CN116195499A - Method for controlling lily stamen petal and application thereof - Google Patents
Method for controlling lily stamen petal and application thereof Download PDFInfo
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- 241000234435 Lilium Species 0.000 title claims abstract description 76
- 238000000034 method Methods 0.000 title claims abstract description 36
- 238000011282 treatment Methods 0.000 claims abstract description 43
- 241000196324 Embryophyta Species 0.000 claims abstract description 10
- 238000005286 illumination Methods 0.000 claims description 24
- 239000002689 soil Substances 0.000 claims description 20
- 239000003814 drug Substances 0.000 claims description 17
- 239000003337 fertilizer Substances 0.000 claims description 11
- 229940079593 drug Drugs 0.000 claims description 6
- 230000036244 malformation Effects 0.000 claims description 6
- 239000011159 matrix material Substances 0.000 claims description 6
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 5
- 239000003415 peat Substances 0.000 claims description 4
- 238000005057 refrigeration Methods 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 238000001816 cooling Methods 0.000 abstract description 3
- 238000005265 energy consumption Methods 0.000 abstract description 3
- 230000001276 controlling effect Effects 0.000 description 10
- 239000000243 solution Substances 0.000 description 7
- 239000007864 aqueous solution Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241001573881 Corolla Species 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000004781 supercooling Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000234280 Liliaceae Species 0.000 description 1
- 241001656752 Lilium philadelphicum Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
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- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
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- 210000000056 organ Anatomy 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention provides a method for controlling lily stamen flap and application thereof, and relates to the technical field of plant cultivation, wherein the method comprises the steps of treating lily by adjusting temperature, then carrying out growth management on the lily until the lily flowers, and identifying the stamen flap degree of the lily so as to control the lily stamen flap; the stamen petal of the lily can be controlled by temperature. The invention reduces the instability of the flower heavy petal phenotype of the product, improves the commercial value of the product, reduces the energy consumption caused by excessive cooling or whole-growth-period cooling treatment and avoids excessive cost investment by determining and controlling the temperature range and the temperature treatment time of lily red double happiness stamen petal. The invention also determines the identification standard of lily stamen petal grade, and provides a basis for grading the quality of lily fresh flowers.
Description
Technical Field
The invention belongs to the technical field of plant cultivation, and particularly relates to a method for controlling lily stamen petal and application thereof.
Background
The lily is a perennial bulb flower of the family Liliaceae, belongs to a variety of the field lily, is a health food and a common traditional Chinese medicine for both medicine and food, and has ornamental value. The flower is large in the international fresh flower market, the flower appearance is elegant, the flower color is colorful, the application is various (can be used for potted flowers, cut flowers, garden application and the like), and the flower is popular. The wild lily is generally a single petal flower and is provided with four-wheel flower organs, namely an outer petal, an inner petal, a stamen and a pistil from outside to inside. The number of petals of the heavy-petal lily is large, the total inflorescence is longer than the top of the stem, the corolla is funnel-shaped, the fine flower of the corolla is full, and a plurality of layers of petals are arranged on a single flower of the heavy-petal lily, generally, the petals are increased by showing the petaled stamens and even the petaled pistils, and the heavy-petal lily has higher commercial value due to the more attractive appearance and the polleless characteristic.
Most lily flowers exhibit stable single-or double-petal characteristics. However, some heavy-petal varieties exhibit single-petal characteristics in some cases for unknown reasons, such as the lily varieties "red double happiness", "twin gold", "erlodi", etc., affecting the uniformity of the flower product phenotype. The lily is a orange red double happiness lily variety, the double petal characteristic of the lily is derived from the petal of stamen, and in most cases, the stamen can be converted into arrow-shaped petals, the posture is peculiar, and the commercial value is extremely high. However, in some circumstances "Hongshuang" flowers, the stamens cannot be converted to petals, but rather form normal stamens and can produce pollen, greatly reducing its commercial value. Therefore, to change this phenomenon, many scholars have aimed at promoting the development of stamen valve. Patent application CN105367189a discloses a liquid medicine for promoting lily stamen to be petalonized, through spraying the liquid medicine on lily stamen, the stamen can be petalonized, partial pollen disappears or becomes small, the pollen quantity is reduced, petals are increased, the problem that lily stamen cannot be converted into petals can be solved, but the liquid medicine is composed of various chemical substances, the growth of lily per se can be influenced, and even environmental pollution is serious. And to date, there has been no related study to identify the cause of lily exhibiting univalvular properties and to provide a simple method to improve this property.
