CN116199334B - Method for strengthening biological denitrification efficiency of low-temperature activated sludge by chlorophyll - Google Patents
Method for strengthening biological denitrification efficiency of low-temperature activated sludge by chlorophyll Download PDFInfo
- Publication number
- CN116199334B CN116199334B CN202310325514.3A CN202310325514A CN116199334B CN 116199334 B CN116199334 B CN 116199334B CN 202310325514 A CN202310325514 A CN 202310325514A CN 116199334 B CN116199334 B CN 116199334B
- Authority
- CN
- China
- Prior art keywords
- activated sludge
- low
- temperature
- nitrogen
- chlorophyll
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000010802 sludge Substances 0.000 title claims abstract description 78
- 229930002875 chlorophyll Natural products 0.000 title claims abstract description 37
- 235000019804 chlorophyll Nutrition 0.000 title claims abstract description 37
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000005728 strengthening Methods 0.000 title claims abstract description 12
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 84
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 42
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 claims abstract description 27
- 239000010865 sewage Substances 0.000 claims abstract description 14
- 230000000694 effects Effects 0.000 claims abstract description 10
- 230000027756 respiratory electron transport chain Effects 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 26
- 239000002351 wastewater Substances 0.000 claims description 15
- 229910002651 NO3 Inorganic materials 0.000 claims description 13
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims description 13
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 claims description 9
- 235000013619 trace mineral Nutrition 0.000 claims description 9
- 239000011573 trace mineral Substances 0.000 claims description 9
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 claims description 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 6
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 6
- 229910052799 carbon Inorganic materials 0.000 claims description 6
- 230000015556 catabolic process Effects 0.000 claims description 6
- 238000006731 degradation reaction Methods 0.000 claims description 6
- 229910052698 phosphorus Inorganic materials 0.000 claims description 6
- 239000011574 phosphorus Substances 0.000 claims description 6
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 4
- 238000005273 aeration Methods 0.000 claims description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 4
- 229910052760 oxygen Inorganic materials 0.000 claims description 4
- 239000001301 oxygen Substances 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 230000002708 enhancing effect Effects 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 230000001737 promoting effect Effects 0.000 claims description 2
- 238000007789 sealing Methods 0.000 claims description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 abstract description 2
- 235000017281 sodium acetate Nutrition 0.000 abstract description 2
- 239000001632 sodium acetate Substances 0.000 abstract description 2
- -1 biochar Chemical class 0.000 description 7
- 230000008859 change Effects 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 230000009467 reduction Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 3
- 239000011734 sodium Substances 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000001546 nitrifying effect Effects 0.000 description 2
- 230000033116 oxidation-reduction process Effects 0.000 description 2
- 238000006479 redox reaction Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 108091006149 Electron carriers Proteins 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001491 aromatic compounds Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000004134 energy conservation Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000003864 humus Chemical class 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000001699 photocatalysis Effects 0.000 description 1
- 238000007146 photocatalysis Methods 0.000 description 1
- 150000004053 quinones Chemical class 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000005067 remediation Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/163—Nitrates
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/166—Nitrites
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/38—Organic compounds containing nitrogen
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
A method for strengthening biological denitrification efficiency of low-temperature activated sludge by chlorophyll belongs to the technical field of biological sewage treatment, and the method utilizes the action of chlorophyll electron transfer of a natural redox mediator to accelerate the transfer of electrons of an electron donor (sodium acetate) to an electron acceptor (nitrate nitrogen), thereby effectively improving the denitrification rate and the total nitrogen removal rate of a low-temperature activated sludge system. The method comprises three steps: 1) Determining the dosage of the redox mediator; 2) Basic domestication of low-temperature activated sludge; 3) Enhanced acclimation of the low temperature redox mediator. Through the electron transfer effect of chlorophyll, the denitrification rate of the activated sludge system at low temperature is improved by 1.37-3.94 times, and the total nitrogen removal rate is improved by 4.47-60.71%. The redox mediator chlorophyll adopted by the invention is naturally occurring, and can provide a certain technical guide for solving the problems of low denitrification efficiency and high operation cost in winter of the existing activated sludge system.
