CN105461080A - Method for removing ammonia nitrogen and total nitrogen in tail water with bacterial agent strengthened subsurface flow wetland - Google Patents
Method for removing ammonia nitrogen and total nitrogen in tail water with bacterial agent strengthened subsurface flow wetland Download PDFInfo
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- CN105461080A CN105461080A CN201510827970.3A CN201510827970A CN105461080A CN 105461080 A CN105461080 A CN 105461080A CN 201510827970 A CN201510827970 A CN 201510827970A CN 105461080 A CN105461080 A CN 105461080A
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- tail water
- wetland
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- nitrogen
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
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Abstract
The invention discloses a method for removing ammonia nitrogen and total nitrogen in tail water with a bacterial agent strengthened subsurface flow wetland, and belongs to the technical field of water treatment. A B8 preparation is added in a subsurface flow constructed wetland, is allowed to coexist with biofilms of the surface and pores of a wetland substrate, and becomes dominant flora, so that a nitrogen removal function of a wetland system is strengthened, the concentration of the ammonia nitrogen and the total nitrogen in the tail water can be effectively reduced, and the problem of low nitrogen removal efficiency of the subsurface flow wetland on treatment of tail water with low carbon source and containing nitrogen is overcome. The method has the characteristics of low investment, low cost, stable treatment effect, convenient operation and management and the like.
Description
Technical field
The invention belongs to water-treatment technology field, be specifically related to the method that a kind of microbial inoculum strengthening current wetland removes ammonia nitrogen and total nitrogen in tail water.
Background technology
In recent years, Some Domestic sewage treatment plant tail water enters neighbouring Hu Tang, river course nearby, causes surface water body water pollution, and body eutrophication phenomenon is day by day serious.A kind of sewage disposal technology that horizontal drowned flow artificial wet land is collaborative soil, filtrate, plant are purified water by physics, chemistry, biological action, is applied to sewage treatment plant tail water advanced treatment mostly.But be in anaerobic environment for a long time bottom current wetland, and the tail water entering wet land system is compared with general city and domestic sewage in rural areas, the COD concentration of tail water is lower namely lower for biodegradable carbon source, this anaerobism and low-carbon-source environment hinder the flourish of denitrifying bacterium in wetland inside, ammonia nitrogen and nitric nitrogen are stranded in water in a large number, thus make ammonia nitrogen and nitrogen removal rate lower.In order to solve this difficult problem, Chinese scholars proposes multiple solution, roughly can be classified as two large classes: a class strengthens the inner reoxygenation of subsurface flow wetland system, such as, bottom wetland, set up air lead or the electrolysis of installing electrodes plate produces oxygen; One class selects the absorption of efficient adsorption stuffing enhancing to nitrogen, such as ferric oxide, slag.But it is still lower that above method improves denitrification efficiency of constructed wetland, and need to increase energy consumption and change filler etc. to operate comparatively loaded down with trivial details.
Denitrifying bacterium is under microbial enzyme effect, to reduce nitrate, discharges molecular nitrogen (N
2) or nitrous oxide (N
2o) a kind of microorganism with denitrification function.Publication number is the patent of invention report of CN102757132A, reed associated treatment domestic sewage in rural areas in underflow type artificial wet land that Pei Haiyan etc. utilize Denitrifying Phosphate Accumulating Organisms CL-3 (Pseudomonasoleovorans) abundant with rhizospheric microorganism, makes system have good removal effect to COD, ammonia nitrogen and total phosphorus.The strain obtained by this laboratory is had the pseudomonas putida B8 (Pseudomonasputida of aerobic dephosphorization and anoxic denitrification ability by the present inventor, this bacterial strain preserving number is CGMCC9168) obtained bacteria preparation is added in Horizontal subsurface constructed wetland system, it can coexist with the microbial film in Wetland Substrate surface and hole thereof, and become dominant microflora, and then the denitrification functions of strengthening wet land system, thus ammonia nitrogen and total nitrogen concentration in low-carbon-source tail water can be effectively reduced.
Summary of the invention
The object of the invention is to overcome current wetland process low-carbon-source, nitrogenous tail water denitrification effect this difficult problem not high, and provide a kind of microbial inoculum to strengthen the method for ammonia nitrogen and total nitrogen in current wetland removal tail water.Namely in underflow type artificial wet land, add B8 preparation, the microbial film in itself and Wetland Substrate surface and hole thereof is coexisted, and becomes dominant microflora, and then the denitrification functions of strengthening wet land system, thus ammonia nitrogen and total nitrogen concentration in tail water can be effectively reduced.
