,防止有新孵化的线虫影响实验结果。实验持续到线虫全部死亡,并绘制线虫寿命曲线。
5、试验结果
结果如图2所示,与喂食大肠杆菌OP50平均寿命(10d)及最长寿命(20d)相比热带醋杆菌WY202203的活菌、死菌及其代谢物的平均寿命分别显著延长42.5%、14.1%、24.7%(p<0.05),最长寿命分别延长7天、2天和4天,上述结果说明热带醋杆菌WY202203的活菌、死菌及其代谢物能显著延长线虫寿命。
实施例3:热带醋杆菌WY202203对秀丽隐杆线虫体长、运动力及繁殖力的影响
1、试验方法
(1)为验证热带醋杆菌WY202203是否影响线虫的身体长度,挑取同期化培养到第3天的线虫,用70℃热水浴浸泡30min,使线虫身体僵直并吸取虫体于载玻片上,用光学显微镜观察并测量线虫的体长,每组实验至少重复10条线虫。
(2)运动能力是健康的关键指标之一,运动状况和运动能力会随着机体的衰老而逐渐变差和减弱。采用热带醋杆菌WY202203菌株培养秀丽隐杆线虫,培养方法同实施例2,以只喂养大肠杆菌OP50作为对照组;在第3天随机挑取20条线虫到新鲜的NGM平板上,线虫自由活动30s后用体视显微镜观察1min内线虫头部摆动次数,一次头部摆动则为线虫头部从一侧摆动另一侧为一次。
(3)筛选的抗衰老药物或菌株应以不损害机体生殖能力为前提条件,生殖能力是健康中最为关键的指标之一。因此以后代数量为指标验证了热带醋杆菌WY202203对线虫繁殖能力的影响。按实施例2所述方法用待测菌株培养秀丽隐杆线虫直至L4期,随机挑取1条线虫分别到涂有热带醋杆菌WY202203、大肠杆菌OP50的NGM平板上,每组5个平行。在线虫产卵期间,每隔1d将产卵的成虫转移到新的平板上,旧平板则继续放置20℃生化培养箱培养至虫卵孵化,统计平板上线虫总孵化的幼虫条数,直到线虫产卵结束。
2、实验结果
与大肠杆菌OP50对照组相比,热带醋杆菌WY202203组的线虫第3天体长延长了26.2%,(图3A,p<0.05),头部摆动频率增加了25.8%(图3B,p<0.05),总产卵量增加了36.9%(图3C,p<0.05)。说明饲喂热带醋杆菌WY202203能显著增加线虫体长、增强线虫的行动能力与繁殖能力,具有较好的抗衰老性能。
实施例4:热带醋杆菌WY202203对线虫脂肪含量、线粒体膜电位的影响
1、试验方法
(1)热带醋杆菌WY202203对线虫体内脂肪含量的影响
采用热带醋杆菌WY202203培养秀丽隐杆线虫,培养方法同实施例2,以喂养大肠杆菌OP50作为对照组,在L4期继续培养3天后随机挑取20条线虫用红油O染色法测量线虫脂肪。用M9缓冲液冲洗3次,25mM盐酸左旋咪唑麻醉静止线虫,加入200μL 4%多聚甲醛溶液静置15~20min,后移除溶液再用液氮冻融3次,然后用60%异丙醇红油O染色,4~5h后用M9缓冲液冲洗多余染料,并用光学显微镜观察。
(2)热带醋杆菌WY202203对秀丽隐杆线虫体内线粒体膜电位的影响
采用热带醋杆菌WY202203培养秀丽隐杆线虫,培养方法同实施例2,以喂养大肠杆菌OP50作为对照组,在L4期继续培养3天后随机挑取20条线虫用JC-1染料测定线粒体膜电位,将线虫与现配的JC-1染液避光孵育2小时,M9缓冲液冲洗去线虫表面的染料,通过荧光显微镜观察红色荧光(发射波长约590nm)和绿色荧光(发射波长529nm),拍照记录,并用Image J软件对荧光照片进行定量计算。
2、试验结果
与大肠杆菌OP50对照组相比,热带醋杆菌WY202203喂养的线虫肠道脂肪红光光密度减少43.7%(图4A,p<0.05),JC-1染色后的红绿荧光比值增加47.6%(图4B,p<0.05)。说明热带醋杆菌WY202203显著降低线虫体内脂肪沉积;减缓线粒体膜电位下降程度、减少线粒体氧化受损。
实施例5:热带醋杆菌WY202203对秀丽隐杆线虫的抗氧化能力的影响
1、试验方法
(1)采用热带醋杆菌WY202203菌株培养秀丽隐杆线虫,培养方法同实施例2,以只喂养大肠杆菌OP50作为对照组,L4期继续培养3天后收集线虫,M9缓冲液清洗3次后,待线虫自然沉降。将线虫放入匀浆器,冰上研磨,4℃、4000r/min离心取上清液,即为蛋白匀浆。根据试剂盒操作说明测量丙二醛(MDA)含量、活性氧(ROS)水平、超氧化物歧化酶(SOD)酶活性、谷胱甘肽(GSH)含量及过氧化氢酶(CAT)酶活性。