Description
Technical Field
The invention belongs to the technical field of biological sewage treatment, and particularly relates to a method for strengthening biological denitrification efficiency of low-temperature activated sludge by using chlorophyll as a natural redox mediator. Is suitable for the fields of municipal domestic sewage treatment and the like under the low-temperature condition in winter in the north.
Background
Nitrogen is an important index for eutrophication of water. In addition, nitrate nitrogen can have serious effects on various organisms, particularly aquatic products and human health. The activated sludge process is a main flow process of biological denitrification of urban sewage treatment plants, and mainly comprises two stages of nitrification and denitrification, wherein microorganisms for completing the two stages of nitrification and denitrification are nitrifying bacteria and denitrifying bacteria respectively. In northeast, northwest and North China, due to high latitude, long winter time, low sewage temperature and sustainable time of day average temperature below 15 ℃ for nearly half a year. A great deal of research shows that the low temperature has obvious influence on the sedimentation performance, adsorption performance, microbial growth and development in the activated sludge, population composition and metabolic activity, and finally, the starting and running of the urban sewage treatment plant are difficult. The research shows that in the sewage treatment process of the activated sludge system, the water temperature for ensuring the normal growth and metabolism of microorganisms is 20-35 ℃, and denitrifying bacteria seem to be more susceptible to the temperature than nitrifying bacteria along with the reduction of the temperature, and the denitrification effect of the denitrifying bacteria can be serious when the water temperature is about 10 ℃. At present, the sewage treatment plant in northern China with low air temperature usually adopts measures such as artificially diluting the wastewater, prolonging the residence time of the wastewater, reducing the sludge load, raising the temperature and the like to ensure that the wastewater reaches the standard. These measures not only greatly increase the running cost of sewage treatment plants, but also make the treatment effect unstable and even sometimes cause sludge bulking. Therefore, the method solves the problem of low denitrification efficiency of the low-temperature activated sludge system and has important significance for energy conservation and consumption reduction of sewage treatment plants in winter and standard emission.
Redox Mediators (RMs), also known as electron shuttles, are an organic molecule that can undergo reversible oxidation and reduction, have the ability to act as electron carriers in a variety of redox reactions, and can increase the redox reaction rate by one to several orders of magnitude. The addition of RMs can improve the denitrification rate and the denitrification efficiency of biological denitrification and improve the carbon source utilization rate of an activated sludge system. RMs which has been reported at present and can catalyze biological denitrification comprises quinone compounds, humus compounds, biochar, porphyrin compounds, endogenous compounds and the like, and the porphyrin compounds are intensively studied due to excellent electron transfer characteristics. Porphyrin compound is aromatic compound with 18 kinds of pi electrons and attractive electron emission/absorption property, and is widely applied to the fields of photocatalysis, oxidation reduction, catalysis and the like. However, the porphyrin compound has complex synthesis, harsh reaction conditions, low yield, difficult separation of products and other problems, and limits the application of the porphyrin compound in environmental remediation. Chlorophyll is a natural porphyrin compound, widely exists in plants, and has the advantages of simple extraction, low cost, environmental friendliness and the like. The forward biocatalysis of chlorophyll to denitrification is reported, and chlorophyll can improve the reduction rate of nitrate and nitrite and has good recycling property. The strengthening effect of chlorophyll is rarely reported for low-temperature complex activated sludge systems. The invention provides a method for strengthening denitrification efficiency of a low-temperature activated sludge system by using natural redox mediator chlorophyll, which provides a certain technical guide for solving the problems of low-temperature denitrification efficiency and high operation cost in winter of the existing activated sludge system.
Disclosure of Invention
The invention provides a method for strengthening biological denitrification efficiency of low-temperature activated sludge by using chlorophyll as a natural redox mediator, which aims to solve the problems of low biological denitrification efficiency and high operation cost of low-temperature activated sludge in winter.