Therefore, there is a need for a method that can control lily stamen valve, is simple in control process, and can identify the degree of stamen valve.
Disclosure of Invention
As used herein, the singular forms "a," "an," and "the" include the singular and plural referents unless the context clearly dictates otherwise. The recitation of numerical ranges by endpoints includes all numbers and fractions subsumed within that corresponding range, and the endpoints recited.
Aiming at the problems existing in the prior art, the invention provides a method for controlling lily stamen to be valved and application thereof, and the instability of the flower weight petal phenotype is reduced and the commercial value is improved by controlling the temperature treatment method for lily to be highly valved.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
the invention provides a method for controlling lily stamen petal, which comprises the steps of treating lily by adjusting temperature, then carrying out growth management on the lily until the lily flowers, and identifying the degree of lily stamen petal so as to control lily stamen petal; the stamen petal of the lily can be controlled by temperature.
Preferably, the temperature is 14-22 ℃.
Further preferably, the temperature is 20-22 ℃.
Most preferably, the temperature is 22 ℃.
Preferably, the step of growth management comprises treatment of light duration, application of water-soluble fertilizer.
Preferably, the illumination time is 10-16h.
Preferably, the step of growth management further comprises a treatment of illumination intensity.
Specifically, the illumination intensity is 15000Lx.
Preferably, the application of the water-soluble fertilizer is a 1000-fold dilution of the water-soluble fertilizer, and the water-soluble fertilizer comprises 'flower number 1' and/or 'flower number 2'.
Preferably, the identification method comprises the following steps: the average value of the stamen valvulation of the plants under the same planting condition is the stamen valvulation value under the condition, and the valvulation degree is judged according to the valvulation value, and the method specifically comprises the following steps:
the stamen valve value is more than or equal to 4, so that the stamen valve degree is high;
the stamen valve value is more than 1 and less than 4, and the stamen valve degree is moderate;
the stamen valve value is less than or equal to 1, and the stamen valve degree is low.
Preferably, the method comprises the following steps:
s1, preparing materials: preparing lily bulbs which can bloom normally after refrigeration treatment, wherein the diameter of the bulbs is 14-20cm, peat is used as a matrix, the specification of the matrix is 10-40mm, the thickness of the covering soil is 2 times of the height of the bulbs, the planting temperature is 14-28 ℃, and water-soluble fertilizer is applied after the soil surface is dried, and the bulbs are planted until top buds of the lily are planted;
s2, temperature treatment: transferring the lily with terminal bud soil into a planting environment with the temperature of 14-22 ℃, controlling the illumination intensity to 15000-80000Lx and the illumination duration to 10-16h, and applying water-soluble fertilizer after the soil surface is dried, and performing temperature treatment;
s3, growth management: transferring the lily subjected to the S2 treatment into a planting environment at 14-28 ℃, controlling the illumination intensity to 15000-80000Lx and the illumination duration to 10-16h, and applying a water-soluble fertilizer after the soil surface is dried until flowering;
s4, assigning a stamen valve value: performing valvulation value assignment on stamens of the lily after flowering;
s5, identifying the degree of stamen valve: the average value of the stamen valvulation of the plants under the same planting condition is the stamen valvulation value under the condition, and the valvulation degree is judged according to the valvulation value, and the method specifically comprises the following steps:
the stamen valve value is more than or equal to 4, so that the stamen valve degree is high;
the stamen valve value is more than 1 and less than 4, and the stamen valve degree is moderate;
the stamen valve value is less than or equal to 1, and the stamen valve degree is low.