(2)丙二醛含量测定:根据试剂盒说明配置好TBA储存液(避光储存)、MDA检测工作液,空白组(0.1mL PBS+0.2mLMDA检测工作液)、样品组(0.1mL样品+0.2mL MDA检测工作液)沸水浴加热15min,待冷却至室温后取上清液200μL加入到96孔板中,随后用酶标仪在532nm测定吸光度。
(3)活性氧水平测定:将50μL蛋白匀浆和50μL现配的100mmol/L H2-DCF-DA溶液加入96孔黑板中(最终浓度为50mmol/L)处理3h,荧光酶标仪于激发波长485nm、发射波长535nm下测定体内活性氧含量。
(4)超氧化物歧化酶活性测定:配置好SOD检测缓冲液、WST-8/酶工作液和反应启动液,按照试剂盒加样量分别配置样品组和空白组,37℃孵育30min后在450nm出测吸光度。
(5)谷胱甘肽含量测定:根据试剂盒分别配置好GSH储备液、DTNB储备液和NADPH储备液之后,进行对照组和样品组的加样,立即用酶标仪在412nm处测定吸光度。
(6)过氧化氢酶活性测定:根据试剂盒说明配置好过氧化氢酶检测缓冲液、250mM过氧化氢溶液、过氧化氢酶反应终止液,进行对照组和样品组的加样,25℃至少孵育15分钟后在520nm处测定吸光度。
2、试验结果
与大肠杆菌OP50的对照组相比,热带醋杆菌WY202203喂养的线虫体内MDA含量显著减少37.3%(图5A,p<0.05),ROS水平下降了19.3%(图5B,p<0.05),SOD活性显著提高了36.4%(图5C,p<0.05),GSH含量显著提高了35.8%(图5D,p<0.05),CAT活性显著提高了69.6%(图5E,p<0.05),以上结果表明热带醋杆菌WY202203可通过增加SOD酶活、CAT酶活和GSH含量,减少体内MDA含量和ROS水平,来提升线虫机体的抗氧化能力。
综上可知,本发明提供的热带醋杆菌(Acetobacter tropicalis)WY202203菌株能延长线虫寿命及体长、增强行动力及繁殖能力,减少脂肪积累,减少线粒体氧化受损,提高SOD、GSH、CAT等产量且减少体内氧自由基合成,具有抗氧化、减脂、抗衰老的功效。
以上仅是本发明的优选实施方式,需要说明的是,上述实施方式不以任何形式限制本发明,凡采用等同替换或等效变换的方式所获得的技术方案,均落在本发明的保护范围内。
Claims (10)
1.一株热带醋杆菌(Acetobacter tropicalis)WY202203,其保藏编号为:GDMCC No:62704。
2.一种微生物制剂,其特征在于,包含权利要求1所述的热带醋杆菌(Acetobactertropicalis)WY202203。
3.根据权利要求2所述的微生物制剂,其特征在于,所述的微生物制剂包含所述的热带醋杆菌(Acetobacter tropicalis)WY202203的菌体和/或其菌液作为活性成分。
4.根据权利要求2或3所述的微生物制剂,其特征在于,所述的微生物制剂还包含能延长菌株活性时间的辅料,或其它微生物制剂可接受的辅料。
5.权利要求1所述的热带醋杆菌(Acetobacter tropicalis)WY202203或权利要求2所述的微生物制剂在制备抗氧化、减脂和/或抗衰老产品中的应用。
6.一种抗氧化、减脂和/或抗衰老产品,其特征在于,包含权利要求1所述的热带醋杆菌(Acetobacter tropicalis)WY202203菌株和/或其菌液。
7.根据权利要求6所述的抗氧化、减脂和/或抗衰老产品,其特征在于,所述的菌液的浓度为2.6×107~8.6×1011CFU/mL。
8.根据权利要求7所述的抗氧化、减脂和/或抗衰老产品,其特征在于,所述的菌液的浓度为2.6×109CFU/mL。
9.根据权利要求6-8任一项所述的抗氧化、减脂和/或抗衰老产品,其特征在于,所述的产品为保健食品、药品、饲料或饲料添加剂。
10.根据权利要求6-8任一项所述的抗氧化、减脂和/或抗衰老产品,其特征在于,所述的产品的形态为液体、果冻、棒状、粉末型、片剂型、颗粒型或胶囊型。
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