The technical scheme provided by the invention is that the method for strengthening biological denitrification efficiency of low-temperature activated sludge by using natural redox mediator chlorophyll, which utilizes the effect of electron transfer of natural redox mediator chlorophyll to accelerate the transfer of electrons of an electron donor (sodium acetate) to an electron acceptor (nitrate nitrogen) and effectively improves the denitrification rate and the total nitrogen removal rate of a low-temperature activated sludge system, comprises the following steps:
1) Determination of the dose of redox mediator: determining the chlorophyll adding amount of the redox mediator to be 0.02mmol/L-0.10mmol/L;
2) Basic domestication of low-temperature activated sludge: activated sludge is collected in activated sludge in an aeration tank of a municipal sewage treatment plant, an intermittent operation mode is adopted, activated sludge and nitrate wastewater are added into a reactor, an anoxic condition is achieved through sealing, the reactor is placed in a constant-temperature vibrating box for reaction, wherein the volume of the activated sludge and the nitrate wastewater is 1:1, the sludge concentration is 4g/L, the water discharge ratio is 50%, the low-temperature condition is 13-15 ℃, the dissolved oxygen DO is less than or equal to 0.2mg/L, and the rotating speed of the constant-temperature vibrating box is 110-120 rad/min; the reactor is operated for 3 periods, the nitrate nitrogen, nitrite nitrogen and total nitrogen concentration in the water entering and exiting the reactor are measured every period, and when the degradation of the nitrate nitrogen and the total nitrogen of the activated sludge system is stable, the sludge system is stable, namely the low-temperature domestication is completed;
3) Enhanced acclimatization of low temperature redox mediators: adding chlorophyll redox mediators into the activated sludge system subjected to basic domestication every period, and enhancing biological denitrification efficiency of the low-temperature activated sludge system by utilizing the effect of promoting electron transfer of the activated sludge system by using the redox mediators, wherein the concentration of the chlorophyll adding agents of the redox mediators in the system is 0.02mmol/L-0.10mmol/L, and the concentration of nitrate nitrogen, nitrite nitrogen and total nitrogen in water entering and exiting a reactor are measured every period, and after the degradation of the nitrate nitrogen and the total nitrogen of the activated sludge system is stable, calculating a period; basically, the first period of the invention achieves the effect of stability, namely the degradation of nitrate nitrogen and total nitrogen of the activated sludge system corresponding to the first period and the subsequent continuous 3 periods is stable and consistent, which indicates that the sludge system is fast in strengthening and stabilizing, namely the strengthening and domesticating speed is fast, and the stability is strong.
Meanwhile, in the step 3), the first period of the reactor is sampled and measured in a full period to determine the concentration of nitrate nitrogen and total nitrogen, and the biological denitrification efficiency of the activated sludge system is analyzed.
Nitrate wastewater adopted in the step 2) and the step 3) is configured manually by NaNO 3 Is a nitrogen source, CH 3 COONa is carbon source, KH 2 PO 4 Is a phosphorus source, and trace elements are 1ml/L; the nitrate nitrogen concentration is 60mg/L, the COD concentration is 480-600 mg/L, the carbon-nitrogen ratio of the inlet water is 8-10, and the composition and the concentration of the trace element solution are as follows: na (Na) 2 EDTA 4.29g/L, feCl 2 ·4H 2 O is 1.99g/L, mnCl 2 ·2H 2 O is 0.08g/L, niCl 2 ·6H 2 O is 0.02g/L, coCl 2 ·6H 2 O is 0.02g/L, cuCl 2 ·H 2 O is 0.02g/L, znCl 2 0.02g/L NaMoO 4 ·2H 2 O is 0.02g/L, na 2 WoO 4 ·2H 2 O is 0.03g/L, H 3 BO 3 0.06g/L.
Step 3), the operation conditions are as follows in step 2): the volume of the activated sludge and the nitrate wastewater is 1:1, the drainage ratio is 50%, the low temperature condition is 13-15 ℃, the dissolved oxygen DO is less than or equal to 0.2mg/L, and the rotating speed of the constant temperature vibrating box is 110-120 rad/min.