The technical solution used in the present invention is:
1, the tail water that municipal sewage plant reaches " urban wastewater treatment firm pollutant emission standard " (GB18918-2002) one-level B water quality standard enters subsurface flow wetland system as pending waste water.
2, add in Horizontal subsurface constructed wetland system by B8 preparation, its concrete operations are: by B8 preparation, (microbial inoculum viable count is 6.25 × 10
7~ 8.55 × 10
7cfu/mL) (2 days are added, process tail water throwing bacterium amount 1.25 × 10 with 0.2% of the pending tail water water yield (V/V) successively
11~ 1.71 × 10
11cfu/m
3), 0.4% (add 2 days, process tail water throws bacterium amount 2.50 × 10
11~ 3.42 × 10
11cfu/m
3), 0.8% (add 2 days, process tail water throws bacterium amount 5.00 × 10
11~ 6.84 × 10
11cfu/m
3), 1.0% (add 2 days, process tail water throws bacterium amount 6.26 × 10
11~ 8.56 × 10
11cfu/m
3), 2.0% (add 6 days, process tail water throws bacterium amount 1.25 × 10
12~ 1.71 × 10
12cfu/m
3) mixed with tail water by volume pump after, allow its continuous 14 days and flow in Horizontal subsurface constructed wetland, thus the microbial film in the surperficial and hole of B8 and Wetland Substrate is coexisted, and become dominant microflora.
3, monitor wet land system denitrification effect after stopping throwing bacterium, concrete operations are: adopt the emptying operational mode of water inlet-wetland deep purifying 24h-to run, the interior mensuration water inlet of 8h, effluent quality (monitoring index has ammonia nitrogen and total nitrogen) after sampling.
The invention provides 1 strain bacterial strain, its called after B8, be accredited as Pseudomonas putida Pseudomonas (Pseudomonasputida) through 16SrDNA order-checking and tetraploid rice, be stored in the China Committee for Culture Collection of Microorganisms's (register on the books and be numbered: CGMCC9168) being positioned at No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City on May 16th, 2014.Utilize above-mentioned bacterial classification to prepare B8 preparation, its preparation method has been carried out concrete describing in the patent of CN201410472759.X in the patent No., and the present invention refer to microbial inoculum prepared by aforesaid method.
Cardinal principle of the present invention:
Introduced by B8 in subsurface flow wetland system, intaking-to process-emptying mode runs, particularly air effectively can be introduced wetland bed inside by water inlet and emptying operation, impel B8 can in wetland growth and reproduction.B8 can under anoxic conditions, and be used for reducing nitrate by microbial enzyme, discharge molecular nitrogen or nitrous oxide, its denitrification process can be expressed as with equation: 2NO
3 -+ 10e
-+ 12H
+→ N
2+ 6H
2o, and B8 is in anaerobic environment, ammonium ion nitrite anions can be oxidized to nitrogen, be expressed as: NH with equation
4 ++ NO
2 -→ N
2+ 2H
2o.
The present invention has following advantage:
(1) add to Horizontal subsurface constructed wetland the removal effect that B8 preparation effectively improves ammonia nitrogen in sewage and total nitrogen.
(2) the present invention can slow down the frequency changing filler, and operational management is convenient, effectively reduces investment outlay.
(3) bacterial classification of the B8 preparation of the present invention's use derives from active sludge in the oxidation ditch outer fissure of sewage work of Tianzhang City of Anhui Province, and its process for sewage treatment plant tail water has certain adaptability.
(4) low-carbon-source (" urban wastewater treatment firm pollutant emission standard " GB18918-2002 one-level B water quality standard) up to standard tail water is strengthened by microbial inoculum, reach the preferably removal effect to ammonia nitrogen in tail water and total nitrogen, make ecological method advanced treatment tail water become possibility.
Embodiment
A kind of microbial inoculum strengthening current wetland of the present invention removes the preparation of method by B8 preparation, steady running 3 step connecing bacterium, artificial swamp of artificial swamp of ammonia nitrogen and total nitrogen in tail water, is now described below respectively:
1, the preparation of B8 microbial inoculum
The pseudomonas putida B8 of slant medium is hidden in sterilized vaccinating pen picking 1 environmental protection, be inoculated in the 100mL Erlenmeyer flask of the 50mLDNJPU substratum that pH6.5 after high-temp steam sterilizing is housed, in shaking table, (culture temperature 30 DEG C is set, shaking speed 120r/min) rock and cultivate 20h, (microbial inoculum viable count is 6.25 × 10 to obtain B8 preparation
7~ 8.55 × 10
7cfu/mL).