Preferably, the height of the terminal bud in the step S1 is not more than 2 times of that of the lily bulbs when the terminal bud is unearthed.
Preferably, the time of the treatment in the step S2 is from the time when the terminal bud is earthed to the time when the minimum flower bud grows to 3 mm.
Preferably, the assigning method in step S4 is as follows: the degree of valvulation of the stamen was classified into 6 grades, and the degree of valvulation was assigned 0 to 5 from low to high, respectively.
Preferably, the 6 grades are specifically: level 0, 4 stamen chambers, more pollen and filigree shape; 1 grade, 2 stamen chambers, less pollen and filigree shape; grade 2, malformation of stamen and medicine room, no pollen and filigree shape; grade 3, stamen drug chamber malformation, no pollen, basal petal of filigree; 4, the stamens have no medicine room and filament shape; grade 5, stamen without drug chamber and filament basal petal.
The invention also provides an application of the method in controlling or improving lily stamen petal.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention determines the optimal temperature range of 14-22 ℃ for forming the height valve of the stamen by the lily with the temperature regulated and controlled by the stamen valve, and the specific temperature can be regulated within the range of 14-22 ℃ according to the temperature-controlled energy consumption condition, thereby not only reducing the instability of the heavy valve phenotype of the lily, but also avoiding the energy consumption increase caused by excessive cooling;
2. the invention determines the time of temperature treatment as the length from the top bud to the minimum flower bud growing to 3mm after the top bud is unearthed, and the range is the key stage of temperature treatment, so that excessive cost investment can be avoided;
3. the invention determines the identification standard of the stamen petal grade, provides a basis for grading the quality of the fresh lily, and improves the commercial value of the lily.
Drawings
FIG. 1 is a morphology of "Hongshuangxi" lily, wherein a is a morphology with low degree of stamen valve, and b is a morphology with high degree of stamen valve.
Fig. 2 is a morphology diagram of stamen with different degrees of petal of "red double happiness" lily, wherein fig. 0, 1, 2, 3, 4, 5 show morphology diagrams of petal degree grades 1, 2, 3, 4, 5, 6, respectively.
FIG. 3 is a statistical plot of lily "Hongshuangxi" stamen flap values under different constant temperature treatments.
Fig. 4 is a statistical chart of lily "red double happiness" stamen valve values under different temperature changing treatments, wherein a is a chart of different temperature changing treatments, and b is a corresponding stamen valve value under different temperature changing treatments.
Detailed Description
The following description of the present invention is provided by way of specific examples to facilitate understanding and grasping of the technical solution of the present invention, but the present invention is not limited thereto, and the described examples are only some, but not all, examples of the present application.
The endpoints and any values of the ranges disclosed herein are not limited to the precise range or value, and are understood to encompass values approaching those ranges or values. For numerical ranges, one or more new numerical ranges may be found between the endpoints of each range, between the endpoint of each range and the individual point value, and between the individual point value, in combination with each other, and are to be considered as specifically disclosed herein.
All other embodiments, which can be made by one of ordinary skill in the art based on the embodiments herein without making any inventive effort, shall fall within the scope of the present application. The experimental methods described in the following examples are all conventional methods unless otherwise specified; the reagents and materials, unless otherwise specified, are commercially available.
Example 1
The lily bulb (English name: red twin) in the embodiment is purchased from Netherlands, is a bulb which can be normally opened after supercooling and storing treatment, is subjected to planting tests under different constant temperature treatments, and the stamen flap degree is identified, and the specific steps are as follows:
performing valve value assignment on stamens of all flowers in a red double happiness single plant;
the stamen valve value assignment method comprises the following steps: based on morphological description of different stamen valve values in the specification, valve value assignment is carried out on each stamen, the valve degree of each stamen is divided into 6 grades, and valve degree is respectively assigned to 0-5 from low to high; level 0, 4 stamen chambers, more pollen and filigree shape; 1 grade, 2 stamen chambers, less pollen and filigree shape; grade 2, malformation of stamen and medicine room, no pollen and filigree shape; grade 3, stamen drug chamber malformation, no pollen, basal petal of filigree; 4, the stamens have no medicine room and filament shape; grade 5, stamen without drug chamber and filament basal petal. The specific hierarchical morphology is shown in figure 2.
Step 5: identifying the degree of stamen valve:
calculating the average value of the stamen valve value of each lily as the stamen valve value of the lily; the average value of the stamen valve of 6 plants under the same temperature treatment condition is the stamen valve under the condition; the calculation result is shown in fig. 3;
according to the stamen valve value, the stamen valve degree under different temperature treatment conditions is identified, and the specific steps are as follows:
the valve value of stamen is more than 4 under the treatment of 14 ℃ and 22 ℃ and the valve degree is not changed greatly, so the valve degree of stamen is high,
has great commercial value;
the stamen valve value is more than 1 and less than 4 under the treatment of 24 ℃ and 26 ℃, so that the stamen valve degree is medium, and the commercial value is medium.
The stamen valve value under 28 ℃ treatment is 0, the stamen valve degree is low, and the commercial value is low.
Example 2
The lily bulb (English name: red twins) in the embodiment is purchased from Holland bulb company, is a bulb which can be normally opened after supercooling and storing treatment, is subjected to planting tests under different temperature changing treatments, and the stamen flap degree is identified, and the specific steps are as follows:
performing valve value assignment on stamens of all flowers in a red double happiness single plant;
the stamen valve value assignment method comprises the following steps: based on morphological description of different stamen valve values in the specification, valve value assignment is carried out on each stamen, the valve degree of each stamen is divided into 6 grades, and valve degree is assigned from low to high to 0-5;
step 5: identifying the degree of stamen valve:
calculating the average value of the stamen valve value of each lily as the stamen valve value of the lily; the mean value of the stamen of 6 plants under the same treatment condition is the stamen under the same condition; the calculation result is shown in fig. 4b;
according to the stamen valve value, identifying the stamen valve degree under different temperature changing treatment conditions, wherein the specific steps are as follows:
the stamen valve values of the treatment S3, the treatment S4 and the treatment S5 are all more than 4, so that the stamen valve degree is high, and the treatment has great commercial value;
the stamen valve value of the treatment S1 and the treatment S2 is less than 1, so that the stamen valve degree is low and the commercial value is low.
Finally, it should be noted that the above description is only for illustrating the technical solution of the present invention, and not for limiting the scope of the present invention, and that the simple modification and equivalent substitution of the technical solution of the present invention can be made by those skilled in the art without departing from the spirit and scope of the technical solution of the present invention.
Claims (11)
1. A method of controlling lily stamen valves, characterized by: treating lily by adjusting temperature, and then carrying out growth management on the lily until the lily flowers, and identifying the degree of stamen valvulation of the lily so as to control the stamen valvulation of the lily; the stamen petal of the lily can be controlled by temperature.
2. The method according to claim 1, characterized in that: the temperature is 14-22 ℃.
3. The method according to claim 1, characterized in that: the step of growth management comprises the treatment of illumination time and the application of water-soluble fertilizer.
4. A method according to claim 3, characterized in that: the illumination time is 10-16h.
5. The method according to claim 1, characterized in that: the identification method comprises the following steps: the average value of the stamen valvulation of the plants under the same planting condition is the stamen valvulation value under the condition, and the valvulation degree is judged according to the valvulation value, and the method specifically comprises the following steps:
the stamen valve value is more than or equal to 4, so that the stamen valve degree is high;
the stamen valve value is more than 1 and less than 4, and the stamen valve degree is moderate;
the stamen valve value is less than or equal to 1, and the stamen valve degree is low.
6. The method according to claim 1, characterized in that: the method comprises the following steps:
s1, preparing materials: preparing lily bulbs which can bloom normally after refrigeration treatment, wherein the diameter of the bulbs is 14-20cm, peat is used as a matrix, the specification of the matrix is 10-40mm, the thickness of the covering soil is 2 times of the height of the bulbs, the planting temperature is 14-28 ℃, and water-soluble fertilizer is applied after the soil surface is dried, and the bulbs are planted until top buds of the lily are planted;
s2, temperature treatment: transferring the lily with terminal bud soil into a planting environment with the temperature of 14-22 ℃, controlling the illumination intensity to 15000-80000Lx and the illumination duration to 10-16h, and applying water-soluble fertilizer after the soil surface is dried, and performing temperature treatment;
s3, growth management: transferring the lily subjected to the S2 treatment into a planting environment at 14-28 ℃, controlling the illumination intensity to 15000-80000Lx and the illumination duration to 10-16h, and applying a water-soluble fertilizer after the soil surface is dried until flowering;
s4, assigning a stamen valve value: performing valvulation value assignment on stamens of the lily after flowering;
s5, identifying the degree of stamen valve: the average value of the stamen valvulation of the plants under the same planting condition is the stamen valvulation value under the condition, and the valvulation degree is judged according to the valvulation value, and the method specifically comprises the following steps:
the stamen valve value is more than or equal to 4, so that the stamen valve degree is high;
the stamen valve value is more than 1 and less than 4, and the stamen valve degree is moderate;
the stamen valve value is less than or equal to 1, and the stamen valve degree is low.
7. The method according to claim 6, wherein: and (3) the height of the terminal buds is not more than 2 times of that of lily bulbs when the terminal buds are unearthed in the step (S1).
8. The method according to claim 6, wherein: and step S2, the time of the temperature treatment is from the time when the terminal buds are unearthed to the time when the minimum flower buds grow to 3 mm.
9. The method according to claim 6, wherein: the assignment method in step S4 is as follows: the degree of valvulation of the stamen was classified into 6 grades, and the degree of valvulation was assigned 0 to 5 from low to high, respectively.
10. The method according to claim 9, wherein: the 6 grades are specifically: level 0, 4 stamen chambers, more pollen and filigree shape; 1 grade, 2 stamen chambers, less pollen and filigree shape; grade 2, malformation of stamen and medicine room, no pollen and filigree shape; grade 3, stamen drug chamber malformation, no pollen, basal petal of filigree; 4, the stamens have no medicine room and filament shape; grade 5, stamen without drug chamber and filament basal petal.
11. Use of the method of any one of claims 1-10 for controlling or improving lily stamen petal formation.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550245A (en) * | 2011-12-27 | 2012-07-11 | 江苏省农业科学院 | Method for cultivating early blossoming and multiplicate rhododendron |
| CN105367189A (en) * | 2015-11-28 | 2016-03-02 | 黄平县绿意花卉有限公司 | Liquid medicine for promoting lily stamen petalody |
| US20170258023A1 (en) * | 2014-11-25 | 2017-09-14 | Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. | Vernalization independent lisianthus plants |
| CN110741901A (en) * | 2019-11-13 | 2020-02-04 | 云南省农业科学院花卉研究所 | Cultivation method of heavy valve hippeastrum rutilum |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550245A (en) * | 2011-12-27 | 2012-07-11 | 江苏省农业科学院 | Method for cultivating early blossoming and multiplicate rhododendron |
| US20170258023A1 (en) * | 2014-11-25 | 2017-09-14 | Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. | Vernalization independent lisianthus plants |
| CN105367189A (en) * | 2015-11-28 | 2016-03-02 | 黄平县绿意花卉有限公司 | Liquid medicine for promoting lily stamen petalody |
| CN110741901A (en) * | 2019-11-13 | 2020-02-04 | 云南省农业科学院花卉研究所 | Cultivation method of heavy valve hippeastrum rutilum |
Non-Patent Citations (2)
| Title |
|---|
| 范天刚 等: "低温诱导切花月季过度重瓣化的形态学观察", 《东北林业大学学报》, vol. 42, no. 9, 30 September 2014 (2014-09-30), pages 116 - 121 * |
| 许林;陈法志;戢小梅;童俊;李卓;: "川鄂连蕊茶开花生物学习性研究", 种子, no. 09, 25 September 2011 (2011-09-25), pages 49 - 51 * |
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