Efficacy analysis: the biological denitrification efficiency of the low-temperature activated sludge system is enhanced by adding chlorophyll redox mediators. After the reinforced domestication of chlorophyll oxidation-reduction mediators, the denitrification rate of the activated sludge system is increased from 0.58mg N/(h.g MLSS) to 0.80-3.95 mg N/(h.g MLSS), the denitrification rate is increased by 1.37-3.94 times, and the total nitrogen removal rate is increased from 11.72% to 16.19% -80.38%. And the denitrification efficiency of the activated sludge system is better along with the increase of the chlorophyll dosage.
The invention has the beneficial effects that the denitrification efficiency of the activated sludge system domesticated at low temperature is obviously improved by adding chlorophyll redox mediators with different dosages. Under the low temperature condition, the COD concentration of the inlet water is 480-600 mg/L, the nitrate nitrogen concentration is 60mg/L, and the adding of chlorophyll redox medium into the activated sludge system with the inlet water carbon nitrogen ratio of 8-10 can improve the denitrification rate of the activated sludge system by 1.37-3.94 times and the total nitrogen removal rate by 4.47-60.71 percent. The chlorophyll redox mediator strengthens the denitrification efficiency of the low-temperature activated sludge system, and provides a certain technical guide for effectively solving the problems of low denitrification efficiency and high operation cost of the low-temperature activated sludge in winter. Compared with other methods for improving the problem of low denitrification efficiency of a low-temperature activated sludge system in winter, the method has the advantages of simplicity in operation, easiness in implementation, convenience in management and the like.
Drawings
FIG. 1 is a graph showing the denitrification rate, nitrate nitrogen change, total nitrogen change and total nitrogen removal rate change in the first cycle at a feed water carbon nitrogen ratio of 8;
FIG. 2 shows the water inlet and outlet conditions and total nitrogen removal rate per cycle at a water inlet carbon nitrogen ratio of 8;
FIG. 3 is a graph showing the denitrification rate, nitrate nitrogen change, total nitrogen change and total nitrogen removal rate change for the first cycle at a feed carbon to nitrogen ratio of 10;
FIG. 4 shows the water inlet and outlet conditions and total nitrogen removal rate per cycle at a water inlet carbon nitrogen ratio of 10;
Detailed Description
The invention is further illustrated below in connection with specific examples, but the scope of the invention is not limited thereto.
Example 1
The redox mediator is chlorophyll, and the dosage of chlorophyll is 0.02mmol/L, 0.05mmol/L and 0.1mmol/L respectively; the activated sludge is sourced from an aeration tank of a municipal sewage treatment plant in Beijing city, and the sludge concentration is 4g/L.
Under the low temperature condition, 75ml of activated sludge and 75ml of nitrate wastewater are added into four conical flask reactors for low temperature activated sludge basic domestication, the basic domestication is respectively marked as No. 1, no. 2, no. 3 and No. 4, the conical flask specification is 250ml, and the basic domestication is operated for 3 periods. Wherein the nitrate wastewater is prepared manually by NaNO 3 Is a nitrogen source, CH 3 COONa is carbon source, KH 2 PO 4 Is a phosphorus source, and trace elements are 1ml/L. The nitrate nitrogen concentration of the inlet water is 60mg/L, the COD concentration is 480mg/L, the phosphorus concentration is 12mg/L, the carbon-nitrogen ratio of the inlet water is 8, and the composition and concentration of the trace element solution are as follows: na (Na) 2 EDTA 4.29g/L, feCl 2 ·4H 2 O is 1.99g/L, mnCl 2 ·2H 2 O is 0.08g/L, niCl 2 ·6H 2 O is 0.02g/L, coCl 2 ·6H 2 O is 0.02g/L, cuCl 2 ·H 2 O is 0.02g/L, znCl 2 0.02g/L NaMoO 4 ·2H 2 O is 0.02g/L, na 2 WoO 4 ·2H 2 O is 0.03g/L, H 3 BO 3 0.06g/L. And (3) performing intensive domestication of the redox mediator on the activated sludge system subjected to basic domestication, wherein the 1# is taken as a blank control group, chlorophyll redox mediator is added into the 2# reactor, the 3# reactor and the 4# reactor in each period, and the adding dosages are respectively 0.02mmol/L, 0.05mmol/L and 0.1mmol/L. The intensive domestication is operated for 3 cycles, the first cycle is used for sampling and measuring the concentration of nitrate nitrogen, nitrite nitrogen and total nitrogen in the whole cycle, and the other cycles are used for measuring the concentration of nitrate nitrogen, nitrite nitrogen and total nitrogen in the water.