2, artificial swamp connect bacterium
The tail water of one-level B water quality standard that municipal sewage plant reaches " urban wastewater treatment firm pollutant emission standard " (GB18918-2002) enters subsurface flow wetland system process as pending waste water.Add in Horizontal subsurface constructed wetland system by obtained B8 preparation, its concrete operations are: B8 preparation (is added 2 days, process tail water throwing bacterium amount 1.25 × 10 with 0.2% of the pending tail water water yield (V/V) successively
11~ 1.71 × 10
11cfu/m
3), 0.4% (add 2 days, process tail water throws bacterium amount 2.50 × 10
11~ 3.42 × 10
11cfu/m
3), 0.8% (add 2 days, process tail water throws bacterium amount 5.00 × 10
11~ 6.84 × 10
11cfu/m
3), 1.0% (add 2 days, process tail water throws bacterium amount 6.26 × 10
11~ 8.56 × 10
11cfu/m
3), 2.0% (add 6 days, process tail water throws bacterium amount 1.25 × 10
12~ 1.71 × 10
12cfu/m
3) mixed with tail water by volume pump after, allow its continuous 14 days and flow in Horizontal subsurface constructed wetland system front end distribution reservoir, then allow water body from flowing to after current wetland stops 24h, then allow water body discharge.Thus the microbial film in B8 and Wetland Substrate surface and hole thereof is coexisted, and become dominant microflora, obtain B8 microbial inoculum strengthening subsurface flow wetland system.
3, the steady running of artificial swamp
Artificial swamp connect bacterium end of operation after, subsurface flow wetland system is strengthened to B8 and carries out monitoring with microbial inoculum subsurface flow wetland system denitrification effect and contrast, concrete operations are: all adopt operational mode to be that the emptying artificial wet land system of water inlet-wetland deep purifying 24h-carries out deep purifying to sewage treatment plant tail water, measure in 8h after sampling intake, effluent quality (monitoring index has ammonia nitrogen and total nitrogen).
4 embodiments are below provided to further illustrate the present invention.
Embodiment 1
The present embodiment is disposed of sewage treatment plant tail water 12m
3/ d.Artificial swamp (wet land filler porosity is 30.75%) connect bacterium end of operation after, run B8 preparation strengthening subsurface flow wetland system after 6 days, monitor its denitrification effect, concrete operations are: adopt operational mode to be that the emptying artificial wet land system of water inlet-wetland deep purifying 24h-carries out deep purifying to sewage treatment plant tail water.
Table 1 subsurface flow wetland system structure, operation and water quality treatment situation
Embodiment 2
The present embodiment is disposed of sewage treatment plant tail water 21m
3/ d.Artificial swamp (wet land filler porosity is 34.34%) connect bacterium end of operation after, run B8 preparation strengthening subsurface flow wetland system after 19 days, monitor its denitrification effect, concrete operations are: adopt operational mode to be that the emptying artificial wet land system of water inlet-wetland deep purifying 24h-carries out deep purifying to sewage treatment plant tail water.
Table 2 subsurface flow wetland system structure, operation and water quality treatment situation
Embodiment 3
The present embodiment is disposed of sewage treatment plant tail water 56m
3/ d.Artificial swamp (wet land filler porosity is 33.67%) connect bacterium end of operation after, run B8 preparation strengthening subsurface flow wetland system after 23 days, monitor its denitrification effect, concrete operations are: adopt operational mode to be that the emptying artificial wet land system of water inlet-wetland deep purifying 24h-carries out deep purifying to sewage treatment plant tail water.
Table 3 subsurface flow wetland system structure, operation and water quality treatment situation
Embodiment 4
The present embodiment is disposed of sewage treatment plant tail water 84m
3/ d.Artificial swamp (wet land filler porosity is 33.19%) connect bacterium end of operation after, run B8 preparation strengthening subsurface flow wetland system after 41 days, monitor its denitrification effect, concrete operations are: adopt operational mode to be that the emptying artificial wet land system of water inlet-wetland deep purifying 24h-carries out deep purifying to sewage treatment plant tail water.
Table 4 subsurface flow wetland system structure, operation and water quality treatment situation
Claims (3)
1. the method that current wetland removes ammonia nitrogen and total nitrogen in tail water strengthened by a microbial inoculum, it is characterized in that: obtained B8 preparation is added in Horizontal subsurface constructed wetland system, after being mixed with tail water by volume pump by B8 preparation and the pending tail water water yield, allow its continuous 14 days and flow in Horizontal subsurface constructed wetland system front end distribution reservoir, then allow water body after flowing to current wetland stop 24h, then allow water body discharge thus to carry out startup optimization.After the startup of artificial swamp, subsurface flow wetland system is strengthened to B8 and carries out monitoring with microbial inoculum subsurface flow wetland system denitrification effect and contrast, adopt operational mode to be that the emptying artificial wet land system of water inlet-wetland deep purifying 24h-carries out deep purifying to sewage treatment plant tail water again, after sampling, in 8h, measure water inlet, effluent quality index.
2. a kind of microbial inoculum strengthening current wetland according to claim 1 removes the start-up operation of the method for ammonia nitrogen and total nitrogen in tail water, it is characterized in that: B8 preparation (is added 2 days, process tail water throwing bacterium amount 1.25 × 10 with 0.2% of the pending tail water water yield (V/V) successively
11~ 1.71 × 10
11cfu/m
3), 0.4% (add 2 days, process tail water throws bacterium amount 2.50 × 10
11~ 3.42 × 10
11cfu/m
3), 0.8% (add 2 days, process tail water throws bacterium amount 5.00 × 10
11~ 6.84 × 10
11cfu/m
3), 1.0% (add 2 days, process tail water throws bacterium amount 6.26 × 10
11~ 8.56 × 10
11cfu/m
3), 2.0% (add 6 days, process tail water throws bacterium amount 1.25 × 10
12~ 1.71 × 10
12cfu/m
3) mixed with tail water by volume pump after, allow its continuous 14 days and flow in Horizontal subsurface constructed wetland system front end distribution reservoir, then allow water body from flowing to after current wetland stops 24h, then allow water body discharge.
3. B8 microbial inoculum according to claim 1, its feature is carried out according to following step: the pseudomonas putida B8 being hidden in slant medium with sterilized vaccinating pen picking 1 environmental protection, be inoculated in the 100mL Erlenmeyer flask of the 50mLDNJPU substratum that pH6.5 after high-temp steam sterilizing is housed, in shaking table, (culture temperature 30 DEG C is set, shaking speed 120r/min) rock and cultivate 20h, (microbial inoculum viable count is 6.25 × 107 ~ 8.55 × 10 to obtain B8 preparation
7cfu/mL).
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|---|---|---|---|
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|---|---|---|---|
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105858896A (en) * | 2016-04-15 | 2016-08-17 | 农业部环境保护科研监测所 | Rural domestic sewage artificial wetland system for carrying out reinforced denitrification and purifying method |
| CN106045217A (en) * | 2016-07-20 | 2016-10-26 | 申昱环保科技股份有限公司 | Biological combined treatment method for rural domestic sewage |
| CN106045060A (en) * | 2016-07-20 | 2016-10-26 | 申昱环保科技股份有限公司 | Method for purifying domestic sewage by combination of granular multiple species inoculant and water plants |
| CN107055950A (en) * | 2017-03-13 | 2017-08-18 | 常州大学 | The advanced purification process and device of a kind of waste emulsified mixture |
| CN109455873A (en) * | 2018-10-11 | 2019-03-12 | 常州大学 | A method of village initial rainwater is purified using the drainage ditch in southern village |
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| CN103979736A (en) * | 2014-05-21 | 2014-08-13 | 南京大学 | Artificial wetland device for denitrification of low-pollution water and treatment method thereof |
| CN104212748A (en) * | 2014-09-17 | 2014-12-17 | 常州大学 | Denitrifying phosphorus accumulation bacterial strain and method for removing nitrate nitrogen and total phosphorus in wastewater by utilizing denitrifying phosphorus accumulation bacterial strain |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105858896A (en) * | 2016-04-15 | 2016-08-17 | 农业部环境保护科研监测所 | Rural domestic sewage artificial wetland system for carrying out reinforced denitrification and purifying method |
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| CN106045060A (en) * | 2016-07-20 | 2016-10-26 | 申昱环保科技股份有限公司 | Method for purifying domestic sewage by combination of granular multiple species inoculant and water plants |
| CN107055950A (en) * | 2017-03-13 | 2017-08-18 | 常州大学 | The advanced purification process and device of a kind of waste emulsified mixture |
| CN109455873A (en) * | 2018-10-11 | 2019-03-12 | 常州大学 | A method of village initial rainwater is purified using the drainage ditch in southern village |
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