After the treatment by the method, as shown in fig. 1, in the first period of the intensive domestication, the denitrification rates of the reactors with the dosage of 0.02mmol/L, 0.05mmol/L and 0.1mmol/L respectively reach 0.80, 1.72 and 2.28mg N/(h.g MLSS), the denitrification rate of the control group is improved by 1.37, 2.97 and 3.94 times compared with the denitrification rate of 0.58mg N/(h.g MLSS), the total nitrogen removal rate reaches 16.19%, 35.02% and 47.06%, and the total nitrogen removal rate of the control group is improved by 4.47%, 23.30% and 35.34% compared with the total nitrogen removal rate of 11.72%. Meanwhile, as shown in fig. 2, the total nitrogen removal rate is stable in 3 cycles of the activated sludge system subjected to chlorophyll strengthening domestication.
Example 2
The redox mediator is chlorophyll, and the dosage of chlorophyll is 0.02mmol/L, 0.05mmol/L and 0.1mmol/L respectively; the activated sludge is sourced from an aeration tank of a municipal sewage treatment plant in Beijing city, and the sludge concentration is 4g/L.
Under the low temperature condition, 75ml of activated sludge and 75ml of nitrate wastewater are added into four conical flask reactors for low temperature activated sludge basic domestication, the basic domestication is respectively marked as No. 5, no. 6, no. 7 and No. 8, the conical flask specification is 250ml, and the basic domestication is operated for 3 periods. Wherein the nitrate wastewater is prepared manually by NaNO 3 Is a nitrogen source, CH 3 COONa is carbon source, KH 2 PO 4 The trace element solution is a phosphorus source, the trace element is 1ml/L, the nitrate nitrogen concentration of the inflow water is 60mg/L, the COD concentration is 600mg/L, the phosphorus concentration is 12mg/L, the carbon nitrogen ratio of the inflow water is 10, and the trace element solution comprises the following components in percentage by weight: na (Na) 2 EDTA 4.29g/L, feCl 2 ·4H 2 O is 1.99g/L, mnCl 2 ·2H 2 O is 0.08g/L, niCl 2 ·6H 2 O is 0.02g/L, coCl 2 ·6H 2 O is 0.02g/L, cuCl 2 ·H 2 O is 0.02g/L, znCl 2 0.02g/L NaMoO 4 ·2H 2 O is 0.02g/L, na 2 WoO 4 ·2H 2 O is 0.03g/L, H 3 BO 3 0.06g/L. And (3) carrying out intensive domestication of the redox mediator on the activated sludge system subjected to basic domestication, taking the No. 5 as a blank control group, and adding chlorophyll redox mediator into the No. 6, no. 7 and No. 8 reactors at the dosages of 0.02mmol/L, 0.05mmol/L and 0.1mmol/L respectively. The intensive domestication is operated for 3 cycles, the first cycle is used for sampling and measuring the concentration of nitrate nitrogen, nitrite nitrogen and total nitrogen in the whole cycle, and the other cycles are used for measuring the concentration of nitrate nitrogen, nitrite nitrogen and total nitrogen in the water.
After the treatment by the method, as shown in fig. 1, in the first period of the intensive domestication, the denitrification rates of the reactors with the addition dosages of 0.02mmol/L, 0.05mmol/L and 0.1mmol/L respectively reach 1.60, 3.09 and 3.95mg N/(h.g MLSS), the denitrification rate of the control group is improved by 1.57, 3.04 and 3.89 times compared with the denitrification rate of 1.01mg N/(h.g MLSS), the total nitrogen removal rate reaches 31.43%, 60.66% and 80.38%, and the total nitrogen removal rate of the control group is improved by 11.76%, 40.99% and 60.71% compared with the total nitrogen removal rate of 19.67%. Meanwhile, as shown in fig. 4, the total nitrogen removal rate is stable in 3 cycles of the activated sludge system subjected to chlorophyll-enhanced acclimation.
The above embodiments are not intended to limit the present invention, and the present invention is not limited to the above embodiments, and falls within the scope of the present invention as long as the present invention meets the requirements.
Claims (4)
1. The method for enhancing biological denitrification efficiency of the low-temperature activated sludge by using chlorophyll is characterized in that the method accelerates the transfer of electrons of an electron donor to an electron acceptor by utilizing the action of chlorophyll electron transfer of a natural redox mediator, and effectively improves the denitrification rate and the total nitrogen removal rate of a low-temperature activated sludge system, and comprises the following operation steps:
1) Determination of the dose of redox mediator: determining the chlorophyll adding amount of the redox mediator to be 0.02mmol/L-0.10mmol/L;
2) Basic domestication of low-temperature activated sludge: activated sludge is collected in activated sludge in an aeration tank of a municipal sewage treatment plant, an intermittent operation mode is adopted, activated sludge and nitrate wastewater are added into a reactor, an anoxic condition is achieved through sealing, the reactor is placed in a constant-temperature vibrating box for reaction, wherein the volume of the activated sludge and the nitrate wastewater is 1:1, the sludge concentration is 4g/L, the water discharge ratio is 50%, the low-temperature condition is 13-15 ℃, the dissolved oxygen DO is less than or equal to 0.2mg/L, and the rotating speed of the constant-temperature vibrating box is 110-120 rad/min; the reactor is operated for 3 periods, the nitrate nitrogen, nitrite nitrogen and total nitrogen concentration in the water entering and exiting the reactor are measured every period, and when the degradation of the nitrate nitrogen and the total nitrogen of the activated sludge system is stable, the sludge system is stable, namely the low-temperature domestication is completed;
3) Enhanced acclimatization of low temperature redox mediators: adding chlorophyll redox mediators into the activated sludge system subjected to basic domestication every period, and enhancing biological denitrification efficiency of the low-temperature activated sludge system by utilizing the effect of promoting electron transfer of the activated sludge system by using the redox mediators, wherein the concentration of the chlorophyll adding agents of the redox mediators in the system is 0.02mmol/L-0.10mmol/L, and the concentration of nitrate nitrogen, nitrite nitrogen and total nitrogen in water entering and exiting a reactor are measured every period, and after the degradation of the nitrate nitrogen and the total nitrogen of the activated sludge system is stable, calculating a period; the first period achieves the effect of stability, namely the degradation of nitrate nitrogen and total nitrogen of the activated sludge system corresponding to the first period and the subsequent continuous 3 periods is stable and consistent, which indicates that the sludge system has high strengthening and domestication speed and strong stability;
under the condition of low temperature in winter, the COD concentration of the inlet water is 480-600 mg/L, the nitrate nitrogen concentration is 60mg/L, and the carbon nitrogen ratio of the inlet water is 8-10.
2. The method of claim 1, wherein the first cycle of the reactor is sampled at full cycle in step 3) to determine nitrate nitrogen and total nitrogen concentration, which is analyzed to enhance the biological denitrification performance of the activated sludge system.
3. The method according to claim 1, wherein the nitrate waste water used in step 2) and step 3) is configured manually with NaNO 3 Is a nitrogen source, CH 3 COONa is carbon source, KH 2 PO 4 Is a phosphorus source, and trace elements are 1ml/L; the nitrate nitrogen concentration is 60mg/L, the COD concentration is 480-600 mg/L, the carbon-nitrogen ratio of the inlet water is 8-10, and the composition and the concentration of the trace element solution are as follows: na (Na) 2 EDTA 4.29g/L, feCl 2 ·4H 2 O is 1.99g/L, mnCl 2 ·2H 2 O is 0.08g/L, niCl 2 ·6H 2 O is 0.02g/L, coCl 2 ·6H 2 O is 0.02g/L, cuCl 2 ·H 2 O is 0.02g/L, znCl 2 0.02g/L NaMoO 4 ·2H 2 O is 0.02g/L, na 2 WoO 4 ·2H 2 O is 0.03g/L, H 3 BO 3 0.06g/L.
4. The method according to claim 1, wherein step 3) is operated under the same conditions as step 2) as: the concentration of the activated sludge is 4g/L, the volume of the activated sludge and nitrate wastewater is 1:1, the drainage ratio is 50%, the low-temperature condition is 13-15 ℃, the dissolved oxygen DO is less than or equal to 0.2mg/L, and the rotating speed of the constant-temperature vibrating box is 110-120 rad/min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310325514.3A CN116199334B (en) | 2023-03-29 | 2023-03-29 | Method for strengthening biological denitrification efficiency of low-temperature activated sludge by chlorophyll |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310325514.3A CN116199334B (en) | 2023-03-29 | 2023-03-29 | Method for strengthening biological denitrification efficiency of low-temperature activated sludge by chlorophyll |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116199334A CN116199334A (en) | 2023-06-02 |
| CN116199334B true CN116199334B (en) | 2024-03-12 |
Family
ID=86514798
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310325514.3A Active CN116199334B (en) | 2023-03-29 | 2023-03-29 | Method for strengthening biological denitrification efficiency of low-temperature activated sludge by chlorophyll |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116199334B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115140847B (en) * | 2022-07-05 | 2023-11-03 | 南京大学 | Mediator-enhanced wastewater deep biological denitrification method |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2299328A1 (en) * | 1998-06-18 | 1999-12-23 | Boris Mikhailovich Khudenko | Mediated biological-abiotic waste treatment |
| JP2014046257A (en) * | 2012-08-31 | 2014-03-17 | Jfe Steel Corp | Biological treatment method of nitrogen-containing waste water |
| CN104478084A (en) * | 2014-12-08 | 2015-04-01 | 天津城建大学 | Method for enhancing biological denitrification and denitrification of low-temperature sewage in winter |
| CN108033551A (en) * | 2017-12-11 | 2018-05-15 | 天津城建大学 | The method for improving low-temperature sewage biological denitrification dephosphorization |
| CN111410307A (en) * | 2020-05-12 | 2020-07-14 | 知和环保科技有限公司 | Low-temperature-resistant biological membrane denitrification process |
| CN111454859A (en) * | 2020-04-07 | 2020-07-28 | 山东海景天环保科技股份公司 | Method for cultivating low-temperature aerobic denitrifying bacteria |
| CN114506924A (en) * | 2022-01-25 | 2022-05-17 | 北京工业大学 | Method for rapidly realizing low-carbon-consumption synchronous nitrification and denitrification efficient denitrification |
-
2023
- 2023-03-29 CN CN202310325514.3A patent/CN116199334B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2299328A1 (en) * | 1998-06-18 | 1999-12-23 | Boris Mikhailovich Khudenko | Mediated biological-abiotic waste treatment |
| JP2014046257A (en) * | 2012-08-31 | 2014-03-17 | Jfe Steel Corp | Biological treatment method of nitrogen-containing waste water |
| CN104478084A (en) * | 2014-12-08 | 2015-04-01 | 天津城建大学 | Method for enhancing biological denitrification and denitrification of low-temperature sewage in winter |
| CN108033551A (en) * | 2017-12-11 | 2018-05-15 | 天津城建大学 | The method for improving low-temperature sewage biological denitrification dephosphorization |
| CN111454859A (en) * | 2020-04-07 | 2020-07-28 | 山东海景天环保科技股份公司 | Method for cultivating low-temperature aerobic denitrifying bacteria |
| CN111410307A (en) * | 2020-05-12 | 2020-07-14 | 知和环保科技有限公司 | Low-temperature-resistant biological membrane denitrification process |
| CN114506924A (en) * | 2022-01-25 | 2022-05-17 | 北京工业大学 | Method for rapidly realizing low-carbon-consumption synchronous nitrification and denitrification efficient denitrification |
Non-Patent Citations (2)
| Title |
|---|
| Chlorophyll as natural redox mediators for the denitrification process;Caicai Lu et al.;《International Biodeterioration & Biodegradation》;第1-7页 * |
| 不同碳源对低温投加氧化还原介体污水生物反硝化脱氮过程的影响;苑宏英;孙烨怡;李原玲;孙锦绣;王小佩;;化工进展;20180205(第02期);第370-375页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN116199334A (en) | 2023-06-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Shen et al. | A study of ferric-carbon micro-electrolysis process to enhance nitrogen and phosphorus removal efficiency in subsurface flow constructed wetlands | |
| Yuan et al. | Woodchips as sustained-release carbon source to enhance the nitrogen transformation of low C/N wastewater in a baffle subsurface flow constructed wetland | |
| CN110615531B (en) | DEAMOX sludge double-reflux AOAO sewage-based deep nitrogen and phosphorus removal device and method | |
| Lu et al. | Simultaneously enhanced removal of PAHs and nitrogen driven by Fe2+/Fe3+ cycle in constructed wetland through automatic tidal operation | |
| CN101830558B (en) | Method for cultivating anaerobic ammonium oxidation granular sludge | |
| CN102181421A (en) | Method for strengthening activity of anaerobic ammoxidized microorganisms through polyvinyl alcohol-sodium alginate-activated carbon embedment | |
| Ahmadi et al. | Kinetic study and performance evaluation of an integrated two-phase fixed-film baffled bioreactor for bioenergy recovery from wastewater and bio-wasted sludge | |
| CN116199334B (en) | Method for strengthening biological denitrification efficiency of low-temperature activated sludge by chlorophyll | |
| CN102533623B (en) | Achromobacter xylosoxidans with denitrification and dephosphorization function and application of Achromobacter xylosoxidans | |
| CN112250176A (en) | Device and method for realizing advanced nitrogen and phosphorus removal of municipal sewage by integrated shortcut nitrification coupled with anaerobic ammonia oxidation denitrification phosphorus removal | |
| Debik et al. | Sequence optimization in a sequencing batch reactor for biological nutrient removal from domestic wastewater | |
| Feng et al. | Enhanced nutrient removal from mainstream sewage via denitrifying dephosphatation, endogenous denitrification and anammox in a novel continuous flow process | |
| Liu et al. | Synergistic partial denitrification, anammox and in-situ fermentation (SPDAF) process for treating domestic and nitrate wastewater: Response of nitrogen removal performance to decreasing temperature | |
| Li et al. | Stable enhanced nitrogen removal from low COD/N municipal wastewater via partial nitrification-anammox in the traditional continuous anoxic/oxic process through bio-augmentation of partial nitrification sludge under decreasing temperatures | |
| Zhang et al. | Ultra-low energy consumption process (PN+ Anammox) for enhanced nitrogen removal from decentralized sewage | |
| Wang et al. | Two-stage hybrid microalgal electroactive wetland-coupled anaerobic digestion for swine wastewater treatment in South China: Full-scale verification | |
| Lin et al. | Autotrophic nitrogen removal by partial nitrification-anammox process in two-stage sequencing batch constructed wetlands for low-strength ammonium wastewater | |
| CN103387289B (en) | Method for strengthening azo dye biodegradation by utilizing zero-valent iron | |
| CN105461080A (en) | Method for removing ammonia nitrogen and total nitrogen in tail water with bacterial agent strengthened subsurface flow wetland | |
| Pang et al. | Effect of ferric iron (Fe (Ш)) on heterotrophic solid-phase denitrification: Denitrification performance and metabolic pathway | |
| CN103074286B (en) | High-salt heterotrophic nitrification-aerobic denitrification dephosphorization salinivibrio and application of salinivibrio in wastewater treatment | |
| CN109231478B (en) | Starting method of all-biological phosphorus removal AOO process | |
| Sun et al. | Effects of matrix modification and bacteria amendment on the treatment efficiency of municipal tailwater pollutants by modified vertical flow constructed wetland | |
| Luo et al. | Initiating an anaerobic ammonium oxidation reactor by inoculation with starved anaerobic ammonium oxidation sludge and modified carriers | |
| CN103074285B (en) | High-salt heterotrophic nitrification-aerobic denitrification dephosphorization brachybacterium and application of brachybacterium in wastewater treatment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |