CN116194140A - 冠状病毒疾病(covid-19)疫苗 - Google Patents

冠状病毒疾病(covid-19)疫苗 Download PDF

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CN116194140A
CN116194140A CN202180032144.3A CN202180032144A CN116194140A CN 116194140 A CN116194140 A CN 116194140A CN 202180032144 A CN202180032144 A CN 202180032144A CN 116194140 A CN116194140 A CN 116194140A
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M·J·施奈尔
C·维尔布里奇
D·库鲁普
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Thomas Jefferson University
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Abstract

本发明包括包含SARS‑CoV‑2刺突蛋白(S)或其部分的疫苗及其使用方法。

Description

冠状病毒疾病(COVID-19)疫苗
相关申请的交叉引用
本申请要求2020年3月6日提交的美国临时申请号62/986,396和2020年4月29日提交的美国临时申请号63/017,241的优先权，其每一篇都通过引用以其整体并入本文。
关于联邦资助的研究和开发的声明
本发明是在政府支持下在由美国国立卫生研究院授予的基金号1R21AI158044-01下完成的。政府对本发明享有一定的权利。
序列表的引用
根据37 C.F.R.§1.52(e)(5)，将作为创建于2021年3月4日的名为“205961_7055WO1_Sequence_Listing.txt”且大小为577,339字节的文本文件一起提交的序列表通过引用并入本文。
背景技术
最近出现的冠状病毒，目前称为2019-nCoV或SARS-CoV-2病毒迅速传播，截至2020年2月10日，已有超过42,000例病例、500例死亡并且病例在18个国家出现。这种新型冠状病毒出现大量人际传播。已经确定了几个分离株的序列，并且最近的菌株是类似SARS的蝙蝠冠状病毒谱系。人们对这种病毒知之甚少，包括它对抗病毒化合物的敏感性、在细胞系中复制的能力或调节复制的宿主因子。重要的是，尽管正在进行调查研究，但是没有可用于治疗该病毒的疗法。当前爆发的模型表明，该病毒可能感染超过10亿人，并成为每年一次的流行病。
需要产生用于治疗冠状病毒，特别是COVID-19的疫苗的新方法。本发明解决并满足了这种需要。
发明内容
在一些方面中，本公开内容提供了一种编码重组病毒的分离核酸，该重组病毒包括SARS-CoV-2刺突蛋白(S)或其部分。
在其他方面中，本公开内容提供了包括SARS-CoV-2刺突蛋白(S)或其部分的重组病毒。
在一方面中，本公开内容提供了由本文描述的核酸编码的重组病毒。在一些实施方式中，核酸编码包括SARS-CoV-2刺突蛋白(S)或其部分的重组病毒。
在另一方面中，本公开内容提供了重组融合蛋白，其包括(a)病毒的糖蛋白(G)或其部分，和(b)SARS-CoV-2刺突蛋白(S)或其部分。
在一些方面中，本公开内容提供了包括本文描述的核酸的载体。在一个实施方式中，核酸编码包括SARS-CoV-2刺突蛋白(S)或其部分的重组病毒。
在其他方面中，本公开内容提供了一种疫苗，该疫苗包括包含SARS-CoV-2刺突蛋白(S)或其部分的重组病毒，以及药学上可接受的载体。
在一方面中，本公开内容提供了在有需要的受试者中产生针对SARS-CoV-2病毒的免疫反应的方法，该方法包括向受试者施用有效量的本文所述的疫苗。在一些实施方式中，疫苗包括包含SARS-CoV-2刺突蛋白(S)或其部分的重组病毒，以及药学上可接受的载体。
在另一方面中，本公开内容提供了向受试者接种针对SARS-CoV-2病毒的疫苗的方法，该方法包括向受试者施用有效量的本文所述的疫苗。在一些实施方式中，疫苗包括包含SARS-CoV-2刺突蛋白(S)或其部分的重组病毒，以及药学上可接受的载体。
在其他方面中，本公开内容提供了在受试者中提供针对SARS-CoV-2病毒的免疫力的方法，该方法包括向受试者施用有效量的本文所述的疫苗。在一些实施方式中，疫苗包括包含SARS-CoV-2刺突蛋白(S)或其部分的重组病毒，以及药学上可接受的载体。
在一些方面中，本公开内容提供了在有需要的受试者中治疗和/或预防与SARS-CoV-2病毒相关的疾病或紊乱的方法，该方法包括向受试者施用有效量的本文所述的疫苗。在一些实施方式中，疫苗包括包含SARS-CoV-2刺突蛋白(S)或其部分的重组病毒，以及药学上可接受的载体。
附图说明
当结合附图阅读时，将更好地理解以下对本发明具体实施方式的详细描述。为了说明本发明，在附图中显示了示例性实施方式。然而，应当理解，本发明不限于附图中所示实施例的精确布置和手段。
图1：表达密码子优化的Covid-S1的VSV。该图谱显示了病毒序列，其包括用于产生重组病毒的质粒。序列显示在SEQ ID NO：1中，并且特征显示在图7中。序列和特征还显示在图17中(“VSV-COVID19-S1-VSVG”)。
图2：表达密码子优化的Covid-S1的RABV。该图谱显示了病毒序列，包括用于产生重组病毒的质粒。序列显示在SEQ ID NO：2中，并且特征显示在图8中。序列和特征还显示在图18中(“BNSP333-COVID19-S1-RVG”)。
图3：来自基因组第2位的表达密码子优化的Covid-S的MV。该图谱显示了病毒序列，包括用于产生重组病毒的质粒。序列显示在SEQ ID NO：3中，并且特征显示在图9中。序列和特征还显示在图21中(“MV-coWuhan-S第2位”)。
图4：来自基因组第3位的表达密码子优化的Covid-S的MV。该图谱显示了病毒序列，包括用于产生重组病毒的质粒。序列显示在SEQ ID NO：4中，并且特征显示在图10中。序列和特征还描绘在图20中(“MV Wu S在第3位”)。
图5：来自基因组第6位的表达密码子优化的Covid-S的MV。该图谱显示了病毒序列，包括用于产生重组病毒的质粒。序列显示在SEQ ID NO：5中，并且特征显示在图11中。序列和特征还描绘在图19中(“MV WuhanCoV S在第6位”)。
图6：表达嵌合S1-G融合蛋白的RABV(RABV-COV19-S1)和VSV(VSV-COV19-S1)的表征。蔗糖梯度纯化后纯化的病毒体的SDS-PAGE分析。字母表示VSV或RABV L、G、N、P和M蛋白的位置。左边的数字表示分子量标准的大小。标明了Covid-19 S1蛋白。对照显示VSV和RABV病毒体。
图7显示了图1中显示的图谱的特征。
图8显示了图2中显示的图谱的特征。
图9显示了图3中显示的图谱的特征。
图10显示了图4中显示的图谱的特征。
图11显示了图5中显示的图谱的特征。
图12：本研究中使用的2019-nCoV疫苗构建体的示意图。刺突蛋白cDNA插入SAD-B19衍生的RABV病毒疫苗载体BNSP333的N和P基因之间。BNSP333-S1-G构建体表达嵌合蛋白，该蛋白包含与RABV G糖蛋白的C-末端部分(氨基酸428-524)融合的整个S1结构域，其中包括整个胞质结构域(CD)、跨膜结构域(TM)，外加RABV G胞外结构域(E31)的31个氨基酸。刺突蛋白的不同结构元件在全长构建体中显示：信号肽(SP)、受体结合域(RBD)、融合肽(FP)、七肽重复区1和2(HR1和HR2)、跨膜结构域(TM)和胞质结构域(CD)。
图13：MERSRAB免疫计划和MERS-CoV攻击的示意图。用灭活疫苗分析了两次和三次免疫。
图14A-14C：接种2次或3次MERSRAB可使动物免于体重减轻，并且疫苗诱导的免疫反应可将MERS-CoV攻击病毒控制到无法检测到的水平。如图13中概述的，对动物进行免疫和攻击。
图15：接种2次或3次MERSRAB可使动物免于肺部病毒复制。该图显示，在攻击后第3天，免疫小鼠肺部仅检测到少量MERS-CoV抗原，并且在第6天未检测到抗原。比较而言，在用狂犬病病毒载体免疫的动物的肺组织中，在与图14所示的病毒载量一致的两个时间点都仅检测到大量的组织损伤和病毒抗原。
图16A-16B：图16A是显示BBV151-A疫苗(BBV151-A1与BBV151-A2)制剂的示意图，和图16B是显示BBV-151-B疫苗制剂的示意图。
图17显示“VSV-COVID19-S1-VSVG”的序列和特征。
图18显示“BNSP333-COVID19-S1-RVG”的序列和特征。
图19显示“MV WuhanCoV S在第6位”的序列和特征。
图20显示“MV Wu S第3位”的序列和特征。
图21显示“MV-coWuhan-S第2位”的序列和特征。
具体实施方式
定义
除非另有定义，否则本文使用的所有技术和科学术语具有与本发明所属领域的普通技术人员通常理解的相同含义。尽管与本文所述的那些相似或等效的任何方法和材料可用于实践来测试本发明，但本文描述了优选的材料和方法。在描述和要求保护本发明时，将使用以下术语。
还应理解，本文使用的术语仅用于描述具体实施方式的目的，而不是限制性的。
如本文所使用的，冠词“一(a)”和“一(an)”用于指代一个或多于一个(即，至少一个)冠词的语法对象。举例来说，“一个元件”是指一个元件或多于一个元件。
如本文所使用的，术语“抗体(antibody)”或“Ab”是指衍生自免疫球蛋白分子的蛋白质或多肽序列，其特异性结合抗原上的特定表位。抗体可以是源自天然来源或重组来源的完整免疫球蛋白，并且可以是完整免疫球蛋白的免疫反应部分。可用于本发明的抗体可以多种形式存在，包括例如多克隆抗体、单克隆抗体、细胞内抗体(“胞内抗体(intrabody)”)、Fv、Fab和F(ab)2，以及单链抗体(scFv)和人源化抗体(Harlow等人，1998，Using Antibodies:A Laboratory Manual,Cold Spring Harbor Laboratory Press,NY；Harlow等人，1989，Antibodies:A Laboratory Manual,Cold Spring Harbor,New York；Houston等人，1988，Proc.Natl.Acad.Sci.USA 85:5879-5883；Bird等人，1988，Science242:423-426)。抗体可以来源于天然来源或重组来源。抗体通常是免疫球蛋白分子的四聚体。
术语“改善(ameliorating)”或“治疗(treating)”是指与疾病相关的临床体征和/或症状由于所采取的行动而减轻。待监测的体征或症状对于熟练的临床医生来说是众所周知的。
如本文所使用的，当提及可测量值比如量、持续时间等时，术语“约(about)”旨在涵盖与规定值相差±20％或±10％、更优选地±5％、甚至更优选地±1％和更优选地±0.1％的变量，因为这样的变化适合于执行所公开的方法。
术语“生物(biological)”或“生物样品(biological sample)”是指从生物体或从生物体的成分(例如，细胞)获得的样品。样品可以是任何生物组织或流体。样品通常是“临床样品”，它是来自患者的样品。这种样品包括但不限于骨髓、心脏组织、痰液、血液、淋巴液、血细胞(例如白细胞)、组织或细针活检样品、尿液、腹膜液和胸膜液或来自其的细胞。生物样品还可以包括组织切片，比如用于组织学目的的冷冻切片。
如本文所使用的，术语“对照(control)”或“参照(reference)”可互换使用并且指代用作比较标准的值。
如本文所使用的，术语“免疫原性(immunogenicity)”是指当将抗原或生物体施用于动物时抗原或生物体在动物中引发免疫反应的先天能力。因此，“增强免疫原性(enhancing the immunogenicity)”是指当将抗原或生物体施用于动物时增加抗原或生物体在动物中引发免疫反应的能力。可以通过以下方式测量增加的抗原或生物体引发免疫反应的能力：与抗原或生物体结合的更多的抗体、与抗原或生物体结合的抗体的更高的多样性、对抗原或生物体具有特异性的更多的T细胞、对抗原或生物体的更强的细胞毒性或辅助T细胞反应、对抗原反应的细胞因子的更高表达等。
如本文所使用的，术语“引发免疫反应(eliciting an immune response)”或“免疫(immunizing)”是指产生针对异源蛋白质的B细胞和/或T细胞反应的过程。
如本文所使用的，术语“抗原(antigen)”或“Ag”被定义为引发免疫反应的分子。这种免疫反应可能涉及抗体的产生，或特定免疫活性细胞的激活，或两者。熟练的技术人员将理解包括几乎所有的蛋白质或肽的任何大分子都可以用作抗原。此外，抗原可以来源于重组或基因组DNA。本领域技术人员将理解，包括编码引发免疫反应的蛋白质的核苷酸序列或部分核苷酸序列的任何DNA因此编码如本文所使用的术语“抗原”。此外，本领域技术人员将理解抗原不需要仅由基因的全长核苷酸序列编码。很明显，本发明包括但不限于使用多于一种基因的部分核苷酸序列，并且这些核苷酸序列以各种组合排列以引发所需的免疫反应。此外，技术人员将理解抗原根本不需要由“基因”编码。很明显，抗原可以合成产生或衍生自生物样品。这样的生物样品可以包括但不限于组织样品、肿瘤样品、细胞或生物流体。
本文使用的“异源抗原(heterologous antigens)”是指对包括或表达抗原的生物体而言不是内源的抗原。例如，包括或表达病毒或肿瘤抗原的病毒疫苗载体包括异源抗原。如本文所使用的，术语“异源蛋白质(heterologous protein)”是指在受试者(即哺乳动物)中引发有益免疫反应的蛋白质，无论其来源如何。
术语“特异性结合(specifically binds)”、“选择性结合(selectively bind)”或“结合特异性(binding specificity)”是指本发明的人源化抗体或结合化合物以比当与非靶标表位结合时产生的亲和力更高的亲和力与靶标表位结合的能力。在某些实施方式中，特异性结合是指以高于与非靶表位的亲和力至少10、50、100、250、500或1000倍的亲和力结合靶标。
如本文所使用的，“组合疗法(combination therapy)”是指第一种药剂与另一种药剂联合施用。“组合(in combination with)”或“结合(in conjunction with)”是指除了另一种治疗方式之外还施用一种治疗方式。因此，“组合(in combination with)”是指在向个体递送另一种治疗方式之前、期间或之后施用一种治疗方式。这种组合被认为是单一治疗方案或方案的一部分。
“体液免疫(humoral immunity)”或“体液免疫反应(humoral immune response)”二者均指B细胞介导的免疫，并且由B淋巴细胞(B细胞)产生和分泌的高度特异性抗体介导。
“预防(prevention)”是指使用药物组合物来针对疾病进行疫苗接种。
“佐剂(adjuvant)”是指能够增强抗原免疫原性的物质。佐剂可以是一种物质或多种物质的混合物，并且通过直接作用于免疫系统或通过提供抗原的缓慢释放而发挥作用。佐剂的实例包括但不限于乳剂(例如，水包油(o/w)乳剂)、铝盐、聚阴离子、细菌糖肽和缓释剂比如弗氏不完全。
“递送载体(delivery vehicle)”是指有助于将抗原靶向特定细胞并促进免疫系统有效识别抗原的组合物。最熟知的递送载体是脂质体、病毒体、包括微球和纳米球的微粒、聚合物、细菌菌影(bacterial ghost)、细菌多糖、减毒细菌、病毒样颗粒、减毒病毒和ISCOMS。
如本文所使用的，术语“表达(expression)”定义为由其启动子驱动的特定核苷酸序列的转录和/或翻译。
如本文所使用的，术语“表达盒(expression cassette)”是指能够指导异源编码序列的转录和/或翻译的核酸序列。在一些实施方式中，表达盒包含与编码异源蛋白质的序列可操作地连接的启动子序列。在一些实施方式中，表达盒进一步包括与编码异源蛋白质的序列可操作地连接的至少一个调节序列。
“并入……中(incorporated into)”或“包封在……中(encapsulated in)”是指在递送载体，比如微粒、细菌菌影、减毒细菌、病毒样颗粒、减毒病毒、ISCOM、脂质体和优选地病毒体内的抗原肽和/或核酸分子。
如本文所使用的，术语“肽(peptide)”、“多肽(polypeptide)”和“蛋白质(protein)”可互换使用，并且是指由通过肽键共价连接的氨基酸残基组成的化合物。蛋白质或肽必须包含至少两个氨基酸，并且对可能构成蛋白质或肽序列的氨基酸的最大数量没有限制。多肽包括包含通过肽键彼此连接的两个或更多个氨基酸的任何肽或蛋白质。如本文所使用的，该术语指代短链和长链，短链在本领域中也通常称为例如肽、寡肽和寡聚体，长链在本领域中通常称为蛋白质，其中有很多类型。“多肽(polypeptide)”包括例如生物活性片段、基本上同源的多肽、寡肽、同源二聚体、异源二聚体、多肽的变体、修饰的多肽、衍生物、类似物、融合蛋白等。多肽包括天然肽、重组肽、合成肽或它们的组合。
如本文所使用的，“融合蛋白(fusion protein)”是指一种蛋白质，其中该蛋白质包括通过肽键或其他化学键连接在一起的两种或更多种蛋白质。蛋白质可以通过肽或其他化学键直接连接在一起，或者通过两个或多个蛋白质之间的一个或多个氨基酸(在本文中称为间隔区)连接在一起。
在本发明的上下文中，使用以下常用核酸碱基的缩写。“A”是指腺苷，“C”是指胞嘧啶，“G”是指鸟苷，“T”是指胸苷，和“U”是指尿苷。
如本文所使用的，术语“RNA”定义为核糖核酸。
“转化(Transform)”、“转化(transforming)”和“转化(transformation)”在本文中用于指将分离核酸引入生物体内部的过程。
如在本发明的上下文中使用的术语“治疗(treatment)”意在包括疾病或紊乱的治疗性治疗以及预防性或抑制性措施。如本文所使用的，术语“治疗(treatment)”和相关术语例如“治疗(treat)”和“治疗(treating)”是指疾病状况或其至少一种症状的进展、严重性和/或持续时间的降低。因此，术语“治疗(treatment)”是指可以使受试者受益的任何方案。治疗可以针对现有病症或可以是预防性的(预防性治疗)。治疗可能包括治疗、缓解或预防效果。本文提及的“治疗性(therapeutic)”和“预防性(prophylactic)”治疗应在其最广泛的范围内考虑。术语“治疗性(therapeutic)”并不一定意味着对受试者进行治疗直到完全康复。类似地，“预防性(prophylactic)”并不一定意味着受试者最终不会感染疾病病症。因此，例如，术语治疗包括在疾病或病症发作之前或之后施用药剂，从而预防或消除疾病或紊乱的所有病症。作为另一个实例，在疾病的临床表现之后施用药剂以对抗疾病的症状包括疾病的“治疗(treatment)”。
术语“等同的(equivalent)”在用于提及核苷酸序列时，应理解为是指编码功能等价多肽的核苷酸序列。等同的核苷酸序列将包括通过一个或多个核苷酸取代、添加或缺失而不同的序列，比如等位基因变体；并且因此，将包括由于遗传密码的简并性而不同于本文所述核酸的核苷酸序列的序列。
如本文所使用的关于核酸比如DNA或RNA的术语“分离的(isolated)”是指分别与存在于天然来源的大分子中的其他DNA或RNA分离的分子。如本文所使用的术语，分离还指当通过重组DNA技术产生时基本上不含细胞材料、病毒材料或培养基，或在化学合成时基本上不含化学前体或其他化学物质的核酸或肽。此外，“分离核酸(isolated nucleic acid)”意在包括不天然存在为片段并且不会在天然状态下被发现的核酸片段。术语“分离的(isolated)”在本文中也用于指从其他细胞蛋白中分离的多肽，并且意在包括纯化的和重组的多肽。“分离细胞(isolated cell)”或“分离细胞群(isolated population of cell)”是不存在于其天然环境中的细胞或细胞群。
如本文所使用的，“同一性(identity)”是指两个聚合分子之间，特别是两个氨基酸分子之间，例如两个多肽分子之间的亚基序列同一性。当两个氨基酸序列在相同位置具有相同残基时；例如，如果两个多肽分子中的每一个的某位置被一个精氨酸占据，那么它们在那个位置上是相同的。在比对中，两个氨基酸序列在相同位置具有相同残基的同一性或程度通常以百分比表示。两个氨基酸序列之间的同一性是匹配或相同位置数量的直接函数；例如，如果两个序列中的一半位置(例如，长度为十个氨基酸的聚合物中的五个位置)相同，则两个序列有50％相同；如果90％的位置(例如，10个中的9个)匹配或相同，则两个氨基酸序列是90％相同的。
其中使用的“突变(mutation)”是产生其天然状态改变的DNA序列的变化。突变可以包括至少一种脱氧核糖核酸碱基比如嘌呤(腺嘌呤和/或胸腺嘧啶)和/或嘧啶(鸟嘌呤和/或胞嘧啶)的缺失和/或插入和/或重复和/或取代。
突变可能会或可能不会对生物体的可观察特征(表型)产生可辨别的变化。
如本文所使用的，术语“核酸(nucleic acid)”是指多核苷酸，例如脱氧核糖核酸(DNA)，并且在适当的情况下指核糖核酸(RNA)。该术语还应理解为包括由核苷酸类似物制成的RNA或DNA的类似物以及当适用于所描述的实施方式时的单链(有义或反义)和双链多核苷酸作为等价物。EST、染色体、cDNA、mRNA和rRNA是可称为核酸的分子的代表性实例。如本文所使用的，核酸包括但不限于通过本领域可用的任何方式，包括但不限于重组方式，即从重组文库中克隆核酸序列或病毒基因组，使用普通克隆技术和PCRTM等，以及通过合成方式获得的所有核酸序列。
在本发明的上下文中，使用以下常用核酸碱基的缩写。“A”是指腺苷，“C”是指胞嘧啶，“G”是指鸟苷，“T”是指胸苷，和“U”是指尿苷。
如本文所使用的，“可操作地连接的(operably linked)”序列包括与目的基因邻接的表达控制序列和反式或远距离作用以控制目的基因的表达控制序列。表达控制序列包括适当的转录起始、终止、启动子和增强子序列；高效的RNA处理信号，比如剪接和多腺苷酸化(polyA)信号；稳定细胞质mRNA的序列；提高翻译效率的序列(即，Kozak共有序列)；增强蛋白质稳定性的序列；并且当需要时，增强编码产物分泌的序列。有许多表达控制序列，包括本领域已知的可用于本发明组合物的天然、组成型、诱导型和/或组织特异性的启动子。“可操作地连接”应解释为除了DNA表达和控制序列之外还包括RNA表达和控制序列。
如本文所使用的，术语“启动子(promoter)”定义为启动多核苷酸序列的特异性转录所需的由细胞的合成机制或引入的合成机制识别的DNA序列。
如本文所使用的，术语“启动子/调控序列(promoter/regulatory sequence)”是指对于表达与启动子/调控序列可操作连接的基因产物所必需的核酸序列。在一些情况下，该序列可以是核心启动子序列，而在其他情况下，该序列还可以包括基因产物表达所需的增强子序列和其他调节元件。例如，启动子/调节序列可以是以组织特异性方式表达基因产物的序列。
“组成型(constitutive)”启动子是当它与编码或指定基因产物的多核苷酸可操作地连接时，使得在细胞的大部分或所有生理条件下在细胞中产生基因产物的核苷酸序列。
“诱导型(inducible)”启动子是当它与编码或指定基因产物的多核苷酸可操作地连接时，基本上仅当对应于启动子的诱导物存在于细胞中时，才会使基因产物在细胞中产生的核苷酸序列。
如本文所使用的，术语“药物组合物(pharmaceutical composition)”是指在本发明中至少一种有用的化合物与其他化学组分的混合物，其他化学组分比如载体、稳定剂、稀释剂、佐剂、分散剂、悬浮剂、增稠剂和/或赋形剂。药物组合物有助于将化合物施用于生物体。本领域存在多种施用化合物的技术，包括但不限于：静脉内、口服、喷雾、肠胃外、眼部、肺部和局部施用。
术语“药学上可接受的载体(pharmaceutically acceptable carrier)”包括在受试者内或向受试者提供参与携带或运输本发明的化合物(一种或多种)以使其可以执行其预期的功能的药学上可接受的盐、药学上可接受的材料、组合物或载体，比如液体或固体填充剂、稀释剂、赋形剂、溶剂或包封材料。通常，这种化合物从一个器官或身体的一部分被携带或运输到另一器官或身体的一部分。在与制剂的其他成分相容的意义上，每种盐或载体必须是“可接受的(acceptable)”，并且对受试者无害。可用作药学上可接受的载体的材料的一些实例包括：糖，比如乳糖、葡萄糖和蔗糖；淀粉，比如玉米淀粉和马铃薯淀粉；纤维素及其衍生物，比如羧甲基纤维素钠、乙基纤维素和醋酸纤维素；粉状黄蓍胶；麦芽；明胶；滑石；赋形剂，比如可可脂和栓剂蜡；油，比如花生油、棉籽油、红花油、芝麻油、橄榄油、玉米油和大豆油；二醇，比如丙二醇；多元醇，比如甘油、山梨糖醇、甘露醇和聚乙二醇；酯，比如油酸乙酯和月桂酸乙酯；琼脂；缓冲剂，比如氢氧化镁和氢氧化铝；藻酸；无热原水；等渗盐水；林格氏溶液；乙醇；磷酸盐缓冲溶液；稀释剂；成粒剂；润滑剂；粘结剂；崩解剂；润湿剂；乳化剂；着色剂；脱模剂；涂层剂；甜味剂；调味剂；加香剂；防腐剂；抗氧化剂；增塑剂；凝胶剂；增稠剂；硬化剂；定型剂；悬浮剂；表面活性剂；保湿剂；载体；稳定剂；和其他在药物制剂中使用的无毒相容物质，或它们的任何组合。如本文所使用的，“药学上可接受的载体”还包括与化合物的活性相容并且受试者生理学上可接受的任何和所有涂层、抗菌剂和抗真菌剂以及吸收延迟剂等。补充的活性化合物也可以并入组合物中。
如本文所使用的，术语“有效量(effective amount)”或“治疗有效量(therapeutically effective amount)”是指实现预期结果，例如，产生预期的免疫学、药理学、治疗和/或保护结果所必需的组合物(例如疫苗组合物)或活性成分(例如病毒样颗粒(VLP)、病毒体、病毒载体、抗原、核酸分子)的量(例如，足以诱导可测量的免疫反应、预防特定疾病病症、降低疾病病症或与之相关的至少一种症状和/或病症的严重程度和/或对其改善的VLP、病毒体或病毒载体的量)。
如本文所使用的，“受试者(subject)”或“患者(patient)”可以是人类或非人类哺乳动物。非人类哺乳动物包括例如家畜和宠物，比如羊、牛、猪、犬、猫和鼠哺乳动物。优选地，受试者是人。在一些实施方式中，受试者是家养宠物或家畜。在一些实施方式中，受试者是猫。在一些实施方式中，受试者是狗。在一些其他实施方式中，受试者是雪貂。
“滴度(titer)”是与参考样品相比病毒或病毒载体浓度的数值测量值，其中浓度由病毒的活性或通过测量单位体积缓冲液中的病毒数量来确定。例如，通过使用软琼脂方法测量病毒的一种或多种溶液(通常连续稀释)对HeLa细胞的感染性(参见Graham&Van Dereb(1973)Virology 52:456-467)或通过监测赋予细胞的抗性(例如，由病毒或载体编码的G418抗性)或通过紫外分光光度法对病毒进行定量(参见Chardonnet&Dales(1970)Virology40:462-477)来确定病毒原液的滴度。
“疫苗接种(vaccination)”是指给受试者接种抗原以在受试者中引发免疫反应的过程，这有助于预防或治疗与抗原相关的疾病或紊乱。术语“免疫(immunization)”在本文中可与疫苗接种互换使用。
“载体(vector)”是包含核酸并且可用于将核酸递送至细胞内部的物质组合物。许多载体是本领域已知的，包括但不限于线性多核苷酸、与离子或两亲化合物相关的多核苷酸、质粒和病毒。在本公开内容中，术语“载体”包括自主复制病毒。
范围：在整篇公开内容中，本发明的各个方面可以以范围格式呈现。应当理解，范围格式的描述仅仅是为了方便和简洁，而不应理解为对本发明范围的不灵活限制。因此，范围的描述应该被认为已经具体公开了所有可能的子范围以及该范围内的各个数值。例如，对范围诸如1到6的描述应该被认为具体公开了子范围，比如1到3、1到4、1到5、2到4、2到6、3到6等，以及该范围内的单个数字，例如1、2、2.7、3、4、5、5.3和6。无论范围的广度如何，这都适用。
描述
本发明涉及用于产生针对SARS-CoV-2病毒的疫苗的组合物和方法。SARS-CoV-2病毒在本文中也称为2019-nCoV或2019新型冠状病毒。
本文描述了一种针对SARS-CoV-2病毒的疫苗，该疫苗使用基于狂犬病病毒的载体，该载体已被证明是针对新出现和再次出现的传染病的有效载体。先前已经证明，含有MERS-CoV刺突S1蛋白的灭活狂犬病毒颗粒可诱导针对MERS-CoV和RABV的有效免疫反应，并在动物系统中提供保护。
本文所述的2019-nCoV疫苗具有以下优点：
·该构建体基于目前可用的狂犬病疫苗产品，并且因此将有助于疫苗进入临床一期研究，以便在短时间内准备好。Bharat Biotech Ltd(BBIL)目前每年生产2000万剂RABV疫苗。
·该构建体可以根据需要快速扩大规模，以进行额外的临床试验和商业生产。
·该构建体可以以低成本制造。狂犬病疫苗已经商业化生产了几十年，成本众所周知并且价格低廉。本疫苗使用与目前人类狂犬病疫苗相同的制造工艺。在资源有限的地区为数百万人接种疫苗时，低成本疫苗是一个显著优势。
·该疫苗对所有人群都应该是安全的。在一些实施方式中，疫苗基于灭活狂犬病疫苗骨架，因为狂犬病疫苗在不同人群中具有数十年的安全使用。
·已基于在两个小鼠模型和羊驼(骆驼科)中使用相关MERS-CoV进行的攻击研究，通过在动物模型中的保护证实了概念证明。
·由于RABV疫苗通常提供终生保护，因此有望获得长期保护。
·该疫苗可以由经验丰富的商业规模制造合作伙伴Bharat Biotech生产，该合作伙伴已成功符合世卫组织对其他疫苗的资格预审标准，并可迅速进入临床试验和商业生产。Bharat Biotech符合FDA、EMA和WHO PQ的cGMP标准。
·使用一种独特的多糖佐剂Advax-SM，该佐剂已在人体临床试验中进行了广泛测试，显示可增强体液免疫和细胞免疫。最值得注意的是，将Advax-SM添加到原型cGMP全细胞和重组刺突蛋白SARS疫苗中不仅增强了中和抗体反应并阻止了肺病毒复制，而且还完全防止了疫苗增强的嗜酸性肺病理学对SARS病毒暴露的反应。
·RABV载体和使用的佐剂都可以在室温下稳定和储存。
构建体
在一方面中，本发明包括一种编码重组病毒的分离核酸，该重组病毒包括SARS-CoV-2刺突蛋白(S)或其部分。在一些实施方式中，病毒是弹状病毒。在一些实施方式中，病毒是狂犬病病毒、水疱性口炎病毒(VSV)或麻疹病毒。在具体实施方式中，病毒是狂犬病病毒。核酸可以包括为在细胞(例如，哺乳动物细胞、人类细胞)中表达而密码子优化的序列。
在一个实施方式中，SARS-CoV-2刺突蛋白(S)或其部分包括如SEQ ID NO：28中所示的氨基酸序列：
MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPR。
在另一个实施方式中，SARS-CoV-2刺突蛋白(S)或其部分包括如SEQ ID NO：29中所示的氨基酸序列：
MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT。
在一个实施方式中，SARS-CoV-2刺突蛋白(S)或其部分包括如SEQ ID NO：30中所示的氨基酸序列：
MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRSVGDEAEDFVEVHLPDVHNQVSGVDLGLPNWGKYVLLSAGALTALMLIIFLMTCCRRVNRSEPTQHNLRGTGREVSVTPQSGKIISSWESHKSGGETRL。
在另一个实施方式中，SARS-CoV-2刺突蛋白(S)或其部分包括如在SEQ ID NO：31中所示的氨基酸序列：
MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPR。
在另一个实施方式中，提供了一种N蛋白质(N)或其部分，包括如SEQ ID NO：32中所示的氨基酸序列：
MATLLRSLALFKRNKDKPPITSGSGGAIRGIKHIIIVPIPGDSSITTRSRLLDRLVRLIGNPDVSGPKLTGALIGILSLFVESPGQLIQRITDDPDVSIRLLEVVQSDQSQSGLTFASRGTNMEDEADQYFSHDDPISSDQSRFGWFGNKEISDIEVQDPEGFNMILGTILAQIWVLLAKAVTAPDTAADSELRRWIKYTQQRRVVGEFRLERKWLDVVRNRIAEDLSLRRFMVALILDIKRTPGNKPRIAEMICDIDTYIVEAGLASFILTIKFGIETMYPALGLHEFAGELSTLESLMNLYQQMGETAPYMVILENSIQNKFSAGSYPLLWSYAMGVGVELENSMGGLNFGRSYFDPAYFRLGQEMVRRSAGKVSSTLASELGITAEDARLVSEIAMHTTEDKISRAVGPRQAQVSFLHGDQSENELPRLGGKEDRRVKQSRGEARESYRETGPSRASDARAAHLPTGTPLDIDTATESSQDPQDSRRSADALLRLQAMAGISEEQGSDTDTPIVYNDRNLLD。
在另一个实施方式中，SARS-CoV-2刺突蛋白(S)或其部分是变体刺突多肽，其包括相对于如SEQ ID NO：28、29、30或31中所示的氨基酸序列，在其氨基酸序列中的一种或多种改变(例如，取代(一个或多个)、插入(一个或多个)、缺失(一个或多个)、添加(一个或多个)、修饰(一个或多个))。
在其他实施方式中，SARS-CoV-2刺突蛋白(S)或其部分是变体刺突多肽，其包括相对于如SEQ ID NO：28、29、30或31中所示的氨基酸序列，至少1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30或更多个变化。
在一个实施方式中，SARS-CoV-2刺突蛋白(S)或其部分是变体刺突多肽，其包括与如SEQ ID NO：28、29、30或31中所示的氨基酸序列至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％，但不是100％的序列同一性。
在另一个实施方式中，N蛋白质(N)或其部分为变体N多肽，其包括相对于如SEQ IDNO：32中所示的氨基酸序列，在其氨基酸序列中的一个或多个变化(例如，取代(一个或多个)、插入(一个或多个)、缺失(一个或多个)、添加(一个或多个)、修饰(一个或多个))。
在其他实施方式中，N蛋白质(N)或其部分为变体N多肽，其包括相对于如SEQ IDNO：32中所示的氨基酸序列，至少1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30或更多个变化。
在一个实施方式中，N蛋白质(N)或其部分为变体N多肽，其包括与如SEQ ID NO：32中所示的氨基酸序列至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％，但不是100％的序列同一性。
在一些实施方式中，重组病毒表达全长SARS-CoV-2刺突蛋白(S)。在一些其他实施方式中，重组病毒表达SARS-CoV-2刺突蛋白(S)的一部分。在一个实施方式中，SARS-CoV-2刺突蛋白(S)的部分是SARS-CoV-2刺突蛋白(S)的受体结合位点。在另一个实施方式中，SARS-CoV-2刺突蛋白(S)的部分是S1结构域。在另一个实施方式中，SARS-CoV-2刺突蛋白(S)的部分是SARS-CoV-2刺突蛋白(S)的N-末端750个氨基酸。
在一些实施方式中，SARS-CoV-2刺突蛋白(S)或其部分与糖蛋白(G)或其部分融合。在一些实施方式中，糖蛋白(G)包含在第333位用谷氨酸取代精氨酸的突变。在一些实施方式中，糖蛋白(G)的部分包含胞外结构域、胞质结构域和跨膜结构域。在其他实施方式中，糖蛋白(G)的部分包括胞外结构域的1至约100个氨基酸或三聚结构域。在一些实施方式中，糖蛋白(G)的部分包含胞外结构域的31个氨基酸。在一些实施方式中，糖蛋白(G)包含胞外结构域的31个氨基酸和全长胞质结构域。
在一些实施方式中，编码SARS-CoV-2刺突蛋白(S)或其部分的序列被密码子优化以在细胞中表达。在一些实施方式中，编码与糖蛋白(G)或其部分融合的SARS-CoV-2刺突蛋白(S)或其部分的序列被密码子优化以在细胞中表达。
在一些实施方式中，核酸包括编码病毒基因组的至少一部分的序列。在一些实施方式中，分离核酸包括全长基因组。
在一个实施方式中，核酸包括(a)编码水疱性口炎病毒(VSV)基因组的至少一部分的序列，和(b)编码包括SARS-CoV-2刺突蛋白(S)或其部分的蛋白质的序列。在一些实施方式中，包括SARS-CoV-2刺突蛋白(S)或其部分的蛋白质是包括糖蛋白(G)或其部分和SARS-CoV-2刺突蛋白(S)或其部分的融合蛋白。在一些实施方式中，融合蛋白包括与SARS-CoV-2刺突蛋白(S)的S1结构域融合的糖蛋白(G)或其部分。
在另一个实施方式中，核酸包括SEQ ID NO：1的序列，如下所示：
CTGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAGcgcgccCTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACCCACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTAGGGTCTTCGTCTGATGAGTCCGTGAGGACGAAACCCGGCGTACCGGGTCACGAAGACAAACAAACCATTATTATCATTAAAAGGCTCAGGAGAAACTTTAACAGTAATCAAAATGTCTGTTACAGTCAAGAGAATCATTGACAACACAGTCATAGTTCCAAAACTTCCTGCAAATGAGGATCCAGTGGAATACCCGGCAGATTACTTCAGAAAATCAAAGGAGATTCCTCTTTACATCAATACTACAAAAAGTTTGTCAGATCTAAGAGGATATGTCTACCAAGGCCTCAAATCCGGAAATGTATCAATCATACATGTCAACAGCTACTTGTATGGAGCATTAAAGGACATCCGGGGTAAGTTGGATAAAGATTGGTCAAGTTTCGGAATAAACATCGGGAAAGCAGGGGATACAATCGGAATATTTGACCTTGTATCCTTGAAAGCCCTGGACGGCGTACTTCCAGATGGAGTATCGGATGCTTCCAGAACCAGCGCAGATGACAAATGGTTGCCTTTGTATCTACTTGGCTTATACAGAGTGGGCAGAACACAAATGCCTGAATACAGAAAAAAGCTCATGGATGGGCTGACAAATCAATGCAAAATGATCAATGAACAGTTTGAACCTCTTGTGCCAGAAGGTCGTGACATTTTTGATGTGTGGGGAAATGACAGTAATTACACAAAAATTGTCGCTGCAGTGGACATGTTCTTCCACATGTTCAAAAAACATGAATGTGCCTCGTTCAGATACGGAACTATTGTTTCCAGATTCAAAGATTGTGCTGCATTGGCAACATTTGGACACCTCTGCAAAATAACCGGAATGTCTACAGAAGATGTAACGACCTGGATCTTGAACCGAGAAGTTGCAGATGAAATGGTCCAAATGATGCTTCCAGGCCAAGAAATTGACAAGGCCGATTCATACATGCCTTATTTGATCGACTTTGGATTGTCTTCTAAGTCTCCATATTCTTCCGTCAAAAACCCTGCCTTCCACTTCTGGGGGCAATTGACAGCTCTTCTGCTCAGATCCACCAGAGCAAGGAATGCCCGACAGCCTGATGACATTGAGTATACATCTCTTACTACAGCAGGTTTGTTGTACGCTTATGCAGTAGGATCCTCTGCCGACTTGGCACAACAGTTTTGTGTTGGAGATAACAAATACACTCCAGATGATAGTACCGGAGGATTGACGACTAATGCACCGCCACAAGGCAGAGATGTGGTCGAATGGCTCGGATGGTTTGAAGATCAAAACAGAAAACCGACTCCTGATATGATGCAGTATGCGAAAAGAGCAGTCATGTCACTGCAAGGCCTAAGAGAGAAGACAATTGGCAAGTATGCTAAGTCAGAATTTGACAAATGACCCTATAATTCTCAGATCACCTATTATATATTATGCTACATATGAAAAAAACTAACAGATATCATGGATAATCTCACAAAAGTTCGTGAGTATCTCAAGTCCTATTCTCGTCTGGATCAGGCGGTAGGAGAGATAGATGAGATCGAAGCACAACGAGCTGAAAAGTCCAATTATGAGTTGTTCCAAGAGGATGGAGTGGAAGAGCATACTAAGCCCTCTTATTTTCAGGCAGCAGATGATTCTGACACAGAATCTGAACCAGAAATTGAAGACAATCAAGGTTTGTATGCACCAGATCCAGAAGCTGAGCAAGTTGAAGGCTTTATACAGGGGCCTTTAGATGACTATGCAGATGAGGAAGTGGATGTTGTATTTACTTCGGACTGGAAACAGCCTGAGCTTGAATCTGACGAGCATGGAAAGACCTTACGGTTGACATCGCCAGAGGGTTTAAGTGGAGAGCAGAAATCCCAGTGGCTTTCGACGATTAAAGCAGTCGTGCAAAGTGCCAAATACTGGAATCTGGCAGAGTGCACATTTGAAGCATCGGGAGAAGGGGTCATTATGAAGGAGCGCCAGATAACTCCGGATGTATATAAGGTCACTCCAGTGATGAACACACATCCGTCCCAATCAGAAGCAGTATCAGATGTTTGGTCTCTCTCAAAGACATCCATGACTTTCCAACCCAAGAAAGCAAGTCTTCAGCCTCTCACCATATCCTTGGATGAATTGTTCTCATCTAGAGGAGAGTTCATCTCTGTCGGAGGTGACGGACGAATGTCTCATAAAGAGGCCATCCTGCTCGGCCTGAGATACAAAAAGTTGTACAATCAGGCGAGAGTCAAATATTCTCTGTAGACTATGAAAAAAAGTAACAGATATCACGATCTAAGTGTTATCCCAATCCATTCATCATGAGTTCCTTAAAGAAGATTCTCGGTCTGAAGGGGAAAGGTAAGAAATCTAAGAAATTAGGGATCGCACCACCCCCTTATGAAGAGGACACTAGCATGGAGTATGCTCCGAGCGCTCCAATTGACAAATCCTATTTTGGAGTTGACGAGATGGACACCTATGATCCGAATCAATTAAGATATGAGAAATTCTTCTTTACAGTGAAAATGACGGTTAGATCTAATCGTCCGTTCAGAACATACTCAGATGTGGCAGCCGCTGTATCCCATTGGGATCACATGTACATCGGAATGGCAGGGAAACGTCCCTTCTACAAAATCTTGGCTTTTTTGGGTTCTTCTAATCTAAAGGCCACTCCAGCGGTATTGGCAGATCAAGGTCAACCAGAGTATCACACTCACTGCGAAGGCAGGGCTTATTTGCCACATAGGATGGGGAAGACCCCTCCCATGCTCAATGTACCAGAGCACTTCAGAAGACCATTCAATATAGGTCTTTACAAGGGAACGATTGAGCTCACAATGACCATCTACGATGATGAGTCACTGGAAGCAGCTCCTATGATCTGGGATCATTTCAATTCTTCCAAATTTTCTGATTTCAGAGAGAAGGCCTTAATGTTTGGCCTGATTGTCGAGAAAAAGGCATCTGGAGCGTGGGTCCTGGATTCTATCAGCCACTTCAAATGAGCTAGTCTAACTTCTAGCTTCTGAACAATCCCCGGTTTACTCAGTCTCTCCTAATTCCAGCCTCTCGAACAACTAATATCCTGTCTTTTCTATCCCTATGAAAAAAACTAACAGAGATCGATCTGTTTACGCGTCACTATGAAGTGCCTTTTGTACTTAGCCTTTTTATTCATTGGGGTGAATTGCAAGTTCACCATAGTTTTTCCACACAACCAAAAAGGAAACTGGAAAAATGTTCCTTCTAATTACCATTATTGCCCGTCAAGCTCAGATTTAAATTGGCATAATGACTTAATAGGCACAGCCATACAAGTCAAAATGCCCAAGAGTCACAAGGCTATTCAAGCAGACGGTTGGATGTGTCATGCTTCCAAATGGGTCACTACTTGTGATTTCCGCTGGTATGGACCGAAGTATATAACACAGTCCATCCGATCCTTCACTCCATCTGTAGAACAATGCAAGGAAAGCATTGAACAAACGAAACAAGGAACTTGGCTGAATCCAGGCTTCCCTCCTCAAAGTTGTGGATATGCAACTGTGACGGATGCCGAAGCAGTGATTGTCCAGGTGACTCCTCACCATGTGCTGGTTGATGAATACACAGGAGAATGGGTTGATTCACAGTTCATCAACGGAAAATGCAGCAATTACATATGCCCCACTGTCCATAACTCTACAACCTGGCATTCTGACTATAAGGTCAAAGGGCTATGTGATTCTAACCTCATTTCCATGGACATCACCTTCTTCTCAGAGGACGGAGAGCTATCATCCCTGGGAAAGGAGGGCACAGGGTTCAGAAGTAACTACTTTGCTTATGAAACTGGAGGCAAGGCCTGCAAAATGCAATACTGCAAGCATTGGGGAGTCAGACTCCCATCAGGTGTCTGGTTCGAGATGGCTGATAAGGATCTCTTTGCTGCAGCCAGATTCCCTGAATGCCCAGAAGGGTCAAGTATCTCTGCTCCATCTCAGACCTCAGTGGATGTAAGTCTAATTCAGGACGTTGAGAGGATCTTGGATTATTCCCTCTGCCAAGAAACCTGGAGCAAAATCAGAGCGGGTCTTCCAATCTCTCCAGTGGATCTCAGCTATCTTGCTCCTAAAAACCCAGGAACCGGTCCTGCTTTCACCATAATCAATGGTACCCTAAAATACTTTGAGACCAGATACATCAGAGTCGATATTGCTGCTCCAATCCTCTCAAGAATGGTCGGAATGATCAGTGGAACTACCACAGAAAGGGAACTGTGGGATGACTGGGCACCATATGAAGACGTGGAAATTGGACCCAATGGAGTTCTGAGGACCAGTTCAGGATATAAGTTTCCTTTATACATGATTGGACATGGTATGTTGGACTCCGATCTTCATCTTAGCTCAAAGGCTCAGGTGTTCGAACATCCTCACATTCAAGACGCTGCTTCGCAACTTCCTGATGATGAGAGTTTATTTTTTGGTGATACTGGGCTATCCAAAAATCCAATCGAGCTTGTAGAAGGTTGGTTCAGTAGTTGGAAAAGCTCTATTGCCTCTTTTTTCTTTATCATAGGGTTAATCATTGGACTATTCTTGGTTCTCCGAGTTGGTATCCATCTTTGCATTAAATTAAAGCACACCAAGAAAAGACAGATTTATACAGACATAGAGATGAACCGACTTGGAAAGTAACTCAAATCCTGCTAGGTATGAAAAAAACTAACaGATATCACGCTCGAGCGTACGCCACCATGTTCGTGTTTCTGGTGCTGCTGCCTCTGGTGAGCTCCCAGTGCGTGAACCTGACCACAAGGACCCAGCTGCCCCCTGCCTATACCAATTCCTTCACACGGGGCGTGTACTATCCCGACAAGGTGTTCCGGAGCAGCGTGCTGCACTCCACACAGGATCTGTTTCTGCCTTTCTTTTCTAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAATGGCACAAAGCGGTTCGACAATCCAGTGCTGCCCTTTAACGATGGCGTGTACTTCGCCTCCACCGAGAAGTCTAACATCATCAGAGGCTGGATCTTTGGCACCACACTGGACAGCAAGACACAGTCCCTGCTGATCGTGAACAATGCCACCAACGTGGTCATCAAGGTGTGCGAGTTCCAGTTTTGTAATGATCCATTCCTGGGCGTGTACTATCACAAGAACAATAAGTCTTGGATGGAGAGCGAGTTTCGCGTGTATTCCTCTGCCAACAATTGCACATTTGAGTACGTGTCCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAATTTCAAGAACCTGAGGGAGTTCGTGTTTAAGAATATCGATGGCTACTTCAAAATCTACTCCAAGCACACCCCAATCAACCTGGTGCGCGACCTGCCACAGGGCTTCTCTGCCCTGGAGCCACTGGTGGATCTGCCCATCGGCATCAACATCACCCGGTTTCAGACACTGCTGGCCCTGCACAGAAGCTACCTGACACCAGGCGACAGCTCCTCTGGATGGACCGCAGGAGCAGCAGCCTACTATGTGGGCTATCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAATGGCACCATCACAGACGCCGTGGATTGCGCCCTGGATCCCCTGTCTGAGACCAAGTGTACACTGAAGAGCTTTACCGTGGAGAAGGGCATCTATCAGACAAGCAATTTCAGGGTGCAGCCTACCGAGTCCATCGTGCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAAGATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGGAGGGCTTTAACTGTTATTTCCCTCTGCAGAGCTACGGCTTCCAGCCAACAAACGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACCTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGACTGACCGGAACAGGCGTGCTGACCGAGTCCAACAAGAAGTTCCTGCCTTTTCAGCAGTTCGGCAGGGACATCGCAGATACCACAGACGCCGTGCGCGACCCTCAGACCCTGGAGATCCTGGACATCACACCATGCTCCTTCGGCGGCGTGTCTGTGATCACACCAGGCACCAATACAAGCAACCAGGTGGCCGTGCTGTATCAGGACGTGAATTGTACCGAGGTGCCAGTGGCAATCCACGCAGATCAGCTGACCCCTACATGGCGGGTGTACTCTACCGGCAGCAACGTGTTCCAGACAAGAGCCGGATGCCTGATCGGAGCAGAGCACGTGAACAATAGCTATGAGTGCGACATCCCTATCGGCGCCGGCATCTGTGCCTCCTACCAGACCCAGACAAACTCCCCAAGGTCTGTGGGCGATACAGGCCTGTCCAAGAATCCAATCGAGCTGGTAGAGGGCTGGTTCAGCAGTTGGAAAAGCTCCATCGCCTCCTTTTTCTTTATCATCGGCCTGATCATCGGACTGTTCCTGGTGCTCCGCGTGGGTATCCACCTGTGCATCAAGCTGAAGCACACCAAGAAAAGACAGATTTATACAGACATCGAGATGAACCGCCTGGGAAAGTGAGCTAGCCAGATTCTTCATGTTTGGACCAAATCAACTTGTGATACCATGCTCAAAGAGGCCTCAATTATATTTGAGTTTTTAATTTTTATGAAAAAAACTAACAGCAATCATGGAAGTCCACGATTTTGAGACCGACGAGTTCAATGATTTCAATGAAGATGACTATGCCACAAGAGAATTCCTGAATCCCGATGAGCGCATGACGTACTTGAATCATGCTGATTACAATTTGAATTCTCCTCTAATTAGTGATGATATTGACAATTTGATCAGGAAATTCAATTCTCTTCCGATTCCCTCGATGTGGGATAGTAAGAACTGGGATGGAGTTCTTGAGATGTTAACATCATGTCAAGCCAATCCCATCTCAACATCTCAGATGCATAAATGGATGGGAAGTTGGTTAATGTCTGATAATCATGATGCCAGTCAAGGGTATAGTTTTTTACATGAAGTGGACAAAGAGGCAGAAATAACATTTGACGTGGTGGAGACCTTCATCCGCGGCTGGGGCAACAAACCAATTGAATACATCAAAAAGGAAAGATGGACTGACTCATTCAAAATTCTCGCTTATTTGTGTCAAAAGTTTTTGGACTTACACAAGTTGACATTAATCTTAAATGCTGTCTCTGAGGTGGAATTGCTCAACTTGGCGAGGACTTTCAAAGGCAAAGTCAGAAGAAGTTCTCATGGAACGAACATATGCAGGATTAGGGTTCCCAGCTTGGGTCCTACTTTTATTTCAGAAGGATGGGCTTACTTCAAGAAACTTGATATTCTAATGGACCGAAACTTTCTGTTAATGGTCAAAGATGTGATTATAGGGAGGATGCAAACGGTGCTATCCATGGTATGTAGAATAGACAACCTGTTCTCAGAGCAAGACATCTTCTCCCTTCTAAATATCTACAGAATTGGAGATAAAATTGTGGAGAGGCAGGGAAATTTTTCTTATGACTTGATTAAAATGGTGGAACCGATATGCAACTTGAAGCTGATGAAATTAGCAAGAGAATCAAGGCCTTTAGTCCCACAATTCCCTCATTTTGAAAATCATATCAAGACTTCTGTTGATGAAGGGGCAAAAATTGACCGAGGTATAAGATTCCTCCATGATCAGATAATGAGTGTGAAAACAGTGGATCTCACACTGGTGATTTATGGATCGTTCAGACATTGGGGTCATCCTTTTATAGATTATTACACTGGACTAGAAAAATTACATTCCCAAGTAACCATGAAGAAAGATATTGATGTGTCATATGCAAAAGCACTTGCAAGTGATTTAGCTCGGATTGTTCTATTTCAACAGTTCAATGATCATAAAAAGTGGTTCGTGAATGGAGACTTGCTCCCTCATGATCATCCCTTTAAAAGTCATGTTAAAGAAAATACATGGCCCACAGCTGCTCAAGTTCAAGATTTTGGAGATAAATGGCATGAACTTCCGCTGATTAAATGTTTTGAAATACCCGACTTACTAGACCCATCGATAATATACTCTGACAAAAGTCATTCAATGAATAGGTCAGAGGTGTTGAAACATGTCCGAATGAATCCGAACACTCCTATCCCTAGTAAAAAGGTGTTGCAGACTATGTTGGACACAAAGGCTACCAATTGGAAAGAATTTCTTAAAGAGATTGATGAGAAGGGCTTAGATGATGATGATCTAATTATTGGTCTTAAAGGAAAGGAGAGGGAACTGAAGTTGGCAGGTAGATTTTTCTCCCTAATGTCTTGGAAATTGCGAGAATACTTTGTAATTACCGAATATTTGATAAAGACTCATTTCGTCCCTATGTTTAAAGGCCTGACAATGGCGGACGATCTAACTGCAGTCATTAAAAAGATGTTAGATTCCTCATCCGGCCAAGGATTGAAGTCATATGAGGCAATTTGCATAGCCAATCACATTGATTACGAAAAATGGAATAACCACCAAAGGAAGTTATCAAACGGCCCAGTGTTCCGAGTTATGGGCCAGTTCTTAGGTTATCCATCCTTAATCGAGAGAACTCATGAATTTTTTGAGAAAAGTCTTATATACTACAATGGAAGACCAGACTTGATGCGTGTTCACAACAACACACTGATCAATTCAACCTCCCAACGAGTTTGTTGGCAAGGACAAGAGGGTGGACTGGAAGGTCTACGGCAAAAAGGATGGACTATCCTCAATCTACTGGTTATTCAAAGAGAGGCTAAAATCAGAAACACTGCTGTCAAAGTCTTGGCACAAGGTGATAATCAAGTTATTTGCACACAGTATAAAACGAAGAAATCGAGAAACGTTGTAGAATTACAGGGTGCTCTCAATCAAATGGTTTCTAATAATGAGAAAATTATGACTGCAATCAAAATAGGGACAGGGAAGTTAGGACTTTTGATAAATGACGATGAGACTATGCAATCTGCAGATTACTTGAATTATGGAAAAATACCGATTTTCCGTGGAGTGATTAGAGGGTTAGAGACCAAGAGATGGTCACGAGTGACTTGTGTCACCAATGACCAAATACCCACTTGTGCTAATATAATGAGCTCAGTTTCCACAAATGCTCTCACCGTAGCTCATTTTGCTGAGAACCCAATCAATGCCATGATACAGTACAATTATTTTGGGACATTTGCTAGACTCTTGTTGATGATGCATGATCCTGCTCTTCGTCAATCATTGTATGAAGTTCAAGATAAGATACCGGGCTTGCACAGTTCTACTTTCAAATACGCCATGTTGTATTTGGACCCTTCCATTGGAGGAGTGTCGGGCATGTCTTTGTCCAGGTTTTTGATTAGAGCCTTCCCAGATCCCGTAACAGAAAGTCTCTCATTCTGGAGATTCATCCATGTACATGCTCGAAGTGAGCATCTGAAGGAGATGAGTGCAGTATTTGGAAACCCCGAGATAGCCAAGTTTCGAATAACTCACATAGACAAGCTAGTAGAAGATCCAACCTCTCTGAACATCGCTATGGGAATGAGTCCAGCGAACTTGTTAAAGACTGAGGTTAAAAAATGCTTAATCGAATCAAGACAAACCATCAGGAACCAGGTGATTAAGGATGCAACCATATATTTGTATCATGAAGAGGATCGGCTCAGAAGTTTCTTATGGTCAATAAATCCTCTGTTCCCTAGATTTTTAAGTGAATTCAAATCAGGCACTTTTTTGGGAGTCGCAGACGGGCTCATCAGTCTATTTCAAAATTCTCGTACTATTCGGAACTCCTTTAAGAAAAAGTATCATAGGGAATTGGATGATTTGATTGTGAGGAGTGAGGTATCCTCTTTGACACATTTAGGGAAACTTCATTTGAGAAGGGGATCATGTAAAATGTGGACATGTTCAGCTACTCATGCTGACACATTAAGATACAAATCCTGGGGCCGTACAGTTATTGGGACAACTGTACCCCATCCATTAGAAATGTTGGGTCCACAACATCGAAAAGAGACTCCTTGTGCACCATGTAACACATCAGGGTTCAATTATGTTTCTGTGCATTGTCCAGACGGGATCCATGACGTCTTTAGTTCACGGGGACCATTGCCTGCTTATCTAGGGTCTAAAACATCTGAATCTACATCTATTTTGCAGCCTTGGGAAAGGGAAAGCAAAGTCCCACTGATTAAAAGAGCTACACGTCTTAGAGATGCTATCTCTTGGTTTGTTGAACCCGACTCTAAACTAGCAATGACTATACTTTCTAACATCCACTCTTTAACAGGCGAAGAATGGACCAAAAGGCAGCATGGGTTCAAAAGAACAGGGTCTGCCCTTCATAGGTTTTCGACATCTCGGATGAGCCATGGTGGGTTCGCATCTCAGAGCACTGCAGCATTGACCAGGTTGATGGCAACTACAGACACCATGAGGGATCTGGGAGATCAGAATTTCGACTTTTTATTCCAAGCAACGTTGCTCTATGCTCAAATTACCACCACTGTTGCAAGAGACGGATGGATCACCAGTTGTACAGATCATTATCATATTGCCTGTAAGTCCTGTTTGAGACCCATAGAAGAGATCACCCTGGACTCAAGTATGGACTACACGCCCCCAGATGTATCCCATGTGCTGAAGACATGGAGGAATGGGGAAGGTTCGTGGGGACAAGAGATAAAACAGATCTATCCTTTAGAAGGGAATTGGAAGAATTTAGCACCTGCTGAGCAATCCTATCAAGTCGGCAGATGTATAGGTTTTCTATATGGAGACTTGGCGTATAGAAAATCTACTCATGCCGAGGACAGTTCTCTATTTCCTCTATCTATACAAGGTCGTATTAGAGGTCGAGGTTTCTTAAAAGGGTTGCTAGACGGATTAATGAGAGCAAGTTGCTGCCAAGTAATACACCGGAGAAGTCTGGCTCATTTGAAGAGGCCGGCCAACGCAGTGTACGGAGGTTTGATTTACTTGATTGATAAATTGAGTGTATCACCTCCATTCCTTTCTCTTACTAGATCAGGACCTATTAGAGACGAATTAGAAACGATTCCCCACAAGATCCCAACCTCCTATCCGACAAGCAACCGTGATATGGGGGTGATTGTCAGAAATTACTTCAAATACCAATGCCGTCTAATTGAAAAGGGAAAATACAGATCACATTATTCACAATTATGGTTATTCTCAGATGTCTTATCCATAGACTTCATTGGACCATTCTCTATTTCCACCACCCTCTTGCAAATCCTATACAAGCCATTTTTATCTGGGAAAGATAAGAATGAGTTGAGAGAGCTGGCAAATCTTTCTTCATTGCTAAGATCAGGAGAGGGGTGGGAAGACATACATGTGAAATTCTTCACCAAGGACATATTATTGTGTCCAGAGGAAATCAGACATGCTTGCAAGTTCGGGATTGCTAAGGATAATAATAAAGACATGAGCTATCCCCCTTGGGGAAGGGAATCCAGAGGGACAATTACAACAATCCCTGTTTATTATACGACCACCCCTTACCCAAAGATGCTAGAGATGCCTCCAAGAATCCAAAATCCCCTGCTGTCCGGAATCAGGTTGGGCCAATTACCAACTGGCGCTCATTATAAAATTCGGAGTATATTACATGGAATGGGAATCCATTACAGGGACTTCTTGAGTTGTGGAGACGGCTCCGGAGGGATGACTGCTGCATTACTACGAGAAAATGTGCATAGCAGAGGAATATTCAATAGTCTGTTAGAATTATCAGGGTCAGTCATGCGAGGCGCCTCTCCTGAGCCCCCCAGTGCCCTAGAAACTTTAGGAGGAGATAAATCGAGATGTGTAAATGGTGAAACATGTTGGGAATATCCATCTGACTTATGTGACCCAAGGACTTGGGACTATTTCCTCCGACTCAAAGCAGGCTTGGGGCTTCAAATTGATTTAATTGTAATGGATATGGAAGTTCGGGATTCTTCTACTAGCCTGAAAATTGAGACGAATGTTAGAAATTATGTGCACCGGATTTTGGATGAGCAAGGAGTTTTAATCTACAAGACTTATGGAACATATATTTGTGAGAGCGAAAAGAATGCAGTAACAATCCTTGGTCCCATGTTCAAGACGGTCGACTTAGTTCAAACAGAATTTAGTAGTTCTCAAACGTCTGAAGTATATATGGTATGTAAAGGTTTGAAGAAATTAATCGATGAACCCAATCCCGATTGGTCTTCCATCAATGAATCCTGGAAAAACCTGTACGCATTCCAGTCATCAGAACAGGAATTTGCCAGAGCAAAGAAGGTTAGTACATACTTTACCTTGACAGGTATTCCCTCCCAATTCATTCCTGATCCTTTTGTAAACATTGAGACTATGCTACAAATATTCGGAGTACCCACGGGTGTGTCTCATGCGGCTGCCTTAAAATCATCTGATAGACCTGCAGATTTATTGACCATTAGCCTTTTTTATATGGCGATTATATCGTATTATAACATCAATCATATCAGAGTAGGACCGATACCTCCGAACCCCCCATCAGATGGAATTGCACAAAATGTGGGGATCGCTATAACTGGTATAAGCTTTTGGCTGAGTTTGATGGAGAAAGACATTCCACTATATCAACAGTGTTTAGCAGTTATCCAGCAATCATTCCCGATTAGGTGGGAGGCTGTTTCAGTAAAAGGAGGATACAAGCAGAAGTGGAGTACTAGAGGTGATGGGCTCCCAAAAGATACCCGAACTTCAGACTCCTTGGCCCCAATCGGGAACTGGATCAGATCTCTGGAATTGGTCCGAAACCAAGTTCGTCTAAATCCATTCAATGAGATCTTGTTCAATCAGCTATGTCGTACAGTGGATAATCATTTGAAATGGTCAAATTTGCGAAGAAACACAGGAATGATTGAATGGATCAATAGACGAATTTCAAAAGAAGACCGGTCTATACTGATGTTGAAGAGTGACCTACACGAGGAAAACTCTTGGAGAGATTAAAAAATCATGAGGAGACTCCAAACTTTAAGTATGAAAAAAACTTTGATCCTTAAGACCCTCTTGTGGTTTTTATTTTTTATCTGGTTTTGTGGTCTTCGTGGGTCGGCATGGCATCTCCACCTCCTCGCGGTCCGACCTGGGCATCCGAAGGAGGACGCACGTCCACTCGGATGGCTAAGGGAGAGCCAGAAAATAACTAGTGGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGtCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCAC。
在一个实施方式中，核酸包括(a)编码狂犬病病毒基因组的至少一部分的序列和(b)编码包括SARS-CoV-2刺突蛋白(S)或其部分的蛋白质的序列。在一些实施方式中，狂犬病病毒基因组的至少一部分包括N基因和P基因，和编码包括SARS-CoV-2刺突蛋白(S)或其部分的蛋白质的序列被插入N基因和P基因之间的位置。在一些实施方式中，包括SARS-CoV-2刺突蛋白(S)或其部分的蛋白质是包括糖蛋白(G)或其部分和SARS-CoV-2刺突蛋白(S)或其部分的融合蛋白。在一些实施方式中，融合蛋白包括与SARS-CoV-2刺突蛋白(S)的S1结构域融合的糖蛋白(G)或其部分。
在另一个实施方式中，核酸包括SEQ ID NO：2的序列，如下所示：
CTGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAGcgcgccCTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACCCACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTAGATTAAGCGTCTGATGAGTCCGTGAGGACGAAACCCGGCGTACCGGGTCACGCTTAACAACCAGATCAAAGAAAAAACAGACATTGTCAATTGCAAAGCAAAAATGTAACACCCCTACAATGGATGCCGACAAGATTGTATTCAAAGTCAATAATCAGGTGGTCTCTTTGAAGCCTGAGATTATCGTGGATCAATATGAGTACAAGTACCCTGCCATCAAAGATTTGAAAAAGCCCTGTATAACCCTAGGAAAGGCTCCCGATTTAAATAAAGCATACAAGTCAGTTTTGTCAGGCATGAGCGCCGCCAAACTTAATCCTGACGATGTATGTTCCTATTTGGCAGCGGCAATGCAGTTTTTTGAGGGGACATGTCCGGAAGACTGGACCAGCTATGGAATTGTGATTGCACGAAAAGGAGATAAGATCACCCCAGGTTCTCTGGTGGAGATAAAACGTACTGATGTAGAAGGGAATTGGGCTCTGACAGGAGGCATGGAACTGACAAGAGACCCCACTGTCCCTGAGCATGCGTCCTTAGTCGGTCTTCTCTTGAGTCTGTATAGGTTGAGCAAAATATCCGGGCAAAACACTGGTAACTATAAGACAAACATTGCAGACAGGATAGAGCAGATTTTTGAGACAGCCCCTTTTGTTAAAATCGTGGAACACCATACTCTAATGACAACTCACAAaATGTGTGCTAATTGGAGTACTATACCAAACTTCAGATTTTTGGCCGGAACCTATGACATGTTTTTCTCCCGGATTGAGCATCTATATTCAGCAATCAGAGTGGGCACAGTTGTCACTGCTTATGAAGACTGTTCAGGACTGGTATCATTTACTGGGTTCATAAAACAAATCAATCTCACCGCTAGAGAGGCAATACTATATTTCTTCCACAAGAACTTTGAGGAAGAGATAAGAAGAATGTTTGAGCCAGGGCAGGAGACAGCTGTTCCTCACTCTTATTTCATCCACTTCCGTTCACTAGGCTTGAGTGGGAAATCTCCTTATTCATCAAATGCTGTTGGTCACGTGTTCAATCTCATTCACTTTGTAGGATGCTATATGGGTCAAGTCAGATCCCTAAATGCAACGGTTATTGCTGCATGTGCTCCTCATGAAATGTCTGTTCTAGGGGGCTATCTGGGAGAGGAATTCTTCGGGAAAGGGACATTTGAAAGAAGATTCTTCAGAGATGAGAAAGAACTTCAAGAATACGAGGCGGCTGAACTGACAAAGACTGACGTAGCACTGGCAGATGATGGAACTGTCAACTCTGACGACGAGGACTACTTTTCAGGTGAAACCAGAAGTCCGGAGGCTGTTTATACTCGAATCATGATGAATGGAGGTCGACTAAAGAGATCTCACATACGGAGATATGTCTCAGTCAGTTCCAATCATCAAGCCCGTCCAAACTCATTCGCCGAGTTTCTAAACAAGACATATTCGAGTGACTCATAAcatgaaaaaaactaacacccctcccGTACGCCACCATGTTCGTGTTTCTGGTGCTGCTGCCTCTGGTGAGCTCCCAGTGCGTGAACCTGACCACAAGGACCCAGCTGCCCCCTGCCTATACCAATTCCTTCACACGGGGCGTGTACTATCCCGACAAGGTGTTCCGGAGCAGCGTGCTGCACTCCACACAGGATCTGTTTCTGCCTTTCTTTTCTAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAATGGCACAAAGCGGTTCGACAATCCAGTGCTGCCCTTTAACGATGGCGTGTACTTCGCCTCCACCGAGAAGTCTAACATCATCAGAGGCTGGATCTTTGGCACCACACTGGACAGCAAGACACAGTCCCTGCTGATCGTGAACAATGCCACCAACGTGGTCATCAAGGTGTGCGAGTTCCAGTTTTGTAATGATCCATTCCTGGGCGTGTACTATCACAAGAACAATAAGTCTTGGATGGAGAGCGAGTTTCGCGTGTATTCCTCTGCCAACAATTGCACATTTGAGTACGTGTCCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAATTTCAAGAACCTGAGGGAGTTCGTGTTTAAGAATATCGATGGCTACTTCAAAATCTACTCCAAGCACACCCCAATCAACCTGGTGCGCGACCTGCCACAGGGCTTCTCTGCCCTGGAGCCACTGGTGGATCTGCCCATCGGCATCAACATCACCCGGTTTCAGACACTGCTGGCCCTGCACAGAAGCTACCTGACACCAGGCGACAGCTCCTCTGGATGGACCGCAGGAGCAGCAGCCTACTATGTGGGCTATCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAATGGCACCATCACAGACGCCGTGGATTGCGCCCTGGATCCCCTGTCTGAGACCAAGTGTACACTGAAGAGCTTTACCGTGGAGAAGGGCATCTATCAGACAAGCAATTTCAGGGTGCAGCCTACCGAGTCCATCGTGCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAAGATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGGAgGGCTTTAACTGTTATTTCCCTCTGCAGAgCTACgGCTTCCAGCCAACAAACGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACcTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGACTGACCGGAACAGGCGTGCTGACCGAGTCCAACAAGAAGTTCCTGCCTTTTCAGCAGTTCGGCAGGGACATCGCAGATACCACAGACGCCGTGCGCGACCCTCAGACCCTGGAGATCCTGGACATCACACCATGCTCCTTCGGCGGCGTGTCTGTGATCACACCAGGCACCAATACAAGCAACCAGGTGGCCGTGCTGTATCAGGACGTGAATTGTACCGAGGTGCCAGTGGCAATCCACGCAGATCAGCTGACCCCTACATGGCGGGTGTACTCTACCGGCAGCAACGTGTTCCAGACAAGAGCCGGATGCCTGATCGGAGCAGAGCACGTGAACAATAGCTATGAGTGCGACATCCCTATCGGCGCCGGCATCTGTGCCTCCTACCAGACCCAGACAAACTCCCCAAGGTCTGTGGGAGATGAGGCCGAAGACTTTGTGGAAGTCCACCTGCCTGATGTGCATAACCAGGTGTCTGGCGTCGACCTGGGACTGCCAAATTGGGGCAAGTACGTGCTGCTGAGTGCTGGAGCACTGACTGCCCTGATGCTGATCATTTTCCTGATGACCTGCTGTCGGCGCGTGAACAGAAGTGAGCCCACTCAGCACAATCTGCGAGGAACCGGGAGAGAAGTGTCAGTCACACCTCAGAGCGGGAAAATCATTAGTAGTTGGGAATCACATAAAAGCGGGGGCGAGACCAGGCTGTGAGCTAGCCATGAAAAAAACTAACACCCCTCCTTTCGAACCATCCCAAACATGAGCAAGATCTTTGTCAATCCTAGTGCTATTAGAGCCGGTCTGGCCGATCTTGAGATGGCTGAAGAAACTGTTGATCTGATCAATAGAAATATCGAAGACAATCAGGCTCATCTCCAAGGGGAACCCATAGAGGTGGACAATCTCCCTGAGGATATGGGGCGACTTCACCTGGATGATGGAAAATCGCCCAACCATGGTGAGATAGCCAAGGTGGGAGAAGGCAAGTATCGAGAGGACTTTCAGATGGATGAAGGAGAGGATCCTAGCTTCCTGTTCCAGTCATACCTGGAAAATGTTGGAGTCCAAATAGTCAGACAAATGAGGTCAGGAGAGAGATTTCTCAAGATATGGTCACAGACCGTAGAAGAGATTATATCCTATGTCGCGGTCAACTTTCCCAACCCTCCAGGAAAGTCTTCAGAGGATAAATCAACCCAGACTACTGGCCGAGAGCTCAAGAAGGAGACAACACCCACTCCTTCTCAGAGAGAAAGCCAATCATCGAAAGCCAGGATGGCGGCTCAAATTGCTTCTGGCCCTCCAGCCCTTGAATGGTCGGCTACCAATGAAGAGGATGATCTATCAGTGGAGGCTGAGATCGCTCACCAGATTGCAGAAAGTTTCTCCAAAAAATATAAGTTTCCCTCTCGATCCTCAGGGATACTCTTGTATAATTTTGAGCAATTGAAAATGAACCTTGATGATATAGTTAAAGAGGCAAAAAATGTACCAGGTGTGACCCGTTTAGCCCATGACGGGTCCAAACTCCCCCTAAGATGTGTACTGGGATGGGTCGCTTTGGCCAACTCTAAGAAATTCCAGTTGTTAGTCGAATCCGACAAGCTGAGTAAAATCATGCAAGATGACTTGAATCGCTATACATCTTGCTAACCGAACCTCTCCCCTCAGTCCCTCTAGACAATAAAATCCGAGATGTCCCAAAGTCAACATGAAAAAAACAGGCAACACCACTGATAAAATGAACCTCCTACGTAAGATAGTGAAAAACCGCAGGGACGAGGACACTCAAAAATCCTCTCCCGCGTCAGCCCCTCTGGATGACGATGACTTGTGGCTTCCACCCCCTGAATACGTCCCGCTGAAAGAACTTACAGGCAAGAAGAACATGAGGAACTTTTGTATCAACGGAAGGGTTAAAGTGTGTAGCCCGAATGGTTACTCGTTCAGGATCCTGCGGCACATTCTGAAATCATTCGACGAGATATATTCTGGGAATCATAGGATGATCGGGTTAGTCAAAGTGGTTATTGGACTGGCTTTGTCAGGATCTCCAGTCCCTGAGGGCCTGAACTGGGTATACAAATTGAGGAGAACCTTTATCTTCCAGTGGGCTGATTCCAGGGGCCCTCTTGAAGGGGAGGAGTTGGAATACTCTCAGGAGATCACTTGGGATGATGATACTGAGTTCGTCGGATTGCAAATAAGAGTGATTGCAAAACAGTGTCATATCCAGGGCAGAGTCTGGTGTATCAACATGAACCCGAGAGCATGTCAACTATGGTCTGACATGTCTCTTCAGACACAAAGGTCCGAAGAGGACAAAGATTCCTCTCTGCTTCTAGAATAATCAGATTATATCCCGCAAATTTATCACTTGTTTACCTCTGGAGGAGAGAACATATGGGCTCAACTCCAACCCTTGGGAGCAATATAACAAAAAACATGTTATGGTGCCATTAAACCGCTGCATTTCATCAAAGTCAAGTTGATTACCTTTACATTTTGATCCTCTTGGATGTGAAAAAAACTATTAACATCCCTCAAAAGACcccgggAAAGATGGTTCCTCAGGCTCTCCTGTTTGTACCCCTTCTGGTTTTTCCATTGTGTTTTGGGAAATTCCCTATTTACACGATACCAGACAAGCTTGGTCCCTGGAGTCCGATTGACATACATCACCTCAGCTGCCCAAACAATTTGGTAGTGGAGGACGAAGGATGCACCAACCTGTCAGGGTTCTCCTACATGGAACTTAAAGTTGGATACATCTTAGCCATAAAAGTGAACGGGTTCACTTGCACAGGCGTTGTGACGGAGGCTGAAACCTACACTAACTTCGTTGGTTATGTCACAACCACGTTCAAAAGAAAGCATTTCCGCCCAACACCAGATGCATGTAGAGCCGCGTACAACTGGAAGATGGCCGGTGACCCCAGATATGAAGAGTCTCTACACAATCCGTACCCTGACTACCGCTGGCTTCGAACTGTAAAAACCACCAAGGAGTCTCTCGTTATCATATCTCCAAGTGTGGCAGATTTGGACCCATATGACAGATCCCTTCACTCGAGGGTCTTCCCTAGCGGGAAGTGCTCAGGAGTAGCGGTGTCTTCTACCTACTGCTCCACTAACCACGATTACACCATTTGGATGCCCGAGAATCCGAGACTAGGGATGTCTTGTGACATTTTTACCAATAGTAGAGGGAAGAGAGCATCCAAAGGGAGTGAGACTTGCGGCTTTGTAGATGAAAGAGGCCTATATAAGTCTTTAAAAGGAGCATGCAAACTCAAGTTATGTGGAGTTCTAGGACTTAGACTTATGGATGGAACATGGGTCTCGATGCAAACATCAAATGAAACCAAATGGTGCCCTCCCGATAAGTTGGTGAACCTGCACGACTTTCGCTCAGACGAAATTGAGCACCTTGTTGTAGAGGAGTTGGTCAGGAAGAGAGAGGAGTGTCTGGATGCACTAGAGTCCATCATGACAACCAAGTCAGTGAGTTTCAGACGTCTCAGTCATTTAAGAAAACTTGTCCCTGGGTTTGGAAAAGCATATACCATATTCAACAAGACCTTGATGGAAGCCGATGCTCACTACAAGTCAGTCgagACTTGGAATGAGATCCTCCCTTCAAAAGGGTGTTTAAGAGTTGGGGGGAGGTGTCATCCTCATGTGAACGGGGTGTTTTTCAATGGTATAATATTAGGACCTGACGGCAATGTCTTAATCCCAGAGATGCAATCATCCCTCCTCCAGCAACATATGGAGTTGTTGGAATCCTCGGTTATCCCCCTTGTGCACCCCCTGGCAGACCCGTCTACCGTTTTCAAGGACGGTGACGAGGCTGAGGATTTTGTTGAAGTTCACCTTCCCGATGTGCACAATCAGGTCTCAGGAGTTGACTTGGGTCTCCCGAACTGGGGGAAGTATGTATTACTGAGTGCAGGGGCCCTGACTGCCTTGATGTTGATAATTTTCCTGATGACATGTTGTAGAAGAGTCAATCGATCAGAACCTACGCAACACAATCTCAGAGGGACAGGGAGGGAGGTGTCAGTCACTCCCCAAAGCGGGAAGATCATATCTTCATGGGAATCACACAAGAGTGGGGGTGAGACCAGACTGTAAttaattaaCGTCCTTTCAACGATCCAAGTCcatgaaaaaaactaacacccctcccgtacctagcTTATAAAGTGCTGGGTCATCTAAGCTTTTCAGTCGAGAAAAAAACATTAGATCAGAAGAACAACTGGCAACACTTCTCAACCTGAGACTTACTTCAAGATGCTCGATCCTGGAGAGGTCTATGATGACCCTATTGACCCAATCGAGTTAGAGGCTGAACCCAGAGGAACCCCCATTGTCCCCAACATCTTGAGGAACTCTGACTACAATCTCAACTCTCCTTTGATAGAAGATCCTGCTAGACTAATGTTAGAATGGTTAAAAACAGGGAATAGACCTTATCGGATGACTCTAACAGACAATTGCTCCAGGTCTTTCAGAGTTTTGAAAGATTATTTCAAGAAGGTAGATTTGGGTTCTCTCAAGGTGGGCGGAATGGCTGCACAGTCAATGATTTCTCTCTGGTTATATGGTGCCCACTCTGAATCCAACAGGAGCCGGAGATGTATAACAGACTTGGCCCATTTCTATTCCAAGTCGTCCCCCATAGAGAAGCTGTTGAATCTCACGCTAGGAAATAGAGGGCTGAGAATCCCCCCAGAGGGAGTGTTAAGTTGCCTTGAGAGGGTTGATTATGATAATGCATTTGGAAGGTATCTTGCCAACACGTATTCCTCTTACTTGTTCTTCCATGTAATCACCTTATACATGAACGCCCTAGACTGGGATGAAGAAAAGACCATCCTAGCATTATGGAAAGATTTAACCTCAGTGGACATCGGGAAGGACTTGGTAAAGTTCAAAGACCAAATATGGGGACTGCTGATCGTGACAAAGGACTTTGTTTACTCCCAAAGTTCCAATTGTCTTTTTGACAGAAACTACACACTTATGCTAAAAGATCTTTTCTTGTCTCGCTTCAACTCCTTAATGGTCTTGCTCTCTCCCCCAGAGCCCCGATACTCAGATGACTTGATATCTCAACTATGCCAGCTGTACATTGCTGGGGATCAAGTCTTGTCTATGTGTGGAAACTCCGGCTATGAAGTCATCAAAATATTGGAGCCATATGTCGTGAATAGTTTAGTCCAGAGAGCAGAAAAGTTTAGGCCTCTCATTCATTCCTTGGGAGACTTTCCTGTATTTATAAAAGACAAGGTAAGTCAACTTGAAGAGACGTTCGGTCCCTGTGCAAGAAGGTTCTTTAGGGCTCTGGATCAATTCGACAACATACATGACTTGGTTTTTGTGTTTGGCTGTTACAGGCATTGGGGGCACCCATATATAGATTATCGAAAGGGTCTGTCAAAACTATATGATCAGGTTCACCTTAAAAAAATGATAGATAAGTCCTACCAGGAGTGCTTAGCAAGCGACCTAGCCAGGAGGATCCTTAGATGGGGTTTTGATAAGTACTCCAAGTGGTATCTGGATTCAAGATTCCTAGCCCGAGACCACCCCTTGACTCCTTATATCAAAACCCAAACATGGCCACCCAAACATATTGTAGACTTGGTGGGGGATACATGGCACAAGCTCCCGATCACGCAGATCTTTGAGATTCCTGAATCAATGGATCCGTCAGAAATATTGGATGACAAATCACATTCTTTCACCAGAACGAGACTAGCTTCTTGGCTGTCAGAAAACCGAGGGGGGCCTGTTCCTAGCGAAAAAGTTATTATCACGGCCCTGTCTAAGCCGCCTGTCAATCCCCGAGAGTTTCTGAGGTCTATAGACCTCGGAGGATTGCCAGATGAAGACTTGATAATTGGCCTCAAGCCAAAGGAACGGGAATTGAAGATTGAAGGTCGATTCTTTGCTCTAATGTCATGGAATCTAAGATTGTATTTTGTCATCACTGAAAAACTCTTGGCCAACTACATCTTGCCACTTTTTGACGCGCTGACTATGACAGACAACCTGAACAAGGTGTTTAAAAAGCTGATCGACAGGGTCACCGGGCAAGGGCTTTTGGACTATTCAAGGGTCACATATGCATTTCACCTGGACTATGAAAAGTGGAACAACCATCAAAGATTAGAGTCAACAGAGGATGTATTTTCTGTCCTAGATCAAGTGTTTGGATTGAAGAGAGTGTTTTCTAGAACACACGAGTTTTTTCAAAAGGCCTGGATCTATTATTCAGACAGATCAGACCTCATCGGGTTACGGGAGGATCAAATATACTGCTTAGATGCGTCCAACGGCCCAACCTGTTGGAATGGCCAGGATGGCGGGCTAGAAGGCTTACGGCAGAAGGGCTGGAGTCTAGTCAGCTTATTGATGATAGATAGAGAATCTCAAATCAGGAACACAAGAACCAAAATACTAGCTCAAGGAGACAACCAGGTTTTATGTCCGACATACATGTTGTCGCCAGGGCTATCTCAAGAGGGGCTCCTCTATGAATTGGAGAGAATATCAAGGAATGCACTTTCGATATACAGAGCCGTCGAGGAAGGGGCATCTAAGCTAGGGCTGATCATCAAGAAAGAAGAGACCATGTGTAGTTATGACTTCCTCATCTATGGAAAAACCCCTTTGTTTAGAGGTAACATATTGGTGCCTGAGTCCAAAAGATGGGCCAGAGTCTCTTGCGTCTCTAATGACCAAATAGTCAACCTCGCCAATATAATGTCGACAGTGTCCACCAATGCGCTAACAGTGGCACAACACTCTCAATCTTTGATCAAACCGATGAGGGATTTTCTGCTCATGTCAGTACAGGCAGTCTTTCACTACCTGCTATTTAGCCCAATCTTAAAGGGAAGAGTTTACAAGATTCTGAGCGCTGAAGGGGAGAGCTTTCTCCTAGCCATGTCAAGGATAATCTATCTAGATCCTTCTTTGGGAGGGATATCTGGAATGTCCCTCGGAAGATTCCATATACGACAGTTCTCAGACCCTGTCTCTGAAGGGTTATCCTTCTGGAGAGAGATCTGGTTAAGCTCCCAAGAGTCCTGGATTCACGCGTTGTGTCAAGAGGCTGGAAACCCAGATCTTGGAGAGAGAACACTCGAGAGCTTCACTCGCCTTCTAGAAGATCCGACCACCTTAAATATCAGAGGAGGGGCCAGTCCTACCATTCTACTCAAGGATGCAATCAGAAAGGCTTTATATGACGAGGTGGACAAGGTGGAAAATTCAGAGTTTCGAGAGGCAATCCTGTTGTCCAAGACCCATAGAGATAATTTTATACTCTTCTTAATATCTGTTGAGCCTCTGTTTCCTCGATTTCTCAGTGAGCTATTCAGTTCGTCTTTTTTGGGAATCCCCGAGTCAATCATTGGATTGATACAAAACTCCCGAACGATAAGAAGGCAGTTTAGAAAGAGTCTCTCAAAAACTTTAGAAGAATCCTTCTACAACTCAGAGATCCACGGGATTAGTCGGATGACCCAGACACCTCAGAGGGTTGGGGGGGTGTGGCCTTGCTCTTCAGAGAGGGCAGATCTACTTAGGGAGATCTCTTGGGGAAGAAAAGTGGTAGGCACGACAGTTCCTCACCCTTCTGAGATGTTGGGATTACTTCCCAAGTCCTCTATTTCTTGCACTTGTGGAGCAACAGGAGGAGGCAATCCTAGAGTTTCTGTATCAGTACTCCCGTCCTTTGATCAGTCATTTTTTTCACGAGGCCCCCTAAAGGGATACTTGGGCTCGTCCACCTCTATGTCGACCCAGCTATTCCATGCATGGGAAAAAGTCACTAATGTTCATGTGGTGAAGAGAGCTCTATCGTTAAAAGAATCTATAAACTGGTTCATTACTAGAGATTCCAACTTGGCTCAAGCTCTAATTAGGAACATTATGTCTCTGACAGGCCCTGATTTCCCTCTAGAGGAGGCCCCTGTCTTCAAAAGGACGGGGTCAGCCTTGCATAGGTTCAAGTCTGCCAGATACAGCGAAGGAGGGTATTCTTCTGTCTGCCCGAACCTCCTCTCTCATATTTCTGTTAGTACAGACACCATGTCTGATTTGACCCAAGACGGGAAGAACTACGATTTCATGTTCCAGCCATTGATGCTTTATGCACAGACATGGACATCAGAGCTGGTACAGAGAGACACAAGGCTAAGAGACTCTACGTTTCATTGGCACCTCCGATGCAACAGGTGTGTGAGACCCATTGACGACGTGACCCTGGAGACCTCTCAGATCTTCGAGTTTCCGGATGTGTCGAAAAGAATATCCAGAATGGTTTCTGGGGCTGTGCCTCACTTCCAGAGGCTTCCCGATATCCGTCTGAGACCAGGAGATTTTGAATCTCTAAGCGGTAGAGAAAAGTCTCACCATATCGGATCAGCTCAGGGGCTCTTATACTCAATCTTAGTGGCAATTCACGACTCAGGATACAATGATGGAACCATCTTCCCTGTCAACATATACGGCAAGGTTTCCCCTAGAGACTATTTGAGAGGGCTCGCAAGGGGAGTATTGATAGGATCCTCGATTTGCTTCTTGACAAGAATGACAAATATCAATATTAATAGACCTCTTGAATTGGTCTCAGGGGTAATCTCATATATTCTCCTGAGGCTAGATAACCATCCCTCCTTGTACATAATGCTCAGAGAACCGTCTCTTAGAGGAGAGATATTTTCTATCCCTCAGAAAATCCCCGCCGCTTATCCAACCACTATGAAAGAAGGCAACAGATCAATCTTGTGTTATCTCCAACATGTGCTACGCTATGAGCGAGAGATAATCACGGCGTCTCCAGAGAATGACTGGCTATGGATCTTTTCAGACTTTAGAAGTGCCAAAATGACGTACCTATCCCTCATTACTTACCAGTCTCATCTTCTACTCCAGAGGGTTGAGAGAAACCTATCTAAGAGTATGAGAGATAACCTGCGACAATTGAGTTCTTTGATGAGGCAGGTGCTGGGCGGGCACGGAGAAGATACCTTAGAGTCAGACGACAACATTCAACGACTGCTAAAAGACTCTTTACGAAGGACAAGATGGGTGGATCAAGAGGTGCGCCATGCAGCTAGAACCATGACTGGAGATTACAGCCCCAACAAGAAGGTGTCCCGTAAGGTAGGATGTTCAGAATGGGTCTGCTCTGCTCAACAGGTTGCAGTCTCTACCTCAGCAAACCCGGCCCCTGTCTCGGAGCTTGACATAAGGGCCCTCTCTAAGAGGTTCCAGAACCCTTTGATCTCGGGCTTGAGAGTGGTTCAGTGGGCAACCGGTGCTCATTATAAGCTTAAGCCTATTCTAGATGATCTCAATGTTTTCCCATCTCTCTGCCTTGTAGTTGGGGACGGGTCAGGGGGGATATCAAGGGCAGTCCTCAACATGTTTCCAGATGCCAAGCTTGTGTTCAACAGTCTTTTAGAGGTGAATGACCTGATGGCTTCCGGAACACATCCACTGCCTCCTTCAGCAATCATGAGGGGAGGAAATGATATCGTCTCCAGAGTGATAGATCTTGACTCAATCTGGGAAAAACCGTCCGACTTGAGAAACTTGGCAACCTGGAAATACTTCCAGTCAGTCCAAAAGCAGGTCAACATGTCCTATGACCTCATTATTTGCGATGCAGAAGTTACTGACATTGCATCTATCAACCGGATCACCCTGTTAATGTCCGATTTTGCATTGTCTATAGATGGACCACTCTATTTGGTCTTCAAAACTTATGGGACTATGCTAGTAAATCCAAACTACAAGGCTATTCAACACCTGTCAAGAGCGTTCCCCTCGGTCACAGGGTTTATCACCCAAGTAACTTCGTCTTTTTCATCTGAGCTCTACCTCCGATTCTCCAAACGAGGGAAGTTTTTCAGAGATGCTGAGTACTTGACCTCTTCCACCCTTCGAGAAATGAGCCTTGTGTTATTCAATTGTAGCAGCCCCAAGAGTGAGATGCAGAGAGCTCGTTCCTTGAACTATCAGGATCTTGTGAGAGGATTTCCTGAAGAAATCATATCAAATCCTTACAATGAGATGATCATAACTCTGATTGACAGTGATGTAGAATCTTTTCTAGTCCACAAGATGGTTGATGATCTTGAGTTACAGAGGGGAACTCTGTCTAAAGTGGCTATCATTATAGCCATCATGATAGTTTTCTCCAACAGAGTCTTCAACGTTTCCAAACCCCTAACTGACCCCTCGTTCTATCCACCGTCTGATCCCAAAATCCTGAGGCACTTCAACATATGTTGCAGTACTATGATGTATCTATCTACTGCTTTAGGTGACGTCCCTAGCTTCGCAAGACTTCACGACCTGTATAACAGACCTATAACTTATTACTTCAGAAAGCAAGTCATTCGAGGGAACGTTTATCTATCTTGGAGTTGGTCCAACGACACCTCAGTGTTCAAAAGGGTAGCCTGTAATTCTAGCCTGAGTCTGTCATCTCACTGGATCAGGTTGATTTACAAGATAGTGAAGACTACCAGACTCGTTGGCAGCATCAAGGATCTATCCAGAGAAGTGGAAAGACACCTTCATAGGTACAACAGGTGGATCACCCTAGAGGATATCAGATCTAGATCATCCCTACTAGACTACAGTTGCCTGTGAACCGGATACTCCTGGAAGCCTGCCCATGCTAAGACTCTTGTGTGATGTATCTTGAAAAAAACAAGATCCTAAATCTGAACCTTTGGTTGTTTGATTGTTTTTCTCAtttttgttgtttatttgttaagcgtGGGTCGGCATGGCATCTCCACCTCCTCGCGGTCCGACCTGGGCATCCGAAGGAGGACGCACGTCCACTCGGATGGCTAAGGGAGagccagaaGGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGtCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTaCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCAC。
在一个实施方式中，核酸包括编码麻疹病毒基因组的至少一部分的序列和(b)编码SARS-CoV-2刺突蛋白(S)或其部分的序列。在一些实施方式中，编码SARS-CoV-2刺突蛋白(S)或其部分的序列被插入麻疹病毒基因组的第2、3或6位。
在一个实施方式中，核酸包括SEQ ID NO：3的序列，如下所示：
GGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGCGCGCCGTTAATACGACTCACTATAGGGAGACCCAAGCTGGCTAGCTTTGTTTGGTCTGATGAGTCCCGTGAGGACGAAACCCGGCGTACCGGGTCACCAAACAAAGTTGGGTAAGGATAGTTCAATCAATGATCATCTTCTAGTGCACTTAGGATTCAAGATCCTATTATCAGGGACAAGAGCAGGATTAGGGATATCCGAGATGGCCACACTTTTAAGGAGCTTAGCATTGTTCAAAAGAAACAAGGACAAACCACCCATTACATCAGGATCCGGTGGAGCCATCAGAGGAATCAAACACATTATTATAGTACCAATCCCTGGAGATTCCTCAATTACCACTCGATCCAGACTTCTGGACCGGTTGGTGAGGTTAATTGGAAACCCGGATGTGAGCGGGCCCAAACTAACAGGGGCACTAATAGGTATATTATCCTTATTTGTGGAGTCTCCAGGTCAATTGATTCAGAGGATCACCGATGACCCTGACGTTAGCATAAGGCTGTTAGAGGTTGTCCAGAGTGACCAGTCACAATCTGGCCTTACCTTCGCATCAAGAGGTACCAACATGGAGGATGAGGCGGACCAATACTTTTCACATGATGATCCAATTAGTAGTGATCAATCCAGGTTCGGATGGTTCGGGAACAAGGAAATCTCAGATATTGAAGTGCAAGACCCTGAGGGATTCAACATGATTCTGGGTACCATCCTAGCCCAAATTTGGGTCTTGCTCGCAAAGGCGGTTACGGCCCCAGACACGGCAGCTGATTCGGAGCTAAGAAGGTGGATAAAGTACACCCAACAAAGAAGGGTAGTTGGTGAATTTAGATTGGAGAGAAAATGGTTGGATGTGGTGAGGAACAGGATTGCCGAGGACCTCTCCTTACGCCGATTCATGGTCGCTCTAATCCTGGATATCAAGAGAACACCCGGAAACAAACCCAGGATTGCTGAAATGATATGTGACATTGATACATATATCGTAGAGGCAGGATTAGCCAGTTTTATCCTGACTATTAAGTTTGGGATAGAAACTATGTATCCTGCTCTTGGACTGCATGAATTTGCTGGTGAGTTATCCACACTTGAGTCCTTGATGAACCTTTACCAGCAAATGGGGGAAACTGCACCCTACATGGTAATCCTGGAGAACTCAATTCAGAACAAGTTCAGTGCAGGATCATACCCTCTGCTCTGGAGCTATGCCATGGGAGTAGGAGTGGAACTTGAAAACTCCATGGGAGGTTTGAACTTTGGCCGATCTTACTTTGATCCAGCATATTTTAGATTAGGGCAAGAGATGGTAAGGAGGTCAGCTGGAAAGGTCAGTTCCACATTGGCATCTGAACTCGGTATCACTGCCGAGGATGCAAGGCTTGTTTCAGAGATTGCAATGCATACTACTGAGGACAAGATCAGTAGAGCGGTTGGACCCAGACAAGCCCAAGTATCATTTCTACACGGTGATCAAAGTGAGAATGAGCTACCGAGATTGGGGGGCAAGGAAGATAGGAGGGTCAAACAGAGTCGAGGAGAAGCCAGGGAGAGCTACAGAGAAACCGGGCCCAGCAGAGCAAGTGATGCGAGAGCTGCCCATCTTCCAACCGGCACACCCCTAGACATTGACACTGCAACGGAGTCCAGCCAAGATCCGCAGGACAGTCGAAGGTCAGCTGACGCCCTGCTTAGGCTGCAAGCCATGGCAGGAATCTCGGAAGAACAAGGCTCAGACACGGACACCCCTATAGTGTACAATGACAGAAATCTTCTAGACTAGGTGCGAGAGGCCGAGGGCCAGAACAACATCCGCCTACCATCCATCATTGTTATAAAAAACTTAGGAACCAggtccacacaGagtgatACGCGTACGCCACCATGTTCGTGTTTCTGGTGCTGCTGCCTCTGGTGAGCTCCCAGTGCGTGAACCTGACCACAAGGACCCAGCTGCCCCCTGCCTATACCAATTCCTTCACACGGGGCGTGTACTATCCCGACAAGGTGTTCCGGAGCAGCGTGCTGCACTCCACACAGGATCTGTTTCTGCCTTTCTTTTCTAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAATGGCACAAAGCGGTTCGACAATCCAGTGCTGCCCTTTAACGATGGCGTGTACTTCGCCTCCACCGAGAAGTCTAACATCATCAGAGGCTGGATCTTTGGCACCACACTGGACAGCAAGACACAGTCCCTGCTGATCGTGAACAATGCCACCAACGTGGTCATCAAGGTGTGCGAGTTCCAGTTTTGTAATGATCCATTCCTGGGCGTGTACTATCACAAGAACAATAAGTCTTGGATGGAGAGCGAGTTTCGCGTGTATTCCTCTGCCAACAATTGCACATTTGAGTACGTGTCCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAATTTCAAGAACCTGAGGGAGTTCGTGTTTAAGAATATCGATGGCTACTTCAAAATCTACTCCAAGCACACCCCAATCAACCTGGTGCGCGACCTGCCACAGGGCTTCTCTGCCCTGGAGCCACTGGTGGATCTGCCCATCGGCATCAACATCACCCGGTTTCAGACACTGCTGGCCCTGCACAGAAGCTACCTGACACCAGGCGACAGCTCCTCTGGATGGACCGCAGGAGCAGCAGCCTACTATGTGGGCTATCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAATGGCACCATCACAGACGCCGTGGATTGCGCCCTGGATCCCCTGTCTGAGACCAAGTGTACACTGAAGAGCTTTACCGTGGAGAAGGGCATCTATCAGACAAGCAATTTCAGGGTGCAGCCTACCGAGTCCATCGTGCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAAGATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGGAGGGCTTTAACTGTTATTTCCCTCTGCAGAGCTACGGCTTCCAGCCAACAAACGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACCTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGACTGACCGGAACAGGCGTGCTGACCGAGTCCAACAAGAAGTTCCTGCCTTTTCAGCAGTTCGGCAGGGACATCGCAGATACCACAGACGCCGTGCGCGACCCTCAGACCCTGGAGATCCTGGACATCACACCATGCTCCTTCGGCGGCGTGTCTGTGATCACACCAGGCACCAATACAAGCAACCAGGTGGCCGTGCTGTATCAGGACGTGAATTGTACCGAGGTGCCAGTGGCAATCCACGCAGATCAGCTGACCCCTACATGGCGGGTGTACTCTACCGGCAGCAACGTGTTCCAGACAAGAGCCGGATGCCTGATCGGAGCAGAGCACGTGAACAATAGCTATGAGTGCGACATCCCTATCGGCGCCGGCATCTGTGCCTCCTACCAGACCCAGACAAACTCCCCAAGGAGAGCACGGTCTGTGGCCAGCCAGTCCATCATCGCCTATACCATGAGCCTGGGCGCCGAGAATTCCGTGGCCTACTCCAACAATTCTATCGCCATCCCTACCAACTTCACAATCTCCGTGACCACAGAGATCCTGCCAGTGAGCATGACCAAGACATCCGTGGACTGCACAATGTATATCTGTGGCGATTCCACCGAGTGCTCTAACCTGCTGCTGCAGTACGGCTCTTTTTGTACCCAGCTGAATAGAGCCCTGACAGGCATCGCCGTGGAGCAGGACAAGAACACACAGGAGGTGTTCGCCCAGGTGAAGCAAATCTACAAGACCCCACCCATCAAGGACTTTGGCGGCTTCAACTTCAGCCAGATCCTGCCCGATCCTAGCAAGCCATCCAAGCGGTCTTTTATCGAGGACCTGCTGTTCAACAAGGTGACCCTGGCCGATGCCGGCTTCATCAAGCAGTATGGCGATTGCCTGGGCGACATCGCCGCCAGAGACCTGATCTGTGCCCAGAAGTTTAATGGCCTGACCGTGCTGCCTCCACTGCTGACAGATGAGATGATCGCCCAGTACACATCTGCCCTGCTGGCCGGAACCATCACAAGCGGATGGACCTTCGGCGCAGGAGCCGCCCTGCAGATCCCCTTTGCCATGCAGATGGCCTATCGGTTCAACGGCATCGGCGTGACCCAGAATGTGCTGTACGAGAACCAGAAGCTGATCGCCAATCAGTTTAACTCCGCCATCGGCAAGATCCAGGACTCTCTGAGCTCCACAGCCAGCGCCCTGGGCAAGCTGCAGGATGTGGTGAATCAGAACGCCCAGGCCCTGAATACCCTGGTGAAGCAGCTGTCTAGCAACTTCGGCGCCATCTCCTCTGTGCTGAATGACATCCTGAGCCGGCTGGACAAGGTGGAGGCAGAGGTGCAGATCGACCGGCTGATCACAGGCAGACTGCAGTCCCTGCAGACCTACGTGACACAGCAGCTGATCAGGGCAGCAGAGATCAGGGCCTCTGCCAATCTGGCCGCCACCAAGATGAGCGAGTGCGTGCTGGGCCAGTCCAAGAGAGTGGACTTTTGTGGCAAGGGCTATCACCTGATGAGCTTCCCACAGTCCGCCCCTCACGGAGTGGTGTTTCTGCACGTGACCTACGTGCCAGCCCAGGAGAAGAACTTCACCACAGCACCAGCAATCTGCCACGATGGCAAGGCACACTTTCCTAGGGAGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTTGTGACACAGCGCAATTTCTACGAGCCACAGATCATCACCACAGACAATACATTCGTGTCCGGCAACTGTGACGTGGTCATCGGCATCGTGAACAATACCGTGTATGATCCTCTGCAGCCAGAGCTGGACTCTTTTAAGGAGGAGCTGGATAAGTACTTCAAGAATCACACCAGCCCCGACGTGGATCTGGGCGACATCTCTGGCATCAATGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAGGTGGCCAAGAATCTGAACGAGTCCCTGATCGATCTGCAGGAGCTGGGCAAGTATGAGCAGTACATCAAGTGGCCCTGGTATATCTGGCTGGGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTGTATGACAAGCTGCTGTTCCTGCCTGAAGGGCTGCTGTTCTTGTGGCAGCTGCTGTAAGTTTGATGAGGACGATAGCGAGCCTGTGCTGAAGGGCGTGAAGCTGCACTACACCTGATAGCTAGCGATCGCGTGCGAGAGGCCAGAACAACATCCGCCTACCATCCATCATTGTTATAAAAAACTTAGGAACCAGGTCCACACAGCCGCCAGCCCATCAACCATCCACTCCCACGATTGGAGCCAATGGCAGAAGAGCAGGCACGCCATGTCAAAAACGGACTGGAATGCATCCGGGCTCTCAAGGCCGAGCCCATCGGCTCACTGGCCATCGAGGAAGCTATGGCAGCATGGTCAGAAATATCAGACAACCCAGGACAGGAGCGAGCCACCTGCAGGGAAGAGAAGGCAGGCAGTTCGGGTCTCAGCAAACCATGCCTCTCAGCAATTGGATCAACTGAAGGCGGTGCACCTCGCATCCGCGGTCAGGGACCTGGAGAGAGCGATGACGACGCTGAAACTTTGGGAATCCCCCCAAGAAATCTCCAGGCATCAAGCACTGGGTTACAGTGTTATTACGTTTATGATCACAGCGGTGAAGCGGTTAAGGGAATCCAAGATGCTGACTCTATCATGGTTCAATCAGGCCTTGATGGTGATAGCACCCTCTCAGGAGGAGACAATGAATCTGAAAACAGCGATGTGGATATTGGCGAACCTGATACCGAGGGATATGCTATCACTGACCGGGGATCTGCTCCCATCTCTATGGGGTTCAGGGCTTCTGATGTTGAAACTGCAGAAGGAGGGGAGATCCACGAGCTCCTGAGACTCCAATCCAGAGGCAACAACTTTCCGAAGCTTGGGAAAACTCTCAATGTTCCTCCGCCCCCGGACCCCGGTAGGGCCAGCACTTCCGGGACACCCATTAAAAAGGGCACAGACGCGAGATTAGCCTCATTTGGAACGGAGATCGCGTCTTTATTGACAGGTGGTGCAACCCAATGTGCTCGAAAGTCACCCTCGGAACCATCAGGGCCAGGTGCACCTGCGGGGAATGTCCCCGAGTGTGTGAGCAATGCCGCACTGATACAGGAGTGGACACCCGAATCTGGTACCACAATCTCCCCGAGATCCCAGAATAATGAAGAAGGGGGAGACTATTATGATGATGAGCTGTTCTCTGATGTCCAAGATATTAAAACAGCCTTGGCCAAAATACACGAGGATAATCAGAAGATAATCTCCAAGCTAGAATCACTGCTGTTATTGAAGGGAGAAGTTGAGTCAATTAAGAAGCAGATCAACAGGCAAAATATCAGCATATCCACCCTGGAAGGACACCTCTCAAGCATCATGATCGCCATTCCTGGACTTGGGAAGGATCCCAACGACCCCACTGCAGATGTCGAAATCAATCCCGACTTGAAACCCATCATAGGCAGAGATTCAGGCCGAGCACTGGCCGAAGTTCTCAAGAAACCCGTTGCCAGCCGACAACTCCAAGGAATGACAAATGGACGGACCAGTTCCAGAGGACAGCTGCTGAAGGAATTTCAGCTAAAGCCGATCGGGAAAAAGATGAGCTCAGCCGTCGGGTTTGTTCCTGACACCGGCCCTGCATCACGCAGTGTAATCCGCTCCATTATAAAATCCAGCCGGCTAGAGGAGGATCGGAAGCGTTACCTGATGACTCTCCTTGATGATATCAAAGGAGCCAATGATCTTGCCAAGTTCCACCAGATGCTGATGAAGATAATAATGAAGTAGCTACAGCTCAACTTACCTGCCAACCCCATGCCAGTCGACCCACCTAGTACAACCTAAATCCATTATAAAAAACTTAGGAGCAAAGTGATTGCCTCCCAAGGTCCACAATGACAGAGACCTACGACTTCGACAAGTCGGCATGGGACATCAAAGGGTCGATCGCTCCGATACAACCCACCACCTACAGTGATGGCAGGCTGGTGCCCCAGGTCAGAGTCATAGATCCTGGTCTAGGCGACAGGAAGGATGAATGCTTTATGTACATGTTTCTGCTGGGGGTTGTTGAGGACAGCGATTCCCTAGGGCCTCCAATCGGGCGAGCATTTGGGTTCCTGCCCTTAGGTGTTGGCAGATCCACAGCAAAGCCCGAAAAACTCCTCAAAGAGGCCACTGAGCTTGACATAGTTGTTAGACGTACAGCAGGGCTCAATGAAAAACTGGTGTTCTACAACAACACCCCACTAACTCTCCTCACACCTTGGAGAAAGGTCCTAACAACAGGGAGTGTCTTCAACGCAAACCAAGTGTGCAATGCGGTTAATCTGATACCGCTCGATACCCCGCAGAGGTTCCGTGTTGTTTATATGAGCATCACCCGTCTTTCGGATAACGGGTATTACACCGTTCCTAGAAGAATGCTGGAATTCAGATCGGTCAATGCAGTGGCCTTCAACCTGCTGGTGACCCTTAGGATTGACAAGGCGATAGGCCCTGGGAAGATCATCGACAATACAGAGCAACTTCCTGAGGCAACATTTATGGTCCACATCGGGAACTTCAGGAGAAAGAAGAGTGAAGTCTACTCTGCCGATTATTGCAAAATGAAAATCGAAAAGATGGGCCTGGTTTTTGCACTTGGTGGGATAGGGGGCACCAGTCTTCACATTAGAAGCACAGGCAAAATGAGCAAGACTCTCCATGCACAACTCGGGTTCAAGAAGACCTTATGTTACCCGCTGATGGATATCAATGAAGACCTTAATCGATTACTCTGGAGGAGCAGATGCAAGATAGTAAGAATCCAGGCAGTTTTGCAGCCATCAGTTCCTCAAGAATTCCGCATTTACGACGACGTGATCATAAATGATGACCAAGGACTATTCAAAGTTCTGTAGACCGTAGTGCCCAGCAATGCCCGAAAACGACCCCCCTCACAATGACAGCCAGAAGGCCCGGACAAAAAAGCCCCCTCCGAAAGACTCCACGGACCAAGCGAGAGGCCAGCCAGCAGCCGACGGCAAGCGCGAACACCAGGCGGCCCCAGCACAGAACAGCCCTGACACAAGGCCACCACCAGCCACCCCAATCTGCATCCTCCTCGTGGGACCCCCGAGGACCAACCCCCAAGGCTGCCCCCGATCCAAACCACCAACCGCATCCCCACCACCCCCGGGAAAGAAACCCCCAGCAATTGGAAGGCCCCTCCCCCTCTTCCTCAACACAAGAACTCCACAACCGAACCGCACAAGCGACCGAGGTGACCCAACCGCAGGCATCCGACTCCCTAGACAGATCCTCTCTCCCCGGCAAACTAAACAAAACTTAGGGCCAAGGAACATACACACCCAACAGAACCCAGACCCCGGCCCACGGCGCCGCGCCCCCAACCCCCGACAACCAGAGGGAGCCCCCAACCAATCCCGCCGGCTCCCCCGGTGCCCACAGGCAGGGACACCAACCCCCGAACAGACCCAGCACCCAACCATCGACAATCCAAGACGGGGGGGCCCCCCCAAAAAAAGGCCCCCAGGGGCCGACAGCCAGCACCGCGAGGAAGCCCACCCACCCCACACACGACCACGGCAACCAAACCAGAACCCAGACCACCCTGGGCCACCAGCTCCCAGACTCGGCCATCACCCCGCAGAAAGGAAAGGCCACAACCCGCGCACCCCAGCCCCGATCCGGCGGGGAGCCACCCAACCCGAACCAGCACCCAAGAGCGATCCCCGAAGGACCCCCGAACCGCAAAGGACATCAGTATCCCACAGCCTCTCCAAGTCCCCCGGTCTCCTCCTCTTCTCGAAGGGACCAAAAGATCAATCCACCACACCCGACGACACTCAACTCCCCACCCCTAAAGGAGACACCGGGAATCCCAGAATCAAGACTCATCCAATGTCCATCATGGGTCTCAAGGTGAACGTCTCTGCCATATTCATGGCAGTACTGTTAACTCTCCAAACACCCACCGGTCAAATCCATTGGGGCAATCTCTCTAAGATAGGGGTGGTAGGAATAGGAAGTGCAAGCTACAAAGTTATGACTCGTTCCAGCCATCAATCATTAGTCATAAAATTAATGCCCAATATAACTCTCCTCAATAACTGCACGAGGGTAGAGATTGCAGAATACAGGAGACTACTGAGAACAGTTTTGGAACCAATTAGAGATGCACTTAATGCAATGACCCAGAATATAAGACCGGTTCAGAGTGTAGCTTCAAGTAGGAGACACAAGAGATTTGCGGGAGTAGTCCTGGCAGGTGCGGCCCTAGGCGTTGCCACAGCTGCTCAGATAACAGCCGGCATTGCACTTCACCAGTCCATGCTGAACTCTCAAGCCATCGACAATCTGAGAGCGAGCCTGGAAACTACTAATCAGGCAATTGAGACAATCAGACAAGCAGGGCAGGAGATGATATTGGCTGTTCAGGGTGTCCAAGACTACATCAATAATGAGCTGATACCGTCTATGAACCAACTATCTTGTGATTTAATCGGCCAGAAGCTCGGGCTCAAATTGCTCAGATACTATACAGAAATCCTGTCATTATTTGGCCCCAGTTTACGGGACCCCATATCTGCGGAGATATCTATCCAGGCTTTGAGCTATGCGCTTGGAGGAGACATCAATAAGGTGTTAGAAAAGCTCGGATACAGTGGAGGTGATTTACTGGGCATCTTAGAGAGCGGAGGAATAAAGGCCCGGATAACTCACGTCGACACAGAGTCCTACTTCATTGTCCTCAGTATAGCCTATCCGACGCTGTCCGAGATTAAGGGGGTGATTGTCCACCGGCTAGAGGGGGTCTCGTACAACATAGGCTCTCAAGAGTGGTATACCACTGTGCCCAAGTATGTTGCAACCCAAGGGTACCTTATCTCGAATTTTGATGAGTCATCGTGTACTTTCATGCCAGAGGGGACTGTGTGCAGCCAAAATGCCTTGTACCCGATGAGTCCTCTGCTCCAAGAATGCCTCCGGGGGTACACCAAGTCCTGTGCTCGTACACTCGTATCCGGGTCTTTTGGGAACCGGTTCATTTTATCACAAGGGAACCTAATAGCCAATTGTGCATCAATCCTTTGCAAGTGTTACACAACAGGAACGATCATTAATCAAGACCCTGACAAGATCCTAACATACATTGCTGCCGATCACTGCCCGGTAGTCGAGGTGAACGGCGTGACCATCCAAGTCGGGAGCAGGAGGTATCCAGACGCTGTGTACTTGCACAGAATTGACCTCGGTCCTCCCATATCATTGGAGAGGTTGGACGTAGGGACAAATCTGGGGAATGCAATTGCTAAGTTGGAGGATGCCAAGGAATTGTTGGAGTCATCGGACCAGATATTGAGGAGTATGAAAGGTTTATCGAGCACTAGCATAGTCTACATCCTGATTGCAGTGTGTCTTGGAGGGTTGATAGGGATCCCCGCTTTAATATGTTGCTGCAGGGGGCGTTGTAACAAAAAGGGAGAACAAGTTGGTATGTCAAGACCAGGCCTAAAGCCTGATCTTACGGGAACATCAAAATCCTATGTAAGGTCGCTCTGATCCTCTACAACTCTTGAAACACAAATGTCCCACAAGTCTCCTCTTCGTCATCAAGCAACCACCGCACCCAGCATCAAGCCCACCTGAAATTATCTCCGGCTTCCCTCTGGCCGAACAATATCGGTAGTTAATTAAAACTTAGGGTGCAAGATCATCGATAATGTCACCACAACGAGACCGGATAAATGCCTTCTACAAAGATAACCCCCATCCCAAGGGAAGTAGGATAGTCATTAACAGAGAACATCTTATGATTGATAGACCTTATGTTTTGCTGGCTGTTCTGTTTGTCATGTTTCTGAGCTTGATCGGGTTGCTAGCCATTGCAGGAATTCGACTTCATCGGGCAGCCATCTACACCGCAGAGATCCATAAAAGCCTCAGCACCAATCTAGATGTAACTAACTCAATCGAGCATCAGGTCAAGGACGTGCTGACACCACTCTTCAAAATCATCGGTGATGAAGTGGGCCTGAGGACACCTCAGAGATTCACTGACCTAGTGAAATTAATCTCTGACAAGATTAAATTCCTTAATCCGGATAGGGAGTACGACTTCAGAGATCTCACTTGGTGTATCAACCCGCCAGAGAGAATCAAATTGGATTATGATCAATACTGTGCAGATGTGGCTGCTGAAGAGCTCATGAATGCATTGGTGAACTCAACTCTACTGGAGACCAGAACAACCAATCAGTTCCTAGCTGTCTCAAAGGGAAACTGCTCAGGGCCCACTACAATCAGAGGTCAATTCTCAAACATGTCGCTGTCCCTGTTAGACTTGTATTTAGGTCGAGGTTACAATGTGTCATCTATAGTCACTATGACATCCCAGGGAATGTATGGGGGAACTTACCTAGTGGAAAAGCCTAATCTGAGCAGCAAAAGGTCAGAGTTGTCACAACTGAGCATGTACCGAGTGTTTGAAGTAGGTGTTATCAGAAATCCGGGTTTGGGGGCTCCGGTGTTCCATATGACAAACTATCTTGAGCAACCAGTCAGTAATGATCTCAGCAACTGTATGGTGGCTTTGGGGGAGCTCAAACTCGCAGCCCTTTGTCACGGGGAAGATTCTATCACAATTCCCTATCAGGGATCAGGGAAAGGTGTCAGCTTCCAGCTCGTCAAGCTAGGTGTCTGGAAATCCCCAACCGACATGCAATCCTGGGTCCCCTTATCAACGGATGATCCAGTGATAGACAGGCTTTACCTCTCATCTCACAGAGGTGTTATCGCTGACAACCAAGCAAAATGGGCTGTCCCGACAACACGAACAGATGACAAGTTGCGAATGGAGACATGCTTCCAACAGGCGTGTAAGGGTAAAATCCAAGCACTCTGCGAGAATCCCGAGTGGGCACCATTGAAGGATAACAGGATTCCTTCATACGGGGTCTTGTCTGTTGATCTGAGTCTGACAGTTGAGCTTAAAATCAAAATTGCTTCGGGATTCGGGCCATTGATCACACACGGTTCAGGGATGGACCTATACAAATCCAACCACAACAATGTGTATTGGCTGACTATCCCGCCAATGAAGAACCTAGCCTTAGGTGTAATCAACACATTGGAGTGGATACCGAGATTCAAGGTTAGTCCCTACCTCTTCACTGTCCCAATTAAGGAAGCAGGCGAAGACTGCCATGCCCCAACATACCTACCTGCGGAGGTGGATGGTGATGTCAAACTCAGTTCCAATCTGGTGATTCTACCTGGTCAAGATCTCCAATATGTTTTGGCAACCTACGATACTTCCAGGGTTGAACATGCTGTGGTTTATTACGTTTACAGCCCAAGCCGCTCATTTTCTTACTTTTATCCTTTTAGGTTGCCTATAAAGGGGGTCCCCATCGAATTACAAGTGGAATGCTTCACATGGGACCAAAAACTCTGGTGCCGTCACTTCTGTGTGCTTGCGGACTCAGAATCTGGTGGACATATCACTCACTCTGGGATGGTGGGCATGGGAGTCAGCTGCACAGTCACCCGGGAAGATGGAACCAATCGCAGATAGGGCTGCTAGTGAACCAATCACATGATGTCACCCAGACATCAGGCATACCCACTAGTGTGAAATAGACATCAGAATTAAGAAAAACGTAGGGTCCAAGTGGTTCCCCGTTATGGACTCGCTATCTGTCAACCAGATCTTATACCCTGAAGTTCACCTAGATAGCCCGATAGTTACCAATAAGATAGTAGCCATCCTGGAGTATGCTCGAGTCCCTCACGCTTACAGCCTGGAGGACCCTACACTGTGTCAGAACATCAAGCACCGCCTAAAAAACGGATTTTCCAACCAAATGATTATAAACAATGTGGAAGTTGGGAATGTCATCAAGTCCAAGCTTAGGAGTTATCCGGCCCACTCTCATATTCCATATCCAAATTGTAATCAGGATTTATTTAACATAGAAGACAAAGAGTCAACGAGGAAGATCCGTGAACTCCTCAAAAAGGGGAATTCGCTGTACTCCAAAGTCAGTGATAAGGTTTTCCAATGCTTAAGGGACACTAACTCACGGCTTGGCCTAGGCTCCGAATTGAGGGAGGACATCAAGGAGAAAGTTATTAACTTGGGAGTTTACATGCACAGCTCCCAGTGGTTTGAGCCCTTTCTGTTTTGGTTTACAGTCAAGACTGAGATGAGGTCAGTGATTAAATCACAAACCCATACTTGCCATAGGAGGAGACACACACCTGTATTCTTCACTGGTAGTTCAGTTGAGTTGCTAATCTCTCGTGACCTTGTTGCTATAATCAGTAAAGAGTCTCAACATGTATATTACCTGACATTTGAACTGGTTTTGATGTATTGTGATGTCATAGAGGGGAGGTTAATGACAGAGACCGCTATGACTATTGATGCTAGGTATACAGAGCTTCTAGGAAGAGTCAGATACATGTGGAAACTGATAGATGGTTTCTTCCCTGCACTCGGGAATCCAACTTATCAAATTGTAGCCATGCTGGAGCCTCTTTCACTTGCTTACCTGCAGCTGAGGGATATAACAGTAGAACTCAGAGGTGCTTTCCTTAACCACTGCTTTACTGAAATACATGATGTTCTTGACCAAAACGGGTTTTCTGATGAAGGTACTTATCATGAGTTAACTGAAGCTCTAGATTACATTTTCATAACTGATGACATACATCTGACAGGGGAGATTTTCTCATTTTTCAGAAGTTTCGGCCACCCCAGACTTGAAGCAGTAACGGCTGCTGAAAATGTTAGGAAATACATGAATCAGCCTAAAGTCATTGTGTATGAGACTCTGATGAAAGGTCATGCCATATTTTGTGGAATCATAATCAACGGCTATCGTGACAGGCACGGAGGCAGTTGGCCACCGCTGACCCTCCCCCTGCATGCTGCAGACACAATCCGGAATGCTCAAGCTTCAGGTGAAGGGTTAACACATGAGCAGTGCGTTGATAACTGGAAATCTTTTGCTGGAGTGAAATTTGGCTGCTTTATGCCTCTTAGCCTGGATAGTGATCTGACAATGTACCTAAAGGACAAGGCACTTGCTGCTCTCCAAAGGGAATGGGATTCAGTTTACCCGAAAGAGTTCCTGCGTTACGACCCTCCCAAGGGAACCGGGTCACGGAGGCTTGTAGATGTTTTCCTTAATGATTCGAGCTTTGACCCATATGATGTGATAATGTATGTTGTAAGTGGAGCTTACCTCCATGACCCTGAGTTCAACCTGTCTTACAGCCTGAAAGAAAAGGAGATCAAGGAAACAGGTAGACTTTTTGCTAAAATGACTTACAAAATGAGGGCATGCCAAGTGATTGCTGAAAATCTAATCTCAAACGGGATTGGCAAATATTTTAAGGACAATGGGATGGCCAAGGATGAGCACGATTTGACTAAGGCACTCCACACTCTAGCTGTCTCAGGAGTCCCCAAAGATCTCAAAGAAAGTCACAGGGGGGGGCCAGTCTTAAAAACCTACTCCCGAAGCCCAGTCCACACAAGTACCAGGAACGTGAGAGCAGCAAAAGGGTTTATAGGGTTCCCTCAAGTAATTCGGCAGGACCAAGACACTGATCATCCGGAGAATATGGAAGCTTACGAGACAGTCAGTGCATTTATCACGACTGATCTCAAGAAGTACTGCCTTAATTGGAGATATGAGACCATCAGCTTGTTTGCACAGAGGCTAAATGAGATTTACGGATTGCCCTCATTTTTCCAGTGGCTGCATAAGAGGCTTGAGACCTCTGTCCTGTATGTAAGTGACCCTCATTGCCCCCCCGACCTTGACGCCCATATCCCGTTATATAAAGTCCCCAATGATCAAATCTTCATTAAGTACCCTATGGGAGGTATAGAAGGGTATTGTCAGAAGCTGTGGACCATCAGCACCATTCCCTATCTATACCTGGCTGCTTATGAGAGCGGAGTAAGGATTGCTTCGTTAGTGCAAGGGGACAATCAGACCATAGCCGTAACAAAAAGGGTACCCAGCACATGGCCCTACAACCTTAAGAAACGGGAAGCTGCTAGAGTAACTAGAGATTACTTTGTAATTCTTAGGCAAAGGCTACATGATATTGGCCATCACCTCAAGGCAAATGAGACAATTGTTTCATCACATTTTTTTGTCTATTCAAAAGGAATATATTATGATGGGCTACTTGTGTCCCAATCACTCAAGAGCATCGCAAGATGTGTATTCTGGTCAGAGACTATAGTTGATGAAACAAGGGCAGCATGCAGTAATATTGCTACAACAATGGCTAAAAGCATCGAGAGAGGTTATGACCGTTACCTTGCATATTCCCTGAACGTCCTAAAAGTGATACAGCAAATTCTGATCTCTCTTGGCTTCACAATCAATTCAACCATGACCCGGGATGTAGTCATACCCCTCCTCACAAACAACGACCTCTTAATAAGGATGGCACTGTTGCCCGCTCCTATTGGGGGGATGAATTATCTGAATATGAGCAGGCTGTTTGTCAGAAACATCGGTGATCCAGTAACATCATCAATTGCTGATCTCAAGAGAATGATTCTCGCCTCACTAATGCCTGAAGAGACCCTCCATCAGGTAATGACACAACAACCGGGGGACTCTTCATTCCTAGACTGGGCTAGCGACCCTTACTCAGCAAATCTTGTATGTGTCCAGAGCATCACTAGACTCCTCAAGAACATAACTGCAAGGTTTGTCCTGATCCATAGTCCAAACCCAATGTTAAAAGGATTATTCCATGATGACAGTAAAGAAGAGGACGAGGGACTGGCGGCATTCCTCATGGACAGGCATATTATAGTACCTAGGGCAGCTCATGAAATCCTGGATCATAGTGTCACAGGGGCAAGAGAGTCTATTGCAGGCATGCTGGATACCACAAAAGGCTTGATTCGAGCCAGCATGAGGAAGGGGGGTTTAACCTCTCGAGTGATAACCAGATTGTCCAATTATGACTATGAACAATTCAGAGCAGGGATGGTGCTATTGACAGGAAGAAAGAGAAATGTCCTCATTGACAAAGAGTCATGTTCAGTGCAGCTGGCGAGAGCTCTAAGAAGCCATATGTGGGCGAGGCTAGCTCGAGGACGGCCTATTTACGGCCTTGAGGTCCCTGATGTACTAGAATCTATGCGAGGCCACCTTATTCGGCGTCATGAGACATGTGTCATCTGCGAGTGTGGATCAGTCAACTACGGATGGTTTTTTGTCCCCTCGGGTTGCCAACTGGATGATATTGACAAGGAAACATCATCCTTGAGAGTCCCATATATTGGTTCTACCACTGATGAGAGAACAGACATGAAGCTTGCCTTCGTAAGAGCCCCAAGTCGATCCTTGCGATCTGCTGTTAGAATAGCAACAGTGTACTCATGGGCTTACGGTGATGATGATAGCTCTTGGAACGAAGCCTGGTTGTTGGCTAGGCAAAGGGCCAATGTGAGCCTGGAGGAGCTAAGGGTGATCACTCCCATCTCAACTTCGACTAATTTAGCGCATAGGTTGAGGGATCGTAGCACTCAAGTGAAATACTCAGGTACATCCCTTGTCCGAGTGGCGAGGTATACCACAATCTCCAACGACAATCTCTCATTTGTCATATCAGATAAGAAGGTTGATACTAACTTTATATACCAACAAGGAATGCTTCTAGGGTTGGGTGTTTTAGAAACATTGTTTCGACTCGAGAAAGATACCGGATCATCTAACACGGTATTACATCTTCACGTCGAAACAGATTGTTGCGTGATCCCGATGATAGATCATCCCAGGATACCCAGCTCCCGCAAGCTAGAGCTGAGGGCAGAGCTATGTACCAACCCATTGATATATGATAATGCACCTTTAATTGACAGAGATGCAACAAGGCTATACACCCAGAGCCATAGGAGGCACCTTGTGGAATTTGTTACATGGTCCACACCCCAACTATATCACATTTTAGCTAAGTCCACAGCACTATCTATGATTGACCTGGTAACAAAATTTGAGAAGGACCATATGAATGAAATTTCAGCTCTCATAGGGGATGACGATATCAATAGTTTCATAACTGAGTTTCTGCTCATAGAGCCAAGATTATTCACTATCTACTTGGGCCAGTGTGCGGCCATCAATTGGGCATTTGATGTACATTATCATAGACCATCAGGGAAATATCAGATGGGTGAGCTGTTGTCATCGTTCCTTTCTAGAATGAGCAAAGGAGTGTTTAAGGTGCTTGTCAATGCTCTAAGCCACCCAAAGATCTACAAGAAATTCTGGCATTGTGGTATTATAGAGCCTATCCATGGTCCTTCACTTGATGCTCAAAACTTGCACACAACTGTGTGCAACATGGTTTACACATGCTATATGACCTACCTCGACCTGTTGTTGAATGAAGAGTTAGAAGAGTTCACATTTCTCTTGTGTGAAAGCGACGAGGATGTAGTACCGGACAGATTCGACAACATCCAGGCAAAACACTTATGTGTTCTGGCAGATTTGTACTGTCAACCAGGGACCTGCCCACCAATTCGAGGTCTAAGACCGGTAGAGAAATGTGCAGTTCTAACCGACCATATCAAGGCAGAGGCTATGTTATCTCCAGCAGGATCTTCGTGGAACATAAATCCAATTATTGTAGACCATTACTCATGCTCCCTGACTTATCTCCGGCGAGGATCGATCAAACAGATAAGATTGAGAGTTGATCCAGGATTCATTTTCGACGCCCTCGCTGAGGTAAATGTCAGTCAGCCAAAGATCGGCAGCAACAACATCTCAAATATGAGCATCAAGGCTTTCAGACCCCCACACGATGATGTTGCAAAATTGCTCAAAGATATCAACACAAGCAAGCACAATCTTCCCATTTCAGGGGGCAATCTCGCCAATTATGAAATCCATGCTTTCCGCAGAATCGGGTTGAACTCATCTGCTTGCTACAAAGCTGTTGAGATATCAACATTAATTAGGAGATGCCTTGAGCCAGGGGAGGACGGCTTGTTCTTGGGTGAGGGATCGGGTTCTATGTTGATCACTTATAAGGAGATACTTAAACTAAACAAGTGCTTCTATAATAGTGGGGTTTCCGCCAATTCTAGATCTGGTCAAAGGGAATTAGCACCCTATCCCTCCGAAGTTGGCCTTGTCGAACACAGAATGGGAGTAGGTAATATTGTCAAAGTGCTCTTTAACGGGAGGCCCGAAGTCACGTGGGTAGGCAGTGTAGATTGCTTCAATTTCATAGTTAGTAATATCCCTACCTCTAGTGTGGGGTTTATCCATTCAGATATAGAGACCTTGCCTGACAAAGATACTATAGAGAAGCTAGAGGAATTGGCAGCCATCTTATCGATGGCTCTGCTCCTGGGCAAAATAGGATCAATACTGGTGATTAAGCTTATGCCTTTCAGCGGGGATTTTGTTCAGGGATTTATAAGTTATGTAGGGTCTCATTATAGAGAAGTGAACCTTGTATACCCTAGATACAGCAACTTCATATCTACTGAATCTTATTTGGTTATGACAGATCTCAAGGCTAACCGGCTAATGAATCCTGAAAAGATTAAGCAGCAGATAATTGAATCATCTGTGAGGACTTCACCTGGACTTATAGGTCACATCCTATCCATTAAGCAACTAAGCTGCATACAAGCAATTGTGGGAGACGCAGTTAGTAGAGGTGATATCAATCCTACTCTGAAAAAACTTACACCTATAGAGCAGGTGCTGATCAATTGCGGGTTGGCAATTAACGGACCTAAGCTGTGCAAAGAATTGATCCACCATGATGTTGCCTCAGGGCAAGATGGATTGCTTAATTCTATACTCATCCTCTACAGGGAGTTGGCAAGATTCAAAGACAACCAAAGAAGTCAACAAGGGATGTTCCACGCCTACCCCGTATTGGTAAGTAGCAGGCAACGAGAACTTATATCTAGGATCACCCGCAAATTTTGGGGGCACATTCTTCTTTACTCCGGGAACAAAAAGTTGATAAATAAGTTTATCCAGAATCTCAAGTCCGGCTATCTGATACTAGACTTACACCAGAATATCTTCGTTAAGAATCTATCCAAGTCAGAGAAACAGATTATTATGACGGGGGGTTTGAAACGTGAGTGGGTTTTTAAGGTAACAGTCAAGGAGACCAAAGAATGGTATAAGTTAGTCGGATACAGTGCCCTGATTAAGGACTAATTGGTTGAACTCCGGAACCCTAATCCTGCCCTAGGTGGTTAGGCATTATTTGCAATATATTAAAGAAAACTTTGAAAATACGAAGTTTCTATTCCCAGCTTTGTCTGGTggccggcatAgtcccagcctcctcgctggcgctggctgggcaacattccgaggggaccgtccccAcggtaatggcgaatgggacgcggccgatccggctgctaacaaagcccgaaaggaagctgagttggctgctgGcGcTGgctgGgcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggatgcGGCCGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTG。
在另一个实施方式中，核酸包括SEQ ID NO：4的序列，如下所示：
GGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGCGCGCCGTTAATACGACTCACTATAGGGAGACCCAAGCTGGCTAGCTTTGTTTGGTCTGATGAGTCCCGTGAGGACGAAACCCGGCGTACCGGGTCACCAAACAAAGTTGGGTAAGGATAGTTCAATCAATGATCATCTTCTAGTGCACTTAGGATTCAAGATCCTATTATCAGGGACAAGAGCAGGATTAGGGATATCCGAGATGGCCACACTTTTAAGGAGCTTAGCATTGTTCAAAAGAAACAAGGACAAACCACCCATTACATCAGGATCCGGTGGAGCCATCAGAGGAATCAAACACATTATTATAGTACCAATCCCTGGAGATTCCTCAATTACCACTCGATCCAGACTTCTGGACCGGTTGGTGAGGTTAATTGGAAACCCGGATGTGAGCGGGCCCAAACTAACAGGGGCACTAATAGGTATATTATCCTTATTTGTGGAGTCTCCAGGTCAATTGATTCAGAGGATCACCGATGACCCTGACGTTAGCATAAGGCTGTTAGAGGTTGTCCAGAGTGACCAGTCACAATCTGGCCTTACCTTCGCATCAAGAGGTACCAACATGGAGGATGAGGCGGACCAATACTTTTCACATGATGATCCAATTAGTAGTGATCAATCCAGGTTCGGATGGTTCGGGAACAAGGAAATCTCAGATATTGAAGTGCAAGACCCTGAGGGATTCAACATGATTCTGGGTACCATCCTAGCCCAAATTTGGGTCTTGCTCGCAAAGGCGGTTACGGCCCCAGACACGGCAGCTGATTCGGAGCTAAGAAGGTGGATAAAGTACACCCAACAAAGAAGGGTAGTTGGTGAATTTAGATTGGAGAGAAAATGGTTGGATGTGGTGAGGAACAGGATTGCCGAGGACCTCTCCTTACGCCGATTCATGGTCGCTCTAATCCTGGATATCAAGAGAACACCCGGAAACAAACCCAGGATTGCTGAAATGATATGTGACATTGATACATATATCGTAGAGGCAGGATTAGCCAGTTTTATCCTGACTATTAAGTTTGGGATAGAAACTATGTATCCTGCTCTTGGACTGCATGAATTTGCTGGTGAGTTATCCACACTTGAGTCCTTGATGAACCTTTACCAGCAAATGGGGGAAACTGCACCCTACATGGTAATCCTGGAGAACTCAATTCAGAACAAGTTCAGTGCAGGATCATACCCTCTGCTCTGGAGCTATGCCATGGGAGTAGGAGTGGAACTTGAAAACTCCATGGGAGGTTTGAACTTTGGCCGATCTTACTTTGATCCAGCATATTTTAGATTAGGGCAAGAGATGGTAAGGAGGTCAGCTGGAAAGGTCAGTTCCACATTGGCATCTGAACTCGGTATCACTGCCGAGGATGCAAGGCTTGTTTCAGAGATTGCAATGCATACTACTGAGGACAAGATCAGTAGAGCGGTTGGACCCAGACAAGCCCAAGTATCATTTCTACACGGTGATCAAAGTGAGAATGAGCTACCGAGATTGGGGGGCAAGGAAGATAGGAGGGTCAAACAGAGTCGAGGAGAAGCCAGGGAGAGCTACAGAGAAACCGGGCCCAGCAGAGCAAGTGATGCGAGAGCTGCCCATCTTCCAACCGGCACACCCCTAGACATTGACACTGCAACGGAGTCCAGCCAAGATCCGCAGGACAGTCGAAGGTCAGCTGACGCCCTGCTTAGGCTGCAAGCCATGGCAGGAATCTCGGAAGAACAAGGCTCAGACACGGACACCCCTATAGTGTACAATGACAGAAATCTTCTAGACTAGGTGCGAGAGGCCGAGGGCCAGAACAACATCCGCCTACCATCCATCATTGTTATAAAAAACTTAGGAACCAGGTCCACACAGCCGCCAGCCCATCAACCATCCACTCCCACGATTGGAGCCAATGGCAGAAGAGCAGGCACGCCATGTCAAAAACGGACTGGAATGCATCCGGGCTCTCAAGGCCGAGCCCATCGGCTCACTGGCCATCGAGGAAGCTATGGCAGCATGGTCAGAAATATCAGACAACCCAGGACAGGAGCGAGCCACCTGCAGGGAAGAGAAGGCAGGCAGTTCGGGTCTCAGCAAACCATGCCTCTCAGCAATTGGATCAACTGAAGGCGGTGCACCTCGCATCCGCGGTCAGGGACCTGGAGAGAGCGATGACGACGCTGAAACTTTGGGAATCCCCCCAAGAAATCTCCAGGCATCAAGCACTGGGTTACAGTGTTATTACGTTTATGATCACAGCGGTGAAGCGGTTAAGGGAATCCAAGATGCTGACTCTATCATGGTTCAATCAGGCCTTGATGGTGATAGCACCCTCTCAGGAGGAGACAATGAATCTGAAAACAGCGATGTGGATATTGGCGAACCTGATACCGAGGGATATGCTATCACTGACCGGGGATCTGCTCCCATCTCTATGGGGTTCAGGGCTTCTGATGTTGAAACTGCAGAAGGAGGGGAGATCCACGAGCTCCTGAGACTCCAATCCAGAGGCAACAACTTTCCGAAGCTTGGGAAAACTCTCAATGTTCCTCCGCCCCCGGACCCCGGTAGGGCCAGCACTTCCGGGACACCCATTAAAAAGGGCACAGACGCGAGATTAGCCTCATTTGGAACGGAGATCGCGTCTTTATTGACAGGTGGTGCAACCCAATGTGCTCGAAAGTCACCCTCGGAACCATCAGGGCCAGGTGCACCTGCGGGGAATGTCCCCGAGTGTGTGAGCAATGCCGCACTGATACAGGAGTGGACACCCGAATCTGGTACCACAATCTCCCCGAGATCCCAGAATAATGAAGAAGGGGGAGACTATTATGATGATGAGCTGTTCTCTGATGTCCAAGATATTAAAACAGCCTTGGCCAAAATACACGAGGATAATCAGAAGATAATCTCCAAGCTAGAATCACTGCTGTTATTGAAGGGAGAAGTTGAGTCAATTAAGAAGCAGATCAACAGGCAAAATATCAGCATATCCACCCTGGAAGGACACCTCTCAAGCATCATGATCGCCATTCCTGGACTTGGGAAGGATCCCAACGACCCCACTGCAGATGTCGAAATCAATCCCGACTTGAAACCCATCATAGGCAGAGATTCAGGCCGAGCACTGGCCGAAGTTCTCAAGAAACCCGTTGCCAGCCGACAACTCCAAGGAATGACAAATGGACGGACCAGTTCCAGAGGACAGCTGCTGAAGGAATTTCAGCTAAAGCCGATCGGGAAAAAGATGAGCTCAGCCGTCGGGTTTGTTCCTGACACCGGCCCTGCATCACGCAGTGTAATCCGCTCCATTATAAAATCCAGCCGGCTAGAGGAGGATCGGAAGCGTTACCTGATGACTCTCCTTGATGATATCAAAGGAGCCAATGATCTTGCCAAGTTCCACCAGATGCTGATGAAGATAATAATGAAGTAGCTACAGCTCAACTTACCTGCCAACCCCATGCCAGTCGATCATCCATCATTGTTATAAAAAACTTAGGAACCAggtccacacaGagtgatACGCGTACGCCACCATGTTCGTGTTTCTGGTGCTGCTGCCTCTGGTGAGCTCCCAGTGCGTGAACCTGACCACAAGGACCCAGCTGCCCCCTGCCTATACCAATTCCTTCACACGGGGCGTGTACTATCCCGACAAGGTGTTCCGGAGCAGCGTGCTGCACTCCACACAGGATCTGTTTCTGCCTTTCTTTTCTAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAATGGCACAAAGCGGTTCGACAATCCAGTGCTGCCCTTTAACGATGGCGTGTACTTCGCCTCCACCGAGAAGTCTAACATCATCAGAGGCTGGATCTTTGGCACCACACTGGACAGCAAGACACAGTCCCTGCTGATCGTGAACAATGCCACCAACGTGGTCATCAAGGTGTGCGAGTTCCAGTTTTGTAATGATCCATTCCTGGGCGTGTACTATCACAAGAACAATAAGTCTTGGATGGAGAGCGAGTTTCGCGTGTATTCCTCTGCCAACAATTGCACATTTGAGTACGTGTCCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAATTTCAAGAACCTGAGGGAGTTCGTGTTTAAGAATATCGATGGCTACTTCAAAATCTACTCCAAGCACACCCCAATCAACCTGGTGCGCGACCTGCCACAGGGCTTCTCTGCCCTGGAGCCACTGGTGGATCTGCCCATCGGCATCAACATCACCCGGTTTCAGACACTGCTGGCCCTGCACAGAAGCTACCTGACACCAGGCGACAGCTCCTCTGGATGGACCGCAGGAGCAGCAGCCTACTATGTGGGCTATCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAATGGCACCATCACAGACGCCGTGGATTGCGCCCTGGATCCCCTGTCTGAGACCAAGTGTACACTGAAGAGCTTTACCGTGGAGAAGGGCATCTATCAGACAAGCAATTTCAGGGTGCAGCCTACCGAGTCCATCGTGCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAAGATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGGAGGGCTTTAACTGTTATTTCCCTCTGCAGAGCTACGGCTTCCAGCCAACAAACGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACCTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGACTGACCGGAACAGGCGTGCTGACCGAGTCCAACAAGAAGTTCCTGCCTTTTCAGCAGTTCGGCAGGGACATCGCAGATACCACAGACGCCGTGCGCGACCCTCAGACCCTGGAGATCCTGGACATCACACCATGCTCCTTCGGCGGCGTGTCTGTGATCACACCAGGCACCAATACAAGCAACCAGGTGGCCGTGCTGTATCAGGACGTGAATTGTACCGAGGTGCCAGTGGCAATCCACGCAGATCAGCTGACCCCTACATGGCGGGTGTACTCTACCGGCAGCAACGTGTTCCAGACAAGAGCCGGATGCCTGATCGGAGCAGAGCACGTGAACAATAGCTATGAGTGCGACATCCCTATCGGCGCCGGCATCTGTGCCTCCTACCAGACCCAGACAAACTCCCCAAGGAGAGCACGGTCTGTGGCCAGCCAGTCCATCATCGCCTATACCATGAGCCTGGGCGCCGAGAATTCCGTGGCCTACTCCAACAATTCTATCGCCATCCCTACCAACTTCACAATCTCCGTGACCACAGAGATCCTGCCAGTGAGCATGACCAAGACATCCGTGGACTGCACAATGTATATCTGTGGCGATTCCACCGAGTGCTCTAACCTGCTGCTGCAGTACGGCTCTTTTTGTACCCAGCTGAATAGAGCCCTGACAGGCATCGCCGTGGAGCAGGACAAGAACACACAGGAGGTGTTCGCCCAGGTGAAGCAAATCTACAAGACCCCACCCATCAAGGACTTTGGCGGCTTCAACTTCAGCCAGATCCTGCCCGATCCTAGCAAGCCATCCAAGCGGTCTTTTATCGAGGACCTGCTGTTCAACAAGGTGACCCTGGCCGATGCCGGCTTCATCAAGCAGTATGGCGATTGCCTGGGCGACATCGCCGCCAGAGACCTGATCTGTGCCCAGAAGTTTAATGGCCTGACCGTGCTGCCTCCACTGCTGACAGATGAGATGATCGCCCAGTACACATCTGCCCTGCTGGCCGGAACCATCACAAGCGGATGGACCTTCGGCGCAGGAGCCGCCCTGCAGATCCCCTTTGCCATGCAGATGGCCTATCGGTTCAACGGCATCGGCGTGACCCAGAATGTGCTGTACGAGAACCAGAAGCTGATCGCCAATCAGTTTAACTCCGCCATCGGCAAGATCCAGGACTCTCTGAGCTCCACAGCCAGCGCCCTGGGCAAGCTGCAGGATGTGGTGAATCAGAACGCCCAGGCCCTGAATACCCTGGTGAAGCAGCTGTCTAGCAACTTCGGCGCCATCTCCTCTGTGCTGAATGACATCCTGAGCCGGCTGGACAAGGTGGAGGCAGAGGTGCAGATCGACCGGCTGATCACAGGCAGACTGCAGTCCCTGCAGACCTACGTGACACAGCAGCTGATCAGGGCAGCAGAGATCAGGGCCTCTGCCAATCTGGCCGCCACCAAGATGAGCGAGTGCGTGCTGGGCCAGTCCAAGAGAGTGGACTTTTGTGGCAAGGGCTATCACCTGATGAGCTTCCCACAGTCCGCCCCTCACGGAGTGGTGTTTCTGCACGTGACCTACGTGCCAGCCCAGGAGAAGAACTTCACCACAGCACCAGCAATCTGCCACGATGGCAAGGCACACTTTCCTAGGGAGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTTGTGACACAGCGCAATTTCTACGAGCCACAGATCATCACCACAGACAATACATTCGTGTCCGGCAACTGTGACGTGGTCATCGGCATCGTGAACAATACCGTGTATGATCCTCTGCAGCCAGAGCTGGACTCTTTTAAGGAGGAGCTGGATAAGTACTTCAAGAATCACACCAGCCCCGACGTGGATCTGGGCGACATCTCTGGCATCAATGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAGGTGGCCAAGAATCTGAACGAGTCCCTGATCGATCTGCAGGAGCTGGGCAAGTATGAGCAGTACATCAAGTGGCCCTGGTATATCTGGCTGGGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTGTATGACAAGCTGCTGTTCCTGCCTGAAGGGCTGCTGTTCTTGTGGCAGCTGCTGTAAGTTTGATGAGGACGATAGCGAGCCTGTGCTGAAGGGCGTGAAGCTGCACTACACCTGATAGCTAGCGATCGCCCACCTAGTACAACCTAAATCCATTATAAAAAACTTAGGAGCAAAGTGATTGCCTCCCAAGGTCCACAATGACAGAGACCTACGACTTCGACAAGTCGGCATGGGACATCAAAGGGTCGATCGCTCCGATACAACCCACCACCTACAGTGATGGCAGGCTGGTGCCCCAGGTCAGAGTCATAGATCCTGGTCTAGGCGACAGGAAGGATGAATGCTTTATGTACATGTTTCTGCTGGGGGTTGTTGAGGACAGCGATTCCCTAGGGCCTCCAATCGGGCGAGCATTTGGGTTCCTGCCCTTAGGTGTTGGCAGATCCACAGCAAAGCCCGAAAAACTCCTCAAAGAGGCCACTGAGCTTGACATAGTTGTTAGACGTACAGCAGGGCTCAATGAAAAACTGGTGTTCTACAACAACACCCCACTAACTCTCCTCACACCTTGGAGAAAGGTCCTAACAACAGGGAGTGTCTTCAACGCAAACCAAGTGTGCAATGCGGTTAATCTGATACCGCTCGATACCCCGCAGAGGTTCCGTGTTGTTTATATGAGCATCACCCGTCTTTCGGATAACGGGTATTACACCGTTCCTAGAAGAATGCTGGAATTCAGATCGGTCAATGCAGTGGCCTTCAACCTGCTGGTGACCCTTAGGATTGACAAGGCGATAGGCCCTGGGAAGATCATCGACAATACAGAGCAACTTCCTGAGGCAACATTTATGGTCCACATCGGGAACTTCAGGAGAAAGAAGAGTGAAGTCTACTCTGCCGATTATTGCAAAATGAAAATCGAAAAGATGGGCCTGGTTTTTGCACTTGGTGGGATAGGGGGCACCAGTCTTCACATTAGAAGCACAGGCAAAATGAGCAAGACTCTCCATGCACAACTCGGGTTCAAGAAGACCTTATGTTACCCGCTGATGGATATCAATGAAGACCTTAATCGATTACTCTGGAGGAGCAGATGCAAGATAGTAAGAATCCAGGCAGTTTTGCAGCCATCAGTTCCTCAAGAATTCCGCATTTACGACGACGTGATCATAAATGATGACCAAGGACTATTCAAAGTTCTGTAGACCGTAGTGCCCAGCAATGCCCGAAAACGACCCCCCTCACAATGACAGCCAGAAGGCCCGGACAAAAAAGCCCCCTCCGAAAGACTCCACGGACCAAGCGAGAGGCCAGCCAGCAGCCGACGGCAAGCGCGAACACCAGGCGGCCCCAGCACAGAACAGCCCTGACACAAGGCCACCACCAGCCACCCCAATCTGCATCCTCCTCGTGGGACCCCCGAGGACCAACCCCCAAGGCTGCCCCCGATCCAAACCACCAACCGCATCCCCACCACCCCCGGGAAAGAAACCCCCAGCAATTGGAAGGCCCCTCCCCCTCTTCCTCAACACAAGAACTCCACAACCGAACCGCACAAGCGACCGAGGTGACCCAACCGCAGGCATCCGACTCCCTAGACAGATCCTCTCTCCCCGGCAAACTAAACAAAACTTAGGGCCAAGGAACATACACACCCAACAGAACCCAGACCCCGGCCCACGGCGCCGCGCCCCCAACCCCCGACAACCAGAGGGAGCCCCCAACCAATCCCGCCGGCTCCCCCGGTGCCCACAGGCAGGGACACCAACCCCCGAACAGACCCAGCACCCAACCATCGACAATCCAAGACGGGGGGGCCCCCCCAAAAAAAGGCCCCCAGGGGCCGACAGCCAGCACCGCGAGGAAGCCCACCCACCCCACACACGACCACGGCAACCAAACCAGAACCCAGACCACCCTGGGCCACCAGCTCCCAGACTCGGCCATCACCCCGCAGAAAGGAAAGGCCACAACCCGCGCACCCCAGCCCCGATCCGGCGGGGAGCCACCCAACCCGAACCAGCACCCAAGAGCGATCCCCGAAGGACCCCCGAACCGCAAAGGACATCAGTATCCCACAGCCTCTCCAAGTCCCCCGGTCTCCTCCTCTTCTCGAAGGGACCAAAAGATCAATCCACCACACCCGACGACACTCAACTCCCCACCCCTAAAGGAGACACCGGGAATCCCAGAATCAAGACTCATCCAATGTCCATCATGGGTCTCAAGGTGAACGTCTCTGCCATATTCATGGCAGTACTGTTAACTCTCCAAACACCCACCGGTCAAATCCATTGGGGCAATCTCTCTAAGATAGGGGTGGTAGGAATAGGAAGTGCAAGCTACAAAGTTATGACTCGTTCCAGCCATCAATCATTAGTCATAAAATTAATGCCCAATATAACTCTCCTCAATAACTGCACGAGGGTAGAGATTGCAGAATACAGGAGACTACTGAGAACAGTTTTGGAACCAATTAGAGATGCACTTAATGCAATGACCCAGAATATAAGACCGGTTCAGAGTGTAGCTTCAAGTAGGAGACACAAGAGATTTGCGGGAGTAGTCCTGGCAGGTGCGGCCCTAGGCGTTGCCACAGCTGCTCAGATAACAGCCGGCATTGCACTTCACCAGTCCATGCTGAACTCTCAAGCCATCGACAATCTGAGAGCGAGCCTGGAAACTACTAATCAGGCAATTGAGACAATCAGACAAGCAGGGCAGGAGATGATATTGGCTGTTCAGGGTGTCCAAGACTACATCAATAATGAGCTGATACCGTCTATGAACCAACTATCTTGTGATTTAATCGGCCAGAAGCTCGGGCTCAAATTGCTCAGATACTATACAGAAATCCTGTCATTATTTGGCCCCAGTTTACGGGACCCCATATCTGCGGAGATATCTATCCAGGCTTTGAGCTATGCGCTTGGAGGAGACATCAATAAGGTGTTAGAAAAGCTCGGATACAGTGGAGGTGATTTACTGGGCATCTTAGAGAGCGGAGGAATAAAGGCCCGGATAACTCACGTCGACACAGAGTCCTACTTCATTGTCCTCAGTATAGCCTATCCGACGCTGTCCGAGATTAAGGGGGTGATTGTCCACCGGCTAGAGGGGGTCTCGTACAACATAGGCTCTCAAGAGTGGTATACCACTGTGCCCAAGTATGTTGCAACCCAAGGGTACCTTATCTCGAATTTTGATGAGTCATCGTGTACTTTCATGCCAGAGGGGACTGTGTGCAGCCAAAATGCCTTGTACCCGATGAGTCCTCTGCTCCAAGAATGCCTCCGGGGGTACACCAAGTCCTGTGCTCGTACACTCGTATCCGGGTCTTTTGGGAACCGGTTCATTTTATCACAAGGGAACCTAATAGCCAATTGTGCATCAATCCTTTGCAAGTGTTACACAACAGGAACGATCATTAATCAAGACCCTGACAAGATCCTAACATACATTGCTGCCGATCACTGCCCGGTAGTCGAGGTGAACGGCGTGACCATCCAAGTCGGGAGCAGGAGGTATCCAGACGCTGTGTACTTGCACAGAATTGACCTCGGTCCTCCCATATCATTGGAGAGGTTGGACGTAGGGACAAATCTGGGGAATGCAATTGCTAAGTTGGAGGATGCCAAGGAATTGTTGGAGTCATCGGACCAGATATTGAGGAGTATGAAAGGTTTATCGAGCACTAGCATAGTCTACATCCTGATTGCAGTGTGTCTTGGAGGGTTGATAGGGATCCCCGCTTTAATATGTTGCTGCAGGGGGCGTTGTAACAAAAAGGGAGAACAAGTTGGTATGTCAAGACCAGGCCTAAAGCCTGATCTTACGGGAACATCAAAATCCTATGTAAGGTCGCTCTGATCCTCTACAACTCTTGAAACACAAATGTCCCACAAGTCTCCTCTTCGTCATCAAGCAACCACCGCACCCAGCATCAAGCCCACCTGAAATTATCTCCGGCTTCCCTCTGGCCGAACAATATCGGTAGTTAATTAAAACTTAGGGTGCAAGATCATCGATAATGTCACCACAACGAGACCGGATAAATGCCTTCTACAAAGATAACCCCCATCCCAAGGGAAGTAGGATAGTCATTAACAGAGAACATCTTATGATTGATAGACCTTATGTTTTGCTGGCTGTTCTGTTTGTCATGTTTCTGAGCTTGATCGGGTTGCTAGCCATTGCAGGAATTCGACTTCATCGGGCAGCCATCTACACCGCAGAGATCCATAAAAGCCTCAGCACCAATCTAGATGTAACTAACTCAATCGAGCATCAGGTCAAGGACGTGCTGACACCACTCTTCAAAATCATCGGTGATGAAGTGGGCCTGAGGACACCTCAGAGATTCACTGACCTAGTGAAATTAATCTCTGACAAGATTAAATTCCTTAATCCGGATAGGGAGTACGACTTCAGAGATCTCACTTGGTGTATCAACCCGCCAGAGAGAATCAAATTGGATTATGATCAATACTGTGCAGATGTGGCTGCTGAAGAGCTCATGAATGCATTGGTGAACTCAACTCTACTGGAGACCAGAACAACCAATCAGTTCCTAGCTGTCTCAAAGGGAAACTGCTCAGGGCCCACTACAATCAGAGGTCAATTCTCAAACATGTCGCTGTCCCTGTTAGACTTGTATTTAGGTCGAGGTTACAATGTGTCATCTATAGTCACTATGACATCCCAGGGAATGTATGGGGGAACTTACCTAGTGGAAAAGCCTAATCTGAGCAGCAAAAGGTCAGAGTTGTCACAACTGAGCATGTACCGAGTGTTTGAAGTAGGTGTTATCAGAAATCCGGGTTTGGGGGCTCCGGTGTTCCATATGACAAACTATCTTGAGCAACCAGTCAGTAATGATCTCAGCAACTGTATGGTGGCTTTGGGGGAGCTCAAACTCGCAGCCCTTTGTCACGGGGAAGATTCTATCACAATTCCCTATCAGGGATCAGGGAAAGGTGTCAGCTTCCAGCTCGTCAAGCTAGGTGTCTGGAAATCCCCAACCGACATGCAATCCTGGGTCCCCTTATCAACGGATGATCCAGTGATAGACAGGCTTTACCTCTCATCTCACAGAGGTGTTATCGCTGACAACCAAGCAAAATGGGCTGTCCCGACAACACGAACAGATGACAAGTTGCGAATGGAGACATGCTTCCAACAGGCGTGTAAGGGTAAAATCCAAGCACTCTGCGAGAATCCCGAGTGGGCACCATTGAAGGATAACAGGATTCCTTCATACGGGGTCTTGTCTGTTGATCTGAGTCTGACAGTTGAGCTTAAAATCAAAATTGCTTCGGGATTCGGGCCATTGATCACACACGGTTCAGGGATGGACCTATACAAATCCAACCACAACAATGTGTATTGGCTGACTATCCCGCCAATGAAGAACCTAGCCTTAGGTGTAATCAACACATTGGAGTGGATACCGAGATTCAAGGTTAGTCCCTACCTCTTCACTGTCCCAATTAAGGAAGCAGGCGAAGACTGCCATGCCCCAACATACCTACCTGCGGAGGTGGATGGTGATGTCAAACTCAGTTCCAATCTGGTGATTCTACCTGGTCAAGATCTCCAATATGTTTTGGCAACCTACGATACTTCCAGGGTTGAACATGCTGTGGTTTATTACGTTTACAGCCCAAGCCGCTCATTTTCTTACTTTTATCCTTTTAGGTTGCCTATAAAGGGGGTCCCCATCGAATTACAAGTGGAATGCTTCACATGGGACCAAAAACTCTGGTGCCGTCACTTCTGTGTGCTTGCGGACTCAGAATCTGGTGGACATATCACTCACTCTGGGATGGTGGGCATGGGAGTCAGCTGCACAGTCACCCGGGAAGATGGAACCAATCGCAGATAGGGCTGCTAGTGAACCAATCACATGATGTCACCCAGACATCAGGCATACCCACTAGTGTGAAATAGACATCAGAATTAAGAAAAACGTAGGGTCCAAGTGGTTCCCCGTTATGGACTCGCTATCTGTCAACCAGATCTTATACCCTGAAGTTCACCTAGATAGCCCGATAGTTACCAATAAGATAGTAGCCATCCTGGAGTATGCTCGAGTCCCTCACGCTTACAGCCTGGAGGACCCTACACTGTGTCAGAACATCAAGCACCGCCTAAAAAACGGATTTTCCAACCAAATGATTATAAACAATGTGGAAGTTGGGAATGTCATCAAGTCCAAGCTTAGGAGTTATCCGGCCCACTCTCATATTCCATATCCAAATTGTAATCAGGATTTATTTAACATAGAAGACAAAGAGTCAACGAGGAAGATCCGTGAACTCCTCAAAAAGGGGAATTCGCTGTACTCCAAAGTCAGTGATAAGGTTTTCCAATGCTTAAGGGACACTAACTCACGGCTTGGCCTAGGCTCCGAATTGAGGGAGGACATCAAGGAGAAAGTTATTAACTTGGGAGTTTACATGCACAGCTCCCAGTGGTTTGAGCCCTTTCTGTTTTGGTTTACAGTCAAGACTGAGATGAGGTCAGTGATTAAATCACAAACCCATACTTGCCATAGGAGGAGACACACACCTGTATTCTTCACTGGTAGTTCAGTTGAGTTGCTAATCTCTCGTGACCTTGTTGCTATAATCAGTAAAGAGTCTCAACATGTATATTACCTGACATTTGAACTGGTTTTGATGTATTGTGATGTCATAGAGGGGAGGTTAATGACAGAGACCGCTATGACTATTGATGCTAGGTATACAGAGCTTCTAGGAAGAGTCAGATACATGTGGAAACTGATAGATGGTTTCTTCCCTGCACTCGGGAATCCAACTTATCAAATTGTAGCCATGCTGGAGCCTCTTTCACTTGCTTACCTGCAGCTGAGGGATATAACAGTAGAACTCAGAGGTGCTTTCCTTAACCACTGCTTTACTGAAATACATGATGTTCTTGACCAAAACGGGTTTTCTGATGAAGGTACTTATCATGAGTTAACTGAAGCTCTAGATTACATTTTCATAACTGATGACATACATCTGACAGGGGAGATTTTCTCATTTTTCAGAAGTTTCGGCCACCCCAGACTTGAAGCAGTAACGGCTGCTGAAAATGTTAGGAAATACATGAATCAGCCTAAAGTCATTGTGTATGAGACTCTGATGAAAGGTCATGCCATATTTTGTGGAATCATAATCAACGGCTATCGTGACAGGCACGGAGGCAGTTGGCCACCGCTGACCCTCCCCCTGCATGCTGCAGACACAATCCGGAATGCTCAAGCTTCAGGTGAAGGGTTAACACATGAGCAGTGCGTTGATAACTGGAAATCTTTTGCTGGAGTGAAATTTGGCTGCTTTATGCCTCTTAGCCTGGATAGTGATCTGACAATGTACCTAAAGGACAAGGCACTTGCTGCTCTCCAAAGGGAATGGGATTCAGTTTACCCGAAAGAGTTCCTGCGTTACGACCCTCCCAAGGGAACCGGGTCACGGAGGCTTGTAGATGTTTTCCTTAATGATTCGAGCTTTGACCCATATGATGTGATAATGTATGTTGTAAGTGGAGCTTACCTCCATGACCCTGAGTTCAACCTGTCTTACAGCCTGAAAGAAAAGGAGATCAAGGAAACAGGTAGACTTTTTGCTAAAATGACTTACAAAATGAGGGCATGCCAAGTGATTGCTGAAAATCTAATCTCAAACGGGATTGGCAAATATTTTAAGGACAATGGGATGGCCAAGGATGAGCACGATTTGACTAAGGCACTCCACACTCTAGCTGTCTCAGGAGTCCCCAAAGATCTCAAAGAAAGTCACAGGGGGGGGCCAGTCTTAAAAACCTACTCCCGAAGCCCAGTCCACACAAGTACCAGGAACGTGAGAGCAGCAAAAGGGTTTATAGGGTTCCCTCAAGTAATTCGGCAGGACCAAGACACTGATCATCCGGAGAATATGGAAGCTTACGAGACAGTCAGTGCATTTATCACGACTGATCTCAAGAAGTACTGCCTTAATTGGAGATATGAGACCATCAGCTTGTTTGCACAGAGGCTAAATGAGATTTACGGATTGCCCTCATTTTTCCAGTGGCTGCATAAGAGGCTTGAGACCTCTGTCCTGTATGTAAGTGACCCTCATTGCCCCCCCGACCTTGACGCCCATATCCCGTTATATAAAGTCCCCAATGATCAAATCTTCATTAAGTACCCTATGGGAGGTATAGAAGGGTATTGTCAGAAGCTGTGGACCATCAGCACCATTCCCTATCTATACCTGGCTGCTTATGAGAGCGGAGTAAGGATTGCTTCGTTAGTGCAAGGGGACAATCAGACCATAGCCGTAACAAAAAGGGTACCCAGCACATGGCCCTACAACCTTAAGAAACGGGAAGCTGCTAGAGTAACTAGAGATTACTTTGTAATTCTTAGGCAAAGGCTACATGATATTGGCCATCACCTCAAGGCAAATGAGACAATTGTTTCATCACATTTTTTTGTCTATTCAAAAGGAATATATTATGATGGGCTACTTGTGTCCCAATCACTCAAGAGCATCGCAAGATGTGTATTCTGGTCAGAGACTATAGTTGATGAAACAAGGGCAGCATGCAGTAATATTGCTACAACAATGGCTAAAAGCATCGAGAGAGGTTATGACCGTTACCTTGCATATTCCCTGAACGTCCTAAAAGTGATACAGCAAATTCTGATCTCTCTTGGCTTCACAATCAATTCAACCATGACCCGGGATGTAGTCATACCCCTCCTCACAAACAACGACCTCTTAATAAGGATGGCACTGTTGCCCGCTCCTATTGGGGGGATGAATTATCTGAATATGAGCAGGCTGTTTGTCAGAAACATCGGTGATCCAGTAACATCATCAATTGCTGATCTCAAGAGAATGATTCTCGCCTCACTAATGCCTGAAGAGACCCTCCATCAGGTAATGACACAACAACCGGGGGACTCTTCATTCCTAGACTGGGCTAGCGACCCTTACTCAGCAAATCTTGTATGTGTCCAGAGCATCACTAGACTCCTCAAGAACATAACTGCAAGGTTTGTCCTGATCCATAGTCCAAACCCAATGTTAAAAGGATTATTCCATGATGACAGTAAAGAAGAGGACGAGGGACTGGCGGCATTCCTCATGGACAGGCATATTATAGTACCTAGGGCAGCTCATGAAATCCTGGATCATAGTGTCACAGGGGCAAGAGAGTCTATTGCAGGCATGCTGGATACCACAAAAGGCTTGATTCGAGCCAGCATGAGGAAGGGGGGTTTAACCTCTCGAGTGATAACCAGATTGTCCAATTATGACTATGAACAATTCAGAGCAGGGATGGTGCTATTGACAGGAAGAAAGAGAAATGTCCTCATTGACAAAGAGTCATGTTCAGTGCAGCTGGCGAGAGCTCTAAGAAGCCATATGTGGGCGAGGCTAGCTCGAGGACGGCCTATTTACGGCCTTGAGGTCCCTGATGTACTAGAATCTATGCGAGGCCACCTTATTCGGCGTCATGAGACATGTGTCATCTGCGAGTGTGGATCAGTCAACTACGGATGGTTTTTTGTCCCCTCGGGTTGCCAACTGGATGATATTGACAAGGAAACATCATCCTTGAGAGTCCCATATATTGGTTCTACCACTGATGAGAGAACAGACATGAAGCTTGCCTTCGTAAGAGCCCCAAGTCGATCCTTGCGATCTGCTGTTAGAATAGCAACAGTGTACTCATGGGCTTACGGTGATGATGATAGCTCTTGGAACGAAGCCTGGTTGTTGGCTAGGCAAAGGGCCAATGTGAGCCTGGAGGAGCTAAGGGTGATCACTCCCATCTCAACTTCGACTAATTTAGCGCATAGGTTGAGGGATCGTAGCACTCAAGTGAAATACTCAGGTACATCCCTTGTCCGAGTGGCGAGGTATACCACAATCTCCAACGACAATCTCTCATTTGTCATATCAGATAAGAAGGTTGATACTAACTTTATATACCAACAAGGAATGCTTCTAGGGTTGGGTGTTTTAGAAACATTGTTTCGACTCGAGAAAGATACCGGATCATCTAACACGGTATTACATCTTCACGTCGAAACAGATTGTTGCGTGATCCCGATGATAGATCATCCCAGGATACCCAGCTCCCGCAAGCTAGAGCTGAGGGCAGAGCTATGTACCAACCCATTGATATATGATAATGCACCTTTAATTGACAGAGATGCAACAAGGCTATACACCCAGAGCCATAGGAGGCACCTTGTGGAATTTGTTACATGGTCCACACCCCAACTATATCACATTTTAGCTAAGTCCACAGCACTATCTATGATTGACCTGGTAACAAAATTTGAGAAGGACCATATGAATGAAATTTCAGCTCTCATAGGGGATGACGATATCAATAGTTTCATAACTGAGTTTCTGCTCATAGAGCCAAGATTATTCACTATCTACTTGGGCCAGTGTGCGGCCATCAATTGGGCATTTGATGTACATTATCATAGACCATCAGGGAAATATCAGATGGGTGAGCTGTTGTCATCGTTCCTTTCTAGAATGAGCAAAGGAGTGTTTAAGGTGCTTGTCAATGCTCTAAGCCACCCAAAGATCTACAAGAAATTCTGGCATTGTGGTATTATAGAGCCTATCCATGGTCCTTCACTTGATGCTCAAAACTTGCACACAACTGTGTGCAACATGGTTTACACATGCTATATGACCTACCTCGACCTGTTGTTGAATGAAGAGTTAGAAGAGTTCACATTTCTCTTGTGTGAAAGCGACGAGGATGTAGTACCGGACAGATTCGACAACATCCAGGCAAAACACTTATGTGTTCTGGCAGATTTGTACTGTCAACCAGGGACCTGCCCACCAATTCGAGGTCTAAGACCGGTAGAGAAATGTGCAGTTCTAACCGACCATATCAAGGCAGAGGCTATGTTATCTCCAGCAGGATCTTCGTGGAACATAAATCCAATTATTGTAGACCATTACTCATGCTCCCTGACTTATCTCCGGCGAGGATCGATCAAACAGATAAGATTGAGAGTTGATCCAGGATTCATTTTCGACGCCCTCGCTGAGGTAAATGTCAGTCAGCCAAAGATCGGCAGCAACAACATCTCAAATATGAGCATCAAGGCTTTCAGACCCCCACACGATGATGTTGCAAAATTGCTCAAAGATATCAACACAAGCAAGCACAATCTTCCCATTTCAGGGGGCAATCTCGCCAATTATGAAATCCATGCTTTCCGCAGAATCGGGTTGAACTCATCTGCTTGCTACAAAGCTGTTGAGATATCAACATTAATTAGGAGATGCCTTGAGCCAGGGGAGGACGGCTTGTTCTTGGGTGAGGGATCGGGTTCTATGTTGATCACTTATAAGGAGATACTTAAACTAAACAAGTGCTTCTATAATAGTGGGGTTTCCGCCAATTCTAGATCTGGTCAAAGGGAATTAGCACCCTATCCCTCCGAAGTTGGCCTTGTCGAACACAGAATGGGAGTAGGTAATATTGTCAAAGTGCTCTTTAACGGGAGGCCCGAAGTCACGTGGGTAGGCAGTGTAGATTGCTTCAATTTCATAGTTAGTAATATCCCTACCTCTAGTGTGGGGTTTATCCATTCAGATATAGAGACCTTGCCTGACAAAGATACTATAGAGAAGCTAGAGGAATTGGCAGCCATCTTATCGATGGCTCTGCTCCTGGGCAAAATAGGATCAATACTGGTGATTAAGCTTATGCCTTTCAGCGGGGATTTTGTTCAGGGATTTATAAGTTATGTAGGGTCTCATTATAGAGAAGTGAACCTTGTATACCCTAGATACAGCAACTTCATATCTACTGAATCTTATTTGGTTATGACAGATCTCAAGGCTAACCGGCTAATGAATCCTGAAAAGATTAAGCAGCAGATAATTGAATCATCTGTGAGGACTTCACCTGGACTTATAGGTCACATCCTATCCATTAAGCAACTAAGCTGCATACAAGCAATTGTGGGAGACGCAGTTAGTAGAGGTGATATCAATCCTACTCTGAAAAAACTTACACCTATAGAGCAGGTGCTGATCAATTGCGGGTTGGCAATTAACGGACCTAAGCTGTGCAAAGAATTGATCCACCATGATGTTGCCTCAGGGCAAGATGGATTGCTTAATTCTATACTCATCCTCTACAGGGAGTTGGCAAGATTCAAAGACAACCAAAGAAGTCAACAAGGGATGTTCCACGCCTACCCCGTATTGGTAAGTAGCAGGCAACGAGAACTTATATCTAGGATCACCCGCAAATTTTGGGGGCACATTCTTCTTTACTCCGGGAACAAAAAGTTGATAAATAAGTTTATCCAGAATCTCAAGTCCGGCTATCTGATACTAGACTTACACCAGAATATCTTCGTTAAGAATCTATCCAAGTCAGAGAAACAGATTATTATGACGGGGGGTTTGAAACGTGAGTGGGTTTTTAAGGTAACAGTCAAGGAGACCAAAGAATGGTATAAGTTAGTCGGATACAGTGCCCTGATTAAGGACTAATTGGTTGAACTCCGGAACCCTAATCCTGCCCTAGGTGGTTAGGCATTATTTGCAATATATTAAAGAAAACTTTGAAAATACGAAGTTTCTATTCCCAGCTTTGTCTGGTggccggcatAgtcccagcctcctcgctggcgctggctgggcaacattccgaggggaccgtccccAcggtaatggcgaatgggacgcggccgatccggctgctaacaaagcccgaaaggaagctgagttggctgctgGcGcTGgctgGgcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggatgcGGCCGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTG。
在仍另一个实施方式中，核酸包括SEQ ID NO：5的序列，如下所示：
GGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGCGCGCCGTTAATACGACTCACTATAGGGAGACCCAAGCTGGCTAGCTTTGTTTGGTCTGATGAGTCCCGTGAGGACGAAACCCGGCGTACCGGGTCACCAAACAAAGTTGGGTAAGGATAGTTCAATCAATGATCATCTTCTAGTGCACTTAGGATTCAAGATCCTATTATCAGGGACAAGAGCAGGATTAGGGATATCCGAGATGGCCACACTTTTAAGGAGCTTAGCATTGTTCAAAAGAAACAAGGACAAACCACCCATTACATCAGGATCCGGTGGAGCCATCAGAGGAATCAAACACATTATTATAGTACCAATCCCTGGAGATTCCTCAATTACCACTCGATCCAGACTTCTGGACCGGTTGGTGAGGTTAATTGGAAACCCGGATGTGAGCGGGCCCAAACTAACAGGGGCACTAATAGGTATATTATCCTTATTTGTGGAGTCTCCAGGTCAATTGATTCAGAGGATCACCGATGACCCTGACGTTAGCATAAGGCTGTTAGAGGTTGTCCAGAGTGACCAGTCACAATCTGGCCTTACCTTCGCATCAAGAGGTACCAACATGGAGGATGAGGCGGACCAATACTTTTCACATGATGATCCAATTAGTAGTGATCAATCCAGGTTCGGATGGTTCGGGAACAAGGAAATCTCAGATATTGAAGTGCAAGACCCTGAGGGATTCAACATGATTCTGGGTACCATCCTAGCCCAAATTTGGGTCTTGCTCGCAAAGGCGGTTACGGCCCCAGACACGGCAGCTGATTCGGAGCTAAGAAGGTGGATAAAGTACACCCAACAAAGAAGGGTAGTTGGTGAATTTAGATTGGAGAGAAAATGGTTGGATGTGGTGAGGAACAGGATTGCCGAGGACCTCTCCTTACGCCGATTCATGGTCGCTCTAATCCTGGATATCAAGAGAACACCCGGAAACAAACCCAGGATTGCTGAAATGATATGTGACATTGATACATATATCGTAGAGGCAGGATTAGCCAGTTTTATCCTGACTATTAAGTTTGGGATAGAAACTATGTATCCTGCTCTTGGACTGCATGAATTTGCTGGTGAGTTATCCACACTTGAGTCCTTGATGAACCTTTACCAGCAAATGGGGGAAACTGCACCCTACATGGTAATCCTGGAGAACTCAATTCAGAACAAGTTCAGTGCAGGATCATACCCTCTGCTCTGGAGCTATGCCATGGGAGTAGGAGTGGAACTTGAAAACTCCATGGGAGGTTTGAACTTTGGCCGATCTTACTTTGATCCAGCATATTTTAGATTAGGGCAAGAGATGGTAAGGAGGTCAGCTGGAAAGGTCAGTTCCACATTGGCATCTGAACTCGGTATCACTGCCGAGGATGCAAGGCTTGTTTCAGAGATTGCAATGCATACTACTGAGGACAAGATCAGTAGAGCGGTTGGACCCAGACAAGCCCAAGTATCATTTCTACACGGTGATCAAAGTGAGAATGAGCTACCGAGATTGGGGGGCAAGGAAGATAGGAGGGTCAAACAGAGTCGAGGAGAAGCCAGGGAGAGCTACAGAGAAACCGGGCCCAGCAGAGCAAGTGATGCGAGAGCTGCCCATCTTCCAACCGGCACACCCCTAGACATTGACACTGCAACGGAGTCCAGCCAAGATCCGCAGGACAGTCGAAGGTCAGCTGACGCCCTGCTTAGGCTGCAAGCCATGGCAGGAATCTCGGAAGAACAAGGCTCAGACACGGACACCCCTATAGTGTACAATGACAGAAATCTTCTAGACTAGGTGCGAGAGGCCGAGGGCCAGAACAACATCCGCCTACCATCCATCATTGTTATAAAAAACTTAGGAACCAGGTCCACACAGCCGCCAGCCCATCAACCATCCACTCCCACGATTGGAGCCAATGGCAGAAGAGCAGGCACGCCATGTCAAAAACGGACTGGAATGCATCCGGGCTCTCAAGGCCGAGCCCATCGGCTCACTGGCCATCGAGGAAGCTATGGCAGCATGGTCAGAAATATCAGACAACCCAGGACAGGAGCGAGCCACCTGCAGGGAAGAGAAGGCAGGCAGTTCGGGTCTCAGCAAACCATGCCTCTCAGCAATTGGATCAACTGAAGGCGGTGCACCTCGCATCCGCGGTCAGGGACCTGGAGAGAGCGATGACGACGCTGAAACTTTGGGAATCCCCCCAAGAAATCTCCAGGCATCAAGCACTGGGTTACAGTGTTATTACGTTTATGATCACAGCGGTGAAGCGGTTAAGGGAATCCAAGATGCTGACTCTATCATGGTTCAATCAGGCCTTGATGGTGATAGCACCCTCTCAGGAGGAGACAATGAATCTGAAAACAGCGATGTGGATATTGGCGAACCTGATACCGAGGGATATGCTATCACTGACCGGGGATCTGCTCCCATCTCTATGGGGTTCAGGGCTTCTGATGTTGAAACTGCAGAAGGAGGGGAGATCCACGAGCTCCTGAGACTCCAATCCAGAGGCAACAACTTTCCGAAGCTTGGGAAAACTCTCAATGTTCCTCCGCCCCCGGACCCCGGTAGGGCCAGCACTTCCGGGACACCCATTAAAAAGGGCACAGACGCGAGATTAGCCTCATTTGGAACGGAGATCGCGTCTTTATTGACAGGTGGTGCAACCCAATGTGCTCGAAAGTCACCCTCGGAACCATCAGGGCCAGGTGCACCTGCGGGGAATGTCCCCGAGTGTGTGAGCAATGCCGCACTGATACAGGAGTGGACACCCGAATCTGGTACCACAATCTCCCCGAGATCCCAGAATAATGAAGAAGGGGGAGACTATTATGATGATGAGCTGTTCTCTGATGTCCAAGATATTAAAACAGCCTTGGCCAAAATACACGAGGATAATCAGAAGATAATCTCCAAGCTAGAATCACTGCTGTTATTGAAGGGAGAAGTTGAGTCAATTAAGAAGCAGATCAACAGGCAAAATATCAGCATATCCACCCTGGAAGGACACCTCTCAAGCATCATGATCGCCATTCCTGGACTTGGGAAGGATCCCAACGACCCCACTGCAGATGTCGAAATCAATCCCGACTTGAAACCCATCATAGGCAGAGATTCAGGCCGAGCACTGGCCGAAGTTCTCAAGAAACCCGTTGCCAGCCGACAACTCCAAGGAATGACAAATGGACGGACCAGTTCCAGAGGACAGCTGCTGAAGGAATTTCAGCTAAAGCCGATCGGGAAAAAGATGAGCTCAGCCGTCGGGTTTGTTCCTGACACCGGCCCTGCATCACGCAGTGTAATCCGCTCCATTATAAAATCCAGCCGGCTAGAGGAGGATCGGAAGCGTTACCTGATGACTCTCCTTGATGATATCAAAGGAGCCAATGATCTTGCCAAGTTCCACCAGATGCTGATGAAGATAATAATGAAGTAGCTACAGCTCAACTTACCTGCCAACCCCATGCCAGTCGACCCACCTAGTACAACCTAAATCCATTATAAAAAACTTAGGAGCAAAGTGATTGCCTCCCAAGGTCCACAATGACAGAGACCTACGACTTCGACAAGTCGGCATGGGACATCAAAGGGTCGATCGCTCCGATACAACCCACCACCTACAGTGATGGCAGGCTGGTGCCCCAGGTCAGAGTCATAGATCCTGGTCTAGGCGACAGGAAGGATGAATGCTTTATGTACATGTTTCTGCTGGGGGTTGTTGAGGACAGCGATTCCCTAGGGCCTCCAATCGGGCGAGCATTTGGGTTCCTGCCCTTAGGTGTTGGCAGATCCACAGCAAAGCCCGAAAAACTCCTCAAAGAGGCCACTGAGCTTGACATAGTTGTTAGACGTACAGCAGGGCTCAATGAAAAACTGGTGTTCTACAACAACACCCCACTAACTCTCCTCACACCTTGGAGAAAGGTCCTAACAACAGGGAGTGTCTTCAACGCAAACCAAGTGTGCAATGCGGTTAATCTGATACCGCTCGATACCCCGCAGAGGTTCCGTGTTGTTTATATGAGCATCACCCGTCTTTCGGATAACGGGTATTACACCGTTCCTAGAAGAATGCTGGAATTCAGATCGGTCAATGCAGTGGCCTTCAACCTGCTGGTGACCCTTAGGATTGACAAGGCGATAGGCCCTGGGAAGATCATCGACAATACAGAGCAACTTCCTGAGGCAACATTTATGGTCCACATCGGGAACTTCAGGAGAAAGAAGAGTGAAGTCTACTCTGCCGATTATTGCAAAATGAAAATCGAAAAGATGGGCCTGGTTTTTGCACTTGGTGGGATAGGGGGCACCAGTCTTCACATTAGAAGCACAGGCAAAATGAGCAAGACTCTCCATGCACAACTCGGGTTCAAGAAGACCTTATGTTACCCGCTGATGGATATCAATGAAGACCTTAATCGATTACTCTGGAGGAGCAGATGCAAGATAGTAAGAATCCAGGCAGTTTTGCAGCCATCAGTTCCTCAAGAATTCCGCATTTACGACGACGTGATCATAAATGATGACCAAGGACTATTCAAAGTTCTGTAGACCGTAGTGCCCAGCAATGCCCGAAAACGACCCCCCTCACAATGACAGCCAGAAGGCCCGGACAAAAAAGCCCCCTCCGAAAGACTCCACGGACCAAGCGAGAGGCCAGCCAGCAGCCGACGGCAAGCGCGAACACCAGGCGGCCCCAGCACAGAACAGCCCTGACACAAGGCCACCACCAGCCACCCCAATCTGCATCCTCCTCGTGGGACCCCCGAGGACCAACCCCCAAGGCTGCCCCCGATCCAAACCACCAACCGCATCCCCACCACCCCCGGGAAAGAAACCCCCAGCAATTGGAAGGCCCCTCCCCCTCTTCCTCAACACAAGAACTCCACAACCGAACCGCACAAGCGACCGAGGTGACCCAACCGCAGGCATCCGACTCCCTAGACAGATCCTCTCTCCCCGGCAAACTAAACAAAACTTAGGGCCAAGGAACATACACACCCAACAGAACCCAGACCCCGGCCCACGGCGCCGCGCCCCCAACCCCCGACAACCAGAGGGAGCCCCCAACCAATCCCGCCGGCTCCCCCGGTGCCCACAGGCAGGGACACCAACCCCCGAACAGACCCAGCACCCAACCATCGACAATCCAAGACGGGGGGGCCCCCCCAAAAAAAGGCCCCCAGGGGCCGACAGCCAGCACCGCGAGGAAGCCCACCCACCCCACACACGACCACGGCAACCAAACCAGAACCCAGACCACCCTGGGCCACCAGCTCCCAGACTCGGCCATCACCCCGCAGAAAGGAAAGGCCACAACCCGCGCACCCCAGCCCCGATCCGGCGGGGAGCCACCCAACCCGAACCAGCACCCAAGAGCGATCCCCGAAGGACCCCCGAACCGCAAAGGACATCAGTATCCCACAGCCTCTCCAAGTCCCCCGGTCTCCTCCTCTTCTCGAAGGGACCAAAAGATCAATCCACCACACCCGACGACACTCAACTCCCCACCCCTAAAGGAGACACCGGGAATCCCAGAATCAAGACTCATCCAATGTCCATCATGGGTCTCAAGGTGAACGTCTCTGCCATATTCATGGCAGTACTGTTAACTCTCCAAACACCCACCGGTCAAATCCATTGGGGCAATCTCTCTAAGATAGGGGTGGTAGGAATAGGAAGTGCAAGCTACAAAGTTATGACTCGTTCCAGCCATCAATCATTAGTCATAAAATTAATGCCCAATATAACTCTCCTCAATAACTGCACGAGGGTAGAGATTGCAGAATACAGGAGACTACTGAGAACAGTTTTGGAACCAATTAGAGATGCACTTAATGCAATGACCCAGAATATAAGACCGGTTCAGAGTGTAGCTTCAAGTAGGAGACACAAGAGATTTGCGGGAGTAGTCCTGGCAGGTGCGGCCCTAGGCGTTGCCACAGCTGCTCAGATAACAGCCGGCATTGCACTTCACCAGTCCATGCTGAACTCTCAAGCCATCGACAATCTGAGAGCGAGCCTGGAAACTACTAATCAGGCAATTGAGACAATCAGACAAGCAGGGCAGGAGATGATATTGGCTGTTCAGGGTGTCCAAGACTACATCAATAATGAGCTGATACCGTCTATGAACCAACTATCTTGTGATTTAATCGGCCAGAAGCTCGGGCTCAAATTGCTCAGATACTATACAGAAATCCTGTCATTATTTGGCCCCAGTTTACGGGACCCCATATCTGCGGAGATATCTATCCAGGCTTTGAGCTATGCGCTTGGAGGAGACATCAATAAGGTGTTAGAAAAGCTCGGATACAGTGGAGGTGATTTACTGGGCATCTTAGAGAGCGGAGGAATAAAGGCCCGGATAACTCACGTCGACACAGAGTCCTACTTCATTGTCCTCAGTATAGCCTATCCGACGCTGTCCGAGATTAAGGGGGTGATTGTCCACCGGCTAGAGGGGGTCTCGTACAACATAGGCTCTCAAGAGTGGTATACCACTGTGCCCAAGTATGTTGCAACCCAAGGGTACCTTATCTCGAATTTTGATGAGTCATCGTGTACTTTCATGCCAGAGGGGACTGTGTGCAGCCAAAATGCCTTGTACCCGATGAGTCCTCTGCTCCAAGAATGCCTCCGGGGGTACACCAAGTCCTGTGCTCGTACACTCGTATCCGGGTCTTTTGGGAACCGGTTCATTTTATCACAAGGGAACCTAATAGCCAATTGTGCATCAATCCTTTGCAAGTGTTACACAACAGGAACGATCATTAATCAAGACCCTGACAAGATCCTAACATACATTGCTGCCGATCACTGCCCGGTAGTCGAGGTGAACGGCGTGACCATCCAAGTCGGGAGCAGGAGGTATCCAGACGCTGTGTACTTGCACAGAATTGACCTCGGTCCTCCCATATCATTGGAGAGGTTGGACGTAGGGACAAATCTGGGGAATGCAATTGCTAAGTTGGAGGATGCCAAGGAATTGTTGGAGTCATCGGACCAGATATTGAGGAGTATGAAAGGTTTATCGAGCACTAGCATAGTCTACATCCTGATTGCAGTGTGTCTTGGAGGGTTGATAGGGATCCCCGCTTTAATATGTTGCTGCAGGGGGCGTTGTAACAAAAAGGGAGAACAAGTTGGTATGTCAAGACCAGGCCTAAAGCCTGATCTTACGGGAACATCAAAATCCTATGTAAGGTCGCTCTGATCCTCTACAACTCTTGAAACACAAATGTCCCACAAGTCTCCTCTTCGTCATCAAGCAACCACCGCACCCAGCATCAAGCCCACCTGAAATTATCTCCGGCTTCCCTCTGGCCGAACAATATCGGTAGTTAATTAAAACTTAGGGTGCAAGATCATCGATAATGTCACCACAACGAGACCGGATAAATGCCTTCTACAAAGATAACCCCCATCCCAAGGGAAGTAGGATAGTCATTAACAGAGAACATCTTATGATTGATAGACCTTATGTTTTGCTGGCTGTTCTGTTTGTCATGTTTCTGAGCTTGATCGGGTTGCTAGCCATTGCAGGAATTCGACTTCATCGGGCAGCCATCTACACCGCAGAGATCCATAAAAGCCTCAGCACCAATCTAGATGTAACTAACTCAATCGAGCATCAGGTCAAGGACGTGCTGACACCACTCTTCAAAATCATCGGTGATGAAGTGGGCCTGAGGACACCTCAGAGATTCACTGACCTAGTGAAATTAATCTCTGACAAGATTAAATTCCTTAATCCGGATAGGGAGTACGACTTCAGAGATCTCACTTGGTGTATCAACCCGCCAGAGAGAATCAAATTGGATTATGATCAATACTGTGCAGATGTGGCTGCTGAAGAGCTCATGAATGCATTGGTGAACTCAACTCTACTGGAGACCAGAACAACCAATCAGTTCCTAGCTGTCTCAAAGGGAAACTGCTCAGGGCCCACTACAATCAGAGGTCAATTCTCAAACATGTCGCTGTCCCTGTTAGACTTGTATTTAGGTCGAGGTTACAATGTGTCATCTATAGTCACTATGACATCCCAGGGAATGTATGGGGGAACTTACCTAGTGGAAAAGCCTAATCTGAGCAGCAAAAGGTCAGAGTTGTCACAACTGAGCATGTACCGAGTGTTTGAAGTAGGTGTTATCAGAAATCCGGGTTTGGGGGCTCCGGTGTTCCATATGACAAACTATCTTGAGCAACCAGTCAGTAATGATCTCAGCAACTGTATGGTGGCTTTGGGGGAGCTCAAACTCGCAGCCCTTTGTCACGGGGAAGATTCTATCACAATTCCCTATCAGGGATCAGGGAAAGGTGTCAGCTTCCAGCTCGTCAAGCTAGGTGTCTGGAAATCCCCAACCGACATGCAATCCTGGGTCCCCTTATCAACGGATGATCCAGTGATAGACAGGCTTTACCTCTCATCTCACAGAGGTGTTATCGCTGACAACCAAGCAAAATGGGCTGTCCCGACAACACGAACAGATGACAAGTTGCGAATGGAGACATGCTTCCAACAGGCGTGTAAGGGTAAAATCCAAGCACTCTGCGAGAATCCCGAGTGGGCACCATTGAAGGATAACAGGATTCCTTCATACGGGGTCTTGTCTGTTGATCTGAGTCTGACAGTTGAGCTTAAAATCAAAATTGCTTCGGGATTCGGGCCATTGATCACACACGGTTCAGGGATGGACCTATACAAATCCAACCACAACAATGTGTATTGGCTGACTATCCCGCCAATGAAGAACCTAGCCTTAGGTGTAATCAACACATTGGAGTGGATACCGAGATTCAAGGTTAGTCCCTACCTCTTCACTGTCCCAATTAAGGAAGCAGGCGAAGACTGCCATGCCCCAACATACCTACCTGCGGAGGTGGATGGTGATGTCAAACTCAGTTCCAATCTGGTGATTCTACCTGGTCAAGATCTCCAATATGTTTTGGCAACCTACGATACTTCCAGGGTTGAACATGCTGTGGTTTATTACGTTTACAGCCCAAGCCGCTCATTTTCTTACTTTTATCCTTTTAGGTTGCCTATAAAGGGGGTCCCCATCGAATTACAAGTGGAATGCTTCACATGGGACCAAAAACTCTGGTGCCGTCACTTCTGTGTGCTTGCGGACTCAGAATCTGGTGGACATATCACTCACTCTGGGATGGTGGGCATGGGAGTCAGCTGCACAGTCACCCGGGAAGATGGAACCAATCGCAGATAGGGCTGCTAGTGAACCAATCACATGATGTCACCCAGACATCAGGCATACCCACCATCCATCATTGTTATAAAAAACTTAGGAACCAGGTCCACACAGAGTGATACGCGTACGCCACCATGTTCGTGTTTCTGGTGCTGCTGCCTCTGGTGAGCTCCCAGTGCGTGAACCTGACCACAAGGACCCAGCTGCCCCCTGCCTATACCAATTCCTTCACACGGGGCGTGTACTATCCCGACAAGGTGTTCCGGAGCAGCGTGCTGCACTCCACACAGGATCTGTTTCTGCCTTTCTTTTCTAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAATGGCACAAAGCGGTTCGACAATCCAGTGCTGCCCTTTAACGATGGCGTGTACTTCGCCTCCACCGAGAAGTCTAACATCATCAGAGGCTGGATCTTTGGCACCACACTGGACAGCAAGACACAGTCCCTGCTGATCGTGAACAATGCCACCAACGTGGTCATCAAGGTGTGCGAGTTCCAGTTTTGTAATGATCCATTCCTGGGCGTGTACTATCACAAGAACAATAAGTCTTGGATGGAGAGCGAGTTTCGCGTGTATTCCTCTGCCAACAATTGCACATTTGAGTACGTGTCCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAATTTCAAGAACCTGAGGGAGTTCGTGTTTAAGAATATCGATGGCTACTTCAAAATCTACTCCAAGCACACCCCAATCAACCTGGTGCGCGACCTGCCACAGGGCTTCTCTGCCCTGGAGCCACTGGTGGATCTGCCCATCGGCATCAACATCACCCGGTTTCAGACACTGCTGGCCCTGCACAGAAGCTACCTGACACCAGGCGACAGCTCCTCTGGATGGACCGCAGGAGCAGCAGCCTACTATGTGGGCTATCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAATGGCACCATCACAGACGCCGTGGATTGCGCCCTGGATCCCCTGTCTGAGACCAAGTGTACACTGAAGAGCTTTACCGTGGAGAAGGGCATCTATCAGACAAGCAATTTCAGGGTGCAGCCTACCGAGTCCATCGTGCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAAGATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGGAGGGCTTTAACTGTTATTTCCCTCTGCAGAGCTACGGCTTCCAGCCAACAAACGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACCTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGACTGACCGGAACAGGCGTGCTGACCGAGTCCAACAAGAAGTTCCTGCCTTTTCAGCAGTTCGGCAGGGACATCGCAGATACCACAGACGCCGTGCGCGACCCTCAGACCCTGGAGATCCTGGACATCACACCATGCTCCTTCGGCGGCGTGTCTGTGATCACACCAGGCACCAATACAAGCAACCAGGTGGCCGTGCTGTATCAGGACGTGAATTGTACCGAGGTGCCAGTGGCAATCCACGCAGATCAGCTGACCCCTACATGGCGGGTGTACTCTACCGGCAGCAACGTGTTCCAGACAAGAGCCGGATGCCTGATCGGAGCAGAGCACGTGAACAATAGCTATGAGTGCGACATCCCTATCGGCGCCGGCATCTGTGCCTCCTACCAGACCCAGACAAACTCCCCAAGGAGAGCACGGTCTGTGGCCAGCCAGTCCATCATCGCCTATACCATGAGCCTGGGCGCCGAGAATTCCGTGGCCTACTCCAACAATTCTATCGCCATCCCTACCAACTTCACAATCTCCGTGACCACAGAGATCCTGCCAGTGAGCATGACCAAGACATCCGTGGACTGCACAATGTATATCTGTGGCGATTCCACCGAGTGCTCTAACCTGCTGCTGCAGTACGGCTCTTTTTGTACCCAGCTGAATAGAGCCCTGACAGGCATCGCCGTGGAGCAGGACAAGAACACACAGGAGGTGTTCGCCCAGGTGAAGCAAATCTACAAGACCCCACCCATCAAGGACTTTGGCGGCTTCAACTTCAGCCAGATCCTGCCCGATCCTAGCAAGCCATCCAAGCGGTCTTTTATCGAGGACCTGCTGTTCAACAAGGTGACCCTGGCCGATGCCGGCTTCATCAAGCAGTATGGCGATTGCCTGGGCGACATCGCCGCCAGAGACCTGATCTGTGCCCAGAAGTTTAATGGCCTGACCGTGCTGCCTCCACTGCTGACAGATGAGATGATCGCCCAGTACACATCTGCCCTGCTGGCCGGAACCATCACAAGCGGATGGACCTTCGGCGCAGGAGCCGCCCTGCAGATCCCCTTTGCCATGCAGATGGCCTATCGGTTCAACGGCATCGGCGTGACCCAGAATGTGCTGTACGAGAACCAGAAGCTGATCGCCAATCAGTTTAACTCCGCCATCGGCAAGATCCAGGACTCTCTGAGCTCCACAGCCAGCGCCCTGGGCAAGCTGCAGGATGTGGTGAATCAGAACGCCCAGGCCCTGAATACCCTGGTGAAGCAGCTGTCTAGCAACTTCGGCGCCATCTCCTCTGTGCTGAATGACATCCTGAGCCGGCTGGACAAGGTGGAGGCAGAGGTGCAGATCGACCGGCTGATCACAGGCAGACTGCAGTCCCTGCAGACCTACGTGACACAGCAGCTGATCAGGGCAGCAGAGATCAGGGCCTCTGCCAATCTGGCCGCCACCAAGATGAGCGAGTGCGTGCTGGGCCAGTCCAAGAGAGTGGACTTTTGTGGCAAGGGCTATCACCTGATGAGCTTCCCACAGTCCGCCCCTCACGGAGTGGTGTTTCTGCACGTGACCTACGTGCCAGCCCAGGAGAAGAACTTCACCACAGCACCAGCAATCTGCCACGATGGCAAGGCACACTTTCCTAGGGAGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTTGTGACACAGCGCAATTTCTACGAGCCACAGATCATCACCACAGACAATACATTCGTGTCCGGCAACTGTGACGTGGTCATCGGCATCGTGAACAATACCGTGTATGATCCTCTGCAGCCAGAGCTGGACTCTTTTAAGGAGGAGCTGGATAAGTACTTCAAGAATCACACCAGCCCCGACGTGGATCTGGGCGACATCTCTGGCATCAATGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAGGTGGCCAAGAATCTGAACGAGTCCCTGATCGATCTGCAGGAGCTGGGCAAGTATGAGCAGTACATCAAGTGGCCCTGGTATATCTGGCTGGGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTGTATGACAAGCTGCTGTTCCTGCCTGAAGGGCTGCTGTTCTTGTGGCAGCTGCTGTAAGTTTGATGAGGACGATAGCGAGCCTGTGCTGAAGGGCGTGAAGCTGCACTACACCTGAGCTAGCGATCGCACTAGTGTGAAATAGACATCAGAATTAAGAAAAACGTAGGGTCCAAGTGGTTCCCCGTTATGGACTCGCTATCTGTCAACCAGATCTTATACCCTGAAGTTCACCTAGATAGCCCGATAGTTACCAATAAGATAGTAGCCATCCTGGAGTATGCTCGAGTCCCTCACGCTTACAGCCTGGAGGACCCTACACTGTGTCAGAACATCAAGCACCGCCTAAAAAACGGATTTTCCAACCAAATGATTATAAACAATGTGGAAGTTGGGAATGTCATCAAGTCCAAGCTTAGGAGTTATCCGGCCCACTCTCATATTCCATATCCAAATTGTAATCAGGATTTATTTAACATAGAAGACAAAGAGTCAACGAGGAAGATCCGTGAACTCCTCAAAAAGGGGAATTCGCTGTACTCCAAAGTCAGTGATAAGGTTTTCCAATGCTTAAGGGACACTAACTCACGGCTTGGCCTAGGCTCCGAATTGAGGGAGGACATCAAGGAGAAAGTTATTAACTTGGGAGTTTACATGCACAGCTCCCAGTGGTTTGAGCCCTTTCTGTTTTGGTTTACAGTCAAGACTGAGATGAGGTCAGTGATTAAATCACAAACCCATACTTGCCATAGGAGGAGACACACACCTGTATTCTTCACTGGTAGTTCAGTTGAGTTGCTAATCTCTCGTGACCTTGTTGCTATAATCAGTAAAGAGTCTCAACATGTATATTACCTGACATTTGAACTGGTTTTGATGTATTGTGATGTCATAGAGGGGAGGTTAATGACAGAGACCGCTATGACTATTGATGCTAGGTATACAGAGCTTCTAGGAAGAGTCAGATACATGTGGAAACTGATAGATGGTTTCTTCCCTGCACTCGGGAATCCAACTTATCAAATTGTAGCCATGCTGGAGCCTCTTTCACTTGCTTACCTGCAGCTGAGGGATATAACAGTAGAACTCAGAGGTGCTTTCCTTAACCACTGCTTTACTGAAATACATGATGTTCTTGACCAAAACGGGTTTTCTGATGAAGGTACTTATCATGAGTTAACTGAAGCTCTAGATTACATTTTCATAACTGATGACATACATCTGACAGGGGAGATTTTCTCATTTTTCAGAAGTTTCGGCCACCCCAGACTTGAAGCAGTAACGGCTGCTGAAAATGTTAGGAAATACATGAATCAGCCTAAAGTCATTGTGTATGAGACTCTGATGAAAGGTCATGCCATATTTTGTGGAATCATAATCAACGGCTATCGTGACAGGCACGGAGGCAGTTGGCCACCGCTGACCCTCCCCCTGCATGCTGCAGACACAATCCGGAATGCTCAAGCTTCAGGTGAAGGGTTAACACATGAGCAGTGCGTTGATAACTGGAAATCTTTTGCTGGAGTGAAATTTGGCTGCTTTATGCCTCTTAGCCTGGATAGTGATCTGACAATGTACCTAAAGGACAAGGCACTTGCTGCTCTCCAAAGGGAATGGGATTCAGTTTACCCGAAAGAGTTCCTGCGTTACGACCCTCCCAAGGGAACCGGGTCACGGAGGCTTGTAGATGTTTTCCTTAATGATTCGAGCTTTGACCCATATGATGTGATAATGTATGTTGTAAGTGGAGCTTACCTCCATGACCCTGAGTTCAACCTGTCTTACAGCCTGAAAGAAAAGGAGATCAAGGAAACAGGTAGACTTTTTGCTAAAATGACTTACAAAATGAGGGCATGCCAAGTGATTGCTGAAAATCTAATCTCAAACGGGATTGGCAAATATTTTAAGGACAATGGGATGGCCAAGGATGAGCACGATTTGACTAAGGCACTCCACACTCTAGCTGTCTCAGGAGTCCCCAAAGATCTCAAAGAAAGTCACAGGGGGGGGCCAGTCTTAAAAACCTACTCCCGAAGCCCAGTCCACACAAGTACCAGGAACGTGAGAGCAGCAAAAGGGTTTATAGGGTTCCCTCAAGTAATTCGGCAGGACCAAGACACTGATCATCCGGAGAATATGGAAGCTTACGAGACAGTCAGTGCATTTATCACGACTGATCTCAAGAAGTACTGCCTTAATTGGAGATATGAGACCATCAGCTTGTTTGCACAGAGGCTAAATGAGATTTACGGATTGCCCTCATTTTTCCAGTGGCTGCATAAGAGGCTTGAGACCTCTGTCCTGTATGTAAGTGACCCTCATTGCCCCCCCGACCTTGACGCCCATATCCCGTTATATAAAGTCCCCAATGATCAAATCTTCATTAAGTACCCTATGGGAGGTATAGAAGGGTATTGTCAGAAGCTGTGGACCATCAGCACCATTCCCTATCTATACCTGGCTGCTTATGAGAGCGGAGTAAGGATTGCTTCGTTAGTGCAAGGGGACAATCAGACCATAGCCGTAACAAAAAGGGTACCCAGCACATGGCCCTACAACCTTAAGAAACGGGAAGCTGCTAGAGTAACTAGAGATTACTTTGTAATTCTTAGGCAAAGGCTACATGATATTGGCCATCACCTCAAGGCAAATGAGACAATTGTTTCATCACATTTTTTTGTCTATTCAAAAGGAATATATTATGATGGGCTACTTGTGTCCCAATCACTCAAGAGCATCGCAAGATGTGTATTCTGGTCAGAGACTATAGTTGATGAAACAAGGGCAGCATGCAGTAATATTGCTACAACAATGGCTAAAAGCATCGAGAGAGGTTATGACCGTTACCTTGCATATTCCCTGAACGTCCTAAAAGTGATACAGCAAATTCTGATCTCTCTTGGCTTCACAATCAATTCAACCATGACCCGGGATGTAGTCATACCCCTCCTCACAAACAACGACCTCTTAATAAGGATGGCACTGTTGCCCGCTCCTATTGGGGGGATGAATTATCTGAATATGAGCAGGCTGTTTGTCAGAAACATCGGTGATCCAGTAACATCATCAATTGCTGATCTCAAGAGAATGATTCTCGCCTCACTAATGCCTGAAGAGACCCTCCATCAGGTAATGACACAACAACCGGGGGACTCTTCATTCCTAGACTGGGCTAGCGACCCTTACTCAGCAAATCTTGTATGTGTCCAGAGCATCACTAGACTCCTCAAGAACATAACTGCAAGGTTTGTCCTGATCCATAGTCCAAACCCAATGTTAAAAGGATTATTCCATGATGACAGTAAAGAAGAGGACGAGGGACTGGCGGCATTCCTCATGGACAGGCATATTATAGTACCTAGGGCAGCTCATGAAATCCTGGATCATAGTGTCACAGGGGCAAGAGAGTCTATTGCAGGCATGCTGGATACCACAAAAGGCTTGATTCGAGCCAGCATGAGGAAGGGGGGTTTAACCTCTCGAGTGATAACCAGATTGTCCAATTATGACTATGAACAATTCAGAGCAGGGATGGTGCTATTGACAGGAAGAAAGAGAAATGTCCTCATTGACAAAGAGTCATGTTCAGTGCAGCTGGCGAGAGCTCTAAGAAGCCATATGTGGGCGAGGCTAGCTCGAGGACGGCCTATTTACGGCCTTGAGGTCCCTGATGTACTAGAATCTATGCGAGGCCACCTTATTCGGCGTCATGAGACATGTGTCATCTGCGAGTGTGGATCAGTCAACTACGGATGGTTTTTTGTCCCCTCGGGTTGCCAACTGGATGATATTGACAAGGAAACATCATCCTTGAGAGTCCCATATATTGGTTCTACCACTGATGAGAGAACAGACATGAAGCTTGCCTTCGTAAGAGCCCCAAGTCGATCCTTGCGATCTGCTGTTAGAATAGCAACAGTGTACTCATGGGCTTACGGTGATGATGATAGCTCTTGGAACGAAGCCTGGTTGTTGGCTAGGCAAAGGGCCAATGTGAGCCTGGAGGAGCTAAGGGTGATCACTCCCATCTCAACTTCGACTAATTTAGCGCATAGGTTGAGGGATCGTAGCACTCAAGTGAAATACTCAGGTACATCCCTTGTCCGAGTGGCGAGGTATACCACAATCTCCAACGACAATCTCTCATTTGTCATATCAGATAAGAAGGTTGATACTAACTTTATATACCAACAAGGAATGCTTCTAGGGTTGGGTGTTTTAGAAACATTGTTTCGACTCGAGAAAGATACCGGATCATCTAACACGGTATTACATCTTCACGTCGAAACAGATTGTTGCGTGATCCCGATGATAGATCATCCCAGGATACCCAGCTCCCGCAAGCTAGAGCTGAGGGCAGAGCTATGTACCAACCCATTGATATATGATAATGCACCTTTAATTGACAGAGATGCAACAAGGCTATACACCCAGAGCCATAGGAGGCACCTTGTGGAATTTGTTACATGGTCCACACCCCAACTATATCACATTTTAGCTAAGTCCACAGCACTATCTATGATTGACCTGGTAACAAAATTTGAGAAGGACCATATGAATGAAATTTCAGCTCTCATAGGGGATGACGATATCAATAGTTTCATAACTGAGTTTCTGCTCATAGAGCCAAGATTATTCACTATCTACTTGGGCCAGTGTGCGGCCATCAATTGGGCATTTGATGTACATTATCATAGACCATCAGGGAAATATCAGATGGGTGAGCTGTTGTCATCGTTCCTTTCTAGAATGAGCAAAGGAGTGTTTAAGGTGCTTGTCAATGCTCTAAGCCACCCAAAGATCTACAAGAAATTCTGGCATTGTGGTATTATAGAGCCTATCCATGGTCCTTCACTTGATGCTCAAAACTTGCACACAACTGTGTGCAACATGGTTTACACATGCTATATGACCTACCTCGACCTGTTGTTGAATGAAGAGTTAGAAGAGTTCACATTTCTCTTGTGTGAAAGCGACGAGGATGTAGTACCGGACAGATTCGACAACATCCAGGCAAAACACTTATGTGTTCTGGCAGATTTGTACTGTCAACCAGGGACCTGCCCACCAATTCGAGGTCTAAGACCGGTAGAGAAATGTGCAGTTCTAACCGACCATATCAAGGCAGAGGCTATGTTATCTCCAGCAGGATCTTCGTGGAACATAAATCCAATTATTGTAGACCATTACTCATGCTCCCTGACTTATCTCCGGCGAGGATCGATCAAACAGATAAGATTGAGAGTTGATCCAGGATTCATTTTCGACGCCCTCGCTGAGGTAAATGTCAGTCAGCCAAAGATCGGCAGCAACAACATCTCAAATATGAGCATCAAGGCTTTCAGACCCCCACACGATGATGTTGCAAAATTGCTCAAAGATATCAACACAAGCAAGCACAATCTTCCCATTTCAGGGGGCAATCTCGCCAATTATGAAATCCATGCTTTCCGCAGAATCGGGTTGAACTCATCTGCTTGCTACAAAGCTGTTGAGATATCAACATTAATTAGGAGATGCCTTGAGCCAGGGGAGGACGGCTTGTTCTTGGGTGAGGGATCGGGTTCTATGTTGATCACTTATAAGGAGATACTTAAACTAAACAAGTGCTTCTATAATAGTGGGGTTTCCGCCAATTCTAGATCTGGTCAAAGGGAATTAGCACCCTATCCCTCCGAAGTTGGCCTTGTCGAACACAGAATGGGAGTAGGTAATATTGTCAAAGTGCTCTTTAACGGGAGGCCCGAAGTCACGTGGGTAGGCAGTGTAGATTGCTTCAATTTCATAGTTAGTAATATCCCTACCTCTAGTGTGGGGTTTATCCATTCAGATATAGAGACCTTGCCTGACAAAGATACTATAGAGAAGCTAGAGGAATTGGCAGCCATCTTATCGATGGCTCTGCTCCTGGGCAAAATAGGATCAATACTGGTGATTAAGCTTATGCCTTTCAGCGGGGATTTTGTTCAGGGATTTATAAGTTATGTAGGGTCTCATTATAGAGAAGTGAACCTTGTATACCCTAGATACAGCAACTTCATATCTACTGAATCTTATTTGGTTATGACAGATCTCAAGGCTAACCGGCTAATGAATCCTGAAAAGATTAAGCAGCAGATAATTGAATCATCTGTGAGGACTTCACCTGGACTTATAGGTCACATCCTATCCATTAAGCAACTAAGCTGCATACAAGCAATTGTGGGAGACGCAGTTAGTAGAGGTGATATCAATCCTACTCTGAAAAAACTTACACCTATAGAGCAGGTGCTGATCAATTGCGGGTTGGCAATTAACGGACCTAAGCTGTGCAAAGAATTGATCCACCATGATGTTGCCTCAGGGCAAGATGGATTGCTTAATTCTATACTCATCCTCTACAGGGAGTTGGCAAGATTCAAAGACAACCAAAGAAGTCAACAAGGGATGTTCCACGCCTACCCCGTATTGGTAAGTAGCAGGCAACGAGAACTTATATCTAGGATCACCCGCAAATTTTGGGGGCACATTCTTCTTTACTCCGGGAACAAAAAGTTGATAAATAAGTTTATCCAGAATCTCAAGTCCGGCTATCTGATACTAGACTTACACCAGAATATCTTCGTTAAGAATCTATCCAAGTCAGAGAAACAGATTATTATGACGGGGGGTTTGAAACGTGAGTGGGTTTTTAAGGTAACAGTCAAGGAGACCAAAGAATGGTATAAGTTAGTCGGATACAGTGCCCTGATTAAGGACTAATTGGTTGAACTCCGGAACCCTAATCCTGCCCTAGGTGGTTAGGCATTATTTGCAATATATTAAAGAAAACTTTGAAAATACGAAGTTTCTATTCCCAGCTTTGTCTGGTggccggcatAgtcccagcctcctcgctggcgctggctgggcaacattccgaggggaccgtccccAcggtaatggcgaatgggacgcggccgatccggctgctaacaaagcccgaaaggaagctgagttggctgctgGcGcTGgctgGgcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggatgcGGCCGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTG。
在一个实施方式中，核酸包括SEQ ID NO：6的序列，如下所示(RABV载体：CoravaxV1-China(RABVG-E31))：
ACGCTTAACAACCAGATCAAAGAAAAAACAGACATTGTCAATTGCAAAGCAAAAATGTAACACCCCTACAATGGATGCCGACAAGATTGTATTCAAAGTCAATAATCAGGTGGTCTCTTTGAAGCCTGAGATTATCGTGGATCAATATGAGTACAAGTACCCTGCCATCAAAGATTTGAAAAAGCCCTGTATAACCCTAGGAAAGGCTCCCGATTTAAATAAAGCATACAAGTCAGTTTTGTCAGGCATGAGCGCCGCCAAACTTAATCCTGACGATGTATGTTCCTATTTGGCAGCGGCAATGCAGTTTTTTGAGGGGACATGTCCGGAAGACTGGACCAGCTATGGAATTGTGATTGCACGAAAAGGAGATAAGATCACCCCAGGTTCTCTGGTGGAGATAAAACGTACTGATGTAGAAGGGAATTGGGCTCTGACAGGAGGCATGGAACTGACAAGAGACCCCACTGTCCCTGAGCATGCGTCCTTAGTCGGTCTTCTCTTGAGTCTGTATAGGTTGAGCAAAATATCCGGGCAAAACACTGGTAACTATAAGACAAACATTGCAGACAGGATAGAGCAGATTTTTGAGACAGCCCCTTTTGTTAAAATCGTGGAACACCATACTCTAATGACAACTCACAAaATGTGTGCTAATTGGAGTACTATACCAAACTTCAGATTTTTGGCCGGAACCTATGACATGTTTTTCTCCCGGATTGAGCATCTATATTCAGCAATCAGAGTGGGCACAGTTGTCACTGCTTATGAAGACTGTTCAGGACTGGTATCATTTACTGGGTTCATAAAACAAATCAATCTCACCGCTAGAGAGGCAATACTATATTTCTTCCACAAGAACTTTGAGGAAGAGATAAGAAGAATGTTTGAGCCAGGGCAGGAGACAGCTGTTCCTCACTCTTATTTCATCCACTTCCGTTCACTAGGCTTGAGTGGGAAATCTCCTTATTCATCAAATGCTGTTGGTCACGTGTTCAATCTCATTCACTTTGTAGGATGCTATATGGGTCAAGTCAGATCCCTAAATGCAACGGTTATTGCTGCATGTGCTCCTCATGAAATGTCTGTTCTAGGGGGCTATCTGGGAGAGGAATTCTTCGGGAAAGGGACATTTGAAAGAAGATTCTTCAGAGATGAGAAAGAACTTCAAGAATACGAGGCGGCTGAACTGACAAAGACTGACGTAGCACTGGCAGATGATGGAACTGTCAACTCTGACGACGAGGACTACTTTTCAGGTGAAACCAGAAGTCCGGAGGCTGTTTATACTCGAATCATGATGAATGGAGGTCGACTAAAGAGATCTCACATACGGAGATATGTCTCAGTCAGTTCCAATCATCAAGCCCGTCCAAACTCATTCGCCGAGTTTCTAAACAAGACATATTCGAGTGACTCATAAcatgaaaaaaactaacacccctcccGTACGCCACCATGTTCGTGTTTCTGGTGCTGCTGCCTCTGGTGAGCTCCCAGTGCGTGAACCTGACCACAAGGACCCAGCTGCCCCCTGCCTATACCAATTCCTTCACACGGGGCGTGTACTATCCCGACAAGGTGTTCCGGAGCAGCGTGCTGCACTCCACACAGGATCTGTTTCTGCCTTTCTTTTCTAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAATGGCACAAAGCGGTTCGACAATCCAGTGCTGCCCTTTAACGATGGCGTGTACTTCGCCTCCACCGAGAAGTCTAACATCATCAGAGGCTGGATCTTTGGCACCACACTGGACAGCAAGACACAGTCCCTGCTGATCGTGAACAATGCCACCAACGTGGTCATCAAGGTGTGCGAGTTCCAGTTTTGTAATGATCCATTCCTGGGCGTGTACTATCACAAGAACAATAAGTCTTGGATGGAGAGCGAGTTTCGCGTGTATTCCTCTGCCAACAATTGCACATTTGAGTACGTGTCCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAATTTCAAGAACCTGAGGGAGTTCGTGTTTAAGAATATCGATGGCTACTTCAAAATCTACTCCAAGCACACCCCAATCAACCTGGTGCGCGACCTGCCACAGGGCTTCTCTGCCCTGGAGCCACTGGTGGATCTGCCCATCGGCATCAACATCACCCGGTTTCAGACACTGCTGGCCCTGCACAGAAGCTACCTGACACCAGGCGACAGCTCCTCTGGATGGACCGCAGGAGCAGCAGCCTACTATGTGGGCTATCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAATGGCACCATCACAGACGCCGTGGATTGCGCCCTGGATCCCCTGTCTGAGACCAAGTGTACACTGAAGAGCTTTACCGTGGAGAAGGGCATCTATCAGACAAGCAATTTCAGGGTGCAGCCTACCGAGTCCATCGTGCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAAGATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGGAgGGCTTTAACTGTTATTTCCCTCTGCAGAgCTACgGCTTCCAGCCAACAAACGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACcTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGACTGACCGGAACAGGCGTGCTGACCGAGTCCAACAAGAAGTTCCTGCCTTTTCAGCAGTTCGGCAGGGACATCGCAGATACCACAGACGCCGTGCGCGACCCTCAGACCCTGGAGATCCTGGACATCACACCATGCTCCTTCGGCGGCGTGTCTGTGATCACACCAGGCACCAATACAAGCAACCAGGTGGCCGTGCTGTATCAGGACGTGAATTGTACCGAGGTGCCAGTGGCAATCCACGCAGATCAGCTGACCCCTACATGGCGGGTGTACTCTACCGGCAGCAACGTGTTCCAGACAAGAGCCGGATGCCTGATCGGAGCAGAGCACGTGAACAATAGCTATGAGTGCGACATCCCTATCGGCGCCGGCATCTGTGCCTCCTACCAGACCCAGACAAACTCCCCAAGGTCTGTGGGAGATGAGGCCGAAGACTTTGTGGAAGTCCACCTGCCTGATGTGCATAACCAGGTGTCTGGCGTCGACCTGGGACTGCCAAATTGGGGCAAGTACGTGCTGCTGAGTGCTGGAGCACTGACTGCCCTGATGCTGATCATTTTCCTGATGACCTGCTGTCGGCGCGTGAACAGAAGTGAGCCCACTCAGCACAATCTGCGAGGAACCGGGAGAGAAGTGTCAGTCACACCTCAGAGCGGGAAAATCATTAGTAGTTGGGAATCACATAAAAGCGGGGGCGAGACCAGGCTGTGAGCTAGCCATGAAAAAAACTAACACCCCTCCTTTCGAACCATCCCAAACATGAGCAAGATCTTTGTCAATCCTAGTGCTATTAGAGCCGGTCTGGCCGATCTTGAGATGGCTGAAGAAACTGTTGATCTGATCAATAGAAATATCGAAGACAATCAGGCTCATCTCCAAGGGGAACCCATAGAGGTGGACAATCTCCCTGAGGATATGGGGCGACTTCACCTGGATGATGGAAAATCGCCCAACCATGGTGAGATAGCCAAGGTGGGAGAAGGCAAGTATCGAGAGGACTTTCAGATGGATGAAGGAGAGGATCCTAGCTTCCTGTTCCAGTCATACCTGGAAAATGTTGGAGTCCAAATAGTCAGACAAATGAGGTCAGGAGAGAGATTTCTCAAGATATGGTCACAGACCGTAGAAGAGATTATATCCTATGTCGCGGTCAACTTTCCCAACCCTCCAGGAAAGTCTTCAGAGGATAAATCAACCCAGACTACTGGCCGAGAGCTCAAGAAGGAGACAACACCCACTCCTTCTCAGAGAGAAAGCCAATCATCGAAAGCCAGGATGGCGGCTCAAATTGCTTCTGGCCCTCCAGCCCTTGAATGGTCGGCTACCAATGAAGAGGATGATCTATCAGTGGAGGCTGAGATCGCTCACCAGATTGCAGAAAGTTTCTCCAAAAAATATAAGTTTCCCTCTCGATCCTCAGGGATACTCTTGTATAATTTTGAGCAATTGAAAATGAACCTTGATGATATAGTTAAAGAGGCAAAAAATGTACCAGGTGTGACCCGTTTAGCCCATGACGGGTCCAAACTCCCCCTAAGATGTGTACTGGGATGGGTCGCTTTGGCCAACTCTAAGAAATTCCAGTTGTTAGTCGAATCCGACAAGCTGAGTAAAATCATGCAAGATGACTTGAATCGCTATACATCTTGCTAACCGAACCTCTCCCCTCAGTCCCTCTAGACAATAAAATCCGAGATGTCCCAAAGTCAACATGAAAAAAACAGGCAACACCACTGATAAAATGAACCTCCTACGTAAGATAGTGAAAAACCGCAGGGACGAGGACACTCAAAAATCCTCTCCCGCGTCAGCCCCTCTGGATGACGATGACTTGTGGCTTCCACCCCCTGAATACGTCCCGCTGAAAGAACTTACAGGCAAGAAGAACATGAGGAACTTTTGTATCAACGGAAGGGTTAAAGTGTGTAGCCCGAATGGTTACTCGTTCAGGATCCTGCGGCACATTCTGAAATCATTCGACGAGATATATTCTGGGAATCATAGGATGATCGGGTTAGTCAAAGTGGTTATTGGACTGGCTTTGTCAGGATCTCCAGTCCCTGAGGGCCTGAACTGGGTATACAAATTGAGGAGAACCTTTATCTTCCAGTGGGCTGATTCCAGGGGCCCTCTTGAAGGGGAGGAGTTGGAATACTCTCAGGAGATCACTTGGGATGATGATACTGAGTTCGTCGGATTGCAAATAAGAGTGATTGCAAAACAGTGTCATATCCAGGGCAGAGTCTGGTGTATCAACATGAACCCGAGAGCATGTCAACTATGGTCTGACATGTCTCTTCAGACACAAAGGTCCGAAGAGGACAAAGATTCCTCTCTGCTTCTAGAATAATCAGATTATATCCCGCAAATTTATCACTTGTTTACCTCTGGAGGAGAGAACATATGGGCTCAACTCCAACCCTTGGGAGCAATATAACAAAAAACATGTTATGGTGCCATTAAACCGCTGCATTTCATCAAAGTCAAGTTGATTACCTTTACATTTTGATCCTCTTGGATGTGAAAAAAACTATTAACATCCCTCAAAAGACcccgggAAAGATGGTTCCTCAGGCTCTCCTGTTTGTACCCCTTCTGGTTTTTCCATTGTGTTTTGGGAAATTCCCTATTTACACGATACCAGACAAGCTTGGTCCCTGGAGTCCGATTGACATACATCACCTCAGCTGCCCAAACAATTTGGTAGTGGAGGACGAAGGATGCACCAACCTGTCAGGGTTCTCCTACATGGAACTTAAAGTTGGATACATCTTAGCCATAAAAGTGAACGGGTTCACTTGCACAGGCGTTGTGACGGAGGCTGAAACCTACACTAACTTCGTTGGTTATGTCACAACCACGTTCAAAAGAAAGCATTTCCGCCCAACACCAGATGCATGTAGAGCCGCGTACAACTGGAAGATGGCCGGTGACCCCAGATATGAAGAGTCTCTACACAATCCGTACCCTGACTACCGCTGGCTTCGAACTGTAAAAACCACCAAGGAGTCTCTCGTTATCATATCTCCAAGTGTGGCAGATTTGGACCCATATGACAGATCCCTTCACTCGAGGGTCTTCCCTAGCGGGAAGTGCTCAGGAGTAGCGGTGTCTTCTACCTACTGCTCCACTAACCACGATTACACCATTTGGATGCCCGAGAATCCGAGACTAGGGATGTCTTGTGACATTTTTACCAATAGTAGAGGGAAGAGAGCATCCAAAGGGAGTGAGACTTGCGGCTTTGTAGATGAAAGAGGCCTATATAAGTCTTTAAAAGGAGCATGCAAACTCAAGTTATGTGGAGTTCTAGGACTTAGACTTATGGATGGAACATGGGTCTCGATGCAAACATCAAATGAAACCAAATGGTGCCCTCCCGATAAGTTGGTGAACCTGCACGACTTTCGCTCAGACGAAATTGAGCACCTTGTTGTAGAGGAGTTGGTCAGGAAGAGAGAGGAGTGTCTGGATGCACTAGAGTCCATCATGACAACCAAGTCAGTGAGTTTCAGACGTCTCAGTCATTTAAGAAAACTTGTCCCTGGGTTTGGAAAAGCATATACCATATTCAACAAGACCTTGATGGAAGCCGATGCTCACTACAAGTCAGTCgagACTTGGAATGAGATCCTCCCTTCAAAAGGGTGTTTAAGAGTTGGGGGGAGGTGTCATCCTCATGTGAACGGGGTGTTTTTCAATGGTATAATATTAGGACCTGACGGCAATGTCTTAATCCCAGAGATGCAATCATCCCTCCTCCAGCAACATATGGAGTTGTTGGAATCCTCGGTTATCCCCCTTGTGCACCCCCTGGCAGACCCGTCTACCGTTTTCAAGGACGGTGACGAGGCTGAGGATTTTGTTGAAGTTCACCTTCCCGATGTGCACAATCAGGTCTCAGGAGTTGACTTGGGTCTCCCGAACTGGGGGAAGTATGTATTACTGAGTGCAGGGGCCCTGACTGCCTTGATGTTGATAATTTTCCTGATGACATGTTGTAGAAGAGTCAATCGATCAGAACCTACGCAACACAATCTCAGAGGGACAGGGAGGGAGGTGTCAGTCACTCCCCAAAGCGGGAAGATCATATCTTCATGGGAATCACACAAGAGTGGGGGTGAGACCAGACTGTAAttaattaaCGTCCTTTCAACGATCCAAGTCcatgaaaaaaactaacacccctcccgtacctagcTTATAAAGTGCTGGGTCATCTAAGCTTTTCAGTCGAGAAAAAAACATTAGATCAGAAGAACAACTGGCAACACTTCTCAACCTGAGACTTACTTCAAGATGCTCGATCCTGGAGAGGTCTATGATGACCCTATTGACCCAATCGAGTTAGAGGCTGAACCCAGAGGAACCCCCATTGTCCCCAACATCTTGAGGAACTCTGACTACAATCTCAACTCTCCTTTGATAGAAGATCCTGCTAGACTAATGTTAGAATGGTTAAAAACAGGGAATAGACCTTATCGGATGACTCTAACAGACAATTGCTCCAGGTCTTTCAGAGTTTTGAAAGATTATTTCAAGAAGGTAGATTTGGGTTCTCTCAAGGTGGGCGGAATGGCTGCACAGTCAATGATTTCTCTCTGGTTATATGGTGCCCACTCTGAATCCAACAGGAGCCGGAGATGTATAACAGACTTGGCCCATTTCTATTCCAAGTCGTCCCCCATAGAGAAGCTGTTGAATCTCACGCTAGGAAATAGAGGGCTGAGAATCCCCCCAGAGGGAGTGTTAAGTTGCCTTGAGAGGGTTGATTATGATAATGCATTTGGAAGGTATCTTGCCAACACGTATTCCTCTTACTTGTTCTTCCATGTAATCACCTTATACATGAACGCCCTAGACTGGGATGAAGAAAAGACCATCCTAGCATTATGGAAAGATTTAACCTCAGTGGACATCGGGAAGGACTTGGTAAAGTTCAAAGACCAAATATGGGGACTGCTGATCGTGACAAAGGACTTTGTTTACTCCCAAAGTTCCAATTGTCTTTTTGACAGAAACTACACACTTATGCTAAAAGATCTTTTCTTGTCTCGCTTCAACTCCTTAATGGTCTTGCTCTCTCCCCCAGAGCCCCGATACTCAGATGACTTGATATCTCAACTATGCCAGCTGTACATTGCTGGGGATCAAGTCTTGTCTATGTGTGGAAACTCCGGCTATGAAGTCATCAAAATATTGGAGCCATATGTCGTGAATAGTTTAGTCCAGAGAGCAGAAAAGTTTAGGCCTCTCATTCATTCCTTGGGAGACTTTCCTGTATTTATAAAAGACAAGGTAAGTCAACTTGAAGAGACGTTCGGTCCCTGTGCAAGAAGGTTCTTTAGGGCTCTGGATCAATTCGACAACATACATGACTTGGTTTTTGTGTTTGGCTGTTACAGGCATTGGGGGCACCCATATATAGATTATCGAAAGGGTCTGTCAAAACTATATGATCAGGTTCACCTTAAAAAAATGATAGATAAGTCCTACCAGGAGTGCTTAGCAAGCGACCTAGCCAGGAGGATCCTTAGATGGGGTTTTGATAAGTACTCCAAGTGGTATCTGGATTCAAGATTCCTAGCCCGAGACCACCCCTTGACTCCTTATATCAAAACCCAAACATGGCCACCCAAACATATTGTAGACTTGGTGGGGGATACATGGCACAAGCTCCCGATCACGCAGATCTTTGAGATTCCTGAATCAATGGATCCGTCAGAAATATTGGATGACAAATCACATTCTTTCACCAGAACGAGACTAGCTTCTTGGCTGTCAGAAAACCGAGGGGGGCCTGTTCCTAGCGAAAAAGTTATTATCACGGCCCTGTCTAAGCCGCCTGTCAATCCCCGAGAGTTTCTGAGGTCTATAGACCTCGGAGGATTGCCAGATGAAGACTTGATAATTGGCCTCAAGCCAAAGGAACGGGAATTGAAGATTGAAGGTCGATTCTTTGCTCTAATGTCATGGAATCTAAGATTGTATTTTGTCATCACTGAAAAACTCTTGGCCAACTACATCTTGCCACTTTTTGACGCGCTGACTATGACAGACAACCTGAACAAGGTGTTTAAAAAGCTGATCGACAGGGTCACCGGGCAAGGGCTTTTGGACTATTCAAGGGTCACATATGCATTTCACCTGGACTATGAAAAGTGGAACAACCATCAAAGATTAGAGTCAACAGAGGATGTATTTTCTGTCCTAGATCAAGTGTTTGGATTGAAGAGAGTGTTTTCTAGAACACACGAGTTTTTTCAAAAGGCCTGGATCTATTATTCAGACAGATCAGACCTCATCGGGTTACGGGAGGATCAAATATACTGCTTAGATGCGTCCAACGGCCCAACCTGTTGGAATGGCCAGGATGGCGGGCTAGAAGGCTTACGGCAGAAGGGCTGGAGTCTAGTCAGCTTATTGATGATAGATAGAGAATCTCAAATCAGGAACACAAGAACCAAAATACTAGCTCAAGGAGACAACCAGGTTTTATGTCCGACATACATGTTGTCGCCAGGGCTATCTCAAGAGGGGCTCCTCTATGAATTGGAGAGAATATCAAGGAATGCACTTTCGATATACAGAGCCGTCGAGGAAGGGGCATCTAAGCTAGGGCTGATCATCAAGAAAGAAGAGACCATGTGTAGTTATGACTTCCTCATCTATGGAAAAACCCCTTTGTTTAGAGGTAACATATTGGTGCCTGAGTCCAAAAGATGGGCCAGAGTCTCTTGCGTCTCTAATGACCAAATAGTCAACCTCGCCAATATAATGTCGACAGTGTCCACCAATGCGCTAACAGTGGCACAACACTCTCAATCTTTGATCAAACCGATGAGGGATTTTCTGCTCATGTCAGTACAGGCAGTCTTTCACTACCTGCTATTTAGCCCAATCTTAAAGGGAAGAGTTTACAAGATTCTGAGCGCTGAAGGGGAGAGCTTTCTCCTAGCCATGTCAAGGATAATCTATCTAGATCCTTCTTTGGGAGGGATATCTGGAATGTCCCTCGGAAGATTCCATATACGACAGTTCTCAGACCCTGTCTCTGAAGGGTTATCCTTCTGGAGAGAGATCTGGTTAAGCTCCCAAGAGTCCTGGATTCACGCGTTGTGTCAAGAGGCTGGAAACCCAGATCTTGGAGAGAGAACACTCGAGAGCTTCACTCGCCTTCTAGAAGATCCGACCACCTTAAATATCAGAGGAGGGGCCAGTCCTACCATTCTACTCAAGGATGCAATCAGAAAGGCTTTATATGACGAGGTGGACAAGGTGGAAAATTCAGAGTTTCGAGAGGCAATCCTGTTGTCCAAGACCCATAGAGATAATTTTATACTCTTCTTAATATCTGTTGAGCCTCTGTTTCCTCGATTTCTCAGTGAGCTATTCAGTTCGTCTTTTTTGGGAATCCCCGAGTCAATCATTGGATTGATACAAAACTCCCGAACGATAAGAAGGCAGTTTAGAAAGAGTCTCTCAAAAACTTTAGAAGAATCCTTCTACAACTCAGAGATCCACGGGATTAGTCGGATGACCCAGACACCTCAGAGGGTTGGGGGGGTGTGGCCTTGCTCTTCAGAGAGGGCAGATCTACTTAGGGAGATCTCTTGGGGAAGAAAAGTGGTAGGCACGACAGTTCCTCACCCTTCTGAGATGTTGGGATTACTTCCCAAGTCCTCTATTTCTTGCACTTGTGGAGCAACAGGAGGAGGCAATCCTAGAGTTTCTGTATCAGTACTCCCGTCCTTTGATCAGTCATTTTTTTCACGAGGCCCCCTAAAGGGATACTTGGGCTCGTCCACCTCTATGTCGACCCAGCTATTCCATGCATGGGAAAAAGTCACTAATGTTCATGTGGTGAAGAGAGCTCTATCGTTAAAAGAATCTATAAACTGGTTCATTACTAGAGATTCCAACTTGGCTCAAGCTCTAATTAGGAACATTATGTCTCTGACAGGCCCTGATTTCCCTCTAGAGGAGGCCCCTGTCTTCAAAAGGACGGGGTCAGCCTTGCATAGGTTCAAGTCTGCCAGATACAGCGAAGGAGGGTATTCTTCTGTCTGCCCGAACCTCCTCTCTCATATTTCTGTTAGTACAGACACCATGTCTGATTTGACCCAAGACGGGAAGAACTACGATTTCATGTTCCAGCCATTGATGCTTTATGCACAGACATGGACATCAGAGCTGGTACAGAGAGACACAAGGCTAAGAGACTCTACGTTTCATTGGCACCTCCGATGCAACAGGTGTGTGAGACCCATTGACGACGTGACCCTGGAGACCTCTCAGATCTTCGAGTTTCCGGATGTGTCGAAAAGAATATCCAGAATGGTTTCTGGGGCTGTGCCTCACTTCCAGAGGCTTCCCGATATCCGTCTGAGACCAGGAGATTTTGAATCTCTAAGCGGTAGAGAAAAGTCTCACCATATCGGATCAGCTCAGGGGCTCTTATACTCAATCTTAGTGGCAATTCACGACTCAGGATACAATGATGGAACCATCTTCCCTGTCAACATATACGGCAAGGTTTCCCCTAGAGACTATTTGAGAGGGCTCGCAAGGGGAGTATTGATAGGATCCTCGATTTGCTTCTTGACAAGAATGACAAATATCAATATTAATAGACCTCTTGAATTGGTCTCAGGGGTAATCTCATATATTCTCCTGAGGCTAGATAACCATCCCTCCTTGTACATAATGCTCAGAGAACCGTCTCTTAGAGGAGAGATATTTTCTATCCCTCAGAAAATCCCCGCCGCTTATCCAACCACTATGAAAGAAGGCAACAGATCAATCTTGTGTTATCTCCAACATGTGCTACGCTATGAGCGAGAGATAATCACGGCGTCTCCAGAGAATGACTGGCTATGGATCTTTTCAGACTTTAGAAGTGCCAAAATGACGTACCTATCCCTCATTACTTACCAGTCTCATCTTCTACTCCAGAGGGTTGAGAGAAACCTATCTAAGAGTATGAGAGATAACCTGCGACAATTGAGTTCTTTGATGAGGCAGGTGCTGGGCGGGCACGGAGAAGATACCTTAGAGTCAGACGACAACATTCAACGACTGCTAAAAGACTCTTTACGAAGGACAAGATGGGTGGATCAAGAGGTGCGCCATGCAGCTAGAACCATGACTGGAGATTACAGCCCCAACAAGAAGGTGTCCCGTAAGGTAGGATGTTCAGAATGGGTCTGCTCTGCTCAACAGGTTGCAGTCTCTACCTCAGCAAACCCGGCCCCTGTCTCGGAGCTTGACATAAGGGCCCTCTCTAAGAGGTTCCAGAACCCTTTGATCTCGGGCTTGAGAGTGGTTCAGTGGGCAACCGGTGCTCATTATAAGCTTAAGCCTATTCTAGATGATCTCAATGTTTTCCCATCTCTCTGCCTTGTAGTTGGGGACGGGTCAGGGGGGATATCAAGGGCAGTCCTCAACATGTTTCCAGATGCCAAGCTTGTGTTCAACAGTCTTTTAGAGGTGAATGACCTGATGGCTTCCGGAACACATCCACTGCCTCCTTCAGCAATCATGAGGGGAGGAAATGATATCGTCTCCAGAGTGATAGATCTTGACTCAATCTGGGAAAAACCGTCCGACTTGAGAAACTTGGCAACCTGGAAATACTTCCAGTCAGTCCAAAAGCAGGTCAACATGTCCTATGACCTCATTATTTGCGATGCAGAAGTTACTGACATTGCATCTATCAACCGGATCACCCTGTTAATGTCCGATTTTGCATTGTCTATAGATGGACCACTCTATTTGGTCTTCAAAACTTATGGGACTATGCTAGTAAATCCAAACTACAAGGCTATTCAACACCTGTCAAGAGCGTTCCCCTCGGTCACAGGGTTTATCACCCAAGTAACTTCGTCTTTTTCATCTGAGCTCTACCTCCGATTCTCCAAACGAGGGAAGTTTTTCAGAGATGCTGAGTACTTGACCTCTTCCACCCTTCGAGAAATGAGCCTTGTGTTATTCAATTGTAGCAGCCCCAAGAGTGAGATGCAGAGAGCTCGTTCCTTGAACTATCAGGATCTTGTGAGAGGATTTCCTGAAGAAATCATATCAAATCCTTACAATGAGATGATCATAACTCTGATTGACAGTGATGTAGAATCTTTTCTAGTCCACAAGATGGTTGATGATCTTGAGTTACAGAGGGGAACTCTGTCTAAAGTGGCTATCATTATAGCCATCATGATAGTTTTCTCCAACAGAGTCTTCAACGTTTCCAAACCCCTAACTGACCCCTCGTTCTATCCACCGTCTGATCCCAAAATCCTGAGGCACTTCAACATATGTTGCAGTACTATGATGTATCTATCTACTGCTTTAGGTGACGTCCCTAGCTTCGCAAGACTTCACGACCTGTATAACAGACCTATAACTTATTACTTCAGAAAGCAAGTCATTCGAGGGAACGTTTATCTATCTTGGAGTTGGTCCAACGACACCTCAGTGTTCAAAAGGGTAGCCTGTAATTCTAGCCTGAGTCTGTCATCTCACTGGATCAGGTTGATTTACAAGATAGTGAAGACTACCAGACTCGTTGGCAGCATCAAGGATCTATCCAGAGAAGTGGAAAGACACCTTCATAGGTACAACAGGTGGATCACCCTAGAGGATATCAGATCTAGATCATCCCTACTAGACTACAGTTGCCTGTGAACCGGATACTCCTGGAAGCCTGCCCATGCTAAGACTCTTGTGTGATGTATCTTGAAAAAAACAAGATCCTAAATCTGAACCTTTGGTTGTTTGATTGTTTTTCTCAtttttgttgtttatttgttaagcgt。
在另一个实施方式中，核酸包括SEQ ID NO：7的序列，如下所示(RABV载体：Coravax V1-South Africa(RABVG-E31))：
ACGCTTAACAACCAGATCAAAGAAAAAACAGACATTGTCAATTGCAAAGCAAAAATGTAACACCCCTACAATGGATGCCGACAAGATTGTATTCAAAGTCAATAATCAGGTGGTCTCTTTGAAGCCTGAGATTATCGTGGATCAATATGAGTACAAGTACCCTGCCATCAAAGATTTGAAAAAGCCCTGTATAACCCTAGGAAAGGCTCCCGATTTAAATAAAGCATACAAGTCAGTTTTGTCAGGCATGAGCGCCGCCAAACTTAATCCTGACGATGTATGTTCCTATTTGGCAGCGGCAATGCAGTTTTTTGAGGGGACATGTCCGGAAGACTGGACCAGCTATGGAATTGTGATTGCACGAAAAGGAGATAAGATCACCCCAGGTTCTCTGGTGGAGATAAAACGTACTGATGTAGAAGGGAATTGGGCTCTGACAGGAGGCATGGAACTGACAAGAGACCCCACTGTCCCTGAGCATGCGTCCTTAGTCGGTCTTCTCTTGAGTCTGTATAGGTTGAGCAAAATATCCGGGCAAAACACTGGTAACTATAAGACAAACATTGCAGACAGGATAGAGCAGATTTTTGAGACAGCCCCTTTTGTTAAAATCGTGGAACACCATACTCTAATGACAACTCACAAaATGTGTGCTAATTGGAGTACTATACCAAACTTCAGATTTTTGGCCGGAACCTATGACATGTTTTTCTCCCGGATTGAGCATCTATATTCAGCAATCAGAGTGGGCACAGTTGTCACTGCTTATGAAGACTGTTCAGGACTGGTATCATTTACTGGGTTCATAAAACAAATCAATCTCACCGCTAGAGAGGCAATACTATATTTCTTCCACAAGAACTTTGAGGAAGAGATAAGAAGAATGTTTGAGCCAGGGCAGGAGACAGCTGTTCCTCACTCTTATTTCATCCACTTCCGTTCACTAGGCTTGAGTGGGAAATCTCCTTATTCATCAAATGCTGTTGGTCACGTGTTCAATCTCATTCACTTTGTAGGATGCTATATGGGTCAAGTCAGATCCCTAAATGCAACGGTTATTGCTGCATGTGCTCCTCATGAAATGTCTGTTCTAGGGGGCTATCTGGGAGAGGAATTCTTCGGGAAAGGGACATTTGAAAGAAGATTCTTCAGAGATGAGAAAGAACTTCAAGAATACGAGGCGGCTGAACTGACAAAGACTGACGTAGCACTGGCAGATGATGGAACTGTCAACTCTGACGACGAGGACTACTTTTCAGGTGAAACCAGAAGTCCGGAGGCTGTTTATACTCGAATCATGATGAATGGAGGTCGACTAAAGAGATCTCACATACGGAGATATGTCTCAGTCAGTTCCAATCATCAAGCCCGTCCAAACTCATTCGCCGAGTTTCTAAACAAGACATATTCGAGTGACTCATAAcatgaaaaaaactaacacccctcccGTACGCCACCATGTTCGTGTTTCTGGTGCTGCTGCCTCTGGTGAGCTCCCAGTGCGTGAACTTCACCACAAGGACCCAGCTGCCCCCTGCCTATACCAATTCCTTCACACGGGGCGTGTACTATCCCGACAAGGTGTTCCGGAGCAGCGTGCTGCACTCCACACAGGATCTGTTTCTGCCTTTCTTTTCTAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAATGGCACAAAGCGGTTCGCCAATCCAGTGCTGCCCTTTAACGATGGCGTGTACTTCGCCTCCACCGAGAAGTCTAACATCATCAGAGGCTGGATCTTTGGCACCACACTGGACAGCAAGACACAGTCCCTGCTGATCGTGAACAATGCCACCAACGTGGTCATCAAGGTGTGCGAGTTCCAGTTTTGTAATGATCCATTCCTGGGCGTGTACTATCACAAGAACAATAAGTCTTGGATGGAGAGCGAGTTTCGCGTGTATTCCTCTGCCAACAATTGCACATTTGAGTACGTGTCCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAATTTCAAGAACCTGAGGGAGTTCGTGTTTAAGAATATCGATGGCTACTTCAAAATCTACTCCAAGCACACCCCAATCAACCTGGTGCGCGGCCTGCCACAGGGCTTCTCTGCCCTGGAGCCACTGGTGGATCTGCCCATCGGCATCAACATCACCCGGTTTCAGACACTGCTGGCCCTGCACAGAAGCTACCTGACACCAGGCGACAGCTCCTCTGGATGGACCGCAGGAGCAGCAGCCTACTATGTGGGCTATCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAATGGCACCATCACAGACGCCGTGGATTGCGCCCTGGATCCCCTGTCTGAGACCAAGTGTACACTGAAGAGCTTTACCGTGGAGAAGGGCATCTATCAGACAAGCAATTTCAGGGTGCAGCCTACCGAGTCCATCGTGCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAATATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGAAGGGCTTTAACTGTTATTTCCCTCTGCAGAGCTACGGCTTCCAGCCAACATATGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACCTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGACTGACCGGAACAGGCGTGCTGACCGAGTCCAACAAGAAGTTCCTGCCTTTTCAGCAGTTCGGCAGGGACATCGCAGATACCACAGACGCCGTGCGCGACCCTCAGACCCTGGAGATCCTGGACATCACACCATGCTCCTTCGGCGGCGTGTCTGTGATCACACCAGGCACCAATACAAGCAACCAGGTGGCCGTGCTGTATCAGGGCGTGAATTGTACCGAGGTGCCAGTGGCAATCCACGCAGATCAGCTGACCCCTACATGGCGGGTGTACTCTACCGGCAGCAACGTGTTCCAGACAAGAGCCGGATGCCTGATCGGAGCAGAGCACGTGAACAATAGCTATGAGTGCGACATCCCTATCGGCGCCGGCATCTGTGCCTCCTACCAGACCCAGACAAACTCCCCaagGTCTGTGGGAGATGAGGCCGAAGACTTTGTGGAAGTCCACCTGCCTGATGTGCATAACCAGGTGTCTGGCGTCGACCTGGGACTGCCAAATTGGGGCAAGTACGTGCTGCTGAGTGCTGGAGCACTGACTGCCCTGATGCTGATCATTTTCCTGATGACCTGCTGTCGGCGCGTGAACAGAAGTGAGCCCACTCAGCACAATCTGCGAGGAACCGGGAGAGAAGTGTCAGTCACACCTCAGAGCGGGAAAATCATTAGTAGTTGGGAATCACATAAAAGCGGGGGCGAGACCAGGCTGTGAGCTAGCCATGAAAAAAACTAACACCCCTCCTTTCGAACCATCCCAAACATGAGCAAGATCTTTGTCAATCCTAGTGCTATTAGAGCCGGTCTGGCCGATCTTGAGATGGCTGAAGAAACTGTTGATCTGATCAATAGAAATATCGAAGACAATCAGGCTCATCTCCAAGGGGAACCCATAGAGGTGGACAATCTCCCTGAGGATATGGGGCGACTTCACCTGGATGATGGAAAATCGCCCAACCATGGTGAGATAGCCAAGGTGGGAGAAGGCAAGTATCGAGAGGACTTTCAGATGGATGAAGGAGAGGATCCTAGCTTCCTGTTCCAGTCATACCTGGAAAATGTTGGAGTCCAAATAGTCAGACAAATGAGGTCAGGAGAGAGATTTCTCAAGATATGGTCACAGACCGTAGAAGAGATTATATCCTATGTCGCGGTCAACTTTCCCAACCCTCCAGGAAAGTCTTCAGAGGATAAATCAACCCAGACTACTGGCCGAGAGCTCAAGAAGGAGACAACACCCACTCCTTCTCAGAGAGAAAGCCAATCATCGAAAGCCAGGATGGCGGCTCAAATTGCTTCTGGCCCTCCAGCCCTTGAATGGTCGGCTACCAATGAAGAGGATGATCTATCAGTGGAGGCTGAGATCGCTCACCAGATTGCAGAAAGTTTCTCCAAAAAATATAAGTTTCCCTCTCGATCCTCAGGGATACTCTTGTATAATTTTGAGCAATTGAAAATGAACCTTGATGATATAGTTAAAGAGGCAAAAAATGTACCAGGTGTGACCCGTTTAGCCCATGACGGGTCCAAACTCCCCCTAAGATGTGTACTGGGATGGGTCGCTTTGGCCAACTCTAAGAAATTCCAGTTGTTAGTCGAATCCGACAAGCTGAGTAAAATCATGCAAGATGACTTGAATCGCTATACATCTTGCTAACCGAACCTCTCCCCTCAGTCCCTCTAGACAATAAAATCCGAGATGTCCCAAAGTCAACATGAAAAAAACAGGCAACACCACTGATAAAATGAACCTCCTACGTAAGATAGTGAAAAACCGCAGGGACGAGGACACTCAAAAATCCTCTCCCGCGTCAGCCCCTCTGGATGACGATGACTTGTGGCTTCCACCCCCTGAATACGTCCCGCTGAAAGAACTTACAGGCAAGAAGAACATGAGGAACTTTTGTATCAACGGAAGGGTTAAAGTGTGTAGCCCGAATGGTTACTCGTTCAGGATCCTGCGGCACATTCTGAAATCATTCGACGAGATATATTCTGGGAATCATAGGATGATCGGGTTAGTCAAAGTGGTTATTGGACTGGCTTTGTCAGGATCTCCAGTCCCTGAGGGCCTGAACTGGGTATACAAATTGAGGAGAACCTTTATCTTCCAGTGGGCTGATTCCAGGGGCCCTCTTGAAGGGGAGGAGTTGGAATACTCTCAGGAGATCACTTGGGATGATGATACTGAGTTCGTCGGATTGCAAATAAGAGTGATTGCAAAACAGTGTCATATCCAGGGCAGAGTCTGGTGTATCAACATGAACCCGAGAGCATGTCAACTATGGTCTGACATGTCTCTTCAGACACAAAGGTCCGAAGAGGACAAAGATTCCTCTCTGCTTCTAGAATAATCAGATTATATCCCGCAAATTTATCACTTGTTTACCTCTGGAGGAGAGAACATATGGGCTCAACTCCAACCCTTGGGAGCAATATAACAAAAAACATGTTATGGTGCCATTAAACCGCTGCATTTCATCAAAGTCAAGTTGATTACCTTTACATTTTGATCCTCTTGGATGTGAAAAAAACTATTAACATCCCTCAAAAGACcccgggAAAGATGGTTCCTCAGGCTCTCCTGTTTGTACCCCTTCTGGTTTTTCCATTGTGTTTTGGGAAATTCCCTATTTACACGATACCAGACAAGCTTGGTCCCTGGAGTCCGATTGACATACATCACCTCAGCTGCCCAAACAATTTGGTAGTGGAGGACGAAGGATGCACCAACCTGTCAGGGTTCTCCTACATGGAACTTAAAGTTGGATACATCTTAGCCATAAAAGTGAACGGGTTCACTTGCACAGGCGTTGTGACGGAGGCTGAAACCTACACTAACTTCGTTGGTTATGTCACAACCACGTTCAAAAGAAAGCATTTCCGCCCAACACCAGATGCATGTAGAGCCGCGTACAACTGGAAGATGGCCGGTGACCCCAGATATGAAGAGTCTCTACACAATCCGTACCCTGACTACCGCTGGCTTCGAACTGTAAAAACCACCAAGGAGTCTCTCGTTATCATATCTCCAAGTGTGGCAGATTTGGACCCATATGACAGATCCCTTCACTCGAGGGTCTTCCCTAGCGGGAAGTGCTCAGGAGTAGCGGTGTCTTCTACCTACTGCTCCACTAACCACGATTACACCATTTGGATGCCCGAGAATCCGAGACTAGGGATGTCTTGTGACATTTTTACCAATAGTAGAGGGAAGAGAGCATCCAAAGGGAGTGAGACTTGCGGCTTTGTAGATGAAAGAGGCCTATATAAGTCTTTAAAAGGAGCATGCAAACTCAAGTTATGTGGAGTTCTAGGACTTAGACTTATGGATGGAACATGGGTCTCGATGCAAACATCAAATGAAACCAAATGGTGCCCTCCCGATAAGTTGGTGAACCTGCACGACTTTCGCTCAGACGAAATTGAGCACCTTGTTGTAGAGGAGTTGGTCAGGAAGAGAGAGGAGTGTCTGGATGCACTAGAGTCCATCATGACAACCAAGTCAGTGAGTTTCAGACGTCTCAGTCATTTAAGAAAACTTGTCCCTGGGTTTGGAAAAGCATATACCATATTCAACAAGACCTTGATGGAAGCCGATGCTCACTACAAGTCAGTCgagACTTGGAATGAGATCCTCCCTTCAAAAGGGTGTTTAAGAGTTGGGGGGAGGTGTCATCCTCATGTGAACGGGGTGTTTTTCAATGGTATAATATTAGGACCTGACGGCAATGTCTTAATCCCAGAGATGCAATCATCCCTCCTCCAGCAACATATGGAGTTGTTGGAATCCTCGGTTATCCCCCTTGTGCACCCCCTGGCAGACCCGTCTACCGTTTTCAAGGACGGTGACGAGGCTGAGGATTTTGTTGAAGTTCACCTTCCCGATGTGCACAATCAGGTCTCAGGAGTTGACTTGGGTCTCCCGAACTGGGGGAAGTATGTATTACTGAGTGCAGGGGCCCTGACTGCCTTGATGTTGATAATTTTCCTGATGACATGTTGTAGAAGAGTCAATCGATCAGAACCTACGCAACACAATCTCAGAGGGACAGGGAGGGAGGTGTCAGTCACTCCCCAAAGCGGGAAGATCATATCTTCATGGGAATCACACAAGAGTGGGGGTGAGACCAGACTGTAAttaattaaCGTCCTTTCAACGATCCAAGTCcatgaaaaaaactaacacccctcccgtacctagcTTATAAAGTGCTGGGTCATCTAAGCTTTTCAGTCGAGAAAAAAACATTAGATCAGAAGAACAACTGGCAACACTTCTCAACCTGAGACTTACTTCAAGATGCTCGATCCTGGAGAGGTCTATGATGACCCTATTGACCCAATCGAGTTAGAGGCTGAACCCAGAGGAACCCCCATTGTCCCCAACATCTTGAGGAACTCTGACTACAATCTCAACTCTCCTTTGATAGAAGATCCTGCTAGACTAATGTTAGAATGGTTAAAAACAGGGAATAGACCTTATCGGATGACTCTAACAGACAATTGCTCCAGGTCTTTCAGAGTTTTGAAAGATTATTTCAAGAAGGTAGATTTGGGTTCTCTCAAGGTGGGCGGAATGGCTGCACAGTCAATGATTTCTCTCTGGTTATATGGTGCCCACTCTGAATCCAACAGGAGCCGGAGATGTATAACAGACTTGGCCCATTTCTATTCCAAGTCGTCCCCCATAGAGAAGCTGTTGAATCTCACGCTAGGAAATAGAGGGCTGAGAATCCCCCCAGAGGGAGTGTTAAGTTGCCTTGAGAGGGTTGATTATGATAATGCATTTGGAAGGTATCTTGCCAACACGTATTCCTCTTACTTGTTCTTCCATGTAATCACCTTATACATGAACGCCCTAGACTGGGATGAAGAAAAGACCATCCTAGCATTATGGAAAGATTTAACCTCAGTGGACATCGGGAAGGACTTGGTAAAGTTCAAAGACCAAATATGGGGACTGCTGATCGTGACAAAGGACTTTGTTTACTCCCAAAGTTCCAATTGTCTTTTTGACAGAAACTACACACTTATGCTAAAAGATCTTTTCTTGTCTCGCTTCAACTCCTTAATGGTCTTGCTCTCTCCCCCAGAGCCCCGATACTCAGATGACTTGATATCTCAACTATGCCAGCTGTACATTGCTGGGGATCAAGTCTTGTCTATGTGTGGAAACTCCGGCTATGAAGTCATCAAAATATTGGAGCCATATGTCGTGAATAGTTTAGTCCAGAGAGCAGAAAAGTTTAGGCCTCTCATTCATTCCTTGGGAGACTTTCCTGTATTTATAAAAGACAAGGTAAGTCAACTTGAAGAGACGTTCGGTCCCTGTGCAAGAAGGTTCTTTAGGGCTCTGGATCAATTCGACAACATACATGACTTGGTTTTTGTGTTTGGCTGTTACAGGCATTGGGGGCACCCATATATAGATTATCGAAAGGGTCTGTCAAAACTATATGATCAGGTTCACCTTAAAAAAATGATAGATAAGTCCTACCAGGAGTGCTTAGCAAGCGACCTAGCCAGGAGGATCCTTAGATGGGGTTTTGATAAGTACTCCAAGTGGTATCTGGATTCAAGATTCCTAGCCCGAGACCACCCCTTGACTCCTTATATCAAAACCCAAACATGGCCACCCAAACATATTGTAGACTTGGTGGGGGATACATGGCACAAGCTCCCGATCACGCAGATCTTTGAGATTCCTGAATCAATGGATCCGTCAGAAATATTGGATGACAAATCACATTCTTTCACCAGAACGAGACTAGCTTCTTGGCTGTCAGAAAACCGAGGGGGGCCTGTTCCTAGCGAAAAAGTTATTATCACGGCCCTGTCTAAGCCGCCTGTCAATCCCCGAGAGTTTCTGAGGTCTATAGACCTCGGAGGATTGCCAGATGAAGACTTGATAATTGGCCTCAAGCCAAAGGAACGGGAATTGAAGATTGAAGGTCGATTCTTTGCTCTAATGTCATGGAATCTAAGATTGTATTTTGTCATCACTGAAAAACTCTTGGCCAACTACATCTTGCCACTTTTTGACGCGCTGACTATGACAGACAACCTGAACAAGGTGTTTAAAAAGCTGATCGACAGGGTCACCGGGCAAGGGCTTTTGGACTATTCAAGGGTCACATATGCATTTCACCTGGACTATGAAAAGTGGAACAACCATCAAAGATTAGAGTCAACAGAGGATGTATTTTCTGTCCTAGATCAAGTGTTTGGATTGAAGAGAGTGTTTTCTAGAACACACGAGTTTTTTCAAAAGGCCTGGATCTATTATTCAGACAGATCAGACCTCATCGGGTTACGGGAGGATCAAATATACTGCTTAGATGCGTCCAACGGCCCAACCTGTTGGAATGGCCAGGATGGCGGGCTAGAAGGCTTACGGCAGAAGGGCTGGAGTCTAGTCAGCTTATTGATGATAGATAGAGAATCTCAAATCAGGAACACAAGAACCAAAATACTAGCTCAAGGAGACAACCAGGTTTTATGTCCGACATACATGTTGTCGCCAGGGCTATCTCAAGAGGGGCTCCTCTATGAATTGGAGAGAATATCAAGGAATGCACTTTCGATATACAGAGCCGTCGAGGAAGGGGCATCTAAGCTAGGGCTGATCATCAAGAAAGAAGAGACCATGTGTAGTTATGACTTCCTCATCTATGGAAAAACCCCTTTGTTTAGAGGTAACATATTGGTGCCTGAGTCCAAAAGATGGGCCAGAGTCTCTTGCGTCTCTAATGACCAAATAGTCAACCTCGCCAATATAATGTCGACAGTGTCCACCAATGCGCTAACAGTGGCACAACACTCTCAATCTTTGATCAAACCGATGAGGGATTTTCTGCTCATGTCAGTACAGGCAGTCTTTCACTACCTGCTATTTAGCCCAATCTTAAAGGGAAGAGTTTACAAGATTCTGAGCGCTGAAGGGGAGAGCTTTCTCCTAGCCATGTCAAGGATAATCTATCTAGATCCTTCTTTGGGAGGGATATCTGGAATGTCCCTCGGAAGATTCCATATACGACAGTTCTCAGACCCTGTCTCTGAAGGGTTATCCTTCTGGAGAGAGATCTGGTTAAGCTCCCAAGAGTCCTGGATTCACGCGTTGTGTCAAGAGGCTGGAAACCCAGATCTTGGAGAGAGAACACTCGAGAGCTTCACTCGCCTTCTAGAAGATCCGACCACCTTAAATATCAGAGGAGGGGCCAGTCCTACCATTCTACTCAAGGATGCAATCAGAAAGGCTTTATATGACGAGGTGGACAAGGTGGAAAATTCAGAGTTTCGAGAGGCAATCCTGTTGTCCAAGACCCATAGAGATAATTTTATACTCTTCTTAATATCTGTTGAGCCTCTGTTTCCTCGATTTCTCAGTGAGCTATTCAGTTCGTCTTTTTTGGGAATCCCCGAGTCAATCATTGGATTGATACAAAACTCCCGAACGATAAGAAGGCAGTTTAGAAAGAGTCTCTCAAAAACTTTAGAAGAATCCTTCTACAACTCAGAGATCCACGGGATTAGTCGGATGACCCAGACACCTCAGAGGGTTGGGGGGGTGTGGCCTTGCTCTTCAGAGAGGGCAGATCTACTTAGGGAGATCTCTTGGGGAAGAAAAGTGGTAGGCACGACAGTTCCTCACCCTTCTGAGATGTTGGGATTACTTCCCAAGTCCTCTATTTCTTGCACTTGTGGAGCAACAGGAGGAGGCAATCCTAGAGTTTCTGTATCAGTACTCCCGTCCTTTGATCAGTCATTTTTTTCACGAGGCCCCCTAAAGGGATACTTGGGCTCGTCCACCTCTATGTCGACCCAGCTATTCCATGCATGGGAAAAAGTCACTAATGTTCATGTGGTGAAGAGAGCTCTATCGTTAAAAGAATCTATAAACTGGTTCATTACTAGAGATTCCAACTTGGCTCAAGCTCTAATTAGGAACATTATGTCTCTGACAGGCCCTGATTTCCCTCTAGAGGAGGCCCCTGTCTTCAAAAGGACGGGGTCAGCCTTGCATAGGTTCAAGTCTGCCAGATACAGCGAAGGAGGGTATTCTTCTGTCTGCCCGAACCTCCTCTCTCATATTTCTGTTAGTACAGACACCATGTCTGATTTGACCCAAGACGGGAAGAACTACGATTTCATGTTCCAGCCATTGATGCTTTATGCACAGACATGGACATCAGAGCTGGTACAGAGAGACACAAGGCTAAGAGACTCTACGTTTCATTGGCACCTCCGATGCAACAGGTGTGTGAGACCCATTGACGACGTGACCCTGGAGACCTCTCAGATCTTCGAGTTTCCGGATGTGTCGAAAAGAATATCCAGAATGGTTTCTGGGGCTGTGCCTCACTTCCAGAGGCTTCCCGATATCCGTCTGAGACCAGGAGATTTTGAATCTCTAAGCGGTAGAGAAAAGTCTCACCATATCGGATCAGCTCAGGGGCTCTTATACTCAATCTTAGTGGCAATTCACGACTCAGGATACAATGATGGAACCATCTTCCCTGTCAACATATACGGCAAGGTTTCCCCTAGAGACTATTTGAGAGGGCTCGCAAGGGGAGTATTGATAGGATCCTCGATTTGCTTCTTGACAAGAATGACAAATATCAATATTAATAGACCTCTTGAATTGGTCTCAGGGGTAATCTCATATATTCTCCTGAGGCTAGATAACCATCCCTCCTTGTACATAATGCTCAGAGAACCGTCTCTTAGAGGAGAGATATTTTCTATCCCTCAGAAAATCCCCGCCGCTTATCCAACCACTATGAAAGAAGGCAACAGATCAATCTTGTGTTATCTCCAACATGTGCTACGCTATGAGCGAGAGATAATCACGGCGTCTCCAGAGAATGACTGGCTATGGATCTTTTCAGACTTTAGAAGTGCCAAAATGACGTACCTATCCCTCATTACTTACCAGTCTCATCTTCTACTCCAGAGGGTTGAGAGAAACCTATCTAAGAGTATGAGAGATAACCTGCGACAATTGAGTTCTTTGATGAGGCAGGTGCTGGGCGGGCACGGAGAAGATACCTTAGAGTCAGACGACAACATTCAACGACTGCTAAAAGACTCTTTACGAAGGACAAGATGGGTGGATCAAGAGGTGCGCCATGCAGCTAGAACCATGACTGGAGATTACAGCCCCAACAAGAAGGTGTCCCGTAAGGTAGGATGTTCAGAATGGGTCTGCTCTGCTCAACAGGTTGCAGTCTCTACCTCAGCAAACCCGGCCCCTGTCTCGGAGCTTGACATAAGGGCCCTCTCTAAGAGGTTCCAGAACCCTTTGATCTCGGGCTTGAGAGTGGTTCAGTGGGCAACCGGTGCTCATTATAAGCTTAAGCCTATTCTAGATGATCTCAATGTTTTCCCATCTCTCTGCCTTGTAGTTGGGGACGGGTCAGGGGGGATATCAAGGGCAGTCCTCAACATGTTTCCAGATGCCAAGCTTGTGTTCAACAGTCTTTTAGAGGTGAATGACCTGATGGCTTCCGGAACACATCCACTGCCTCCTTCAGCAATCATGAGGGGAGGAAATGATATCGTCTCCAGAGTGATAGATCTTGACTCAATCTGGGAAAAACCGTCCGACTTGAGAAACTTGGCAACCTGGAAATACTTCCAGTCAGTCCAAAAGCAGGTCAACATGTCCTATGACCTCATTATTTGCGATGCAGAAGTTACTGACATTGCATCTATCAACCGGATCACCCTGTTAATGTCCGATTTTGCATTGTCTATAGATGGACCACTCTATTTGGTCTTCAAAACTTATGGGACTATGCTAGTAAATCCAAACTACAAGGCTATTCAACACCTGTCAAGAGCGTTCCCCTCGGTCACAGGGTTTATCACCCAAGTAACTTCGTCTTTTTCATCTGAGCTCTACCTCCGATTCTCCAAACGAGGGAAGTTTTTCAGAGATGCTGAGTACTTGACCTCTTCCACCCTTCGAGAAATGAGCCTTGTGTTATTCAATTGTAGCAGCCCCAAGAGTGAGATGCAGAGAGCTCGTTCCTTGAACTATCAGGATCTTGTGAGAGGATTTCCTGAAGAAATCATATCAAATCCTTACAATGAGATGATCATAACTCTGATTGACAGTGATGTAGAATCTTTTCTAGTCCACAAGATGGTTGATGATCTTGAGTTACAGAGGGGAACTCTGTCTAAAGTGGCTATCATTATAGCCATCATGATAGTTTTCTCCAACAGAGTCTTCAACGTTTCCAAACCCCTAACTGACCCCTCGTTCTATCCACCGTCTGATCCCAAAATCCTGAGGCACTTCAACATATGTTGCAGTACTATGATGTATCTATCTACTGCTTTAGGTGACGTCCCTAGCTTCGCAAGACTTCACGACCTGTATAACAGACCTATAACTTATTACTTCAGAAAGCAAGTCATTCGAGGGAACGTTTATCTATCTTGGAGTTGGTCCAACGACACCTCAGTGTTCAAAAGGGTAGCCTGTAATTCTAGCCTGAGTCTGTCATCTCACTGGATCAGGTTGATTTACAAGATAGTGAAGACTACCAGACTCGTTGGCAGCATCAAGGATCTATCCAGAGAAGTGGAAAGACACCTTCATAGGTACAACAGGTGGATCACCCTAGAGGATATCAGATCTAGATCATCCCTACTAGACTACAGTTGCCTGTGAACCGGATACTCCTGGAAGCCTGCCCATGCTAAGACTCTTGTGTGATGTATCTTGAAAAAAACAAGATCCTAAATCTGAACCTTTGGTTGTTTGATTGTTTTTCTCAtttttgttgtttatttgttaagcgt。
在一些实施方式中，核酸包括SEQ ID NO：8的序列，如下所示(RABV载体：CoravaxV2-China(RABVG-E51))：ACGCTTAACAACCAGATCAAAGAAAAAACAGACATTGTCAATTGCAAAGCAAAAATGTAACACCCCTACAATGGATGCCGACAAGATTGTATTCAAAGTCAATAATCAGGTGGTCTCTTTGAAGCCTGAGATTATCGTGGATCAATATGAGTACAAGTACCCTGCCATCAAAGATTTGAAAAAGCCCTGTATAACCCTAGGAAAGGCTCCCGATTTAAATAAAGCATACAAGTCAGTTTTGTCAGGCATGAGCGCCGCCAAACTTAATCCTGACGATGTATGTTCCTATTTGGCAGCGGCAATGCAGTTTTTTGAGGGGACATGTCCGGAAGACTGGACCAGCTATGGAATTGTGATTGCACGAAAAGGAGATAAGATCACCCCAGGTTCTCTGGTGGAGATAAAACGTACTGATGTAGAAGGGAATTGGGCTCTGACAGGAGGCATGGAACTGACAAGAGACCCCACTGTCCCTGAGCATGCGTCCTTAGTCGGTCTTCTCTTGAGTCTGTATAGGTTGAGCAAAATATCCGGGCAAAACACTGGTAACTATAAGACAAACATTGCAGACAGGATAGAGCAGATTTTTGAGACAGCCCCTTTTGTTAAAATCGTGGAACACCATACTCTAATGACAACTCACAAaATGTGTGCTAATTGGAGTACTATACCAAACTTCAGATTTTTGGCCGGAACCTATGACATGTTTTTCTCCCGGATTGAGCATCTATATTCAGCAATCAGAGTGGGCACAGTTGTCACTGCTTATGAAGACTGTTCAGGACTGGTATCATTTACTGGGTTCATAAAACAAATCAATCTCACCGCTAGAGAGGCAATACTATATTTCTTCCACAAGAACTTTGAGGAAGAGATAAGAAGAATGTTTGAGCCAGGGCAGGAGACAGCTGTTCCTCACTCTTATTTCATCCACTTCCGTTCACTAGGCTTGAGTGGGAAATCTCCTTATTCATCAAATGCTGTTGGTCACGTGTTCAATCTCATTCACTTTGTAGGATGCTATATGGGTCAAGTCAGATCCCTAAATGCAACGGTTATTGCTGCATGTGCTCCTCATGAAATGTCTGTTCTAGGGGGCTATCTGGGAGAGGAATTCTTCGGGAAAGGGACATTTGAAAGAAGATTCTTCAGAGATGAGAAAGAACTTCAAGAATACGAGGCGGCTGAACTGACAAAGACTGACGTAGCACTGGCAGATGATGGAACTGTCAACTCTGACGACGAGGACTACTTTTCAGGTGAAACCAGAAGTCCGGAGGCTGTTTATACTCGAATCATGATGAATGGAGGTCGACTAAAGAGATCTCACATACGGAGATATGTCTCAGTCAGTTCCAATCATCAAGCCCGTCCAAACTCATTCGCCGAGTTTCTAAACAAGACATATTCGAGTGACTCATAAcatgaaaaaaactaacacccctcccgtacgCCACCATGTTCGTGTTTCTGGTGCTGCTGCCTCTGGTGAGCTCCCAGTGCGTGAACCTGACCACAAGGACCCAGCTGCCCCCTGCCTATACCAATTCCTTCACACGGGGCGTGTACTATCCCGACAAGGTGTTCCGGAGCAGCGTGCTGCACTCCACACAGGATCTGTTTCTGCCTTTCTTTTCTAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAATGGCACAAAGCGGTTCGACAATCCAGTGCTGCCCTTTAACGATGGCGTGTACTTCGCCTCCACCGAGAAGTCTAACATCATCAGAGGCTGGATCTTTGGCACCACACTGGACAGCAAGACACAGTCCCTGCTGATCGTGAACAATGCCACCAACGTGGTCATCAAGGTGTGCGAGTTCCAGTTTTGTAATGATCCATTCCTGGGCGTGTACTATCACAAGAACAATAAGTCTTGGATGGAGAGCGAGTTTCGCGTGTATTCCTCTGCCAACAATTGCACATTTGAGTACGTGTCCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAATTTCAAGAACCTGAGGGAGTTCGTGTTTAAGAATATCGATGGCTACTTCAAAATCTACTCCAAGCACACCCCAATCAACCTGGTGCGCGACCTGCCACAGGGCTTCTCTGCCCTGGAGCCACTGGTGGATCTGCCCATCGGCATCAACATCACCCGGTTTCAGACACTGCTGGCCCTGCACAGAAGCTACCTGACACCAGGCGACAGCTCCTCTGGATGGACCGCAGGAGCAGCAGCCTACTATGTGGGCTATCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAATGGCACCATCACAGACGCCGTGGATTGCGCCCTGGATCCCCTGTCTGAGACCAAGTGTACACTGAAGAGCTTTACCGTGGAGAAGGGCATCTATCAGACAAGCAATTTCAGGGTGCAGCCTACCGAGTCCATCGTGCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAAGATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGGAGGGCTTTAACTGTTATTTCCCTCTGCAGAGCTACGGCTTCCAGCCAACAAACGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACCTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGACTGACCGGAACAGGCGTGCTGACCGAGTCCAACAAGAAGTTCCTGCCTTTTCAGCAGTTCGGCAGGGACATCGCAGATACCACAGACGCCGTGCGCGACCCTCAGACCCTGGAGATCCTGGACATCACACCATGCTCCTTCGGCGGCGTGTCTGTGATCACACCAGGCACCAATACAAGCAACCAGGTGGCCGTGCTGTATcAGGACGTGAATTGTACCGAGGTGCCAGTGGCAATCCACGCAGATCAGCTGACCCCTACATGGCGGGTGTACTCTACCGGCAGCAACGTGTTCCAGACAAGAGCCGGATGCCTGATCGGAGCAGAGCACGTGAACAATAGCTATGAGTGCGACATCCCTATCGGCGCCGGCATCTGTGCCTCCTACCAGACCCAGACAAACTCCCCAGAATCAAGCGTGATTCCTCTGGTCCATCCACTGGCAGATCCCTCCACAGTGTTCAAAGACGGAGATGAGGCCGAAGACTTTGTGGAAGTCCACCTGCCTGATGTGCATAACCAGGTGTCTGGCGTCGACCTGGGACTGCCAAATTGGGGCAAGTACGTGCTGCTGAGTGCTGGAGCACTGACTGCCCTGATGCTGATCATTTTCCTGATGACCTGCTGTCGGCGCGTGAACAGAAGTGAGCCCACTCAGCACAATCTGCGAGGAACCGGGAGAGAAGTGTCAGTCACACCTCAGAGCGGGAAAATCATTAGTAGTTGGGAATCACATAAAAGCGGGGGCGAGACCAGGCTGTGAgctagcCATGAAAAAAACTAACACCCCTCCTTTCGAACCATCCCAAACATGAGCAAGATCTTTGTCAATCCTAGTGCTATTAGAGCCGGTCTGGCCGATCTTGAGATGGCTGAAGAAACTGTTGATCTGATCAATAGAAATATCGAAGACAATCAGGCTCATCTCCAAGGGGAACCCATAGAGGTGGACAATCTCCCTGAGGATATGGGGCGACTTCACCTGGATGATGGAAAATCGCCCAACCATGGTGAGATAGCCAAGGTGGGAGAAGGCAAGTATCGAGAGGACTTTCAGATGGATGAAGGAGAGGATCCTAGCTTCCTGTTCCAGTCATACCTGGAAAATGTTGGAGTCCAAATAGTCAGACAAATGAGGTCAGGAGAGAGATTTCTCAAGATATGGTCACAGACCGTAGAAGAGATTATATCCTATGTCGCGGTCAACTTTCCCAACCCTCCAGGAAAGTCTTCAGAGGATAAATCAACCCAGACTACTGGCCGAGAGCTCAAGAAGGAGACAACACCCACTCCTTCTCAGAGAGAAAGCCAATCATCGAAAGCCAGGATGGCGGCTCAAATTGCTTCTGGCCCTCCAGCCCTTGAATGGTCGGCTACCAATGAAGAGGATGATCTATCAGTGGAGGCTGAGATCGCTCACCAGATTGCAGAAAGTTTCTCCAAAAAATATAAGTTTCCCTCTCGATCCTCAGGGATACTCTTGTATAATTTTGAGCAATTGAAAATGAACCTTGATGATATAGTTAAAGAGGCAAAAAATGTACCAGGTGTGACCCGTTTAGCCCATGACGGGTCCAAACTCCCCCTAAGATGTGTACTGGGATGGGTCGCTTTGGCCAACTCTAAGAAATTCCAGTTGTTAGTCGAATCCGACAAGCTGAGTAAAATCATGCAAGATGACTTGAATCGCTATACATCTTGCTAACCGAACCTCTCCCCTCAGTCCCTCTAGACAATAAAATCCGAGATGTCCCAAAGTCAACATGAAAAAAACAGGCAACACCACTGATAAAATGAACCTCCTACGTAAGATAGTGAAAAACCGCAGGGACGAGGACACTCAAAAATCCTCTCCCGCGTCAGCCCCTCTGGATGACGATGACTTGTGGCTTCCACCCCCTGAATACGTCCCGCTGAAAGAACTTACAGGCAAGAAGAACATGAGGAACTTTTGTATCAACGGAAGGGTTAAAGTGTGTAGCCCGAATGGTTACTCGTTCAGGATCCTGCGGCACATTCTGAAATCATTCGACGAGATATATTCTGGGAATCATAGGATGATCGGGTTAGTCAAAGTGGTTATTGGACTGGCTTTGTCAGGATCTCCAGTCCCTGAGGGCCTGAACTGGGTATACAAATTGAGGAGAACCTTTATCTTCCAGTGGGCTGATTCCAGGGGCCCTCTTGAAGGGGAGGAGTTGGAATACTCTCAGGAGATCACTTGGGATGATGATACTGAGTTCGTCGGATTGCAAATAAGAGTGATTGCAAAACAGTGTCATATCCAGGGCAGAGTCTGGTGTATCAACATGAACCCGAGAGCATGTCAACTATGGTCTGACATGTCTCTTCAGACACAAAGGTCCGAAGAGGACAAAGATTCCTCTCTGCTTCTAGAATAATCAGATTATATCCCGCAAATTTATCACTTGTTTACCTCTGGAGGAGAGAACATATGGGCTCAACTCCAACCCTTGGGAGCAATATAACAAAAAACATGTTATGGTGCCATTAAACCGCTGCATTTCATCAAAGTCAAGTTGATTACCTTTACATTTTGATCCTCTTGGATGTGAAAAAAACTATTAACATCCCTCAAAAGACcccgggAAAGATGGTTCCTCAGGCTCTCCTGTTTGTACCCCTTCTGGTTTTTCCATTGTGTTTTGGGAAATTCCCTATTTACACGATACCAGACAAGCTTGGTCCCTGGAGTCCGATTGACATACATCACCTCAGCTGCCCAAACAATTTGGTAGTGGAGGACGAAGGATGCACCAACCTGTCAGGGTTCTCCTACATGGAACTTAAAGTTGGATACATCTTAGCCATAAAAGTGAACGGGTTCACTTGCACAGGCGTTGTGACGGAGGCTGAAACCTACACTAACTTCGTTGGTTATGTCACAACCACGTTCAAAAGAAAGCATTTCCGCCCAACACCAGATGCATGTAGAGCCGCGTACAACTGGAAGATGGCCGGTGACCCCAGATATGAAGAGTCTCTACACAATCCGTACCCTGACTACCGCTGGCTTCGAACTGTAAAAACCACCAAGGAGTCTCTCGTTATCATATCTCCAAGTGTGGCAGATTTGGACCCATATGACAGATCCCTTCACTCGAGGGTCTTCCCTAGCGGGAAGTGCTCAGGAGTAGCGGTGTCTTCTACCTACTGCTCCACTAACCACGATTACACCATTTGGATGCCCGAGAATCCGAGACTAGGGATGTCTTGTGACATTTTTACCAATAGTAGAGGGAAGAGAGCATCCAAAGGGAGTGAGACTTGCGGCTTTGTAGATGAAAGAGGCCTATATAAGTCTTTAAAAGGAGCATGCAAACTCAAGTTATGTGGAGTTCTAGGACTTAGACTTATGGATGGAACATGGGTCTCGATGCAAACATCAAATGAAACCAAATGGTGCCCTCCCGATAAGTTGGTGAACCTGCACGACTTTCGCTCAGACGAAATTGAGCACCTTGTTGTAGAGGAGTTGGTCAGGAAGAGAGAGGAGTGTCTGGATGCACTAGAGTCCATCATGACAACCAAGTCAGTGAGTTTCAGACGTCTCAGTCATTTAAGAAAACTTGTCCCTGGGTTTGGAAAAGCATATACCATATTCAACAAGACCTTGATGGAAGCCGATGCTCACTACAAGTCAGTCgagACTTGGAATGAGATCCTCCCTTCAAAAGGGTGTTTAAGAGTTGGGGGGAGGTGTCATCCTCATGTGAACGGGGTGTTTTTCAATGGTATAATATTAGGACCTGACGGCAATGTCTTAATCCCAGAGATGCAATCATCCCTCCTCCAGCAACATATGGAGTTGTTGGAATCCTCGGTTATCCCCCTTGTGCACCCCCTGGCAGACCCGTCTACCGTTTTCAAGGACGGTGACGAGGCTGAGGATTTTGTTGAAGTTCACCTTCCCGATGTGCACAATCAGGTCTCAGGAGTTGACTTGGGTCTCCCGAACTGGGGGAAGTATGTATTACTGAGTGCAGGGGCCCTGACTGCCTTGATGTTGATAATTTTCCTGATGACATGTTGTAGAAGAGTCAATCGATCAGAACCTACGCAACACAATCTCAGAGGGACAGGGAGGGAGGTGTCAGTCACTCCCCAAAGCGGGAAGATCATATCTTCATGGGAATCACACAAGAGTGGGGGTGAGACCAGACTGTAAttaattaaCGTCCTTTCAACGATCCAAGTCcatgaaaaaaactaacacccctcccgtacctagcTTATAAAGTGCTGGGTCATCTAAGCTTTTCAGTCGAGAAAAAAACATTAGATCAGAAGAACAACTGGCAACACTTCTCAACCTGAGACTTACTTCAAGATGCTCGATCCTGGAGAGGTCTATGATGACCCTATTGACCCAATCGAGTTAGAGGCTGAACCCAGAGGAACCCCCATTGTCCCCAACATCTTGAGGAACTCTGACTACAATCTCAACTCTCCTTTGATAGAAGATCCTGCTAGACTAATGTTAGAATGGTTAAAAACAGGGAATAGACCTTATCGGATGACTCTAACAGACAATTGCTCCAGGTCTTTCAGAGTTTTGAAAGATTATTTCAAGAAGGTAGATTTGGGTTCTCTCAAGGTGGGCGGAATGGCTGCACAGTCAATGATTTCTCTCTGGTTATATGGTGCCCACTCTGAATCCAACAGGAGCCGGAGATGTATAACAGACTTGGCCCATTTCTATTCCAAGTCGTCCCCCATAGAGAAGCTGTTGAATCTCACGCTAGGAAATAGAGGGCTGAGAATCCCCCCAGAGGGAGTGTTAAGTTGCCTTGAGAGGGTTGATTATGATAATGCATTTGGAAGGTATCTTGCCAACACGTATTCCTCTTACTTGTTCTTCCATGTAATCACCTTATACATGAACGCCCTAGACTGGGATGAAGAAAAGACCATCCTAGCATTATGGAAAGATTTAACCTCAGTGGACATCGGGAAGGACTTGGTAAAGTTCAAAGACCAAATATGGGGACTGCTGATCGTGACAAAGGACTTTGTTTACTCCCAAAGTTCCAATTGTCTTTTTGACAGAAACTACACACTTATGCTAAAAGATCTTTTCTTGTCTCGCTTCAACTCCTTAATGGTCTTGCTCTCTCCCCCAGAGCCCCGATACTCAGATGACTTGATATCTCAACTATGCCAGCTGTACATTGCTGGGGATCAAGTCTTGTCTATGTGTGGAAACTCCGGCTATGAAGTCATCAAAATATTGGAGCCATATGTCGTGAATAGTTTAGTCCAGAGAGCAGAAAAGTTTAGGCCTCTCATTCATTCCTTGGGAGACTTTCCTGTATTTATAAAAGACAAGGTAAGTCAACTTGAAGAGACGTTCGGTCCCTGTGCAAGAAGGTTCTTTAGGGCTCTGGATCAATTCGACAACATACATGACTTGGTTTTTGTGTTTGGCTGTTACAGGCATTGGGGGCACCCATATATAGATTATCGAAAGGGTCTGTCAAAACTATATGATCAGGTTCACCTTAAAAAAATGATAGATAAGTCCTACCAGGAGTGCTTAGCAAGCGACCTAGCCAGGAGGATCCTTAGATGGGGTTTTGATAAGTACTCCAAGTGGTATCTGGATTCAAGATTCCTAGCCCGAGACCACCCCTTGACTCCTTATATCAAAACCCAAACATGGCCACCCAAACATATTGTAGACTTGGTGGGGGATACATGGCACAAGCTCCCGATCACGCAGATCTTTGAGATTCCTGAATCAATGGATCCGTCAGAAATATTGGATGACAAATCACATTCTTTCACCAGAACGAGACTAGCTTCTTGGCTGTCAGAAAACCGAGGGGGGCCTGTTCCTAGCGAAAAAGTTATTATCACGGCCCTGTCTAAGCCGCCTGTCAATCCCCGAGAGTTTCTGAGGTCTATAGACCTCGGAGGATTGCCAGATGAAGACTTGATAATTGGCCTCAAGCCAAAGGAACGGGAATTGAAGATTGAAGGTCGATTCTTTGCTCTAATGTCATGGAATCTAAGATTGTATTTTGTCATCACTGAAAAACTCTTGGCCAACTACATCTTGCCACTTTTTGACGCGCTGACTATGACAGACAACCTGAACAAGGTGTTTAAAAAGCTGATCGACAGGGTCACCGGGCAAGGGCTTTTGGACTATTCAAGGGTCACATATGCATTTCACCTGGACTATGAAAAGTGGAACAACCATCAAAGATTAGAGTCAACAGAGGATGTATTTTCTGTCCTAGATCAAGTGTTTGGATTGAAGAGAGTGTTTTCTAGAACACACGAGTTTTTTCAAAAGGCCTGGATCTATTATTCAGACAGATCAGACCTCATCGGGTTACGGGAGGATCAAATATACTGCTTAGATGCGTCCAACGGCCCAACCTGTTGGAATGGCCAGGATGGCGGGCTAGAAGGCTTACGGCAGAAGGGCTGGAGTCTAGTCAGCTTATTGATGATAGATAGAGAATCTCAAATCAGGAACACAAGAACCAAAATACTAGCTCAAGGAGACAACCAGGTTTTATGTCCGACATACATGTTGTCGCCAGGGCTATCTCAAGAGGGGCTCCTCTATGAATTGGAGAGAATATCAAGGAATGCACTTTCGATATACAGAGCCGTCGAGGAAGGGGCATCTAAGCTAGGGCTGATCATCAAGAAAGAAGAGACCATGTGTAGTTATGACTTCCTCATCTATGGAAAAACCCCTTTGTTTAGAGGTAACATATTGGTGCCTGAGTCCAAAAGATGGGCCAGAGTCTCTTGCGTCTCTAATGACCAAATAGTCAACCTCGCCAATATAATGTCGACAGTGTCCACCAATGCGCTAACAGTGGCACAACACTCTCAATCTTTGATCAAACCGATGAGGGATTTTCTGCTCATGTCAGTACAGGCAGTCTTTCACTACCTGCTATTTAGCCCAATCTTAAAGGGAAGAGTTTACAAGATTCTGAGCGCTGAAGGGGAGAGCTTTCTCCTAGCCATGTCAAGGATAATCTATCTAGATCCTTCTTTGGGAGGGATATCTGGAATGTCCCTCGGAAGATTCCATATACGACAGTTCTCAGACCCTGTCTCTGAAGGGTTATCCTTCTGGAGAGAGATCTGGTTAAGCTCCCAAGAGTCCTGGATTCACGCGTTGTGTCAAGAGGCTGGAAACCCAGATCTTGGAGAGAGAACACTCGAGAGCTTCACTCGCCTTCTAGAAGATCCGACCACCTTAAATATCAGAGGAGGGGCCAGTCCTACCATTCTACTCAAGGATGCAATCAGAAAGGCTTTATATGACGAGGTGGACAAGGTGGAAAATTCAGAGTTTCGAGAGGCAATCCTGTTGTCCAAGACCCATAGAGATAATTTTATACTCTTCTTAATATCTGTTGAGCCTCTGTTTCCTCGATTTCTCAGTGAGCTATTCAGTTCGTCTTTTTTGGGAATCCCCGAGTCAATCATTGGATTGATACAAAACTCCCGAACGATAAGAAGGCAGTTTAGAAAGAGTCTCTCAAAAACTTTAGAAGAATCCTTCTACAACTCAGAGATCCACGGGATTAGTCGGATGACCCAGACACCTCAGAGGGTTGGGGGGGTGTGGCCTTGCTCTTCAGAGAGGGCAGATCTACTTAGGGAGATCTCTTGGGGAAGAAAAGTGGTAGGCACGACAGTTCCTCACCCTTCTGAGATGTTGGGATTACTTCCCAAGTCCTCTATTTCTTGCACTTGTGGAGCAACAGGAGGAGGCAATCCTAGAGTTTCTGTATCAGTACTCCCGTCCTTTGATCAGTCATTTTTTTCACGAGGCCCCCTAAAGGGATACTTGGGCTCGTCCACCTCTATGTCGACCCAGCTATTCCATGCATGGGAAAAAGTCACTAATGTTCATGTGGTGAAGAGAGCTCTATCGTTAAAAGAATCTATAAACTGGTTCATTACTAGAGATTCCAACTTGGCTCAAGCTCTAATTAGGAACATTATGTCTCTGACAGGCCCTGATTTCCCTCTAGAGGAGGCCCCTGTCTTCAAAAGGACGGGGTCAGCCTTGCATAGGTTCAAGTCTGCCAGATACAGCGAAGGAGGGTATTCTTCTGTCTGCCCGAACCTCCTCTCTCATATTTCTGTTAGTACAGACACCATGTCTGATTTGACCCAAGACGGGAAGAACTACGATTTCATGTTCCAGCCATTGATGCTTTATGCACAGACATGGACATCAGAGCTGGTACAGAGAGACACAAGGCTAAGAGACTCTACGTTTCATTGGCACCTCCGATGCAACAGGTGTGTGAGACCCATTGACGACGTGACCCTGGAGACCTCTCAGATCTTCGAGTTTCCGGATGTGTCGAAAAGAATATCCAGAATGGTTTCTGGGGCTGTGCCTCACTTCCAGAGGCTTCCCGATATCCGTCTGAGACCAGGAGATTTTGAATCTCTAAGCGGTAGAGAAAAGTCTCACCATATCGGATCAGCTCAGGGGCTCTTATACTCAATCTTAGTGGCAATTCACGACTCAGGATACAATGATGGAACCATCTTCCCTGTCAACATATACGGCAAGGTTTCCCCTAGAGACTATTTGAGAGGGCTCGCAAGGGGAGTATTGATAGGATCCTCGATTTGCTTCTTGACAAGAATGACAAATATCAATATTAATAGACCTCTTGAATTGGTCTCAGGGGTAATCTCATATATTCTCCTGAGGCTAGATAACCATCCCTCCTTGTACATAATGCTCAGAGAACCGTCTCTTAGAGGAGAGATATTTTCTATCCCTCAGAAAATCCCCGCCGCTTATCCAACCACTATGAAAGAAGGCAACAGATCAATCTTGTGTTATCTCCAACATGTGCTACGCTATGAGCGAGAGATAATCACGGCGTCTCCAGAGAATGACTGGCTATGGATCTTTTCAGACTTTAGAAGTGCCAAAATGACGTACCTATCCCTCATTACTTACCAGTCTCATCTTCTACTCCAGAGGGTTGAGAGAAACCTATCTAAGAGTATGAGAGATAACCTGCGACAATTGAGTTCTTTGATGAGGCAGGTGCTGGGCGGGCACGGAGAAGATACCTTAGAGTCAGACGACAACATTCAACGACTGCTAAAAGACTCTTTACGAAGGACAAGATGGGTGGATCAAGAGGTGCGCCATGCAGCTAGAACCATGACTGGAGATTACAGCCCCAACAAGAAGGTGTCCCGTAAGGTAGGATGTTCAGAATGGGTCTGCTCTGCTCAACAGGTTGCAGTCTCTACCTCAGCAAACCCGGCCCCTGTCTCGGAGCTTGACATAAGGGCCCTCTCTAAGAGGTTCCAGAACCCTTTGATCTCGGGCTTGAGAGTGGTTCAGTGGGCAACCGGTGCTCATTATAAGCTTAAGCCTATTCTAGATGATCTCAATGTTTTCCCATCTCTCTGCCTTGTAGTTGGGGACGGGTCAGGGGGGATATCAAGGGCAGTCCTCAACATGTTTCCAGATGCCAAGCTTGTGTTCAACAGTCTTTTAGAGGTGAATGACCTGATGGCTTCCGGAACACATCCACTGCCTCCTTCAGCAATCATGAGGGGAGGAAATGATATCGTCTCCAGAGTGATAGATCTTGACTCAATCTGGGAAAAACCGTCCGACTTGAGAAACTTGGCAACCTGGAAATACTTCCAGTCAGTCCAAAAGCAGGTCAACATGTCCTATGACCTCATTATTTGCGATGCAGAAGTTACTGACATTGCATCTATCAACCGGATCACCCTGTTAATGTCCGATTTTGCATTGTCTATAGATGGACCACTCTATTTGGTCTTCAAAACTTATGGGACTATGCTAGTAAATCCAAACTACAAGGCTATTCAACACCTGTCAAGAGCGTTCCCCTCGGTCACAGGGTTTATCACCCAAGTAACTTCGTCTTTTTCATCTGAGCTCTACCTCCGATTCTCCAAACGAGGGAAGTTTTTCAGAGATGCTGAGTACTTGACCTCTTCCACCCTTCGAGAAATGAGCCTTGTGTTATTCAATTGTAGCAGCCCCAAGAGTGAGATGCAGAGAGCTCGTTCCTTGAACTATCAGGATCTTGTGAGAGGATTTCCTGAAGAAATCATATCAAATCCTTACAATGAGATGATCATAACTCTGATTGACAGTGATGTAGAATCTTTTCTAGTCCACAAGATGGTTGATGATCTTGAGTTACAGAGGGGAACTCTGTCTAAAGTGGCTATCATTATAGCCATCATGATAGTTTTCTCCAACAGAGTCTTCAACGTTTCCAAACCCCTAACTGACCCCTCGTTCTATCCACCGTCTGATCCCAAAATCCTGAGGCACTTCAACATATGTTGCAGTACTATGATGTATCTATCTACTGCTTTAGGTGACGTCCCTAGCTTCGCAAGACTTCACGACCTGTATAACAGACCTATAACTTATTACTTCAGAAAGCAAGTCATTCGAGGGAACGTTTATCTATCTTGGAGTTGGTCCAACGACACCTCAGTGTTCAAAAGGGTAGCCTGTAATTCTAGCCTGAGTCTGTCATCTCACTGGATCAGGTTGATTTACAAGATAGTGAAGACTACCAGACTCGTTGGCAGCATCAAGGATCTATCCAGAGAAGTGGAAAGACACCTTCATAGGTACAACAGGTGGATCACCCTAGAGGATATCAGATCTAGATCATCCCTACTAGACTACAGTTGCCTGTGAACCGGATACTCCTGGAAGCCTGCCCATGCTAAGACTCTTGTGTGATGTATCTTGAAAAAAACAAGATCCTAAATCTGAACCTTTGGTTGTTTGATTGTTTTTCTCAtttttgttgtttatttgttaagcgt。
在其他实施方式中，核酸包括SEQ ID NO：9的序列，如下所示(RABV载体：CoravaxV2 South Africa(S1-RABVG-E51))：
ACGCTTAACAACCAGATCAAAGAAAAAACAGACATTGTCAATTGCAAAGCAAAAATGTAACACCCCTACAATGGATGCCGACAAGATTGTATTCAAAGTCAATAATCAGGTGGTCTCTTTGAAGCCTGAGATTATCGTGGATCAATATGAGTACAAGTACCCTGCCATCAAAGATTTGAAAAAGCCCTGTATAACCCTAGGAAAGGCTCCCGATTTAAATAAAGCATACAAGTCAGTTTTGTCAGGCATGAGCGCCGCCAAACTTAATCCTGACGATGTATGTTCCTATTTGGCAGCGGCAATGCAGTTTTTTGAGGGGACATGTCCGGAAGACTGGACCAGCTATGGAATTGTGATTGCACGAAAAGGAGATAAGATCACCCCAGGTTCTCTGGTGGAGATAAAACGTACTGATGTAGAAGGGAATTGGGCTCTGACAGGAGGCATGGAACTGACAAGAGACCCCACTGTCCCTGAGCATGCGTCCTTAGTCGGTCTTCTCTTGAGTCTGTATAGGTTGAGCAAAATATCCGGGCAAAACACTGGTAACTATAAGACAAACATTGCAGACAGGATAGAGCAGATTTTTGAGACAGCCCCTTTTGTTAAAATCGTGGAACACCATACTCTAATGACAACTCACAAaATGTGTGCTAATTGGAGTACTATACCAAACTTCAGATTTTTGGCCGGAACCTATGACATGTTTTTCTCCCGGATTGAGCATCTATATTCAGCAATCAGAGTGGGCACAGTTGTCACTGCTTATGAAGACTGTTCAGGACTGGTATCATTTACTGGGTTCATAAAACAAATCAATCTCACCGCTAGAGAGGCAATACTATATTTCTTCCACAAGAACTTTGAGGAAGAGATAAGAAGAATGTTTGAGCCAGGGCAGGAGACAGCTGTTCCTCACTCTTATTTCATCCACTTCCGTTCACTAGGCTTGAGTGGGAAATCTCCTTATTCATCAAATGCTGTTGGTCACGTGTTCAATCTCATTCACTTTGTAGGATGCTATATGGGTCAAGTCAGATCCCTAAATGCAACGGTTATTGCTGCATGTGCTCCTCATGAAATGTCTGTTCTAGGGGGCTATCTGGGAGAGGAATTCTTCGGGAAAGGGACATTTGAAAGAAGATTCTTCAGAGATGAGAAAGAACTTCAAGAATACGAGGCGGCTGAACTGACAAAGACTGACGTAGCACTGGCAGATGATGGAACTGTCAACTCTGACGACGAGGACTACTTTTCAGGTGAAACCAGAAGTCCGGAGGCTGTTTATACTCGAATCATGATGAATGGAGGTCGACTAAAGAGATCTCACATACGGAGATATGTCTCAGTCAGTTCCAATCATCAAGCCCGTCCAAACTCATTCGCCGAGTTTCTAAACAAGACATATTCGAGTGACTCATAAcatgaaaaaaactaacacccctcccgtacgCCACCATGTTCGTGTTTCTGGTGCTGCTGCCTCTGGTGAGCTCCCAGTGCGTGAACTTCACCACAAGGACCCAGCTGCCCCCTGCCTATACCAATTCCTTCACACGGGGCGTGTACTATCCCGACAAGGTGTTCCGGAGCAGCGTGCTGCACTCCACACAGGATCTGTTTCTGCCTTTCTTTTCTAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAATGGCACAAAGCGGTTCGCCAATCCAGTGCTGCCCTTTAACGATGGCGTGTACTTCGCCTCCACCGAGAAGTCTAACATCATCAGAGGCTGGATCTTTGGCACCACACTGGACAGCAAGACACAGTCCCTGCTGATCGTGAACAATGCCACCAACGTGGTCATCAAGGTGTGCGAGTTCCAGTTTTGTAATGATCCATTCCTGGGCGTGTACTATCACAAGAACAATAAGTCTTGGATGGAGAGCGAGTTTCGCGTGTATTCCTCTGCCAACAATTGCACATTTGAGTACGTGTCCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAATTTCAAGAACCTGAGGGAGTTCGTGTTTAAGAATATCGATGGCTACTTCAAAATCTACTCCAAGCACACCCCAATCAACCTGGTGCGCGGCCTGCCACAGGGCTTCTCTGCCCTGGAGCCACTGGTGGATCTGCCCATCGGCATCAACATCACCCGGTTTCAGACACTGCTGGCCCTGCACAGAAGCTACCTGACACCAGGCGACAGCTCCTCTGGATGGACCGCAGGAGCAGCAGCCTACTATGTGGGCTATCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAATGGCACCATCACAGACGCCGTGGATTGCGCCCTGGATCCCCTGTCTGAGACCAAGTGTACACTGAAGAGCTTTACCGTGGAGAAGGGCATCTATCAGACAAGCAATTTCAGGGTGCAGCCTACCGAGTCCATCGTGCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAATATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGAAGGGCTTTAACTGTTATTTCCCTCTGCAGAGCTACGGCTTCCAGCCAACATATGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACCTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGACTGACCGGAACAGGCGTGCTGACCGAGTCCAACAAGAAGTTCCTGCCTTTTCAGCAGTTCGGCAGGGACATCGCAGATACCACAGACGCCGTGCGCGACCCTCAGACCCTGGAGATCCTGGACATCACACCATGCTCCTTCGGCGGCGTGTCTGTGATCACACCAGGCACCAATACAAGCAACCAGGTGGCCGTGCTGTATCAGGGCGTGAATTGTACCGAGGTGCCAGTGGCAATCCACGCAGATCAGCTGACCCCTACATGGCGGGTGTACTCTACCGGCAGCAACGTGTTCCAGACAAGAGCCGGATGCCTGATCGGAGCAGAGCACGTGAACAATAGCTATGAGTGCGACATCCCTATCGGCGCCGGCATCTGTGCCTCCTACCAGACCCAGACAAACTCCCCAGAATCAAGCGTGATTCCTCTGGTCCATCCACTGGCAGATCCCTCCACAGTGTTCAAAGACGGAGATGAGGCCGAAGACTTTGTGGAAGTCCACCTGCCTGATGTGCATAACCAGGTGTCTGGCGTCGACCTGGGACTGCCAAATTGGGGCAAGTACGTGCTGCTGAGTGCTGGAGCACTGACTGCCCTGATGCTGATCATTTTCCTGATGACCTGCTGTCGGCGCGTGAACAGAAGTGAGCCCACTCAGCACAATCTGCGAGGAACCGGGAGAGAAGTGTCAGTCACACCTCAGAGCGGGAAAATCATTAGTAGTTGGGAATCACATAAAAGCGGGGGCGAGACCAGGCTGTGAgctagcCATGAAAAAAACTAACACCCCTCCTTTCGAACCATCCCAAACATGAGCAAGATCTTTGTCAATCCTAGTGCTATTAGAGCCGGTCTGGCCGATCTTGAGATGGCTGAAGAAACTGTTGATCTGATCAATAGAAATATCGAAGACAATCAGGCTCATCTCCAAGGGGAACCCATAGAGGTGGACAATCTCCCTGAGGATATGGGGCGACTTCACCTGGATGATGGAAAATCGCCCAACCATGGTGAGATAGCCAAGGTGGGAGAAGGCAAGTATCGAGAGGACTTTCAGATGGATGAAGGAGAGGATCCTAGCTTCCTGTTCCAGTCATACCTGGAAAATGTTGGAGTCCAAATAGTCAGACAAATGAGGTCAGGAGAGAGATTTCTCAAGATATGGTCACAGACCGTAGAAGAGATTATATCCTATGTCGCGGTCAACTTTCCCAACCCTCCAGGAAAGTCTTCAGAGGATAAATCAACCCAGACTACTGGCCGAGAGCTCAAGAAGGAGACAACACCCACTCCTTCTCAGAGAGAAAGCCAATCATCGAAAGCCAGGATGGCGGCTCAAATTGCTTCTGGCCCTCCAGCCCTTGAATGGTCGGCTACCAATGAAGAGGATGATCTATCAGTGGAGGCTGAGATCGCTCACCAGATTGCAGAAAGTTTCTCCAAAAAATATAAGTTTCCCTCTCGATCCTCAGGGATACTCTTGTATAATTTTGAGCAATTGAAAATGAACCTTGATGATATAGTTAAAGAGGCAAAAAATGTACCAGGTGTGACCCGTTTAGCCCATGACGGGTCCAAACTCCCCCTAAGATGTGTACTGGGATGGGTCGCTTTGGCCAACTCTAAGAAATTCCAGTTGTTAGTCGAATCCGACAAGCTGAGTAAAATCATGCAAGATGACTTGAATCGCTATACATCTTGCTAACCGAACCTCTCCCCTCAGTCCCTCTAGACAATAAAATCCGAGATGTCCCAAAGTCAACATGAAAAAAACAGGCAACACCACTGATAAAATGAACCTCCTACGTAAGATAGTGAAAAACCGCAGGGACGAGGACACTCAAAAATCCTCTCCCGCGTCAGCCCCTCTGGATGACGATGACTTGTGGCTTCCACCCCCTGAATACGTCCCGCTGAAAGAACTTACAGGCAAGAAGAACATGAGGAACTTTTGTATCAACGGAAGGGTTAAAGTGTGTAGCCCGAATGGTTACTCGTTCAGGATCCTGCGGCACATTCTGAAATCATTCGACGAGATATATTCTGGGAATCATAGGATGATCGGGTTAGTCAAAGTGGTTATTGGACTGGCTTTGTCAGGATCTCCAGTCCCTGAGGGCCTGAACTGGGTATACAAATTGAGGAGAACCTTTATCTTCCAGTGGGCTGATTCCAGGGGCCCTCTTGAAGGGGAGGAGTTGGAATACTCTCAGGAGATCACTTGGGATGATGATACTGAGTTCGTCGGATTGCAAATAAGAGTGATTGCAAAACAGTGTCATATCCAGGGCAGAGTCTGGTGTATCAACATGAACCCGAGAGCATGTCAACTATGGTCTGACATGTCTCTTCAGACACAAAGGTCCGAAGAGGACAAAGATTCCTCTCTGCTTCTAGAATAATCAGATTATATCCCGCAAATTTATCACTTGTTTACCTCTGGAGGAGAGAACATATGGGCTCAACTCCAACCCTTGGGAGCAATATAACAAAAAACATGTTATGGTGCCATTAAACCGCTGCATTTCATCAAAGTCAAGTTGATTACCTTTACATTTTGATCCTCTTGGATGTGAAAAAAACTATTAACATCCCTCAAAAGACcccgggAAAGATGGTTCCTCAGGCTCTCCTGTTTGTACCCCTTCTGGTTTTTCCATTGTGTTTTGGGAAATTCCCTATTTACACGATACCAGACAAGCTTGGTCCCTGGAGTCCGATTGACATACATCACCTCAGCTGCCCAAACAATTTGGTAGTGGAGGACGAAGGATGCACCAACCTGTCAGGGTTCTCCTACATGGAACTTAAAGTTGGATACATCTTAGCCATAAAAGTGAACGGGTTCACTTGCACAGGCGTTGTGACGGAGGCTGAAACCTACACTAACTTCGTTGGTTATGTCACAACCACGTTCAAAAGAAAGCATTTCCGCCCAACACCAGATGCATGTAGAGCCGCGTACAACTGGAAGATGGCCGGTGACCCCAGATATGAAGAGTCTCTACACAATCCGTACCCTGACTACCGCTGGCTTCGAACTGTAAAAACCACCAAGGAGTCTCTCGTTATCATATCTCCAAGTGTGGCAGATTTGGACCCATATGACAGATCCCTTCACTCGAGGGTCTTCCCTAGCGGGAAGTGCTCAGGAGTAGCGGTGTCTTCTACCTACTGCTCCACTAACCACGATTACACCATTTGGATGCCCGAGAATCCGAGACTAGGGATGTCTTGTGACATTTTTACCAATAGTAGAGGGAAGAGAGCATCCAAAGGGAGTGAGACTTGCGGCTTTGTAGATGAAAGAGGCCTATATAAGTCTTTAAAAGGAGCATGCAAACTCAAGTTATGTGGAGTTCTAGGACTTAGACTTATGGATGGAACATGGGTCTCGATGCAAACATCAAATGAAACCAAATGGTGCCCTCCCGATAAGTTGGTGAACCTGCACGACTTTCGCTCAGACGAAATTGAGCACCTTGTTGTAGAGGAGTTGGTCAGGAAGAGAGAGGAGTGTCTGGATGCACTAGAGTCCATCATGACAACCAAGTCAGTGAGTTTCAGACGTCTCAGTCATTTAAGAAAACTTGTCCCTGGGTTTGGAAAAGCATATACCATATTCAACAAGACCTTGATGGAAGCCGATGCTCACTACAAGTCAGTCgagACTTGGAATGAGATCCTCCCTTCAAAAGGGTGTTTAAGAGTTGGGGGGAGGTGTCATCCTCATGTGAACGGGGTGTTTTTCAATGGTATAATATTAGGACCTGACGGCAATGTCTTAATCCCAGAGATGCAATCATCCCTCCTCCAGCAACATATGGAGTTGTTGGAATCCTCGGTTATCCCCCTTGTGCACCCCCTGGCAGACCCGTCTACCGTTTTCAAGGACGGTGACGAGGCTGAGGATTTTGTTGAAGTTCACCTTCCCGATGTGCACAATCAGGTCTCAGGAGTTGACTTGGGTCTCCCGAACTGGGGGAAGTATGTATTACTGAGTGCAGGGGCCCTGACTGCCTTGATGTTGATAATTTTCCTGATGACATGTTGTAGAAGAGTCAATCGATCAGAACCTACGCAACACAATCTCAGAGGGACAGGGAGGGAGGTGTCAGTCACTCCCCAAAGCGGGAAGATCATATCTTCATGGGAATCACACAAGAGTGGGGGTGAGACCAGACTGTAAttaattaaCGTCCTTTCAACGATCCAAGTCcatgaaaaaaactaacacccctcccgtacctagcTTATAAAGTGCTGGGTCATCTAAGCTTTTCAGTCGAGAAAAAAACATTAGATCAGAAGAACAACTGGCAACACTTCTCAACCTGAGACTTACTTCAAGATGCTCGATCCTGGAGAGGTCTATGATGACCCTATTGACCCAATCGAGTTAGAGGCTGAACCCAGAGGAACCCCCATTGTCCCCAACATCTTGAGGAACTCTGACTACAATCTCAACTCTCCTTTGATAGAAGATCCTGCTAGACTAATGTTAGAATGGTTAAAAACAGGGAATAGACCTTATCGGATGACTCTAACAGACAATTGCTCCAGGTCTTTCAGAGTTTTGAAAGATTATTTCAAGAAGGTAGATTTGGGTTCTCTCAAGGTGGGCGGAATGGCTGCACAGTCAATGATTTCTCTCTGGTTATATGGTGCCCACTCTGAATCCAACAGGAGCCGGAGATGTATAACAGACTTGGCCCATTTCTATTCCAAGTCGTCCCCCATAGAGAAGCTGTTGAATCTCACGCTAGGAAATAGAGGGCTGAGAATCCCCCCAGAGGGAGTGTTAAGTTGCCTTGAGAGGGTTGATTATGATAATGCATTTGGAAGGTATCTTGCCAACACGTATTCCTCTTACTTGTTCTTCCATGTAATCACCTTATACATGAACGCCCTAGACTGGGATGAAGAAAAGACCATCCTAGCATTATGGAAAGATTTAACCTCAGTGGACATCGGGAAGGACTTGGTAAAGTTCAAAGACCAAATATGGGGACTGCTGATCGTGACAAAGGACTTTGTTTACTCCCAAAGTTCCAATTGTCTTTTTGACAGAAACTACACACTTATGCTAAAAGATCTTTTCTTGTCTCGCTTCAACTCCTTAATGGTCTTGCTCTCTCCCCCAGAGCCCCGATACTCAGATGACTTGATATCTCAACTATGCCAGCTGTACATTGCTGGGGATCAAGTCTTGTCTATGTGTGGAAACTCCGGCTATGAAGTCATCAAAATATTGGAGCCATATGTCGTGAATAGTTTAGTCCAGAGAGCAGAAAAGTTTAGGCCTCTCATTCATTCCTTGGGAGACTTTCCTGTATTTATAAAAGACAAGGTAAGTCAACTTGAAGAGACGTTCGGTCCCTGTGCAAGAAGGTTCTTTAGGGCTCTGGATCAATTCGACAACATACATGACTTGGTTTTTGTGTTTGGCTGTTACAGGCATTGGGGGCACCCATATATAGATTATCGAAAGGGTCTGTCAAAACTATATGATCAGGTTCACCTTAAAAAAATGATAGATAAGTCCTACCAGGAGTGCTTAGCAAGCGACCTAGCCAGGAGGATCCTTAGATGGGGTTTTGATAAGTACTCCAAGTGGTATCTGGATTCAAGATTCCTAGCCCGAGACCACCCCTTGACTCCTTATATCAAAACCCAAACATGGCCACCCAAACATATTGTAGACTTGGTGGGGGATACATGGCACAAGCTCCCGATCACGCAGATCTTTGAGATTCCTGAATCAATGGATCCGTCAGAAATATTGGATGACAAATCACATTCTTTCACCAGAACGAGACTAGCTTCTTGGCTGTCAGAAAACCGAGGGGGGCCTGTTCCTAGCGAAAAAGTTATTATCACGGCCCTGTCTAAGCCGCCTGTCAATCCCCGAGAGTTTCTGAGGTCTATAGACCTCGGAGGATTGCCAGATGAAGACTTGATAATTGGCCTCAAGCCAAAGGAACGGGAATTGAAGATTGAAGGTCGATTCTTTGCTCTAATGTCATGGAATCTAAGATTGTATTTTGTCATCACTGAAAAACTCTTGGCCAACTACATCTTGCCACTTTTTGACGCGCTGACTATGACAGACAACCTGAACAAGGTGTTTAAAAAGCTGATCGACAGGGTCACCGGGCAAGGGCTTTTGGACTATTCAAGGGTCACATATGCATTTCACCTGGACTATGAAAAGTGGAACAACCATCAAAGATTAGAGTCAACAGAGGATGTATTTTCTGTCCTAGATCAAGTGTTTGGATTGAAGAGAGTGTTTTCTAGAACACACGAGTTTTTTCAAAAGGCCTGGATCTATTATTCAGACAGATCAGACCTCATCGGGTTACGGGAGGATCAAATATACTGCTTAGATGCGTCCAACGGCCCAACCTGTTGGAATGGCCAGGATGGCGGGCTAGAAGGCTTACGGCAGAAGGGCTGGAGTCTAGTCAGCTTATTGATGATAGATAGAGAATCTCAAATCAGGAACACAAGAACCAAAATACTAGCTCAAGGAGACAACCAGGTTTTATGTCCGACATACATGTTGTCGCCAGGGCTATCTCAAGAGGGGCTCCTCTATGAATTGGAGAGAATATCAAGGAATGCACTTTCGATATACAGAGCCGTCGAGGAAGGGGCATCTAAGCTAGGGCTGATCATCAAGAAAGAAGAGACCATGTGTAGTTATGACTTCCTCATCTATGGAAAAACCCCTTTGTTTAGAGGTAACATATTGGTGCCTGAGTCCAAAAGATGGGCCAGAGTCTCTTGCGTCTCTAATGACCAAATAGTCAACCTCGCCAATATAATGTCGACAGTGTCCACCAATGCGCTAACAGTGGCACAACACTCTCAATCTTTGATCAAACCGATGAGGGATTTTCTGCTCATGTCAGTACAGGCAGTCTTTCACTACCTGCTATTTAGCCCAATCTTAAAGGGAAGAGTTTACAAGATTCTGAGCGCTGAAGGGGAGAGCTTTCTCCTAGCCATGTCAAGGATAATCTATCTAGATCCTTCTTTGGGAGGGATATCTGGAATGTCCCTCGGAAGATTCCATATACGACAGTTCTCAGACCCTGTCTCTGAAGGGTTATCCTTCTGGAGAGAGATCTGGTTAAGCTCCCAAGAGTCCTGGATTCACGCGTTGTGTCAAGAGGCTGGAAACCCAGATCTTGGAGAGAGAACACTCGAGAGCTTCACTCGCCTTCTAGAAGATCCGACCACCTTAAATATCAGAGGAGGGGCCAGTCCTACCATTCTACTCAAGGATGCAATCAGAAAGGCTTTATATGACGAGGTGGACAAGGTGGAAAATTCAGAGTTTCGAGAGGCAATCCTGTTGTCCAAGACCCATAGAGATAATTTTATACTCTTCTTAATATCTGTTGAGCCTCTGTTTCCTCGATTTCTCAGTGAGCTATTCAGTTCGTCTTTTTTGGGAATCCCCGAGTCAATCATTGGATTGATACAAAACTCCCGAACGATAAGAAGGCAGTTTAGAAAGAGTCTCTCAAAAACTTTAGAAGAATCCTTCTACAACTCAGAGATCCACGGGATTAGTCGGATGACCCAGACACCTCAGAGGGTTGGGGGGGTGTGGCCTTGCTCTTCAGAGAGGGCAGATCTACTTAGGGAGATCTCTTGGGGAAGAAAAGTGGTAGGCACGACAGTTCCTCACCCTTCTGAGATGTTGGGATTACTTCCCAAGTCCTCTATTTCTTGCACTTGTGGAGCAACAGGAGGAGGCAATCCTAGAGTTTCTGTATCAGTACTCCCGTCCTTTGATCAGTCATTTTTTTCACGAGGCCCCCTAAAGGGATACTTGGGCTCGTCCACCTCTATGTCGACCCAGCTATTCCATGCATGGGAAAAAGTCACTAATGTTCATGTGGTGAAGAGAGCTCTATCGTTAAAAGAATCTATAAACTGGTTCATTACTAGAGATTCCAACTTGGCTCAAGCTCTAATTAGGAACATTATGTCTCTGACAGGCCCTGATTTCCCTCTAGAGGAGGCCCCTGTCTTCAAAAGGACGGGGTCAGCCTTGCATAGGTTCAAGTCTGCCAGATACAGCGAAGGAGGGTATTCTTCTGTCTGCCCGAACCTCCTCTCTCATATTTCTGTTAGTACAGACACCATGTCTGATTTGACCCAAGACGGGAAGAACTACGATTTCATGTTCCAGCCATTGATGCTTTATGCACAGACATGGACATCAGAGCTGGTACAGAGAGACACAAGGCTAAGAGACTCTACGTTTCATTGGCACCTCCGATGCAACAGGTGTGTGAGACCCATTGACGACGTGACCCTGGAGACCTCTCAGATCTTCGAGTTTCCGGATGTGTCGAAAAGAATATCCAGAATGGTTTCTGGGGCTGTGCCTCACTTCCAGAGGCTTCCCGATATCCGTCTGAGACCAGGAGATTTTGAATCTCTAAGCGGTAGAGAAAAGTCTCACCATATCGGATCAGCTCAGGGGCTCTTATACTCAATCTTAGTGGCAATTCACGACTCAGGATACAATGATGGAACCATCTTCCCTGTCAACATATACGGCAAGGTTTCCCCTAGAGACTATTTGAGAGGGCTCGCAAGGGGAGTATTGATAGGATCCTCGATTTGCTTCTTGACAAGAATGACAAATATCAATATTAATAGACCTCTTGAATTGGTCTCAGGGGTAATCTCATATATTCTCCTGAGGCTAGATAACCATCCCTCCTTGTACATAATGCTCAGAGAACCGTCTCTTAGAGGAGAGATATTTTCTATCCCTCAGAAAATCCCCGCCGCTTATCCAACCACTATGAAAGAAGGCAACAGATCAATCTTGTGTTATCTCCAACATGTGCTACGCTATGAGCGAGAGATAATCACGGCGTCTCCAGAGAATGACTGGCTATGGATCTTTTCAGACTTTAGAAGTGCCAAAATGACGTACCTATCCCTCATTACTTACCAGTCTCATCTTCTACTCCAGAGGGTTGAGAGAAACCTATCTAAGAGTATGAGAGATAACCTGCGACAATTGAGTTCTTTGATGAGGCAGGTGCTGGGCGGGCACGGAGAAGATACCTTAGAGTCAGACGACAACATTCAACGACTGCTAAAAGACTCTTTACGAAGGACAAGATGGGTGGATCAAGAGGTGCGCCATGCAGCTAGAACCATGACTGGAGATTACAGCCCCAACAAGAAGGTGTCCCGTAAGGTAGGATGTTCAGAATGGGTCTGCTCTGCTCAACAGGTTGCAGTCTCTACCTCAGCAAACCCGGCCCCTGTCTCGGAGCTTGACATAAGGGCCCTCTCTAAGAGGTTCCAGAACCCTTTGATCTCGGGCTTGAGAGTGGTTCAGTGGGCAACCGGTGCTCATTATAAGCTTAAGCCTATTCTAGATGATCTCAATGTTTTCCCATCTCTCTGCCTTGTAGTTGGGGACGGGTCAGGGGGGATATCAAGGGCAGTCCTCAACATGTTTCCAGATGCCAAGCTTGTGTTCAACAGTCTTTTAGAGGTGAATGACCTGATGGCTTCCGGAACACATCCACTGCCTCCTTCAGCAATCATGAGGGGAGGAAATGATATCGTCTCCAGAGTGATAGATCTTGACTCAATCTGGGAAAAACCGTCCGACTTGAGAAACTTGGCAACCTGGAAATACTTCCAGTCAGTCCAAAAGCAGGTCAACATGTCCTATGACCTCATTATTTGCGATGCAGAAGTTACTGACATTGCATCTATCAACCGGATCACCCTGTTAATGTCCGATTTTGCATTGTCTATAGATGGACCACTCTATTTGGTCTTCAAAACTTATGGGACTATGCTAGTAAATCCAAACTACAAGGCTATTCAACACCTGTCAAGAGCGTTCCCCTCGGTCACAGGGTTTATCACCCAAGTAACTTCGTCTTTTTCATCTGAGCTCTACCTCCGATTCTCCAAACGAGGGAAGTTTTTCAGAGATGCTGAGTACTTGACCTCTTCCACCCTTCGAGAAATGAGCCTTGTGTTATTCAATTGTAGCAGCCCCAAGAGTGAGATGCAGAGAGCTCGTTCCTTGAACTATCAGGATCTTGTGAGAGGATTTCCTGAAGAAATCATATCAAATCCTTACAATGAGATGATCATAACTCTGATTGACAGTGATGTAGAATCTTTTCTAGTCCACAAGATGGTTGATGATCTTGAGTTACAGAGGGGAACTCTGTCTAAAGTGGCTATCATTATAGCCATCATGATAGTTTTCTCCAACAGAGTCTTCAACGTTTCCAAACCCCTAACTGACCCCTCGTTCTATCCACCGTCTGATCCCAAAATCCTGAGGCACTTCAACATATGTTGCAGTACTATGATGTATCTATCTACTGCTTTAGGTGACGTCCCTAGCTTCGCAAGACTTCACGACCTGTATAACAGACCTATAACTTATTACTTCAGAAAGCAAGTCATTCGAGGGAACGTTTATCTATCTTGGAGTTGGTCCAACGACACCTCAGTGTTCAAAAGGGTAGCCTGTAATTCTAGCCTGAGTCTGTCATCTCACTGGATCAGGTTGATTTACAAGATAGTGAAGACTACCAGACTCGTTGGCAGCATCAAGGATCTATCCAGAGAAGTGGAAAGACACCTTCATAGGTACAACAGGTGGATCACCCTAGAGGATATCAGATCTAGATCATCCCTACTAGACTACAGTTGCCTGTGAACCGGATACTCCTGGAAGCCTGCCCATGCTAAGACTCTTGTGTGATGTATCTTGAAAAAAACAAGATCCTAAATCTGAACCTTTGGTTGTTTGATTGTTTTTCTCAtttttgttgtttatttgttaagcgt。
在一个实施方式中，核酸包括SEQ ID NO：10的序列，如下所示(RABV载体：CoravaxV3-China(S1-VSVG-E26))：
ACGCTTAACAACCAGATCAAAGAAAAAACAGACATTGTCAATTGCAAAGCAAAAATGTAACACCCCTACAATGGATGCCGACAAGATTGTATTCAAAGTCAATAATCAGGTGGTCTCTTTGAAGCCTGAGATTATCGTGGATCAATATGAGTACAAGTACCCTGCCATCAAAGATTTGAAAAAGCCCTGTATAACCCTAGGAAAGGCTCCCGATTTAAATAAAGCATACAAGTCAGTTTTGTCAGGCATGAGCGCCGCCAAACTTAATCCTGACGATGTATGTTCCTATTTGGCAGCGGCAATGCAGTTTTTTGAGGGGACATGTCCGGAAGACTGGACCAGCTATGGAATTGTGATTGCACGAAAAGGAGATAAGATCACCCCAGGTTCTCTGGTGGAGATAAAACGTACTGATGTAGAAGGGAATTGGGCTCTGACAGGAGGCATGGAACTGACAAGAGACCCCACTGTCCCTGAGCATGCGTCCTTAGTCGGTCTTCTCTTGAGTCTGTATAGGTTGAGCAAAATATCCGGGCAAAACACTGGTAACTATAAGACAAACATTGCAGACAGGATAGAGCAGATTTTTGAGACAGCCCCTTTTGTTAAAATCGTGGAACACCATACTCTAATGACAACTCACAAaATGTGTGCTAATTGGAGTACTATACCAAACTTCAGATTTTTGGCCGGAACCTATGACATGTTTTTCTCCCGGATTGAGCATCTATATTCAGCAATCAGAGTGGGCACAGTTGTCACTGCTTATGAAGACTGTTCAGGACTGGTATCATTTACTGGGTTCATAAAACAAATCAATCTCACCGCTAGAGAGGCAATACTATATTTCTTCCACAAGAACTTTGAGGAAGAGATAAGAAGAATGTTTGAGCCAGGGCAGGAGACAGCTGTTCCTCACTCTTATTTCATCCACTTCCGTTCACTAGGCTTGAGTGGGAAATCTCCTTATTCATCAAATGCTGTTGGTCACGTGTTCAATCTCATTCACTTTGTAGGATGCTATATGGGTCAAGTCAGATCCCTAAATGCAACGGTTATTGCTGCATGTGCTCCTCATGAAATGTCTGTTCTAGGGGGCTATCTGGGAGAGGAATTCTTCGGGAAAGGGACATTTGAAAGAAGATTCTTCAGAGATGAGAAAGAACTTCAAGAATACGAGGCGGCTGAACTGACAAAGACTGACGTAGCACTGGCAGATGATGGAACTGTCAACTCTGACGACGAGGACTACTTTTCAGGTGAAACCAGAAGTCCGGAGGCTGTTTATACTCGAATCATGATGAATGGAGGTCGACTAAAGAGATCTCACATACGGAGATATGTCTCAGTCAGTTCCAATCATCAAGCCCGTCCAAACTCATTCGCCGAGTTTCTAAACAAGACATATTCGAGTGACTCATAAcatgaaaaaaactaacacccctcccGTACGCCACCATGTTCGTGTTTCTGGTGCTGCTGCCTCTGGTGAGCTCCCAGTGCGTGAACCTGACCACAAGGACCCAGCTGCCCCCTGCCTATACCAATTCCTTCACACGGGGCGTGTACTATCCCGACAAGGTGTTCCGGAGCAGCGTGCTGCACTCCACACAGGATCTGTTTCTGCCTTTCTTTTCTAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAATGGCACAAAGCGGTTCGACAATCCAGTGCTGCCCTTTAACGATGGCGTGTACTTCGCCTCCACCGAGAAGTCTAACATCATCAGAGGCTGGATCTTTGGCACCACACTGGACAGCAAGACACAGTCCCTGCTGATCGTGAACAATGCCACCAACGTGGTCATCAAGGTGTGCGAGTTCCAGTTTTGTAATGATCCATTCCTGGGCGTGTACTATCACAAGAACAATAAGTCTTGGATGGAGAGCGAGTTTCGCGTGTATTCCTCTGCCAACAATTGCACATTTGAGTACGTGTCCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAATTTCAAGAACCTGAGGGAGTTCGTGTTTAAGAATATCGATGGCTACTTCAAAATCTACTCCAAGCACACCCCAATCAACCTGGTGCGCGACCTGCCACAGGGCTTCTCTGCCCTGGAGCCACTGGTGGATCTGCCCATCGGCATCAACATCACCCGGTTTCAGACACTGCTGGCCCTGCACAGAAGCTACCTGACACCAGGCGACAGCTCCTCTGGATGGACCGCAGGAGCAGCAGCCTACTATGTGGGCTATCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAATGGCACCATCACAGACGCCGTGGATTGCGCCCTGGATCCCCTGTCTGAGACCAAGTGTACACTGAAGAGCTTTACCGTGGAGAAGGGCATCTATCAGACAAGCAATTTCAGGGTGCAGCCTACCGAGTCCATCGTGCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAAGATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGGAGGGCTTTAACTGTTATTTCCCTCTGCAGAGCTACGGCTTCCAGCCAACAAACGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACCTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGACTGACCGGAACAGGCGTGCTGACCGAGTCCAACAAGAAGTTCCTGCCTTTTCAGCAGTTCGGCAGGGACATCGCAGATACCACAGACGCCGTGCGCGACCCTCAGACCCTGGAGATCCTGGACATCACACCATGCTCCTTCGGCGGCGTGTCTGTGATCACACCAGGCACCAATACAAGCAACCAGGTGGCCGTGCTGTATCAGGACGTGAATTGTACCGAGGTGCCAGTGGCAATCCACGCAGATCAGCTGACCCCTACATGGCGGGTGTACTCTACCGGCAGCAACGTGTTCCAGACAAGAGCCGGATGCCTGATCGGAGCAGAGCACGTGAACAATAGCTATGAGTGCGACATCCCTATCGGCGCCGGCATCTGTGCCTCCTACCAGACCCAGACAAACTCCCCAAGGTCTGTGGGCGATACAGGCCTGTCCAAGAATCCAATCGAGCTGGTAGAGGGCTGGTTCAGCAGTTGGAAAAGCTCCATCGCCTCCTTTTTCTTTATCATCGGCCTGATCATCGGACTGTTCCTGGTGCTCCGCGTGGGTATCCACCTGTGCATCAAGCTGAAGCACACCAAGAAAAGACAGATTTATACAGACATCGAGATGAACCGCCTGGGAAAGTGAGCTAGCCATGAAAAAAACTAACACCCCTCCTTTCGAACCATCCCAAACATGAGCAAGATCTTTGTCAATCCTAGTGCTATTAGAGCCGGTCTGGCCGATCTTGAGATGGCTGAAGAAACTGTTGATCTGATCAATAGAAATATCGAAGACAATCAGGCTCATCTCCAAGGGGAACCCATAGAGGTGGACAATCTCCCTGAGGATATGGGGCGACTTCACCTGGATGATGGAAAATCGCCCAACCATGGTGAGATAGCCAAGGTGGGAGAAGGCAAGTATCGAGAGGACTTTCAGATGGATGAAGGAGAGGATCCTAGCTTCCTGTTCCAGTCATACCTGGAAAATGTTGGAGTCCAAATAGTCAGACAAATGAGGTCAGGAGAGAGATTTCTCAAGATATGGTCACAGACCGTAGAAGAGATTATATCCTATGTCGCGGTCAACTTTCCCAACCCTCCAGGAAAGTCTTCAGAGGATAAATCAACCCAGACTACTGGCCGAGAGCTCAAGAAGGAGACAACACCCACTCCTTCTCAGAGAGAAAGCCAATCATCGAAAGCCAGGATGGCGGCTCAAATTGCTTCTGGCCCTCCAGCCCTTGAATGGTCGGCTACCAATGAAGAGGATGATCTATCAGTGGAGGCTGAGATCGCTCACCAGATTGCAGAAAGTTTCTCCAAAAAATATAAGTTTCCCTCTCGATCCTCAGGGATACTCTTGTATAATTTTGAGCAATTGAAAATGAACCTTGATGATATAGTTAAAGAGGCAAAAAATGTACCAGGTGTGACCCGTTTAGCCCATGACGGGTCCAAACTCCCCCTAAGATGTGTACTGGGATGGGTCGCTTTGGCCAACTCTAAGAAATTCCAGTTGTTAGTCGAATCCGACAAGCTGAGTAAAATCATGCAAGATGACTTGAATCGCTATACATCTTGCTAACCGAACCTCTCCCCTCAGTCCCTCTAGACAATAAAATCCGAGATGTCCCAAAGTCAACATGAAAAAAACAGGCAACACCACTGATAAAATGAACCTCCTACGTAAGATAGTGAAAAACCGCAGGGACGAGGACACTCAAAAATCCTCTCCCGCGTCAGCCCCTCTGGATGACGATGACTTGTGGCTTCCACCCCCTGAATACGTCCCGCTGAAAGAACTTACAGGCAAGAAGAACATGAGGAACTTTTGTATCAACGGAAGGGTTAAAGTGTGTAGCCCGAATGGTTACTCGTTCAGGATCCTGCGGCACATTCTGAAATCATTCGACGAGATATATTCTGGGAATCATAGGATGATCGGGTTAGTCAAAGTGGTTATTGGACTGGCTTTGTCAGGATCTCCAGTCCCTGAGGGCCTGAACTGGGTATACAAATTGAGGAGAACCTTTATCTTCCAGTGGGCTGATTCCAGGGGCCCTCTTGAAGGGGAGGAGTTGGAATACTCTCAGGAGATCACTTGGGATGATGATACTGAGTTCGTCGGATTGCAAATAAGAGTGATTGCAAAACAGTGTCATATCCAGGGCAGAGTCTGGTGTATCAACATGAACCCGAGAGCATGTCAACTATGGTCTGACATGTCTCTTCAGACACAAAGGTCCGAAGAGGACAAAGATTCCTCTCTGCTTCTAGAATAATCAGATTATATCCCGCAAATTTATCACTTGTTTACCTCTGGAGGAGAGAACATATGGGCTCAACTCCAACCCTTGGGAGCAATATAACAAAAAACATGTTATGGTGCCATTAAACCGCTGCATTTCATCAAAGTCAAGTTGATTACCTTTACATTTTGATCCTCTTGGATGTGAAAAAAACTATTAACATCCCTCAAAAGACcccgggAAAGATGGTTCCTCAGGCTCTCCTGTTTGTACCCCTTCTGGTTTTTCCATTGTGTTTTGGGAAATTCCCTATTTACACGATACCAGACAAGCTTGGTCCCTGGAGTCCGATTGACATACATCACCTCAGCTGCCCAAACAATTTGGTAGTGGAGGACGAAGGATGCACCAACCTGTCAGGGTTCTCCTACATGGAACTTAAAGTTGGATACATCTTAGCCATAAAAGTGAACGGGTTCACTTGCACAGGCGTTGTGACGGAGGCTGAAACCTACACTAACTTCGTTGGTTATGTCACAACCACGTTCAAAAGAAAGCATTTCCGCCCAACACCAGATGCATGTAGAGCCGCGTACAACTGGAAGATGGCCGGTGACCCCAGATATGAAGAGTCTCTACACAATCCGTACCCTGACTACCGCTGGCTTCGAACTGTAAAAACCACCAAGGAGTCTCTCGTTATCATATCTCCAAGTGTGGCAGATTTGGACCCATATGACAGATCCCTTCACTCGAGGGTCTTCCCTAGCGGGAAGTGCTCAGGAGTAGCGGTGTCTTCTACCTACTGCTCCACTAACCACGATTACACCATTTGGATGCCCGAGAATCCGAGACTAGGGATGTCTTGTGACATTTTTACCAATAGTAGAGGGAAGAGAGCATCCAAAGGGAGTGAGACTTGCGGCTTTGTAGATGAAAGAGGCCTATATAAGTCTTTAAAAGGAGCATGCAAACTCAAGTTATGTGGAGTTCTAGGACTTAGACTTATGGATGGAACATGGGTCTCGATGCAAACATCAAATGAAACCAAATGGTGCCCTCCCGATAAGTTGGTGAACCTGCACGACTTTCGCTCAGACGAAATTGAGCACCTTGTTGTAGAGGAGTTGGTCAGGAAGAGAGAGGAGTGTCTGGATGCACTAGAGTCCATCATGACAACCAAGTCAGTGAGTTTCAGACGTCTCAGTCATTTAAGAAAACTTGTCCCTGGGTTTGGAAAAGCATATACCATATTCAACAAGACCTTGATGGAAGCCGATGCTCACTACAAGTCAGTCgagACTTGGAATGAGATCCTCCCTTCAAAAGGGTGTTTAAGAGTTGGGGGGAGGTGTCATCCTCATGTGAACGGGGTGTTTTTCAATGGTATAATATTAGGACCTGACGGCAATGTCTTAATCCCAGAGATGCAATCATCCCTCCTCCAGCAACATATGGAGTTGTTGGAATCCTCGGTTATCCCCCTTGTGCACCCCCTGGCAGACCCGTCTACCGTTTTCAAGGACGGTGACGAGGCTGAGGATTTTGTTGAAGTTCACCTTCCCGATGTGCACAATCAGGTCTCAGGAGTTGACTTGGGTCTCCCGAACTGGGGGAAGTATGTATTACTGAGTGCAGGGGCCCTGACTGCCTTGATGTTGATAATTTTCCTGATGACATGTTGTAGAAGAGTCAATCGATCAGAACCTACGCAACACAATCTCAGAGGGACAGGGAGGGAGGTGTCAGTCACTCCCCAAAGCGGGAAGATCATATCTTCATGGGAATCACACAAGAGTGGGGGTGAGACCAGACTGTAAttaattaaCGTCCTTTCAACGATCCAAGTCcatgaaaaaaactaacacccctcccgtacctagcTTATAAAGTGCTGGGTCATCTAAGCTTTTCAGTCGAGAAAAAAACATTAGATCAGAAGAACAACTGGCAACACTTCTCAACCTGAGACTTACTTCAAGATGCTCGATCCTGGAGAGGTCTATGATGACCCTATTGACCCAATCGAGTTAGAGGCTGAACCCAGAGGAACCCCCATTGTCCCCAACATCTTGAGGAACTCTGACTACAATCTCAACTCTCCTTTGATAGAAGATCCTGCTAGACTAATGTTAGAATGGTTAAAAACAGGGAATAGACCTTATCGGATGACTCTAACAGACAATTGCTCCAGGTCTTTCAGAGTTTTGAAAGATTATTTCAAGAAGGTAGATTTGGGTTCTCTCAAGGTGGGCGGAATGGCTGCACAGTCAATGATTTCTCTCTGGTTATATGGTGCCCACTCTGAATCCAACAGGAGCCGGAGATGTATAACAGACTTGGCCCATTTCTATTCCAAGTCGTCCCCCATAGAGAAGCTGTTGAATCTCACGCTAGGAAATAGAGGGCTGAGAATCCCCCCAGAGGGAGTGTTAAGTTGCCTTGAGAGGGTTGATTATGATAATGCATTTGGAAGGTATCTTGCCAACACGTATTCCTCTTACTTGTTCTTCCATGTAATCACCTTATACATGAACGCCCTAGACTGGGATGAAGAAAAGACCATCCTAGCATTATGGAAAGATTTAACCTCAGTGGACATCGGGAAGGACTTGGTAAAGTTCAAAGACCAAATATGGGGACTGCTGATCGTGACAAAGGACTTTGTTTACTCCCAAAGTTCCAATTGTCTTTTTGACAGAAACTACACACTTATGCTAAAAGATCTTTTCTTGTCTCGCTTCAACTCCTTAATGGTCTTGCTCTCTCCCCCAGAGCCCCGATACTCAGATGACTTGATATCTCAACTATGCCAGCTGTACATTGCTGGGGATCAAGTCTTGTCTATGTGTGGAAACTCCGGCTATGAAGTCATCAAAATATTGGAGCCATATGTCGTGAATAGTTTAGTCCAGAGAGCAGAAAAGTTTAGGCCTCTCATTCATTCCTTGGGAGACTTTCCTGTATTTATAAAAGACAAGGTAAGTCAACTTGAAGAGACGTTCGGTCCCTGTGCAAGAAGGTTCTTTAGGGCTCTGGATCAATTCGACAACATACATGACTTGGTTTTTGTGTTTGGCTGTTACAGGCATTGGGGGCACCCATATATAGATTATCGAAAGGGTCTGTCAAAACTATATGATCAGGTTCACCTTAAAAAAATGATAGATAAGTCCTACCAGGAGTGCTTAGCAAGCGACCTAGCCAGGAGGATCCTTAGATGGGGTTTTGATAAGTACTCCAAGTGGTATCTGGATTCAAGATTCCTAGCCCGAGACCACCCCTTGACTCCTTATATCAAAACCCAAACATGGCCACCCAAACATATTGTAGACTTGGTGGGGGATACATGGCACAAGCTCCCGATCACGCAGATCTTTGAGATTCCTGAATCAATGGATCCGTCAGAAATATTGGATGACAAATCACATTCTTTCACCAGAACGAGACTAGCTTCTTGGCTGTCAGAAAACCGAGGGGGGCCTGTTCCTAGCGAAAAAGTTATTATCACGGCCCTGTCTAAGCCGCCTGTCAATCCCCGAGAGTTTCTGAGGTCTATAGACCTCGGAGGATTGCCAGATGAAGACTTGATAATTGGCCTCAAGCCAAAGGAACGGGAATTGAAGATTGAAGGTCGATTCTTTGCTCTAATGTCATGGAATCTAAGATTGTATTTTGTCATCACTGAAAAACTCTTGGCCAACTACATCTTGCCACTTTTTGACGCGCTGACTATGACAGACAACCTGAACAAGGTGTTTAAAAAGCTGATCGACAGGGTCACCGGGCAAGGGCTTTTGGACTATTCAAGGGTCACATATGCATTTCACCTGGACTATGAAAAGTGGAACAACCATCAAAGATTAGAGTCAACAGAGGATGTATTTTCTGTCCTAGATCAAGTGTTTGGATTGAAGAGAGTGTTTTCTAGAACACACGAGTTTTTTCAAAAGGCCTGGATCTATTATTCAGACAGATCAGACCTCATCGGGTTACGGGAGGATCAAATATACTGCTTAGATGCGTCCAACGGCCCAACCTGTTGGAATGGCCAGGATGGCGGGCTAGAAGGCTTACGGCAGAAGGGCTGGAGTCTAGTCAGCTTATTGATGATAGATAGAGAATCTCAAATCAGGAACACAAGAACCAAAATACTAGCTCAAGGAGACAACCAGGTTTTATGTCCGACATACATGTTGTCGCCAGGGCTATCTCAAGAGGGGCTCCTCTATGAATTGGAGAGAATATCAAGGAATGCACTTTCGATATACAGAGCCGTCGAGGAAGGGGCATCTAAGCTAGGGCTGATCATCAAGAAAGAAGAGACCATGTGTAGTTATGACTTCCTCATCTATGGAAAAACCCCTTTGTTTAGAGGTAACATATTGGTGCCTGAGTCCAAAAGATGGGCCAGAGTCTCTTGCGTCTCTAATGACCAAATAGTCAACCTCGCCAATATAATGTCGACAGTGTCCACCAATGCGCTAACAGTGGCACAACACTCTCAATCTTTGATCAAACCGATGAGGGATTTTCTGCTCATGTCAGTACAGGCAGTCTTTCACTACCTGCTATTTAGCCCAATCTTAAAGGGAAGAGTTTACAAGATTCTGAGCGCTGAAGGGGAGAGCTTTCTCCTAGCCATGTCAAGGATAATCTATCTAGATCCTTCTTTGGGAGGGATATCTGGAATGTCCCTCGGAAGATTCCATATACGACAGTTCTCAGACCCTGTCTCTGAAGGGTTATCCTTCTGGAGAGAGATCTGGTTAAGCTCCCAAGAGTCCTGGATTCACGCGTTGTGTCAAGAGGCTGGAAACCCAGATCTTGGAGAGAGAACACTCGAGAGCTTCACTCGCCTTCTAGAAGATCCGACCACCTTAAATATCAGAGGAGGGGCCAGTCCTACCATTCTACTCAAGGATGCAATCAGAAAGGCTTTATATGACGAGGTGGACAAGGTGGAAAATTCAGAGTTTCGAGAGGCAATCCTGTTGTCCAAGACCCATAGAGATAATTTTATACTCTTCTTAATATCTGTTGAGCCTCTGTTTCCTCGATTTCTCAGTGAGCTATTCAGTTCGTCTTTTTTGGGAATCCCCGAGTCAATCATTGGATTGATACAAAACTCCCGAACGATAAGAAGGCAGTTTAGAAAGAGTCTCTCAAAAACTTTAGAAGAATCCTTCTACAACTCAGAGATCCACGGGATTAGTCGGATGACCCAGACACCTCAGAGGGTTGGGGGGGTGTGGCCTTGCTCTTCAGAGAGGGCAGATCTACTTAGGGAGATCTCTTGGGGAAGAAAAGTGGTAGGCACGACAGTTCCTCACCCTTCTGAGATGTTGGGATTACTTCCCAAGTCCTCTATTTCTTGCACTTGTGGAGCAACAGGAGGAGGCAATCCTAGAGTTTCTGTATCAGTACTCCCGTCCTTTGATCAGTCATTTTTTTCACGAGGCCCCCTAAAGGGATACTTGGGCTCGTCCACCTCTATGTCGACCCAGCTATTCCATGCATGGGAAAAAGTCACTAATGTTCATGTGGTGAAGAGAGCTCTATCGTTAAAAGAATCTATAAACTGGTTCATTACTAGAGATTCCAACTTGGCTCAAGCTCTAATTAGGAACATTATGTCTCTGACAGGCCCTGATTTCCCTCTAGAGGAGGCCCCTGTCTTCAAAAGGACGGGGTCAGCCTTGCATAGGTTCAAGTCTGCCAGATACAGCGAAGGAGGGTATTCTTCTGTCTGCCCGAACCTCCTCTCTCATATTTCTGTTAGTACAGACACCATGTCTGATTTGACCCAAGACGGGAAGAACTACGATTTCATGTTCCAGCCATTGATGCTTTATGCACAGACATGGACATCAGAGCTGGTACAGAGAGACACAAGGCTAAGAGACTCTACGTTTCATTGGCACCTCCGATGCAACAGGTGTGTGAGACCCATTGACGACGTGACCCTGGAGACCTCTCAGATCTTCGAGTTTCCGGATGTGTCGAAAAGAATATCCAGAATGGTTTCTGGGGCTGTGCCTCACTTCCAGAGGCTTCCCGATATCCGTCTGAGACCAGGAGATTTTGAATCTCTAAGCGGTAGAGAAAAGTCTCACCATATCGGATCAGCTCAGGGGCTCTTATACTCAATCTTAGTGGCAATTCACGACTCAGGATACAATGATGGAACCATCTTCCCTGTCAACATATACGGCAAGGTTTCCCCTAGAGACTATTTGAGAGGGCTCGCAAGGGGAGTATTGATAGGATCCTCGATTTGCTTCTTGACAAGAATGACAAATATCAATATTAATAGACCTCTTGAATTGGTCTCAGGGGTAATCTCATATATTCTCCTGAGGCTAGATAACCATCCCTCCTTGTACATAATGCTCAGAGAACCGTCTCTTAGAGGAGAGATATTTTCTATCCCTCAGAAAATCCCCGCCGCTTATCCAACCACTATGAAAGAAGGCAACAGATCAATCTTGTGTTATCTCCAACATGTGCTACGCTATGAGCGAGAGATAATCACGGCGTCTCCAGAGAATGACTGGCTATGGATCTTTTCAGACTTTAGAAGTGCCAAAATGACGTACCTATCCCTCATTACTTACCAGTCTCATCTTCTACTCCAGAGGGTTGAGAGAAACCTATCTAAGAGTATGAGAGATAACCTGCGACAATTGAGTTCTTTGATGAGGCAGGTGCTGGGCGGGCACGGAGAAGATACCTTAGAGTCAGACGACAACATTCAACGACTGCTAAAAGACTCTTTACGAAGGACAAGATGGGTGGATCAAGAGGTGCGCCATGCAGCTAGAACCATGACTGGAGATTACAGCCCCAACAAGAAGGTGTCCCGTAAGGTAGGATGTTCAGAATGGGTCTGCTCTGCTCAACAGGTTGCAGTCTCTACCTCAGCAAACCCGGCCCCTGTCTCGGAGCTTGACATAAGGGCCCTCTCTAAGAGGTTCCAGAACCCTTTGATCTCGGGCTTGAGAGTGGTTCAGTGGGCAACCGGTGCTCATTATAAGCTTAAGCCTATTCTAGATGATCTCAATGTTTTCCCATCTCTCTGCCTTGTAGTTGGGGACGGGTCAGGGGGGATATCAAGGGCAGTCCTCAACATGTTTCCAGATGCCAAGCTTGTGTTCAACAGTCTTTTAGAGGTGAATGACCTGATGGCTTCCGGAACACATCCACTGCCTCCTTCAGCAATCATGAGGGGAGGAAATGATATCGTCTCCAGAGTGATAGATCTTGACTCAATCTGGGAAAAACCGTCCGACTTGAGAAACTTGGCAACCTGGAAATACTTCCAGTCAGTCCAAAAGCAGGTCAACATGTCCTATGACCTCATTATTTGCGATGCAGAAGTTACTGACATTGCATCTATCAACCGGATCACCCTGTTAATGTCCGATTTTGCATTGTCTATAGATGGACCACTCTATTTGGTCTTCAAAACTTATGGGACTATGCTAGTAAATCCAAACTACAAGGCTATTCAACACCTGTCAAGAGCGTTCCCCTCGGTCACAGGGTTTATCACCCAAGTAACTTCGTCTTTTTCATCTGAGCTCTACCTCCGATTCTCCAAACGAGGGAAGTTTTTCAGAGATGCTGAGTACTTGACCTCTTCCACCCTTCGAGAAATGAGCCTTGTGTTATTCAATTGTAGCAGCCCCAAGAGTGAGATGCAGAGAGCTCGTTCCTTGAACTATCAGGATCTTGTGAGAGGATTTCCTGAAGAAATCATATCAAATCCTTACAATGAGATGATCATAACTCTGATTGACAGTGATGTAGAATCTTTTCTAGTCCACAAGATGGTTGATGATCTTGAGTTACAGAGGGGAACTCTGTCTAAAGTGGCTATCATTATAGCCATCATGATAGTTTTCTCCAACAGAGTCTTCAACGTTTCCAAACCCCTAACTGACCCCTCGTTCTATCCACCGTCTGATCCCAAAATCCTGAGGCACTTCAACATATGTTGCAGTACTATGATGTATCTATCTACTGCTTTAGGTGACGTCCCTAGCTTCGCAAGACTTCACGACCTGTATAACAGACCTATAACTTATTACTTCAGAAAGCAAGTCATTCGAGGGAACGTTTATCTATCTTGGAGTTGGTCCAACGACACCTCAGTGTTCAAAAGGGTAGCCTGTAATTCTAGCCTGAGTCTGTCATCTCACTGGATCAGGTTGATTTACAAGATAGTGAAGACTACCAGACTCGTTGGCAGCATCAAGGATCTATCCAGAGAAGTGGAAAGACACCTTCATAGGTACAACAGGTGGATCACCCTAGAGGATATCAGATCTAGATCATCCCTACTAGACTACAGTTGCCTGTGAACCGGATACTCCTGGAAGCCTGCCCATGCTAAGACTCTTGTGTGATGTATCTTGAAAAAAACAAGATCCTAAATCTGAACCTTTGGTTGTTTGATTGTTTTTCTCAtttttgttgtttatttgttaagcgt。
在一个实施方式中，核酸包括SEQ ID NO：11的序列，如下所示(RABV载体：CoravaxV3-South Africa(S1-VSVG-E26))：
ACGCTTAACAACCAGATCAAAGAAAAAACAGACATTGTCAATTGCAAAGCAAAAATGTAACACCCCTACAATGGATGCCGACAAGATTGTATTCAAAGTCAATAATCAGGTGGTCTCTTTGAAGCCTGAGATTATCGTGGATCAATATGAGTACAAGTACCCTGCCATCAAAGATTTGAAAAAGCCCTGTATAACCCTAGGAAAGGCTCCCGATTTAAATAAAGCATACAAGTCAGTTTTGTCAGGCATGAGCGCCGCCAAACTTAATCCTGACGATGTATGTTCCTATTTGGCAGCGGCAATGCAGTTTTTTGAGGGGACATGTCCGGAAGACTGGACCAGCTATGGAATTGTGATTGCACGAAAAGGAGATAAGATCACCCCAGGTTCTCTGGTGGAGATAAAACGTACTGATGTAGAAGGGAATTGGGCTCTGACAGGAGGCATGGAACTGACAAGAGACCCCACTGTCCCTGAGCATGCGTCCTTAGTCGGTCTTCTCTTGAGTCTGTATAGGTTGAGCAAAATATCCGGGCAAAACACTGGTAACTATAAGACAAACATTGCAGACAGGATAGAGCAGATTTTTGAGACAGCCCCTTTTGTTAAAATCGTGGAACACCATACTCTAATGACAACTCACAAaATGTGTGCTAATTGGAGTACTATACCAAACTTCAGATTTTTGGCCGGAACCTATGACATGTTTTTCTCCCGGATTGAGCATCTATATTCAGCAATCAGAGTGGGCACAGTTGTCACTGCTTATGAAGACTGTTCAGGACTGGTATCATTTACTGGGTTCATAAAACAAATCAATCTCACCGCTAGAGAGGCAATACTATATTTCTTCCACAAGAACTTTGAGGAAGAGATAAGAAGAATGTTTGAGCCAGGGCAGGAGACAGCTGTTCCTCACTCTTATTTCATCCACTTCCGTTCACTAGGCTTGAGTGGGAAATCTCCTTATTCATCAAATGCTGTTGGTCACGTGTTCAATCTCATTCACTTTGTAGGATGCTATATGGGTCAAGTCAGATCCCTAAATGCAACGGTTATTGCTGCATGTGCTCCTCATGAAATGTCTGTTCTAGGGGGCTATCTGGGAGAGGAATTCTTCGGGAAAGGGACATTTGAAAGAAGATTCTTCAGAGATGAGAAAGAACTTCAAGAATACGAGGCGGCTGAACTGACAAAGACTGACGTAGCACTGGCAGATGATGGAACTGTCAACTCTGACGACGAGGACTACTTTTCAGGTGAAACCAGAAGTCCGGAGGCTGTTTATACTCGAATCATGATGAATGGAGGTCGACTAAAGAGATCTCACATACGGAGATATGTCTCAGTCAGTTCCAATCATCAAGCCCGTCCAAACTCATTCGCCGAGTTTCTAAACAAGACATATTCGAGTGACTCATAAcatgaaaaaaactaacacccctcccGTACGCCACCATGTTCGTGTTTCTGGTGCTGCTGCCTCTGGTGAGCTCCCAGTGCGTGAACTTCACCACAAGGACCCAGCTGCCCCCTGCCTATACCAATTCCTTCACACGGGGCGTGTACTATCCCGACAAGGTGTTCCGGAGCAGCGTGCTGCACTCCACACAGGATCTGTTTCTGCCTTTCTTTTCTAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAATGGCACAAAGCGGTTCGCCAATCCAGTGCTGCCCTTTAACGATGGCGTGTACTTCGCCTCCACCGAGAAGTCTAACATCATCAGAGGCTGGATCTTTGGCACCACACTGGACAGCAAGACACAGTCCCTGCTGATCGTGAACAATGCCACCAACGTGGTCATCAAGGTGTGCGAGTTCCAGTTTTGTAATGATCCATTCCTGGGCGTGTACTATCACAAGAACAATAAGTCTTGGATGGAGAGCGAGTTTCGCGTGTATTCCTCTGCCAACAATTGCACATTTGAGTACGTGTCCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAATTTCAAGAACCTGAGGGAGTTCGTGTTTAAGAATATCGATGGCTACTTCAAAATCTACTCCAAGCACACCCCAATCAACCTGGTGCGCGGCCTGCCACAGGGCTTCTCTGCCCTGGAGCCACTGGTGGATCTGCCCATCGGCATCAACATCACCCGGTTTCAGACACTGCTGGCCCTGCACAGAAGCTACCTGACACCAGGCGACAGCTCCTCTGGATGGACCGCAGGAGCAGCAGCCTACTATGTGGGCTATCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAATGGCACCATCACAGACGCCGTGGATTGCGCCCTGGATCCCCTGTCTGAGACCAAGTGTACACTGAAGAGCTTTACCGTGGAGAAGGGCATCTATCAGACAAGCAATTTCAGGGTGCAGCCTACCGAGTCCATCGTGCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAATATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGAAGGGCTTTAACTGTTATTTCCCTCTGCAGAGCTACGGCTTCCAGCCAACATATGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACCTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGACTGACCGGAACAGGCGTGCTGACCGAGTCCAACAAGAAGTTCCTGCCTTTTCAGCAGTTCGGCAGGGACATCGCAGATACCACAGACGCCGTGCGCGACCCTCAGACCCTGGAGATCCTGGACATCACACCATGCTCCTTCGGCGGCGTGTCTGTGATCACACCAGGCACCAATACAAGCAACCAGGTGGCCGTGCTGTATCAGGGCGTGAATTGTACCGAGGTGCCAGTGGCAATCCACGCAGATCAGCTGACCCCTACATGGCGGGTGTACTCTACCGGCAGCAACGTGTTCCAGACAAGAGCCGGATGCCTGATCGGAGCAGAGCACGTGAACAATAGCTATGAGTGCGACATCCCTATCGGCGCCGGCATCTGTGCCTCCTACCAGACCCAGACAAACTCCCCAAGGTCTGTGGGCGATACAGGCCTGTCCAAGAATCCAATCGAGCTGGTAGAGGGCTGGTTCAGCAGTTGGAAAAGCTCCATCGCCTCCTTTTTCTTTATCATCGGCCTGATCATCGGACTGTTCCTGGTGCTCCGCGTGGGTATCCACCTGTGCATCAAGCTGAAGCACACCAAGAAAAGACAGATTTATACAGACATCGAGATGAACCGCCTGGGAAAGTGAGCTAGCCATGAAAAAAACTAACACCCCTCCTTTCGAACCATCCCAAACATGAGCAAGATCTTTGTCAATCCTAGTGCTATTAGAGCCGGTCTGGCCGATCTTGAGATGGCTGAAGAAACTGTTGATCTGATCAATAGAAATATCGAAGACAATCAGGCTCATCTCCAAGGGGAACCCATAGAGGTGGACAATCTCCCTGAGGATATGGGGCGACTTCACCTGGATGATGGAAAATCGCCCAACCATGGTGAGATAGCCAAGGTGGGAGAAGGCAAGTATCGAGAGGACTTTCAGATGGATGAAGGAGAGGATCCTAGCTTCCTGTTCCAGTCATACCTGGAAAATGTTGGAGTCCAAATAGTCAGACAAATGAGGTCAGGAGAGAGATTTCTCAAGATATGGTCACAGACCGTAGAAGAGATTATATCCTATGTCGCGGTCAACTTTCCCAACCCTCCAGGAAAGTCTTCAGAGGATAAATCAACCCAGACTACTGGCCGAGAGCTCAAGAAGGAGACAACACCCACTCCTTCTCAGAGAGAAAGCCAATCATCGAAAGCCAGGATGGCGGCTCAAATTGCTTCTGGCCCTCCAGCCCTTGAATGGTCGGCTACCAATGAAGAGGATGATCTATCAGTGGAGGCTGAGATCGCTCACCAGATTGCAGAAAGTTTCTCCAAAAAATATAAGTTTCCCTCTCGATCCTCAGGGATACTCTTGTATAATTTTGAGCAATTGAAAATGAACCTTGATGATATAGTTAAAGAGGCAAAAAATGTACCAGGTGTGACCCGTTTAGCCCATGACGGGTCCAAACTCCCCCTAAGATGTGTACTGGGATGGGTCGCTTTGGCCAACTCTAAGAAATTCCAGTTGTTAGTCGAATCCGACAAGCTGAGTAAAATCATGCAAGATGACTTGAATCGCTATACATCTTGCTAACCGAACCTCTCCCCTCAGTCCCTCTAGACAATAAAATCCGAGATGTCCCAAAGTCAACATGAAAAAAACAGGCAACACCACTGATAAAATGAACCTCCTACGTAAGATAGTGAAAAACCGCAGGGACGAGGACACTCAAAAATCCTCTCCCGCGTCAGCCCCTCTGGATGACGATGACTTGTGGCTTCCACCCCCTGAATACGTCCCGCTGAAAGAACTTACAGGCAAGAAGAACATGAGGAACTTTTGTATCAACGGAAGGGTTAAAGTGTGTAGCCCGAATGGTTACTCGTTCAGGATCCTGCGGCACATTCTGAAATCATTCGACGAGATATATTCTGGGAATCATAGGATGATCGGGTTAGTCAAAGTGGTTATTGGACTGGCTTTGTCAGGATCTCCAGTCCCTGAGGGCCTGAACTGGGTATACAAATTGAGGAGAACCTTTATCTTCCAGTGGGCTGATTCCAGGGGCCCTCTTGAAGGGGAGGAGTTGGAATACTCTCAGGAGATCACTTGGGATGATGATACTGAGTTCGTCGGATTGCAAATAAGAGTGATTGCAAAACAGTGTCATATCCAGGGCAGAGTCTGGTGTATCAACATGAACCCGAGAGCATGTCAACTATGGTCTGACATGTCTCTTCAGACACAAAGGTCCGAAGAGGACAAAGATTCCTCTCTGCTTCTAGAATAATCAGATTATATCCCGCAAATTTATCACTTGTTTACCTCTGGAGGAGAGAACATATGGGCTCAACTCCAACCCTTGGGAGCAATATAACAAAAAACATGTTATGGTGCCATTAAACCGCTGCATTTCATCAAAGTCAAGTTGATTACCTTTACATTTTGATCCTCTTGGATGTGAAAAAAACTATTAACATCCCTCAAAAGACcccgggAAAGATGGTTCCTCAGGCTCTCCTGTTTGTACCCCTTCTGGTTTTTCCATTGTGTTTTGGGAAATTCCCTATTTACACGATACCAGACAAGCTTGGTCCCTGGAGTCCGATTGACATACATCACCTCAGCTGCCCAAACAATTTGGTAGTGGAGGACGAAGGATGCACCAACCTGTCAGGGTTCTCCTACATGGAACTTAAAGTTGGATACATCTTAGCCATAAAAGTGAACGGGTTCACTTGCACAGGCGTTGTGACGGAGGCTGAAACCTACACTAACTTCGTTGGTTATGTCACAACCACGTTCAAAAGAAAGCATTTCCGCCCAACACCAGATGCATGTAGAGCCGCGTACAACTGGAAGATGGCCGGTGACCCCAGATATGAAGAGTCTCTACACAATCCGTACCCTGACTACCGCTGGCTTCGAACTGTAAAAACCACCAAGGAGTCTCTCGTTATCATATCTCCAAGTGTGGCAGATTTGGACCCATATGACAGATCCCTTCACTCGAGGGTCTTCCCTAGCGGGAAGTGCTCAGGAGTAGCGGTGTCTTCTACCTACTGCTCCACTAACCACGATTACACCATTTGGATGCCCGAGAATCCGAGACTAGGGATGTCTTGTGACATTTTTACCAATAGTAGAGGGAAGAGAGCATCCAAAGGGAGTGAGACTTGCGGCTTTGTAGATGAAAGAGGCCTATATAAGTCTTTAAAAGGAGCATGCAAACTCAAGTTATGTGGAGTTCTAGGACTTAGACTTATGGATGGAACATGGGTCTCGATGCAAACATCAAATGAAACCAAATGGTGCCCTCCCGATAAGTTGGTGAACCTGCACGACTTTCGCTCAGACGAAATTGAGCACCTTGTTGTAGAGGAGTTGGTCAGGAAGAGAGAGGAGTGTCTGGATGCACTAGAGTCCATCATGACAACCAAGTCAGTGAGTTTCAGACGTCTCAGTCATTTAAGAAAACTTGTCCCTGGGTTTGGAAAAGCATATACCATATTCAACAAGACCTTGATGGAAGCCGATGCTCACTACAAGTCAGTCgagACTTGGAATGAGATCCTCCCTTCAAAAGGGTGTTTAAGAGTTGGGGGGAGGTGTCATCCTCATGTGAACGGGGTGTTTTTCAATGGTATAATATTAGGACCTGACGGCAATGTCTTAATCCCAGAGATGCAATCATCCCTCCTCCAGCAACATATGGAGTTGTTGGAATCCTCGGTTATCCCCCTTGTGCACCCCCTGGCAGACCCGTCTACCGTTTTCAAGGACGGTGACGAGGCTGAGGATTTTGTTGAAGTTCACCTTCCCGATGTGCACAATCAGGTCTCAGGAGTTGACTTGGGTCTCCCGAACTGGGGGAAGTATGTATTACTGAGTGCAGGGGCCCTGACTGCCTTGATGTTGATAATTTTCCTGATGACATGTTGTAGAAGAGTCAATCGATCAGAACCTACGCAACACAATCTCAGAGGGACAGGGAGGGAGGTGTCAGTCACTCCCCAAAGCGGGAAGATCATATCTTCATGGGAATCACACAAGAGTGGGGGTGAGACCAGACTGTAAttaattaaCGTCCTTTCAACGATCCAAGTCcatgaaaaaaactaacacccctcccgtacctagcTTATAAAGTGCTGGGTCATCTAAGCTTTTCAGTCGAGAAAAAAACATTAGATCAGAAGAACAACTGGCAACACTTCTCAACCTGAGACTTACTTCAAGATGCTCGATCCTGGAGAGGTCTATGATGACCCTATTGACCCAATCGAGTTAGAGGCTGAACCCAGAGGAACCCCCATTGTCCCCAACATCTTGAGGAACTCTGACTACAATCTCAACTCTCCTTTGATAGAAGATCCTGCTAGACTAATGTTAGAATGGTTAAAAACAGGGAATAGACCTTATCGGATGACTCTAACAGACAATTGCTCCAGGTCTTTCAGAGTTTTGAAAGATTATTTCAAGAAGGTAGATTTGGGTTCTCTCAAGGTGGGCGGAATGGCTGCACAGTCAATGATTTCTCTCTGGTTATATGGTGCCCACTCTGAATCCAACAGGAGCCGGAGATGTATAACAGACTTGGCCCATTTCTATTCCAAGTCGTCCCCCATAGAGAAGCTGTTGAATCTCACGCTAGGAAATAGAGGGCTGAGAATCCCCCCAGAGGGAGTGTTAAGTTGCCTTGAGAGGGTTGATTATGATAATGCATTTGGAAGGTATCTTGCCAACACGTATTCCTCTTACTTGTTCTTCCATGTAATCACCTTATACATGAACGCCCTAGACTGGGATGAAGAAAAGACCATCCTAGCATTATGGAAAGATTTAACCTCAGTGGACATCGGGAAGGACTTGGTAAAGTTCAAAGACCAAATATGGGGACTGCTGATCGTGACAAAGGACTTTGTTTACTCCCAAAGTTCCAATTGTCTTTTTGACAGAAACTACACACTTATGCTAAAAGATCTTTTCTTGTCTCGCTTCAACTCCTTAATGGTCTTGCTCTCTCCCCCAGAGCCCCGATACTCAGATGACTTGATATCTCAACTATGCCAGCTGTACATTGCTGGGGATCAAGTCTTGTCTATGTGTGGAAACTCCGGCTATGAAGTCATCAAAATATTGGAGCCATATGTCGTGAATAGTTTAGTCCAGAGAGCAGAAAAGTTTAGGCCTCTCATTCATTCCTTGGGAGACTTTCCTGTATTTATAAAAGACAAGGTAAGTCAACTTGAAGAGACGTTCGGTCCCTGTGCAAGAAGGTTCTTTAGGGCTCTGGATCAATTCGACAACATACATGACTTGGTTTTTGTGTTTGGCTGTTACAGGCATTGGGGGCACCCATATATAGATTATCGAAAGGGTCTGTCAAAACTATATGATCAGGTTCACCTTAAAAAAATGATAGATAAGTCCTACCAGGAGTGCTTAGCAAGCGACCTAGCCAGGAGGATCCTTAGATGGGGTTTTGATAAGTACTCCAAGTGGTATCTGGATTCAAGATTCCTAGCCCGAGACCACCCCTTGACTCCTTATATCAAAACCCAAACATGGCCACCCAAACATATTGTAGACTTGGTGGGGGATACATGGCACAAGCTCCCGATCACGCAGATCTTTGAGATTCCTGAATCAATGGATCCGTCAGAAATATTGGATGACAAATCACATTCTTTCACCAGAACGAGACTAGCTTCTTGGCTGTCAGAAAACCGAGGGGGGCCTGTTCCTAGCGAAAAAGTTATTATCACGGCCCTGTCTAAGCCGCCTGTCAATCCCCGAGAGTTTCTGAGGTCTATAGACCTCGGAGGATTGCCAGATGAAGACTTGATAATTGGCCTCAAGCCAAAGGAACGGGAATTGAAGATTGAAGGTCGATTCTTTGCTCTAATGTCATGGAATCTAAGATTGTATTTTGTCATCACTGAAAAACTCTTGGCCAACTACATCTTGCCACTTTTTGACGCGCTGACTATGACAGACAACCTGAACAAGGTGTTTAAAAAGCTGATCGACAGGGTCACCGGGCAAGGGCTTTTGGACTATTCAAGGGTCACATATGCATTTCACCTGGACTATGAAAAGTGGAACAACCATCAAAGATTAGAGTCAACAGAGGATGTATTTTCTGTCCTAGATCAAGTGTTTGGATTGAAGAGAGTGTTTTCTAGAACACACGAGTTTTTTCAAAAGGCCTGGATCTATTATTCAGACAGATCAGACCTCATCGGGTTACGGGAGGATCAAATATACTGCTTAGATGCGTCCAACGGCCCAACCTGTTGGAATGGCCAGGATGGCGGGCTAGAAGGCTTACGGCAGAAGGGCTGGAGTCTAGTCAGCTTATTGATGATAGATAGAGAATCTCAAATCAGGAACACAAGAACCAAAATACTAGCTCAAGGAGACAACCAGGTTTTATGTCCGACATACATGTTGTCGCCAGGGCTATCTCAAGAGGGGCTCCTCTATGAATTGGAGAGAATATCAAGGAATGCACTTTCGATATACAGAGCCGTCGAGGAAGGGGCATCTAAGCTAGGGCTGATCATCAAGAAAGAAGAGACCATGTGTAGTTATGACTTCCTCATCTATGGAAAAACCCCTTTGTTTAGAGGTAACATATTGGTGCCTGAGTCCAAAAGATGGGCCAGAGTCTCTTGCGTCTCTAATGACCAAATAGTCAACCTCGCCAATATAATGTCGACAGTGTCCACCAATGCGCTAACAGTGGCACAACACTCTCAATCTTTGATCAAACCGATGAGGGATTTTCTGCTCATGTCAGTACAGGCAGTCTTTCACTACCTGCTATTTAGCCCAATCTTAAAGGGAAGAGTTTACAAGATTCTGAGCGCTGAAGGGGAGAGCTTTCTCCTAGCCATGTCAAGGATAATCTATCTAGATCCTTCTTTGGGAGGGATATCTGGAATGTCCCTCGGAAGATTCCATATACGACAGTTCTCAGACCCTGTCTCTGAAGGGTTATCCTTCTGGAGAGAGATCTGGTTAAGCTCCCAAGAGTCCTGGATTCACGCGTTGTGTCAAGAGGCTGGAAACCCAGATCTTGGAGAGAGAACACTCGAGAGCTTCACTCGCCTTCTAGAAGATCCGACCACCTTAAATATCAGAGGAGGGGCCAGTCCTACCATTCTACTCAAGGATGCAATCAGAAAGGCTTTATATGACGAGGTGGACAAGGTGGAAAATTCAGAGTTTCGAGAGGCAATCCTGTTGTCCAAGACCCATAGAGATAATTTTATACTCTTCTTAATATCTGTTGAGCCTCTGTTTCCTCGATTTCTCAGTGAGCTATTCAGTTCGTCTTTTTTGGGAATCCCCGAGTCAATCATTGGATTGATACAAAACTCCCGAACGATAAGAAGGCAGTTTAGAAAGAGTCTCTCAAAAACTTTAGAAGAATCCTTCTACAACTCAGAGATCCACGGGATTAGTCGGATGACCCAGACACCTCAGAGGGTTGGGGGGGTGTGGCCTTGCTCTTCAGAGAGGGCAGATCTACTTAGGGAGATCTCTTGGGGAAGAAAAGTGGTAGGCACGACAGTTCCTCACCCTTCTGAGATGTTGGGATTACTTCCCAAGTCCTCTATTTCTTGCACTTGTGGAGCAACAGGAGGAGGCAATCCTAGAGTTTCTGTATCAGTACTCCCGTCCTTTGATCAGTCATTTTTTTCACGAGGCCCCCTAAAGGGATACTTGGGCTCGTCCACCTCTATGTCGACCCAGCTATTCCATGCATGGGAAAAAGTCACTAATGTTCATGTGGTGAAGAGAGCTCTATCGTTAAAAGAATCTATAAACTGGTTCATTACTAGAGATTCCAACTTGGCTCAAGCTCTAATTAGGAACATTATGTCTCTGACAGGCCCTGATTTCCCTCTAGAGGAGGCCCCTGTCTTCAAAAGGACGGGGTCAGCCTTGCATAGGTTCAAGTCTGCCAGATACAGCGAAGGAGGGTATTCTTCTGTCTGCCCGAACCTCCTCTCTCATATTTCTGTTAGTACAGACACCATGTCTGATTTGACCCAAGACGGGAAGAACTACGATTTCATGTTCCAGCCATTGATGCTTTATGCACAGACATGGACATCAGAGCTGGTACAGAGAGACACAAGGCTAAGAGACTCTACGTTTCATTGGCACCTCCGATGCAACAGGTGTGTGAGACCCATTGACGACGTGACCCTGGAGACCTCTCAGATCTTCGAGTTTCCGGATGTGTCGAAAAGAATATCCAGAATGGTTTCTGGGGCTGTGCCTCACTTCCAGAGGCTTCCCGATATCCGTCTGAGACCAGGAGATTTTGAATCTCTAAGCGGTAGAGAAAAGTCTCACCATATCGGATCAGCTCAGGGGCTCTTATACTCAATCTTAGTGGCAATTCACGACTCAGGATACAATGATGGAACCATCTTCCCTGTCAACATATACGGCAAGGTTTCCCCTAGAGACTATTTGAGAGGGCTCGCAAGGGGAGTATTGATAGGATCCTCGATTTGCTTCTTGACAAGAATGACAAATATCAATATTAATAGACCTCTTGAATTGGTCTCAGGGGTAATCTCATATATTCTCCTGAGGCTAGATAACCATCCCTCCTTGTACATAATGCTCAGAGAACCGTCTCTTAGAGGAGAGATATTTTCTATCCCTCAGAAAATCCCCGCCGCTTATCCAACCACTATGAAAGAAGGCAACAGATCAATCTTGTGTTATCTCCAACATGTGCTACGCTATGAGCGAGAGATAATCACGGCGTCTCCAGAGAATGACTGGCTATGGATCTTTTCAGACTTTAGAAGTGCCAAAATGACGTACCTATCCCTCATTACTTACCAGTCTCATCTTCTACTCCAGAGGGTTGAGAGAAACCTATCTAAGAGTATGAGAGATAACCTGCGACAATTGAGTTCTTTGATGAGGCAGGTGCTGGGCGGGCACGGAGAAGATACCTTAGAGTCAGACGACAACATTCAACGACTGCTAAAAGACTCTTTACGAAGGACAAGATGGGTGGATCAAGAGGTGCGCCATGCAGCTAGAACCATGACTGGAGATTACAGCCCCAACAAGAAGGTGTCCCGTAAGGTAGGATGTTCAGAATGGGTCTGCTCTGCTCAACAGGTTGCAGTCTCTACCTCAGCAAACCCGGCCCCTGTCTCGGAGCTTGACATAAGGGCCCTCTCTAAGAGGTTCCAGAACCCTTTGATCTCGGGCTTGAGAGTGGTTCAGTGGGCAACCGGTGCTCATTATAAGCTTAAGCCTATTCTAGATGATCTCAATGTTTTCCCATCTCTCTGCCTTGTAGTTGGGGACGGGTCAGGGGGGATATCAAGGGCAGTCCTCAACATGTTTCCAGATGCCAAGCTTGTGTTCAACAGTCTTTTAGAGGTGAATGACCTGATGGCTTCCGGAACACATCCACTGCCTCCTTCAGCAATCATGAGGGGAGGAAATGATATCGTCTCCAGAGTGATAGATCTTGACTCAATCTGGGAAAAACCGTCCGACTTGAGAAACTTGGCAACCTGGAAATACTTCCAGTCAGTCCAAAAGCAGGTCAACATGTCCTATGACCTCATTATTTGCGATGCAGAAGTTACTGACATTGCATCTATCAACCGGATCACCCTGTTAATGTCCGATTTTGCATTGTCTATAGATGGACCACTCTATTTGGTCTTCAAAACTTATGGGACTATGCTAGTAAATCCAAACTACAAGGCTATTCAACACCTGTCAAGAGCGTTCCCCTCGGTCACAGGGTTTATCACCCAAGTAACTTCGTCTTTTTCATCTGAGCTCTACCTCCGATTCTCCAAACGAGGGAAGTTTTTCAGAGATGCTGAGTACTTGACCTCTTCCACCCTTCGAGAAATGAGCCTTGTGTTATTCAATTGTAGCAGCCCCAAGAGTGAGATGCAGAGAGCTCGTTCCTTGAACTATCAGGATCTTGTGAGAGGATTTCCTGAAGAAATCATATCAAATCCTTACAATGAGATGATCATAACTCTGATTGACAGTGATGTAGAATCTTTTCTAGTCCACAAGATGGTTGATGATCTTGAGTTACAGAGGGGAACTCTGTCTAAAGTGGCTATCATTATAGCCATCATGATAGTTTTCTCCAACAGAGTCTTCAACGTTTCCAAACCCCTAACTGACCCCTCGTTCTATCCACCGTCTGATCCCAAAATCCTGAGGCACTTCAACATATGTTGCAGTACTATGATGTATCTATCTACTGCTTTAGGTGACGTCCCTAGCTTCGCAAGACTTCACGACCTGTATAACAGACCTATAACTTATTACTTCAGAAAGCAAGTCATTCGAGGGAACGTTTATCTATCTTGGAGTTGGTCCAACGACACCTCAGTGTTCAAAAGGGTAGCCTGTAATTCTAGCCTGAGTCTGTCATCTCACTGGATCAGGTTGATTTACAAGATAGTGAAGACTACCAGACTCGTTGGCAGCATCAAGGATCTATCCAGAGAAGTGGAAAGACACCTTCATAGGTACAACAGGTGGATCACCCTAGAGGATATCAGATCTAGATCATCCCTACTAGACTACAGTTGCCTGTGAACCGGATACTCCTGGAAGCCTGCCCATGCTAAGACTCTTGTGTGATGTATCTTGAAAAAAACAAGATCCTAAATCTGAACCTTTGGTTGTTTGATTGTTTTTCTCAtttttgttgtttatttgttaagcgt。
在一个实施方式中，核酸包括SEQ ID NO：12的序列，如下所示(RABV载体：CoravaxV4-China(S1-RABVG-T2A-P))：
ACGCTTAACAACCAGATCAAAGAAAAAACAGACATTGTCAATTGCAAAGCAAAAATGTAACACCCCTACAATGGATGCCGACAAGATTGTATTCAAAGTCAATAATCAGGTGGTCTCTTTGAAGCCTGAGATTATCGTGGATCAATATGAGTACAAGTACCCTGCCATCAAAGATTTGAAAAAGCCCTGTATAACCCTAGGAAAGGCTCCCGATTTAAATAAAGCATACAAGTCAGTTTTGTCAGGCATGAGCGCCGCCAAACTTAATCCTGACGATGTATGTTCCTATTTGGCAGCGGCAATGCAGTTTTTTGAGGGGACATGTCCGGAAGACTGGACCAGCTATGGAATTGTGATTGCACGAAAAGGAGATAAGATCACCCCAGGTTCTCTGGTGGAGATAAAACGTACTGATGTAGAAGGGAATTGGGCTCTGACAGGAGGCATGGAACTGACAAGAGACCCCACTGTCCCTGAGCATGCGTCCTTAGTCGGTCTTCTCTTGAGTCTGTATAGGTTGAGCAAAATATCCGGGCAAAACACTGGTAACTATAAGACAAACATTGCAGACAGGATAGAGCAGATTTTTGAGACAGCCCCTTTTGTTAAAATCGTGGAACACCATACTCTAATGACAACTCACAAaATGTGTGCTAATTGGAGTACTATACCAAACTTCAGATTTTTGGCCGGAACCTATGACATGTTTTTCTCCCGGATTGAGCATCTATATTCAGCAATCAGAGTGGGCACAGTTGTCACTGCTTATGAAGACTGTTCAGGACTGGTATCATTTACTGGGTTCATAAAACAAATCAATCTCACCGCTAGAGAGGCAATACTATATTTCTTCCACAAGAACTTTGAGGAAGAGATAAGAAGAATGTTTGAGCCAGGGCAGGAGACAGCTGTTCCTCACTCTTATTTCATCCACTTCCGTTCACTAGGCTTGAGTGGGAAATCTCCTTATTCATCAAATGCTGTTGGTCACGTGTTCAATCTCATTCACTTTGTAGGATGCTATATGGGTCAAGTCAGATCCCTAAATGCAACGGTTATTGCTGCATGTGCTCCTCATGAAATGTCTGTTCTAGGGGGCTATCTGGGAGAGGAATTCTTCGGGAAAGGGACATTTGAAAGAAGATTCTTCAGAGATGAGAAAGAACTTCAAGAATACGAGGCGGCTGAACTGACAAAGACTGACGTAGCACTGGCAGATGATGGAACTGTCAACTCTGACGACGAGGACTACTTTTCAGGTGAAACCAGAAGTCCGGAGGCTGTTTATACTCGAATCATGATGAATGGAGGTCGACTAAAGAGATCTCACATACGGAGATATGTCTCAGTCAGTTCCAATCATCAAGCCCGTCCAAACTCATTCGCCGAGTTTCTAAACAAGACATATTCGAGTGACTCATAAcatgaaaaaaactaacacccctcccgtacgGCCACCATGTTCGTGTTTCTGGTGCTGCTGCCTCTGGTGAGCTCCCAGTGCGTGAACCTGACCACAAGGACCCAGCTGCCCCCTGCCTATACCAATTCCTTCACACGGGGCGTGTACTATCCCGACAAGGTGTTCCGGAGCAGCGTGCTGCACTCCACACAGGATCTGTTTCTGCCTTTCTTTTCTAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAATGGCACAAAGCGGTTCGACAATCCAGTGCTGCCCTTTAACGATGGCGTGTACTTCGCCTCCACCGAGAAGTCTAACATCATCAGAGGCTGGATCTTTGGCACCACACTGGACAGCAAGACACAGTCCCTGCTGATCGTGAACAATGCCACCAACGTGGTCATCAAGGTGTGCGAGTTCCAGTTTTGTAATGATCCATTCCTGGGCGTGTACTATCACAAGAACAATAAGTCTTGGATGGAGAGCGAGTTTCGCGTGTATTCCTCTGCCAACAATTGCACATTTGAGTACGTGTCCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAATTTCAAGAACCTGAGGGAGTTCGTGTTTAAGAATATCGATGGCTACTTCAAAATCTACTCCAAGCACACCCCAATCAACCTGGTGCGCGACCTGCCACAGGGCTTCTCTGCCCTGGAGCCACTGGTGGATCTGCCCATCGGCATCAACATCACCCGGTTTCAGACACTGCTGGCCCTGCACAGAAGCTACCTGACACCAGGCGACAGCTCCTCTGGATGGACCGCAGGAGCAGCAGCCTACTATGTGGGCTATCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAATGGCACCATCACAGACGCCGTGGATTGCGCCCTGGATCCCCTGTCTGAGACCAAGTGTACACTGAAGAGCTTTACCGTGGAGAAGGGCATCTATCAGACAAGCAATTTCAGGGTGCAGCCTACCGAGTCCATCGTGCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAAGATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGGAGGGCTTTAACTGTTATTTCCCTCTGCAGAGCTACGGCTTCCAGCCAACAAACGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACCTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGACTGACCGGAACAGGCGTGCTGACCGAGTCCAACAAGAAGTTCCTGCCTTTTCAGCAGTTCGGCAGGGACATCGCAGATACCACAGACGCCGTGCGCGACCCTCAGACCCTGGAGATCCTGGACATCACACCATGCTCCTTCGGCGGCGTGTCTGTGATCACACCAGGCACCAATACAAGCAACCAGGTGGCCGTGCTGTATCAGGACGTGAATTGTACCGAGGTGCCAGTGGCAATCCACGCAGATCAGCTGACCCCTACATGGCGGGTGTACTCTACCGGCAGCAACGTGTTCCAGACAAGAGCCGGATGCCTGATCGGAGCAGAGCACGTGAACAATAGCTATGAGTGCGACATCCCTATCGGCGCCGGCATCTGTGCCTCCTACCAGACCCAGACAAACTCCCCAAGGTCTGTGGGAGATGAGGCCGAAGACTTTGTGGAAGTCCACCTGCCTGATGTGCATAACCAGGTGTCTGGCGTCGACCTGGGACTGCCAAATTGGGGCAAGTACGTGCTGCTGAGTGCTGGAGCACTGACTGCCCTGATGCTGATCATTTTCCTGATGACCTGCTGTCGGCGCGTGAACAGAAGTGAGCCCACTCAGCACAATCTGCGAGGAACCGGGAGAGAAGTGTCAGTCACACCTCAGAGCGGGAAAATCATTAGTAGTTGGGAATCACATAAAAGCGGGGGCGAGACCAGGCTGggatccggctccggcgagggcaggggaagtctactaacatgcggggacgtggaggaaaatcccggccccATGAGCAAGATCTTTGTCAATCCTAGTGCTATTAGAGCCGGTCTGGCCGATCTTGAGATGGCTGAAGAAACTGTTGATCTGATCAATAGAAATATCGAAGACAATCAGGCTCATCTCCAAGGGGAACCCATAGAGGTGGACAATCTCCCTGAGGATATGGGGCGACTTCACCTGGATGATGGAAAATCGCCCAACCATGGTGAGATAGCCAAGGTGGGAGAAGGCAAGTATCGAGAGGACTTTCAGATGGATGAAGGAGAGGATCCTAGCTTCCTGTTCCAGTCATACCTGGAAAATGTTGGAGTCCAAATAGTCAGACAAATGAGGTCAGGAGAGAGATTTCTCAAGATATGGTCACAGACCGTAGAAGAGATTATATCCTATGTCGCGGTCAACTTTCCCAACCCTCCAGGAAAGTCTTCAGAGGATAAATCAACCCAGACTACTGGCCGAGAGCTCAAGAAGGAGACAACACCCACTCCTTCTCAGAGAGAAAGCCAATCATCGAAAGCCAGGATGGCGGCTCAAATTGCTTCTGGCCCTCCAGCCCTTGAATGGTCGGCTACCAATGAAGAGGATGATCTATCAGTGGAGGCTGAGATCGCTCACCAGATTGCAGAAAGTTTCTCCAAAAAATATAAGTTTCCCTCTCGATCCTCAGGGATACTCTTGTATAATTTTGAGCAATTGAAAATGAACCTTGATGATATAGTTAAAGAGGCAAAAAATGTACCAGGTGTGACCCGTTTAGCCCATGACGGGTCCAAACTCCCCCTAAGATGTGTACTGGGATGGGTCGCTTTGGCCAACTCTAAGAAATTCCAGTTGTTAGTCGAATCCGACAAGCTGAGTAAAATCATGCAAGATGACTTGAATCGCTATACATCTTGCTAACCGAACCTCTCCCCTCAGTCCCTCTAGACAATAAAATCCGAGATGTCCCAAAGTCAACATGAAAAAAACAGGCAACACCACTGATAAAATGAACCTCCTACGTAAGATAGTGAAAAACCGCAGGGACGAGGACACTCAAAAATCCTCTCCCGCGTCAGCCCCTCTGGATGACGATGACTTGTGGCTTCCACCCCCTGAATACGTCCCGCTGAAAGAACTTACAGGCAAGAAGAACATGAGGAACTTTTGTATCAACGGAAGGGTTAAAGTGTGTAGCCCGAATGGTTACTCGTTCAGGATCCTGCGGCACATTCTGAAATCATTCGACGAGATATATTCTGGGAATCATAGGATGATCGGGTTAGTCAAAGTGGTTATTGGACTGGCTTTGTCAGGATCTCCAGTCCCTGAGGGCCTGAACTGGGTATACAAATTGAGGAGAACCTTTATCTTCCAGTGGGCTGATTCCAGGGGCCCTCTTGAAGGGGAGGAGTTGGAATACTCTCAGGAGATCACTTGGGATGATGATACTGAGTTCGTCGGATTGCAAATAAGAGTGATTGCAAAACAGTGTCATATCCAGGGCAGAGTCTGGTGTATCAACATGAACCCGAGAGCATGTCAACTATGGTCTGACATGTCTCTTCAGACACAAAGGTCCGAAGAGGACAAAGATTCCTCTCTGCTTCTAGAATAATCAGATTATATCCCGCAAATTTATCACTTGTTTACCTCTGGAGGAGAGAACATATGGGCTCAACTCCAACCCTTGGGAGCAATATAACAAAAAACATGTTATGGTGCCATTAAACCGCTGCATTTCATCAAAGTCAAGTTGATTACCTTTACATTTTGATCCTCTTGGATGTGAAAAAAACTATTAACATCCCTCAAAAGACCccgggAAAGATGGTTCCTCAGGCTCTCCTGTTTGTACCCCTTCTGGTTTTTCCATTGTGTTTTGGGAAATTCCCTATTTACACGATACCAGACAAGCTTGGTCCCTGGAGTCCGATTGACATACATCACCTCAGCTGCCCAAACAATTTGGTAGTGGAGGACGAAGGATGCACCAACCTGTCAGGGTTCTCCTACATGGAACTTAAAGTTGGATACATCTTAGCCATAAAAGTGAACGGGTTCACTTGCACAGGCGTTGTGACGGAGGCTGAAACCTACACTAACTTCGTTGGTTATGTCACAACCACGTTCAAAAGAAAGCATTTCCGCCCAACACCAGATGCATGTAGAGCCGCGTACAACTGGAAGATGGCCGGTGACCCCAGATATGAAGAGTCTCTACACAATCCGTACCCTGACTACCGCTGGCTTCGAACTGTAAAAACCACCAAGGAGTCTCTCGTTATCATATCTCCAAGTGTGGCAGATTTGGACCCATATGACAGATCCCTTCACTCGAGGGTCTTCCCTAGCGGGAAGTGCTCAGGAGTAGCGGTGTCTTCTACCTACTGCTCCACTAACCACGATTACACCATTTGGATGCCCGAGAATCCGAGACTAGGGATGTCTTGTGACATTTTTACCAATAGTAGAGGGAAGAGAGCATCCAAAGGGAGTGAGACTTGCGGCTTTGTAGATGAAAGAGGCCTATATAAGTCTTTAAAAGGAGCATGCAAACTCAAGTTATGTGGAGTTCTAGGACTTAGACTTATGGATGGAACATGGGTCTCGATGCAAACATCAAATGAAACCAAATGGTGCCCTCCCGATAAGTTGGTGAACCTGCACGACTTTCGCTCAGACGAAATTGAGCACCTTGTTGTAGAGGAGTTGGTCAGGAAGAGAGAGGAGTGTCTGGATGCACTAGAGTCCATCATGACAACCAAGTCAGTGAGTTTCAGACGTCTCAGTCATTTAAGAAAACTTGTCCCTGGGTTTGGAAAAGCATATACCATATTCAACAAGACCTTGATGGAAGCCGATGCTCACTACAAGTCAGTCgagACTTGGAATGAGATCCTCCCTTCAAAAGGGTGTTTAAGAGTTGGGGGGAGGTGTCATCCTCATGTGAACGGGGTGTTTTTCAATGGTATAATATTAGGACCTGACGGCAATGTCTTAATCCCAGAGATGCAATCATCCCTCCTCCAGCAACATATGGAGTTGTTGGAATCCTCGGTTATCCCCCTTGTGCACCCCCTGGCAGACCCGTCTACCGTTTTCAAGGACGGTGACGAGGCTGAGGATTTTGTTGAAGTTCACCTTCCCGATGTGCACAATCAGGTCTCAGGAGTTGACTTGGGTCTCCCGAACTGGGGGAAGTATGTATTACTGAGTGCAGGGGCCCTGACTGCCTTGATGTTGATAATTTTCCTGATGACATGTTGTAGAAGAGTCAATCGATCAGAACCTACGCAACACAATCTCAGAGGGACAGGGAGGGAGGTGTCAGTCACTCCCCAAAGCGGGAAGATCATATCTTCATGGGAATCACACAAGAGTGGGGGTGAGACCAGACTGTAAttaattaaCGTCCTTTCAACGATCCAAGTCcatgaaaaaaactaacacccctcccgtacctagcTTATAAAGTGCTGGGTCATCTAAGCTTTTCAGTCGAGAAAAAAACATTAGATCAGAAGAACAACTGGCAACACTTCTCAACCTGAGACTTACTTCAAGATGCTCGATCCTGGAGAGGTCTATGATGACCCTATTGACCCAATCGAGTTAGAGGCTGAACCCAGAGGAACCCCCATTGTCCCCAACATCTTGAGGAACTCTGACTACAATCTCAACTCTCCTTTGATAGAAGATCCTGCTAGACTAATGTTAGAATGGTTAAAAACAGGGAATAGACCTTATCGGATGACTCTAACAGACAATTGCTCCAGGTCTTTCAGAGTTTTGAAAGATTATTTCAAGAAGGTAGATTTGGGTTCTCTCAAGGTGGGCGGAATGGCTGCACAGTCAATGATTTCTCTCTGGTTATATGGTGCCCACTCTGAATCCAACAGGAGCCGGAGATGTATAACAGACTTGGCCCATTTCTATTCCAAGTCGTCCCCCATAGAGAAGCTGTTGAATCTCACGCTAGGAAATAGAGGGCTGAGAATCCCCCCAGAGGGAGTGTTAAGTTGCCTTGAGAGGGTTGATTATGATAATGCATTTGGAAGGTATCTTGCCAACACGTATTCCTCTTACTTGTTCTTCCATGTAATCACCTTATACATGAACGCCCTAGACTGGGATGAAGAAAAGACCATCCTAGCATTATGGAAAGATTTAACCTCAGTGGACATCGGGAAGGACTTGGTAAAGTTCAAAGACCAAATATGGGGACTGCTGATCGTGACAAAGGACTTTGTTTACTCCCAAAGTTCCAATTGTCTTTTTGACAGAAACTACACACTTATGCTAAAAGATCTTTTCTTGTCTCGCTTCAACTCCTTAATGGTCTTGCTCTCTCCCCCAGAGCCCCGATACTCAGATGACTTGATATCTCAACTATGCCAGCTGTACATTGCTGGGGATCAAGTCTTGTCTATGTGTGGAAACTCCGGCTATGAAGTCATCAAAATATTGGAGCCATATGTCGTGAATAGTTTAGTCCAGAGAGCAGAAAAGTTTAGGCCTCTCATTCATTCCTTGGGAGACTTTCCTGTATTTATAAAAGACAAGGTAAGTCAACTTGAAGAGACGTTCGGTCCCTGTGCAAGAAGGTTCTTTAGGGCTCTGGATCAATTCGACAACATACATGACTTGGTTTTTGTGTTTGGCTGTTACAGGCATTGGGGGCACCCATATATAGATTATCGAAAGGGTCTGTCAAAACTATATGATCAGGTTCACCTTAAAAAAATGATAGATAAGTCCTACCAGGAGTGCTTAGCAAGCGACCTAGCCAGGAGGATCCTTAGATGGGGTTTTGATAAGTACTCCAAGTGGTATCTGGATTCAAGATTCCTAGCCCGAGACCACCCCTTGACTCCTTATATCAAAACCCAAACATGGCCACCCAAACATATTGTAGACTTGGTGGGGGATACATGGCACAAGCTCCCGATCACGCAGATCTTTGAGATTCCTGAATCAATGGATCCGTCAGAAATATTGGATGACAAATCACATTCTTTCACCAGAACGAGACTAGCTTCTTGGCTGTCAGAAAACCGAGGGGGGCCTGTTCCTAGCGAAAAAGTTATTATCACGGCCCTGTCTAAGCCGCCTGTCAATCCCCGAGAGTTTCTGAGGTCTATAGACCTCGGAGGATTGCCAGATGAAGACTTGATAATTGGCCTCAAGCCAAAGGAACGGGAATTGAAGATTGAAGGTCGATTCTTTGCTCTAATGTCATGGAATCTAAGATTGTATTTTGTCATCACTGAAAAACTCTTGGCCAACTACATCTTGCCACTTTTTGACGCGCTGACTATGACAGACAACCTGAACAAGGTGTTTAAAAAGCTGATCGACAGGGTCACCGGGCAAGGGCTTTTGGACTATTCAAGGGTCACATATGCATTTCACCTGGACTATGAAAAGTGGAACAACCATCAAAGATTAGAGTCAACAGAGGATGTATTTTCTGTCCTAGATCAAGTGTTTGGATTGAAGAGAGTGTTTTCTAGAACACACGAGTTTTTTCAAAAGGCCTGGATCTATTATTCAGACAGATCAGACCTCATCGGGTTACGGGAGGATCAAATATACTGCTTAGATGCGTCCAACGGCCCAACCTGTTGGAATGGCCAGGATGGCGGGCTAGAAGGCTTACGGCAGAAGGGCTGGAGTCTAGTCAGCTTATTGATGATAGATAGAGAATCTCAAATCAGGAACACAAGAACCAAAATACTAGCTCAAGGAGACAACCAGGTTTTATGTCCGACATACATGTTGTCGCCAGGGCTATCTCAAGAGGGGCTCCTCTATGAATTGGAGAGAATATCAAGGAATGCACTTTCGATATACAGAGCCGTCGAGGAAGGGGCATCTAAGCTAGGGCTGATCATCAAGAAAGAAGAGACCATGTGTAGTTATGACTTCCTCATCTATGGAAAAACCCCTTTGTTTAGAGGTAACATATTGGTGCCTGAGTCCAAAAGATGGGCCAGAGTCTCTTGCGTCTCTAATGACCAAATAGTCAACCTCGCCAATATAATGTCGACAGTGTCCACCAATGCGCTAACAGTGGCACAACACTCTCAATCTTTGATCAAACCGATGAGGGATTTTCTGCTCATGTCAGTACAGGCAGTCTTTCACTACCTGCTATTTAGCCCAATCTTAAAGGGAAGAGTTTACAAGATTCTGAGCGCTGAAGGGGAGAGCTTTCTCCTAGCCATGTCAAGGATAATCTATCTAGATCCTTCTTTGGGAGGGATATCTGGAATGTCCCTCGGAAGATTCCATATACGACAGTTCTCAGACCCTGTCTCTGAAGGGTTATCCTTCTGGAGAGAGATCTGGTTAAGCTCCCAAGAGTCCTGGATTCACGCGTTGTGTCAAGAGGCTGGAAACCCAGATCTTGGAGAGAGAACACTCGAGAGCTTCACTCGCCTTCTAGAAGATCCGACCACCTTAAATATCAGAGGAGGGGCCAGTCCTACCATTCTACTCAAGGATGCAATCAGAAAGGCTTTATATGACGAGGTGGACAAGGTGGAAAATTCAGAGTTTCGAGAGGCAATCCTGTTGTCCAAGACCCATAGAGATAATTTTATACTCTTCTTAATATCTGTTGAGCCTCTGTTTCCTCGATTTCTCAGTGAGCTATTCAGTTCGTCTTTTTTGGGAATCCCCGAGTCAATCATTGGATTGATACAAAACTCCCGAACGATAAGAAGGCAGTTTAGAAAGAGTCTCTCAAAAACTTTAGAAGAATCCTTCTACAACTCAGAGATCCACGGGATTAGTCGGATGACCCAGACACCTCAGAGGGTTGGGGGGGTGTGGCCTTGCTCTTCAGAGAGGGCAGATCTACTTAGGGAGATCTCTTGGGGAAGAAAAGTGGTAGGCACGACAGTTCCTCACCCTTCTGAGATGTTGGGATTACTTCCCAAGTCCTCTATTTCTTGCACTTGTGGAGCAACAGGAGGAGGCAATCCTAGAGTTTCTGTATCAGTACTCCCGTCCTTTGATCAGTCATTTTTTTCACGAGGCCCCCTAAAGGGATACTTGGGCTCGTCCACCTCTATGTCGACCCAGCTATTCCATGCATGGGAAAAAGTCACTAATGTTCATGTGGTGAAGAGAGCTCTATCGTTAAAAGAATCTATAAACTGGTTCATTACTAGAGATTCCAACTTGGCTCAAGCTCTAATTAGGAACATTATGTCTCTGACAGGCCCTGATTTCCCTCTAGAGGAGGCCCCTGTCTTCAAAAGGACGGGGTCAGCCTTGCATAGGTTCAAGTCTGCCAGATACAGCGAAGGAGGGTATTCTTCTGTCTGCCCGAACCTCCTCTCTCATATTTCTGTTAGTACAGACACCATGTCTGATTTGACCCAAGACGGGAAGAACTACGATTTCATGTTCCAGCCATTGATGCTTTATGCACAGACATGGACATCAGAGCTGGTACAGAGAGACACAAGGCTAAGAGACTCTACGTTTCATTGGCACCTCCGATGCAACAGGTGTGTGAGACCCATTGACGACGTGACCCTGGAGACCTCTCAGATCTTCGAGTTTCCGGATGTGTCGAAAAGAATATCCAGAATGGTTTCTGGGGCTGTGCCTCACTTCCAGAGGCTTCCCGATATCCGTCTGAGACCAGGAGATTTTGAATCTCTAAGCGGTAGAGAAAAGTCTCACCATATCGGATCAGCTCAGGGGCTCTTATACTCAATCTTAGTGGCAATTCACGACTCAGGATACAATGATGGAACCATCTTCCCTGTCAACATATACGGCAAGGTTTCCCCTAGAGACTATTTGAGAGGGCTCGCAAGGGGAGTATTGATAGGATCCTCGATTTGCTTCTTGACAAGAATGACAAATATCAATATTAATAGACCTCTTGAATTGGTCTCAGGGGTAATCTCATATATTCTCCTGAGGCTAGATAACCATCCCTCCTTGTACATAATGCTCAGAGAACCGTCTCTTAGAGGAGAGATATTTTCTATCCCTCAGAAAATCCCCGCCGCTTATCCAACCACTATGAAAGAAGGCAACAGATCAATCTTGTGTTATCTCCAACATGTGCTACGCTATGAGCGAGAGATAATCACGGCGTCTCCAGAGAATGACTGGCTATGGATCTTTTCAGACTTTAGAAGTGCCAAAATGACGTACCTATCCCTCATTACTTACCAGTCTCATCTTCTACTCCAGAGGGTTGAGAGAAACCTATCTAAGAGTATGAGAGATAACCTGCGACAATTGAGTTCTTTGATGAGGCAGGTGCTGGGCGGGCACGGAGAAGATACCTTAGAGTCAGACGACAACATTCAACGACTGCTAAAAGACTCTTTACGAAGGACAAGATGGGTGGATCAAGAGGTGCGCCATGCAGCTAGAACCATGACTGGAGATTACAGCCCCAACAAGAAGGTGTCCCGTAAGGTAGGATGTTCAGAATGGGTCTGCTCTGCTCAACAGGTTGCAGTCTCTACCTCAGCAAACCCGGCCCCTGTCTCGGAGCTTGACATAAGGGCCCTCTCTAAGAGGTTCCAGAACCCTTTGATCTCGGGCTTGAGAGTGGTTCAGTGGGCAACCGGTGCTCATTATAAGCTTAAGCCTATTCTAGATGATCTCAATGTTTTCCCATCTCTCTGCCTTGTAGTTGGGGACGGGTCAGGGGGGATATCAAGGGCAGTCCTCAACATGTTTCCAGATGCCAAGCTTGTGTTCAACAGTCTTTTAGAGGTGAATGACCTGATGGCTTCCGGAACACATCCACTGCCTCCTTCAGCAATCATGAGGGGAGGAAATGATATCGTCTCCAGAGTGATAGATCTTGACTCAATCTGGGAAAAACCGTCCGACTTGAGAAACTTGGCAACCTGGAAATACTTCCAGTCAGTCCAAAAGCAGGTCAACATGTCCTATGACCTCATTATTTGCGATGCAGAAGTTACTGACATTGCATCTATCAACCGGATCACCCTGTTAATGTCCGATTTTGCATTGTCTATAGATGGACCACTCTATTTGGTCTTCAAAACTTATGGGACTATGCTAGTAAATCCAAACTACAAGGCTATTCAACACCTGTCAAGAGCGTTCCCCTCGGTCACAGGGTTTATCACCCAAGTAACTTCGTCTTTTTCATCTGAGCTCTACCTCCGATTCTCCAAACGAGGGAAGTTTTTCAGAGATGCTGAGTACTTGACCTCTTCCACCCTTCGAGAAATGAGCCTTGTGTTATTCAATTGTAGCAGCCCCAAGAGTGAGATGCAGAGAGCTCGTTCCTTGAACTATCAGGATCTTGTGAGAGGATTTCCTGAAGAAATCATATCAAATCCTTACAATGAGATGATCATAACTCTGATTGACAGTGATGTAGAATCTTTTCTAGTCCACAAGATGGTTGATGATCTTGAGTTACAGAGGGGAACTCTGTCTAAAGTGGCTATCATTATAGCCATCATGATAGTTTTCTCCAACAGAGTCTTCAACGTTTCCAAACCCCTAACTGACCCCTCGTTCTATCCACCGTCTGATCCCAAAATCCTGAGGCACTTCAACATATGTTGCAGTACTATGATGTATCTATCTACTGCTTTAGGTGACGTCCCTAGCTTCGCAAGACTTCACGACCTGTATAACAGACCTATAACTTATTACTTCAGAAAGCAAGTCATTCGAGGGAACGTTTATCTATCTTGGAGTTGGTCCAACGACACCTCAGTGTTCAAAAGGGTAGCCTGTAATTCTAGCCTGAGTCTGTCATCTCACTGGATCAGGTTGATTTACAAGATAGTGAAGACTACCAGACTCGTTGGCAGCATCAAGGATCTATCCAGAGAAGTGGAAAGACACCTTCATAGGTACAACAGGTGGATCACCCTAGAGGATATCAGATCTAGATCATCCCTACTAGACTACAGTTGCCTGTGAACCGGATACTCCTGGAAGCCTGCCCATGCTAAGACTCTTGTGTGATGTATCTTGAAAAAAACAAGATCCTAAATCTGAACCTTTGGTTGTTTGATTGTTTTTCTCAtttttgttgtttatttgttaagcgt
在一个实施方式中，核酸包括SEQ ID NO：13的序列，如下所示(RABV载体：CoravaxV4 South Africa)：
ACGCTTAACAACCAGATCAAAGAAAAAACAGACATTGTCAATTGCAAAGCAAAAATGTAACACCCCTACAATGGATGCCGACAAGATTGTATTCAAAGTCAATAATCAGGTGGTCTCTTTGAAGCCTGAGATTATCGTGGATCAATATGAGTACAAGTACCCTGCCATCAAAGATTTGAAAAAGCCCTGTATAACCCTAGGAAAGGCTCCCGATTTAAATAAAGCATACAAGTCAGTTTTGTCAGGCATGAGCGCCGCCAAACTTAATCCTGACGATGTATGTTCCTATTTGGCAGCGGCAATGCAGTTTTTTGAGGGGACATGTCCGGAAGACTGGACCAGCTATGGAATTGTGATTGCACGAAAAGGAGATAAGATCACCCCAGGTTCTCTGGTGGAGATAAAACGTACTGATGTAGAAGGGAATTGGGCTCTGACAGGAGGCATGGAACTGACAAGAGACCCCACTGTCCCTGAGCATGCGTCCTTAGTCGGTCTTCTCTTGAGTCTGTATAGGTTGAGCAAAATATCCGGGCAAAACACTGGTAACTATAAGACAAACATTGCAGACAGGATAGAGCAGATTTTTGAGACAGCCCCTTTTGTTAAAATCGTGGAACACCATACTCTAATGACAACTCACAAaATGTGTGCTAATTGGAGTACTATACCAAACTTCAGATTTTTGGCCGGAACCTATGACATGTTTTTCTCCCGGATTGAGCATCTATATTCAGCAATCAGAGTGGGCACAGTTGTCACTGCTTATGAAGACTGTTCAGGACTGGTATCATTTACTGGGTTCATAAAACAAATCAATCTCACCGCTAGAGAGGCAATACTATATTTCTTCCACAAGAACTTTGAGGAAGAGATAAGAAGAATGTTTGAGCCAGGGCAGGAGACAGCTGTTCCTCACTCTTATTTCATCCACTTCCGTTCACTAGGCTTGAGTGGGAAATCTCCTTATTCATCAAATGCTGTTGGTCACGTGTTCAATCTCATTCACTTTGTAGGATGCTATATGGGTCAAGTCAGATCCCTAAATGCAACGGTTATTGCTGCATGTGCTCCTCATGAAATGTCTGTTCTAGGGGGCTATCTGGGAGAGGAATTCTTCGGGAAAGGGACATTTGAAAGAAGATTCTTCAGAGATGAGAAAGAACTTCAAGAATACGAGGCGGCTGAACTGACAAAGACTGACGTAGCACTGGCAGATGATGGAACTGTCAACTCTGACGACGAGGACTACTTTTCAGGTGAAACCAGAAGTCCGGAGGCTGTTTATACTCGAATCATGATGAATGGAGGTCGACTAAAGAGATCTCACATACGGAGATATGTCTCAGTCAGTTCCAATCATCAAGCCCGTCCAAACTCATTCGCCGAGTTTCTAAACAAGACATATTCGAGTGACTCATAAcatgaaaaaaactaacacccctcccgtacgGCCACCATGTTCGTGTTTCTGGTGCTGCTGCCTCTGGTGAGCTCCCAGTGCGTGAACTTCACCACAAGGACCCAGCTGCCCCCTGCCTATACCAATTCCTTCACACGGGGCGTGTACTATCCCGACAAGGTGTTCCGGAGCAGCGTGCTGCACTCCACACAGGATCTGTTTCTGCCTTTCTTTTCTAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAATGGCACAAAGCGGTTCGCCAATCCAGTGCTGCCCTTTAACGATGGCGTGTACTTCGCCTCCACCGAGAAGTCTAACATCATCAGAGGCTGGATCTTTGGCACCACACTGGACAGCAAGACACAGTCCCTGCTGATCGTGAACAATGCCACCAACGTGGTCATCAAGGTGTGCGAGTTCCAGTTTTGTAATGATCCATTCCTGGGCGTGTACTATCACAAGAACAATAAGTCTTGGATGGAGAGCGAGTTTCGCGTGTATTCCTCTGCCAACAATTGCACATTTGAGTACGTGTCCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAATTTCAAGAACCTGAGGGAGTTCGTGTTTAAGAATATCGATGGCTACTTCAAAATCTACTCCAAGCACACCCCAATCAACCTGGTGCGCGGCCTGCCACAGGGCTTCTCTGCCCTGGAGCCACTGGTGGATCTGCCCATCGGCATCAACATCACCCGGTTTCAGACACTGCTGGCCCTGCACAGAAGCTACCTGACACCAGGCGACAGCTCCTCTGGATGGACCGCAGGAGCAGCAGCCTACTATGTGGGCTATCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAATGGCACCATCACAGACGCCGTGGATTGCGCCCTGGATCCCCTGTCTGAGACCAAGTGTACACTGAAGAGCTTTACCGTGGAGAAGGGCATCTATCAGACAAGCAATTTCAGGGTGCAGCCTACCGAGTCCATCGTGCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAATATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGAAGGGCTTTAACTGTTATTTCCCTCTGCAGAGCTACGGCTTCCAGCCAACATATGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACCTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGACTGACCGGAACAGGCGTGCTGACCGAGTCCAACAAGAAGTTCCTGCCTTTTCAGCAGTTCGGCAGGGACATCGCAGATACCACAGACGCCGTGCGCGACCCTCAGACCCTGGAGATCCTGGACATCACACCATGCTCCTTCGGCGGCGTGTCTGTGATCACACCAGGCACCAATACAAGCAACCAGGTGGCCGTGCTGTATCAGGGCGTGAATTGTACCGAGGTGCCAGTGGCAATCCACGCAGATCAGCTGACCCCTACATGGCGGGTGTACTCTACCGGCAGCAACGTGTTCCAGACAAGAGCCGGATGCCTGATCGGAGCAGAGCACGTGAACAATAGCTATGAGTGCGACATCCCTATCGGCGCCGGCATCTGTGCCTCCTACCAGACCCAGACAAACTCCCCAGAATCAAGCGTGATTCCTCTGGTCCATCCACTGGCAGATCCCTCCACAGTGTTCAAAGACGGAGATGAGGCCGAAGACTTTGTGGAAGTCCACCTGCCTGATGTGCATAACCAGGTGTCTGGCGTCGACCTGGGACTGCCAAATTGGGGCAAGTACGTGCTGCTGAGTGCTGGAGCACTGACTGCCCTGATGCTGATCATTTTCCTGATGACCTGCTGTCGGCGCGTGAACAGAAGTGAGCCCACTCAGCACAATCTGCGAGGAACCGGGAGAGAAGTGTCAGTCACACCTCAGAGCGGGAAAATCATTAGTAGTTGGGAATCACATAAAAGCGGGGGCGAGACCAGGCTGggatccggctccggcgagggcaggggaagtctactaacatgcggggacgtggaggaaaatcccggccccATGAGCAAGATCTTTGTCAATCCTAGTGCTATTAGAGCCGGTCTGGCCGATCTTGAGATGGCTGAAGAAACTGTTGATCTGATCAATAGAAATATCGAAGACAATCAGGCTCATCTCCAAGGGGAACCCATAGAGGTGGACAATCTCCCTGAGGATATGGGGCGACTTCACCTGGATGATGGAAAATCGCCCAACCATGGTGAGATAGCCAAGGTGGGAGAAGGCAAGTATCGAGAGGACTTTCAGATGGATGAAGGAGAGGATCCTAGCTTCCTGTTCCAGTCATACCTGGAAAATGTTGGAGTCCAAATAGTCAGACAAATGAGGTCAGGAGAGAGATTTCTCAAGATATGGTCACAGACCGTAGAAGAGATTATATCCTATGTCGCGGTCAACTTTCCCAACCCTCCAGGAAAGTCTTCAGAGGATAAATCAACCCAGACTACTGGCCGAGAGCTCAAGAAGGAGACAACACCCACTCCTTCTCAGAGAGAAAGCCAATCATCGAAAGCCAGGATGGCGGCTCAAATTGCTTCTGGCCCTCCAGCCCTTGAATGGTCGGCTACCAATGAAGAGGATGATCTATCAGTGGAGGCTGAGATCGCTCACCAGATTGCAGAAAGTTTCTCCAAAAAATATAAGTTTCCCTCTCGATCCTCAGGGATACTCTTGTATAATTTTGAGCAATTGAAAATGAACCTTGATGATATAGTTAAAGAGGCAAAAAATGTACCAGGTGTGACCCGTTTAGCCCATGACGGGTCCAAACTCCCCCTAAGATGTGTACTGGGATGGGTCGCTTTGGCCAACTCTAAGAAATTCCAGTTGTTAGTCGAATCCGACAAGCTGAGTAAAATCATGCAAGATGACTTGAATCGCTATACATCTTGCTAACCGAACCTCTCCCCTCAGTCCCTCTAGACAATAAAATCCGAGATGTCCCAAAGTCAACATGAAAAAAACAGGCAACACCACTGATAAAATGAACCTCCTACGTAAGATAGTGAAAAACCGCAGGGACGAGGACACTCAAAAATCCTCTCCCGCGTCAGCCCCTCTGGATGACGATGACTTGTGGCTTCCACCCCCTGAATACGTCCCGCTGAAAGAACTTACAGGCAAGAAGAACATGAGGAACTTTTGTATCAACGGAAGGGTTAAAGTGTGTAGCCCGAATGGTTACTCGTTCAGGATCCTGCGGCACATTCTGAAATCATTCGACGAGATATATTCTGGGAATCATAGGATGATCGGGTTAGTCAAAGTGGTTATTGGACTGGCTTTGTCAGGATCTCCAGTCCCTGAGGGCCTGAACTGGGTATACAAATTGAGGAGAACCTTTATCTTCCAGTGGGCTGATTCCAGGGGCCCTCTTGAAGGGGAGGAGTTGGAATACTCTCAGGAGATCACTTGGGATGATGATACTGAGTTCGTCGGATTGCAAATAAGAGTGATTGCAAAACAGTGTCATATCCAGGGCAGAGTCTGGTGTATCAACATGAACCCGAGAGCATGTCAACTATGGTCTGACATGTCTCTTCAGACACAAAGGTCCGAAGAGGACAAAGATTCCTCTCTGCTTCTAGAATAATCAGATTATATCCCGCAAATTTATCACTTGTTTACCTCTGGAGGAGAGAACATATGGGCTCAACTCCAACCCTTGGGAGCAATATAACAAAAAACATGTTATGGTGCCATTAAACCGCTGCATTTCATCAAAGTCAAGTTGATTACCTTTACATTTTGATCCTCTTGGATGTGAAAAAAACTATTAACATCCCTCAAAAGACCccgggAAAGATGGTTCCTCAGGCTCTCCTGTTTGTACCCCTTCTGGTTTTTCCATTGTGTTTTGGGAAATTCCCTATTTACACGATACCAGACAAGCTTGGTCCCTGGAGTCCGATTGACATACATCACCTCAGCTGCCCAAACAATTTGGTAGTGGAGGACGAAGGATGCACCAACCTGTCAGGGTTCTCCTACATGGAACTTAAAGTTGGATACATCTTAGCCATAAAAGTGAACGGGTTCACTTGCACAGGCGTTGTGACGGAGGCTGAAACCTACACTAACTTCGTTGGTTATGTCACAACCACGTTCAAAAGAAAGCATTTCCGCCCAACACCAGATGCATGTAGAGCCGCGTACAACTGGAAGATGGCCGGTGACCCCAGATATGAAGAGTCTCTACACAATCCGTACCCTGACTACCGCTGGCTTCGAACTGTAAAAACCACCAAGGAGTCTCTCGTTATCATATCTCCAAGTGTGGCAGATTTGGACCCATATGACAGATCCCTTCACTCGAGGGTCTTCCCTAGCGGGAAGTGCTCAGGAGTAGCGGTGTCTTCTACCTACTGCTCCACTAACCACGATTACACCATTTGGATGCCCGAGAATCCGAGACTAGGGATGTCTTGTGACATTTTTACCAATAGTAGAGGGAAGAGAGCATCCAAAGGGAGTGAGACTTGCGGCTTTGTAGATGAAAGAGGCCTATATAAGTCTTTAAAAGGAGCATGCAAACTCAAGTTATGTGGAGTTCTAGGACTTAGACTTATGGATGGAACATGGGTCTCGATGCAAACATCAAATGAAACCAAATGGTGCCCTCCCGATAAGTTGGTGAACCTGCACGACTTTCGCTCAGACGAAATTGAGCACCTTGTTGTAGAGGAGTTGGTCAGGAAGAGAGAGGAGTGTCTGGATGCACTAGAGTCCATCATGACAACCAAGTCAGTGAGTTTCAGACGTCTCAGTCATTTAAGAAAACTTGTCCCTGGGTTTGGAAAAGCATATACCATATTCAACAAGACCTTGATGGAAGCCGATGCTCACTACAAGTCAGTCgagACTTGGAATGAGATCCTCCCTTCAAAAGGGTGTTTAAGAGTTGGGGGGAGGTGTCATCCTCATGTGAACGGGGTGTTTTTCAATGGTATAATATTAGGACCTGACGGCAATGTCTTAATCCCAGAGATGCAATCATCCCTCCTCCAGCAACATATGGAGTTGTTGGAATCCTCGGTTATCCCCCTTGTGCACCCCCTGGCAGACCCGTCTACCGTTTTCAAGGACGGTGACGAGGCTGAGGATTTTGTTGAAGTTCACCTTCCCGATGTGCACAATCAGGTCTCAGGAGTTGACTTGGGTCTCCCGAACTGGGGGAAGTATGTATTACTGAGTGCAGGGGCCCTGACTGCCTTGATGTTGATAATTTTCCTGATGACATGTTGTAGAAGAGTCAATCGATCAGAACCTACGCAACACAATCTCAGAGGGACAGGGAGGGAGGTGTCAGTCACTCCCCAAAGCGGGAAGATCATATCTTCATGGGAATCACACAAGAGTGGGGGTGAGACCAGACTGTAAttaattaaCGTCCTTTCAACGATCCAAGTCcatgaaaaaaactaacacccctcccgtacctagcTTATAAAGTGCTGGGTCATCTAAGCTTTTCAGTCGAGAAAAAAACATTAGATCAGAAGAACAACTGGCAACACTTCTCAACCTGAGACTTACTTCAAGATGCTCGATCCTGGAGAGGTCTATGATGACCCTATTGACCCAATCGAGTTAGAGGCTGAACCCAGAGGAACCCCCATTGTCCCCAACATCTTGAGGAACTCTGACTACAATCTCAACTCTCCTTTGATAGAAGATCCTGCTAGACTAATGTTAGAATGGTTAAAAACAGGGAATAGACCTTATCGGATGACTCTAACAGACAATTGCTCCAGGTCTTTCAGAGTTTTGAAAGATTATTTCAAGAAGGTAGATTTGGGTTCTCTCAAGGTGGGCGGAATGGCTGCACAGTCAATGATTTCTCTCTGGTTATATGGTGCCCACTCTGAATCCAACAGGAGCCGGAGATGTATAACAGACTTGGCCCATTTCTATTCCAAGTCGTCCCCCATAGAGAAGCTGTTGAATCTCACGCTAGGAAATAGAGGGCTGAGAATCCCCCCAGAGGGAGTGTTAAGTTGCCTTGAGAGGGTTGATTATGATAATGCATTTGGAAGGTATCTTGCCAACACGTATTCCTCTTACTTGTTCTTCCATGTAATCACCTTATACATGAACGCCCTAGACTGGGATGAAGAAAAGACCATCCTAGCATTATGGAAAGATTTAACCTCAGTGGACATCGGGAAGGACTTGGTAAAGTTCAAAGACCAAATATGGGGACTGCTGATCGTGACAAAGGACTTTGTTTACTCCCAAAGTTCCAATTGTCTTTTTGACAGAAACTACACACTTATGCTAAAAGATCTTTTCTTGTCTCGCTTCAACTCCTTAATGGTCTTGCTCTCTCCCCCAGAGCCCCGATACTCAGATGACTTGATATCTCAACTATGCCAGCTGTACATTGCTGGGGATCAAGTCTTGTCTATGTGTGGAAACTCCGGCTATGAAGTCATCAAAATATTGGAGCCATATGTCGTGAATAGTTTAGTCCAGAGAGCAGAAAAGTTTAGGCCTCTCATTCATTCCTTGGGAGACTTTCCTGTATTTATAAAAGACAAGGTAAGTCAACTTGAAGAGACGTTCGGTCCCTGTGCAAGAAGGTTCTTTAGGGCTCTGGATCAATTCGACAACATACATGACTTGGTTTTTGTGTTTGGCTGTTACAGGCATTGGGGGCACCCATATATAGATTATCGAAAGGGTCTGTCAAAACTATATGATCAGGTTCACCTTAAAAAAATGATAGATAAGTCCTACCAGGAGTGCTTAGCAAGCGACCTAGCCAGGAGGATCCTTAGATGGGGTTTTGATAAGTACTCCAAGTGGTATCTGGATTCAAGATTCCTAGCCCGAGACCACCCCTTGACTCCTTATATCAAAACCCAAACATGGCCACCCAAACATATTGTAGACTTGGTGGGGGATACATGGCACAAGCTCCCGATCACGCAGATCTTTGAGATTCCTGAATCAATGGATCCGTCAGAAATATTGGATGACAAATCACATTCTTTCACCAGAACGAGACTAGCTTCTTGGCTGTCAGAAAACCGAGGGGGGCCTGTTCCTAGCGAAAAAGTTATTATCACGGCCCTGTCTAAGCCGCCTGTCAATCCCCGAGAGTTTCTGAGGTCTATAGACCTCGGAGGATTGCCAGATGAAGACTTGATAATTGGCCTCAAGCCAAAGGAACGGGAATTGAAGATTGAAGGTCGATTCTTTGCTCTAATGTCATGGAATCTAAGATTGTATTTTGTCATCACTGAAAAACTCTTGGCCAACTACATCTTGCCACTTTTTGACGCGCTGACTATGACAGACAACCTGAACAAGGTGTTTAAAAAGCTGATCGACAGGGTCACCGGGCAAGGGCTTTTGGACTATTCAAGGGTCACATATGCATTTCACCTGGACTATGAAAAGTGGAACAACCATCAAAGATTAGAGTCAACAGAGGATGTATTTTCTGTCCTAGATCAAGTGTTTGGATTGAAGAGAGTGTTTTCTAGAACACACGAGTTTTTTCAAAAGGCCTGGATCTATTATTCAGACAGATCAGACCTCATCGGGTTACGGGAGGATCAAATATACTGCTTAGATGCGTCCAACGGCCCAACCTGTTGGAATGGCCAGGATGGCGGGCTAGAAGGCTTACGGCAGAAGGGCTGGAGTCTAGTCAGCTTATTGATGATAGATAGAGAATCTCAAATCAGGAACACAAGAACCAAAATACTAGCTCAAGGAGACAACCAGGTTTTATGTCCGACATACATGTTGTCGCCAGGGCTATCTCAAGAGGGGCTCCTCTATGAATTGGAGAGAATATCAAGGAATGCACTTTCGATATACAGAGCCGTCGAGGAAGGGGCATCTAAGCTAGGGCTGATCATCAAGAAAGAAGAGACCATGTGTAGTTATGACTTCCTCATCTATGGAAAAACCCCTTTGTTTAGAGGTAACATATTGGTGCCTGAGTCCAAAAGATGGGCCAGAGTCTCTTGCGTCTCTAATGACCAAATAGTCAACCTCGCCAATATAATGTCGACAGTGTCCACCAATGCGCTAACAGTGGCACAACACTCTCAATCTTTGATCAAACCGATGAGGGATTTTCTGCTCATGTCAGTACAGGCAGTCTTTCACTACCTGCTATTTAGCCCAATCTTAAAGGGAAGAGTTTACAAGATTCTGAGCGCTGAAGGGGAGAGCTTTCTCCTAGCCATGTCAAGGATAATCTATCTAGATCCTTCTTTGGGAGGGATATCTGGAATGTCCCTCGGAAGATTCCATATACGACAGTTCTCAGACCCTGTCTCTGAAGGGTTATCCTTCTGGAGAGAGATCTGGTTAAGCTCCCAAGAGTCCTGGATTCACGCGTTGTGTCAAGAGGCTGGAAACCCAGATCTTGGAGAGAGAACACTCGAGAGCTTCACTCGCCTTCTAGAAGATCCGACCACCTTAAATATCAGAGGAGGGGCCAGTCCTACCATTCTACTCAAGGATGCAATCAGAAAGGCTTTATATGACGAGGTGGACAAGGTGGAAAATTCAGAGTTTCGAGAGGCAATCCTGTTGTCCAAGACCCATAGAGATAATTTTATACTCTTCTTAATATCTGTTGAGCCTCTGTTTCCTCGATTTCTCAGTGAGCTATTCAGTTCGTCTTTTTTGGGAATCCCCGAGTCAATCATTGGATTGATACAAAACTCCCGAACGATAAGAAGGCAGTTTAGAAAGAGTCTCTCAAAAACTTTAGAAGAATCCTTCTACAACTCAGAGATCCACGGGATTAGTCGGATGACCCAGACACCTCAGAGGGTTGGGGGGGTGTGGCCTTGCTCTTCAGAGAGGGCAGATCTACTTAGGGAGATCTCTTGGGGAAGAAAAGTGGTAGGCACGACAGTTCCTCACCCTTCTGAGATGTTGGGATTACTTCCCAAGTCCTCTATTTCTTGCACTTGTGGAGCAACAGGAGGAGGCAATCCTAGAGTTTCTGTATCAGTACTCCCGTCCTTTGATCAGTCATTTTTTTCACGAGGCCCCCTAAAGGGATACTTGGGCTCGTCCACCTCTATGTCGACCCAGCTATTCCATGCATGGGAAAAAGTCACTAATGTTCATGTGGTGAAGAGAGCTCTATCGTTAAAAGAATCTATAAACTGGTTCATTACTAGAGATTCCAACTTGGCTCAAGCTCTAATTAGGAACATTATGTCTCTGACAGGCCCTGATTTCCCTCTAGAGGAGGCCCCTGTCTTCAAAAGGACGGGGTCAGCCTTGCATAGGTTCAAGTCTGCCAGATACAGCGAAGGAGGGTATTCTTCTGTCTGCCCGAACCTCCTCTCTCATATTTCTGTTAGTACAGACACCATGTCTGATTTGACCCAAGACGGGAAGAACTACGATTTCATGTTCCAGCCATTGATGCTTTATGCACAGACATGGACATCAGAGCTGGTACAGAGAGACACAAGGCTAAGAGACTCTACGTTTCATTGGCACCTCCGATGCAACAGGTGTGTGAGACCCATTGACGACGTGACCCTGGAGACCTCTCAGATCTTCGAGTTTCCGGATGTGTCGAAAAGAATATCCAGAATGGTTTCTGGGGCTGTGCCTCACTTCCAGAGGCTTCCCGATATCCGTCTGAGACCAGGAGATTTTGAATCTCTAAGCGGTAGAGAAAAGTCTCACCATATCGGATCAGCTCAGGGGCTCTTATACTCAATCTTAGTGGCAATTCACGACTCAGGATACAATGATGGAACCATCTTCCCTGTCAACATATACGGCAAGGTTTCCCCTAGAGACTATTTGAGAGGGCTCGCAAGGGGAGTATTGATAGGATCCTCGATTTGCTTCTTGACAAGAATGACAAATATCAATATTAATAGACCTCTTGAATTGGTCTCAGGGGTAATCTCATATATTCTCCTGAGGCTAGATAACCATCCCTCCTTGTACATAATGCTCAGAGAACCGTCTCTTAGAGGAGAGATATTTTCTATCCCTCAGAAAATCCCCGCCGCTTATCCAACCACTATGAAAGAAGGCAACAGATCAATCTTGTGTTATCTCCAACATGTGCTACGCTATGAGCGAGAGATAATCACGGCGTCTCCAGAGAATGACTGGCTATGGATCTTTTCAGACTTTAGAAGTGCCAAAATGACGTACCTATCCCTCATTACTTACCAGTCTCATCTTCTACTCCAGAGGGTTGAGAGAAACCTATCTAAGAGTATGAGAGATAACCTGCGACAATTGAGTTCTTTGATGAGGCAGGTGCTGGGCGGGCACGGAGAAGATACCTTAGAGTCAGACGACAACATTCAACGACTGCTAAAAGACTCTTTACGAAGGACAAGATGGGTGGATCAAGAGGTGCGCCATGCAGCTAGAACCATGACTGGAGATTACAGCCCCAACAAGAAGGTGTCCCGTAAGGTAGGATGTTCAGAATGGGTCTGCTCTGCTCAACAGGTTGCAGTCTCTACCTCAGCAAACCCGGCCCCTGTCTCGGAGCTTGACATAAGGGCCCTCTCTAAGAGGTTCCAGAACCCTTTGATCTCGGGCTTGAGAGTGGTTCAGTGGGCAACCGGTGCTCATTATAAGCTTAAGCCTATTCTAGATGATCTCAATGTTTTCCCATCTCTCTGCCTTGTAGTTGGGGACGGGTCAGGGGGGATATCAAGGGCAGTCCTCAACATGTTTCCAGATGCCAAGCTTGTGTTCAACAGTCTTTTAGAGGTGAATGACCTGATGGCTTCCGGAACACATCCACTGCCTCCTTCAGCAATCATGAGGGGAGGAAATGATATCGTCTCCAGAGTGATAGATCTTGACTCAATCTGGGAAAAACCGTCCGACTTGAGAAACTTGGCAACCTGGAAATACTTCCAGTCAGTCCAAAAGCAGGTCAACATGTCCTATGACCTCATTATTTGCGATGCAGAAGTTACTGACATTGCATCTATCAACCGGATCACCCTGTTAATGTCCGATTTTGCATTGTCTATAGATGGACCACTCTATTTGGTCTTCAAAACTTATGGGACTATGCTAGTAAATCCAAACTACAAGGCTATTCAACACCTGTCAAGAGCGTTCCCCTCGGTCACAGGGTTTATCACCCAAGTAACTTCGTCTTTTTCATCTGAGCTCTACCTCCGATTCTCCAAACGAGGGAAGTTTTTCAGAGATGCTGAGTACTTGACCTCTTCCACCCTTCGAGAAATGAGCCTTGTGTTATTCAATTGTAGCAGCCCCAAGAGTGAGATGCAGAGAGCTCGTTCCTTGAACTATCAGGATCTTGTGAGAGGATTTCCTGAAGAAATCATATCAAATCCTTACAATGAGATGATCATAACTCTGATTGACAGTGATGTAGAATCTTTTCTAGTCCACAAGATGGTTGATGATCTTGAGTTACAGAGGGGAACTCTGTCTAAAGTGGCTATCATTATAGCCATCATGATAGTTTTCTCCAACAGAGTCTTCAACGTTTCCAAACCCCTAACTGACCCCTCGTTCTATCCACCGTCTGATCCCAAAATCCTGAGGCACTTCAACATATGTTGCAGTACTATGATGTATCTATCTACTGCTTTAGGTGACGTCCCTAGCTTCGCAAGACTTCACGACCTGTATAACAGACCTATAACTTATTACTTCAGAAAGCAAGTCATTCGAGGGAACGTTTATCTATCTTGGAGTTGGTCCAACGACACCTCAGTGTTCAAAAGGGTAGCCTGTAATTCTAGCCTGAGTCTGTCATCTCACTGGATCAGGTTGATTTACAAGATAGTGAAGACTACCAGACTCGTTGGCAGCATCAAGGATCTATCCAGAGAAGTGGAAAGACACCTTCATAGGTACAACAGGTGGATCACCCTAGAGGATATCAGATCTAGATCATCCCTACTAGACTACAGTTGCCTGTGAACCGGATACTCCTGGAAGCCTGCCCATGCTAAGACTCTTGTGTGATGTATCTTGAAAAAAACAAGATCCTAAATCTGAACCTTTGGTTGTTTGATTGTTTTTCTCAtttttgttgtttatttgttaagcgt
在一个实施方式中，核酸包括SEQ ID NO：14的序列，如下所示(RABV载体：CoravaxV5 China)：
ACGCTTAACAACCAGATCAAAGAAAAAACAGACATTGTCAATTGCAAAGCAAAAATGTAACACCCCTACAATGGATGCCGACAAGATTGTATTCAAAGTCAATAATCAGGTGGTCTCTTTGAAGCCTGAGATTATCGTGGATCAATATGAGTACAAGTACCCTGCCATCAAAGATTTGAAAAAGCCCTGTATAACCCTAGGAAAGGCTCCCGATTTAAATAAAGCATACAAGTCAGTTTTGTCAGGCATGAGCGCCGCCAAACTTAATCCTGACGATGTATGTTCCTATTTGGCAGCGGCAATGCAGTTTTTTGAGGGGACATGTCCGGAAGACTGGACCAGCTATGGAATTGTGATTGCACGAAAAGGAGATAAGATCACCCCAGGTTCTCTGGTGGAGATAAAACGTACTGATGTAGAAGGGAATTGGGCTCTGACAGGAGGCATGGAACTGACAAGAGACCCCACTGTCCCTGAGCATGCGTCCTTAGTCGGTCTTCTCTTGAGTCTGTATAGGTTGAGCAAAATATCCGGGCAAAACACTGGTAACTATAAGACAAACATTGCAGACAGGATAGAGCAGATTTTTGAGACAGCCCCTTTTGTTAAAATCGTGGAACACCATACTCTAATGACAACTCACAAAATGTGTGCTAATTGGAGTACTATACCAAACTTCAGATTTTTGGCCGGAACCTATGACATGTTTTTCTCCCGGATTGAGCATCTATATTCAGCAATCAGAGTGGGCACAGTTGTCACTGCTTATGAAGACTGTTCAGGACTGGTATCATTTACTGGGTTCATAAAACAAATCAATCTCACCGCTAGAGAGGCAATACTATATTTCTTCCACAAGAACTTTGAGGAAGAGATAAGAAGAATGTTTGAGCCAGGGCAGGAGACAGCTGTTCCTCACTCTTATTTCATCCACTTCCGTTCACTAGGCTTGAGTGGGAAATCTCCTTATTCATCAAATGCTGTTGGTCACGTGTTCAATCTCATTCACTTTGTAGGATGCTATATGGGTCAAGTCAGATCCCTAAATGCAACGGTTATTGCTGCATGTGCTCCTCATGAAATGTCTGTTCTAGGGGGCTATCTGGGAGAGGAATTCTTCGGGAAAGGGACATTTGAAAGAAGATTCTTCAGAGATGAGAAAGAACTTCAAGAATACGAGGCGGCTGAACTGACAAAGACTGACGTAGCACTGGCAGATGATGGAACTGTCAACTCTGACGACGAGGACTACTTTTCAGGTGAAACCAGAAGTCCGGAGGCTGTTTATACTCGAATCATGATGAATGGAGGTCGACTAAAGAGATCTCACATACGGAGATATGTCTCAGTCAGTTCCAATCATCAAGCCCGTCCAAACTCATTCGCCGAGTTTCTAAACAAGACATATTCGAGTGACTCATAAGAAGTTGAATAACAAAATGCCGGAAATCTACGGATTGTGTATATCCATCATGAAAAAAACTAACACCCCTCCTTTCGAACCATCCCAAACATGAGCAAGATCTTTGTCAATCCTAGTGCTATTAGAGCCGGTCTGGCCGATCTTGAGATGGCTGAAGAAACTGTTGATCTGATCAATAGAAATATCGAAGACAATCAGGCTCATCTCCAAGGGGAACCCATAGAGGTGGACAATCTCCCTGAGGATATGGGGCGACTTCACCTGGATGATGGAAAATCGCCCAACCATGGTGAGATAGCCAAGGTGGGAGAAGGCAAGTATCGAGAGGACTTTCAGATGGATGAAGGAGAGGATCCTAGCTTCCTGTTCCAGTCATACCTGGAAAATGTTGGAGTCCAAATAGTCAGACAAATGAGGTCAGGAGAGAGATTTCTCAAGATATGGTCACAGACCGTAGAAGAGATTATATCCTATGTCGCGGTCAACTTTCCCAACCCTCCAGGAAAGTCTTCAGAGGATAAATCAACCCAGACTACTGGCCGAGAGCTCAAGAAGGAGACAACACCCACTCCTTCTCAGAGAGAAAGCCAATCATCGAAAGCCAGGATGGCGGCTCAAATTGCTTCTGGCCCTCCAGCCCTTGAATGGTCGGCTACCAATGAAGAGGATGATCTATCAGTGGAGGCTGAGATCGCTCACCAGATTGCAGAAAGTTTCTCCAAAAAATATAAGTTTCCCTCTCGATCCTCAGGGATACTCTTGTATAATTTTGAGCAATTGAAAATGAACCTTGATGATATAGTTAAAGAGGCAAAAAATGTACCAGGTGTGACCCGTTTAGCCCATGACGGGTCCAAACTCCCCCTAAGATGTGTACTGGGATGGGTCGCTTTGGCCAACTCTAAGAAATTCCAGTTGTTAGTCGAATCCGACAAGCTGAGTAAAATCATGCAAGATGACTTGAATCGCTATACATCTTGCTAACCGAACCTCTCCCCTCAGTCCCTCTAGACAATAAAATCCGAGATGTCCCAAAGTCAACATGAAAAAAACAGGCAACACCACTGATAAAATGAACCTCCTACGTAAGATAGTGAAAAACCGCAGGGACGAGGACACTCAAAAATCCTCTCCCGCGTCAGCCCCTCTGGATGACGATGACTTGTGGCTTCCACCCCCTGAATACGTCCCGCTGAAAGAACTTACAGGCAAGAAGAACATGAGGAACTTTTGTATCAACGGAAGGGTTAAAGTGTGTAGCCCGAATGGTTACTCGTTCAGGATCCTGCGGCACATTCTGAAATCATTCGACGAGATATATTCTGGGAATCATAGGATGATCGGGTTAGTCAAAGTGGTTATTGGACTGGCTTTGTCAGGATCTCCAGTCCCTGAGGGCCTGAACTGGGTATACAAATTGAGGAGAACCTTTATCTTCCAGTGGGCTGATTCCAGGGGCCCTCTTGAAGGGGAGGAGTTGGAATACTCTCAGGAGATCACTTGGGATGATGATACTGAGTTCGTCGGATTGCAAATAAGAGTGATTGCAAAACAGTGTCATATCCAGGGCAGAGTCTGGTGTATCAACATGAACCCGAGAGCATGTCAACTATGGTCTGACATGTCTCTTCAGACACAAAGGTCCGAAGAGGACAAAGATTCCTCTCTGCTTCTAGAATAATCAGATTATATCCCGCAAATTTATCACTTGTTTACCTCTGGAGGAGAGAACATATGGGCTCAACTCCAACCCTTGGGAGCAATATAACAAAAAACATGTTATGGTGCCATTAAACCGCTGCATTTCATCAAAGTCAAGTTGATTACCTTTACATTTTGATCCTCTTGGATGTGAAAAAAACTATTAACATCCCTCAAAAGACCccgggccaccATGTTCGTGTTTCTGGTGCTGCTGCCTCTGGTGAGCTCCCAGTGCGTGAACCTGACCACAAGGACCCAGCTGCCCCCTGCCTATACCAATTCCTTCACACGGGGCGTGTACTATCCCGACAAGGTGTTCCGGAGCAGCGTGCTGCACTCCACACAGGATCTGTTTCTGCCTTTCTTTTCTAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAATGGCACAAAGCGGTTCGACAATCCAGTGCTGCCCTTTAACGATGGCGTGTACTTCGCCTCCACCGAGAAGTCTAACATCATCAGAGGCTGGATCTTTGGCACCACACTGGACAGCAAGACACAGTCCCTGCTGATCGTGAACAATGCCACCAACGTGGTCATCAAGGTGTGCGAGTTCCAGTTTTGTAATGATCCATTCCTGGGCGTGTACTATCACAAGAACAATAAGTCTTGGATGGAGAGCGAGTTTCGCGTGTATTCCTCTGCCAACAATTGCACATTTGAGTACGTGTCCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAATTTCAAGAACCTGAGGGAGTTCGTGTTTAAGAATATCGATGGCTACTTCAAAATCTACTCCAAGCACACCCCAATCAACCTGGTGCGCGACCTGCCACAGGGCTTCTCTGCCCTGGAGCCACTGGTGGATCTGCCCATCGGCATCAACATCACCCGGTTTCAGACACTGCTGGCCCTGCACAGAAGCTACCTGACACCAGGCGACAGCTCCTCTGGATGGACCGCAGGAGCAGCAGCCTACTATGTGGGCTATCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAATGGCACCATCACAGACGCCGTGGATTGCGCCCTGGATCCCCTGTCTGAGACCAAGTGTACACTGAAGAGCTTTACCGTGGAGAAGGGCATCTATCAGACAAGCAATTTCAGGGTGCAGCCTACCGAGTCCATCGTGCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAAGATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGGAgGGCTTTAACTGTTATTTCCCTCTGCAGAgCTACgGCTTCCAGCCAACAAACGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACcTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGACTGACCGGAACAGGCGTGCTGACCGAGTCCAACAAGAAGTTCCTGCCTTTTCAGCAGTTCGGCAGGGACATCGCAGATACCACAGACGCCGTGCGCGACCCTCAGACCCTGGAGATCCTGGACATCACACCATGCTCCTTCGGCGGCGTGTCTGTGATCACACCAGGCACCAATACAAGCAACCAGGTGGCCGTGCTGTATCAGGACGTGAATTGTACCGAGGTGCCAGTGGCAATCCACGCAGATCAGCTGACCCCTACATGGCGGGTGTACTCTACCGGCAGCAACGTGTTCCAGACAAGAGCCGGATGCCTGATCGGAGCAGAGCACGTGAACAATAGCTATGAGTGCGACATCCCTATCGGCGCCGGCATCTGTGCCTCCTACCAGACCCAGACAAACTCCCCAAGGTCTgtgggaGATGAGGCCGAAGACTTTGTGGAAGTCCACCTGCCTGATGTGCATAACCAGGTGTCTGGCGTCGACCTGGGACTGCCAAATTGGGGCAAGTACGTGCTGCTGAGTGCTGGAGCACTGACTGCCCTGATGCTGATCATTTTCCTGATGACCTGCTGTCGGCGCGTGAACAGAAGTGAGCCCACTCAGCACAATCTGCGAGGAACCGGGAGAGAAGTGTCAGTCACACCTCAGAGCGGGAAAATCATTAGTAGTTGGGAATCACATAAAAGCGGGGGCGAGACCAGGCTGggatccggctccggcgagggcaggggaagtctactaacatgcggggacgtggaggaaaatcccggccccATGGTTCCTCAGGCTCTCCTGTTTGTACCCCTTCTGGTTTTTCCATTGTGTTTTGGGAAATTCCCTATTTACACGATACCAGACAAGCTTGGTCCCTGGAGTCCGATTGACATACATCACCTCAGCTGCCCAAACAATTTGGTAGTGGAGGACGAAGGATGCACCAACCTGTCAGGGTTCTCCTACATGGAACTTAAAGTTGGATACATCTTAGCCATAAAAGTGAACGGGTTCACTTGCACAGGCGTTGTGACGGAGGCTGAAACCTACACTAACTTCGTTGGTTATGTCACAACCACGTTCAAAAGAAAGCATTTCCGCCCAACACCAGATGCATGTAGAGCCGCGTACAACTGGAAGATGGCCGGTGACCCCAGATATGAAGAGTCTCTACACAATCCGTACCCTGACTACCGCTGGCTTCGAACTGTAAAAACCACCAAGGAGTCTCTCGTTATCATATCTCCAAGTGTGGCAGATTTGGACCCATATGACAGATCCCTTCACTCGAGGGTCTTCCCTAGCGGGAAGTGCTCAGGAGTAGCGGTGTCTTCTACCTACTGCTCCACTAACCACGATTACACCATTTGGATGCCCGAGAATCCGAGACTAGGGATGTCTTGTGACATTTTTACCAATAGTAGAGGGAAGAGAGCATCCAAAGGGAGTGAGACTTGCGGCTTTGTAGATGAAAGAGGCCTATATAAGTCTTTAAAAGGAGCATGCAAACTCAAGTTATGTGGAGTTCTAGGACTTAGACTTATGGATGGAACATGGGTCTCGATGCAAACATCAAATGAAACCAAATGGTGCCCTCCCGATAAGTTGGTGAACCTGCACGACTTTCGCTCAGACGAAATTGAGCACCTTGTTGTAGAGGAGTTGGTCAGGAAGAGAGAGGAGTGTCTGGATGCACTAGAGTCCATCATGACAACCAAGTCAGTGAGTTTCAGACGTCTCAGTCATTTAAGAAAACTTGTCCCTGGGTTTGGAAAAGCATATACCATATTCAACAAGACCTTGATGGAAGCCGATGCTCACTACAAGTCAGTCgagACTTGGAATGAGATCCTCCCTTCAAAAGGGTGTTTAAGAGTTGGGGGGAGGTGTCATCCTCATGTGAACGGGGTGTTTTTCAATGGTATAATATTAGGACCTGACGGCAATGTCTTAATCCCAGAGATGCAATCATCCCTCCTCCAGCAACATATGGAGTTGTTGGAATCCTCGGTTATCCCCCTTGTGCACCCCCTGGCAGACCCGTCTACCGTTTTCAAGGACGGTGACGAGGCTGAGGATTTTGTTGAAGTTCACCTTCCCGATGTGCACAATCAGGTCTCAGGAGTTGACTTGGGTCTCCCGAACTGGGGGAAGTATGTATTACTGAGTGCAGGGGCCCTGACTGCCTTGATGTTGATAATTTTCCTGATGACATGTTGTAGAAGAGTCAATCGATCAGAACCTACGCAACACAATCTCAGAGGGACAGGGAGGGAGGTGTCAGTCACTCCCCAAAGCGGGAAGATCATATCTTCATGGGAATCACACAAGAGTGGGGGTGAGACCAGACTGTAAgctagcTTATAAAGTGCTGGGTCATCTAAGCTTTTCAGTCGAGAAAAAAACATTAGATCAGAAGAACAACTGGCAACACTTCTCAACCTGAGACTTACTTCAAGATGCTCGATCCTGGAGAGGTCTATGATGACCCTATTGACCCAATCGAGTTAGAGGCTGAACCCAGAGGAACCCCCATTGTCCCCAACATCTTGAGGAACTCTGACTACAATCTCAACTCTCCTTTGATAGAAGATCCTGCTAGACTAATGTTAGAATGGTTAAAAACAGGGAATAGACCTTATCGGATGACTCTAACAGACAATTGCTCCAGGTCTTTCAGAGTTTTGAAAGATTATTTCAAGAAGGTAGATTTGGGTTCTCTCAAGGTGGGCGGAATGGCTGCACAGTCAATGATTTCTCTCTGGTTATATGGTGCCCACTCTGAATCCAACAGGAGCCGGAGATGTATAACAGACTTGGCCCATTTCTATTCCAAGTCGTCCCCCATAGAGAAGCTGTTGAATCTCACGCTAGGAAATAGAGGGCTGAGAATCCCCCCAGAGGGAGTGTTAAGTTGCCTTGAGAGGGTTGATTATGATAATGCATTTGGAAGGTATCTTGCCAACACGTATTCCTCTTACTTGTTCTTCCATGTAATCACCTTATACATGAACGCCCTAGACTGGGATGAAGAAAAGACCATCCTAGCATTATGGAAAGATTTAACCTCAGTGGACATCGGGAAGGACTTGGTAAAGTTCAAAGACCAAATATGGGGACTGCTGATCGTGACAAAGGACTTTGTTTACTCCCAAAGTTCCAATTGTCTTTTTGACAGAAACTACACACTTATGCTAAAAGATCTTTTCTTGTCTCGCTTCAACTCCTTAATGGTCTTGCTCTCTCCCCCAGAGCCCCGATACTCAGATGACTTGATATCTCAACTATGCCAGCTGTACATTGCTGGGGATCAAGTCTTGTCTATGTGTGGAAACTCCGGCTATGAAGTCATCAAAATATTGGAGCCATATGTCGTGAATAGTTTAGTCCAGAGAGCAGAAAAGTTTAGGCCTCTCATTCATTCCTTGGGAGACTTTCCTGTATTTATAAAAGACAAGGTAAGTCAACTTGAAGAGACGTTCGGTCCCTGTGCAAGAAGGTTCTTTAGGGCTCTGGATCAATTCGACAACATACATGACTTGGTTTTTGTGTTTGGCTGTTACAGGCATTGGGGGCACCCATATATAGATTATCGAAAGGGTCTGTCAAAACTATATGATCAGGTTCACCTTAAAAAAATGATAGATAAGTCCTACCAGGAGTGCTTAGCAAGCGACCTAGCCAGGAGGATCCTTAGATGGGGTTTTGATAAGTACTCCAAGTGGTATCTGGATTCAAGATTCCTAGCCCGAGACCACCCCTTGACTCCTTATATCAAAACCCAAACATGGCCACCCAAACATATTGTAGACTTGGTGGGGGATACATGGCACAAGCTCCCGATCACGCAGATCTTTGAGATTCCTGAATCAATGGATCCGTCAGAAATATTGGATGACAAATCACATTCTTTCACCAGAACGAGACTAGCTTCTTGGCTGTCAGAAAACCGAGGGGGGCCTGTTCCTAGCGAAAAAGTTATTATCACGGCCCTGTCTAAGCCGCCTGTCAATCCCCGAGAGTTTCTGAGGTCTATAGACCTCGGAGGATTGCCAGATGAAGACTTGATAATTGGCCTCAAGCCAAAGGAACGGGAATTGAAGATTGAAGGTCGATTCTTTGCTCTAATGTCATGGAATCTAAGATTGTATTTTGTCATCACTGAAAAACTCTTGGCCAACTACATCTTGCCACTTTTTGACGCGCTGACTATGACAGACAACCTGAACAAGGTGTTTAAAAAGCTGATCGACAGGGTCACCGGGCAAGGGCTTTTGGACTATTCAAGGGTCACATATGCATTTCACCTGGACTATGAAAAGTGGAACAACCATCAAAGATTAGAGTCAACAGAGGATGTATTTTCTGTCCTAGATCAAGTGTTTGGATTGAAGAGAGTGTTTTCTAGAACACACGAGTTTTTTCAAAAGGCCTGGATCTATTATTCAGACAGATCAGACCTCATCGGGTTACGGGAGGATCAAATATACTGCTTAGATGCGTCCAACGGCCCAACCTGTTGGAATGGCCAGGATGGCGGGCTAGAAGGCTTACGGCAGAAGGGCTGGAGTCTAGTCAGCTTATTGATGATAGATAGAGAATCTCAAATCAGGAACACAAGAACCAAAATACTAGCTCAAGGAGACAACCAGGTTTTATGTCCGACATACATGTTGTCGCCAGGGCTATCTCAAGAGGGGCTCCTCTATGAATTGGAGAGAATATCAAGGAATGCACTTTCGATATACAGAGCCGTCGAGGAAGGGGCATCTAAGCTAGGGCTGATCATCAAGAAAGAAGAGACCATGTGTAGTTATGACTTCCTCATCTATGGAAAAACCCCTTTGTTTAGAGGTAACATATTGGTGCCTGAGTCCAAAAGATGGGCCAGAGTCTCTTGCGTCTCTAATGACCAAATAGTCAACCTCGCCAATATAATGTCGACAGTGTCCACCAATGCGCTAACAGTGGCACAACACTCTCAATCTTTGATCAAACCGATGAGGGATTTTCTGCTCATGTCAGTACAGGCAGTCTTTCACTACCTGCTATTTAGCCCAATCTTAAAGGGAAGAGTTTACAAGATTCTGAGCGCTGAAGGGGAGAGCTTTCTCCTAGCCATGTCAAGGATAATCTATCTAGATCCTTCTTTGGGAGGGATATCTGGAATGTCCCTCGGAAGATTCCATATACGACAGTTCTCAGACCCTGTCTCTGAAGGGTTATCCTTCTGGAGAGAGATCTGGTTAAGCTCCCAAGAGTCCTGGATTCACGCGTTGTGTCAAGAGGCTGGAAACCCAGATCTTGGAGAGAGAACACTCGAGAGCTTCACTCGCCTTCTAGAAGATCCGACCACCTTAAATATCAGAGGAGGGGCCAGTCCTACCATTCTACTCAAGGATGCAATCAGAAAGGCTTTATATGACGAGGTGGACAAGGTGGAAAATTCAGAGTTTCGAGAGGCAATCCTGTTGTCCAAGACCCATAGAGATAATTTTATACTCTTCTTAATATCTGTTGAGCCTCTGTTTCCTCGATTTCTCAGTGAGCTATTCAGTTCGTCTTTTTTGGGAATCCCCGAGTCAATCATTGGATTGATACAAAACTCCCGAACGATAAGAAGGCAGTTTAGAAAGAGTCTCTCAAAAACTTTAGAAGAATCCTTCTACAACTCAGAGATCCACGGGATTAGTCGGATGACCCAGACACCTCAGAGGGTTGGGGGGGTGTGGCCTTGCTCTTCAGAGAGGGCAGATCTACTTAGGGAGATCTCTTGGGGAAGAAAAGTGGTAGGCACGACAGTTCCTCACCCTTCTGAGATGTTGGGATTACTTCCCAAGTCCTCTATTTCTTGCACTTGTGGAGCAACAGGAGGAGGCAATCCTAGAGTTTCTGTATCAGTACTCCCGTCCTTTGATCAGTCATTTTTTTCACGAGGCCCCCTAAAGGGATACTTGGGCTCGTCCACCTCTATGTCGACCCAGCTATTCCATGCATGGGAAAAAGTCACTAATGTTCATGTGGTGAAGAGAGCTCTATCGTTAAAAGAATCTATAAACTGGTTCATTACTAGAGATTCCAACTTGGCTCAAGCTCTAATTAGGAACATTATGTCTCTGACAGGCCCTGATTTCCCTCTAGAGGAGGCCCCTGTCTTCAAAAGGACGGGGTCAGCCTTGCATAGGTTCAAGTCTGCCAGATACAGCGAAGGAGGGTATTCTTCTGTCTGCCCGAACCTCCTCTCTCATATTTCTGTTAGTACAGACACCATGTCTGATTTGACCCAAGACGGGAAGAACTACGATTTCATGTTCCAGCCATTGATGCTTTATGCACAGACATGGACATCAGAGCTGGTACAGAGAGACACAAGGCTAAGAGACTCTACGTTTCATTGGCACCTCCGATGCAACAGGTGTGTGAGACCCATTGACGACGTGACCCTGGAGACCTCTCAGATCTTCGAGTTTCCGGATGTGTCGAAAAGAATATCCAGAATGGTTTCTGGGGCTGTGCCTCACTTCCAGAGGCTTCCCGATATCCGTCTGAGACCAGGAGATTTTGAATCTCTAAGCGGTAGAGAAAAGTCTCACCATATCGGATCAGCTCAGGGGCTCTTATACTCAATCTTAGTGGCAATTCACGACTCAGGATACAATGATGGAACCATCTTCCCTGTCAACATATACGGCAAGGTTTCCCCTAGAGACTATTTGAGAGGGCTCGCAAGGGGAGTATTGATAGGATCCTCGATTTGCTTCTTGACAAGAATGACAAATATCAATATTAATAGACCTCTTGAATTGGTCTCAGGGGTAATCTCATATATTCTCCTGAGGCTAGATAACCATCCCTCCTTGTACATAATGCTCAGAGAACCGTCTCTTAGAGGAGAGATATTTTCTATCCCTCAGAAAATCCCCGCCGCTTATCCAACCACTATGAAAGAAGGCAACAGATCAATCTTGTGTTATCTCCAACATGTGCTACGCTATGAGCGAGAGATAATCACGGCGTCTCCAGAGAATGACTGGCTATGGATCTTTTCAGACTTTAGAAGTGCCAAAATGACGTACCTATCCCTCATTACTTACCAGTCTCATCTTCTACTCCAGAGGGTTGAGAGAAACCTATCTAAGAGTATGAGAGATAACCTGCGACAATTGAGTTCTTTGATGAGGCAGGTGCTGGGCGGGCACGGAGAAGATACCTTAGAGTCAGACGACAACATTCAACGACTGCTAAAAGACTCTTTACGAAGGACAAGATGGGTGGATCAAGAGGTGCGCCATGCAGCTAGAACCATGACTGGAGATTACAGCCCCAACAAGAAGGTGTCCCGTAAGGTAGGATGTTCAGAATGGGTCTGCTCTGCTCAACAGGTTGCAGTCTCTACCTCAGCAAACCCGGCCCCTGTCTCGGAGCTTGACATAAGGGCCCTCTCTAAGAGGTTCCAGAACCCTTTGATCTCGGGCTTGAGAGTGGTTCAGTGGGCAACCGGTGCTCATTATAAGCTTAAGCCTATTCTAGATGATCTCAATGTTTTCCCATCTCTCTGCCTTGTAGTTGGGGACGGGTCAGGGGGGATATCAAGGGCAGTCCTCAACATGTTTCCAGATGCCAAGCTTGTGTTCAACAGTCTTTTAGAGGTGAATGACCTGATGGCTTCCGGAACACATCCACTGCCTCCTTCAGCAATCATGAGGGGAGGAAATGATATCGTCTCCAGAGTGATAGATCTTGACTCAATCTGGGAAAAACCGTCCGACTTGAGAAACTTGGCAACCTGGAAATACTTCCAGTCAGTCCAAAAGCAGGTCAACATGTCCTATGACCTCATTATTTGCGATGCAGAAGTTACTGACATTGCATCTATCAACCGGATCACCCTGTTAATGTCCGATTTTGCATTGTCTATAGATGGACCACTCTATTTGGTCTTCAAAACTTATGGGACTATGCTAGTAAATCCAAACTACAAGGCTATTCAACACCTGTCAAGAGCGTTCCCCTCGGTCACAGGGTTTATCACCCAAGTAACTTCGTCTTTTTCATCTGAGCTCTACCTCCGATTCTCCAAACGAGGGAAGTTTTTCAGAGATGCTGAGTACTTGACCTCTTCCACCCTTCGAGAAATGAGCCTTGTGTTATTCAATTGTAGCAGCCCCAAGAGTGAGATGCAGAGAGCTCGTTCCTTGAACTATCAGGATCTTGTGAGAGGATTTCCTGAAGAAATCATATCAAATCCTTACAATGAGATGATCATAACTCTGATTGACAGTGATGTAGAATCTTTTCTAGTCCACAAGATGGTTGATGATCTTGAGTTACAGAGGGGAACTCTGTCTAAAGTGGCTATCATTATAGCCATCATGATAGTTTTCTCCAACAGAGTCTTCAACGTTTCCAAACCCCTAACTGACCCCTCGTTCTATCCACCGTCTGATCCCAAAATCCTGAGGCACTTCAACATATGTTGCAGTACTATGATGTATCTATCTACTGCTTTAGGTGACGTCCCTAGCTTCGCAAGACTTCACGACCTGTATAACAGACCTATAACTTATTACTTCAGAAAGCAAGTCATTCGAGGGAACGTTTATCTATCTTGGAGTTGGTCCAACGACACCTCAGTGTTCAAAAGGGTAGCCTGTAATTCTAGCCTGAGTCTGTCATCTCACTGGATCAGGTTGATTTACAAGATAGTGAAGACTACCAGACTCGTTGGCAGCATCAAGGATCTATCCAGAGAAGTGGAAAGACACCTTCATAGGTACAACAGGTGGATCACCCTAGAGGATATCAGATCTAGATCATCCCTACTAGACTACAGTTGCCTGTGAACCGGATACTCCTGGAAGCCTGCCCATGCTAAGACTCTTGTGTGATGTATCTTGAAAAAAACAAGATCCTAAATCTGAACCTTTGGTTGTTTGATTGTTTTTCTCATTTTTGTTGTTTATTTGTTAAGCGT。
在一个实施方式中，核酸包括SEQ ID NO：15的序列，如下所示(RABV载体：CoravaxV5 South Africa)：
ACGCTTAACAACCAGATCAAAGAAAAAACAGACATTGTCAATTGCAAAGCAAAAATGTAACACCCCTACAATGGATGCCGACAAGATTGTATTCAAAGTCAATAATCAGGTGGTCTCTTTGAAGCCTGAGATTATCGTGGATCAATATGAGTACAAGTACCCTGCCATCAAAGATTTGAAAAAGCCCTGTATAACCCTAGGAAAGGCTCCCGATTTAAATAAAGCATACAAGTCAGTTTTGTCAGGCATGAGCGCCGCCAAACTTAATCCTGACGATGTATGTTCCTATTTGGCAGCGGCAATGCAGTTTTTTGAGGGGACATGTCCGGAAGACTGGACCAGCTATGGAATTGTGATTGCACGAAAAGGAGATAAGATCACCCCAGGTTCTCTGGTGGAGATAAAACGTACTGATGTAGAAGGGAATTGGGCTCTGACAGGAGGCATGGAACTGACAAGAGACCCCACTGTCCCTGAGCATGCGTCCTTAGTCGGTCTTCTCTTGAGTCTGTATAGGTTGAGCAAAATATCCGGGCAAAACACTGGTAACTATAAGACAAACATTGCAGACAGGATAGAGCAGATTTTTGAGACAGCCCCTTTTGTTAAAATCGTGGAACACCATACTCTAATGACAACTCACAAAATGTGTGCTAATTGGAGTACTATACCAAACTTCAGATTTTTGGCCGGAACCTATGACATGTTTTTCTCCCGGATTGAGCATCTATATTCAGCAATCAGAGTGGGCACAGTTGTCACTGCTTATGAAGACTGTTCAGGACTGGTATCATTTACTGGGTTCATAAAACAAATCAATCTCACCGCTAGAGAGGCAATACTATATTTCTTCCACAAGAACTTTGAGGAAGAGATAAGAAGAATGTTTGAGCCAGGGCAGGAGACAGCTGTTCCTCACTCTTATTTCATCCACTTCCGTTCACTAGGCTTGAGTGGGAAATCTCCTTATTCATCAAATGCTGTTGGTCACGTGTTCAATCTCATTCACTTTGTAGGATGCTATATGGGTCAAGTCAGATCCCTAAATGCAACGGTTATTGCTGCATGTGCTCCTCATGAAATGTCTGTTCTAGGGGGCTATCTGGGAGAGGAATTCTTCGGGAAAGGGACATTTGAAAGAAGATTCTTCAGAGATGAGAAAGAACTTCAAGAATACGAGGCGGCTGAACTGACAAAGACTGACGTAGCACTGGCAGATGATGGAACTGTCAACTCTGACGACGAGGACTACTTTTCAGGTGAAACCAGAAGTCCGGAGGCTGTTTATACTCGAATCATGATGAATGGAGGTCGACTAAAGAGATCTCACATACGGAGATATGTCTCAGTCAGTTCCAATCATCAAGCCCGTCCAAACTCATTCGCCGAGTTTCTAAACAAGACATATTCGAGTGACTCATAAGAAGTTGAATAACAAAATGCCGGAAATCTACGGATTGTGTATATCCATCATGAAAAAAACTAACACCCCTCCTTTCGAACCATCCCAAACATGAGCAAGATCTTTGTCAATCCTAGTGCTATTAGAGCCGGTCTGGCCGATCTTGAGATGGCTGAAGAAACTGTTGATCTGATCAATAGAAATATCGAAGACAATCAGGCTCATCTCCAAGGGGAACCCATAGAGGTGGACAATCTCCCTGAGGATATGGGGCGACTTCACCTGGATGATGGAAAATCGCCCAACCATGGTGAGATAGCCAAGGTGGGAGAAGGCAAGTATCGAGAGGACTTTCAGATGGATGAAGGAGAGGATCCTAGCTTCCTGTTCCAGTCATACCTGGAAAATGTTGGAGTCCAAATAGTCAGACAAATGAGGTCAGGAGAGAGATTTCTCAAGATATGGTCACAGACCGTAGAAGAGATTATATCCTATGTCGCGGTCAACTTTCCCAACCCTCCAGGAAAGTCTTCAGAGGATAAATCAACCCAGACTACTGGCCGAGAGCTCAAGAAGGAGACAACACCCACTCCTTCTCAGAGAGAAAGCCAATCATCGAAAGCCAGGATGGCGGCTCAAATTGCTTCTGGCCCTCCAGCCCTTGAATGGTCGGCTACCAATGAAGAGGATGATCTATCAGTGGAGGCTGAGATCGCTCACCAGATTGCAGAAAGTTTCTCCAAAAAATATAAGTTTCCCTCTCGATCCTCAGGGATACTCTTGTATAATTTTGAGCAATTGAAAATGAACCTTGATGATATAGTTAAAGAGGCAAAAAATGTACCAGGTGTGACCCGTTTAGCCCATGACGGGTCCAAACTCCCCCTAAGATGTGTACTGGGATGGGTCGCTTTGGCCAACTCTAAGAAATTCCAGTTGTTAGTCGAATCCGACAAGCTGAGTAAAATCATGCAAGATGACTTGAATCGCTATACATCTTGCTAACCGAACCTCTCCCCTCAGTCCCTCTAGACAATAAAATCCGAGATGTCCCAAAGTCAACATGAAAAAAACAGGCAACACCACTGATAAAATGAACCTCCTACGTAAGATAGTGAAAAACCGCAGGGACGAGGACACTCAAAAATCCTCTCCCGCGTCAGCCCCTCTGGATGACGATGACTTGTGGCTTCCACCCCCTGAATACGTCCCGCTGAAAGAACTTACAGGCAAGAAGAACATGAGGAACTTTTGTATCAACGGAAGGGTTAAAGTGTGTAGCCCGAATGGTTACTCGTTCAGGATCCTGCGGCACATTCTGAAATCATTCGACGAGATATATTCTGGGAATCATAGGATGATCGGGTTAGTCAAAGTGGTTATTGGACTGGCTTTGTCAGGATCTCCAGTCCCTGAGGGCCTGAACTGGGTATACAAATTGAGGAGAACCTTTATCTTCCAGTGGGCTGATTCCAGGGGCCCTCTTGAAGGGGAGGAGTTGGAATACTCTCAGGAGATCACTTGGGATGATGATACTGAGTTCGTCGGATTGCAAATAAGAGTGATTGCAAAACAGTGTCATATCCAGGGCAGAGTCTGGTGTATCAACATGAACCCGAGAGCATGTCAACTATGGTCTGACATGTCTCTTCAGACACAAAGGTCCGAAGAGGACAAAGATTCCTCTCTGCTTCTAGAATAATCAGATTATATCCCGCAAATTTATCACTTGTTTACCTCTGGAGGAGAGAACATATGGGCTCAACTCCAACCCTTGGGAGCAATATAACAAAAAACATGTTATGGTGCCATTAAACCGCTGCATTTCATCAAAGTCAAGTTGATTACCTTTACATTTTGATCCTCTTGGATGTGAAAAAAACTATTAACATCCCTCAAAAGACCccgggccaccATGTTCGTGTTTCTGGTGCTGCTGCCTCTGGTGAGCTCCCAGTGCGTGAACTTCACCACAAGGACCCAGCTGCCCCCTGCCTATACCAATTCCTTCACACGGGGCGTGTACTATCCCGACAAGGTGTTCCGGAGCAGCGTGCTGCACTCCACACAGGATCTGTTTCTGCCTTTCTTTTCTAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAATGGCACAAAGCGGTTCGCCAATCCAGTGCTGCCCTTTAACGATGGCGTGTACTTCGCCTCCACCGAGAAGTCTAACATCATCAGAGGCTGGATCTTTGGCACCACACTGGACAGCAAGACACAGTCCCTGCTGATCGTGAACAATGCCACCAACGTGGTCATCAAGGTGTGCGAGTTCCAGTTTTGTAATGATCCATTCCTGGGCGTGTACTATCACAAGAACAATAAGTCTTGGATGGAGAGCGAGTTTCGCGTGTATTCCTCTGCCAACAATTGCACATTTGAGTACGTGTCCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAATTTCAAGAACCTGAGGGAGTTCGTGTTTAAGAATATCGATGGCTACTTCAAAATCTACTCCAAGCACACCCCAATCAACCTGGTGCGCGGCCTGCCACAGGGCTTCTCTGCCCTGGAGCCACTGGTGGATCTGCCCATCGGCATCAACATCACCCGGTTTCAGACACTGCTGGCCCTGCACAGAAGCTACCTGACACCAGGCGACAGCTCCTCTGGATGGACCGCAGGAGCAGCAGCCTACTATGTGGGCTATCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAATGGCACCATCACAGACGCCGTGGATTGCGCCCTGGATCCCCTGTCTGAGACCAAGTGTACACTGAAGAGCTTTACCGTGGAGAAGGGCATCTATCAGACAAGCAATTTCAGGGTGCAGCCTACCGAGTCCATCGTGCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAATATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGAAGGGCTTTAACTGTTATTTCCCTCTGCAGAGCTACGGCTTCCAGCCAACATATGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACCTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGACTGACCGGAACAGGCGTGCTGACCGAGTCCAACAAGAAGTTCCTGCCTTTTCAGCAGTTCGGCAGGGACATCGCAGATACCACAGACGCCGTGCGCGACCCTCAGACCCTGGAGATCCTGGACATCACACCATGCTCCTTCGGCGGCGTGTCTGTGATCACACCAGGCACCAATACAAGCAACCAGGTGGCCGTGCTGTATCAGGGCGTGAATTGTACCGAGGTGCCAGTGGCAATCCACGCAGATCAGCTGACCCCTACATGGCGGGTGTACTCTACCGGCAGCAACGTGTTCCAGACAAGAGCCGGATGCCTGATCGGAGCAGAGCACGTGAACAATAGCTATGAGTGCGACATCCCTATCGGCGCCGGCATCTGTGCCTCCTACCAGACCCAGACAAACTCCCCAGAATCAAGCGTGATTCCTCTGGTCCATCCACTGGCAGATCCCTCCACAGTGTTCAAAGACGGAGATGAGGCCGAAGACTTTGTGGAAGTCCACCTGCCTGATGTGCATAACCAGGTGTCTGGCGTCGACCTGGGACTGCCAAATTGGGGCAAGTACGTGCTGCTGAGTGCTGGAGCACTGACTGCCCTGATGCTGATCATTTTCCTGATGACCTGCTGTCGGCGCGTGAACAGAAGTGAGCCCACTCAGCACAATCTGCGAGGAACCGGGAGAGAAGTGTCAGTCACACCTCAGAGCGGGAAAATCATTAGTAGTTGGGAATCACATAAAAGCGGGGGCGAGACCAGGCTGggatccggctccggcgagggcaggggaagtctactaacatgcggggacgtggaggaaaatcccggccccATGGTTCCTCAGGCTCTCCTGTTTGTACCCCTTCTGGTTTTTCCATTGTGTTTTGGGAAATTCCCTATTTACACGATACCAGACAAGCTTGGTCCCTGGAGTCCGATTGACATACATCACCTCAGCTGCCCAAACAATTTGGTAGTGGAGGACGAAGGATGCACCAACCTGTCAGGGTTCTCCTACATGGAACTTAAAGTTGGATACATCTTAGCCATAAAAGTGAACGGGTTCACTTGCACAGGCGTTGTGACGGAGGCTGAAACCTACACTAACTTCGTTGGTTATGTCACAACCACGTTCAAAAGAAAGCATTTCCGCCCAACACCAGATGCATGTAGAGCCGCGTACAACTGGAAGATGGCCGGTGACCCCAGATATGAAGAGTCTCTACACAATCCGTACCCTGACTACCGCTGGCTTCGAACTGTAAAAACCACCAAGGAGTCTCTCGTTATCATATCTCCAAGTGTGGCAGATTTGGACCCATATGACAGATCCCTTCACTCGAGGGTCTTCCCTAGCGGGAAGTGCTCAGGAGTAGCGGTGTCTTCTACCTACTGCTCCACTAACCACGATTACACCATTTGGATGCCCGAGAATCCGAGACTAGGGATGTCTTGTGACATTTTTACCAATAGTAGAGGGAAGAGAGCATCCAAAGGGAGTGAGACTTGCGGCTTTGTAGATGAAAGAGGCCTATATAAGTCTTTAAAAGGAGCATGCAAACTCAAGTTATGTGGAGTTCTAGGACTTAGACTTATGGATGGAACATGGGTCTCGATGCAAACATCAAATGAAACCAAATGGTGCCCTCCCGATAAGTTGGTGAACCTGCACGACTTTCGCTCAGACGAAATTGAGCACCTTGTTGTAGAGGAGTTGGTCAGGAAGAGAGAGGAGTGTCTGGATGCACTAGAGTCCATCATGACAACCAAGTCAGTGAGTTTCAGACGTCTCAGTCATTTAAGAAAACTTGTCCCTGGGTTTGGAAAAGCATATACCATATTCAACAAGACCTTGATGGAAGCCGATGCTCACTACAAGTCAGTCgagACTTGGAATGAGATCCTCCCTTCAAAAGGGTGTTTAAGAGTTGGGGGGAGGTGTCATCCTCATGTGAACGGGGTGTTTTTCAATGGTATAATATTAGGACCTGACGGCAATGTCTTAATCCCAGAGATGCAATCATCCCTCCTCCAGCAACATATGGAGTTGTTGGAATCCTCGGTTATCCCCCTTGTGCACCCCCTGGCAGACCCGTCTACCGTTTTCAAGGACGGTGACGAGGCTGAGGATTTTGTTGAAGTTCACCTTCCCGATGTGCACAATCAGGTCTCAGGAGTTGACTTGGGTCTCCCGAACTGGGGGAAGTATGTATTACTGAGTGCAGGGGCCCTGACTGCCTTGATGTTGATAATTTTCCTGATGACATGTTGTAGAAGAGTCAATCGATCAGAACCTACGCAACACAATCTCAGAGGGACAGGGAGGGAGGTGTCAGTCACTCCCCAAAGCGGGAAGATCATATCTTCATGGGAATCACACAAGAGTGGGGGTGAGACCAGACTGTAAgctagcTTATAAAGTGCTGGGTCATCTAAGCTTTTCAGTCGAGAAAAAAACATTAGATCAGAAGAACAACTGGCAACACTTCTCAACCTGAGACTTACTTCAAGATGCTCGATCCTGGAGAGGTCTATGATGACCCTATTGACCCAATCGAGTTAGAGGCTGAACCCAGAGGAACCCCCATTGTCCCCAACATCTTGAGGAACTCTGACTACAATCTCAACTCTCCTTTGATAGAAGATCCTGCTAGACTAATGTTAGAATGGTTAAAAACAGGGAATAGACCTTATCGGATGACTCTAACAGACAATTGCTCCAGGTCTTTCAGAGTTTTGAAAGATTATTTCAAGAAGGTAGATTTGGGTTCTCTCAAGGTGGGCGGAATGGCTGCACAGTCAATGATTTCTCTCTGGTTATATGGTGCCCACTCTGAATCCAACAGGAGCCGGAGATGTATAACAGACTTGGCCCATTTCTATTCCAAGTCGTCCCCCATAGAGAAGCTGTTGAATCTCACGCTAGGAAATAGAGGGCTGAGAATCCCCCCAGAGGGAGTGTTAAGTTGCCTTGAGAGGGTTGATTATGATAATGCATTTGGAAGGTATCTTGCCAACACGTATTCCTCTTACTTGTTCTTCCATGTAATCACCTTATACATGAACGCCCTAGACTGGGATGAAGAAAAGACCATCCTAGCATTATGGAAAGATTTAACCTCAGTGGACATCGGGAAGGACTTGGTAAAGTTCAAAGACCAAATATGGGGACTGCTGATCGTGACAAAGGACTTTGTTTACTCCCAAAGTTCCAATTGTCTTTTTGACAGAAACTACACACTTATGCTAAAAGATCTTTTCTTGTCTCGCTTCAACTCCTTAATGGTCTTGCTCTCTCCCCCAGAGCCCCGATACTCAGATGACTTGATATCTCAACTATGCCAGCTGTACATTGCTGGGGATCAAGTCTTGTCTATGTGTGGAAACTCCGGCTATGAAGTCATCAAAATATTGGAGCCATATGTCGTGAATAGTTTAGTCCAGAGAGCAGAAAAGTTTAGGCCTCTCATTCATTCCTTGGGAGACTTTCCTGTATTTATAAAAGACAAGGTAAGTCAACTTGAAGAGACGTTCGGTCCCTGTGCAAGAAGGTTCTTTAGGGCTCTGGATCAATTCGACAACATACATGACTTGGTTTTTGTGTTTGGCTGTTACAGGCATTGGGGGCACCCATATATAGATTATCGAAAGGGTCTGTCAAAACTATATGATCAGGTTCACCTTAAAAAAATGATAGATAAGTCCTACCAGGAGTGCTTAGCAAGCGACCTAGCCAGGAGGATCCTTAGATGGGGTTTTGATAAGTACTCCAAGTGGTATCTGGATTCAAGATTCCTAGCCCGAGACCACCCCTTGACTCCTTATATCAAAACCCAAACATGGCCACCCAAACATATTGTAGACTTGGTGGGGGATACATGGCACAAGCTCCCGATCACGCAGATCTTTGAGATTCCTGAATCAATGGATCCGTCAGAAATATTGGATGACAAATCACATTCTTTCACCAGAACGAGACTAGCTTCTTGGCTGTCAGAAAACCGAGGGGGGCCTGTTCCTAGCGAAAAAGTTATTATCACGGCCCTGTCTAAGCCGCCTGTCAATCCCCGAGAGTTTCTGAGGTCTATAGACCTCGGAGGATTGCCAGATGAAGACTTGATAATTGGCCTCAAGCCAAAGGAACGGGAATTGAAGATTGAAGGTCGATTCTTTGCTCTAATGTCATGGAATCTAAGATTGTATTTTGTCATCACTGAAAAACTCTTGGCCAACTACATCTTGCCACTTTTTGACGCGCTGACTATGACAGACAACCTGAACAAGGTGTTTAAAAAGCTGATCGACAGGGTCACCGGGCAAGGGCTTTTGGACTATTCAAGGGTCACATATGCATTTCACCTGGACTATGAAAAGTGGAACAACCATCAAAGATTAGAGTCAACAGAGGATGTATTTTCTGTCCTAGATCAAGTGTTTGGATTGAAGAGAGTGTTTTCTAGAACACACGAGTTTTTTCAAAAGGCCTGGATCTATTATTCAGACAGATCAGACCTCATCGGGTTACGGGAGGATCAAATATACTGCTTAGATGCGTCCAACGGCCCAACCTGTTGGAATGGCCAGGATGGCGGGCTAGAAGGCTTACGGCAGAAGGGCTGGAGTCTAGTCAGCTTATTGATGATAGATAGAGAATCTCAAATCAGGAACACAAGAACCAAAATACTAGCTCAAGGAGACAACCAGGTTTTATGTCCGACATACATGTTGTCGCCAGGGCTATCTCAAGAGGGGCTCCTCTATGAATTGGAGAGAATATCAAGGAATGCACTTTCGATATACAGAGCCGTCGAGGAAGGGGCATCTAAGCTAGGGCTGATCATCAAGAAAGAAGAGACCATGTGTAGTTATGACTTCCTCATCTATGGAAAAACCCCTTTGTTTAGAGGTAACATATTGGTGCCTGAGTCCAAAAGATGGGCCAGAGTCTCTTGCGTCTCTAATGACCAAATAGTCAACCTCGCCAATATAATGTCGACAGTGTCCACCAATGCGCTAACAGTGGCACAACACTCTCAATCTTTGATCAAACCGATGAGGGATTTTCTGCTCATGTCAGTACAGGCAGTCTTTCACTACCTGCTATTTAGCCCAATCTTAAAGGGAAGAGTTTACAAGATTCTGAGCGCTGAAGGGGAGAGCTTTCTCCTAGCCATGTCAAGGATAATCTATCTAGATCCTTCTTTGGGAGGGATATCTGGAATGTCCCTCGGAAGATTCCATATACGACAGTTCTCAGACCCTGTCTCTGAAGGGTTATCCTTCTGGAGAGAGATCTGGTTAAGCTCCCAAGAGTCCTGGATTCACGCGTTGTGTCAAGAGGCTGGAAACCCAGATCTTGGAGAGAGAACACTCGAGAGCTTCACTCGCCTTCTAGAAGATCCGACCACCTTAAATATCAGAGGAGGGGCCAGTCCTACCATTCTACTCAAGGATGCAATCAGAAAGGCTTTATATGACGAGGTGGACAAGGTGGAAAATTCAGAGTTTCGAGAGGCAATCCTGTTGTCCAAGACCCATAGAGATAATTTTATACTCTTCTTAATATCTGTTGAGCCTCTGTTTCCTCGATTTCTCAGTGAGCTATTCAGTTCGTCTTTTTTGGGAATCCCCGAGTCAATCATTGGATTGATACAAAACTCCCGAACGATAAGAAGGCAGTTTAGAAAGAGTCTCTCAAAAACTTTAGAAGAATCCTTCTACAACTCAGAGATCCACGGGATTAGTCGGATGACCCAGACACCTCAGAGGGTTGGGGGGGTGTGGCCTTGCTCTTCAGAGAGGGCAGATCTACTTAGGGAGATCTCTTGGGGAAGAAAAGTGGTAGGCACGACAGTTCCTCACCCTTCTGAGATGTTGGGATTACTTCCCAAGTCCTCTATTTCTTGCACTTGTGGAGCAACAGGAGGAGGCAATCCTAGAGTTTCTGTATCAGTACTCCCGTCCTTTGATCAGTCATTTTTTTCACGAGGCCCCCTAAAGGGATACTTGGGCTCGTCCACCTCTATGTCGACCCAGCTATTCCATGCATGGGAAAAAGTCACTAATGTTCATGTGGTGAAGAGAGCTCTATCGTTAAAAGAATCTATAAACTGGTTCATTACTAGAGATTCCAACTTGGCTCAAGCTCTAATTAGGAACATTATGTCTCTGACAGGCCCTGATTTCCCTCTAGAGGAGGCCCCTGTCTTCAAAAGGACGGGGTCAGCCTTGCATAGGTTCAAGTCTGCCAGATACAGCGAAGGAGGGTATTCTTCTGTCTGCCCGAACCTCCTCTCTCATATTTCTGTTAGTACAGACACCATGTCTGATTTGACCCAAGACGGGAAGAACTACGATTTCATGTTCCAGCCATTGATGCTTTATGCACAGACATGGACATCAGAGCTGGTACAGAGAGACACAAGGCTAAGAGACTCTACGTTTCATTGGCACCTCCGATGCAACAGGTGTGTGAGACCCATTGACGACGTGACCCTGGAGACCTCTCAGATCTTCGAGTTTCCGGATGTGTCGAAAAGAATATCCAGAATGGTTTCTGGGGCTGTGCCTCACTTCCAGAGGCTTCCCGATATCCGTCTGAGACCAGGAGATTTTGAATCTCTAAGCGGTAGAGAAAAGTCTCACCATATCGGATCAGCTCAGGGGCTCTTATACTCAATCTTAGTGGCAATTCACGACTCAGGATACAATGATGGAACCATCTTCCCTGTCAACATATACGGCAAGGTTTCCCCTAGAGACTATTTGAGAGGGCTCGCAAGGGGAGTATTGATAGGATCCTCGATTTGCTTCTTGACAAGAATGACAAATATCAATATTAATAGACCTCTTGAATTGGTCTCAGGGGTAATCTCATATATTCTCCTGAGGCTAGATAACCATCCCTCCTTGTACATAATGCTCAGAGAACCGTCTCTTAGAGGAGAGATATTTTCTATCCCTCAGAAAATCCCCGCCGCTTATCCAACCACTATGAAAGAAGGCAACAGATCAATCTTGTGTTATCTCCAACATGTGCTACGCTATGAGCGAGAGATAATCACGGCGTCTCCAGAGAATGACTGGCTATGGATCTTTTCAGACTTTAGAAGTGCCAAAATGACGTACCTATCCCTCATTACTTACCAGTCTCATCTTCTACTCCAGAGGGTTGAGAGAAACCTATCTAAGAGTATGAGAGATAACCTGCGACAATTGAGTTCTTTGATGAGGCAGGTGCTGGGCGGGCACGGAGAAGATACCTTAGAGTCAGACGACAACATTCAACGACTGCTAAAAGACTCTTTACGAAGGACAAGATGGGTGGATCAAGAGGTGCGCCATGCAGCTAGAACCATGACTGGAGATTACAGCCCCAACAAGAAGGTGTCCCGTAAGGTAGGATGTTCAGAATGGGTCTGCTCTGCTCAACAGGTTGCAGTCTCTACCTCAGCAAACCCGGCCCCTGTCTCGGAGCTTGACATAAGGGCCCTCTCTAAGAGGTTCCAGAACCCTTTGATCTCGGGCTTGAGAGTGGTTCAGTGGGCAACCGGTGCTCATTATAAGCTTAAGCCTATTCTAGATGATCTCAATGTTTTCCCATCTCTCTGCCTTGTAGTTGGGGACGGGTCAGGGGGGATATCAAGGGCAGTCCTCAACATGTTTCCAGATGCCAAGCTTGTGTTCAACAGTCTTTTAGAGGTGAATGACCTGATGGCTTCCGGAACACATCCACTGCCTCCTTCAGCAATCATGAGGGGAGGAAATGATATCGTCTCCAGAGTGATAGATCTTGACTCAATCTGGGAAAAACCGTCCGACTTGAGAAACTTGGCAACCTGGAAATACTTCCAGTCAGTCCAAAAGCAGGTCAACATGTCCTATGACCTCATTATTTGCGATGCAGAAGTTACTGACATTGCATCTATCAACCGGATCACCCTGTTAATGTCCGATTTTGCATTGTCTATAGATGGACCACTCTATTTGGTCTTCAAAACTTATGGGACTATGCTAGTAAATCCAAACTACAAGGCTATTCAACACCTGTCAAGAGCGTTCCCCTCGGTCACAGGGTTTATCACCCAAGTAACTTCGTCTTTTTCATCTGAGCTCTACCTCCGATTCTCCAAACGAGGGAAGTTTTTCAGAGATGCTGAGTACTTGACCTCTTCCACCCTTCGAGAAATGAGCCTTGTGTTATTCAATTGTAGCAGCCCCAAGAGTGAGATGCAGAGAGCTCGTTCCTTGAACTATCAGGATCTTGTGAGAGGATTTCCTGAAGAAATCATATCAAATCCTTACAATGAGATGATCATAACTCTGATTGACAGTGATGTAGAATCTTTTCTAGTCCACAAGATGGTTGATGATCTTGAGTTACAGAGGGGAACTCTGTCTAAAGTGGCTATCATTATAGCCATCATGATAGTTTTCTCCAACAGAGTCTTCAACGTTTCCAAACCCCTAACTGACCCCTCGTTCTATCCACCGTCTGATCCCAAAATCCTGAGGCACTTCAACATATGTTGCAGTACTATGATGTATCTATCTACTGCTTTAGGTGACGTCCCTAGCTTCGCAAGACTTCACGACCTGTATAACAGACCTATAACTTATTACTTCAGAAAGCAAGTCATTCGAGGGAACGTTTATCTATCTTGGAGTTGGTCCAACGACACCTCAGTGTTCAAAAGGGTAGCCTGTAATTCTAGCCTGAGTCTGTCATCTCACTGGATCAGGTTGATTTACAAGATAGTGAAGACTACCAGACTCGTTGGCAGCATCAAGGATCTATCCAGAGAAGTGGAAAGACACCTTCATAGGTACAACAGGTGGATCACCCTAGAGGATATCAGATCTAGATCATCCCTACTAGACTACAGTTGCCTGTGAACCGGATACTCCTGGAAGCCTGCCCATGCTAAGACTCTTGTGTGATGTATCTTGAAAAAAACAAGATCCTAAATCTGAACCTTTGGTTGTTTGATTGTTTTTCTCATTTTTGTTGTTTATTTGTTAAGCGT。
在一个实施方式中，核酸包括SEQ ID NO：16的序列，如下所示(RABV载体：CoravaxV6 China)：
ACGCTTAACAACCAGATCAAAGAAAAAACAGACATTGTCAATTGCAAAGCAAAAATGTAACACCCCTACAATGGATGCCGACAAGATTGTATTCAAAGTCAATAATCAGGTGGTCTCTTTGAAGCCTGAGATTATCGTGGATCAATATGAGTACAAGTACCCTGCCATCAAAGATTTGAAAAAGCCCTGTATAACCCTAGGAAAGGCTCCCGATTTAAATAAAGCATACAAGTCAGTTTTGTCAGGCATGAGCGCCGCCAAACTTAATCCTGACGATGTATGTTCCTATTTGGCAGCGGCAATGCAGTTTTTTGAGGGGACATGTCCGGAAGACTGGACCAGCTATGGAATTGTGATTGCACGAAAAGGAGATAAGATCACCCCAGGTTCTCTGGTGGAGATAAAACGTACTGATGTAGAAGGGAATTGGGCTCTGACAGGAGGCATGGAACTGACAAGAGACCCCACTGTCCCTGAGCATGCGTCCTTAGTCGGTCTTCTCTTGAGTCTGTATAGGTTGAGCAAAATATCCGGGCAAAACACTGGTAACTATAAGACAAACATTGCAGACAGGATAGAGCAGATTTTTGAGACAGCCCCTTTTGTTAAAATCGTGGAACACCATACTCTAATGACAACTCACAAAATGTGTGCTAATTGGAGTACTATACCAAACTTCAGATTTTTGGCCGGAACCTATGACATGTTTTTCTCCCGGATTGAGCATCTATATTCAGCAATCAGAGTGGGCACAGTTGTCACTGCTTATGAAGACTGTTCAGGACTGGTATCATTTACTGGGTTCATAAAACAAATCAATCTCACCGCTAGAGAGGCAATACTATATTTCTTCCACAAGAACTTTGAGGAAGAGATAAGAAGAATGTTTGAGCCAGGGCAGGAGACAGCTGTTCCTCACTCTTATTTCATCCACTTCCGTTCACTAGGCTTGAGTGGGAAATCTCCTTATTCATCAAATGCTGTTGGTCACGTGTTCAATCTCATTCACTTTGTAGGATGCTATATGGGTCAAGTCAGATCCCTAAATGCAACGGTTATTGCTGCATGTGCTCCTCATGAAATGTCTGTTCTAGGGGGCTATCTGGGAGAGGAATTCTTCGGGAAAGGGACATTTGAAAGAAGATTCTTCAGAGATGAGAAAGAACTTCAAGAATACGAGGCGGCTGAACTGACAAAGACTGACGTAGCACTGGCAGATGATGGAACTGTCAACTCTGACGACGAGGACTACTTTTCAGGTGAAACCAGAAGTCCGGAGGCTGTTTATACTCGAATCATGATGAATGGAGGTCGACTAAAGAGATCTCACATACGGAGATATGTCTCAGTCAGTTCCAATCATCAAGCCCGTCCAAACTCATTCGCCGAGTTTCTAAACAAGACATATTCGAGTGACTCATAAGAAGTTGAATAACAAAATGCCGGAAATCTACGGATTGTGTATATCCATCATGAAAAAAACTAACACCCCTCCTTTCGAACCATCCCAAACATGAGCAAGATCTTTGTCAATCCTAGTGCTATTAGAGCCGGTCTGGCCGATCTTGAGATGGCTGAAGAAACTGTTGATCTGATCAATAGAAATATCGAAGACAATCAGGCTCATCTCCAAGGGGAACCCATAGAGGTGGACAATCTCCCTGAGGATATGGGGCGACTTCACCTGGATGATGGAAAATCGCCCAACCATGGTGAGATAGCCAAGGTGGGAGAAGGCAAGTATCGAGAGGACTTTCAGATGGATGAAGGAGAGGATCCTAGCTTCCTGTTCCAGTCATACCTGGAAAATGTTGGAGTCCAAATAGTCAGACAAATGAGGTCAGGAGAGAGATTTCTCAAGATATGGTCACAGACCGTAGAAGAGATTATATCCTATGTCGCGGTCAACTTTCCCAACCCTCCAGGAAAGTCTTCAGAGGATAAATCAACCCAGACTACTGGCCGAGAGCTCAAGAAGGAGACAACACCCACTCCTTCTCAGAGAGAAAGCCAATCATCGAAAGCCAGGATGGCGGCTCAAATTGCTTCTGGCCCTCCAGCCCTTGAATGGTCGGCTACCAATGAAGAGGATGATCTATCAGTGGAGGCTGAGATCGCTCACCAGATTGCAGAAAGTTTCTCCAAAAAATATAAGTTTCCCTCTCGATCCTCAGGGATACTCTTGTATAATTTTGAGCAATTGAAAATGAACCTTGATGATATAGTTAAAGAGGCAAAAAATGTACCAGGTGTGACCCGTTTAGCCCATGACGGGTCCAAACTCCCCCTAAGATGTGTACTGGGATGGGTCGCTTTGGCCAACTCTAAGAAATTCCAGTTGTTAGTCGAATCCGACAAGCTGAGTAAAATCATGCAAGATGACTTGAATCGCTATACATCTTGCTAACCGAACCTCTCCCCTCAGTCCCTCTAGACAATAAAATCCGAGATGTCCCAAAGTCAACATGAAAAAAACAGGCAACACCACTGATAAAATGAACCTCCTACGTAAGATAGTGAAAAACCGCAGGGACGAGGACACTCAAAAATCCTCTCCCGCGTCAGCCCCTCTGGATGACGATGACTTGTGGCTTCCACCCCCTGAATACGTCCCGCTGAAAGAACTTACAGGCAAGAAGAACATGAGGAACTTTTGTATCAACGGAAGGGTTAAAGTGTGTAGCCCGAATGGTTACTCGTTCAGGATCCTGCGGCACATTCTGAAATCATTCGACGAGATATATTCTGGGAATCATAGGATGATCGGGTTAGTCAAAGTGGTTATTGGACTGGCTTTGTCAGGATCTCCAGTCCCTGAGGGCCTGAACTGGGTATACAAATTGAGGAGAACCTTTATCTTCCAGTGGGCTGATTCCAGGGGCCCTCTTGAAGGGGAGGAGTTGGAATACTCTCAGGAGATCACTTGGGATGATGATACTGAGTTCGTCGGATTGCAAATAAGAGTGATTGCAAAACAGTGTCATATCCAGGGCAGAGTCTGGTGTATCAACATGAACCCGAGAGCATGTCAACTATGGTCTGACATGTCTCTTCAGACACAAAGGTCCGAAGAGGACAAAGATTCCTCTCTGCTTCTAGAATAATCAGATTATATCCCGCAAATTTATCACTTGTTTACCTCTGGAGGAGAGAACATATGGGCTCAACTCCAACCCTTGGGAGCAATATAACAAAAAACATGTTATGGTGCCATTAAACCGCTGCATTTCATCAAAGTCAAGTTGATTACCTTTACATTTTGATCCTCTTGGATGTGAAAAAAACTATTAACATCCCTCAAAAGACCccgggAAAGATGGTTCCTCAGGCTCTCCTGTTTGTACCCCTTCTGGTTTTTCCATTGTGTTTTGGGAAATTCCCTATTTACACGATACCAGACAAGCTTGGTCCCTGGAGTCCGATTGACATACATCACCTCAGCTGCCCAAACAATTTGGTAGTGGAGGACGAAGGATGCACCAACCTGTCAGGGTTCTCCTACATGGAACTTAAAGTTGGATACATCTTAGCCATAAAAGTGAACGGGTTCACTTGCACAGGCGTTGTGACGGAGGCTGAAACCTACACTAACTTCGTTGGTTATGTCACAACCACGTTCAAAAGAAAGCATTTCCGCCCAACACCAGATGCATGTAGAGCCGCGTACAACTGGAAGATGGCCGGTGACCCCAGATATGAAGAGTCTCTACACAATCCGTACCCTGACTACCGCTGGCTTCGAACTGTAAAAACCACCAAGGAGTCTCTCGTTATCATATCTCCAAGTGTGGCAGATTTGGACCCATATGACAGATCCCTTCACTCGAGGGTCTTCCCTAGCGGGAAGTGCTCAGGAGTAGCGGTGTCTTCTACCTACTGCTCCACTAACCACGATTACACCATTTGGATGCCCGAGAATCCGAGACTAGGGATGTCTTGTGACATTTTTACCAATAGTAGAGGGAAGAGAGCATCCAAAGGGAGTGAGACTTGCGGCTTTGTAGATGAAAGAGGCCTATATAAGTCTTTAAAAGGAGCATGCAAACTCAAGTTATGTGGAGTTCTAGGACTTAGACTTATGGATGGAACATGGGTCTCGATGCAAACATCAAATGAAACCAAATGGTGCCCTCCCGATAAGTTGGTGAACCTGCACGACTTTCGCTCAGACGAAATTGAGCACCTTGTTGTAGAGGAGTTGGTCAGGAAGAGAGAGGAGTGTCTGGATGCACTAGAGTCCATCATGACAACCAAGTCAGTGAGTTTCAGACGTCTCAGTCATTTAAGAAAACTTGTCCCTGGGTTTGGAAAAGCATATACCATATTCAACAAGACCTTGATGGAAGCCGATGCTCACTACAAGTCAGTCgagACTTGGAATGAGATCCTCCCTTCAAAAGGGTGTTTAAGAGTTGGGGGGAGGTGTCATCCTCATGTGAACGGGGTGTTTTTCAATGGTATAATATTAGGACCTGACGGCAATGTCTTAATCCCAGAGATGCAATCATCCCTCCTCCAGCAACATATGGAGTTGTTGGAATCCTCGGTTATCCCCCTTGTGCACCCCCTGGCAGACCCGTCTACCGTTTTCAAGGACGGTGACGAGGCTGAGGATTTTGTTGAAGTTCACCTTCCCGATGTGCACAATCAGGTCTCAGGAGTTGACTTGGGTCTCCCGAACTGGGGGAAGTATGTATTACTGAGTGCAGGGGCCCTGACTGCCTTGATGTTGATAATTTTCCTGATGACATGTTGTAGAAGAGTCAATCGATCAGAACCTACGCAACACAATCTCAGAGGGACAGGGAGGGAGGTGTCAGTCACTCCCCAAAGCGGGAAGATCATATCTTCATGGGAATCACACAAGAGTGGGGGTGAGACCAGACTGTAAttaattaaCGTCCTTTCAACGATCCAAGTCcatgaaaaaaactaacacccctcccgtacgaccATGTTCGTGTTTCTGGTGCTGCTGCCTCTGGTGAGCTCCCAGTGCGTGAACCTGACCACAAGGACCCAGCTGCCCCCTGCCTATACCAATTCCTTCACACGGGGCGTGTACTATCCCGACAAGGTGTTCCGGAGCAGCGTGCTGCACTCCACACAGGATCTGTTTCTGCCTTTCTTTTCTAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAATGGCACAAAGCGGTTCGACAATCCAGTGCTGCCCTTTAACGATGGCGTGTACTTCGCCTCCACCGAGAAGTCTAACATCATCAGAGGCTGGATCTTTGGCACCACACTGGACAGCAAGACACAGTCCCTGCTGATCGTGAACAATGCCACCAACGTGGTCATCAAGGTGTGCGAGTTCCAGTTTTGTAATGATCCATTCCTGGGCGTGTACTATCACAAGAACAATAAGTCTTGGATGGAGAGCGAGTTTCGCGTGTATTCCTCTGCCAACAATTGCACATTTGAGTACGTGTCCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAATTTCAAGAACCTGAGGGAGTTCGTGTTTAAGAATATCGATGGCTACTTCAAAATCTACTCCAAGCACACCCCAATCAACCTGGTGCGCGACCTGCCACAGGGCTTCTCTGCCCTGGAGCCACTGGTGGATCTGCCCATCGGCATCAACATCACCCGGTTTCAGACACTGCTGGCCCTGCACAGAAGCTACCTGACACCAGGCGACAGCTCCTCTGGATGGACCGCAGGAGCAGCAGCCTACTATGTGGGCTATCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAATGGCACCATCACAGACGCCGTGGATTGCGCCCTGGATCCCCTGTCTGAGACCAAGTGTACACTGAAGAGCTTTACCGTGGAGAAGGGCATCTATCAGACAAGCAATTTCAGGGTGCAGCCTACCGAGTCCATCGTGCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAAGATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGGAgGGCTTTAACTGTTATTTCCCTCTGCAGAgCTACgGCTTCCAGCCAACAAACGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACcTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGACTGACCGGAACAGGCGTGCTGACCGAGTCCAACAAGAAGTTCCTGCCTTTTCAGCAGTTCGGCAGGGACATCGCAGATACCACAGACGCCGTGCGCGACCCTCAGACCCTGGAGATCCTGGACATCACACCATGCTCCTTCGGCGGCGTGTCTGTGATCACACCAGGCACCAATACAAGCAACCAGGTGGCCGTGCTGTATCAGGACGTGAATTGTACCGAGGTGCCAGTGGCAATCCACGCAGATCAGCTGACCCCTACATGGCGGGTGTACTCTACCGGCAGCAACGTGTTCCAGACAAGAGCCGGATGCCTGATCGGAGCAGAGCACGTGAACAATAGCTATGAGTGCGACATCCCTATCGGCGCCGGCATCTGTGCCTCCTACCAGACCCAGACAAACTCCCCAAGGTCTGTGGGAGATGAGGCCGAAGACTTTGTGGAAGTCCACCTGCCTGATGTGCATAACCAGGTGTCTGGCGTCGACCTGGGACTGCCAAATTGGGGCAAGTACGTGCTGCTGAGTGCTGGAGCACTGACTGCCCTGATGCTGATCATTTTCCTGATGACCTGCTGTCGGCGCGTGAACAGAAGTGAGCCCACTCAGCACAATCTGCGAGGAACCGGGAGAGAAGTGTCAGTCACACCTCAGAGCGGGAAAATCATTAGTAGTTGGGAATCACATAAAAGCGGGGGCGAGACCAGGCTGggatccggctccggcgagggcaggggaagtctactaacatgcggggacgtggaggaaaatcccggccccATGCTCGATCCTGGAGAGGTCTATGATGACCCTATTGACCCAATCGAGTTAGAGGCTGAACCCAGAGGAACCCCCATTGTCCCCAACATCTTGAGGAACTCTGACTACAATCTCAACTCTCCTTTGATAGAAGATCCTGCTAGACTAATGTTAGAATGGTTAAAAACAGGGAATAGACCTTATCGGATGACTCTAACAGACAATTGCTCCAGGTCTTTCAGAGTTTTGAAAGATTATTTCAAGAAGGTAGATTTGGGTTCTCTCAAGGTGGGCGGAATGGCTGCACAGTCAATGATTTCTCTCTGGTTATATGGTGCCCACTCTGAATCCAACAGGAGCCGGAGATGTATAACAGACTTGGCCCATTTCTATTCCAAGTCGTCCCCCATAGAGAAGCTGTTGAATCTCACGCTAGGAAATAGAGGGCTGAGAATCCCCCCAGAGGGAGTGTTAAGTTGCCTTGAGAGGGTTGATTATGATAATGCATTTGGAAGGTATCTTGCCAACACGTATTCCTCTTACTTGTTCTTCCATGTAATCACCTTATACATGAACGCCCTAGACTGGGATGAAGAAAAGACCATCCTAGCATTATGGAAAGATTTAACCTCAGTGGACATCGGGAAGGACTTGGTAAAGTTCAAAGACCAAATATGGGGACTGCTGATCGTGACAAAGGACTTTGTTTACTCCCAAAGTTCCAATTGTCTTTTTGACAGAAACTACACACTTATGCTAAAAGATCTTTTCTTGTCTCGCTTCAACTCCTTAATGGTCTTGCTCTCTCCCCCAGAGCCCCGATACTCAGATGACTTGATATCTCAACTATGCCAGCTGTACATTGCTGGGGATCAAGTCTTGTCTATGTGTGGAAACTCCGGCTATGAAGTCATCAAAATATTGGAGCCATATGTCGTGAATAGTTTAGTCCAGAGAGCAGAAAAGTTTAGGCCTCTCATTCATTCCTTGGGAGACTTTCCTGTATTTATAAAAGACAAGGTAAGTCAACTTGAAGAGACGTTCGGTCCCTGTGCAAGAAGGTTCTTTAGGGCTCTGGATCAATTCGACAACATACATGACTTGGTTTTTGTGTTTGGCTGTTACAGGCATTGGGGGCACCCATATATAGATTATCGAAAGGGTCTGTCAAAACTATATGATCAGGTTCACCTTAAAAAAATGATAGATAAGTCCTACCAGGAGTGCTTAGCAAGCGACCTAGCCAGGAGGATCCTTAGATGGGGTTTTGATAAGTACTCCAAGTGGTATCTGGATTCAAGATTCCTAGCCCGAGACCACCCCTTGACTCCTTATATCAAAACCCAAACATGGCCACCCAAACATATTGTAGACTTGGTGGGGGATACATGGCACAAGCTCCCGATCACGCAGATCTTTGAGATTCCTGAATCAATGGATCCGTCAGAAATATTGGATGACAAATCACATTCTTTCACCAGAACGAGACTAGCTTCTTGGCTGTCAGAAAACCGAGGGGGGCCTGTTCCTAGCGAAAAAGTTATTATCACGGCCCTGTCTAAGCCGCCTGTCAATCCCCGAGAGTTTCTGAGGTCTATAGACCTCGGAGGATTGCCAGATGAAGACTTGATAATTGGCCTCAAGCCAAAGGAACGGGAATTGAAGATTGAAGGTCGATTCTTTGCTCTAATGTCATGGAATCTAAGATTGTATTTTGTCATCACTGAAAAACTCTTGGCCAACTACATCTTGCCACTTTTTGACGCGCTGACTATGACAGACAACCTGAACAAGGTGTTTAAAAAGCTGATCGACAGGGTCACCGGGCAAGGGCTTTTGGACTATTCAAGGGTCACATATGCATTTCACCTGGACTATGAAAAGTGGAACAACCATCAAAGATTAGAGTCAACAGAGGATGTATTTTCTGTCCTAGATCAAGTGTTTGGATTGAAGAGAGTGTTTTCTAGAACACACGAGTTTTTTCAAAAGGCCTGGATCTATTATTCAGACAGATCAGACCTCATCGGGTTACGGGAGGATCAAATATACTGCTTAGATGCGTCCAACGGCCCAACCTGTTGGAATGGCCAGGATGGCGGGCTAGAAGGCTTACGGCAGAAGGGCTGGAGTCTAGTCAGCTTATTGATGATAGATAGAGAATCTCAAATCAGGAACACAAGAACCAAAATACTAGCTCAAGGAGACAACCAGGTTTTATGTCCGACATACATGTTGTCGCCAGGGCTATCTCAAGAGGGGCTCCTCTATGAATTGGAGAGAATATCAAGGAATGCACTTTCGATATACAGAGCCGTCGAGGAAGGGGCATCTAAGCTAGGGCTGATCATCAAGAAAGAAGAGACCATGTGTAGTTATGACTTCCTCATCTATGGAAAAACCCCTTTGTTTAGAGGTAACATATTGGTGCCTGAGTCCAAAAGATGGGCCAGAGTCTCTTGCGTCTCTAATGACCAAATAGTCAACCTCGCCAATATAATGTCGACAGTGTCCACCAATGCGCTAACAGTGGCACAACACTCTCAATCTTTGATCAAACCGATGAGGGATTTTCTGCTCATGTCAGTACAGGCAGTCTTTCACTACCTGCTATTTAGCCCAATCTTAAAGGGAAGAGTTTACAAGATTCTGAGCGCTGAAGGGGAGAGCTTTCTCCTAGCCATGTCAAGGATAATCTATCTAGATCCTTCTTTGGGAGGGATATCTGGAATGTCCCTCGGAAGATTCCATATACGACAGTTCTCAGACCCTGTCTCTGAAGGGTTATCCTTCTGGAGAGAGATCTGGTTAAGCTCCCAAGAGTCCTGGATTCACGCGTTGTGTCAAGAGGCTGGAAACCCAGATCTTGGAGAGAGAACACTCGAGAGCTTCACTCGCCTTCTAGAAGATCCGACCACCTTAAATATCAGAGGAGGGGCCAGTCCTACCATTCTACTCAAGGATGCAATCAGAAAGGCTTTATATGACGAGGTGGACAAGGTGGAAAATTCAGAGTTTCGAGAGGCAATCCTGTTGTCCAAGACCCATAGAGATAATTTTATACTCTTCTTAATATCTGTTGAGCCTCTGTTTCCTCGATTTCTCAGTGAGCTATTCAGTTCGTCTTTTTTGGGAATCCCCGAGTCAATCATTGGATTGATACAAAACTCCCGAACGATAAGAAGGCAGTTTAGAAAGAGTCTCTCAAAAACTTTAGAAGAATCCTTCTACAACTCAGAGATCCACGGGATTAGTCGGATGACCCAGACACCTCAGAGGGTTGGGGGGGTGTGGCCTTGCTCTTCAGAGAGGGCAGATCTACTTAGGGAGATCTCTTGGGGAAGAAAAGTGGTAGGCACGACAGTTCCTCACCCTTCTGAGATGTTGGGATTACTTCCCAAGTCCTCTATTTCTTGCACTTGTGGAGCAACAGGAGGAGGCAATCCTAGAGTTTCTGTATCAGTACTCCCGTCCTTTGATCAGTCATTTTTTTCACGAGGCCCCCTAAAGGGATACTTGGGCTCGTCCACCTCTATGTCGACCCAGCTATTCCATGCATGGGAAAAAGTCACTAATGTTCATGTGGTGAAGAGAGCTCTATCGTTAAAAGAATCTATAAACTGGTTCATTACTAGAGATTCCAACTTGGCTCAAGCTCTAATTAGGAACATTATGTCTCTGACAGGCCCTGATTTCCCTCTAGAGGAGGCCCCTGTCTTCAAAAGGACGGGGTCAGCCTTGCATAGGTTCAAGTCTGCCAGATACAGCGAAGGAGGGTATTCTTCTGTCTGCCCGAACCTCCTCTCTCATATTTCTGTTAGTACAGACACCATGTCTGATTTGACCCAAGACGGGAAGAACTACGATTTCATGTTCCAGCCATTGATGCTTTATGCACAGACATGGACATCAGAGCTGGTACAGAGAGACACAAGGCTAAGAGACTCTACGTTTCATTGGCACCTCCGATGCAACAGGTGTGTGAGACCCATTGACGACGTGACCCTGGAGACCTCTCAGATCTTCGAGTTTCCGGATGTGTCGAAAAGAATATCCAGAATGGTTTCTGGGGCTGTGCCTCACTTCCAGAGGCTTCCCGATATCCGTCTGAGACCAGGAGATTTTGAATCTCTAAGCGGTAGAGAAAAGTCTCACCATATCGGATCAGCTCAGGGGCTCTTATACTCAATCTTAGTGGCAATTCACGACTCAGGATACAATGATGGAACCATCTTCCCTGTCAACATATACGGCAAGGTTTCCCCTAGAGACTATTTGAGAGGGCTCGCAAGGGGAGTATTGATAGGATCCTCGATTTGCTTCTTGACAAGAATGACAAATATCAATATTAATAGACCTCTTGAATTGGTCTCAGGGGTAATCTCATATATTCTCCTGAGGCTAGATAACCATCCCTCCTTGTACATAATGCTCAGAGAACCGTCTCTTAGAGGAGAGATATTTTCTATCCCTCAGAAAATCCCCGCCGCTTATCCAACCACTATGAAAGAAGGCAACAGATCAATCTTGTGTTATCTCCAACATGTGCTACGCTATGAGCGAGAGATAATCACGGCGTCTCCAGAGAATGACTGGCTATGGATCTTTTCAGACTTTAGAAGTGCCAAAATGACGTACCTATCCCTCATTACTTACCAGTCTCATCTTCTACTCCAGAGGGTTGAGAGAAACCTATCTAAGAGTATGAGAGATAACCTGCGACAATTGAGTTCTTTGATGAGGCAGGTGCTGGGCGGGCACGGAGAAGATACCTTAGAGTCAGACGACAACATTCAACGACTGCTAAAAGACTCTTTACGAAGGACAAGATGGGTGGATCAAGAGGTGCGCCATGCAGCTAGAACCATGACTGGAGATTACAGCCCCAACAAGAAGGTGTCCCGTAAGGTAGGATGTTCAGAATGGGTCTGCTCTGCTCAACAGGTTGCAGTCTCTACCTCAGCAAACCCGGCCCCTGTCTCGGAGCTTGACATAAGGGCCCTCTCTAAGAGGTTCCAGAACCCTTTGATCTCGGGCTTGAGAGTGGTTCAGTGGGCAACCGGTGCTCATTATAAGCTTAAGCCTATTCTAGATGATCTCAATGTTTTCCCATCTCTCTGCCTTGTAGTTGGGGACGGGTCAGGGGGGATATCAAGGGCAGTCCTCAACATGTTTCCAGATGCCAAGCTTGTGTTCAACAGTCTTTTAGAGGTGAATGACCTGATGGCTTCCGGAACACATCCACTGCCTCCTTCAGCAATCATGAGGGGAGGAAATGATATCGTCTCCAGAGTGATAGATCTTGACTCAATCTGGGAAAAACCGTCCGACTTGAGAAACTTGGCAACCTGGAAATACTTCCAGTCAGTCCAAAAGCAGGTCAACATGTCCTATGACCTCATTATTTGCGATGCAGAAGTTACTGACATTGCATCTATCAACCGGATCACCCTGTTAATGTCCGATTTTGCATTGTCTATAGATGGACCACTCTATTTGGTCTTCAAAACTTATGGGACTATGCTAGTAAATCCAAACTACAAGGCTATTCAACACCTGTCAAGAGCGTTCCCCTCGGTCACAGGGTTTATCACCCAAGTAACTTCGTCTTTTTCATCTGAGCTCTACCTCCGATTCTCCAAACGAGGGAAGTTTTTCAGAGATGCTGAGTACTTGACCTCTTCCACCCTTCGAGAAATGAGCCTTGTGTTATTCAATTGTAGCAGCCCCAAGAGTGAGATGCAGAGAGCTCGTTCCTTGAACTATCAGGATCTTGTGAGAGGATTTCCTGAAGAAATCATATCAAATCCTTACAATGAGATGATCATAACTCTGATTGACAGTGATGTAGAATCTTTTCTAGTCCACAAGATGGTTGATGATCTTGAGTTACAGAGGGGAACTCTGTCTAAAGTGGCTATCATTATAGCCATCATGATAGTTTTCTCCAACAGAGTCTTCAACGTTTCCAAACCCCTAACTGACCCCTCGTTCTATCCACCGTCTGATCCCAAAATCCTGAGGCACTTCAACATATGTTGCAGTACTATGATGTATCTATCTACTGCTTTAGGTGACGTCCCTAGCTTCGCAAGACTTCACGACCTGTATAACAGACCTATAACTTATTACTTCAGAAAGCAAGTCATTCGAGGGAACGTTTATCTATCTTGGAGTTGGTCCAACGACACCTCAGTGTTCAAAAGGGTAGCCTGTAATTCTAGCCTGAGTCTGTCATCTCACTGGATCAGGTTGATTTACAAGATAGTGAAGACTACCAGACTCGTTGGCAGCATCAAGGATCTATCCAGAGAAGTGGAAAGACACCTTCATAGGTACAACAGGTGGATCACCCTAGAGGATATCAGATCTAGATCATCCCTACTAGACTACAGTTGCCTGTGAACCGGATACTCCTGGAAGCCTGCCCATGCTAAGACTCTTGTGTGATGTATCTTGAAAAAAACAAGATCCTAAATCTGAACCTTTGGTTGTTTGATTGTTTTTCTCATTTTTGTTGTTTATTTGTTAAGCGT。
在一个实施方式中，核酸包括SEQ ID NO：17的序列，如下所示(RABV载体：CoravaxV6 South Africa)：
ACGCTTAACAACCAGATCAAAGAAAAAACAGACATTGTCAATTGCAAAGCAAAAATGTAACACCCCTACAATGGATGCCGACAAGATTGTATTCAAAGTCAATAATCAGGTGGTCTCTTTGAAGCCTGAGATTATCGTGGATCAATATGAGTACAAGTACCCTGCCATCAAAGATTTGAAAAAGCCCTGTATAACCCTAGGAAAGGCTCCCGATTTAAATAAAGCATACAAGTCAGTTTTGTCAGGCATGAGCGCCGCCAAACTTAATCCTGACGATGTATGTTCCTATTTGGCAGCGGCAATGCAGTTTTTTGAGGGGACATGTCCGGAAGACTGGACCAGCTATGGAATTGTGATTGCACGAAAAGGAGATAAGATCACCCCAGGTTCTCTGGTGGAGATAAAACGTACTGATGTAGAAGGGAATTGGGCTCTGACAGGAGGCATGGAACTGACAAGAGACCCCACTGTCCCTGAGCATGCGTCCTTAGTCGGTCTTCTCTTGAGTCTGTATAGGTTGAGCAAAATATCCGGGCAAAACACTGGTAACTATAAGACAAACATTGCAGACAGGATAGAGCAGATTTTTGAGACAGCCCCTTTTGTTAAAATCGTGGAACACCATACTCTAATGACAACTCACAAAATGTGTGCTAATTGGAGTACTATACCAAACTTCAGATTTTTGGCCGGAACCTATGACATGTTTTTCTCCCGGATTGAGCATCTATATTCAGCAATCAGAGTGGGCACAGTTGTCACTGCTTATGAAGACTGTTCAGGACTGGTATCATTTACTGGGTTCATAAAACAAATCAATCTCACCGCTAGAGAGGCAATACTATATTTCTTCCACAAGAACTTTGAGGAAGAGATAAGAAGAATGTTTGAGCCAGGGCAGGAGACAGCTGTTCCTCACTCTTATTTCATCCACTTCCGTTCACTAGGCTTGAGTGGGAAATCTCCTTATTCATCAAATGCTGTTGGTCACGTGTTCAATCTCATTCACTTTGTAGGATGCTATATGGGTCAAGTCAGATCCCTAAATGCAACGGTTATTGCTGCATGTGCTCCTCATGAAATGTCTGTTCTAGGGGGCTATCTGGGAGAGGAATTCTTCGGGAAAGGGACATTTGAAAGAAGATTCTTCAGAGATGAGAAAGAACTTCAAGAATACGAGGCGGCTGAACTGACAAAGACTGACGTAGCACTGGCAGATGATGGAACTGTCAACTCTGACGACGAGGACTACTTTTCAGGTGAAACCAGAAGTCCGGAGGCTGTTTATACTCGAATCATGATGAATGGAGGTCGACTAAAGAGATCTCACATACGGAGATATGTCTCAGTCAGTTCCAATCATCAAGCCCGTCCAAACTCATTCGCCGAGTTTCTAAACAAGACATATTCGAGTGACTCATAAGAAGTTGAATAACAAAATGCCGGAAATCTACGGATTGTGTATATCCATCATGAAAAAAACTAACACCCCTCCTTTCGAACCATCCCAAACATGAGCAAGATCTTTGTCAATCCTAGTGCTATTAGAGCCGGTCTGGCCGATCTTGAGATGGCTGAAGAAACTGTTGATCTGATCAATAGAAATATCGAAGACAATCAGGCTCATCTCCAAGGGGAACCCATAGAGGTGGACAATCTCCCTGAGGATATGGGGCGACTTCACCTGGATGATGGAAAATCGCCCAACCATGGTGAGATAGCCAAGGTGGGAGAAGGCAAGTATCGAGAGGACTTTCAGATGGATGAAGGAGAGGATCCTAGCTTCCTGTTCCAGTCATACCTGGAAAATGTTGGAGTCCAAATAGTCAGACAAATGAGGTCAGGAGAGAGATTTCTCAAGATATGGTCACAGACCGTAGAAGAGATTATATCCTATGTCGCGGTCAACTTTCCCAACCCTCCAGGAAAGTCTTCAGAGGATAAATCAACCCAGACTACTGGCCGAGAGCTCAAGAAGGAGACAACACCCACTCCTTCTCAGAGAGAAAGCCAATCATCGAAAGCCAGGATGGCGGCTCAAATTGCTTCTGGCCCTCCAGCCCTTGAATGGTCGGCTACCAATGAAGAGGATGATCTATCAGTGGAGGCTGAGATCGCTCACCAGATTGCAGAAAGTTTCTCCAAAAAATATAAGTTTCCCTCTCGATCCTCAGGGATACTCTTGTATAATTTTGAGCAATTGAAAATGAACCTTGATGATATAGTTAAAGAGGCAAAAAATGTACCAGGTGTGACCCGTTTAGCCCATGACGGGTCCAAACTCCCCCTAAGATGTGTACTGGGATGGGTCGCTTTGGCCAACTCTAAGAAATTCCAGTTGTTAGTCGAATCCGACAAGCTGAGTAAAATCATGCAAGATGACTTGAATCGCTATACATCTTGCTAACCGAACCTCTCCCCTCAGTCCCTCTAGACAATAAAATCCGAGATGTCCCAAAGTCAACATGAAAAAAACAGGCAACACCACTGATAAAATGAACCTCCTACGTAAGATAGTGAAAAACCGCAGGGACGAGGACACTCAAAAATCCTCTCCCGCGTCAGCCCCTCTGGATGACGATGACTTGTGGCTTCCACCCCCTGAATACGTCCCGCTGAAAGAACTTACAGGCAAGAAGAACATGAGGAACTTTTGTATCAACGGAAGGGTTAAAGTGTGTAGCCCGAATGGTTACTCGTTCAGGATCCTGCGGCACATTCTGAAATCATTCGACGAGATATATTCTGGGAATCATAGGATGATCGGGTTAGTCAAAGTGGTTATTGGACTGGCTTTGTCAGGATCTCCAGTCCCTGAGGGCCTGAACTGGGTATACAAATTGAGGAGAACCTTTATCTTCCAGTGGGCTGATTCCAGGGGCCCTCTTGAAGGGGAGGAGTTGGAATACTCTCAGGAGATCACTTGGGATGATGATACTGAGTTCGTCGGATTGCAAATAAGAGTGATTGCAAAACAGTGTCATATCCAGGGCAGAGTCTGGTGTATCAACATGAACCCGAGAGCATGTCAACTATGGTCTGACATGTCTCTTCAGACACAAAGGTCCGAAGAGGACAAAGATTCCTCTCTGCTTCTAGAATAATCAGATTATATCCCGCAAATTTATCACTTGTTTACCTCTGGAGGAGAGAACATATGGGCTCAACTCCAACCCTTGGGAGCAATATAACAAAAAACATGTTATGGTGCCATTAAACCGCTGCATTTCATCAAAGTCAAGTTGATTACCTTTACATTTTGATCCTCTTGGATGTGAAAAAAACTATTAACATCCCTCAAAAGACCccgggAAAGATGGTTCCTCAGGCTCTCCTGTTTGTACCCCTTCTGGTTTTTCCATTGTGTTTTGGGAAATTCCCTATTTACACGATACCAGACAAGCTTGGTCCCTGGAGTCCGATTGACATACATCACCTCAGCTGCCCAAACAATTTGGTAGTGGAGGACGAAGGATGCACCAACCTGTCAGGGTTCTCCTACATGGAACTTAAAGTTGGATACATCTTAGCCATAAAAGTGAACGGGTTCACTTGCACAGGCGTTGTGACGGAGGCTGAAACCTACACTAACTTCGTTGGTTATGTCACAACCACGTTCAAAAGAAAGCATTTCCGCCCAACACCAGATGCATGTAGAGCCGCGTACAACTGGAAGATGGCCGGTGACCCCAGATATGAAGAGTCTCTACACAATCCGTACCCTGACTACCGCTGGCTTCGAACTGTAAAAACCACCAAGGAGTCTCTCGTTATCATATCTCCAAGTGTGGCAGATTTGGACCCATATGACAGATCCCTTCACTCGAGGGTCTTCCCTAGCGGGAAGTGCTCAGGAGTAGCGGTGTCTTCTACCTACTGCTCCACTAACCACGATTACACCATTTGGATGCCCGAGAATCCGAGACTAGGGATGTCTTGTGACATTTTTACCAATAGTAGAGGGAAGAGAGCATCCAAAGGGAGTGAGACTTGCGGCTTTGTAGATGAAAGAGGCCTATATAAGTCTTTAAAAGGAGCATGCAAACTCAAGTTATGTGGAGTTCTAGGACTTAGACTTATGGATGGAACATGGGTCTCGATGCAAACATCAAATGAAACCAAATGGTGCCCTCCCGATAAGTTGGTGAACCTGCACGACTTTCGCTCAGACGAAATTGAGCACCTTGTTGTAGAGGAGTTGGTCAGGAAGAGAGAGGAGTGTCTGGATGCACTAGAGTCCATCATGACAACCAAGTCAGTGAGTTTCAGACGTCTCAGTCATTTAAGAAAACTTGTCCCTGGGTTTGGAAAAGCATATACCATATTCAACAAGACCTTGATGGAAGCCGATGCTCACTACAAGTCAGTCgagACTTGGAATGAGATCCTCCCTTCAAAAGGGTGTTTAAGAGTTGGGGGGAGGTGTCATCCTCATGTGAACGGGGTGTTTTTCAATGGTATAATATTAGGACCTGACGGCAATGTCTTAATCCCAGAGATGCAATCATCCCTCCTCCAGCAACATATGGAGTTGTTGGAATCCTCGGTTATCCCCCTTGTGCACCCCCTGGCAGACCCGTCTACCGTTTTCAAGGACGGTGACGAGGCTGAGGATTTTGTTGAAGTTCACCTTCCCGATGTGCACAATCAGGTCTCAGGAGTTGACTTGGGTCTCCCGAACTGGGGGAAGTATGTATTACTGAGTGCAGGGGCCCTGACTGCCTTGATGTTGATAATTTTCCTGATGACATGTTGTAGAAGAGTCAATCGATCAGAACCTACGCAACACAATCTCAGAGGGACAGGGAGGGAGGTGTCAGTCACTCCCCAAAGCGGGAAGATCATATCTTCATGGGAATCACACAAGAGTGGGGGTGAGACCAGACTGTAAttaattaaCGTCCTTTCAACGATCCAAGTCcatgaaaaaaactaacacccctcccgtacgaccATGTTCGTGTTTCTGGTGCTGCTGCCTCTGGTGAGCTCCCAGTGCGTGAACTTCACCACAAGGACCCAGCTGCCCCCTGCCTATACCAATTCCTTCACACGGGGCGTGTACTATCCCGACAAGGTGTTCCGGAGCAGCGTGCTGCACTCCACACAGGATCTGTTTCTGCCTTTCTTTTCTAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAATGGCACAAAGCGGTTCGCCAATCCAGTGCTGCCCTTTAACGATGGCGTGTACTTCGCCTCCACCGAGAAGTCTAACATCATCAGAGGCTGGATCTTTGGCACCACACTGGACAGCAAGACACAGTCCCTGCTGATCGTGAACAATGCCACCAACGTGGTCATCAAGGTGTGCGAGTTCCAGTTTTGTAATGATCCATTCCTGGGCGTGTACTATCACAAGAACAATAAGTCTTGGATGGAGAGCGAGTTTCGCGTGTATTCCTCTGCCAACAATTGCACATTTGAGTACGTGTCCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAATTTCAAGAACCTGAGGGAGTTCGTGTTTAAGAATATCGATGGCTACTTCAAAATCTACTCCAAGCACACCCCAATCAACCTGGTGCGCGGCCTGCCACAGGGCTTCTCTGCCCTGGAGCCACTGGTGGATCTGCCCATCGGCATCAACATCACCCGGTTTCAGACACTGCTGGCCCTGCACAGAAGCTACCTGACACCAGGCGACAGCTCCTCTGGATGGACCGCAGGAGCAGCAGCCTACTATGTGGGCTATCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAATGGCACCATCACAGACGCCGTGGATTGCGCCCTGGATCCCCTGTCTGAGACCAAGTGTACACTGAAGAGCTTTACCGTGGAGAAGGGCATCTATCAGACAAGCAATTTCAGGGTGCAGCCTACCGAGTCCATCGTGCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAATATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGAAGGGCTTTAACTGTTATTTCCCTCTGCAGAGCTACGGCTTCCAGCCAACATATGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACCTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGACTGACCGGAACAGGCGTGCTGACCGAGTCCAACAAGAAGTTCCTGCCTTTTCAGCAGTTCGGCAGGGACATCGCAGATACCACAGACGCCGTGCGCGACCCTCAGACCCTGGAGATCCTGGACATCACACCATGCTCCTTCGGCGGCGTGTCTGTGATCACACCAGGCACCAATACAAGCAACCAGGTGGCCGTGCTGTATCAGGGCGTGAATTGTACCGAGGTGCCAGTGGCAATCCACGCAGATCAGCTGACCCCTACATGGCGGGTGTACTCTACCGGCAGCAACGTGTTCCAGACAAGAGCCGGATGCCTGATCGGAGCAGAGCACGTGAACAATAGCTATGAGTGCGACATCCCTATCGGCGCCGGCATCTGTGCCTCCTACCAGACCCAGACAAACTCCCCAGAATCAAGCGTGATTCCTCTGGTCCATCCACTGGCAGATCCCTCCACAGTGTTCAAAGACGGAGATGAGGCCGAAGACTTTGTGGAAGTCCACCTGCCTGATGTGCATAACCAGGTGTCTGGCGTCGACCTGGGACTGCCAAATTGGGGCAAGTACGTGCTGCTGAGTGCTGGAGCACTGACTGCCCTGATGCTGATCATTTTCCTGATGACCTGCTGTCGGCGCGTGAACAGAAGTGAGCCCACTCAGCACAATCTGCGAGGAACCGGGAGAGAAGTGTCAGTCACACCTCAGAGCGGGAAAATCATTAGTAGTTGGGAATCACATAAAAGCGGGGGCGAGACCAGGCTGggatccggctccggcgagggcaggggaagtctactaacatgcggggacgtggaggaaaatcccggccccATGCTCGATCCTGGAGAGGTCTATGATGACCCTATTGACCCAATCGAGTTAGAGGCTGAACCCAGAGGAACCCCCATTGTCCCCAACATCTTGAGGAACTCTGACTACAATCTCAACTCTCCTTTGATAGAAGATCCTGCTAGACTAATGTTAGAATGGTTAAAAACAGGGAATAGACCTTATCGGATGACTCTAACAGACAATTGCTCCAGGTCTTTCAGAGTTTTGAAAGATTATTTCAAGAAGGTAGATTTGGGTTCTCTCAAGGTGGGCGGAATGGCTGCACAGTCAATGATTTCTCTCTGGTTATATGGTGCCCACTCTGAATCCAACAGGAGCCGGAGATGTATAACAGACTTGGCCCATTTCTATTCCAAGTCGTCCCCCATAGAGAAGCTGTTGAATCTCACGCTAGGAAATAGAGGGCTGAGAATCCCCCCAGAGGGAGTGTTAAGTTGCCTTGAGAGGGTTGATTATGATAATGCATTTGGAAGGTATCTTGCCAACACGTATTCCTCTTACTTGTTCTTCCATGTAATCACCTTATACATGAACGCCCTAGACTGGGATGAAGAAAAGACCATCCTAGCATTATGGAAAGATTTAACCTCAGTGGACATCGGGAAGGACTTGGTAAAGTTCAAAGACCAAATATGGGGACTGCTGATCGTGACAAAGGACTTTGTTTACTCCCAAAGTTCCAATTGTCTTTTTGACAGAAACTACACACTTATGCTAAAAGATCTTTTCTTGTCTCGCTTCAACTCCTTAATGGTCTTGCTCTCTCCCCCAGAGCCCCGATACTCAGATGACTTGATATCTCAACTATGCCAGCTGTACATTGCTGGGGATCAAGTCTTGTCTATGTGTGGAAACTCCGGCTATGAAGTCATCAAAATATTGGAGCCATATGTCGTGAATAGTTTAGTCCAGAGAGCAGAAAAGTTTAGGCCTCTCATTCATTCCTTGGGAGACTTTCCTGTATTTATAAAAGACAAGGTAAGTCAACTTGAAGAGACGTTCGGTCCCTGTGCAAGAAGGTTCTTTAGGGCTCTGGATCAATTCGACAACATACATGACTTGGTTTTTGTGTTTGGCTGTTACAGGCATTGGGGGCACCCATATATAGATTATCGAAAGGGTCTGTCAAAACTATATGATCAGGTTCACCTTAAAAAAATGATAGATAAGTCCTACCAGGAGTGCTTAGCAAGCGACCTAGCCAGGAGGATCCTTAGATGGGGTTTTGATAAGTACTCCAAGTGGTATCTGGATTCAAGATTCCTAGCCCGAGACCACCCCTTGACTCCTTATATCAAAACCCAAACATGGCCACCCAAACATATTGTAGACTTGGTGGGGGATACATGGCACAAGCTCCCGATCACGCAGATCTTTGAGATTCCTGAATCAATGGATCCGTCAGAAATATTGGATGACAAATCACATTCTTTCACCAGAACGAGACTAGCTTCTTGGCTGTCAGAAAACCGAGGGGGGCCTGTTCCTAGCGAAAAAGTTATTATCACGGCCCTGTCTAAGCCGCCTGTCAATCCCCGAGAGTTTCTGAGGTCTATAGACCTCGGAGGATTGCCAGATGAAGACTTGATAATTGGCCTCAAGCCAAAGGAACGGGAATTGAAGATTGAAGGTCGATTCTTTGCTCTAATGTCATGGAATCTAAGATTGTATTTTGTCATCACTGAAAAACTCTTGGCCAACTACATCTTGCCACTTTTTGACGCGCTGACTATGACAGACAACCTGAACAAGGTGTTTAAAAAGCTGATCGACAGGGTCACCGGGCAAGGGCTTTTGGACTATTCAAGGGTCACATATGCATTTCACCTGGACTATGAAAAGTGGAACAACCATCAAAGATTAGAGTCAACAGAGGATGTATTTTCTGTCCTAGATCAAGTGTTTGGATTGAAGAGAGTGTTTTCTAGAACACACGAGTTTTTTCAAAAGGCCTGGATCTATTATTCAGACAGATCAGACCTCATCGGGTTACGGGAGGATCAAATATACTGCTTAGATGCGTCCAACGGCCCAACCTGTTGGAATGGCCAGGATGGCGGGCTAGAAGGCTTACGGCAGAAGGGCTGGAGTCTAGTCAGCTTATTGATGATAGATAGAGAATCTCAAATCAGGAACACAAGAACCAAAATACTAGCTCAAGGAGACAACCAGGTTTTATGTCCGACATACATGTTGTCGCCAGGGCTATCTCAAGAGGGGCTCCTCTATGAATTGGAGAGAATATCAAGGAATGCACTTTCGATATACAGAGCCGTCGAGGAAGGGGCATCTAAGCTAGGGCTGATCATCAAGAAAGAAGAGACCATGTGTAGTTATGACTTCCTCATCTATGGAAAAACCCCTTTGTTTAGAGGTAACATATTGGTGCCTGAGTCCAAAAGATGGGCCAGAGTCTCTTGCGTCTCTAATGACCAAATAGTCAACCTCGCCAATATAATGTCGACAGTGTCCACCAATGCGCTAACAGTGGCACAACACTCTCAATCTTTGATCAAACCGATGAGGGATTTTCTGCTCATGTCAGTACAGGCAGTCTTTCACTACCTGCTATTTAGCCCAATCTTAAAGGGAAGAGTTTACAAGATTCTGAGCGCTGAAGGGGAGAGCTTTCTCCTAGCCATGTCAAGGATAATCTATCTAGATCCTTCTTTGGGAGGGATATCTGGAATGTCCCTCGGAAGATTCCATATACGACAGTTCTCAGACCCTGTCTCTGAAGGGTTATCCTTCTGGAGAGAGATCTGGTTAAGCTCCCAAGAGTCCTGGATTCACGCGTTGTGTCAAGAGGCTGGAAACCCAGATCTTGGAGAGAGAACACTCGAGAGCTTCACTCGCCTTCTAGAAGATCCGACCACCTTAAATATCAGAGGAGGGGCCAGTCCTACCATTCTACTCAAGGATGCAATCAGAAAGGCTTTATATGACGAGGTGGACAAGGTGGAAAATTCAGAGTTTCGAGAGGCAATCCTGTTGTCCAAGACCCATAGAGATAATTTTATACTCTTCTTAATATCTGTTGAGCCTCTGTTTCCTCGATTTCTCAGTGAGCTATTCAGTTCGTCTTTTTTGGGAATCCCCGAGTCAATCATTGGATTGATACAAAACTCCCGAACGATAAGAAGGCAGTTTAGAAAGAGTCTCTCAAAAACTTTAGAAGAATCCTTCTACAACTCAGAGATCCACGGGATTAGTCGGATGACCCAGACACCTCAGAGGGTTGGGGGGGTGTGGCCTTGCTCTTCAGAGAGGGCAGATCTACTTAGGGAGATCTCTTGGGGAAGAAAAGTGGTAGGCACGACAGTTCCTCACCCTTCTGAGATGTTGGGATTACTTCCCAAGTCCTCTATTTCTTGCACTTGTGGAGCAACAGGAGGAGGCAATCCTAGAGTTTCTGTATCAGTACTCCCGTCCTTTGATCAGTCATTTTTTTCACGAGGCCCCCTAAAGGGATACTTGGGCTCGTCCACCTCTATGTCGACCCAGCTATTCCATGCATGGGAAAAAGTCACTAATGTTCATGTGGTGAAGAGAGCTCTATCGTTAAAAGAATCTATAAACTGGTTCATTACTAGAGATTCCAACTTGGCTCAAGCTCTAATTAGGAACATTATGTCTCTGACAGGCCCTGATTTCCCTCTAGAGGAGGCCCCTGTCTTCAAAAGGACGGGGTCAGCCTTGCATAGGTTCAAGTCTGCCAGATACAGCGAAGGAGGGTATTCTTCTGTCTGCCCGAACCTCCTCTCTCATATTTCTGTTAGTACAGACACCATGTCTGATTTGACCCAAGACGGGAAGAACTACGATTTCATGTTCCAGCCATTGATGCTTTATGCACAGACATGGACATCAGAGCTGGTACAGAGAGACACAAGGCTAAGAGACTCTACGTTTCATTGGCACCTCCGATGCAACAGGTGTGTGAGACCCATTGACGACGTGACCCTGGAGACCTCTCAGATCTTCGAGTTTCCGGATGTGTCGAAAAGAATATCCAGAATGGTTTCTGGGGCTGTGCCTCACTTCCAGAGGCTTCCCGATATCCGTCTGAGACCAGGAGATTTTGAATCTCTAAGCGGTAGAGAAAAGTCTCACCATATCGGATCAGCTCAGGGGCTCTTATACTCAATCTTAGTGGCAATTCACGACTCAGGATACAATGATGGAACCATCTTCCCTGTCAACATATACGGCAAGGTTTCCCCTAGAGACTATTTGAGAGGGCTCGCAAGGGGAGTATTGATAGGATCCTCGATTTGCTTCTTGACAAGAATGACAAATATCAATATTAATAGACCTCTTGAATTGGTCTCAGGGGTAATCTCATATATTCTCCTGAGGCTAGATAACCATCCCTCCTTGTACATAATGCTCAGAGAACCGTCTCTTAGAGGAGAGATATTTTCTATCCCTCAGAAAATCCCCGCCGCTTATCCAACCACTATGAAAGAAGGCAACAGATCAATCTTGTGTTATCTCCAACATGTGCTACGCTATGAGCGAGAGATAATCACGGCGTCTCCAGAGAATGACTGGCTATGGATCTTTTCAGACTTTAGAAGTGCCAAAATGACGTACCTATCCCTCATTACTTACCAGTCTCATCTTCTACTCCAGAGGGTTGAGAGAAACCTATCTAAGAGTATGAGAGATAACCTGCGACAATTGAGTTCTTTGATGAGGCAGGTGCTGGGCGGGCACGGAGAAGATACCTTAGAGTCAGACGACAACATTCAACGACTGCTAAAAGACTCTTTACGAAGGACAAGATGGGTGGATCAAGAGGTGCGCCATGCAGCTAGAACCATGACTGGAGATTACAGCCCCAACAAGAAGGTGTCCCGTAAGGTAGGATGTTCAGAATGGGTCTGCTCTGCTCAACAGGTTGCAGTCTCTACCTCAGCAAACCCGGCCCCTGTCTCGGAGCTTGACATAAGGGCCCTCTCTAAGAGGTTCCAGAACCCTTTGATCTCGGGCTTGAGAGTGGTTCAGTGGGCAACCGGTGCTCATTATAAGCTTAAGCCTATTCTAGATGATCTCAATGTTTTCCCATCTCTCTGCCTTGTAGTTGGGGACGGGTCAGGGGGGATATCAAGGGCAGTCCTCAACATGTTTCCAGATGCCAAGCTTGTGTTCAACAGTCTTTTAGAGGTGAATGACCTGATGGCTTCCGGAACACATCCACTGCCTCCTTCAGCAATCATGAGGGGAGGAAATGATATCGTCTCCAGAGTGATAGATCTTGACTCAATCTGGGAAAAACCGTCCGACTTGAGAAACTTGGCAACCTGGAAATACTTCCAGTCAGTCCAAAAGCAGGTCAACATGTCCTATGACCTCATTATTTGCGATGCAGAAGTTACTGACATTGCATCTATCAACCGGATCACCCTGTTAATGTCCGATTTTGCATTGTCTATAGATGGACCACTCTATTTGGTCTTCAAAACTTATGGGACTATGCTAGTAAATCCAAACTACAAGGCTATTCAACACCTGTCAAGAGCGTTCCCCTCGGTCACAGGGTTTATCACCCAAGTAACTTCGTCTTTTTCATCTGAGCTCTACCTCCGATTCTCCAAACGAGGGAAGTTTTTCAGAGATGCTGAGTACTTGACCTCTTCCACCCTTCGAGAAATGAGCCTTGTGTTATTCAATTGTAGCAGCCCCAAGAGTGAGATGCAGAGAGCTCGTTCCTTGAACTATCAGGATCTTGTGAGAGGATTTCCTGAAGAAATCATATCAAATCCTTACAATGAGATGATCATAACTCTGATTGACAGTGATGTAGAATCTTTTCTAGTCCACAAGATGGTTGATGATCTTGAGTTACAGAGGGGAACTCTGTCTAAAGTGGCTATCATTATAGCCATCATGATAGTTTTCTCCAACAGAGTCTTCAACGTTTCCAAACCCCTAACTGACCCCTCGTTCTATCCACCGTCTGATCCCAAAATCCTGAGGCACTTCAACATATGTTGCAGTACTATGATGTATCTATCTACTGCTTTAGGTGACGTCCCTAGCTTCGCAAGACTTCACGACCTGTATAACAGACCTATAACTTATTACTTCAGAAAGCAAGTCATTCGAGGGAACGTTTATCTATCTTGGAGTTGGTCCAACGACACCTCAGTGTTCAAAAGGGTAGCCTGTAATTCTAGCCTGAGTCTGTCATCTCACTGGATCAGGTTGATTTACAAGATAGTGAAGACTACCAGACTCGTTGGCAGCATCAAGGATCTATCCAGAGAAGTGGAAAGACACCTTCATAGGTACAACAGGTGGATCACCCTAGAGGATATCAGATCTAGATCATCCCTACTAGACTACAGTTGCCTGTGAACCGGATACTCCTGGAAGCCTGCCCATGCTAAGACTCTTGTGTGATGTATCTTGAAAAAAACAAGATCCTAAATCTGAACCTTTGGTTGTTTGATTGTTTTTCTCATTTTTGTTGTTTATTTGTTAAGCGT。
在一个实施方式中，核酸包括SEQ ID NO：18的序列，如下所示(VSV载体：ConvacV1 China)：
ACGAAGACAAACAAACCATTATTATCATTAAAAGGCTCAGGAGAAACTTTAACAGTAATCAAAATGTCTGTTACAGTCAAGAGAATCATTGACAACACAGTCATAGTTCCAAAACTTCCTGCAAATGAGGATCCAGTGGAATACCCGGCAGATTACTTCAGAAAATCAAAGGAGATTCCTCTTTACATCAATACTACAAAAAGTTTGTCAGATCTAAGAGGATATGTCTACCAAGGCCTCAAATCCGGAAATGTATCAATCATACATGTCAACAGCTACTTGTATGGAGCATTAAAGGACATCCGGGGTAAGTTGGATAAAGATTGGTCAAGTTTCGGAATAAACATCGGGAAAGCAGGGGATACAATCGGAATATTTGACCTTGTATCCTTGAAAGCCCTGGACGGCGTACTTCCAGATGGAGTATCGGATGCTTCCAGAACCAGCGCAGATGACAAATGGTTGCCTTTGTATCTACTTGGCTTATACAGAGTGGGCAGAACACAAATGCCTGAATACAGAAAAAAGCTCATGGATGGGCTGACAAATCAATGCAAAATGATCAATGAACAGTTTGAACCTCTTGTGCCAGAAGGTCGTGACATTTTTGATGTGTGGGGAAATGACAGTAATTACACAAAAATTGTCGCTGCAGTGGACATGTTCTTCCACATGTTCAAAAAACATGAATGTGCCTCGTTCAGATACGGAACTATTGTTTCCAGATTCAAAGATTGTGCTGCATTGGCAACATTTGGACACCTCTGCAAAATAACCGGAATGTCTACAGAAGATGTAACGACCTGGATCTTGAACCGAGAAGTTGCAGATGAAATGGTCCAAATGATGCTTCCAGGCCAAGAAATTGACAAGGCCGATTCATACATGCCTTATTTGATCGACTTTGGATTGTCTTCTAAGTCTCCATATTCTTCCGTCAAAAACCCTGCCTTCCACTTCTGGGGGCAATTGACAGCTCTTCTGCTCAGATCCACCAGAGCAAGGAATGCCCGACAGCCTGATGACATTGAGTATACATCTCTTACTACAGCAGGTTTGTTGTACGCTTATGCAGTAGGATCCTCTGCCGACTTGGCACAACAGTTTTGTGTTGGAGATAACAAATACACTCCAGATGATAGTACCGGAGGATTGACGACTAATGCACCGCCACAAGGCAGAGATGTGGTCGAATGGCTCGGATGGTTTGAAGATCAAAACAGAAAACCGACTCCTGATATGATGCAGTATGCGAAAAGAGCAGTCATGTCACTGCAAGGCCTAAGAGAGAAGACAATTGGCAAGTATGCTAAGTCAGAATTTGACAAATGACCCTATAATTCTCAGATCACCTATTATATATTATGCTACATATGAAAAAAACTAACAGATATCATGGATAATCTCACAAAAGTTCGTGAGTATCTCAAGTCCTATTCTCGTCTGGATCAGGCGGTAGGAGAGATAGATGAGATCGAAGCACAACGAGCTGAAAAGTCCAATTATGAGTTGTTCCAAGAGGATGGAGTGGAAGAGCATACTAAGCCCTCTTATTTTCAGGCAGCAGATGATTCTGACACAGAATCTGAACCAGAAATTGAAGACAATCAAGGTTTGTATGCACCAGATCCAGAAGCTGAGCAAGTTGAAGGCTTTATACAGGGGCCTTTAGATGACTATGCAGATGAGGAAGTGGATGTTGTATTTACTTCGGACTGGAAACAGCCTGAGCTTGAATCTGACGAGCATGGAAAGACCTTACGGTTGACATCGCCAGAGGGTTTAAGTGGAGAGCAGAAATCCCAGTGGCTTTCGACGATTAAAGCAGTCGTGCAAAGTGCCAAATACTGGAATCTGGCAGAGTGCACATTTGAAGCATCGGGAGAAGGGGTCATTATGAAGGAGCGCCAGATAACTCCGGATGTATATAAGGTCACTCCAGTGATGAACACACATCCGTCCCAATCAGAAGCAGTATCAGATGTTTGGTCTCTCTCAAAGACATCCATGACTTTCCAACCCAAGAAAGCAAGTCTTCAGCCTCTCACCATATCCTTGGATGAATTGTTCTCATCTAGAGGAGAGTTCATCTCTGTCGGAGGTGACGGACGAATGTCTCATAAAGAGGCCATCCTGCTCGGCCTGAGATACAAAAAGTTGTACAATCAGGCGAGAGTCAAATATTCTCTGTAGACTATGAAAAAAAGTAACAGATATCACGATCTAAGTGTTATCCCAATCCATTCATCATGAGTTCCTTAAAGAAGATTCTCGGTCTGAAGGGGAAAGGTAAGAAATCTAAGAAATTAGGGATCGCACCACCCCCTTATGAAGAGGACACTAGCATGGAGTATGCTCCGAGCGCTCCAATTGACAAATCCTATTTTGGAGTTGACGAGATGGACACCTATGATCCGAATCAATTAAGATATGAGAAATTCTTCTTTACAGTGAAAATGACGGTTAGATCTAATCGTCCGTTCAGAACATACTCAGATGTGGCAGCCGCTGTATCCCATTGGGATCACATGTACATCGGAATGGCAGGGAAACGTCCCTTCTACAAAATCTTGGCTTTTTTGGGTTCTTCTAATCTAAAGGCCACTCCAGCGGTATTGGCAGATCAAGGTCAACCAGAGTATCACACTCACTGCGAAGGCAGGGCTTATTTGCCACATAGGATGGGGAAGACCCCTCCCATGCTCAATGTACCAGAGCACTTCAGAAGACCATTCAATATAGGTCTTTACAAGGGAACGATTGAGCTCACAATGACCATCTACGATGATGAGTCACTGGAAGCAGCTCCTATGATCTGGGATCATTTCAATTCTTCCAAATTTTCTGATTTCAGAGAGAAGGCCTTAATGTTTGGCCTGATTGTCGAGAAAAAGGCATCTGGAGCGTGGGTCCTGGATTCTATCAGCCACTTCAAATGAGCTAGTCTAACTTCTAGCTTCTGAACAATCCCCGGTTTACTCAGTCTCTCCTAATTCCAGCCTCTCGAACAACTAATATCCTGTCTTTTCTATCCCTATGAAAAAAACTAACAGAGATCGATCTGTTTACGCGTCACTATGAAGTGCCTTTTGTACTTAGCCTTTTTATTCATTGGGGTGAATTGCAAGTTCACCATAGTTTTTCCACACAACCAAAAAGGAAACTGGAAAAATGTTCCTTCTAATTACCATTATTGCCCGTCAAGCTCAGATTTAAATTGGCATAATGACTTAATAGGCACAGCCATACAAGTCAAAATGCCCAAGAGTCACAAGGCTATTCAAGCAGACGGTTGGATGTGTCATGCTTCCAAATGGGTCACTACTTGTGATTTCCGCTGGTATGGACCGAAGTATATAACACAGTCCATCCGATCCTTCACTCCATCTGTAGAACAATGCAAGGAAAGCATTGAACAAACGAAACAAGGAACTTGGCTGAATCCAGGCTTCCCTCCTCAAAGTTGTGGATATGCAACTGTGACGGATGCCGAAGCAGTGATTGTCCAGGTGACTCCTCACCATGTGCTGGTTGATGAATACACAGGAGAATGGGTTGATTCACAGTTCATCAACGGAAAATGCAGCAATTACATATGCCCCACTGTCCATAACTCTACAACCTGGCATTCTGACTATAAGGTCAAAGGGCTATGTGATTCTAACCTCATTTCCATGGACATCACCTTCTTCTCAGAGGACGGAGAGCTATCATCCCTGGGAAAGGAGGGCACAGGGTTCAGAAGTAACTACTTTGCTTATGAAACTGGAGGCAAGGCCTGCAAAATGCAATACTGCAAGCATTGGGGAGTCAGACTCCCATCAGGTGTCTGGTTCGAGATGGCTGATAAGGATCTCTTTGCTGCAGCCAGATTCCCTGAATGCCCAGAAGGGTCAAGTATCTCTGCTCCATCTCAGACCTCAGTGGATGTAAGTCTAATTCAGGACGTTGAGAGGATCTTGGATTATTCCCTCTGCCAAGAAACCTGGAGCAAAATCAGAGCGGGTCTTCCAATCTCTCCAGTGGATCTCAGCTATCTTGCTCCTAAAAACCCAGGAACCGGTCCTGCTTTCACCATAATCAATGGTACCCTAAAATACTTTGAGACCAGATACATCAGAGTCGATATTGCTGCTCCAATCCTCTCAAGAATGGTCGGAATGATCAGTGGAACTACCACAGAAAGGGAACTGTGGGATGACTGGGCACCATATGAAGACGTGGAAATTGGACCCAATGGAGTTCTGAGGACCAGTTCAGGATATAAGTTTCCTTTATACATGATTGGACATGGTATGTTGGACTCCGATCTTCATCTTAGCTCAAAGGCTCAGGTGTTCGAACATCCTCACATTCAAGACGCTGCTTCGCAACTTCCTGATGATGAGAGTTTATTTTTTGGTGATACTGGGCTATCCAAAAATCCAATCGAGCTTGTAGAAGGTTGGTTCAGTAGTTGGAAAAGCTCTATTGCCTCTTTTTTCTTTATCATAGGGTTAATCATTGGACTATTCTTGGTTCTCCGAGTTGGTATCCATCTTTGCATTAAATTAAAGCACACCAAGAAAAGACAGATTTATACAGACATAGAGATGAACCGACTTGGAAAGTAACTCAAATCCTGCTAGGTATGAAAAAAACTAACaGATATCACGCTCGAGCGTACGCCACCATGTTCGTGTTTCTGGTGCTGCTGCCTCTGGTGAGCTCCCAGTGCGTGAACCTGACCACAAGGACCCAGCTGCCCCCTGCCTATACCAATTCCTTCACACGGGGCGTGTACTATCCCGACAAGGTGTTCCGGAGCAGCGTGCTGCACTCCACACAGGATCTGTTTCTGCCTTTCTTTTCTAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAATGGCACAAAGCGGTTCGACAATCCAGTGCTGCCCTTTAACGATGGCGTGTACTTCGCCTCCACCGAGAAGTCTAACATCATCAGAGGCTGGATCTTTGGCACCACACTGGACAGCAAGACACAGTCCCTGCTGATCGTGAACAATGCCACCAACGTGGTCATCAAGGTGTGCGAGTTCCAGTTTTGTAATGATCCATTCCTGGGCGTGTACTATCACAAGAACAATAAGTCTTGGATGGAGAGCGAGTTTCGCGTGTATTCCTCTGCCAACAATTGCACATTTGAGTACGTGTCCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAATTTCAAGAACCTGAGGGAGTTCGTGTTTAAGAATATCGATGGCTACTTCAAAATCTACTCCAAGCACACCCCAATCAACCTGGTGCGCGACCTGCCACAGGGCTTCTCTGCCCTGGAGCCACTGGTGGATCTGCCCATCGGCATCAACATCACCCGGTTTCAGACACTGCTGGCCCTGCACAGAAGCTACCTGACACCAGGCGACAGCTCCTCTGGATGGACCGCAGGAGCAGCAGCCTACTATGTGGGCTATCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAATGGCACCATCACAGACGCCGTGGATTGCGCCCTGGATCCCCTGTCTGAGACCAAGTGTACACTGAAGAGCTTTACCGTGGAGAAGGGCATCTATCAGACAAGCAATTTCAGGGTGCAGCCTACCGAGTCCATCGTGCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAAGATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGGAGGGCTTTAACTGTTATTTCCCTCTGCAGAGCTACGGCTTCCAGCCAACAAACGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACCTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGACTGACCGGAACAGGCGTGCTGACCGAGTCCAACAAGAAGTTCCTGCCTTTTCAGCAGTTCGGCAGGGACATCGCAGATACCACAGACGCCGTGCGCGACCCTCAGACCCTGGAGATCCTGGACATCACACCATGCTCCTTCGGCGGCGTGTCTGTGATCACACCAGGCACCAATACAAGCAACCAGGTGGCCGTGCTGTATCAGGACGTGAATTGTACCGAGGTGCCAGTGGCAATCCACGCAGATCAGCTGACCCCTACATGGCGGGTGTACTCTACCGGCAGCAACGTGTTCCAGACAAGAGCCGGATGCCTGATCGGAGCAGAGCACGTGAACAATAGCTATGAGTGCGACATCCCTATCGGCGCCGGCATCTGTGCCTCCTACCAGACCCAGACAAACTCCCCAAGGTCTGTGGGCGATACAGGCCTGTCCAAGAATCCAATCGAGCTGGTAGAGGGCTGGTTCAGCAGTTGGAAAAGCTCCATCGCCTCCTTTTTCTTTATCATCGGCCTGATCATCGGACTGTTCCTGGTGCTCCGCGTGGGTATCCACCTGTGCATCAAGCTGAAGCACACCAAGAAAAGACAGATTTATACAGACATCGAGATGAACCGCCTGGGAAAGTGAGCTAGCCAGATTCTTCATGTTTGGACCAAATCAACTTGTGATACCATGCTCAAAGAGGCCTCAATTATATTTGAGTTTTTAATTTTTATGAAAAAAACTAACAGCAATCATGGAAGTCCACGATTTTGAGACCGACGAGTTCAATGATTTCAATGAAGATGACTATGCCACAAGAGAATTCCTGAATCCCGATGAGCGCATGACGTACTTGAATCATGCTGATTACAATTTGAATTCTCCTCTAATTAGTGATGATATTGACAATTTGATCAGGAAATTCAATTCTCTTCCGATTCCCTCGATGTGGGATAGTAAGAACTGGGATGGAGTTCTTGAGATGTTAACATCATGTCAAGCCAATCCCATCTCAACATCTCAGATGCATAAATGGATGGGAAGTTGGTTAATGTCTGATAATCATGATGCCAGTCAAGGGTATAGTTTTTTACATGAAGTGGACAAAGAGGCAGAAATAACATTTGACGTGGTGGAGACCTTCATCCGCGGCTGGGGCAACAAACCAATTGAATACATCAAAAAGGAAAGATGGACTGACTCATTCAAAATTCTCGCTTATTTGTGTCAAAAGTTTTTGGACTTACACAAGTTGACATTAATCTTAAATGCTGTCTCTGAGGTGGAATTGCTCAACTTGGCGAGGACTTTCAAAGGCAAAGTCAGAAGAAGTTCTCATGGAACGAACATATGCAGGATTAGGGTTCCCAGCTTGGGTCCTACTTTTATTTCAGAAGGATGGGCTTACTTCAAGAAACTTGATATTCTAATGGACCGAAACTTTCTGTTAATGGTCAAAGATGTGATTATAGGGAGGATGCAAACGGTGCTATCCATGGTATGTAGAATAGACAACCTGTTCTCAGAGCAAGACATCTTCTCCCTTCTAAATATCTACAGAATTGGAGATAAAATTGTGGAGAGGCAGGGAAATTTTTCTTATGACTTGATTAAAATGGTGGAACCGATATGCAACTTGAAGCTGATGAAATTAGCAAGAGAATCAAGGCCTTTAGTCCCACAATTCCCTCATTTTGAAAATCATATCAAGACTTCTGTTGATGAAGGGGCAAAAATTGACCGAGGTATAAGATTCCTCCATGATCAGATAATGAGTGTGAAAACAGTGGATCTCACACTGGTGATTTATGGATCGTTCAGACATTGGGGTCATCCTTTTATAGATTATTACACTGGACTAGAAAAATTACATTCCCAAGTAACCATGAAGAAAGATATTGATGTGTCATATGCAAAAGCACTTGCAAGTGATTTAGCTCGGATTGTTCTATTTCAACAGTTCAATGATCATAAAAAGTGGTTCGTGAATGGAGACTTGCTCCCTCATGATCATCCCTTTAAAAGTCATGTTAAAGAAAATACATGGCCCACAGCTGCTCAAGTTCAAGATTTTGGAGATAAATGGCATGAACTTCCGCTGATTAAATGTTTTGAAATACCCGACTTACTAGACCCATCGATAATATACTCTGACAAAAGTCATTCAATGAATAGGTCAGAGGTGTTGAAACATGTCCGAATGAATCCGAACACTCCTATCCCTAGTAAAAAGGTGTTGCAGACTATGTTGGACACAAAGGCTACCAATTGGAAAGAATTTCTTAAAGAGATTGATGAGAAGGGCTTAGATGATGATGATCTAATTATTGGTCTTAAAGGAAAGGAGAGGGAACTGAAGTTGGCAGGTAGATTTTTCTCCCTAATGTCTTGGAAATTGCGAGAATACTTTGTAATTACCGAATATTTGATAAAGACTCATTTCGTCCCTATGTTTAAAGGCCTGACAATGGCGGACGATCTAACTGCAGTCATTAAAAAGATGTTAGATTCCTCATCCGGCCAAGGATTGAAGTCATATGAGGCAATTTGCATAGCCAATCACATTGATTACGAAAAATGGAATAACCACCAAAGGAAGTTATCAAACGGCCCAGTGTTCCGAGTTATGGGCCAGTTCTTAGGTTATCCATCCTTAATCGAGAGAACTCATGAATTTTTTGAGAAAAGTCTTATATACTACAATGGAAGACCAGACTTGATGCGTGTTCACAACAACACACTGATCAATTCAACCTCCCAACGAGTTTGTTGGCAAGGACAAGAGGGTGGACTGGAAGGTCTACGGCAAAAAGGATGGACTATCCTCAATCTACTGGTTATTCAAAGAGAGGCTAAAATCAGAAACACTGCTGTCAAAGTCTTGGCACAAGGTGATAATCAAGTTATTTGCACACAGTATAAAACGAAGAAATCGAGAAACGTTGTAGAATTACAGGGTGCTCTCAATCAAATGGTTTCTAATAATGAGAAAATTATGACTGCAATCAAAATAGGGACAGGGAAGTTAGGACTTTTGATAAATGACGATGAGACTATGCAATCTGCAGATTACTTGAATTATGGAAAAATACCGATTTTCCGTGGAGTGATTAGAGGGTTAGAGACCAAGAGATGGTCACGAGTGACTTGTGTCACCAATGACCAAATACCCACTTGTGCTAATATAATGAGCTCAGTTTCCACAAATGCTCTCACCGTAGCTCATTTTGCTGAGAACCCAATCAATGCCATGATACAGTACAATTATTTTGGGACATTTGCTAGACTCTTGTTGATGATGCATGATCCTGCTCTTCGTCAATCATTGTATGAAGTTCAAGATAAGATACCGGGCTTGCACAGTTCTACTTTCAAATACGCCATGTTGTATTTGGACCCTTCCATTGGAGGAGTGTCGGGCATGTCTTTGTCCAGGTTTTTGATTAGAGCCTTCCCAGATCCCGTAACAGAAAGTCTCTCATTCTGGAGATTCATCCATGTACATGCTCGAAGTGAGCATCTGAAGGAGATGAGTGCAGTATTTGGAAACCCCGAGATAGCCAAGTTTCGAATAACTCACATAGACAAGCTAGTAGAAGATCCAACCTCTCTGAACATCGCTATGGGAATGAGTCCAGCGAACTTGTTAAAGACTGAGGTTAAAAAATGCTTAATCGAATCAAGACAAACCATCAGGAACCAGGTGATTAAGGATGCAACCATATATTTGTATCATGAAGAGGATCGGCTCAGAAGTTTCTTATGGTCAATAAATCCTCTGTTCCCTAGATTTTTAAGTGAATTCAAATCAGGCACTTTTTTGGGAGTCGCAGACGGGCTCATCAGTCTATTTCAAAATTCTCGTACTATTCGGAACTCCTTTAAGAAAAAGTATCATAGGGAATTGGATGATTTGATTGTGAGGAGTGAGGTATCCTCTTTGACACATTTAGGGAAACTTCATTTGAGAAGGGGATCATGTAAAATGTGGACATGTTCAGCTACTCATGCTGACACATTAAGATACAAATCCTGGGGCCGTACAGTTATTGGGACAACTGTACCCCATCCATTAGAAATGTTGGGTCCACAACATCGAAAAGAGACTCCTTGTGCACCATGTAACACATCAGGGTTCAATTATGTTTCTGTGCATTGTCCAGACGGGATCCATGACGTCTTTAGTTCACGGGGACCATTGCCTGCTTATCTAGGGTCTAAAACATCTGAATCTACATCTATTTTGCAGCCTTGGGAAAGGGAAAGCAAAGTCCCACTGATTAAAAGAGCTACACGTCTTAGAGATGCTATCTCTTGGTTTGTTGAACCCGACTCTAAACTAGCAATGACTATACTTTCTAACATCCACTCTTTAACAGGCGAAGAATGGACCAAAAGGCAGCATGGGTTCAAAAGAACAGGGTCTGCCCTTCATAGGTTTTCGACATCTCGGATGAGCCATGGTGGGTTCGCATCTCAGAGCACTGCAGCATTGACCAGGTTGATGGCAACTACAGACACCATGAGGGATCTGGGAGATCAGAATTTCGACTTTTTATTCCAAGCAACGTTGCTCTATGCTCAAATTACCACCACTGTTGCAAGAGACGGATGGATCACCAGTTGTACAGATCATTATCATATTGCCTGTAAGTCCTGTTTGAGACCCATAGAAGAGATCACCCTGGACTCAAGTATGGACTACACGCCCCCAGATGTATCCCATGTGCTGAAGACATGGAGGAATGGGGAAGGTTCGTGGGGACAAGAGATAAAACAGATCTATCCTTTAGAAGGGAATTGGAAGAATTTAGCACCTGCTGAGCAATCCTATCAAGTCGGCAGATGTATAGGTTTTCTATATGGAGACTTGGCGTATAGAAAATCTACTCATGCCGAGGACAGTTCTCTATTTCCTCTATCTATACAAGGTCGTATTAGAGGTCGAGGTTTCTTAAAAGGGTTGCTAGACGGATTAATGAGAGCAAGTTGCTGCCAAGTAATACACCGGAGAAGTCTGGCTCATTTGAAGAGGCCGGCCAACGCAGTGTACGGAGGTTTGATTTACTTGATTGATAAATTGAGTGTATCACCTCCATTCCTTTCTCTTACTAGATCAGGACCTATTAGAGACGAATTAGAAACGATTCCCCACAAGATCCCAACCTCCTATCCGACAAGCAACCGTGATATGGGGGTGATTGTCAGAAATTACTTCAAATACCAATGCCGTCTAATTGAAAAGGGAAAATACAGATCACATTATTCACAATTATGGTTATTCTCAGATGTCTTATCCATAGACTTCATTGGACCATTCTCTATTTCCACCACCCTCTTGCAAATCCTATACAAGCCATTTTTATCTGGGAAAGATAAGAATGAGTTGAGAGAGCTGGCAAATCTTTCTTCATTGCTAAGATCAGGAGAGGGGTGGGAAGACATACATGTGAAATTCTTCACCAAGGACATATTATTGTGTCCAGAGGAAATCAGACATGCTTGCAAGTTCGGGATTGCTAAGGATAATAATAAAGACATGAGCTATCCCCCTTGGGGAAGGGAATCCAGAGGGACAATTACAACAATCCCTGTTTATTATACGACCACCCCTTACCCAAAGATGCTAGAGATGCCTCCAAGAATCCAAAATCCCCTGCTGTCCGGAATCAGGTTGGGCCAATTACCAACTGGCGCTCATTATAAAATTCGGAGTATATTACATGGAATGGGAATCCATTACAGGGACTTCTTGAGTTGTGGAGACGGCTCCGGAGGGATGACTGCTGCATTACTACGAGAAAATGTGCATAGCAGAGGAATATTCAATAGTCTGTTAGAATTATCAGGGTCAGTCATGCGAGGCGCCTCTCCTGAGCCCCCCAGTGCCCTAGAAACTTTAGGAGGAGATAAATCGAGATGTGTAAATGGTGAAACATGTTGGGAATATCCATCTGACTTATGTGACCCAAGGACTTGGGACTATTTCCTCCGACTCAAAGCAGGCTTGGGGCTTCAAATTGATTTAATTGTAATGGATATGGAAGTTCGGGATTCTTCTACTAGCCTGAAAATTGAGACGAATGTTAGAAATTATGTGCACCGGATTTTGGATGAGCAAGGAGTTTTAATCTACAAGACTTATGGAACATATATTTGTGAGAGCGAAAAGAATGCAGTAACAATCCTTGGTCCCATGTTCAAGACGGTCGACTTAGTTCAAACAGAATTTAGTAGTTCTCAAACGTCTGAAGTATATATGGTATGTAAAGGTTTGAAGAAATTAATCGATGAACCCAATCCCGATTGGTCTTCCATCAATGAATCCTGGAAAAACCTGTACGCATTCCAGTCATCAGAACAGGAATTTGCCAGAGCAAAGAAGGTTAGTACATACTTTACCTTGACAGGTATTCCCTCCCAATTCATTCCTGATCCTTTTGTAAACATTGAGACTATGCTACAAATATTCGGAGTACCCACGGGTGTGTCTCATGCGGCTGCCTTAAAATCATCTGATAGACCTGCAGATTTATTGACCATTAGCCTTTTTTATATGGCGATTATATCGTATTATAACATCAATCATATCAGAGTAGGACCGATACCTCCGAACCCCCCATCAGATGGAATTGCACAAAATGTGGGGATCGCTATAACTGGTATAAGCTTTTGGCTGAGTTTGATGGAGAAAGACATTCCACTATATCAACAGTGTTTAGCAGTTATCCAGCAATCATTCCCGATTAGGTGGGAGGCTGTTTCAGTAAAAGGAGGATACAAGCAGAAGTGGAGTACTAGAGGTGATGGGCTCCCAAAAGATACCCGAACTTCAGACTCCTTGGCCCCAATCGGGAACTGGATCAGATCTCTGGAATTGGTCCGAAACCAAGTTCGTCTAAATCCATTCAATGAGATCTTGTTCAATCAGCTATGTCGTACAGTGGATAATCATTTGAAATGGTCAAATTTGCGAAGAAACACAGGAATGATTGAATGGATCAATAGACGAATTTCAAAAGAAGACCGGTCTATACTGATGTTGAAGAGTGACCTACACGAGGAAAACTCTTGGAGAGATTAAAAAATCATGAGGAGACTCCAAACTTTAAGTATGAAAAAAACTTTGATCCTTAAGACCCTCTTGTGGTTTTTATTTTTTATCTGGTTTTGTGGTCTTCGT。
在另一个实施方式中，核酸包括SEQ ID NO：19的序列，如下所示(VSV载体：ConvacV1 South Africa)：
ACGAAGACAAACAAACCATTATTATCATTAAAAGGCTCAGGAGAAACTTTAACAGTAATCAAAATGTCTGTTACAGTCAAGAGAATCATTGACAACACAGTCATAGTTCCAAAACTTCCTGCAAATGAGGATCCAGTGGAATACCCGGCAGATTACTTCAGAAAATCAAAGGAGATTCCTCTTTACATCAATACTACAAAAAGTTTGTCAGATCTAAGAGGATATGTCTACCAAGGCCTCAAATCCGGAAATGTATCAATCATACATGTCAACAGCTACTTGTATGGAGCATTAAAGGACATCCGGGGTAAGTTGGATAAAGATTGGTCAAGTTTCGGAATAAACATCGGGAAAGCAGGGGATACAATCGGAATATTTGACCTTGTATCCTTGAAAGCCCTGGACGGCGTACTTCCAGATGGAGTATCGGATGCTTCCAGAACCAGCGCAGATGACAAATGGTTGCCTTTGTATCTACTTGGCTTATACAGAGTGGGCAGAACACAAATGCCTGAATACAGAAAAAAGCTCATGGATGGGCTGACAAATCAATGCAAAATGATCAATGAACAGTTTGAACCTCTTGTGCCAGAAGGTCGTGACATTTTTGATGTGTGGGGAAATGACAGTAATTACACAAAAATTGTCGCTGCAGTGGACATGTTCTTCCACATGTTCAAAAAACATGAATGTGCCTCGTTCAGATACGGAACTATTGTTTCCAGATTCAAAGATTGTGCTGCATTGGCAACATTTGGACACCTCTGCAAAATAACCGGAATGTCTACAGAAGATGTAACGACCTGGATCTTGAACCGAGAAGTTGCAGATGAAATGGTCCAAATGATGCTTCCAGGCCAAGAAATTGACAAGGCCGATTCATACATGCCTTATTTGATCGACTTTGGATTGTCTTCTAAGTCTCCATATTCTTCCGTCAAAAACCCTGCCTTCCACTTCTGGGGGCAATTGACAGCTCTTCTGCTCAGATCCACCAGAGCAAGGAATGCCCGACAGCCTGATGACATTGAGTATACATCTCTTACTACAGCAGGTTTGTTGTACGCTTATGCAGTAGGATCCTCTGCCGACTTGGCACAACAGTTTTGTGTTGGAGATAACAAATACACTCCAGATGATAGTACCGGAGGATTGACGACTAATGCACCGCCACAAGGCAGAGATGTGGTCGAATGGCTCGGATGGTTTGAAGATCAAAACAGAAAACCGACTCCTGATATGATGCAGTATGCGAAAAGAGCAGTCATGTCACTGCAAGGCCTAAGAGAGAAGACAATTGGCAAGTATGCTAAGTCAGAATTTGACAAATGACCCTATAATTCTCAGATCACCTATTATATATTATGCTACATATGAAAAAAACTAACAGATATCATGGATAATCTCACAAAAGTTCGTGAGTATCTCAAGTCCTATTCTCGTCTGGATCAGGCGGTAGGAGAGATAGATGAGATCGAAGCACAACGAGCTGAAAAGTCCAATTATGAGTTGTTCCAAGAGGATGGAGTGGAAGAGCATACTAAGCCCTCTTATTTTCAGGCAGCAGATGATTCTGACACAGAATCTGAACCAGAAATTGAAGACAATCAAGGTTTGTATGCACCAGATCCAGAAGCTGAGCAAGTTGAAGGCTTTATACAGGGGCCTTTAGATGACTATGCAGATGAGGAAGTGGATGTTGTATTTACTTCGGACTGGAAACAGCCTGAGCTTGAATCTGACGAGCATGGAAAGACCTTACGGTTGACATCGCCAGAGGGTTTAAGTGGAGAGCAGAAATCCCAGTGGCTTTCGACGATTAAAGCAGTCGTGCAAAGTGCCAAATACTGGAATCTGGCAGAGTGCACATTTGAAGCATCGGGAGAAGGGGTCATTATGAAGGAGCGCCAGATAACTCCGGATGTATATAAGGTCACTCCAGTGATGAACACACATCCGTCCCAATCAGAAGCAGTATCAGATGTTTGGTCTCTCTCAAAGACATCCATGACTTTCCAACCCAAGAAAGCAAGTCTTCAGCCTCTCACCATATCCTTGGATGAATTGTTCTCATCTAGAGGAGAGTTCATCTCTGTCGGAGGTGACGGACGAATGTCTCATAAAGAGGCCATCCTGCTCGGCCTGAGATACAAAAAGTTGTACAATCAGGCGAGAGTCAAATATTCTCTGTAGACTATGAAAAAAAGTAACAGATATCACGATCTAAGTGTTATCCCAATCCATTCATCATGAGTTCCTTAAAGAAGATTCTCGGTCTGAAGGGGAAAGGTAAGAAATCTAAGAAATTAGGGATCGCACCACCCCCTTATGAAGAGGACACTAGCATGGAGTATGCTCCGAGCGCTCCAATTGACAAATCCTATTTTGGAGTTGACGAGATGGACACCTATGATCCGAATCAATTAAGATATGAGAAATTCTTCTTTACAGTGAAAATGACGGTTAGATCTAATCGTCCGTTCAGAACATACTCAGATGTGGCAGCCGCTGTATCCCATTGGGATCACATGTACATCGGAATGGCAGGGAAACGTCCCTTCTACAAAATCTTGGCTTTTTTGGGTTCTTCTAATCTAAAGGCCACTCCAGCGGTATTGGCAGATCAAGGTCAACCAGAGTATCACACTCACTGCGAAGGCAGGGCTTATTTGCCACATAGGATGGGGAAGACCCCTCCCATGCTCAATGTACCAGAGCACTTCAGAAGACCATTCAATATAGGTCTTTACAAGGGAACGATTGAGCTCACAATGACCATCTACGATGATGAGTCACTGGAAGCAGCTCCTATGATCTGGGATCATTTCAATTCTTCCAAATTTTCTGATTTCAGAGAGAAGGCCTTAATGTTTGGCCTGATTGTCGAGAAAAAGGCATCTGGAGCGTGGGTCCTGGATTCTATCAGCCACTTCAAATGAGCTAGTCTAACTTCTAGCTTCTGAACAATCCCCGGTTTACTCAGTCTCTCCTAATTCCAGCCTCTCGAACAACTAATATCCTGTCTTTTCTATCCCTATGAAAAAAACTAACAGAGATCGATCTGTTTACGCGTCACTATGAAGTGCCTTTTGTACTTAGCCTTTTTATTCATTGGGGTGAATTGCAAGTTCACCATAGTTTTTCCACACAACCAAAAAGGAAACTGGAAAAATGTTCCTTCTAATTACCATTATTGCCCGTCAAGCTCAGATTTAAATTGGCATAATGACTTAATAGGCACAGCCATACAAGTCAAAATGCCCAAGAGTCACAAGGCTATTCAAGCAGACGGTTGGATGTGTCATGCTTCCAAATGGGTCACTACTTGTGATTTCCGCTGGTATGGACCGAAGTATATAACACAGTCCATCCGATCCTTCACTCCATCTGTAGAACAATGCAAGGAAAGCATTGAACAAACGAAACAAGGAACTTGGCTGAATCCAGGCTTCCCTCCTCAAAGTTGTGGATATGCAACTGTGACGGATGCCGAAGCAGTGATTGTCCAGGTGACTCCTCACCATGTGCTGGTTGATGAATACACAGGAGAATGGGTTGATTCACAGTTCATCAACGGAAAATGCAGCAATTACATATGCCCCACTGTCCATAACTCTACAACCTGGCATTCTGACTATAAGGTCAAAGGGCTATGTGATTCTAACCTCATTTCCATGGACATCACCTTCTTCTCAGAGGACGGAGAGCTATCATCCCTGGGAAAGGAGGGCACAGGGTTCAGAAGTAACTACTTTGCTTATGAAACTGGAGGCAAGGCCTGCAAAATGCAATACTGCAAGCATTGGGGAGTCAGACTCCCATCAGGTGTCTGGTTCGAGATGGCTGATAAGGATCTCTTTGCTGCAGCCAGATTCCCTGAATGCCCAGAAGGGTCAAGTATCTCTGCTCCATCTCAGACCTCAGTGGATGTAAGTCTAATTCAGGACGTTGAGAGGATCTTGGATTATTCCCTCTGCCAAGAAACCTGGAGCAAAATCAGAGCGGGTCTTCCAATCTCTCCAGTGGATCTCAGCTATCTTGCTCCTAAAAACCCAGGAACCGGTCCTGCTTTCACCATAATCAATGGTACCCTAAAATACTTTGAGACCAGATACATCAGAGTCGATATTGCTGCTCCAATCCTCTCAAGAATGGTCGGAATGATCAGTGGAACTACCACAGAAAGGGAACTGTGGGATGACTGGGCACCATATGAAGACGTGGAAATTGGACCCAATGGAGTTCTGAGGACCAGTTCAGGATATAAGTTTCCTTTATACATGATTGGACATGGTATGTTGGACTCCGATCTTCATCTTAGCTCAAAGGCTCAGGTGTTCGAACATCCTCACATTCAAGACGCTGCTTCGCAACTTCCTGATGATGAGAGTTTATTTTTTGGTGATACTGGGCTATCCAAAAATCCAATCGAGCTTGTAGAAGGTTGGTTCAGTAGTTGGAAAAGCTCTATTGCCTCTTTTTTCTTTATCATAGGGTTAATCATTGGACTATTCTTGGTTCTCCGAGTTGGTATCCATCTTTGCATTAAATTAAAGCACACCAAGAAAAGACAGATTTATACAGACATAGAGATGAACCGACTTGGAAAGTAACTCAAATCCTGCTAGGTATGAAAAAAACTAACaGATATCACGCTCGAGCCACCATGTTCGTGTTTCTGGTGCTGCTGCCTCTGGTGAGCTCCCAGTGCGTGAACTTCACCACAAGGACCCAGCTGCCCCCTGCCTATACCAATTCCTTCACACGGGGCGTGTACTATCCCGACAAGGTGTTCCGGAGCAGCGTGCTGCACTCCACACAGGATCTGTTTCTGCCTTTCTTTTCTAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAATGGCACAAAGCGGTTCGCCAATCCAGTGCTGCCCTTTAACGATGGCGTGTACTTCGCCTCCACCGAGAAGTCTAACATCATCAGAGGCTGGATCTTTGGCACCACACTGGACAGCAAGACACAGTCCCTGCTGATCGTGAACAATGCCACCAACGTGGTCATCAAGGTGTGCGAGTTCCAGTTTTGTAATGATCCATTCCTGGGCGTGTACTATCACAAGAACAATAAGTCTTGGATGGAGAGCGAGTTTCGCGTGTATTCCTCTGCCAACAATTGCACATTTGAGTACGTGTCCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAATTTCAAGAACCTGAGGGAGTTCGTGTTTAAGAATATCGATGGCTACTTCAAAATCTACTCCAAGCACACCCCAATCAACCTGGTGCGCGGCCTGCCACAGGGCTTCTCTGCCCTGGAGCCACTGGTGGATCTGCCCATCGGCATCAACATCACCCGGTTTCAGACACTGCTGGCCCTGCACAGAAGCTACCTGACACCAGGCGACAGCTCCTCTGGATGGACCGCAGGAGCAGCAGCCTACTATGTGGGCTATCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAATGGCACCATCACAGACGCCGTGGATTGCGCCCTGGATCCCCTGTCTGAGACCAAGTGTACACTGAAGAGCTTTACCGTGGAGAAGGGCATCTATCAGACAAGCAATTTCAGGGTGCAGCCTACCGAGTCCATCGTGCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAATATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGAAGGGCTTTAACTGTTATTTCCCTCTGCAGAGCTACGGCTTCCAGCCAACATATGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACCTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGACTGACCGGAACAGGCGTGCTGACCGAGTCCAACAAGAAGTTCCTGCCTTTTCAGCAGTTCGGCAGGGACATCGCAGATACCACAGACGCCGTGCGCGACCCTCAGACCCTGGAGATCCTGGACATCACACCATGCTCCTTCGGCGGCGTGTCTGTGATCACACCAGGCACCAATACAAGCAACCAGGTGGCCGTGCTGTATCAGGGCGTGAATTGTACCGAGGTGCCAGTGGCAATCCACGCAGATCAGCTGACCCCTACATGGCGGGTGTACTCTACCGGCAGCAACGTGTTCCAGACAAGAGCCGGATGCCTGATCGGAGCAGAGCACGTGAACAATAGCTATGAGTGCGACATCCCTATCGGCGCCGGCATCTGTGCCTCCTACCAGACCCAGACAAACTCCCCAAGGTCTGTGGGCGATACAGGCCTGTCCAAGAATCCAATCGAGCTGGTAGAGGGCTGGTTCAGCAGTTGGAAAAGCTCCATCGCCTCCTTTTTCTTTATCATCGGCCTGATCATCGGACTGTTCCTGGTGCTCCGCGTGGGTATCCACCTGTGCATCAAGCTGAAGCACACCAAGAAAAGACAGATTTATACAGACATCGAGATGAACCGCCTGGGAAAGTGAGCTAGCCAGATTCTTCATGTTTGGACCAAATCAACTTGTGATACCATGCTCAAAGAGGCCTCAATTATATTTGAGTTTTTAATTTTTATGAAAAAAACTAACAGCAATCATGGAAGTCCACGATTTTGAGACCGACGAGTTCAATGATTTCAATGAAGATGACTATGCCACAAGAGAATTCCTGAATCCCGATGAGCGCATGACGTACTTGAATCATGCTGATTACAATTTGAATTCTCCTCTAATTAGTGATGATATTGACAATTTGATCAGGAAATTCAATTCTCTTCCGATTCCCTCGATGTGGGATAGTAAGAACTGGGATGGAGTTCTTGAGATGTTAACATCATGTCAAGCCAATCCCATCTCAACATCTCAGATGCATAAATGGATGGGAAGTTGGTTAATGTCTGATAATCATGATGCCAGTCAAGGGTATAGTTTTTTACATGAAGTGGACAAAGAGGCAGAAATAACATTTGACGTGGTGGAGACCTTCATCCGCGGCTGGGGCAACAAACCAATTGAATACATCAAAAAGGAAAGATGGACTGACTCATTCAAAATTCTCGCTTATTTGTGTCAAAAGTTTTTGGACTTACACAAGTTGACATTAATCTTAAATGCTGTCTCTGAGGTGGAATTGCTCAACTTGGCGAGGACTTTCAAAGGCAAAGTCAGAAGAAGTTCTCATGGAACGAACATATGCAGGATTAGGGTTCCCAGCTTGGGTCCTACTTTTATTTCAGAAGGATGGGCTTACTTCAAGAAACTTGATATTCTAATGGACCGAAACTTTCTGTTAATGGTCAAAGATGTGATTATAGGGAGGATGCAAACGGTGCTATCCATGGTATGTAGAATAGACAACCTGTTCTCAGAGCAAGACATCTTCTCCCTTCTAAATATCTACAGAATTGGAGATAAAATTGTGGAGAGGCAGGGAAATTTTTCTTATGACTTGATTAAAATGGTGGAACCGATATGCAACTTGAAGCTGATGAAATTAGCAAGAGAATCAAGGCCTTTAGTCCCACAATTCCCTCATTTTGAAAATCATATCAAGACTTCTGTTGATGAAGGGGCAAAAATTGACCGAGGTATAAGATTCCTCCATGATCAGATAATGAGTGTGAAAACAGTGGATCTCACACTGGTGATTTATGGATCGTTCAGACATTGGGGTCATCCTTTTATAGATTATTACACTGGACTAGAAAAATTACATTCCCAAGTAACCATGAAGAAAGATATTGATGTGTCATATGCAAAAGCACTTGCAAGTGATTTAGCTCGGATTGTTCTATTTCAACAGTTCAATGATCATAAAAAGTGGTTCGTGAATGGAGACTTGCTCCCTCATGATCATCCCTTTAAAAGTCATGTTAAAGAAAATACATGGCCCACAGCTGCTCAAGTTCAAGATTTTGGAGATAAATGGCATGAACTTCCGCTGATTAAATGTTTTGAAATACCCGACTTACTAGACCCATCGATAATATACTCTGACAAAAGTCATTCAATGAATAGGTCAGAGGTGTTGAAACATGTCCGAATGAATCCGAACACTCCTATCCCTAGTAAAAAGGTGTTGCAGACTATGTTGGACACAAAGGCTACCAATTGGAAAGAATTTCTTAAAGAGATTGATGAGAAGGGCTTAGATGATGATGATCTAATTATTGGTCTTAAAGGAAAGGAGAGGGAACTGAAGTTGGCAGGTAGATTTTTCTCCCTAATGTCTTGGAAATTGCGAGAATACTTTGTAATTACCGAATATTTGATAAAGACTCATTTCGTCCCTATGTTTAAAGGCCTGACAATGGCGGACGATCTAACTGCAGTCATTAAAAAGATGTTAGATTCCTCATCCGGCCAAGGATTGAAGTCATATGAGGCAATTTGCATAGCCAATCACATTGATTACGAAAAATGGAATAACCACCAAAGGAAGTTATCAAACGGCCCAGTGTTCCGAGTTATGGGCCAGTTCTTAGGTTATCCATCCTTAATCGAGAGAACTCATGAATTTTTTGAGAAAAGTCTTATATACTACAATGGAAGACCAGACTTGATGCGTGTTCACAACAACACACTGATCAATTCAACCTCCCAACGAGTTTGTTGGCAAGGACAAGAGGGTGGACTGGAAGGTCTACGGCAAAAAGGATGGACTATCCTCAATCTACTGGTTATTCAAAGAGAGGCTAAAATCAGAAACACTGCTGTCAAAGTCTTGGCACAAGGTGATAATCAAGTTATTTGCACACAGTATAAAACGAAGAAATCGAGAAACGTTGTAGAATTACAGGGTGCTCTCAATCAAATGGTTTCTAATAATGAGAAAATTATGACTGCAATCAAAATAGGGACAGGGAAGTTAGGACTTTTGATAAATGACGATGAGACTATGCAATCTGCAGATTACTTGAATTATGGAAAAATACCGATTTTCCGTGGAGTGATTAGAGGGTTAGAGACCAAGAGATGGTCACGAGTGACTTGTGTCACCAATGACCAAATACCCACTTGTGCTAATATAATGAGCTCAGTTTCCACAAATGCTCTCACCGTAGCTCATTTTGCTGAGAACCCAATCAATGCCATGATACAGTACAATTATTTTGGGACATTTGCTAGACTCTTGTTGATGATGCATGATCCTGCTCTTCGTCAATCATTGTATGAAGTTCAAGATAAGATACCGGGCTTGCACAGTTCTACTTTCAAATACGCCATGTTGTATTTGGACCCTTCCATTGGAGGAGTGTCGGGCATGTCTTTGTCCAGGTTTTTGATTAGAGCCTTCCCAGATCCCGTAACAGAAAGTCTCTCATTCTGGAGATTCATCCATGTACATGCTCGAAGTGAGCATCTGAAGGAGATGAGTGCAGTATTTGGAAACCCCGAGATAGCCAAGTTTCGAATAACTCACATAGACAAGCTAGTAGAAGATCCAACCTCTCTGAACATCGCTATGGGAATGAGTCCAGCGAACTTGTTAAAGACTGAGGTTAAAAAATGCTTAATCGAATCAAGACAAACCATCAGGAACCAGGTGATTAAGGATGCAACCATATATTTGTATCATGAAGAGGATCGGCTCAGAAGTTTCTTATGGTCAATAAATCCTCTGTTCCCTAGATTTTTAAGTGAATTCAAATCAGGCACTTTTTTGGGAGTCGCAGACGGGCTCATCAGTCTATTTCAAAATTCTCGTACTATTCGGAACTCCTTTAAGAAAAAGTATCATAGGGAATTGGATGATTTGATTGTGAGGAGTGAGGTATCCTCTTTGACACATTTAGGGAAACTTCATTTGAGAAGGGGATCATGTAAAATGTGGACATGTTCAGCTACTCATGCTGACACATTAAGATACAAATCCTGGGGCCGTACAGTTATTGGGACAACTGTACCCCATCCATTAGAAATGTTGGGTCCACAACATCGAAAAGAGACTCCTTGTGCACCATGTAACACATCAGGGTTCAATTATGTTTCTGTGCATTGTCCAGACGGGATCCATGACGTCTTTAGTTCACGGGGACCATTGCCTGCTTATCTAGGGTCTAAAACATCTGAATCTACATCTATTTTGCAGCCTTGGGAAAGGGAAAGCAAAGTCCCACTGATTAAAAGAGCTACACGTCTTAGAGATGCTATCTCTTGGTTTGTTGAACCCGACTCTAAACTAGCAATGACTATACTTTCTAACATCCACTCTTTAACAGGCGAAGAATGGACCAAAAGGCAGCATGGGTTCAAAAGAACAGGGTCTGCCCTTCATAGGTTTTCGACATCTCGGATGAGCCATGGTGGGTTCGCATCTCAGAGCACTGCAGCATTGACCAGGTTGATGGCAACTACAGACACCATGAGGGATCTGGGAGATCAGAATTTCGACTTTTTATTCCAAGCAACGTTGCTCTATGCTCAAATTACCACCACTGTTGCAAGAGACGGATGGATCACCAGTTGTACAGATCATTATCATATTGCCTGTAAGTCCTGTTTGAGACCCATAGAAGAGATCACCCTGGACTCAAGTATGGACTACACGCCCCCAGATGTATCCCATGTGCTGAAGACATGGAGGAATGGGGAAGGTTCGTGGGGACAAGAGATAAAACAGATCTATCCTTTAGAAGGGAATTGGAAGAATTTAGCACCTGCTGAGCAATCCTATCAAGTCGGCAGATGTATAGGTTTTCTATATGGAGACTTGGCGTATAGAAAATCTACTCATGCCGAGGACAGTTCTCTATTTCCTCTATCTATACAAGGTCGTATTAGAGGTCGAGGTTTCTTAAAAGGGTTGCTAGACGGATTAATGAGAGCAAGTTGCTGCCAAGTAATACACCGGAGAAGTCTGGCTCATTTGAAGAGGCCGGCCAACGCAGTGTACGGAGGTTTGATTTACTTGATTGATAAATTGAGTGTATCACCTCCATTCCTTTCTCTTACTAGATCAGGACCTATTAGAGACGAATTAGAAACGATTCCCCACAAGATCCCAACCTCCTATCCGACAAGCAACCGTGATATGGGGGTGATTGTCAGAAATTACTTCAAATACCAATGCCGTCTAATTGAAAAGGGAAAATACAGATCACATTATTCACAATTATGGTTATTCTCAGATGTCTTATCCATAGACTTCATTGGACCATTCTCTATTTCCACCACCCTCTTGCAAATCCTATACAAGCCATTTTTATCTGGGAAAGATAAGAATGAGTTGAGAGAGCTGGCAAATCTTTCTTCATTGCTAAGATCAGGAGAGGGGTGGGAAGACATACATGTGAAATTCTTCACCAAGGACATATTATTGTGTCCAGAGGAAATCAGACATGCTTGCAAGTTCGGGATTGCTAAGGATAATAATAAAGACATGAGCTATCCCCCTTGGGGAAGGGAATCCAGAGGGACAATTACAACAATCCCTGTTTATTATACGACCACCCCTTACCCAAAGATGCTAGAGATGCCTCCAAGAATCCAAAATCCCCTGCTGTCCGGAATCAGGTTGGGCCAATTACCAACTGGCGCTCATTATAAAATTCGGAGTATATTACATGGAATGGGAATCCATTACAGGGACTTCTTGAGTTGTGGAGACGGCTCCGGAGGGATGACTGCTGCATTACTACGAGAAAATGTGCATAGCAGAGGAATATTCAATAGTCTGTTAGAATTATCAGGGTCAGTCATGCGAGGCGCCTCTCCTGAGCCCCCCAGTGCCCTAGAAACTTTAGGAGGAGATAAATCGAGATGTGTAAATGGTGAAACATGTTGGGAATATCCATCTGACTTATGTGACCCAAGGACTTGGGACTATTTCCTCCGACTCAAAGCAGGCTTGGGGCTTCAAATTGATTTAATTGTAATGGATATGGAAGTTCGGGATTCTTCTACTAGCCTGAAAATTGAGACGAATGTTAGAAATTATGTGCACCGGATTTTGGATGAGCAAGGAGTTTTAATCTACAAGACTTATGGAACATATATTTGTGAGAGCGAAAAGAATGCAGTAACAATCCTTGGTCCCATGTTCAAGACGGTCGACTTAGTTCAAACAGAATTTAGTAGTTCTCAAACGTCTGAAGTATATATGGTATGTAAAGGTTTGAAGAAATTAATCGATGAACCCAATCCCGATTGGTCTTCCATCAATGAATCCTGGAAAAACCTGTACGCATTCCAGTCATCAGAACAGGAATTTGCCAGAGCAAAGAAGGTTAGTACATACTTTACCTTGACAGGTATTCCCTCCCAATTCATTCCTGATCCTTTTGTAAACATTGAGACTATGCTACAAATATTCGGAGTACCCACGGGTGTGTCTCATGCGGCTGCCTTAAAATCATCTGATAGACCTGCAGATTTATTGACCATTAGCCTTTTTTATATGGCGATTATATCGTATTATAACATCAATCATATCAGAGTAGGACCGATACCTCCGAACCCCCCATCAGATGGAATTGCACAAAATGTGGGGATCGCTATAACTGGTATAAGCTTTTGGCTGAGTTTGATGGAGAAAGACATTCCACTATATCAACAGTGTTTAGCAGTTATCCAGCAATCATTCCCGATTAGGTGGGAGGCTGTTTCAGTAAAAGGAGGATACAAGCAGAAGTGGAGTACTAGAGGTGATGGGCTCCCAAAAGATACCCGAACTTCAGACTCCTTGGCCCCAATCGGGAACTGGATCAGATCTCTGGAATTGGTCCGAAACCAAGTTCGTCTAAATCCATTCAATGAGATCTTGTTCAATCAGCTATGTCGTACAGTGGATAATCATTTGAAATGGTCAAATTTGCGAAGAAACACAGGAATGATTGAATGGATCAATAGACGAATTTCAAAAGAAGACCGGTCTATACTGATGTTGAAGAGTGACCTACACGAGGAAAACTCTTGGAGAGATTAAAAAATCATGAGGAGACTCCAAACTTTAAGTATGAAAAAAACTTTGATCCTTAAGACCCTCTTGTGGTTTTTATTTTTTATCTGGTTTTGTGGTCTTCGT。
在一些实施方式中，核酸包括SEQ ID NO：20的序列，如下所示(VSV载体：ConvacV2 China)：
ACGAAGACAAACAAACCATTATTATCATTAAAAGGCTCAGGAGAAACTTTAACAGTAATCAAAATGTCTGTTACAGTCAAGAGAATCATTGACAACACAGTCATAGTTCCAAAACTTCCTGCAAATGAGGATCCAGTGGAATACCCGGCAGATTACTTCAGAAAATCAAAGGAGATTCCTCTTTACATCAATACTACAAAAAGTTTGTCAGATCTAAGAGGATATGTCTACCAAGGCCTCAAATCCGGAAATGTATCAATCATACATGTCAACAGCTACTTGTATGGAGCATTAAAGGACATCCGGGGTAAGTTGGATAAAGATTGGTCAAGTTTCGGAATAAACATCGGGAAAGCAGGGGATACAATCGGAATATTTGACCTTGTATCCTTGAAAGCCCTGGACGGCGTACTTCCAGATGGAGTATCGGATGCTTCCAGAACCAGCGCAGATGACAAATGGTTGCCTTTGTATCTACTTGGCTTATACAGAGTGGGCAGAACACAAATGCCTGAATACAGAAAAAAGCTCATGGATGGGCTGACAAATCAATGCAAAATGATCAATGAACAGTTTGAACCTCTTGTGCCAGAAGGTCGTGACATTTTTGATGTGTGGGGAAATGACAGTAATTACACAAAAATTGTCGCTGCAGTGGACATGTTCTTCCACATGTTCAAAAAACATGAATGTGCCTCGTTCAGATACGGAACTATTGTTTCCAGATTCAAAGATTGTGCTGCATTGGCAACATTTGGACACCTCTGCAAAATAACCGGAATGTCTACAGAAGATGTAACGACCTGGATCTTGAACCGAGAAGTTGCAGATGAAATGGTCCAAATGATGCTTCCAGGCCAAGAAATTGACAAGGCCGATTCATACATGCCTTATTTGATCGACTTTGGATTGTCTTCTAAGTCTCCATATTCTTCCGTCAAAAACCCTGCCTTCCACTTCTGGGGGCAATTGACAGCTCTTCTGCTCAGATCCACCAGAGCAAGGAATGCCCGACAGCCTGATGACATTGAGTATACATCTCTTACTACAGCAGGTTTGTTGTACGCTTATGCAGTAGGATCCTCTGCCGACTTGGCACAACAGTTTTGTGTTGGAGATAACAAATACACTCCAGATGATAGTACCGGAGGATTGACGACTAATGCACCGCCACAAGGCAGAGATGTGGTCGAATGGCTCGGATGGTTTGAAGATCAAAACAGAAAACCGACTCCTGATATGATGCAGTATGCGAAAAGAGCAGTCATGTCACTGCAAGGCCTAAGAGAGAAGACAATTGGCAAGTATGCTAAGTCAGAATTTGACAAATGACCCTATAATTCTCAGATCACCTATTATATATTATGCTACATATGAAAAAAACTAACAGATATCATGGATAATCTCACAAAAGTTCGTGAGTATCTCAAGTCCTATTCTCGTCTGGATCAGGCGGTAGGAGAGATAGATGAGATCGAAGCACAACGAGCTGAAAAGTCCAATTATGAGTTGTTCCAAGAGGATGGAGTGGAAGAGCATACTAAGCCCTCTTATTTTCAGGCAGCAGATGATTCTGACACAGAATCTGAACCAGAAATTGAAGACAATCAAGGTTTGTATGCACCAGATCCAGAAGCTGAGCAAGTTGAAGGCTTTATACAGGGGCCTTTAGATGACTATGCAGATGAGGAAGTGGATGTTGTATTTACTTCGGACTGGAAACAGCCTGAGCTTGAATCTGACGAGCATGGAAAGACCTTACGGTTGACATCGCCAGAGGGTTTAAGTGGAGAGCAGAAATCCCAGTGGCTTTCGACGATTAAAGCAGTCGTGCAAAGTGCCAAATACTGGAATCTGGCAGAGTGCACATTTGAAGCATCGGGAGAAGGGGTCATTATGAAGGAGCGCCAGATAACTCCGGATGTATATAAGGTCACTCCAGTGATGAACACACATCCGTCCCAATCAGAAGCAGTATCAGATGTTTGGTCTCTCTCAAAGACATCCATGACTTTCCAACCCAAGAAAGCAAGTCTTCAGCCTCTCACCATATCCTTGGATGAATTGTTCTCATCTAGAGGAGAGTTCATCTCTGTCGGAGGTGACGGACGAATGTCTCATAAAGAGGCCATCCTGCTCGGCCTGAGATACAAAAAGTTGTACAATCAGGCGAGAGTCAAATATTCTCTGTAGACTATGAAAAAAAGTAACAGATATCACGATCTAAGTGTTATCCCAATCCATTCATCATGAGTTCCTTAAAGAAGATTCTCGGTCTGAAGGGGAAAGGTAAGAAATCTAAGAAATTAGGGATCGCACCACCCCCTTATGAAGAGGACACTAGCATGGAGTATGCTCCGAGCGCTCCAATTGACAAATCCTATTTTGGAGTTGACGAGATGGACACCTATGATCCGAATCAATTAAGATATGAGAAATTCTTCTTTACAGTGAAAATGACGGTTAGATCTAATCGTCCGTTCAGAACATACTCAGATGTGGCAGCCGCTGTATCCCATTGGGATCACATGTACATCGGAATGGCAGGGAAACGTCCCTTCTACAAAATCTTGGCTTTTTTGGGTTCTTCTAATCTAAAGGCCACTCCAGCGGTATTGGCAGATCAAGGTCAACCAGAGTATCACACTCACTGCGAAGGCAGGGCTTATTTGCCACATAGGATGGGGAAGACCCCTCCCATGCTCAATGTACCAGAGCACTTCAGAAGACCATTCAATATAGGTCTTTACAAGGGAACGATTGAGCTCACAATGACCATCTACGATGATGAGTCACTGGAAGCAGCTCCTATGATCTGGGATCATTTCAATTCTTCCAAATTTTCTGATTTCAGAGAGAAGGCCTTAATGTTTGGCCTGATTGTCGAGAAAAAGGCATCTGGAGCGTGGGTCCTGGATTCTATCAGCCACTTCAAATGAGCTAGTCTAACTTCTAGCTTCTGAACAATCCCCGGTTTACTCAGTCTCTCCTAATTCCAGCCTCTCGAACAACTAATATCCTGTCTTTTCTATCCCTATGAAAAAAACTAACAGAGATCGATCTGTTTACGCGTCACTATGAAGTGCCTTTTGTACTTAGCCTTTTTATTCATTGGGGTGAATTGCAAGTTCACCATAGTTTTTCCACACAACCAAAAAGGAAACTGGAAAAATGTTCCTTCTAATTACCATTATTGCCCGTCAAGCTCAGATTTAAATTGGCATAATGACTTAATAGGCACAGCCATACAAGTCAAAATGCCCAAGAGTCACAAGGCTATTCAAGCAGACGGTTGGATGTGTCATGCTTCCAAATGGGTCACTACTTGTGATTTCCGCTGGTATGGACCGAAGTATATAACACAGTCCATCCGATCCTTCACTCCATCTGTAGAACAATGCAAGGAAAGCATTGAACAAACGAAACAAGGAACTTGGCTGAATCCAGGCTTCCCTCCTCAAAGTTGTGGATATGCAACTGTGACGGATGCCGAAGCAGTGATTGTCCAGGTGACTCCTCACCATGTGCTGGTTGATGAATACACAGGAGAATGGGTTGATTCACAGTTCATCAACGGAAAATGCAGCAATTACATATGCCCCACTGTCCATAACTCTACAACCTGGCATTCTGACTATAAGGTCAAAGGGCTATGTGATTCTAACCTCATTTCCATGGACATCACCTTCTTCTCAGAGGACGGAGAGCTATCATCCCTGGGAAAGGAGGGCACAGGGTTCAGAAGTAACTACTTTGCTTATGAAACTGGAGGCAAGGCCTGCAAAATGCAATACTGCAAGCATTGGGGAGTCAGACTCCCATCAGGTGTCTGGTTCGAGATGGCTGATAAGGATCTCTTTGCTGCAGCCAGATTCCCTGAATGCCCAGAAGGGTCAAGTATCTCTGCTCCATCTCAGACCTCAGTGGATGTAAGTCTAATTCAGGACGTTGAGAGGATCTTGGATTATTCCCTCTGCCAAGAAACCTGGAGCAAAATCAGAGCGGGTCTTCCAATCTCTCCAGTGGATCTCAGCTATCTTGCTCCTAAAAACCCAGGAACCGGTCCTGCTTTCACCATAATCAATGGTACCCTAAAATACTTTGAGACCAGATACATCAGAGTCGATATTGCTGCTCCAATCCTCTCAAGAATGGTCGGAATGATCAGTGGAACTACCACAGAAAGGGAACTGTGGGATGACTGGGCACCATATGAAGACGTGGAAATTGGACCCAATGGAGTTCTGAGGACCAGTTCAGGATATAAGTTTCCTTTATACATGATTGGACATGGTATGTTGGACTCCGATCTTCATCTTAGCTCAAAGGCTCAGGTGTTCGAACATCCTCACATTCAAGACGCTGCTTCGCAACTTCCTGATGATGAGAGTTTATTTTTTGGTGATACTGGGCTATCCAAAAATCCAATCGAGCTTGTAGAAGGTTGGTTCAGTAGTTGGAAAAGCTCTATTGCCTCTTTTTTCTTTATCATAGGGTTAATCATTGGACTATTCTTGGTTCTCCGAGTTGGTATCCATCTTTGCATTAAATTAAAGCACACCAAGAAAAGACAGATTTATACAGACATAGAGATGAACCGACTTGGAAAGTAACTCAAATCCTGCTAGGTATGAAAAAAACTAACaGATATCACGCTCGAGCGTACGCCACCATGTTCGTGTTTCTGGTGCTGCTGCCTCTGGTGAGCTCCCAGTGCGTGAACCTGACCACAAGGACCCAGCTGCCCCCTGCCTATACCAATTCCTTCACACGGGGCGTGTACTATCCCGACAAGGTGTTCCGGAGCAGCGTGCTGCACTCCACACAGGATCTGTTTCTGCCTTTCTTTTCTAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAATGGCACAAAGCGGTTCGACAATCCAGTGCTGCCCTTTAACGATGGCGTGTACTTCGCCTCCACCGAGAAGTCTAACATCATCAGAGGCTGGATCTTTGGCACCACACTGGACAGCAAGACACAGTCCCTGCTGATCGTGAACAATGCCACCAACGTGGTCATCAAGGTGTGCGAGTTCCAGTTTTGTAATGATCCATTCCTGGGCGTGTACTATCACAAGAACAATAAGTCTTGGATGGAGAGCGAGTTTCGCGTGTATTCCTCTGCCAACAATTGCACATTTGAGTACGTGTCCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAATTTCAAGAACCTGAGGGAGTTCGTGTTTAAGAATATCGATGGCTACTTCAAAATCTACTCCAAGCACACCCCAATCAACCTGGTGCGCGACCTGCCACAGGGCTTCTCTGCCCTGGAGCCACTGGTGGATCTGCCCATCGGCATCAACATCACCCGGTTTCAGACACTGCTGGCCCTGCACAGAAGCTACCTGACACCAGGCGACAGCTCCTCTGGATGGACCGCAGGAGCAGCAGCCTACTATGTGGGCTATCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAATGGCACCATCACAGACGCCGTGGATTGCGCCCTGGATCCCCTGTCTGAGACCAAGTGTACACTGAAGAGCTTTACCGTGGAGAAGGGCATCTATCAGACAAGCAATTTCAGGGTGCAGCCTACCGAGTCCATCGTGCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAAGATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGGAGGGCTTTAACTGTTATTTCCCTCTGCAGAGCTACGGCTTCCAGCCAACAAACGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACCTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGACTGACCGGAACAGGCGTGCTGACCGAGTCCAACAAGAAGTTCCTGCCTTTTCAGCAGTTCGGCAGGGACATCGCAGATACCACAGACGCCGTGCGCGACCCTCAGACCCTGGAGATCCTGGACATCACACCATGCTCCTTCGGCGGCGTGTCTGTGATCACACCAGGCACCAATACAAGCAACCAGGTGGCCGTGCTGTATCAGGACGTGAATTGTACCGAGGTGCCAGTGGCAATCCACGCAGATCAGCTGACCCCTACATGGCGGGTGTACTCTACCGGCAGCAACGTGTTCCAGACAAGAGCCGGATGCCTGATCGGAGCAGAGCACGTGAACAATAGCTATGAGTGCGACATCCCTATCGGCGCCGGCATCTGTGCCTCCTACCAGACCCAGACAAACTCCCCAAGGTCTGTGGGAGATGAGGCCGAAGACTTTGTGGAAGTCCACCTGCCTGATGTGCATAACCAGGTGTCTGGCGTCGACCTGGGACTGCCAAATTGGGGCAAGTACGTGCTGCTGAGTGCTGGAGCACTGACTGCCCTGATGCTGATCATTTTCCTGATGACCTGCTGTCGGCGCGTGAACAGAAGTGAGCCCACTCAGCACAATCTGCGAGGAACCGGGAGAGAAGTGTCAGTCACACCTCAGAGCGGGAAAATCATTAGTAGTTGGGAATCACATAAAAGCGGGGGCGAGACCAGGCTGTGAGCTAGCCAGATTCTTCATGTTTGGACCAAATCAACTTGTGATACCATGCTCAAAGAGGCCTCAATTATATTTGAGTTTTTAATTTTTATGAAAAAAACTAACAGCAATCATGGAAGTCCACGATTTTGAGACCGACGAGTTCAATGATTTCAATGAAGATGACTATGCCACAAGAGAATTCCTGAATCCCGATGAGCGCATGACGTACTTGAATCATGCTGATTACAATTTGAATTCTCCTCTAATTAGTGATGATATTGACAATTTGATCAGGAAATTCAATTCTCTTCCGATTCCCTCGATGTGGGATAGTAAGAACTGGGATGGAGTTCTTGAGATGTTAACATCATGTCAAGCCAATCCCATCTCAACATCTCAGATGCATAAATGGATGGGAAGTTGGTTAATGTCTGATAATCATGATGCCAGTCAAGGGTATAGTTTTTTACATGAAGTGGACAAAGAGGCAGAAATAACATTTGACGTGGTGGAGACCTTCATCCGCGGCTGGGGCAACAAACCAATTGAATACATCAAAAAGGAAAGATGGACTGACTCATTCAAAATTCTCGCTTATTTGTGTCAAAAGTTTTTGGACTTACACAAGTTGACATTAATCTTAAATGCTGTCTCTGAGGTGGAATTGCTCAACTTGGCGAGGACTTTCAAAGGCAAAGTCAGAAGAAGTTCTCATGGAACGAACATATGCAGGATTAGGGTTCCCAGCTTGGGTCCTACTTTTATTTCAGAAGGATGGGCTTACTTCAAGAAACTTGATATTCTAATGGACCGAAACTTTCTGTTAATGGTCAAAGATGTGATTATAGGGAGGATGCAAACGGTGCTATCCATGGTATGTAGAATAGACAACCTGTTCTCAGAGCAAGACATCTTCTCCCTTCTAAATATCTACAGAATTGGAGATAAAATTGTGGAGAGGCAGGGAAATTTTTCTTATGACTTGATTAAAATGGTGGAACCGATATGCAACTTGAAGCTGATGAAATTAGCAAGAGAATCAAGGCCTTTAGTCCCACAATTCCCTCATTTTGAAAATCATATCAAGACTTCTGTTGATGAAGGGGCAAAAATTGACCGAGGTATAAGATTCCTCCATGATCAGATAATGAGTGTGAAAACAGTGGATCTCACACTGGTGATTTATGGATCGTTCAGACATTGGGGTCATCCTTTTATAGATTATTACACTGGACTAGAAAAATTACATTCCCAAGTAACCATGAAGAAAGATATTGATGTGTCATATGCAAAAGCACTTGCAAGTGATTTAGCTCGGATTGTTCTATTTCAACAGTTCAATGATCATAAAAAGTGGTTCGTGAATGGAGACTTGCTCCCTCATGATCATCCCTTTAAAAGTCATGTTAAAGAAAATACATGGCCCACAGCTGCTCAAGTTCAAGATTTTGGAGATAAATGGCATGAACTTCCGCTGATTAAATGTTTTGAAATACCCGACTTACTAGACCCATCGATAATATACTCTGACAAAAGTCATTCAATGAATAGGTCAGAGGTGTTGAAACATGTCCGAATGAATCCGAACACTCCTATCCCTAGTAAAAAGGTGTTGCAGACTATGTTGGACACAAAGGCTACCAATTGGAAAGAATTTCTTAAAGAGATTGATGAGAAGGGCTTAGATGATGATGATCTAATTATTGGTCTTAAAGGAAAGGAGAGGGAACTGAAGTTGGCAGGTAGATTTTTCTCCCTAATGTCTTGGAAATTGCGAGAATACTTTGTAATTACCGAATATTTGATAAAGACTCATTTCGTCCCTATGTTTAAAGGCCTGACAATGGCGGACGATCTAACTGCAGTCATTAAAAAGATGTTAGATTCCTCATCCGGCCAAGGATTGAAGTCATATGAGGCAATTTGCATAGCCAATCACATTGATTACGAAAAATGGAATAACCACCAAAGGAAGTTATCAAACGGCCCAGTGTTCCGAGTTATGGGCCAGTTCTTAGGTTATCCATCCTTAATCGAGAGAACTCATGAATTTTTTGAGAAAAGTCTTATATACTACAATGGAAGACCAGACTTGATGCGTGTTCACAACAACACACTGATCAATTCAACCTCCCAACGAGTTTGTTGGCAAGGACAAGAGGGTGGACTGGAAGGTCTACGGCAAAAAGGATGGACTATCCTCAATCTACTGGTTATTCAAAGAGAGGCTAAAATCAGAAACACTGCTGTCAAAGTCTTGGCACAAGGTGATAATCAAGTTATTTGCACACAGTATAAAACGAAGAAATCGAGAAACGTTGTAGAATTACAGGGTGCTCTCAATCAAATGGTTTCTAATAATGAGAAAATTATGACTGCAATCAAAATAGGGACAGGGAAGTTAGGACTTTTGATAAATGACGATGAGACTATGCAATCTGCAGATTACTTGAATTATGGAAAAATACCGATTTTCCGTGGAGTGATTAGAGGGTTAGAGACCAAGAGATGGTCACGAGTGACTTGTGTCACCAATGACCAAATACCCACTTGTGCTAATATAATGAGCTCAGTTTCCACAAATGCTCTCACCGTAGCTCATTTTGCTGAGAACCCAATCAATGCCATGATACAGTACAATTATTTTGGGACATTTGCTAGACTCTTGTTGATGATGCATGATCCTGCTCTTCGTCAATCATTGTATGAAGTTCAAGATAAGATACCGGGCTTGCACAGTTCTACTTTCAAATACGCCATGTTGTATTTGGACCCTTCCATTGGAGGAGTGTCGGGCATGTCTTTGTCCAGGTTTTTGATTAGAGCCTTCCCAGATCCCGTAACAGAAAGTCTCTCATTCTGGAGATTCATCCATGTACATGCTCGAAGTGAGCATCTGAAGGAGATGAGTGCAGTATTTGGAAACCCCGAGATAGCCAAGTTTCGAATAACTCACATAGACAAGCTAGTAGAAGATCCAACCTCTCTGAACATCGCTATGGGAATGAGTCCAGCGAACTTGTTAAAGACTGAGGTTAAAAAATGCTTAATCGAATCAAGACAAACCATCAGGAACCAGGTGATTAAGGATGCAACCATATATTTGTATCATGAAGAGGATCGGCTCAGAAGTTTCTTATGGTCAATAAATCCTCTGTTCCCTAGATTTTTAAGTGAATTCAAATCAGGCACTTTTTTGGGAGTCGCAGACGGGCTCATCAGTCTATTTCAAAATTCTCGTACTATTCGGAACTCCTTTAAGAAAAAGTATCATAGGGAATTGGATGATTTGATTGTGAGGAGTGAGGTATCCTCTTTGACACATTTAGGGAAACTTCATTTGAGAAGGGGATCATGTAAAATGTGGACATGTTCAGCTACTCATGCTGACACATTAAGATACAAATCCTGGGGCCGTACAGTTATTGGGACAACTGTACCCCATCCATTAGAAATGTTGGGTCCACAACATCGAAAAGAGACTCCTTGTGCACCATGTAACACATCAGGGTTCAATTATGTTTCTGTGCATTGTCCAGACGGGATCCATGACGTCTTTAGTTCACGGGGACCATTGCCTGCTTATCTAGGGTCTAAAACATCTGAATCTACATCTATTTTGCAGCCTTGGGAAAGGGAAAGCAAAGTCCCACTGATTAAAAGAGCTACACGTCTTAGAGATGCTATCTCTTGGTTTGTTGAACCCGACTCTAAACTAGCAATGACTATACTTTCTAACATCCACTCTTTAACAGGCGAAGAATGGACCAAAAGGCAGCATGGGTTCAAAAGAACAGGGTCTGCCCTTCATAGGTTTTCGACATCTCGGATGAGCCATGGTGGGTTCGCATCTCAGAGCACTGCAGCATTGACCAGGTTGATGGCAACTACAGACACCATGAGGGATCTGGGAGATCAGAATTTCGACTTTTTATTCCAAGCAACGTTGCTCTATGCTCAAATTACCACCACTGTTGCAAGAGACGGATGGATCACCAGTTGTACAGATCATTATCATATTGCCTGTAAGTCCTGTTTGAGACCCATAGAAGAGATCACCCTGGACTCAAGTATGGACTACACGCCCCCAGATGTATCCCATGTGCTGAAGACATGGAGGAATGGGGAAGGTTCGTGGGGACAAGAGATAAAACAGATCTATCCTTTAGAAGGGAATTGGAAGAATTTAGCACCTGCTGAGCAATCCTATCAAGTCGGCAGATGTATAGGTTTTCTATATGGAGACTTGGCGTATAGAAAATCTACTCATGCCGAGGACAGTTCTCTATTTCCTCTATCTATACAAGGTCGTATTAGAGGTCGAGGTTTCTTAAAAGGGTTGCTAGACGGATTAATGAGAGCAAGTTGCTGCCAAGTAATACACCGGAGAAGTCTGGCTCATTTGAAGAGGCCGGCCAACGCAGTGTACGGAGGTTTGATTTACTTGATTGATAAATTGAGTGTATCACCTCCATTCCTTTCTCTTACTAGATCAGGACCTATTAGAGACGAATTAGAAACGATTCCCCACAAGATCCCAACCTCCTATCCGACAAGCAACCGTGATATGGGGGTGATTGTCAGAAATTACTTCAAATACCAATGCCGTCTAATTGAAAAGGGAAAATACAGATCACATTATTCACAATTATGGTTATTCTCAGATGTCTTATCCATAGACTTCATTGGACCATTCTCTATTTCCACCACCCTCTTGCAAATCCTATACAAGCCATTTTTATCTGGGAAAGATAAGAATGAGTTGAGAGAGCTGGCAAATCTTTCTTCATTGCTAAGATCAGGAGAGGGGTGGGAAGACATACATGTGAAATTCTTCACCAAGGACATATTATTGTGTCCAGAGGAAATCAGACATGCTTGCAAGTTCGGGATTGCTAAGGATAATAATAAAGACATGAGCTATCCCCCTTGGGGAAGGGAATCCAGAGGGACAATTACAACAATCCCTGTTTATTATACGACCACCCCTTACCCAAAGATGCTAGAGATGCCTCCAAGAATCCAAAATCCCCTGCTGTCCGGAATCAGGTTGGGCCAATTACCAACTGGCGCTCATTATAAAATTCGGAGTATATTACATGGAATGGGAATCCATTACAGGGACTTCTTGAGTTGTGGAGACGGCTCCGGAGGGATGACTGCTGCATTACTACGAGAAAATGTGCATAGCAGAGGAATATTCAATAGTCTGTTAGAATTATCAGGGTCAGTCATGCGAGGCGCCTCTCCTGAGCCCCCCAGTGCCCTAGAAACTTTAGGAGGAGATAAATCGAGATGTGTAAATGGTGAAACATGTTGGGAATATCCATCTGACTTATGTGACCCAAGGACTTGGGACTATTTCCTCCGACTCAAAGCAGGCTTGGGGCTTCAAATTGATTTAATTGTAATGGATATGGAAGTTCGGGATTCTTCTACTAGCCTGAAAATTGAGACGAATGTTAGAAATTATGTGCACCGGATTTTGGATGAGCAAGGAGTTTTAATCTACAAGACTTATGGAACATATATTTGTGAGAGCGAAAAGAATGCAGTAACAATCCTTGGTCCCATGTTCAAGACGGTCGACTTAGTTCAAACAGAATTTAGTAGTTCTCAAACGTCTGAAGTATATATGGTATGTAAAGGTTTGAAGAAATTAATCGATGAACCCAATCCCGATTGGTCTTCCATCAATGAATCCTGGAAAAACCTGTACGCATTCCAGTCATCAGAACAGGAATTTGCCAGAGCAAAGAAGGTTAGTACATACTTTACCTTGACAGGTATTCCCTCCCAATTCATTCCTGATCCTTTTGTAAACATTGAGACTATGCTACAAATATTCGGAGTACCCACGGGTGTGTCTCATGCGGCTGCCTTAAAATCATCTGATAGACCTGCAGATTTATTGACCATTAGCCTTTTTTATATGGCGATTATATCGTATTATAACATCAATCATATCAGAGTAGGACCGATACCTCCGAACCCCCCATCAGATGGAATTGCACAAAATGTGGGGATCGCTATAACTGGTATAAGCTTTTGGCTGAGTTTGATGGAGAAAGACATTCCACTATATCAACAGTGTTTAGCAGTTATCCAGCAATCATTCCCGATTAGGTGGGAGGCTGTTTCAGTAAAAGGAGGATACAAGCAGAAGTGGAGTACTAGAGGTGATGGGCTCCCAAAAGATACCCGAACTTCAGACTCCTTGGCCCCAATCGGGAACTGGATCAGATCTCTGGAATTGGTCCGAAACCAAGTTCGTCTAAATCCATTCAATGAGATCTTGTTCAATCAGCTATGTCGTACAGTGGATAATCATTTGAAATGGTCAAATTTGCGAAGAAACACAGGAATGATTGAATGGATCAATAGACGAATTTCAAAAGAAGACCGGTCTATACTGATGTTGAAGAGTGACCTACACGAGGAAAACTCTTGGAGAGATTAAAAAATCATGAGGAGACTCCAAACTTTAAGTATGAAAAAAACTTTGATCCTTAAGACCCTCTTGTGGTTTTTATTTTTTATCTGGTTTTGTGGTCTTCGT。
在其他实施方式中，核酸包括SEQ ID NO：21的序列，如下所示(VSV载体：ConvacV2 South Africa)：
ACGAAGACAAACAAACCATTATTATCATTAAAAGGCTCAGGAGAAACTTTAACAGTAATCAAAATGTCTGTTACAGTCAAGAGAATCATTGACAACACAGTCATAGTTCCAAAACTTCCTGCAAATGAGGATCCAGTGGAATACCCGGCAGATTACTTCAGAAAATCAAAGGAGATTCCTCTTTACATCAATACTACAAAAAGTTTGTCAGATCTAAGAGGATATGTCTACCAAGGCCTCAAATCCGGAAATGTATCAATCATACATGTCAACAGCTACTTGTATGGAGCATTAAAGGACATCCGGGGTAAGTTGGATAAAGATTGGTCAAGTTTCGGAATAAACATCGGGAAAGCAGGGGATACAATCGGAATATTTGACCTTGTATCCTTGAAAGCCCTGGACGGCGTACTTCCAGATGGAGTATCGGATGCTTCCAGAACCAGCGCAGATGACAAATGGTTGCCTTTGTATCTACTTGGCTTATACAGAGTGGGCAGAACACAAATGCCTGAATACAGAAAAAAGCTCATGGATGGGCTGACAAATCAATGCAAAATGATCAATGAACAGTTTGAACCTCTTGTGCCAGAAGGTCGTGACATTTTTGATGTGTGGGGAAATGACAGTAATTACACAAAAATTGTCGCTGCAGTGGACATGTTCTTCCACATGTTCAAAAAACATGAATGTGCCTCGTTCAGATACGGAACTATTGTTTCCAGATTCAAAGATTGTGCTGCATTGGCAACATTTGGACACCTCTGCAAAATAACCGGAATGTCTACAGAAGATGTAACGACCTGGATCTTGAACCGAGAAGTTGCAGATGAAATGGTCCAAATGATGCTTCCAGGCCAAGAAATTGACAAGGCCGATTCATACATGCCTTATTTGATCGACTTTGGATTGTCTTCTAAGTCTCCATATTCTTCCGTCAAAAACCCTGCCTTCCACTTCTGGGGGCAATTGACAGCTCTTCTGCTCAGATCCACCAGAGCAAGGAATGCCCGACAGCCTGATGACATTGAGTATACATCTCTTACTACAGCAGGTTTGTTGTACGCTTATGCAGTAGGATCCTCTGCCGACTTGGCACAACAGTTTTGTGTTGGAGATAACAAATACACTCCAGATGATAGTACCGGAGGATTGACGACTAATGCACCGCCACAAGGCAGAGATGTGGTCGAATGGCTCGGATGGTTTGAAGATCAAAACAGAAAACCGACTCCTGATATGATGCAGTATGCGAAAAGAGCAGTCATGTCACTGCAAGGCCTAAGAGAGAAGACAATTGGCAAGTATGCTAAGTCAGAATTTGACAAATGACCCTATAATTCTCAGATCACCTATTATATATTATGCTACATATGAAAAAAACTAACAGATATCATGGATAATCTCACAAAAGTTCGTGAGTATCTCAAGTCCTATTCTCGTCTGGATCAGGCGGTAGGAGAGATAGATGAGATCGAAGCACAACGAGCTGAAAAGTCCAATTATGAGTTGTTCCAAGAGGATGGAGTGGAAGAGCATACTAAGCCCTCTTATTTTCAGGCAGCAGATGATTCTGACACAGAATCTGAACCAGAAATTGAAGACAATCAAGGTTTGTATGCACCAGATCCAGAAGCTGAGCAAGTTGAAGGCTTTATACAGGGGCCTTTAGATGACTATGCAGATGAGGAAGTGGATGTTGTATTTACTTCGGACTGGAAACAGCCTGAGCTTGAATCTGACGAGCATGGAAAGACCTTACGGTTGACATCGCCAGAGGGTTTAAGTGGAGAGCAGAAATCCCAGTGGCTTTCGACGATTAAAGCAGTCGTGCAAAGTGCCAAATACTGGAATCTGGCAGAGTGCACATTTGAAGCATCGGGAGAAGGGGTCATTATGAAGGAGCGCCAGATAACTCCGGATGTATATAAGGTCACTCCAGTGATGAACACACATCCGTCCCAATCAGAAGCAGTATCAGATGTTTGGTCTCTCTCAAAGACATCCATGACTTTCCAACCCAAGAAAGCAAGTCTTCAGCCTCTCACCATATCCTTGGATGAATTGTTCTCATCTAGAGGAGAGTTCATCTCTGTCGGAGGTGACGGACGAATGTCTCATAAAGAGGCCATCCTGCTCGGCCTGAGATACAAAAAGTTGTACAATCAGGCGAGAGTCAAATATTCTCTGTAGACTATGAAAAAAAGTAACAGATATCACGATCTAAGTGTTATCCCAATCCATTCATCATGAGTTCCTTAAAGAAGATTCTCGGTCTGAAGGGGAAAGGTAAGAAATCTAAGAAATTAGGGATCGCACCACCCCCTTATGAAGAGGACACTAGCATGGAGTATGCTCCGAGCGCTCCAATTGACAAATCCTATTTTGGAGTTGACGAGATGGACACCTATGATCCGAATCAATTAAGATATGAGAAATTCTTCTTTACAGTGAAAATGACGGTTAGATCTAATCGTCCGTTCAGAACATACTCAGATGTGGCAGCCGCTGTATCCCATTGGGATCACATGTACATCGGAATGGCAGGGAAACGTCCCTTCTACAAAATCTTGGCTTTTTTGGGTTCTTCTAATCTAAAGGCCACTCCAGCGGTATTGGCAGATCAAGGTCAACCAGAGTATCACACTCACTGCGAAGGCAGGGCTTATTTGCCACATAGGATGGGGAAGACCCCTCCCATGCTCAATGTACCAGAGCACTTCAGAAGACCATTCAATATAGGTCTTTACAAGGGAACGATTGAGCTCACAATGACCATCTACGATGATGAGTCACTGGAAGCAGCTCCTATGATCTGGGATCATTTCAATTCTTCCAAATTTTCTGATTTCAGAGAGAAGGCCTTAATGTTTGGCCTGATTGTCGAGAAAAAGGCATCTGGAGCGTGGGTCCTGGATTCTATCAGCCACTTCAAATGAGCTAGTCTAACTTCTAGCTTCTGAACAATCCCCGGTTTACTCAGTCTCTCCTAATTCCAGCCTCTCGAACAACTAATATCCTGTCTTTTCTATCCCTATGAAAAAAACTAACAGAGATCGATCTGTTTACGCGTCACTATGAAGTGCCTTTTGTACTTAGCCTTTTTATTCATTGGGGTGAATTGCAAGTTCACCATAGTTTTTCCACACAACCAAAAAGGAAACTGGAAAAATGTTCCTTCTAATTACCATTATTGCCCGTCAAGCTCAGATTTAAATTGGCATAATGACTTAATAGGCACAGCCATACAAGTCAAAATGCCCAAGAGTCACAAGGCTATTCAAGCAGACGGTTGGATGTGTCATGCTTCCAAATGGGTCACTACTTGTGATTTCCGCTGGTATGGACCGAAGTATATAACACAGTCCATCCGATCCTTCACTCCATCTGTAGAACAATGCAAGGAAAGCATTGAACAAACGAAACAAGGAACTTGGCTGAATCCAGGCTTCCCTCCTCAAAGTTGTGGATATGCAACTGTGACGGATGCCGAAGCAGTGATTGTCCAGGTGACTCCTCACCATGTGCTGGTTGATGAATACACAGGAGAATGGGTTGATTCACAGTTCATCAACGGAAAATGCAGCAATTACATATGCCCCACTGTCCATAACTCTACAACCTGGCATTCTGACTATAAGGTCAAAGGGCTATGTGATTCTAACCTCATTTCCATGGACATCACCTTCTTCTCAGAGGACGGAGAGCTATCATCCCTGGGAAAGGAGGGCACAGGGTTCAGAAGTAACTACTTTGCTTATGAAACTGGAGGCAAGGCCTGCAAAATGCAATACTGCAAGCATTGGGGAGTCAGACTCCCATCAGGTGTCTGGTTCGAGATGGCTGATAAGGATCTCTTTGCTGCAGCCAGATTCCCTGAATGCCCAGAAGGGTCAAGTATCTCTGCTCCATCTCAGACCTCAGTGGATGTAAGTCTAATTCAGGACGTTGAGAGGATCTTGGATTATTCCCTCTGCCAAGAAACCTGGAGCAAAATCAGAGCGGGTCTTCCAATCTCTCCAGTGGATCTCAGCTATCTTGCTCCTAAAAACCCAGGAACCGGTCCTGCTTTCACCATAATCAATGGTACCCTAAAATACTTTGAGACCAGATACATCAGAGTCGATATTGCTGCTCCAATCCTCTCAAGAATGGTCGGAATGATCAGTGGAACTACCACAGAAAGGGAACTGTGGGATGACTGGGCACCATATGAAGACGTGGAAATTGGACCCAATGGAGTTCTGAGGACCAGTTCAGGATATAAGTTTCCTTTATACATGATTGGACATGGTATGTTGGACTCCGATCTTCATCTTAGCTCAAAGGCTCAGGTGTTCGAACATCCTCACATTCAAGACGCTGCTTCGCAACTTCCTGATGATGAGAGTTTATTTTTTGGTGATACTGGGCTATCCAAAAATCCAATCGAGCTTGTAGAAGGTTGGTTCAGTAGTTGGAAAAGCTCTATTGCCTCTTTTTTCTTTATCATAGGGTTAATCATTGGACTATTCTTGGTTCTCCGAGTTGGTATCCATCTTTGCATTAAATTAAAGCACACCAAGAAAAGACAGATTTATACAGACATAGAGATGAACCGACTTGGAAAGTAACTCAAATCCTGCTAGGTATGAAAAAAACTAACaGATATCACGCTCGAGCGTACGCCACCATGTTCGTGTTTCTGGTGCTGCTGCCTCTGGTGAGCTCCCAGTGCGTGAACTTCACCACAAGGACCCAGCTGCCCCCTGCCTATACCAATTCCTTCACACGGGGCGTGTACTATCCCGACAAGGTGTTCCGGAGCAGCGTGCTGCACTCCACACAGGATCTGTTTCTGCCTTTCTTTTCTAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAATGGCACAAAGCGGTTCGCCAATCCAGTGCTGCCCTTTAACGATGGCGTGTACTTCGCCTCCACCGAGAAGTCTAACATCATCAGAGGCTGGATCTTTGGCACCACACTGGACAGCAAGACACAGTCCCTGCTGATCGTGAACAATGCCACCAACGTGGTCATCAAGGTGTGCGAGTTCCAGTTTTGTAATGATCCATTCCTGGGCGTGTACTATCACAAGAACAATAAGTCTTGGATGGAGAGCGAGTTTCGCGTGTATTCCTCTGCCAACAATTGCACATTTGAGTACGTGTCCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAATTTCAAGAACCTGAGGGAGTTCGTGTTTAAGAATATCGATGGCTACTTCAAAATCTACTCCAAGCACACCCCAATCAACCTGGTGCGCGGCCTGCCACAGGGCTTCTCTGCCCTGGAGCCACTGGTGGATCTGCCCATCGGCATCAACATCACCCGGTTTCAGACACTGCTGGCCCTGCACAGAAGCTACCTGACACCAGGCGACAGCTCCTCTGGATGGACCGCAGGAGCAGCAGCCTACTATGTGGGCTATCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAATGGCACCATCACAGACGCCGTGGATTGCGCCCTGGATCCCCTGTCTGAGACCAAGTGTACACTGAAGAGCTTTACCGTGGAGAAGGGCATCTATCAGACAAGCAATTTCAGGGTGCAGCCTACCGAGTCCATCGTGCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAATATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGAAGGGCTTTAACTGTTATTTCCCTCTGCAGAGCTACGGCTTCCAGCCAACATATGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACCTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGACTGACCGGAACAGGCGTGCTGACCGAGTCCAACAAGAAGTTCCTGCCTTTTCAGCAGTTCGGCAGGGACATCGCAGATACCACAGACGCCGTGCGCGACCCTCAGACCCTGGAGATCCTGGACATCACACCATGCTCCTTCGGCGGCGTGTCTGTGATCACACCAGGCACCAATACAAGCAACCAGGTGGCCGTGCTGTATCAGGGCGTGAATTGTACCGAGGTGCCAGTGGCAATCCACGCAGATCAGCTGACCCCTACATGGCGGGTGTACTCTACCGGCAGCAACGTGTTCCAGACAAGAGCCGGATGCCTGATCGGAGCAGAGCACGTGAACAATAGCTATGAGTGCGACATCCCTATCGGCGCCGGCATCTGTGCCTCCTACCAGACCCAGACAAACTCCCCAGAATCAAGCGTGATTCCTCTGGTCCATCCACTGGCAGATCCCTCCACAGTGTTCAAAGACGGAGATGAGGCCGAAGACTTTGTGGAAGTCCACCTGCCTGATGTGCATAACCAGGTGTCTGGCGTCGACCTGGGACTGCCAAATTGGGGCAAGTACGTGCTGCTGAGTGCTGGAGCACTGACTGCCCTGATGCTGATCATTTTCCTGATGACCTGCTGTCGGCGCGTGAACAGAAGTGAGCCCACTCAGCACAATCTGCGAGGAACCGGGAGAGAAGTGTCAGTCACACCTCAGAGCGGGAAAATCATTAGTAGTTGGGAATCACATAAAAGCGGGGGCGAGACCAGGCTGTGAGCTAGCCAGATTCTTCATGTTTGGACCAAATCAACTTGTGATACCATGCTCAAAGAGGCCTCAATTATATTTGAGTTTTTAATTTTTATGAAAAAAACTAACAGCAATCATGGAAGTCCACGATTTTGAGACCGACGAGTTCAATGATTTCAATGAAGATGACTATGCCACAAGAGAATTCCTGAATCCCGATGAGCGCATGACGTACTTGAATCATGCTGATTACAATTTGAATTCTCCTCTAATTAGTGATGATATTGACAATTTGATCAGGAAATTCAATTCTCTTCCGATTCCCTCGATGTGGGATAGTAAGAACTGGGATGGAGTTCTTGAGATGTTAACATCATGTCAAGCCAATCCCATCTCAACATCTCAGATGCATAAATGGATGGGAAGTTGGTTAATGTCTGATAATCATGATGCCAGTCAAGGGTATAGTTTTTTACATGAAGTGGACAAAGAGGCAGAAATAACATTTGACGTGGTGGAGACCTTCATCCGCGGCTGGGGCAACAAACCAATTGAATACATCAAAAAGGAAAGATGGACTGACTCATTCAAAATTCTCGCTTATTTGTGTCAAAAGTTTTTGGACTTACACAAGTTGACATTAATCTTAAATGCTGTCTCTGAGGTGGAATTGCTCAACTTGGCGAGGACTTTCAAAGGCAAAGTCAGAAGAAGTTCTCATGGAACGAACATATGCAGGATTAGGGTTCCCAGCTTGGGTCCTACTTTTATTTCAGAAGGATGGGCTTACTTCAAGAAACTTGATATTCTAATGGACCGAAACTTTCTGTTAATGGTCAAAGATGTGATTATAGGGAGGATGCAAACGGTGCTATCCATGGTATGTAGAATAGACAACCTGTTCTCAGAGCAAGACATCTTCTCCCTTCTAAATATCTACAGAATTGGAGATAAAATTGTGGAGAGGCAGGGAAATTTTTCTTATGACTTGATTAAAATGGTGGAACCGATATGCAACTTGAAGCTGATGAAATTAGCAAGAGAATCAAGGCCTTTAGTCCCACAATTCCCTCATTTTGAAAATCATATCAAGACTTCTGTTGATGAAGGGGCAAAAATTGACCGAGGTATAAGATTCCTCCATGATCAGATAATGAGTGTGAAAACAGTGGATCTCACACTGGTGATTTATGGATCGTTCAGACATTGGGGTCATCCTTTTATAGATTATTACACTGGACTAGAAAAATTACATTCCCAAGTAACCATGAAGAAAGATATTGATGTGTCATATGCAAAAGCACTTGCAAGTGATTTAGCTCGGATTGTTCTATTTCAACAGTTCAATGATCATAAAAAGTGGTTCGTGAATGGAGACTTGCTCCCTCATGATCATCCCTTTAAAAGTCATGTTAAAGAAAATACATGGCCCACAGCTGCTCAAGTTCAAGATTTTGGAGATAAATGGCATGAACTTCCGCTGATTAAATGTTTTGAAATACCCGACTTACTAGACCCATCGATAATATACTCTGACAAAAGTCATTCAATGAATAGGTCAGAGGTGTTGAAACATGTCCGAATGAATCCGAACACTCCTATCCCTAGTAAAAAGGTGTTGCAGACTATGTTGGACACAAAGGCTACCAATTGGAAAGAATTTCTTAAAGAGATTGATGAGAAGGGCTTAGATGATGATGATCTAATTATTGGTCTTAAAGGAAAGGAGAGGGAACTGAAGTTGGCAGGTAGATTTTTCTCCCTAATGTCTTGGAAATTGCGAGAATACTTTGTAATTACCGAATATTTGATAAAGACTCATTTCGTCCCTATGTTTAAAGGCCTGACAATGGCGGACGATCTAACTGCAGTCATTAAAAAGATGTTAGATTCCTCATCCGGCCAAGGATTGAAGTCATATGAGGCAATTTGCATAGCCAATCACATTGATTACGAAAAATGGAATAACCACCAAAGGAAGTTATCAAACGGCCCAGTGTTCCGAGTTATGGGCCAGTTCTTAGGTTATCCATCCTTAATCGAGAGAACTCATGAATTTTTTGAGAAAAGTCTTATATACTACAATGGAAGACCAGACTTGATGCGTGTTCACAACAACACACTGATCAATTCAACCTCCCAACGAGTTTGTTGGCAAGGACAAGAGGGTGGACTGGAAGGTCTACGGCAAAAAGGATGGACTATCCTCAATCTACTGGTTATTCAAAGAGAGGCTAAAATCAGAAACACTGCTGTCAAAGTCTTGGCACAAGGTGATAATCAAGTTATTTGCACACAGTATAAAACGAAGAAATCGAGAAACGTTGTAGAATTACAGGGTGCTCTCAATCAAATGGTTTCTAATAATGAGAAAATTATGACTGCAATCAAAATAGGGACAGGGAAGTTAGGACTTTTGATAAATGACGATGAGACTATGCAATCTGCAGATTACTTGAATTATGGAAAAATACCGATTTTCCGTGGAGTGATTAGAGGGTTAGAGACCAAGAGATGGTCACGAGTGACTTGTGTCACCAATGACCAAATACCCACTTGTGCTAATATAATGAGCTCAGTTTCCACAAATGCTCTCACCGTAGCTCATTTTGCTGAGAACCCAATCAATGCCATGATACAGTACAATTATTTTGGGACATTTGCTAGACTCTTGTTGATGATGCATGATCCTGCTCTTCGTCAATCATTGTATGAAGTTCAAGATAAGATACCGGGCTTGCACAGTTCTACTTTCAAATACGCCATGTTGTATTTGGACCCTTCCATTGGAGGAGTGTCGGGCATGTCTTTGTCCAGGTTTTTGATTAGAGCCTTCCCAGATCCCGTAACAGAAAGTCTCTCATTCTGGAGATTCATCCATGTACATGCTCGAAGTGAGCATCTGAAGGAGATGAGTGCAGTATTTGGAAACCCCGAGATAGCCAAGTTTCGAATAACTCACATAGACAAGCTAGTAGAAGATCCAACCTCTCTGAACATCGCTATGGGAATGAGTCCAGCGAACTTGTTAAAGACTGAGGTTAAAAAATGCTTAATCGAATCAAGACAAACCATCAGGAACCAGGTGATTAAGGATGCAACCATATATTTGTATCATGAAGAGGATCGGCTCAGAAGTTTCTTATGGTCAATAAATCCTCTGTTCCCTAGATTTTTAAGTGAATTCAAATCAGGCACTTTTTTGGGAGTCGCAGACGGGCTCATCAGTCTATTTCAAAATTCTCGTACTATTCGGAACTCCTTTAAGAAAAAGTATCATAGGGAATTGGATGATTTGATTGTGAGGAGTGAGGTATCCTCTTTGACACATTTAGGGAAACTTCATTTGAGAAGGGGATCATGTAAAATGTGGACATGTTCAGCTACTCATGCTGACACATTAAGATACAAATCCTGGGGCCGTACAGTTATTGGGACAACTGTACCCCATCCATTAGAAATGTTGGGTCCACAACATCGAAAAGAGACTCCTTGTGCACCATGTAACACATCAGGGTTCAATTATGTTTCTGTGCATTGTCCAGACGGGATCCATGACGTCTTTAGTTCACGGGGACCATTGCCTGCTTATCTAGGGTCTAAAACATCTGAATCTACATCTATTTTGCAGCCTTGGGAAAGGGAAAGCAAAGTCCCACTGATTAAAAGAGCTACACGTCTTAGAGATGCTATCTCTTGGTTTGTTGAACCCGACTCTAAACTAGCAATGACTATACTTTCTAACATCCACTCTTTAACAGGCGAAGAATGGACCAAAAGGCAGCATGGGTTCAAAAGAACAGGGTCTGCCCTTCATAGGTTTTCGACATCTCGGATGAGCCATGGTGGGTTCGCATCTCAGAGCACTGCAGCATTGACCAGGTTGATGGCAACTACAGACACCATGAGGGATCTGGGAGATCAGAATTTCGACTTTTTATTCCAAGCAACGTTGCTCTATGCTCAAATTACCACCACTGTTGCAAGAGACGGATGGATCACCAGTTGTACAGATCATTATCATATTGCCTGTAAGTCCTGTTTGAGACCCATAGAAGAGATCACCCTGGACTCAAGTATGGACTACACGCCCCCAGATGTATCCCATGTGCTGAAGACATGGAGGAATGGGGAAGGTTCGTGGGGACAAGAGATAAAACAGATCTATCCTTTAGAAGGGAATTGGAAGAATTTAGCACCTGCTGAGCAATCCTATCAAGTCGGCAGATGTATAGGTTTTCTATATGGAGACTTGGCGTATAGAAAATCTACTCATGCCGAGGACAGTTCTCTATTTCCTCTATCTATACAAGGTCGTATTAGAGGTCGAGGTTTCTTAAAAGGGTTGCTAGACGGATTAATGAGAGCAAGTTGCTGCCAAGTAATACACCGGAGAAGTCTGGCTCATTTGAAGAGGCCGGCCAACGCAGTGTACGGAGGTTTGATTTACTTGATTGATAAATTGAGTGTATCACCTCCATTCCTTTCTCTTACTAGATCAGGACCTATTAGAGACGAATTAGAAACGATTCCCCACAAGATCCCAACCTCCTATCCGACAAGCAACCGTGATATGGGGGTGATTGTCAGAAATTACTTCAAATACCAATGCCGTCTAATTGAAAAGGGAAAATACAGATCACATTATTCACAATTATGGTTATTCTCAGATGTCTTATCCATAGACTTCATTGGACCATTCTCTATTTCCACCACCCTCTTGCAAATCCTATACAAGCCATTTTTATCTGGGAAAGATAAGAATGAGTTGAGAGAGCTGGCAAATCTTTCTTCATTGCTAAGATCAGGAGAGGGGTGGGAAGACATACATGTGAAATTCTTCACCAAGGACATATTATTGTGTCCAGAGGAAATCAGACATGCTTGCAAGTTCGGGATTGCTAAGGATAATAATAAAGACATGAGCTATCCCCCTTGGGGAAGGGAATCCAGAGGGACAATTACAACAATCCCTGTTTATTATACGACCACCCCTTACCCAAAGATGCTAGAGATGCCTCCAAGAATCCAAAATCCCCTGCTGTCCGGAATCAGGTTGGGCCAATTACCAACTGGCGCTCATTATAAAATTCGGAGTATATTACATGGAATGGGAATCCATTACAGGGACTTCTTGAGTTGTGGAGACGGCTCCGGAGGGATGACTGCTGCATTACTACGAGAAAATGTGCATAGCAGAGGAATATTCAATAGTCTGTTAGAATTATCAGGGTCAGTCATGCGAGGCGCCTCTCCTGAGCCCCCCAGTGCCCTAGAAACTTTAGGAGGAGATAAATCGAGATGTGTAAATGGTGAAACATGTTGGGAATATCCATCTGACTTATGTGACCCAAGGACTTGGGACTATTTCCTCCGACTCAAAGCAGGCTTGGGGCTTCAAATTGATTTAATTGTAATGGATATGGAAGTTCGGGATTCTTCTACTAGCCTGAAAATTGAGACGAATGTTAGAAATTATGTGCACCGGATTTTGGATGAGCAAGGAGTTTTAATCTACAAGACTTATGGAACATATATTTGTGAGAGCGAAAAGAATGCAGTAACAATCCTTGGTCCCATGTTCAAGACGGTCGACTTAGTTCAAACAGAATTTAGTAGTTCTCAAACGTCTGAAGTATATATGGTATGTAAAGGTTTGAAGAAATTAATCGATGAACCCAATCCCGATTGGTCTTCCATCAATGAATCCTGGAAAAACCTGTACGCATTCCAGTCATCAGAACAGGAATTTGCCAGAGCAAAGAAGGTTAGTACATACTTTACCTTGACAGGTATTCCCTCCCAATTCATTCCTGATCCTTTTGTAAACATTGAGACTATGCTACAAATATTCGGAGTACCCACGGGTGTGTCTCATGCGGCTGCCTTAAAATCATCTGATAGACCTGCAGATTTATTGACCATTAGCCTTTTTTATATGGCGATTATATCGTATTATAACATCAATCATATCAGAGTAGGACCGATACCTCCGAACCCCCCATCAGATGGAATTGCACAAAATGTGGGGATCGCTATAACTGGTATAAGCTTTTGGCTGAGTTTGATGGAGAAAGACATTCCACTATATCAACAGTGTTTAGCAGTTATCCAGCAATCATTCCCGATTAGGTGGGAGGCTGTTTCAGTAAAAGGAGGATACAAGCAGAAGTGGAGTACTAGAGGTGATGGGCTCCCAAAAGATACCCGAACTTCAGACTCCTTGGCCCCAATCGGGAACTGGATCAGATCTCTGGAATTGGTCCGAAACCAAGTTCGTCTAAATCCATTCAATGAGATCTTGTTCAATCAGCTATGTCGTACAGTGGATAATCATTTGAAATGGTCAAATTTGCGAAGAAACACAGGAATGATTGAATGGATCAATAGACGAATTTCAAAAGAAGACCGGTCTATACTGATGTTGAAGAGTGACCTACACGAGGAAAACTCTTGGAGAGATTAAAAAATCATGAGGAGACTCCAAACTTTAAGTATGAAAAAAACTTTGATCCTTAAGACCCTCTTGTGGTTTTTATTTTTTATCTGGTTTTGTGGTCTTCGT。
在一个实施方式中，核酸包括SEQ ID NO：22的序列，如下所示(VSV载体：ConvacV3 China)：
ACGAAGACAAACAAACCATTATTATCATTAAAAGGCTCAGGAGAAACTTTAACAGTAATCAAAATGTCTGTTACAGTCAAGAGAATCATTGACAACACAGTCATAGTTCCAAAACTTCCTGCAAATGAGGATCCAGTGGAATACCCGGCAGATTACTTCAGAAAATCAAAGGAGATTCCTCTTTACATCAATACTACAAAAAGTTTGTCAGATCTAAGAGGATATGTCTACCAAGGCCTCAAATCCGGAAATGTATCAATCATACATGTCAACAGCTACTTGTATGGAGCATTAAAGGACATCCGGGGTAAGTTGGATAAAGATTGGTCAAGTTTCGGAATAAACATCGGGAAAGCAGGGGATACAATCGGAATATTTGACCTTGTATCCTTGAAAGCCCTGGACGGCGTACTTCCAGATGGAGTATCGGATGCTTCCAGAACCAGCGCAGATGACAAATGGTTGCCTTTGTATCTACTTGGCTTATACAGAGTGGGCAGAACACAAATGCCTGAATACAGAAAAAAGCTCATGGATGGGCTGACAAATCAATGCAAAATGATCAATGAACAGTTTGAACCTCTTGTGCCAGAAGGTCGTGACATTTTTGATGTGTGGGGAAATGACAGTAATTACACAAAAATTGTCGCTGCAGTGGACATGTTCTTCCACATGTTCAAAAAACATGAATGTGCCTCGTTCAGATACGGAACTATTGTTTCCAGATTCAAAGATTGTGCTGCATTGGCAACATTTGGACACCTCTGCAAAATAACCGGAATGTCTACAGAAGATGTAACGACCTGGATCTTGAACCGAGAAGTTGCAGATGAAATGGTCCAAATGATGCTTCCAGGCCAAGAAATTGACAAGGCCGATTCATACATGCCTTATTTGATCGACTTTGGATTGTCTTCTAAGTCTCCATATTCTTCCGTCAAAAACCCTGCCTTCCACTTCTGGGGGCAATTGACAGCTCTTCTGCTCAGATCCACCAGAGCAAGGAATGCCCGACAGCCTGATGACATTGAGTATACATCTCTTACTACAGCAGGTTTGTTGTACGCTTATGCAGTAGGATCCTCTGCCGACTTGGCACAACAGTTTTGTGTTGGAGATAACAAATACACTCCAGATGATAGTACCGGAGGATTGACGACTAATGCACCGCCACAAGGCAGAGATGTGGTCGAATGGCTCGGATGGTTTGAAGATCAAAACAGAAAACCGACTCCTGATATGATGCAGTATGCGAAAAGAGCAGTCATGTCACTGCAAGGCCTAAGAGAGAAGACAATTGGCAAGTATGCTAAGTCAGAATTTGACAAATGACCCTATAATTCTCAGATCACCTATTATATATTATGCTACATATGAAAAAAACTAACAGATATCATGGATAATCTCACAAAAGTTCGTGAGTATCTCAAGTCCTATTCTCGTCTGGATCAGGCGGTAGGAGAGATAGATGAGATCGAAGCACAACGAGCTGAAAAGTCCAATTATGAGTTGTTCCAAGAGGATGGAGTGGAAGAGCATACTAAGCCCTCTTATTTTCAGGCAGCAGATGATTCTGACACAGAATCTGAACCAGAAATTGAAGACAATCAAGGTTTGTATGCACCAGATCCAGAAGCTGAGCAAGTTGAAGGCTTTATACAGGGGCCTTTAGATGACTATGCAGATGAGGAAGTGGATGTTGTATTTACTTCGGACTGGAAACAGCCTGAGCTTGAATCTGACGAGCATGGAAAGACCTTACGGTTGACATCGCCAGAGGGTTTAAGTGGAGAGCAGAAATCCCAGTGGCTTTCGACGATTAAAGCAGTCGTGCAAAGTGCCAAATACTGGAATCTGGCAGAGTGCACATTTGAAGCATCGGGAGAAGGGGTCATTATGAAGGAGCGCCAGATAACTCCGGATGTATATAAGGTCACTCCAGTGATGAACACACATCCGTCCCAATCAGAAGCAGTATCAGATGTTTGGTCTCTCTCAAAGACATCCATGACTTTCCAACCCAAGAAAGCAAGTCTTCAGCCTCTCACCATATCCTTGGATGAATTGTTCTCATCTAGAGGAGAGTTCATCTCTGTCGGAGGTGACGGACGAATGTCTCATAAAGAGGCCATCCTGCTCGGCCTGAGATACAAAAAGTTGTACAATCAGGCGAGAGTCAAATATTCTCTGTAGACTATGAAAAAAAGTAACAGATATCACGATCTAAGTGTTATCCCAATCCATTCATCATGAGTTCCTTAAAGAAGATTCTCGGTCTGAAGGGGAAAGGTAAGAAATCTAAGAAATTAGGGATCGCACCACCCCCTTATGAAGAGGACACTAGCATGGAGTATGCTCCGAGCGCTCCAATTGACAAATCCTATTTTGGAGTTGACGAGATGGACACCTATGATCCGAATCAATTAAGATATGAGAAATTCTTCTTTACAGTGAAAATGACGGTTAGATCTAATCGTCCGTTCAGAACATACTCAGATGTGGCAGCCGCTGTATCCCATTGGGATCACATGTACATCGGAATGGCAGGGAAACGTCCCTTCTACAAAATCTTGGCTTTTTTGGGTTCTTCTAATCTAAAGGCCACTCCAGCGGTATTGGCAGATCAAGGTCAACCAGAGTATCACACTCACTGCGAAGGCAGGGCTTATTTGCCACATAGGATGGGGAAGACCCCTCCCATGCTCAATGTACCAGAGCACTTCAGAAGACCATTCAATATAGGTCTTTACAAGGGAACGATTGAGCTCACAATGACCATCTACGATGATGAGTCACTGGAAGCAGCTCCTATGATCTGGGATCATTTCAATTCTTCCAAATTTTCTGATTTCAGAGAGAAGGCCTTAATGTTTGGCCTGATTGTCGAGAAAAAGGCATCTGGAGCGTGGGTCCTGGATTCTATCAGCCACTTCAAATGAGCTAGTCTAACTTCTAGCTTCTGAACAATCCCCGGTTTACTCAGTCTCTCCTAATTCCAGCCTCTCGAACAACTAATATCCTGTCTTTTCTATCCCTATGAAAAAAACTAACAGAGATCGATCTGTTTACGCGTCACTATGAAGTGCCTTTTGTACTTAGCCTTTTTATTCATTGGGGTGAATTGCAAGTTCACCATAGTTTTTCCACACAACCAAAAAGGAAACTGGAAAAATGTTCCTTCTAATTACCATTATTGCCCGTCAAGCTCAGATTTAAATTGGCATAATGACTTAATAGGCACAGCCATACAAGTCAAAATGCCCAAGAGTCACAAGGCTATTCAAGCAGACGGTTGGATGTGTCATGCTTCCAAATGGGTCACTACTTGTGATTTCCGCTGGTATGGACCGAAGTATATAACACAGTCCATCCGATCCTTCACTCCATCTGTAGAACAATGCAAGGAAAGCATTGAACAAACGAAACAAGGAACTTGGCTGAATCCAGGCTTCCCTCCTCAAAGTTGTGGATATGCAACTGTGACGGATGCCGAAGCAGTGATTGTCCAGGTGACTCCTCACCATGTGCTGGTTGATGAATACACAGGAGAATGGGTTGATTCACAGTTCATCAACGGAAAATGCAGCAATTACATATGCCCCACTGTCCATAACTCTACAACCTGGCATTCTGACTATAAGGTCAAAGGGCTATGTGATTCTAACCTCATTTCCATGGACATCACCTTCTTCTCAGAGGACGGAGAGCTATCATCCCTGGGAAAGGAGGGCACAGGGTTCAGAAGTAACTACTTTGCTTATGAAACTGGAGGCAAGGCCTGCAAAATGCAATACTGCAAGCATTGGGGAGTCAGACTCCCATCAGGTGTCTGGTTCGAGATGGCTGATAAGGATCTCTTTGCTGCAGCCAGATTCCCTGAATGCCCAGAAGGGTCAAGTATCTCTGCTCCATCTCAGACCTCAGTGGATGTAAGTCTAATTCAGGACGTTGAGAGGATCTTGGATTATTCCCTCTGCCAAGAAACCTGGAGCAAAATCAGAGCGGGTCTTCCAATCTCTCCAGTGGATCTCAGCTATCTTGCTCCTAAAAACCCAGGAACCGGTCCTGCTTTCACCATAATCAATGGTACCCTAAAATACTTTGAGACCAGATACATCAGAGTCGATATTGCTGCTCCAATCCTCTCAAGAATGGTCGGAATGATCAGTGGAACTACCACAGAAAGGGAACTGTGGGATGACTGGGCACCATATGAAGACGTGGAAATTGGACCCAATGGAGTTCTGAGGACCAGTTCAGGATATAAGTTTCCTTTATACATGATTGGACATGGTATGTTGGACTCCGATCTTCATCTTAGCTCAAAGGCTCAGGTGTTCGAACATCCTCACATTCAAGACGCTGCTTCGCAACTTCCTGATGATGAGAGTTTATTTTTTGGTGATACTGGGCTATCCAAAAATCCAATCGAGCTTGTAGAAGGTTGGTTCAGTAGTTGGAAAAGCTCTATTGCCTCTTTTTTCTTTATCATAGGGTTAATCATTGGACTATTCTTGGTTCTCCGAGTTGGTATCCATCTTTGCATTAAATTAAAGCACACCAAGAAAAGACAGATTTATACAGACATAGAGATGAACCGACTTGGAAAGTAACTCAAATCCTGCTAGGTATGAAAAAAACTAACaGATATCACGCtcgagGCCACCATGTTCGTGTTTCTGGTGCTGCTGCCTCTGGTGAGCTCCCAGTGCGTGAACCTGACCACAAGGACCCAGCTGCCCCCTGCCTATACCAATTCCTTCACACGGGGCGTGTACTATCCCGACAAGGTGTTCCGGAGCAGCGTGCTGCACTCCACACAGGATCTGTTTCTGCCTTTCTTTTCTAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAATGGCACAAAGCGGTTCGACAATCCAGTGCTGCCCTTTAACGATGGCGTGTACTTCGCCTCCACCGAGAAGTCTAACATCATCAGAGGCTGGATCTTTGGCACCACACTGGACAGCAAGACACAGTCCCTGCTGATCGTGAACAATGCCACCAACGTGGTCATCAAGGTGTGCGAGTTCCAGTTTTGTAATGATCCATTCCTGGGCGTGTACTATCACAAGAACAATAAGTCTTGGATGGAGAGCGAGTTTCGCGTGTATTCCTCTGCCAACAATTGCACATTTGAGTACGTGTCCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAATTTCAAGAACCTGAGGGAGTTCGTGTTTAAGAATATCGATGGCTACTTCAAAATCTACTCCAAGCACACCCCAATCAACCTGGTGCGCGACCTGCCACAGGGCTTCTCTGCCCTGGAGCCACTGGTGGATCTGCCCATCGGCATCAACATCACCCGGTTTCAGACACTGCTGGCCCTGCACAGAAGCTACCTGACACCAGGCGACAGCTCCTCTGGATGGACCGCAGGAGCAGCAGCCTACTATGTGGGCTATCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAATGGCACCATCACAGACGCCGTGGATTGCGCCCTGGATCCCCTGTCTGAGACCAAGTGTACACTGAAGAGCTTTACCGTGGAGAAGGGCATCTATCAGACAAGCAATTTCAGGGTGCAGCCTACCGAGTCCATCGTGCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAAGATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGGAGGGCTTTAACTGTTATTTCCCTCTGCAGAGCTACGGCTTCCAGCCAACAAACGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACCTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGACTGACCGGAACAGGCGTGCTGACCGAGTCCAACAAGAAGTTCCTGCCTTTTCAGCAGTTCGGCAGGGACATCGCAGATACCACAGACGCCGTGCGCGACCCTCAGACCCTGGAGATCCTGGACATCACACCATGCTCCTTCGGCGGCGTGTCTGTGATCACACCAGGCACCAATACAAGCAACCAGGTGGCCGTGCTGTATCAGGACGTGAATTGTACCGAGGTGCCAGTGGCAATCCACGCAGATCAGCTGACCCCTACATGGCGGGTGTACTCTACCGGCAGCAACGTGTTCCAGACAAGAGCCGGATGCCTGATCGGAGCAGAGCACGTGAACAATAGCTATGAGTGCGACATCCCTATCGGCGCCGGCATCTGTGCCTCCTACCAGACCCAGACAAACTCCCCAAGGTCTGGATCCGGCTACATCCCCGAGGCCCCCAGAGACGGCCAGGCCTACGTGCGGAAGGACGGCGAGTGGGTACTGctcagcaccttcctgggcAGCAGTTGGAAAAGCTCCATCGCCTCCTTTTTCTTTATCATCGGCCTGATCATCGGACTGTTCCTGGTGCTCCGCGTGGGTATCCACCTGTGCATCAAGCTGAAGCACACCAAGAAAAGACAGATTTATACAGACATCGAGATGAACCGACTTGGAAAGTAAGCTAGCCAGATTCTTCATGTTTGGACCAAATCAACTTGTGATACCATGCTCAAAGAGGCCTCAATTATATTTGAGTTTTTAATTTTTATGAAAAAAACTAACAGCAATCATGGAAGTCCACGATTTTGAGACCGACGAGTTCAATGATTTCAATGAAGATGACTATGCCACAAGAGAATTCCTGAATCCCGATGAGCGCATGACGTACTTGAATCATGCTGATTACAATTTGAATTCTCCTCTAATTAGTGATGATATTGACAATTTGATCAGGAAATTCAATTCTCTTCCGATTCCCTCGATGTGGGATAGTAAGAACTGGGATGGAGTTCTTGAGATGTTAACATCATGTCAAGCCAATCCCATCTCAACATCTCAGATGCATAAATGGATGGGAAGTTGGTTAATGTCTGATAATCATGATGCCAGTCAAGGGTATAGTTTTTTACATGAAGTGGACAAAGAGGCAGAAATAACATTTGACGTGGTGGAGACCTTCATCCGCGGCTGGGGCAACAAACCAATTGAATACATCAAAAAGGAAAGATGGACTGACTCATTCAAAATTCTCGCTTATTTGTGTCAAAAGTTTTTGGACTTACACAAGTTGACATTAATCTTAAATGCTGTCTCTGAGGTGGAATTGCTCAACTTGGCGAGGACTTTCAAAGGCAAAGTCAGAAGAAGTTCTCATGGAACGAACATATGCAGGATTAGGGTTCCCAGCTTGGGTCCTACTTTTATTTCAGAAGGATGGGCTTACTTCAAGAAACTTGATATTCTAATGGACCGAAACTTTCTGTTAATGGTCAAAGATGTGATTATAGGGAGGATGCAAACGGTGCTATCCATGGTATGTAGAATAGACAACCTGTTCTCAGAGCAAGACATCTTCTCCCTTCTAAATATCTACAGAATTGGAGATAAAATTGTGGAGAGGCAGGGAAATTTTTCTTATGACTTGATTAAAATGGTGGAACCGATATGCAACTTGAAGCTGATGAAATTAGCAAGAGAATCAAGGCCTTTAGTCCCACAATTCCCTCATTTTGAAAATCATATCAAGACTTCTGTTGATGAAGGGGCAAAAATTGACCGAGGTATAAGATTCCTCCATGATCAGATAATGAGTGTGAAAACAGTGGATCTCACACTGGTGATTTATGGATCGTTCAGACATTGGGGTCATCCTTTTATAGATTATTACACTGGACTAGAAAAATTACATTCCCAAGTAACCATGAAGAAAGATATTGATGTGTCATATGCAAAAGCACTTGCAAGTGATTTAGCTCGGATTGTTCTATTTCAACAGTTCAATGATCATAAAAAGTGGTTCGTGAATGGAGACTTGCTCCCTCATGATCATCCCTTTAAAAGTCATGTTAAAGAAAATACATGGCCCACAGCTGCTCAAGTTCAAGATTTTGGAGATAAATGGCATGAACTTCCGCTGATTAAATGTTTTGAAATACCCGACTTACTAGACCCATCGATAATATACTCTGACAAAAGTCATTCAATGAATAGGTCAGAGGTGTTGAAACATGTCCGAATGAATCCGAACACTCCTATCCCTAGTAAAAAGGTGTTGCAGACTATGTTGGACACAAAGGCTACCAATTGGAAAGAATTTCTTAAAGAGATTGATGAGAAGGGCTTAGATGATGATGATCTAATTATTGGTCTTAAAGGAAAGGAGAGGGAACTGAAGTTGGCAGGTAGATTTTTCTCCCTAATGTCTTGGAAATTGCGAGAATACTTTGTAATTACCGAATATTTGATAAAGACTCATTTCGTCCCTATGTTTAAAGGCCTGACAATGGCGGACGATCTAACTGCAGTCATTAAAAAGATGTTAGATTCCTCATCCGGCCAAGGATTGAAGTCATATGAGGCAATTTGCATAGCCAATCACATTGATTACGAAAAATGGAATAACCACCAAAGGAAGTTATCAAACGGCCCAGTGTTCCGAGTTATGGGCCAGTTCTTAGGTTATCCATCCTTAATCGAGAGAACTCATGAATTTTTTGAGAAAAGTCTTATATACTACAATGGAAGACCAGACTTGATGCGTGTTCACAACAACACACTGATCAATTCAACCTCCCAACGAGTTTGTTGGCAAGGACAAGAGGGTGGACTGGAAGGTCTACGGCAAAAAGGATGGACTATCCTCAATCTACTGGTTATTCAAAGAGAGGCTAAAATCAGAAACACTGCTGTCAAAGTCTTGGCACAAGGTGATAATCAAGTTATTTGCACACAGTATAAAACGAAGAAATCGAGAAACGTTGTAGAATTACAGGGTGCTCTCAATCAAATGGTTTCTAATAATGAGAAAATTATGACTGCAATCAAAATAGGGACAGGGAAGTTAGGACTTTTGATAAATGACGATGAGACTATGCAATCTGCAGATTACTTGAATTATGGAAAAATACCGATTTTCCGTGGAGTGATTAGAGGGTTAGAGACCAAGAGATGGTCACGAGTGACTTGTGTCACCAATGACCAAATACCCACTTGTGCTAATATAATGAGCTCAGTTTCCACAAATGCTCTCACCGTAGCTCATTTTGCTGAGAACCCAATCAATGCCATGATACAGTACAATTATTTTGGGACATTTGCTAGACTCTTGTTGATGATGCATGATCCTGCTCTTCGTCAATCATTGTATGAAGTTCAAGATAAGATACCGGGCTTGCACAGTTCTACTTTCAAATACGCCATGTTGTATTTGGACCCTTCCATTGGAGGAGTGTCGGGCATGTCTTTGTCCAGGTTTTTGATTAGAGCCTTCCCAGATCCCGTAACAGAAAGTCTCTCATTCTGGAGATTCATCCATGTACATGCTCGAAGTGAGCATCTGAAGGAGATGAGTGCAGTATTTGGAAACCCCGAGATAGCCAAGTTTCGAATAACTCACATAGACAAGCTAGTAGAAGATCCAACCTCTCTGAACATCGCTATGGGAATGAGTCCAGCGAACTTGTTAAAGACTGAGGTTAAAAAATGCTTAATCGAATCAAGACAAACCATCAGGAACCAGGTGATTAAGGATGCAACCATATATTTGTATCATGAAGAGGATCGGCTCAGAAGTTTCTTATGGTCAATAAATCCTCTGTTCCCTAGATTTTTAAGTGAATTCAAATCAGGCACTTTTTTGGGAGTCGCAGACGGGCTCATCAGTCTATTTCAAAATTCTCGTACTATTCGGAACTCCTTTAAGAAAAAGTATCATAGGGAATTGGATGATTTGATTGTGAGGAGTGAGGTATCCTCTTTGACACATTTAGGGAAACTTCATTTGAGAAGGGGATCATGTAAAATGTGGACATGTTCAGCTACTCATGCTGACACATTAAGATACAAATCCTGGGGCCGTACAGTTATTGGGACAACTGTACCCCATCCATTAGAAATGTTGGGTCCACAACATCGAAAAGAGACTCCTTGTGCACCATGTAACACATCAGGGTTCAATTATGTTTCTGTGCATTGTCCAGACGGGATCCATGACGTCTTTAGTTCACGGGGACCATTGCCTGCTTATCTAGGGTCTAAAACATCTGAATCTACATCTATTTTGCAGCCTTGGGAAAGGGAAAGCAAAGTCCCACTGATTAAAAGAGCTACACGTCTTAGAGATGCTATCTCTTGGTTTGTTGAACCCGACTCTAAACTAGCAATGACTATACTTTCTAACATCCACTCTTTAACAGGCGAAGAATGGACCAAAAGGCAGCATGGGTTCAAAAGAACAGGGTCTGCCCTTCATAGGTTTTCGACATCTCGGATGAGCCATGGTGGGTTCGCATCTCAGAGCACTGCAGCATTGACCAGGTTGATGGCAACTACAGACACCATGAGGGATCTGGGAGATCAGAATTTCGACTTTTTATTCCAAGCAACGTTGCTCTATGCTCAAATTACCACCACTGTTGCAAGAGACGGATGGATCACCAGTTGTACAGATCATTATCATATTGCCTGTAAGTCCTGTTTGAGACCCATAGAAGAGATCACCCTGGACTCAAGTATGGACTACACGCCCCCAGATGTATCCCATGTGCTGAAGACATGGAGGAATGGGGAAGGTTCGTGGGGACAAGAGATAAAACAGATCTATCCTTTAGAAGGGAATTGGAAGAATTTAGCACCTGCTGAGCAATCCTATCAAGTCGGCAGATGTATAGGTTTTCTATATGGAGACTTGGCGTATAGAAAATCTACTCATGCCGAGGACAGTTCTCTATTTCCTCTATCTATACAAGGTCGTATTAGAGGTCGAGGTTTCTTAAAAGGGTTGCTAGACGGATTAATGAGAGCAAGTTGCTGCCAAGTAATACACCGGAGAAGTCTGGCTCATTTGAAGAGGCCGGCCAACGCAGTGTACGGAGGTTTGATTTACTTGATTGATAAATTGAGTGTATCACCTCCATTCCTTTCTCTTACTAGATCAGGACCTATTAGAGACGAATTAGAAACGATTCCCCACAAGATCCCAACCTCCTATCCGACAAGCAACCGTGATATGGGGGTGATTGTCAGAAATTACTTCAAATACCAATGCCGTCTAATTGAAAAGGGAAAATACAGATCACATTATTCACAATTATGGTTATTCTCAGATGTCTTATCCATAGACTTCATTGGACCATTCTCTATTTCCACCACCCTCTTGCAAATCCTATACAAGCCATTTTTATCTGGGAAAGATAAGAATGAGTTGAGAGAGCTGGCAAATCTTTCTTCATTGCTAAGATCAGGAGAGGGGTGGGAAGACATACATGTGAAATTCTTCACCAAGGACATATTATTGTGTCCAGAGGAAATCAGACATGCTTGCAAGTTCGGGATTGCTAAGGATAATAATAAAGACATGAGCTATCCCCCTTGGGGAAGGGAATCCAGAGGGACAATTACAACAATCCCTGTTTATTATACGACCACCCCTTACCCAAAGATGCTAGAGATGCCTCCAAGAATCCAAAATCCCCTGCTGTCCGGAATCAGGTTGGGCCAATTACCAACTGGCGCTCATTATAAAATTCGGAGTATATTACATGGAATGGGAATCCATTACAGGGACTTCTTGAGTTGTGGAGACGGCTCCGGAGGGATGACTGCTGCATTACTACGAGAAAATGTGCATAGCAGAGGAATATTCAATAGTCTGTTAGAATTATCAGGGTCAGTCATGCGAGGCGCCTCTCCTGAGCCCCCCAGTGCCCTAGAAACTTTAGGAGGAGATAAATCGAGATGTGTAAATGGTGAAACATGTTGGGAATATCCATCTGACTTATGTGACCCAAGGACTTGGGACTATTTCCTCCGACTCAAAGCAGGCTTGGGGCTTCAAATTGATTTAATTGTAATGGATATGGAAGTTCGGGATTCTTCTACTAGCCTGAAAATTGAGACGAATGTTAGAAATTATGTGCACCGGATTTTGGATGAGCAAGGAGTTTTAATCTACAAGACTTATGGAACATATATTTGTGAGAGCGAAAAGAATGCAGTAACAATCCTTGGTCCCATGTTCAAGACGGTCGACTTAGTTCAAACAGAATTTAGTAGTTCTCAAACGTCTGAAGTATATATGGTATGTAAAGGTTTGAAGAAATTAATCGATGAACCCAATCCCGATTGGTCTTCCATCAATGAATCCTGGAAAAACCTGTACGCATTCCAGTCATCAGAACAGGAATTTGCCAGAGCAAAGAAGGTTAGTACATACTTTACCTTGACAGGTATTCCCTCCCAATTCATTCCTGATCCTTTTGTAAACATTGAGACTATGCTACAAATATTCGGAGTACCCACGGGTGTGTCTCATGCGGCTGCCTTAAAATCATCTGATAGACCTGCAGATTTATTGACCATTAGCCTTTTTTATATGGCGATTATATCGTATTATAACATCAATCATATCAGAGTAGGACCGATACCTCCGAACCCCCCATCAGATGGAATTGCACAAAATGTGGGGATCGCTATAACTGGTATAAGCTTTTGGCTGAGTTTGATGGAGAAAGACATTCCACTATATCAACAGTGTTTAGCAGTTATCCAGCAATCATTCCCGATTAGGTGGGAGGCTGTTTCAGTAAAAGGAGGATACAAGCAGAAGTGGAGTACTAGAGGTGATGGGCTCCCAAAAGATACCCGAACTTCAGACTCCTTGGCCCCAATCGGGAACTGGATCAGATCTCTGGAATTGGTCCGAAACCAAGTTCGTCTAAATCCATTCAATGAGATCTTGTTCAATCAGCTATGTCGTACAGTGGATAATCATTTGAAATGGTCAAATTTGCGAAGAAACACAGGAATGATTGAATGGATCAATAGACGAATTTCAAAAGAAGACCGGTCTATACTGATGTTGAAGAGTGACCTACACGAGGAAAACTCTTGGAGAGATTAAAAAATCATGAGGAGACTCCAAACTTTAAGTATGAAAAAAACTTTGATCCTTAAGACCCTCTTGTGGTTTTTATTTTTTATCTGGTTTTGTGGTCTTCGT。在另一个实施方式中，核酸包括SEQ ID NO：23的序列，如下所示(VSV载体：Convac V3 South Africa)：
ACGAAGACAAACAAACCATTATTATCATTAAAAGGCTCAGGAGAAACTTTAACAGTAATCAAAATGTCTGTTACAGTCAAGAGAATCATTGACAACACAGTCATAGTTCCAAAACTTCCTGCAAATGAGGATCCAGTGGAATACCCGGCAGATTACTTCAGAAAATCAAAGGAGATTCCTCTTTACATCAATACTACAAAAAGTTTGTCAGATCTAAGAGGATATGTCTACCAAGGCCTCAAATCCGGAAATGTATCAATCATACATGTCAACAGCTACTTGTATGGAGCATTAAAGGACATCCGGGGTAAGTTGGATAAAGATTGGTCAAGTTTCGGAATAAACATCGGGAAAGCAGGGGATACAATCGGAATATTTGACCTTGTATCCTTGAAAGCCCTGGACGGCGTACTTCCAGATGGAGTATCGGATGCTTCCAGAACCAGCGCAGATGACAAATGGTTGCCTTTGTATCTACTTGGCTTATACAGAGTGGGCAGAACACAAATGCCTGAATACAGAAAAAAGCTCATGGATGGGCTGACAAATCAATGCAAAATGATCAATGAACAGTTTGAACCTCTTGTGCCAGAAGGTCGTGACATTTTTGATGTGTGGGGAAATGACAGTAATTACACAAAAATTGTCGCTGCAGTGGACATGTTCTTCCACATGTTCAAAAAACATGAATGTGCCTCGTTCAGATACGGAACTATTGTTTCCAGATTCAAAGATTGTGCTGCATTGGCAACATTTGGACACCTCTGCAAAATAACCGGAATGTCTACAGAAGATGTAACGACCTGGATCTTGAACCGAGAAGTTGCAGATGAAATGGTCCAAATGATGCTTCCAGGCCAAGAAATTGACAAGGCCGATTCATACATGCCTTATTTGATCGACTTTGGATTGTCTTCTAAGTCTCCATATTCTTCCGTCAAAAACCCTGCCTTCCACTTCTGGGGGCAATTGACAGCTCTTCTGCTCAGATCCACCAGAGCAAGGAATGCCCGACAGCCTGATGACATTGAGTATACATCTCTTACTACAGCAGGTTTGTTGTACGCTTATGCAGTAGGATCCTCTGCCGACTTGGCACAACAGTTTTGTGTTGGAGATAACAAATACACTCCAGATGATAGTACCGGAGGATTGACGACTAATGCACCGCCACAAGGCAGAGATGTGGTCGAATGGCTCGGATGGTTTGAAGATCAAAACAGAAAACCGACTCCTGATATGATGCAGTATGCGAAAAGAGCAGTCATGTCACTGCAAGGCCTAAGAGAGAAGACAATTGGCAAGTATGCTAAGTCAGAATTTGACAAATGACCCTATAATTCTCAGATCACCTATTATATATTATGCTACATATGAAAAAAACTAACAGATATCATGGATAATCTCACAAAAGTTCGTGAGTATCTCAAGTCCTATTCTCGTCTGGATCAGGCGGTAGGAGAGATAGATGAGATCGAAGCACAACGAGCTGAAAAGTCCAATTATGAGTTGTTCCAAGAGGATGGAGTGGAAGAGCATACTAAGCCCTCTTATTTTCAGGCAGCAGATGATTCTGACACAGAATCTGAACCAGAAATTGAAGACAATCAAGGTTTGTATGCACCAGATCCAGAAGCTGAGCAAGTTGAAGGCTTTATACAGGGGCCTTTAGATGACTATGCAGATGAGGAAGTGGATGTTGTATTTACTTCGGACTGGAAACAGCCTGAGCTTGAATCTGACGAGCATGGAAAGACCTTACGGTTGACATCGCCAGAGGGTTTAAGTGGAGAGCAGAAATCCCAGTGGCTTTCGACGATTAAAGCAGTCGTGCAAAGTGCCAAATACTGGAATCTGGCAGAGTGCACATTTGAAGCATCGGGAGAAGGGGTCATTATGAAGGAGCGCCAGATAACTCCGGATGTATATAAGGTCACTCCAGTGATGAACACACATCCGTCCCAATCAGAAGCAGTATCAGATGTTTGGTCTCTCTCAAAGACATCCATGACTTTCCAACCCAAGAAAGCAAGTCTTCAGCCTCTCACCATATCCTTGGATGAATTGTTCTCATCTAGAGGAGAGTTCATCTCTGTCGGAGGTGACGGACGAATGTCTCATAAAGAGGCCATCCTGCTCGGCCTGAGATACAAAAAGTTGTACAATCAGGCGAGAGTCAAATATTCTCTGTAGACTATGAAAAAAAGTAACAGATATCACGATCTAAGTGTTATCCCAATCCATTCATCATGAGTTCCTTAAAGAAGATTCTCGGTCTGAAGGGGAAAGGTAAGAAATCTAAGAAATTAGGGATCGCACCACCCCCTTATGAAGAGGACACTAGCATGGAGTATGCTCCGAGCGCTCCAATTGACAAATCCTATTTTGGAGTTGACGAGATGGACACCTATGATCCGAATCAATTAAGATATGAGAAATTCTTCTTTACAGTGAAAATGACGGTTAGATCTAATCGTCCGTTCAGAACATACTCAGATGTGGCAGCCGCTGTATCCCATTGGGATCACATGTACATCGGAATGGCAGGGAAACGTCCCTTCTACAAAATCTTGGCTTTTTTGGGTTCTTCTAATCTAAAGGCCACTCCAGCGGTATTGGCAGATCAAGGTCAACCAGAGTATCACACTCACTGCGAAGGCAGGGCTTATTTGCCACATAGGATGGGGAAGACCCCTCCCATGCTCAATGTACCAGAGCACTTCAGAAGACCATTCAATATAGGTCTTTACAAGGGAACGATTGAGCTCACAATGACCATCTACGATGATGAGTCACTGGAAGCAGCTCCTATGATCTGGGATCATTTCAATTCTTCCAAATTTTCTGATTTCAGAGAGAAGGCCTTAATGTTTGGCCTGATTGTCGAGAAAAAGGCATCTGGAGCGTGGGTCCTGGATTCTATCAGCCACTTCAAATGAGCTAGTCTAACTTCTAGCTTCTGAACAATCCCCGGTTTACTCAGTCTCTCCTAATTCCAGCCTCTCGAACAACTAATATCCTGTCTTTTCTATCCCTATGAAAAAAACTAACAGAGATCGATCTGTTTACGCGTCACTATGAAGTGCCTTTTGTACTTAGCCTTTTTATTCATTGGGGTGAATTGCAAGTTCACCATAGTTTTTCCACACAACCAAAAAGGAAACTGGAAAAATGTTCCTTCTAATTACCATTATTGCCCGTCAAGCTCAGATTTAAATTGGCATAATGACTTAATAGGCACAGCCATACAAGTCAAAATGCCCAAGAGTCACAAGGCTATTCAAGCAGACGGTTGGATGTGTCATGCTTCCAAATGGGTCACTACTTGTGATTTCCGCTGGTATGGACCGAAGTATATAACACAGTCCATCCGATCCTTCACTCCATCTGTAGAACAATGCAAGGAAAGCATTGAACAAACGAAACAAGGAACTTGGCTGAATCCAGGCTTCCCTCCTCAAAGTTGTGGATATGCAACTGTGACGGATGCCGAAGCAGTGATTGTCCAGGTGACTCCTCACCATGTGCTGGTTGATGAATACACAGGAGAATGGGTTGATTCACAGTTCATCAACGGAAAATGCAGCAATTACATATGCCCCACTGTCCATAACTCTACAACCTGGCATTCTGACTATAAGGTCAAAGGGCTATGTGATTCTAACCTCATTTCCATGGACATCACCTTCTTCTCAGAGGACGGAGAGCTATCATCCCTGGGAAAGGAGGGCACAGGGTTCAGAAGTAACTACTTTGCTTATGAAACTGGAGGCAAGGCCTGCAAAATGCAATACTGCAAGCATTGGGGAGTCAGACTCCCATCAGGTGTCTGGTTCGAGATGGCTGATAAGGATCTCTTTGCTGCAGCCAGATTCCCTGAATGCCCAGAAGGGTCAAGTATCTCTGCTCCATCTCAGACCTCAGTGGATGTAAGTCTAATTCAGGACGTTGAGAGGATCTTGGATTATTCCCTCTGCCAAGAAACCTGGAGCAAAATCAGAGCGGGTCTTCCAATCTCTCCAGTGGATCTCAGCTATCTTGCTCCTAAAAACCCAGGAACCGGTCCTGCTTTCACCATAATCAATGGTACCCTAAAATACTTTGAGACCAGATACATCAGAGTCGATATTGCTGCTCCAATCCTCTCAAGAATGGTCGGAATGATCAGTGGAACTACCACAGAAAGGGAACTGTGGGATGACTGGGCACCATATGAAGACGTGGAAATTGGACCCAATGGAGTTCTGAGGACCAGTTCAGGATATAAGTTTCCTTTATACATGATTGGACATGGTATGTTGGACTCCGATCTTCATCTTAGCTCAAAGGCTCAGGTGTTCGAACATCCTCACATTCAAGACGCTGCTTCGCAACTTCCTGATGATGAGAGTTTATTTTTTGGTGATACTGGGCTATCCAAAAATCCAATCGAGCTTGTAGAAGGTTGGTTCAGTAGTTGGAAAAGCTCTATTGCCTCTTTTTTCTTTATCATAGGGTTAATCATTGGACTATTCTTGGTTCTCCGAGTTGGTATCCATCTTTGCATTAAATTAAAGCACACCAAGAAAAGACAGATTTATACAGACATAGAGATGAACCGACTTGGAAAGTAACTCAAATCCTGCTAGGTATGAAAAAAACTAACaGATATCACGCtcgagGCCACCATGTTCGTGTTTCTGGTGCTGCTGCCTCTGGTGAGCTCCCAGTGCGTGAACTTCACCACAAGGACCCAGCTGCCCCCTGCCTATACCAATTCCTTCACACGGGGCGTGTACTATCCCGACAAGGTGTTCCGGAGCAGCGTGCTGCACTCCACACAGGATCTGTTTCTGCCTTTCTTTTCTAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAATGGCACAAAGCGGTTCGCCAATCCAGTGCTGCCCTTTAACGATGGCGTGTACTTCGCCTCCACCGAGAAGTCTAACATCATCAGAGGCTGGATCTTTGGCACCACACTGGACAGCAAGACACAGTCCCTGCTGATCGTGAACAATGCCACCAACGTGGTCATCAAGGTGTGCGAGTTCCAGTTTTGTAATGATCCATTCCTGGGCGTGTACTATCACAAGAACAATAAGTCTTGGATGGAGAGCGAGTTTCGCGTGTATTCCTCTGCCAACAATTGCACATTTGAGTACGTGTCCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAATTTCAAGAACCTGAGGGAGTTCGTGTTTAAGAATATCGATGGCTACTTCAAAATCTACTCCAAGCACACCCCAATCAACCTGGTGCGCGGCCTGCCACAGGGCTTCTCTGCCCTGGAGCCACTGGTGGATCTGCCCATCGGCATCAACATCACCCGGTTTCAGACACTGCTGGCCCTGCACAGAAGCTACCTGACACCAGGCGACAGCTCCTCTGGATGGACCGCAGGAGCAGCAGCCTACTATGTGGGCTATCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAATGGCACCATCACAGACGCCGTGGATTGCGCCCTGGATCCCCTGTCTGAGACCAAGTGTACACTGAAGAGCTTTACCGTGGAGAAGGGCATCTATCAGACAAGCAATTTCAGGGTGCAGCCTACCGAGTCCATCGTGCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAATATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGAAGGGCTTTAACTGTTATTTCCCTCTGCAGAGCTACGGCTTCCAGCCAACATATGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACCTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGACTGACCGGAACAGGCGTGCTGACCGAGTCCAACAAGAAGTTCCTGCCTTTTCAGCAGTTCGGCAGGGACATCGCAGATACCACAGACGCCGTGCGCGACCCTCAGACCCTGGAGATCCTGGACATCACACCATGCTCCTTCGGCGGCGTGTCTGTGATCACACCAGGCACCAATACAAGCAACCAGGTGGCCGTGCTGTATCAGGGCGTGAATTGTACCGAGGTGCCAGTGGCAATCCACGCAGATCAGCTGACCCCTACATGGCGGGTGTACTCTACCGGCAGCAACGTGTTCCAGACAAGAGCCGGATGCCTGATCGGAGCAGAGCACGTGAACAATAGCTATGAGTGCGACATCCCTATCGGCGCCGGCATCTGTGCCTCCTACCAGACCCAGACAAACTCCCCAAGGGGATCCGGCTACATCCCCGAGGCCCCCAGAGACGGCCAGGCCTACGTGCGGAAGGACGGCGAGTGGGTACTGctcagcaccttcctgggcAGCAGTTGGAAAAGCTCCATCGCCTCCTTTTTCTTTATCATCGGCCTGATCATCGGACTGTTCCTGGTGCTCCGCGTGGGTATCCACCTGTGCATCAAGCTGAAGCACACCAAGAAAAGACAGATTTATACAGACATCGAGATGAACCGACTTGGAAAGTAAGCTAGCCAGATTCTTCATGTTTGGACCAAATCAACTTGTGATACCATGCTCAAAGAGGCCTCAATTATATTTGAGTTTTTAATTTTTATGAAAAAAACTAACAGCAATCATGGAAGTCCACGATTTTGAGACCGACGAGTTCAATGATTTCAATGAAGATGACTATGCCACAAGAGAATTCCTGAATCCCGATGAGCGCATGACGTACTTGAATCATGCTGATTACAATTTGAATTCTCCTCTAATTAGTGATGATATTGACAATTTGATCAGGAAATTCAATTCTCTTCCGATTCCCTCGATGTGGGATAGTAAGAACTGGGATGGAGTTCTTGAGATGTTAACATCATGTCAAGCCAATCCCATCTCAACATCTCAGATGCATAAATGGATGGGAAGTTGGTTAATGTCTGATAATCATGATGCCAGTCAAGGGTATAGTTTTTTACATGAAGTGGACAAAGAGGCAGAAATAACATTTGACGTGGTGGAGACCTTCATCCGCGGCTGGGGCAACAAACCAATTGAATACATCAAAAAGGAAAGATGGACTGACTCATTCAAAATTCTCGCTTATTTGTGTCAAAAGTTTTTGGACTTACACAAGTTGACATTAATCTTAAATGCTGTCTCTGAGGTGGAATTGCTCAACTTGGCGAGGACTTTCAAAGGCAAAGTCAGAAGAAGTTCTCATGGAACGAACATATGCAGGATTAGGGTTCCCAGCTTGGGTCCTACTTTTATTTCAGAAGGATGGGCTTACTTCAAGAAACTTGATATTCTAATGGACCGAAACTTTCTGTTAATGGTCAAAGATGTGATTATAGGGAGGATGCAAACGGTGCTATCCATGGTATGTAGAATAGACAACCTGTTCTCAGAGCAAGACATCTTCTCCCTTCTAAATATCTACAGAATTGGAGATAAAATTGTGGAGAGGCAGGGAAATTTTTCTTATGACTTGATTAAAATGGTGGAACCGATATGCAACTTGAAGCTGATGAAATTAGCAAGAGAATCAAGGCCTTTAGTCCCACAATTCCCTCATTTTGAAAATCATATCAAGACTTCTGTTGATGAAGGGGCAAAAATTGACCGAGGTATAAGATTCCTCCATGATCAGATAATGAGTGTGAAAACAGTGGATCTCACACTGGTGATTTATGGATCGTTCAGACATTGGGGTCATCCTTTTATAGATTATTACACTGGACTAGAAAAATTACATTCCCAAGTAACCATGAAGAAAGATATTGATGTGTCATATGCAAAAGCACTTGCAAGTGATTTAGCTCGGATTGTTCTATTTCAACAGTTCAATGATCATAAAAAGTGGTTCGTGAATGGAGACTTGCTCCCTCATGATCATCCCTTTAAAAGTCATGTTAAAGAAAATACATGGCCCACAGCTGCTCAAGTTCAAGATTTTGGAGATAAATGGCATGAACTTCCGCTGATTAAATGTTTTGAAATACCCGACTTACTAGACCCATCGATAATATACTCTGACAAAAGTCATTCAATGAATAGGTCAGAGGTGTTGAAACATGTCCGAATGAATCCGAACACTCCTATCCCTAGTAAAAAGGTGTTGCAGACTATGTTGGACACAAAGGCTACCAATTGGAAAGAATTTCTTAAAGAGATTGATGAGAAGGGCTTAGATGATGATGATCTAATTATTGGTCTTAAAGGAAAGGAGAGGGAACTGAAGTTGGCAGGTAGATTTTTCTCCCTAATGTCTTGGAAATTGCGAGAATACTTTGTAATTACCGAATATTTGATAAAGACTCATTTCGTCCCTATGTTTAAAGGCCTGACAATGGCGGACGATCTAACTGCAGTCATTAAAAAGATGTTAGATTCCTCATCCGGCCAAGGATTGAAGTCATATGAGGCAATTTGCATAGCCAATCACATTGATTACGAAAAATGGAATAACCACCAAAGGAAGTTATCAAACGGCCCAGTGTTCCGAGTTATGGGCCAGTTCTTAGGTTATCCATCCTTAATCGAGAGAACTCATGAATTTTTTGAGAAAAGTCTTATATACTACAATGGAAGACCAGACTTGATGCGTGTTCACAACAACACACTGATCAATTCAACCTCCCAACGAGTTTGTTGGCAAGGACAAGAGGGTGGACTGGAAGGTCTACGGCAAAAAGGATGGACTATCCTCAATCTACTGGTTATTCAAAGAGAGGCTAAAATCAGAAACACTGCTGTCAAAGTCTTGGCACAAGGTGATAATCAAGTTATTTGCACACAGTATAAAACGAAGAAATCGAGAAACGTTGTAGAATTACAGGGTGCTCTCAATCAAATGGTTTCTAATAATGAGAAAATTATGACTGCAATCAAAATAGGGACAGGGAAGTTAGGACTTTTGATAAATGACGATGAGACTATGCAATCTGCAGATTACTTGAATTATGGAAAAATACCGATTTTCCGTGGAGTGATTAGAGGGTTAGAGACCAAGAGATGGTCACGAGTGACTTGTGTCACCAATGACCAAATACCCACTTGTGCTAATATAATGAGCTCAGTTTCCACAAATGCTCTCACCGTAGCTCATTTTGCTGAGAACCCAATCAATGCCATGATACAGTACAATTATTTTGGGACATTTGCTAGACTCTTGTTGATGATGCATGATCCTGCTCTTCGTCAATCATTGTATGAAGTTCAAGATAAGATACCGGGCTTGCACAGTTCTACTTTCAAATACGCCATGTTGTATTTGGACCCTTCCATTGGAGGAGTGTCGGGCATGTCTTTGTCCAGGTTTTTGATTAGAGCCTTCCCAGATCCCGTAACAGAAAGTCTCTCATTCTGGAGATTCATCCATGTACATGCTCGAAGTGAGCATCTGAAGGAGATGAGTGCAGTATTTGGAAACCCCGAGATAGCCAAGTTTCGAATAACTCACATAGACAAGCTAGTAGAAGATCCAACCTCTCTGAACATCGCTATGGGAATGAGTCCAGCGAACTTGTTAAAGACTGAGGTTAAAAAATGCTTAATCGAATCAAGACAAACCATCAGGAACCAGGTGATTAAGGATGCAACCATATATTTGTATCATGAAGAGGATCGGCTCAGAAGTTTCTTATGGTCAATAAATCCTCTGTTCCCTAGATTTTTAAGTGAATTCAAATCAGGCACTTTTTTGGGAGTCGCAGACGGGCTCATCAGTCTATTTCAAAATTCTCGTACTATTCGGAACTCCTTTAAGAAAAAGTATCATAGGGAATTGGATGATTTGATTGTGAGGAGTGAGGTATCCTCTTTGACACATTTAGGGAAACTTCATTTGAGAAGGGGATCATGTAAAATGTGGACATGTTCAGCTACTCATGCTGACACATTAAGATACAAATCCTGGGGCCGTACAGTTATTGGGACAACTGTACCCCATCCATTAGAAATGTTGGGTCCACAACATCGAAAAGAGACTCCTTGTGCACCATGTAACACATCAGGGTTCAATTATGTTTCTGTGCATTGTCCAGACGGGATCCATGACGTCTTTAGTTCACGGGGACCATTGCCTGCTTATCTAGGGTCTAAAACATCTGAATCTACATCTATTTTGCAGCCTTGGGAAAGGGAAAGCAAAGTCCCACTGATTAAAAGAGCTACACGTCTTAGAGATGCTATCTCTTGGTTTGTTGAACCCGACTCTAAACTAGCAATGACTATACTTTCTAACATCCACTCTTTAACAGGCGAAGAATGGACCAAAAGGCAGCATGGGTTCAAAAGAACAGGGTCTGCCCTTCATAGGTTTTCGACATCTCGGATGAGCCATGGTGGGTTCGCATCTCAGAGCACTGCAGCATTGACCAGGTTGATGGCAACTACAGACACCATGAGGGATCTGGGAGATCAGAATTTCGACTTTTTATTCCAAGCAACGTTGCTCTATGCTCAAATTACCACCACTGTTGCAAGAGACGGATGGATCACCAGTTGTACAGATCATTATCATATTGCCTGTAAGTCCTGTTTGAGACCCATAGAAGAGATCACCCTGGACTCAAGTATGGACTACACGCCCCCAGATGTATCCCATGTGCTGAAGACATGGAGGAATGGGGAAGGTTCGTGGGGACAAGAGATAAAACAGATCTATCCTTTAGAAGGGAATTGGAAGAATTTAGCACCTGCTGAGCAATCCTATCAAGTCGGCAGATGTATAGGTTTTCTATATGGAGACTTGGCGTATAGAAAATCTACTCATGCCGAGGACAGTTCTCTATTTCCTCTATCTATACAAGGTCGTATTAGAGGTCGAGGTTTCTTAAAAGGGTTGCTAGACGGATTAATGAGAGCAAGTTGCTGCCAAGTAATACACCGGAGAAGTCTGGCTCATTTGAAGAGGCCGGCCAACGCAGTGTACGGAGGTTTGATTTACTTGATTGATAAATTGAGTGTATCACCTCCATTCCTTTCTCTTACTAGATCAGGACCTATTAGAGACGAATTAGAAACGATTCCCCACAAGATCCCAACCTCCTATCCGACAAGCAACCGTGATATGGGGGTGATTGTCAGAAATTACTTCAAATACCAATGCCGTCTAATTGAAAAGGGAAAATACAGATCACATTATTCACAATTATGGTTATTCTCAGATGTCTTATCCATAGACTTCATTGGACCATTCTCTATTTCCACCACCCTCTTGCAAATCCTATACAAGCCATTTTTATCTGGGAAAGATAAGAATGAGTTGAGAGAGCTGGCAAATCTTTCTTCATTGCTAAGATCAGGAGAGGGGTGGGAAGACATACATGTGAAATTCTTCACCAAGGACATATTATTGTGTCCAGAGGAAATCAGACATGCTTGCAAGTTCGGGATTGCTAAGGATAATAATAAAGACATGAGCTATCCCCCTTGGGGAAGGGAATCCAGAGGGACAATTACAACAATCCCTGTTTATTATACGACCACCCCTTACCCAAAGATGCTAGAGATGCCTCCAAGAATCCAAAATCCCCTGCTGTCCGGAATCAGGTTGGGCCAATTACCAACTGGCGCTCATTATAAAATTCGGAGTATATTACATGGAATGGGAATCCATTACAGGGACTTCTTGAGTTGTGGAGACGGCTCCGGAGGGATGACTGCTGCATTACTACGAGAAAATGTGCATAGCAGAGGAATATTCAATAGTCTGTTAGAATTATCAGGGTCAGTCATGCGAGGCGCCTCTCCTGAGCCCCCCAGTGCCCTAGAAACTTTAGGAGGAGATAAATCGAGATGTGTAAATGGTGAAACATGTTGGGAATATCCATCTGACTTATGTGACCCAAGGACTTGGGACTATTTCCTCCGACTCAAAGCAGGCTTGGGGCTTCAAATTGATTTAATTGTAATGGATATGGAAGTTCGGGATTCTTCTACTAGCCTGAAAATTGAGACGAATGTTAGAAATTATGTGCACCGGATTTTGGATGAGCAAGGAGTTTTAATCTACAAGACTTATGGAACATATATTTGTGAGAGCGAAAAGAATGCAGTAACAATCCTTGGTCCCATGTTCAAGACGGTCGACTTAGTTCAAACAGAATTTAGTAGTTCTCAAACGTCTGAAGTATATATGGTATGTAAAGGTTTGAAGAAATTAATCGATGAACCCAATCCCGATTGGTCTTCCATCAATGAATCCTGGAAAAACCTGTACGCATTCCAGTCATCAGAACAGGAATTTGCCAGAGCAAAGAAGGTTAGTACATACTTTACCTTGACAGGTATTCCCTCCCAATTCATTCCTGATCCTTTTGTAAACATTGAGACTATGCTACAAATATTCGGAGTACCCACGGGTGTGTCTCATGCGGCTGCCTTAAAATCATCTGATAGACCTGCAGATTTATTGACCATTAGCCTTTTTTATATGGCGATTATATCGTATTATAACATCAATCATATCAGAGTAGGACCGATACCTCCGAACCCCCCATCAGATGGAATTGCACAAAATGTGGGGATCGCTATAACTGGTATAAGCTTTTGGCTGAGTTTGATGGAGAAAGACATTCCACTATATCAACAGTGTTTAGCAGTTATCCAGCAATCATTCCCGATTAGGTGGGAGGCTGTTTCAGTAAAAGGAGGATACAAGCAGAAGTGGAGTACTAGAGGTGATGGGCTCCCAAAAGATACCCGAACTTCAGACTCCTTGGCCCCAATCGGGAACTGGATCAGATCTCTGGAATTGGTCCGAAACCAAGTTCGTCTAAATCCATTCAATGAGATCTTGTTCAATCAGCTATGTCGTACAGTGGATAATCATTTGAAATGGTCAAATTTGCGAAGAAACACAGGAATGATTGAATGGATCAATAGACGAATTTCAAAAGAAGACCGGTCTATACTGATGTTGAAGAGTGACCTACACGAGGAAAACTCTTGGAGAGATTAAAAAATCATGAGGAGACTCCAAACTTTAAGTATGAAAAAAACTTTGATCCTTAAGACCCTCTTGTGGTTTTTATTTTTTATCTGGTTTTGTGGTCTTCGT。
在一些实施方式中，核酸包括SEQ ID NO：24的序列，如下所示(VSV载体：ConvacV4 China)：
ACGAAGACAAACAAACCATTATTATCATTAAAAGGCTCAGGAGAAACTTTAACAGTAATCAAAATGTCTGTTACAGTCAAGAGAATCATTGACAACACAGTCATAGTTCCAAAACTTCCTGCAAATGAGGATCCAGTGGAATACCCGGCAGATTACTTCAGAAAATCAAAGGAGATTCCTCTTTACATCAATACTACAAAAAGTTTGTCAGATCTAAGAGGATATGTCTACCAAGGCCTCAAATCCGGAAATGTATCAATCATACATGTCAACAGCTACTTGTATGGAGCATTAAAGGACATCCGGGGTAAGTTGGATAAAGATTGGTCAAGTTTCGGAATAAACATCGGGAAAGCAGGGGATACAATCGGAATATTTGACCTTGTATCCTTGAAAGCCCTGGACGGCGTACTTCCAGATGGAGTATCGGATGCTTCCAGAACCAGCGCAGATGACAAATGGTTGCCTTTGTATCTACTTGGCTTATACAGAGTGGGCAGAACACAAATGCCTGAATACAGAAAAAAGCTCATGGATGGGCTGACAAATCAATGCAAAATGATCAATGAACAGTTTGAACCTCTTGTGCCAGAAGGTCGTGACATTTTTGATGTGTGGGGAAATGACAGTAATTACACAAAAATTGTCGCTGCAGTGGACATGTTCTTCCACATGTTCAAAAAACATGAATGTGCCTCGTTCAGATACGGAACTATTGTTTCCAGATTCAAAGATTGTGCTGCATTGGCAACATTTGGACACCTCTGCAAAATAACCGGAATGTCTACAGAAGATGTAACGACCTGGATCTTGAACCGAGAAGTTGCAGATGAAATGGTCCAAATGATGCTTCCAGGCCAAGAAATTGACAAGGCCGATTCATACATGCCTTATTTGATCGACTTTGGATTGTCTTCTAAGTCTCCATATTCTTCCGTCAAAAACCCTGCCTTCCACTTCTGGGGGCAATTGACAGCTCTTCTGCTCAGATCCACCAGAGCAAGGAATGCCCGACAGCCTGATGACATTGAGTATACATCTCTTACTACAGCAGGTTTGTTGTACGCTTATGCAGTAGGATCCTCTGCCGACTTGGCACAACAGTTTTGTGTTGGAGATAACAAATACACTCCAGATGATAGTACCGGAGGATTGACGACTAATGCACCGCCACAAGGCAGAGATGTGGTCGAATGGCTCGGATGGTTTGAAGATCAAAACAGAAAACCGACTCCTGATATGATGCAGTATGCGAAAAGAGCAGTCATGTCACTGCAAGGCCTAAGAGAGAAGACAATTGGCAAGTATGCTAAGTCAGAATTTGACAAATGACCCTATAATTCTCAGATCACCTATTATATATTATGCTACATATGAAAAAAACTAACAGATATCATGGATAATCTCACAAAAGTTCGTGAGTATCTCAAGTCCTATTCTCGTCTGGATCAGGCGGTAGGAGAGATAGATGAGATCGAAGCACAACGAGCTGAAAAGTCCAATTATGAGTTGTTCCAAGAGGATGGAGTGGAAGAGCATACTAAGCCCTCTTATTTTCAGGCAGCAGATGATTCTGACACAGAATCTGAACCAGAAATTGAAGACAATCAAGGTTTGTATGCACCAGATCCAGAAGCTGAGCAAGTTGAAGGCTTTATACAGGGGCCTTTAGATGACTATGCAGATGAGGAAGTGGATGTTGTATTTACTTCGGACTGGAAACAGCCTGAGCTTGAATCTGACGAGCATGGAAAGACCTTACGGTTGACATCGCCAGAGGGTTTAAGTGGAGAGCAGAAATCCCAGTGGCTTTCGACGATTAAAGCAGTCGTGCAAAGTGCCAAATACTGGAATCTGGCAGAGTGCACATTTGAAGCATCGGGAGAAGGGGTCATTATGAAGGAGCGCCAGATAACTCCGGATGTATATAAGGTCACTCCAGTGATGAACACACATCCGTCCCAATCAGAAGCAGTATCAGATGTTTGGTCTCTCTCAAAGACATCCATGACTTTCCAACCCAAGAAAGCAAGTCTTCAGCCTCTCACCATATCCTTGGATGAATTGTTCTCATCTAGAGGAGAGTTCATCTCTGTCGGAGGTGACGGACGAATGTCTCATAAAGAGGCCATCCTGCTCGGCCTGAGATACAAAAAGTTGTACAATCAGGCGAGAGTCAAATATTCTCTGTAGACTATGAAAAAAAGTAACAGATATCACGATCTAAGTGTTATCCCAATCCATTCATCATGAGTTCCTTAAAGAAGATTCTCGGTCTGAAGGGGAAAGGTAAGAAATCTAAGAAATTAGGGATCGCACCACCCCCTTATGAAGAGGACACTAGCATGGAGTATGCTCCGAGCGCTCCAATTGACAAATCCTATTTTGGAGTTGACGAGATGGACACCTATGATCCGAATCAATTAAGATATGAGAAATTCTTCTTTACAGTGAAAATGACGGTTAGATCTAATCGTCCGTTCAGAACATACTCAGATGTGGCAGCCGCTGTATCCCATTGGGATCACATGTACATCGGAATGGCAGGGAAACGTCCCTTCTACAAAATCTTGGCTTTTTTGGGTTCTTCTAATCTAAAGGCCACTCCAGCGGTATTGGCAGATCAAGGTCAACCAGAGTATCACACTCACTGCGAAGGCAGGGCTTATTTGCCACATAGGATGGGGAAGACCCCTCCCATGCTCAATGTACCAGAGCACTTCAGAAGACCATTCAATATAGGTCTTTACAAGGGAACGATTGAGCTCACAATGACCATCTACGATGATGAGTCACTGGAAGCAGCTCCTATGATCTGGGATCATTTCAATTCTTCCAAATTTTCTGATTTCAGAGAGAAGGCCTTAATGTTTGGCCTGATTGTCGAGAAAAAGGCATCTGGAGCGTGGGTCCTGGATTCTATCAGCCACTTCAAATGAGCTAGTCTAACTTCTAGCTTCTGAACAATCCCCGGTTTACTCAGTCTCTCCTAATTCCAGCCTCTCGAACAACTAATATCCTGTCTTTTCTATCCCTATGAAAAAAACTAACAGAGATCGATCTGTTTACGCGTGCCACCATGTTCGTGTTTCTGGTGCTGCTGCCTCTGGTGAGCTCCCAGTGCGTGAACCTGACCACAAGGACCCAGCTGCCCCCTGCCTATACCAATTCCTTCACACGGGGCGTGTACTATCCCGACAAGGTGTTCCGGAGCAGCGTGCTGCACTCCACACAGGATCTGTTTCTGCCTTTCTTTTCTAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAATGGCACAAAGCGGTTCGACAATCCAGTGCTGCCCTTTAACGATGGCGTGTACTTCGCCTCCACCGAGAAGTCTAACATCATCAGAGGCTGGATCTTTGGCACCACACTGGACAGCAAGACACAGTCCCTGCTGATCGTGAACAATGCCACCAACGTGGTCATCAAGGTGTGCGAGTTCCAGTTTTGTAATGATCCATTCCTGGGCGTGTACTATCACAAGAACAATAAGTCTTGGATGGAGAGCGAGTTTCGCGTGTATTCCTCTGCCAACAATTGCACATTTGAGTACGTGTCCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAATTTCAAGAACCTGAGGGAGTTCGTGTTTAAGAATATCGATGGCTACTTCAAAATCTACTCCAAGCACACCCCAATCAACCTGGTGCGCGACCTGCCACAGGGCTTCTCTGCCCTGGAGCCACTGGTGGATCTGCCCATCGGCATCAACATCACCCGGTTTCAGACACTGCTGGCCCTGCACAGAAGCTACCTGACACCAGGCGACAGCTCCTCTGGATGGACCGCAGGAGCAGCAGCCTACTATGTGGGCTATCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAATGGCACCATCACAGACGCCGTGGATTGCGCCCTGGATCCCCTGTCTGAGACCAAGTGTACACTGAAGAGCTTTACCGTGGAGAAGGGCATCTATCAGACAAGCAATTTCAGGGTGCAGCCTACCGAGTCCATCGTGCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAAGATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGGAGGGCTTTAACTGTTATTTCCCTCTGCAGAGCTACGGCTTCCAGCCAACAAACGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACCTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGACTGACCGGAACAGGCGTGCTGACCGAGTCCAACAAGAAGTTCCTGCCTTTTCAGCAGTTCGGCAGGGACATCGCAGATACCACAGACGCCGTGCGCGACCCTCAGACCCTGGAGATCCTGGACATCACACCATGCTCCTTCGGCGGCGTGTCTGTGATCACACCAGGCACCAATACAAGCAACCAGGTGGCCGTGCTGTATCAGGACGTGAATTGTACCGAGGTGCCAGTGGCAATCCACGCAGATCAGCTGACCCCTACATGGCGGGTGTACTCTACCGGCAGCAACGTGTTCCAGACAAGAGCCGGATGCCTGATCGGAGCAGAGCACGTGAACAATAGCTATGAGTGCGACATCCCTATCGGCGCCGGCATCTGTGCCTCCTACCAGACCCAGACAAACTCCCCAAGGTCTGTGGGCGATACAGGCCTGTCCAAGAATCCAATCGAGCTGGTAGAGGGCTGGTTCAGCAGTTGGAAAAGCTCCATCGCCTCCTTTTTCTTTATCATCGGCCTGATCATCGGACTGTTCCTGGTGCTCCGCGTGGGTATCCACCTGTGCATCAAGCTGAAGCACACCAAGAAAAGACAGATTTATACAGACATCGAGATGAACCGCCTGGGAAAGggatccggctccggcgagggcaggggaagtctactaacatgcggggacgtggaggaaaatcccggccccATGAAGTGCCTTTTGTACTTAGCCTTTTTATTCATTGGGGTGAATTGCAAGTTCACCATAGTTTTTCCACACAACCAAAAAGGAAACTGGAAAAATGTTCCTTCTAATTACCATTATTGCCCGTCAAGCTCAGATTTAAATTGGCATAATGACTTAATAGGCACAGCCATACAAGTCAAAATGCCCAAGAGTCACAAGGCTATTCAAGCAGACGGTTGGATGTGTCATGCTTCCAAATGGGTCACTACTTGTGATTTCCGCTGGTATGGACCGAAGTATATAACACAGTCCATCCGATCCTTCACTCCATCTGTAGAACAATGCAAGGAAAGCATTGAACAAACGAAACAAGGAACTTGGCTGAATCCAGGCTTCCCTCCTCAAAGTTGTGGATATGCAACTGTGACGGATGCCGAAGCAGTGATTGTCCAGGTGACTCCTCACCATGTGCTGGTTGATGAATACACAGGAGAATGGGTTGATTCACAGTTCATCAACGGAAAATGCAGCAATTACATATGCCCCACTGTCCATAACTCTACAACCTGGCATTCTGACTATAAGGTCAAAGGGCTATGTGATTCTAACCTCATTTCCATGGACATCACCTTCTTCTCAGAGGACGGAGAGCTATCATCCCTGGGAAAGGAGGGCACAGGGTTCAGAAGTAACTACTTTGCTTATGAAACTGGAGGCAAGGCCTGCAAAATGCAATACTGCAAGCATTGGGGAGTCAGACTCCCATCAGGTGTCTGGTTCGAGATGGCTGATAAGGATCTCTTTGCTGCAGCCAGATTCCCTGAATGCCCAGAAGGGTCAAGTATCTCTGCTCCATCTCAGACCTCAGTGGATGTAAGTCTAATTCAGGACGTTGAGAGGATCTTGGATTATTCCCTCTGCCAAGAAACCTGGAGCAAAATCAGAGCGGGTCTTCCAATCTCTCCAGTGGATCTCAGCTATCTTGCTCCTAAAAACCCAGGAACCGGTCCTGCTTTCACCATAATCAATGGTACCCTAAAATACTTTGAGACCAGATACATCAGAGTCGATATTGCTGCTCCAATCCTCTCAAGAATGGTCGGAATGATCAGTGGAACTACCACAGAAAGGGAACTGTGGGATGACTGGGCACCATATGAAGACGTGGAAATTGGACCCAATGGAGTTCTGAGGACCAGTTCAGGATATAAGTTTCCTTTATACATGATTGGACATGGTATGTTGGACTCCGATCTTCATCTTAGCTCAAAGGCTCAGGTGTTCGAACATCCTCACATTCAAGACGCTGCTTCGCAACTTCCTGATGATGAGAGTTTATTTTTTGGTGATACTGGGCTATCCAAAAATCCAATCGAGCTTGTAGAAGGTTGGTTCAGTAGTTGGAAAAGCTCTATTGCCTCTTTTTTCTTTATCATAGGGTTAATCATTGGACTATTCTTGGTTCTCCGAGTTGGTATCCATCTTTGCATTAAATTAAAGCACACCAAGAAAAGACAGATTTATACAGACATAGAGATGAACCGACTTGGAAAGTAAgCTAGCCAGATTCTTCATGTTTGGACCAAATCAACTTGTGATACCATGCTCAAAGAGGCCTCAATTATATTTGAGTTTTTAATTTTTATGAAAAAAACTAACAGCAATCATGGAAGTCCACGATTTTGAGACCGACGAGTTCAATGATTTCAATGAAGATGACTATGCCACAAGAGAATTCCTGAATCCCGATGAGCGCATGACGTACTTGAATCATGCTGATTACAATTTGAATTCTCCTCTAATTAGTGATGATATTGACAATTTGATCAGGAAATTCAATTCTCTTCCGATTCCCTCGATGTGGGATAGTAAGAACTGGGATGGAGTTCTTGAGATGTTAACATCATGTCAAGCCAATCCCATCTCAACATCTCAGATGCATAAATGGATGGGAAGTTGGTTAATGTCTGATAATCATGATGCCAGTCAAGGGTATAGTTTTTTACATGAAGTGGACAAAGAGGCAGAAATAACATTTGACGTGGTGGAGACCTTCATCCGCGGCTGGGGCAACAAACCAATTGAATACATCAAAAAGGAAAGATGGACTGACTCATTCAAAATTCTCGCTTATTTGTGTCAAAAGTTTTTGGACTTACACAAGTTGACATTAATCTTAAATGCTGTCTCTGAGGTGGAATTGCTCAACTTGGCGAGGACTTTCAAAGGCAAAGTCAGAAGAAGTTCTCATGGAACGAACATATGCAGGATTAGGGTTCCCAGCTTGGGTCCTACTTTTATTTCAGAAGGATGGGCTTACTTCAAGAAACTTGATATTCTAATGGACCGAAACTTTCTGTTAATGGTCAAAGATGTGATTATAGGGAGGATGCAAACGGTGCTATCCATGGTATGTAGAATAGACAACCTGTTCTCAGAGCAAGACATCTTCTCCCTTCTAAATATCTACAGAATTGGAGATAAAATTGTGGAGAGGCAGGGAAATTTTTCTTATGACTTGATTAAAATGGTGGAACCGATATGCAACTTGAAGCTGATGAAATTAGCAAGAGAATCAAGGCCTTTAGTCCCACAATTCCCTCATTTTGAAAATCATATCAAGACTTCTGTTGATGAAGGGGCAAAAATTGACCGAGGTATAAGATTCCTCCATGATCAGATAATGAGTGTGAAAACAGTGGATCTCACACTGGTGATTTATGGATCGTTCAGACATTGGGGTCATCCTTTTATAGATTATTACACTGGACTAGAAAAATTACATTCCCAAGTAACCATGAAGAAAGATATTGATGTGTCATATGCAAAAGCACTTGCAAGTGATTTAGCTCGGATTGTTCTATTTCAACAGTTCAATGATCATAAAAAGTGGTTCGTGAATGGAGACTTGCTCCCTCATGATCATCCCTTTAAAAGTCATGTTAAAGAAAATACATGGCCCACAGCTGCTCAAGTTCAAGATTTTGGAGATAAATGGCATGAACTTCCGCTGATTAAATGTTTTGAAATACCCGACTTACTAGACCCATCGATAATATACTCTGACAAAAGTCATTCAATGAATAGGTCAGAGGTGTTGAAACATGTCCGAATGAATCCGAACACTCCTATCCCTAGTAAAAAGGTGTTGCAGACTATGTTGGACACAAAGGCTACCAATTGGAAAGAATTTCTTAAAGAGATTGATGAGAAGGGCTTAGATGATGATGATCTAATTATTGGTCTTAAAGGAAAGGAGAGGGAACTGAAGTTGGCAGGTAGATTTTTCTCCCTAATGTCTTGGAAATTGCGAGAATACTTTGTAATTACCGAATATTTGATAAAGACTCATTTCGTCCCTATGTTTAAAGGCCTGACAATGGCGGACGATCTAACTGCAGTCATTAAAAAGATGTTAGATTCCTCATCCGGCCAAGGATTGAAGTCATATGAGGCAATTTGCATAGCCAATCACATTGATTACGAAAAATGGAATAACCACCAAAGGAAGTTATCAAACGGCCCAGTGTTCCGAGTTATGGGCCAGTTCTTAGGTTATCCATCCTTAATCGAGAGAACTCATGAATTTTTTGAGAAAAGTCTTATATACTACAATGGAAGACCAGACTTGATGCGTGTTCACAACAACACACTGATCAATTCAACCTCCCAACGAGTTTGTTGGCAAGGACAAGAGGGTGGACTGGAAGGTCTACGGCAAAAAGGATGGACTATCCTCAATCTACTGGTTATTCAAAGAGAGGCTAAAATCAGAAACACTGCTGTCAAAGTCTTGGCACAAGGTGATAATCAAGTTATTTGCACACAGTATAAAACGAAGAAATCGAGAAACGTTGTAGAATTACAGGGTGCTCTCAATCAAATGGTTTCTAATAATGAGAAAATTATGACTGCAATCAAAATAGGGACAGGGAAGTTAGGACTTTTGATAAATGACGATGAGACTATGCAATCTGCAGATTACTTGAATTATGGAAAAATACCGATTTTCCGTGGAGTGATTAGAGGGTTAGAGACCAAGAGATGGTCACGAGTGACTTGTGTCACCAATGACCAAATACCCACTTGTGCTAATATAATGAGCTCAGTTTCCACAAATGCTCTCACCGTAGCTCATTTTGCTGAGAACCCAATCAATGCCATGATACAGTACAATTATTTTGGGACATTTGCTAGACTCTTGTTGATGATGCATGATCCTGCTCTTCGTCAATCATTGTATGAAGTTCAAGATAAGATACCGGGCTTGCACAGTTCTACTTTCAAATACGCCATGTTGTATTTGGACCCTTCCATTGGAGGAGTGTCGGGCATGTCTTTGTCCAGGTTTTTGATTAGAGCCTTCCCAGATCCCGTAACAGAAAGTCTCTCATTCTGGAGATTCATCCATGTACATGCTCGAAGTGAGCATCTGAAGGAGATGAGTGCAGTATTTGGAAACCCCGAGATAGCCAAGTTTCGAATAACTCACATAGACAAGCTAGTAGAAGATCCAACCTCTCTGAACATCGCTATGGGAATGAGTCCAGCGAACTTGTTAAAGACTGAGGTTAAAAAATGCTTAATCGAATCAAGACAAACCATCAGGAACCAGGTGATTAAGGATGCAACCATATATTTGTATCATGAAGAGGATCGGCTCAGAAGTTTCTTATGGTCAATAAATCCTCTGTTCCCTAGATTTTTAAGTGAATTCAAATCAGGCACTTTTTTGGGAGTCGCAGACGGGCTCATCAGTCTATTTCAAAATTCTCGTACTATTCGGAACTCCTTTAAGAAAAAGTATCATAGGGAATTGGATGATTTGATTGTGAGGAGTGAGGTATCCTCTTTGACACATTTAGGGAAACTTCATTTGAGAAGGGGATCATGTAAAATGTGGACATGTTCAGCTACTCATGCTGACACATTAAGATACAAATCCTGGGGCCGTACAGTTATTGGGACAACTGTACCCCATCCATTAGAAATGTTGGGTCCACAACATCGAAAAGAGACTCCTTGTGCACCATGTAACACATCAGGGTTCAATTATGTTTCTGTGCATTGTCCAGACGGGATCCATGACGTCTTTAGTTCACGGGGACCATTGCCTGCTTATCTAGGGTCTAAAACATCTGAATCTACATCTATTTTGCAGCCTTGGGAAAGGGAAAGCAAAGTCCCACTGATTAAAAGAGCTACACGTCTTAGAGATGCTATCTCTTGGTTTGTTGAACCCGACTCTAAACTAGCAATGACTATACTTTCTAACATCCACTCTTTAACAGGCGAAGAATGGACCAAAAGGCAGCATGGGTTCAAAAGAACAGGGTCTGCCCTTCATAGGTTTTCGACATCTCGGATGAGCCATGGTGGGTTCGCATCTCAGAGCACTGCAGCATTGACCAGGTTGATGGCAACTACAGACACCATGAGGGATCTGGGAGATCAGAATTTCGACTTTTTATTCCAAGCAACGTTGCTCTATGCTCAAATTACCACCACTGTTGCAAGAGACGGATGGATCACCAGTTGTACAGATCATTATCATATTGCCTGTAAGTCCTGTTTGAGACCCATAGAAGAGATCACCCTGGACTCAAGTATGGACTACACGCCCCCAGATGTATCCCATGTGCTGAAGACATGGAGGAATGGGGAAGGTTCGTGGGGACAAGAGATAAAACAGATCTATCCTTTAGAAGGGAATTGGAAGAATTTAGCACCTGCTGAGCAATCCTATCAAGTCGGCAGATGTATAGGTTTTCTATATGGAGACTTGGCGTATAGAAAATCTACTCATGCCGAGGACAGTTCTCTATTTCCTCTATCTATACAAGGTCGTATTAGAGGTCGAGGTTTCTTAAAAGGGTTGCTAGACGGATTAATGAGAGCAAGTTGCTGCCAAGTAATACACCGGAGAAGTCTGGCTCATTTGAAGAGGCCGGCCAACGCAGTGTACGGAGGTTTGATTTACTTGATTGATAAATTGAGTGTATCACCTCCATTCCTTTCTCTTACTAGATCAGGACCTATTAGAGACGAATTAGAAACGATTCCCCACAAGATCCCAACCTCCTATCCGACAAGCAACCGTGATATGGGGGTGATTGTCAGAAATTACTTCAAATACCAATGCCGTCTAATTGAAAAGGGAAAATACAGATCACATTATTCACAATTATGGTTATTCTCAGATGTCTTATCCATAGACTTCATTGGACCATTCTCTATTTCCACCACCCTCTTGCAAATCCTATACAAGCCATTTTTATCTGGGAAAGATAAGAATGAGTTGAGAGAGCTGGCAAATCTTTCTTCATTGCTAAGATCAGGAGAGGGGTGGGAAGACATACATGTGAAATTCTTCACCAAGGACATATTATTGTGTCCAGAGGAAATCAGACATGCTTGCAAGTTCGGGATTGCTAAGGATAATAATAAAGACATGAGCTATCCCCCTTGGGGAAGGGAATCCAGAGGGACAATTACAACAATCCCTGTTTATTATACGACCACCCCTTACCCAAAGATGCTAGAGATGCCTCCAAGAATCCAAAATCCCCTGCTGTCCGGAATCAGGTTGGGCCAATTACCAACTGGCGCTCATTATAAAATTCGGAGTATATTACATGGAATGGGAATCCATTACAGGGACTTCTTGAGTTGTGGAGACGGCTCCGGAGGGATGACTGCTGCATTACTACGAGAAAATGTGCATAGCAGAGGAATATTCAATAGTCTGTTAGAATTATCAGGGTCAGTCATGCGAGGCGCCTCTCCTGAGCCCCCCAGTGCCCTAGAAACTTTAGGAGGAGATAAATCGAGATGTGTAAATGGTGAAACATGTTGGGAATATCCATCTGACTTATGTGACCCAAGGACTTGGGACTATTTCCTCCGACTCAAAGCAGGCTTGGGGCTTCAAATTGATTTAATTGTAATGGATATGGAAGTTCGGGATTCTTCTACTAGCCTGAAAATTGAGACGAATGTTAGAAATTATGTGCACCGGATTTTGGATGAGCAAGGAGTTTTAATCTACAAGACTTATGGAACATATATTTGTGAGAGCGAAAAGAATGCAGTAACAATCCTTGGTCCCATGTTCAAGACGGTCGACTTAGTTCAAACAGAATTTAGTAGTTCTCAAACGTCTGAAGTATATATGGTATGTAAAGGTTTGAAGAAATTAATCGATGAACCCAATCCCGATTGGTCTTCCATCAATGAATCCTGGAAAAACCTGTACGCATTCCAGTCATCAGAACAGGAATTTGCCAGAGCAAAGAAGGTTAGTACATACTTTACCTTGACAGGTATTCCCTCCCAATTCATTCCTGATCCTTTTGTAAACATTGAGACTATGCTACAAATATTCGGAGTACCCACGGGTGTGTCTCATGCGGCTGCCTTAAAATCATCTGATAGACCTGCAGATTTATTGACCATTAGCCTTTTTTATATGGCGATTATATCGTATTATAACATCAATCATATCAGAGTAGGACCGATACCTCCGAACCCCCCATCAGATGGAATTGCACAAAATGTGGGGATCGCTATAACTGGTATAAGCTTTTGGCTGAGTTTGATGGAGAAAGACATTCCACTATATCAACAGTGTTTAGCAGTTATCCAGCAATCATTCCCGATTAGGTGGGAGGCTGTTTCAGTAAAAGGAGGATACAAGCAGAAGTGGAGTACTAGAGGTGATGGGCTCCCAAAAGATACCCGAACTTCAGACTCCTTGGCCCCAATCGGGAACTGGATCAGATCTCTGGAATTGGTCCGAAACCAAGTTCGTCTAAATCCATTCAATGAGATCTTGTTCAATCAGCTATGTCGTACAGTGGATAATCATTTGAAATGGTCAAATTTGCGAAGAAACACAGGAATGATTGAATGGATCAATAGACGAATTTCAAAAGAAGACCGGTCTATACTGATGTTGAAGAGTGACCTACACGAGGAAAACTCTTGGAGAGATTAAAAAATCATGAGGAGACTCCAAACTTTAAGTATGAAAAAAACTTTGATCCTTAAGACCCTCTTGTGGTTTTTATTTTTTATCTGGTTTTGTGGTCTTCGT。
在其他实施方式中，核酸包括SEQ ID NO：25的序列，如下所示(VSV载体：ConvacV4 South Africa)：
ACGAAGACAAACAAACCATTATTATCATTAAAAGGCTCAGGAGAAACTTTAACAGTAATCAAAATGTCTGTTACAGTCAAGAGAATCATTGACAACACAGTCATAGTTCCAAAACTTCCTGCAAATGAGGATCCAGTGGAATACCCGGCAGATTACTTCAGAAAATCAAAGGAGATTCCTCTTTACATCAATACTACAAAAAGTTTGTCAGATCTAAGAGGATATGTCTACCAAGGCCTCAAATCCGGAAATGTATCAATCATACATGTCAACAGCTACTTGTATGGAGCATTAAAGGACATCCGGGGTAAGTTGGATAAAGATTGGTCAAGTTTCGGAATAAACATCGGGAAAGCAGGGGATACAATCGGAATATTTGACCTTGTATCCTTGAAAGCCCTGGACGGCGTACTTCCAGATGGAGTATCGGATGCTTCCAGAACCAGCGCAGATGACAAATGGTTGCCTTTGTATCTACTTGGCTTATACAGAGTGGGCAGAACACAAATGCCTGAATACAGAAAAAAGCTCATGGATGGGCTGACAAATCAATGCAAAATGATCAATGAACAGTTTGAACCTCTTGTGCCAGAAGGTCGTGACATTTTTGATGTGTGGGGAAATGACAGTAATTACACAAAAATTGTCGCTGCAGTGGACATGTTCTTCCACATGTTCAAAAAACATGAATGTGCCTCGTTCAGATACGGAACTATTGTTTCCAGATTCAAAGATTGTGCTGCATTGGCAACATTTGGACACCTCTGCAAAATAACCGGAATGTCTACAGAAGATGTAACGACCTGGATCTTGAACCGAGAAGTTGCAGATGAAATGGTCCAAATGATGCTTCCAGGCCAAGAAATTGACAAGGCCGATTCATACATGCCTTATTTGATCGACTTTGGATTGTCTTCTAAGTCTCCATATTCTTCCGTCAAAAACCCTGCCTTCCACTTCTGGGGGCAATTGACAGCTCTTCTGCTCAGATCCACCAGAGCAAGGAATGCCCGACAGCCTGATGACATTGAGTATACATCTCTTACTACAGCAGGTTTGTTGTACGCTTATGCAGTAGGATCCTCTGCCGACTTGGCACAACAGTTTTGTGTTGGAGATAACAAATACACTCCAGATGATAGTACCGGAGGATTGACGACTAATGCACCGCCACAAGGCAGAGATGTGGTCGAATGGCTCGGATGGTTTGAAGATCAAAACAGAAAACCGACTCCTGATATGATGCAGTATGCGAAAAGAGCAGTCATGTCACTGCAAGGCCTAAGAGAGAAGACAATTGGCAAGTATGCTAAGTCAGAATTTGACAAATGACCCTATAATTCTCAGATCACCTATTATATATTATGCTACATATGAAAAAAACTAACAGATATCATGGATAATCTCACAAAAGTTCGTGAGTATCTCAAGTCCTATTCTCGTCTGGATCAGGCGGTAGGAGAGATAGATGAGATCGAAGCACAACGAGCTGAAAAGTCCAATTATGAGTTGTTCCAAGAGGATGGAGTGGAAGAGCATACTAAGCCCTCTTATTTTCAGGCAGCAGATGATTCTGACACAGAATCTGAACCAGAAATTGAAGACAATCAAGGTTTGTATGCACCAGATCCAGAAGCTGAGCAAGTTGAAGGCTTTATACAGGGGCCTTTAGATGACTATGCAGATGAGGAAGTGGATGTTGTATTTACTTCGGACTGGAAACAGCCTGAGCTTGAATCTGACGAGCATGGAAAGACCTTACGGTTGACATCGCCAGAGGGTTTAAGTGGAGAGCAGAAATCCCAGTGGCTTTCGACGATTAAAGCAGTCGTGCAAAGTGCCAAATACTGGAATCTGGCAGAGTGCACATTTGAAGCATCGGGAGAAGGGGTCATTATGAAGGAGCGCCAGATAACTCCGGATGTATATAAGGTCACTCCAGTGATGAACACACATCCGTCCCAATCAGAAGCAGTATCAGATGTTTGGTCTCTCTCAAAGACATCCATGACTTTCCAACCCAAGAAAGCAAGTCTTCAGCCTCTCACCATATCCTTGGATGAATTGTTCTCATCTAGAGGAGAGTTCATCTCTGTCGGAGGTGACGGACGAATGTCTCATAAAGAGGCCATCCTGCTCGGCCTGAGATACAAAAAGTTGTACAATCAGGCGAGAGTCAAATATTCTCTGTAGACTATGAAAAAAAGTAACAGATATCACGATCTAAGTGTTATCCCAATCCATTCATCATGAGTTCCTTAAAGAAGATTCTCGGTCTGAAGGGGAAAGGTAAGAAATCTAAGAAATTAGGGATCGCACCACCCCCTTATGAAGAGGACACTAGCATGGAGTATGCTCCGAGCGCTCCAATTGACAAATCCTATTTTGGAGTTGACGAGATGGACACCTATGATCCGAATCAATTAAGATATGAGAAATTCTTCTTTACAGTGAAAATGACGGTTAGATCTAATCGTCCGTTCAGAACATACTCAGATGTGGCAGCCGCTGTATCCCATTGGGATCACATGTACATCGGAATGGCAGGGAAACGTCCCTTCTACAAAATCTTGGCTTTTTTGGGTTCTTCTAATCTAAAGGCCACTCCAGCGGTATTGGCAGATCAAGGTCAACCAGAGTATCACACTCACTGCGAAGGCAGGGCTTATTTGCCACATAGGATGGGGAAGACCCCTCCCATGCTCAATGTACCAGAGCACTTCAGAAGACCATTCAATATAGGTCTTTACAAGGGAACGATTGAGCTCACAATGACCATCTACGATGATGAGTCACTGGAAGCAGCTCCTATGATCTGGGATCATTTCAATTCTTCCAAATTTTCTGATTTCAGAGAGAAGGCCTTAATGTTTGGCCTGATTGTCGAGAAAAAGGCATCTGGAGCGTGGGTCCTGGATTCTATCAGCCACTTCAAATGAGCTAGTCTAACTTCTAGCTTCTGAACAATCCCCGGTTTACTCAGTCTCTCCTAATTCCAGCCTCTCGAACAACTAATATCCTGTCTTTTCTATCCCTATGAAAAAAACTAACAGAGATCGATCTGTTTACGCGTGCCACCATGTTCGTGTTTCTGGTGCTGCTGCCTCTGGTGAGCTCCCAGTGCGTGAACTTCACCACAAGGACCCAGCTGCCCCCTGCCTATACCAATTCCTTCACACGGGGCGTGTACTATCCCGACAAGGTGTTCCGGAGCAGCGTGCTGCACTCCACACAGGATCTGTTTCTGCCTTTCTTTTCTAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAATGGCACAAAGCGGTTCGCCAATCCAGTGCTGCCCTTTAACGATGGCGTGTACTTCGCCTCCACCGAGAAGTCTAACATCATCAGAGGCTGGATCTTTGGCACCACACTGGACAGCAAGACACAGTCCCTGCTGATCGTGAACAATGCCACCAACGTGGTCATCAAGGTGTGCGAGTTCCAGTTTTGTAATGATCCATTCCTGGGCGTGTACTATCACAAGAACAATAAGTCTTGGATGGAGAGCGAGTTTCGCGTGTATTCCTCTGCCAACAATTGCACATTTGAGTACGTGTCCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAATTTCAAGAACCTGAGGGAGTTCGTGTTTAAGAATATCGATGGCTACTTCAAAATCTACTCCAAGCACACCCCAATCAACCTGGTGCGCGGCCTGCCACAGGGCTTCTCTGCCCTGGAGCCACTGGTGGATCTGCCCATCGGCATCAACATCACCCGGTTTCAGACACTGCTGGCCCTGCACAGAAGCTACCTGACACCAGGCGACAGCTCCTCTGGATGGACCGCAGGAGCAGCAGCCTACTATGTGGGCTATCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAATGGCACCATCACAGACGCCGTGGATTGCGCCCTGGATCCCCTGTCTGAGACCAAGTGTACACTGAAGAGCTTTACCGTGGAGAAGGGCATCTATCAGACAAGCAATTTCAGGGTGCAGCCTACCGAGTCCATCGTGCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAATATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGAAGGGCTTTAACTGTTATTTCCCTCTGCAGAGCTACGGCTTCCAGCCAACATATGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACCTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGACTGACCGGAACAGGCGTGCTGACCGAGTCCAACAAGAAGTTCCTGCCTTTTCAGCAGTTCGGCAGGGACATCGCAGATACCACAGACGCCGTGCGCGACCCTCAGACCCTGGAGATCCTGGACATCACACCATGCTCCTTCGGCGGCGTGTCTGTGATCACACCAGGCACCAATACAAGCAACCAGGTGGCCGTGCTGTATCAGGGCGTGAATTGTACCGAGGTGCCAGTGGCAATCCACGCAGATCAGCTGACCCCTACATGGCGGGTGTACTCTACCGGCAGCAACGTGTTCCAGACAAGAGCCGGATGCCTGATCGGAGCAGAGCACGTGAACAATAGCTATGAGTGCGACATCCCTATCGGCGCCGGCATCTGTGCCTCCTACCAGACCCAGACAAACTCCCCAAGGTCTGTGGGCGATACAGGCCTGTCCAAGAATCCAATCGAGCTGGTAGAGGGCTGGTTCAGCAGTTGGAAAAGCTCCATCGCCTCCTTTTTCTTTATCATCGGCCTGATCATCGGACTGTTCCTGGTGCTCCGCGTGGGTATCCACCTGTGCATCAAGCTGAAGCACACCAAGAAAAGACAGATTTATACAGACATCGAGATGAACCGCCTGGGAAAGggatccggctccggcgagggcaggggaagtctactaacatgcggggacgtggaggaaaatcccggccccATGAAGTGCCTTTTGTACTTAGCCTTTTTATTCATTGGGGTGAATTGCAAGTTCACCATAGTTTTTCCACACAACCAAAAAGGAAACTGGAAAAATGTTCCTTCTAATTACCATTATTGCCCGTCAAGCTCAGATTTAAATTGGCATAATGACTTAATAGGCACAGCCATACAAGTCAAAATGCCCAAGAGTCACAAGGCTATTCAAGCAGACGGTTGGATGTGTCATGCTTCCAAATGGGTCACTACTTGTGATTTCCGCTGGTATGGACCGAAGTATATAACACAGTCCATCCGATCCTTCACTCCATCTGTAGAACAATGCAAGGAAAGCATTGAACAAACGAAACAAGGAACTTGGCTGAATCCAGGCTTCCCTCCTCAAAGTTGTGGATATGCAACTGTGACGGATGCCGAAGCAGTGATTGTCCAGGTGACTCCTCACCATGTGCTGGTTGATGAATACACAGGAGAATGGGTTGATTCACAGTTCATCAACGGAAAATGCAGCAATTACATATGCCCCACTGTCCATAACTCTACAACCTGGCATTCTGACTATAAGGTCAAAGGGCTATGTGATTCTAACCTCATTTCCATGGACATCACCTTCTTCTCAGAGGACGGAGAGCTATCATCCCTGGGAAAGGAGGGCACAGGGTTCAGAAGTAACTACTTTGCTTATGAAACTGGAGGCAAGGCCTGCAAAATGCAATACTGCAAGCATTGGGGAGTCAGACTCCCATCAGGTGTCTGGTTCGAGATGGCTGATAAGGATCTCTTTGCTGCAGCCAGATTCCCTGAATGCCCAGAAGGGTCAAGTATCTCTGCTCCATCTCAGACCTCAGTGGATGTAAGTCTAATTCAGGACGTTGAGAGGATCTTGGATTATTCCCTCTGCCAAGAAACCTGGAGCAAAATCAGAGCGGGTCTTCCAATCTCTCCAGTGGATCTCAGCTATCTTGCTCCTAAAAACCCAGGAACCGGTCCTGCTTTCACCATAATCAATGGTACCCTAAAATACTTTGAGACCAGATACATCAGAGTCGATATTGCTGCTCCAATCCTCTCAAGAATGGTCGGAATGATCAGTGGAACTACCACAGAAAGGGAACTGTGGGATGACTGGGCACCATATGAAGACGTGGAAATTGGACCCAATGGAGTTCTGAGGACCAGTTCAGGATATAAGTTTCCTTTATACATGATTGGACATGGTATGTTGGACTCCGATCTTCATCTTAGCTCAAAGGCTCAGGTGTTCGAACATCCTCACATTCAAGACGCTGCTTCGCAACTTCCTGATGATGAGAGTTTATTTTTTGGTGATACTGGGCTATCCAAAAATCCAATCGAGCTTGTAGAAGGTTGGTTCAGTAGTTGGAAAAGCTCTATTGCCTCTTTTTTCTTTATCATAGGGTTAATCATTGGACTATTCTTGGTTCTCCGAGTTGGTATCCATCTTTGCATTAAATTAAAGCACACCAAGAAAAGACAGATTTATACAGACATAGAGATGAACCGACTTGGAAAGTAAgCTAGCCAGATTCTTCATGTTTGGACCAAATCAACTTGTGATACCATGCTCAAAGAGGCCTCAATTATATTTGAGTTTTTAATTTTTATGAAAAAAACTAACAGCAATCATGGAAGTCCACGATTTTGAGACCGACGAGTTCAATGATTTCAATGAAGATGACTATGCCACAAGAGAATTCCTGAATCCCGATGAGCGCATGACGTACTTGAATCATGCTGATTACAATTTGAATTCTCCTCTAATTAGTGATGATATTGACAATTTGATCAGGAAATTCAATTCTCTTCCGATTCCCTCGATGTGGGATAGTAAGAACTGGGATGGAGTTCTTGAGATGTTAACATCATGTCAAGCCAATCCCATCTCAACATCTCAGATGCATAAATGGATGGGAAGTTGGTTAATGTCTGATAATCATGATGCCAGTCAAGGGTATAGTTTTTTACATGAAGTGGACAAAGAGGCAGAAATAACATTTGACGTGGTGGAGACCTTCATCCGCGGCTGGGGCAACAAACCAATTGAATACATCAAAAAGGAAAGATGGACTGACTCATTCAAAATTCTCGCTTATTTGTGTCAAAAGTTTTTGGACTTACACAAGTTGACATTAATCTTAAATGCTGTCTCTGAGGTGGAATTGCTCAACTTGGCGAGGACTTTCAAAGGCAAAGTCAGAAGAAGTTCTCATGGAACGAACATATGCAGGATTAGGGTTCCCAGCTTGGGTCCTACTTTTATTTCAGAAGGATGGGCTTACTTCAAGAAACTTGATATTCTAATGGACCGAAACTTTCTGTTAATGGTCAAAGATGTGATTATAGGGAGGATGCAAACGGTGCTATCCATGGTATGTAGAATAGACAACCTGTTCTCAGAGCAAGACATCTTCTCCCTTCTAAATATCTACAGAATTGGAGATAAAATTGTGGAGAGGCAGGGAAATTTTTCTTATGACTTGATTAAAATGGTGGAACCGATATGCAACTTGAAGCTGATGAAATTAGCAAGAGAATCAAGGCCTTTAGTCCCACAATTCCCTCATTTTGAAAATCATATCAAGACTTCTGTTGATGAAGGGGCAAAAATTGACCGAGGTATAAGATTCCTCCATGATCAGATAATGAGTGTGAAAACAGTGGATCTCACACTGGTGATTTATGGATCGTTCAGACATTGGGGTCATCCTTTTATAGATTATTACACTGGACTAGAAAAATTACATTCCCAAGTAACCATGAAGAAAGATATTGATGTGTCATATGCAAAAGCACTTGCAAGTGATTTAGCTCGGATTGTTCTATTTCAACAGTTCAATGATCATAAAAAGTGGTTCGTGAATGGAGACTTGCTCCCTCATGATCATCCCTTTAAAAGTCATGTTAAAGAAAATACATGGCCCACAGCTGCTCAAGTTCAAGATTTTGGAGATAAATGGCATGAACTTCCGCTGATTAAATGTTTTGAAATACCCGACTTACTAGACCCATCGATAATATACTCTGACAAAAGTCATTCAATGAATAGGTCAGAGGTGTTGAAACATGTCCGAATGAATCCGAACACTCCTATCCCTAGTAAAAAGGTGTTGCAGACTATGTTGGACACAAAGGCTACCAATTGGAAAGAATTTCTTAAAGAGATTGATGAGAAGGGCTTAGATGATGATGATCTAATTATTGGTCTTAAAGGAAAGGAGAGGGAACTGAAGTTGGCAGGTAGATTTTTCTCCCTAATGTCTTGGAAATTGCGAGAATACTTTGTAATTACCGAATATTTGATAAAGACTCATTTCGTCCCTATGTTTAAAGGCCTGACAATGGCGGACGATCTAACTGCAGTCATTAAAAAGATGTTAGATTCCTCATCCGGCCAAGGATTGAAGTCATATGAGGCAATTTGCATAGCCAATCACATTGATTACGAAAAATGGAATAACCACCAAAGGAAGTTATCAAACGGCCCAGTGTTCCGAGTTATGGGCCAGTTCTTAGGTTATCCATCCTTAATCGAGAGAACTCATGAATTTTTTGAGAAAAGTCTTATATACTACAATGGAAGACCAGACTTGATGCGTGTTCACAACAACACACTGATCAATTCAACCTCCCAACGAGTTTGTTGGCAAGGACAAGAGGGTGGACTGGAAGGTCTACGGCAAAAAGGATGGACTATCCTCAATCTACTGGTTATTCAAAGAGAGGCTAAAATCAGAAACACTGCTGTCAAAGTCTTGGCACAAGGTGATAATCAAGTTATTTGCACACAGTATAAAACGAAGAAATCGAGAAACGTTGTAGAATTACAGGGTGCTCTCAATCAAATGGTTTCTAATAATGAGAAAATTATGACTGCAATCAAAATAGGGACAGGGAAGTTAGGACTTTTGATAAATGACGATGAGACTATGCAATCTGCAGATTACTTGAATTATGGAAAAATACCGATTTTCCGTGGAGTGATTAGAGGGTTAGAGACCAAGAGATGGTCACGAGTGACTTGTGTCACCAATGACCAAATACCCACTTGTGCTAATATAATGAGCTCAGTTTCCACAAATGCTCTCACCGTAGCTCATTTTGCTGAGAACCCAATCAATGCCATGATACAGTACAATTATTTTGGGACATTTGCTAGACTCTTGTTGATGATGCATGATCCTGCTCTTCGTCAATCATTGTATGAAGTTCAAGATAAGATACCGGGCTTGCACAGTTCTACTTTCAAATACGCCATGTTGTATTTGGACCCTTCCATTGGAGGAGTGTCGGGCATGTCTTTGTCCAGGTTTTTGATTAGAGCCTTCCCAGATCCCGTAACAGAAAGTCTCTCATTCTGGAGATTCATCCATGTACATGCTCGAAGTGAGCATCTGAAGGAGATGAGTGCAGTATTTGGAAACCCCGAGATAGCCAAGTTTCGAATAACTCACATAGACAAGCTAGTAGAAGATCCAACCTCTCTGAACATCGCTATGGGAATGAGTCCAGCGAACTTGTTAAAGACTGAGGTTAAAAAATGCTTAATCGAATCAAGACAAACCATCAGGAACCAGGTGATTAAGGATGCAACCATATATTTGTATCATGAAGAGGATCGGCTCAGAAGTTTCTTATGGTCAATAAATCCTCTGTTCCCTAGATTTTTAAGTGAATTCAAATCAGGCACTTTTTTGGGAGTCGCAGACGGGCTCATCAGTCTATTTCAAAATTCTCGTACTATTCGGAACTCCTTTAAGAAAAAGTATCATAGGGAATTGGATGATTTGATTGTGAGGAGTGAGGTATCCTCTTTGACACATTTAGGGAAACTTCATTTGAGAAGGGGATCATGTAAAATGTGGACATGTTCAGCTACTCATGCTGACACATTAAGATACAAATCCTGGGGCCGTACAGTTATTGGGACAACTGTACCCCATCCATTAGAAATGTTGGGTCCACAACATCGAAAAGAGACTCCTTGTGCACCATGTAACACATCAGGGTTCAATTATGTTTCTGTGCATTGTCCAGACGGGATCCATGACGTCTTTAGTTCACGGGGACCATTGCCTGCTTATCTAGGGTCTAAAACATCTGAATCTACATCTATTTTGCAGCCTTGGGAAAGGGAAAGCAAAGTCCCACTGATTAAAAGAGCTACACGTCTTAGAGATGCTATCTCTTGGTTTGTTGAACCCGACTCTAAACTAGCAATGACTATACTTTCTAACATCCACTCTTTAACAGGCGAAGAATGGACCAAAAGGCAGCATGGGTTCAAAAGAACAGGGTCTGCCCTTCATAGGTTTTCGACATCTCGGATGAGCCATGGTGGGTTCGCATCTCAGAGCACTGCAGCATTGACCAGGTTGATGGCAACTACAGACACCATGAGGGATCTGGGAGATCAGAATTTCGACTTTTTATTCCAAGCAACGTTGCTCTATGCTCAAATTACCACCACTGTTGCAAGAGACGGATGGATCACCAGTTGTACAGATCATTATCATATTGCCTGTAAGTCCTGTTTGAGACCCATAGAAGAGATCACCCTGGACTCAAGTATGGACTACACGCCCCCAGATGTATCCCATGTGCTGAAGACATGGAGGAATGGGGAAGGTTCGTGGGGACAAGAGATAAAACAGATCTATCCTTTAGAAGGGAATTGGAAGAATTTAGCACCTGCTGAGCAATCCTATCAAGTCGGCAGATGTATAGGTTTTCTATATGGAGACTTGGCGTATAGAAAATCTACTCATGCCGAGGACAGTTCTCTATTTCCTCTATCTATACAAGGTCGTATTAGAGGTCGAGGTTTCTTAAAAGGGTTGCTAGACGGATTAATGAGAGCAAGTTGCTGCCAAGTAATACACCGGAGAAGTCTGGCTCATTTGAAGAGGCCGGCCAACGCAGTGTACGGAGGTTTGATTTACTTGATTGATAAATTGAGTGTATCACCTCCATTCCTTTCTCTTACTAGATCAGGACCTATTAGAGACGAATTAGAAACGATTCCCCACAAGATCCCAACCTCCTATCCGACAAGCAACCGTGATATGGGGGTGATTGTCAGAAATTACTTCAAATACCAATGCCGTCTAATTGAAAAGGGAAAATACAGATCACATTATTCACAATTATGGTTATTCTCAGATGTCTTATCCATAGACTTCATTGGACCATTCTCTATTTCCACCACCCTCTTGCAAATCCTATACAAGCCATTTTTATCTGGGAAAGATAAGAATGAGTTGAGAGAGCTGGCAAATCTTTCTTCATTGCTAAGATCAGGAGAGGGGTGGGAAGACATACATGTGAAATTCTTCACCAAGGACATATTATTGTGTCCAGAGGAAATCAGACATGCTTGCAAGTTCGGGATTGCTAAGGATAATAATAAAGACATGAGCTATCCCCCTTGGGGAAGGGAATCCAGAGGGACAATTACAACAATCCCTGTTTATTATACGACCACCCCTTACCCAAAGATGCTAGAGATGCCTCCAAGAATCCAAAATCCCCTGCTGTCCGGAATCAGGTTGGGCCAATTACCAACTGGCGCTCATTATAAAATTCGGAGTATATTACATGGAATGGGAATCCATTACAGGGACTTCTTGAGTTGTGGAGACGGCTCCGGAGGGATGACTGCTGCATTACTACGAGAAAATGTGCATAGCAGAGGAATATTCAATAGTCTGTTAGAATTATCAGGGTCAGTCATGCGAGGCGCCTCTCCTGAGCCCCCCAGTGCCCTAGAAACTTTAGGAGGAGATAAATCGAGATGTGTAAATGGTGAAACATGTTGGGAATATCCATCTGACTTATGTGACCCAAGGACTTGGGACTATTTCCTCCGACTCAAAGCAGGCTTGGGGCTTCAAATTGATTTAATTGTAATGGATATGGAAGTTCGGGATTCTTCTACTAGCCTGAAAATTGAGACGAATGTTAGAAATTATGTGCACCGGATTTTGGATGAGCAAGGAGTTTTAATCTACAAGACTTATGGAACATATATTTGTGAGAGCGAAAAGAATGCAGTAACAATCCTTGGTCCCATGTTCAAGACGGTCGACTTAGTTCAAACAGAATTTAGTAGTTCTCAAACGTCTGAAGTATATATGGTATGTAAAGGTTTGAAGAAATTAATCGATGAACCCAATCCCGATTGGTCTTCCATCAATGAATCCTGGAAAAACCTGTACGCATTCCAGTCATCAGAACAGGAATTTGCCAGAGCAAAGAAGGTTAGTACATACTTTACCTTGACAGGTATTCCCTCCCAATTCATTCCTGATCCTTTTGTAAACATTGAGACTATGCTACAAATATTCGGAGTACCCACGGGTGTGTCTCATGCGGCTGCCTTAAAATCATCTGATAGACCTGCAGATTTATTGACCATTAGCCTTTTTTATATGGCGATTATATCGTATTATAACATCAATCATATCAGAGTAGGACCGATACCTCCGAACCCCCCATCAGATGGAATTGCACAAAATGTGGGGATCGCTATAACTGGTATAAGCTTTTGGCTGAGTTTGATGGAGAAAGACATTCCACTATATCAACAGTGTTTAGCAGTTATCCAGCAATCATTCCCGATTAGGTGGGAGGCTGTTTCAGTAAAAGGAGGATACAAGCAGAAGTGGAGTACTAGAGGTGATGGGCTCCCAAAAGATACCCGAACTTCAGACTCCTTGGCCCCAATCGGGAACTGGATCAGATCTCTGGAATTGGTCCGAAACCAAGTTCGTCTAAATCCATTCAATGAGATCTTGTTCAATCAGCTATGTCGTACAGTGGATAATCATTTGAAATGGTCAAATTTGCGAAGAAACACAGGAATGATTGAATGGATCAATAGACGAATTTCAAAAGAAGACCGGTCTATACTGATGTTGAAGAGTGACCTACACGAGGAAAACTCTTGGAGAGATTAAAAAATCATGAGGAGACTCCAAACTTTAAGTATGAAAAAAACTTTGATCCTTAAGACCCTCTTGTGGTTTTTATTTTTTATCTGGTTTTGTGGTCTTCGT。
在一个实施方式中，核酸包括SEQ ID NO：26的序列，如下所示(VSV载体：ConvacV5 China)：
ACGAAGACAAACAAACCATTATTATCATTAAAAGGCTCAGGAGAAACTTTAACAGTAATCAAAATGTCTGTTACAGTCAAGAGAATCATTGACAACACAGTCATAGTTCCAAAACTTCCTGCAAATGAGGATCCAGTGGAATACCCGGCAGATTACTTCAGAAAATCAAAGGAGATTCCTCTTTACATCAATACTACAAAAAGTTTGTCAGATCTAAGAGGATATGTCTACCAAGGCCTCAAATCCGGAAATGTATCAATCATACATGTCAACAGCTACTTGTATGGAGCATTAAAGGACATCCGGGGTAAGTTGGATAAAGATTGGTCAAGTTTCGGAATAAACATCGGGAAAGCAGGGGATACAATCGGAATATTTGACCTTGTATCCTTGAAAGCCCTGGACGGCGTACTTCCAGATGGAGTATCGGATGCTTCCAGAACCAGCGCAGATGACAAATGGTTGCCTTTGTATCTACTTGGCTTATACAGAGTGGGCAGAACACAAATGCCTGAATACAGAAAAAAGCTCATGGATGGGCTGACAAATCAATGCAAAATGATCAATGAACAGTTTGAACCTCTTGTGCCAGAAGGTCGTGACATTTTTGATGTGTGGGGAAATGACAGTAATTACACAAAAATTGTCGCTGCAGTGGACATGTTCTTCCACATGTTCAAAAAACATGAATGTGCCTCGTTCAGATACGGAACTATTGTTTCCAGATTCAAAGATTGTGCTGCATTGGCAACATTTGGACACCTCTGCAAAATAACCGGAATGTCTACAGAAGATGTAACGACCTGGATCTTGAACCGAGAAGTTGCAGATGAAATGGTCCAAATGATGCTTCCAGGCCAAGAAATTGACAAGGCCGATTCATACATGCCTTATTTGATCGACTTTGGATTGTCTTCTAAGTCTCCATATTCTTCCGTCAAAAACCCTGCCTTCCACTTCTGGGGGCAATTGACAGCTCTTCTGCTCAGATCCACCAGAGCAAGGAATGCCCGACAGCCTGATGACATTGAGTATACATCTCTTACTACAGCAGGTTTGTTGTACGCTTATGCAGTAGGATCCTCTGCCGACTTGGCACAACAGTTTTGTGTTGGAGATAACAAATACACTCCAGATGATAGTACCGGAGGATTGACGACTAATGCACCGCCACAAGGCAGAGATGTGGTCGAATGGCTCGGATGGTTTGAAGATCAAAACAGAAAACCGACTCCTGATATGATGCAGTATGCGAAAAGAGCAGTCATGTCACTGCAAGGCCTAAGAGAGAAGACAATTGGCAAGTATGCTAAGTCAGAATTTGACAAATGACCCTATAATTCTCAGATCACCTATTATATATTATGCTACATATGAAAAAAACTAACAGATATCATGGATAATCTCACAAAAGTTCGTGAGTATCTCAAGTCCTATTCTCGTCTGGATCAGGCGGTAGGAGAGATAGATGAGATCGAAGCACAACGAGCTGAAAAGTCCAATTATGAGTTGTTCCAAGAGGATGGAGTGGAAGAGCATACTAAGCCCTCTTATTTTCAGGCAGCAGATGATTCTGACACAGAATCTGAACCAGAAATTGAAGACAATCAAGGTTTGTATGCACCAGATCCAGAAGCTGAGCAAGTTGAAGGCTTTATACAGGGGCCTTTAGATGACTATGCAGATGAGGAAGTGGATGTTGTATTTACTTCGGACTGGAAACAGCCTGAGCTTGAATCTGACGAGCATGGAAAGACCTTACGGTTGACATCGCCAGAGGGTTTAAGTGGAGAGCAGAAATCCCAGTGGCTTTCGACGATTAAAGCAGTCGTGCAAAGTGCCAAATACTGGAATCTGGCAGAGTGCACATTTGAAGCATCGGGAGAAGGGGTCATTATGAAGGAGCGCCAGATAACTCCGGATGTATATAAGGTCACTCCAGTGATGAACACACATCCGTCCCAATCAGAAGCAGTATCAGATGTTTGGTCTCTCTCAAAGACATCCATGACTTTCCAACCCAAGAAAGCAAGTCTTCAGCCTCTCACCATATCCTTGGATGAATTGTTCTCATCTAGAGGAGAGTTCATCTCTGTCGGAGGTGACGGACGAATGTCTCATAAAGAGGCCATCCTGCTCGGCCTGAGATACAAAAAGTTGTACAATCAGGCGAGAGTCAAATATTCTCTGTAGACTATGAAAAAAAGTAACAGATATCACGATCTAAGTGTTATCCCAATCCATTCATCATGAGTTCCTTAAAGAAGATTCTCGGTCTGAAGGGGAAAGGTAAGAAATCTAAGAAATTAGGGATCGCACCACCCCCTTATGAAGAGGACACTAGCATGGAGTATGCTCCGAGCGCTCCAATTGACAAATCCTATTTTGGAGTTGACGAGATGGACACCTATGATCCGAATCAATTAAGATATGAGAAATTCTTCTTTACAGTGAAAATGACGGTTAGATCTAATCGTCCGTTCAGAACATACTCAGATGTGGCAGCCGCTGTATCCCATTGGGATCACATGTACATCGGAATGGCAGGGAAACGTCCCTTCTACAAAATCTTGGCTTTTTTGGGTTCTTCTAATCTAAAGGCCACTCCAGCGGTATTGGCAGATCAAGGTCAACCAGAGTATCACACTCACTGCGAAGGCAGGGCTTATTTGCCACATAGGATGGGGAAGACCCCTCCCATGCTCAATGTACCAGAGCACTTCAGAAGACCATTCAATATAGGTCTTTACAAGGGAACGATTGAGCTCACAATGACCATCTACGATGATGAGTCACTGGAAGCAGCTCCTATGATCTGGGATCATTTCAATTCTTCCAAATTTTCTGATTTCAGAGAGAAGGCCTTAATGTTTGGCCTGATTGTCGAGAAAAAGGCATCTGGAGCGTGGGTCCTGGATTCTATCAGCCACTTCAAATGAGCTAGTCTAACTTCTAGCTTCTGAACAATCCCCGGTTTACTCAGTCTCTCCTAATTCCAGCCTCTCGAACAACTAATATCCTGTCTTTTCTATCCCTATGAAAAAAACTAACAGAGATCGATCTGTTTACGCGTCACTATGAAGTGCCTTTTGTACTTAGCCTTTTTATTCATTGGGGTGAATTGCAAGTTCACCATAGTTTTTCCACACAACCAAAAAGGAAACTGGAAAAATGTTCCTTCTAATTACCATTATTGCCCGTCAAGCTCAGATTTAAATTGGCATAATGACTTAATAGGCACAGCCATACAAGTCAAAATGCCCAAGAGTCACAAGGCTATTCAAGCAGACGGTTGGATGTGTCATGCTTCCAAATGGGTCACTACTTGTGATTTCCGCTGGTATGGACCGAAGTATATAACACAGTCCATCCGATCCTTCACTCCATCTGTAGAACAATGCAAGGAAAGCATTGAACAAACGAAACAAGGAACTTGGCTGAATCCAGGCTTCCCTCCTCAAAGTTGTGGATATGCAACTGTGACGGATGCCGAAGCAGTGATTGTCCAGGTGACTCCTCACCATGTGCTGGTTGATGAATACACAGGAGAATGGGTTGATTCACAGTTCATCAACGGAAAATGCAGCAATTACATATGCCCCACTGTCCATAACTCTACAACCTGGCATTCTGACTATAAGGTCAAAGGGCTATGTGATTCTAACCTCATTTCCATGGACATCACCTTCTTCTCAGAGGACGGAGAGCTATCATCCCTGGGAAAGGAGGGCACAGGGTTCAGAAGTAACTACTTTGCTTATGAAACTGGAGGCAAGGCCTGCAAAATGCAATACTGCAAGCATTGGGGAGTCAGACTCCCATCAGGTGTCTGGTTCGAGATGGCTGATAAGGATCTCTTTGCTGCAGCCAGATTCCCTGAATGCCCAGAAGGGTCAAGTATCTCTGCTCCATCTCAGACCTCAGTGGATGTAAGTCTAATTCAGGACGTTGAGAGGATCTTGGATTATTCCCTCTGCCAAGAAACCTGGAGCAAAATCAGAGCGGGTCTTCCAATCTCTCCAGTGGATCTCAGCTATCTTGCTCCTAAAAACCCAGGAACCGGTCCTGCTTTCACCATAATCAATGGTACCCTAAAATACTTTGAGACCAGATACATCAGAGTCGATATTGCTGCTCCAATCCTCTCAAGAATGGTCGGAATGATCAGTGGAACTACCACAGAAAGGGAACTGTGGGATGACTGGGCACCATATGAAGACGTGGAAATTGGACCCAATGGAGTTCTGAGGACCAGTTCAGGATATAAGTTTCCTTTATACATGATTGGACATGGTATGTTGGACTCCGATCTTCATCTTAGCTCAAAGGCTCAGGTGTTCGAACATCCTCACATTCAAGACGCTGCTTCGCAACTTCCTGATGATGAGAGTTTATTTTTTGGTGATACTGGGCTATCCAAAAATCCAATCGAGCTTGTAGAAGGTTGGTTCAGTAGTTGGAAAAGCTCTATTGCCTCTTTTTTCTTTATCATAGGGTTAATCATTGGACTATTCTTGGTTCTCCGAGTTGGTATCCATCTTTGCATTAAATTAAAGCACACCAAGAAAAGACAGATTTATACAGACATAGAGATGAACCGACTTGGAAAGTAACTCAAATCCTGCTAGGTATGAAAAAAACTAACaGATATCACGCtcgagGCCACCATGAAGTGCCTGTTGTACTTAGCCTTCCTGTTCATCGGGGTGAATTGCCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAAGATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGGAGGGCTTTAACTGTTATTTCCCTCTGCAGAGCTACGGCTTCCAGCCAACAAACGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACCTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAAcggCTCTGGATCCGGCTACATCCCCGAGGCCCCCAGAGACGGCCAGGCCTACGTGCGGAAGGACGGCGAGTGGGTACTGctcagcaccttcctgggcAGCAGTTGGAAAAGCTCCATCGCCTCCTTTTTCTTTATCATCGGCCTGATCATCGGACTGTTCCTGGTGCTCCGCGTGGGTATCCACCTGTGCATCAAGCTGAAGCACACCAAGAAAAGACAGATTTATACAGACATCGAGATGAACCGACTTGGAAAGTAAGCTAGCCAGATTCTTCATGTTTGGACCAAATCAACTTGTGATACCATGCTCAAAGAGGCCTCAATTATATTTGAGTTTTTAATTTTTATGAAAAAAACTAACAGCAATCATGGAAGTCCACGATTTTGAGACCGACGAGTTCAATGATTTCAATGAAGATGACTATGCCACAAGAGAATTCCTGAATCCCGATGAGCGCATGACGTACTTGAATCATGCTGATTACAATTTGAATTCTCCTCTAATTAGTGATGATATTGACAATTTGATCAGGAAATTCAATTCTCTTCCGATTCCCTCGATGTGGGATAGTAAGAACTGGGATGGAGTTCTTGAGATGTTAACATCATGTCAAGCCAATCCCATCTCAACATCTCAGATGCATAAATGGATGGGAAGTTGGTTAATGTCTGATAATCATGATGCCAGTCAAGGGTATAGTTTTTTACATGAAGTGGACAAAGAGGCAGAAATAACATTTGACGTGGTGGAGACCTTCATCCGCGGCTGGGGCAACAAACCAATTGAATACATCAAAAAGGAAAGATGGACTGACTCATTCAAAATTCTCGCTTATTTGTGTCAAAAGTTTTTGGACTTACACAAGTTGACATTAATCTTAAATGCTGTCTCTGAGGTGGAATTGCTCAACTTGGCGAGGACTTTCAAAGGCAAAGTCAGAAGAAGTTCTCATGGAACGAACATATGCAGGATTAGGGTTCCCAGCTTGGGTCCTACTTTTATTTCAGAAGGATGGGCTTACTTCAAGAAACTTGATATTCTAATGGACCGAAACTTTCTGTTAATGGTCAAAGATGTGATTATAGGGAGGATGCAAACGGTGCTATCCATGGTATGTAGAATAGACAACCTGTTCTCAGAGCAAGACATCTTCTCCCTTCTAAATATCTACAGAATTGGAGATAAAATTGTGGAGAGGCAGGGAAATTTTTCTTATGACTTGATTAAAATGGTGGAACCGATATGCAACTTGAAGCTGATGAAATTAGCAAGAGAATCAAGGCCTTTAGTCCCACAATTCCCTCATTTTGAAAATCATATCAAGACTTCTGTTGATGAAGGGGCAAAAATTGACCGAGGTATAAGATTCCTCCATGATCAGATAATGAGTGTGAAAACAGTGGATCTCACACTGGTGATTTATGGATCGTTCAGACATTGGGGTCATCCTTTTATAGATTATTACACTGGACTAGAAAAATTACATTCCCAAGTAACCATGAAGAAAGATATTGATGTGTCATATGCAAAAGCACTTGCAAGTGATTTAGCTCGGATTGTTCTATTTCAACAGTTCAATGATCATAAAAAGTGGTTCGTGAATGGAGACTTGCTCCCTCATGATCATCCCTTTAAAAGTCATGTTAAAGAAAATACATGGCCCACAGCTGCTCAAGTTCAAGATTTTGGAGATAAATGGCATGAACTTCCGCTGATTAAATGTTTTGAAATACCCGACTTACTAGACCCATCGATAATATACTCTGACAAAAGTCATTCAATGAATAGGTCAGAGGTGTTGAAACATGTCCGAATGAATCCGAACACTCCTATCCCTAGTAAAAAGGTGTTGCAGACTATGTTGGACACAAAGGCTACCAATTGGAAAGAATTTCTTAAAGAGATTGATGAGAAGGGCTTAGATGATGATGATCTAATTATTGGTCTTAAAGGAAAGGAGAGGGAACTGAAGTTGGCAGGTAGATTTTTCTCCCTAATGTCTTGGAAATTGCGAGAATACTTTGTAATTACCGAATATTTGATAAAGACTCATTTCGTCCCTATGTTTAAAGGCCTGACAATGGCGGACGATCTAACTGCAGTCATTAAAAAGATGTTAGATTCCTCATCCGGCCAAGGATTGAAGTCATATGAGGCAATTTGCATAGCCAATCACATTGATTACGAAAAATGGAATAACCACCAAAGGAAGTTATCAAACGGCCCAGTGTTCCGAGTTATGGGCCAGTTCTTAGGTTATCCATCCTTAATCGAGAGAACTCATGAATTTTTTGAGAAAAGTCTTATATACTACAATGGAAGACCAGACTTGATGCGTGTTCACAACAACACACTGATCAATTCAACCTCCCAACGAGTTTGTTGGCAAGGACAAGAGGGTGGACTGGAAGGTCTACGGCAAAAAGGATGGACTATCCTCAATCTACTGGTTATTCAAAGAGAGGCTAAAATCAGAAACACTGCTGTCAAAGTCTTGGCACAAGGTGATAATCAAGTTATTTGCACACAGTATAAAACGAAGAAATCGAGAAACGTTGTAGAATTACAGGGTGCTCTCAATCAAATGGTTTCTAATAATGAGAAAATTATGACTGCAATCAAAATAGGGACAGGGAAGTTAGGACTTTTGATAAATGACGATGAGACTATGCAATCTGCAGATTACTTGAATTATGGAAAAATACCGATTTTCCGTGGAGTGATTAGAGGGTTAGAGACCAAGAGATGGTCACGAGTGACTTGTGTCACCAATGACCAAATACCCACTTGTGCTAATATAATGAGCTCAGTTTCCACAAATGCTCTCACCGTAGCTCATTTTGCTGAGAACCCAATCAATGCCATGATACAGTACAATTATTTTGGGACATTTGCTAGACTCTTGTTGATGATGCATGATCCTGCTCTTCGTCAATCATTGTATGAAGTTCAAGATAAGATACCGGGCTTGCACAGTTCTACTTTCAAATACGCCATGTTGTATTTGGACCCTTCCATTGGAGGAGTGTCGGGCATGTCTTTGTCCAGGTTTTTGATTAGAGCCTTCCCAGATCCCGTAACAGAAAGTCTCTCATTCTGGAGATTCATCCATGTACATGCTCGAAGTGAGCATCTGAAGGAGATGAGTGCAGTATTTGGAAACCCCGAGATAGCCAAGTTTCGAATAACTCACATAGACAAGCTAGTAGAAGATCCAACCTCTCTGAACATCGCTATGGGAATGAGTCCAGCGAACTTGTTAAAGACTGAGGTTAAAAAATGCTTAATCGAATCAAGACAAACCATCAGGAACCAGGTGATTAAGGATGCAACCATATATTTGTATCATGAAGAGGATCGGCTCAGAAGTTTCTTATGGTCAATAAATCCTCTGTTCCCTAGATTTTTAAGTGAATTCAAATCAGGCACTTTTTTGGGAGTCGCAGACGGGCTCATCAGTCTATTTCAAAATTCTCGTACTATTCGGAACTCCTTTAAGAAAAAGTATCATAGGGAATTGGATGATTTGATTGTGAGGAGTGAGGTATCCTCTTTGACACATTTAGGGAAACTTCATTTGAGAAGGGGATCATGTAAAATGTGGACATGTTCAGCTACTCATGCTGACACATTAAGATACAAATCCTGGGGCCGTACAGTTATTGGGACAACTGTACCCCATCCATTAGAAATGTTGGGTCCACAACATCGAAAAGAGACTCCTTGTGCACCATGTAACACATCAGGGTTCAATTATGTTTCTGTGCATTGTCCAGACGGGATCCATGACGTCTTTAGTTCACGGGGACCATTGCCTGCTTATCTAGGGTCTAAAACATCTGAATCTACATCTATTTTGCAGCCTTGGGAAAGGGAAAGCAAAGTCCCACTGATTAAAAGAGCTACACGTCTTAGAGATGCTATCTCTTGGTTTGTTGAACCCGACTCTAAACTAGCAATGACTATACTTTCTAACATCCACTCTTTAACAGGCGAAGAATGGACCAAAAGGCAGCATGGGTTCAAAAGAACAGGGTCTGCCCTTCATAGGTTTTCGACATCTCGGATGAGCCATGGTGGGTTCGCATCTCAGAGCACTGCAGCATTGACCAGGTTGATGGCAACTACAGACACCATGAGGGATCTGGGAGATCAGAATTTCGACTTTTTATTCCAAGCAACGTTGCTCTATGCTCAAATTACCACCACTGTTGCAAGAGACGGATGGATCACCAGTTGTACAGATCATTATCATATTGCCTGTAAGTCCTGTTTGAGACCCATAGAAGAGATCACCCTGGACTCAAGTATGGACTACACGCCCCCAGATGTATCCCATGTGCTGAAGACATGGAGGAATGGGGAAGGTTCGTGGGGACAAGAGATAAAACAGATCTATCCTTTAGAAGGGAATTGGAAGAATTTAGCACCTGCTGAGCAATCCTATCAAGTCGGCAGATGTATAGGTTTTCTATATGGAGACTTGGCGTATAGAAAATCTACTCATGCCGAGGACAGTTCTCTATTTCCTCTATCTATACAAGGTCGTATTAGAGGTCGAGGTTTCTTAAAAGGGTTGCTAGACGGATTAATGAGAGCAAGTTGCTGCCAAGTAATACACCGGAGAAGTCTGGCTCATTTGAAGAGGCCGGCCAACGCAGTGTACGGAGGTTTGATTTACTTGATTGATAAATTGAGTGTATCACCTCCATTCCTTTCTCTTACTAGATCAGGACCTATTAGAGACGAATTAGAAACGATTCCCCACAAGATCCCAACCTCCTATCCGACAAGCAACCGTGATATGGGGGTGATTGTCAGAAATTACTTCAAATACCAATGCCGTCTAATTGAAAAGGGAAAATACAGATCACATTATTCACAATTATGGTTATTCTCAGATGTCTTATCCATAGACTTCATTGGACCATTCTCTATTTCCACCACCCTCTTGCAAATCCTATACAAGCCATTTTTATCTGGGAAAGATAAGAATGAGTTGAGAGAGCTGGCAAATCTTTCTTCATTGCTAAGATCAGGAGAGGGGTGGGAAGACATACATGTGAAATTCTTCACCAAGGACATATTATTGTGTCCAGAGGAAATCAGACATGCTTGCAAGTTCGGGATTGCTAAGGATAATAATAAAGACATGAGCTATCCCCCTTGGGGAAGGGAATCCAGAGGGACAATTACAACAATCCCTGTTTATTATACGACCACCCCTTACCCAAAGATGCTAGAGATGCCTCCAAGAATCCAAAATCCCCTGCTGTCCGGAATCAGGTTGGGCCAATTACCAACTGGCGCTCATTATAAAATTCGGAGTATATTACATGGAATGGGAATCCATTACAGGGACTTCTTGAGTTGTGGAGACGGCTCCGGAGGGATGACTGCTGCATTACTACGAGAAAATGTGCATAGCAGAGGAATATTCAATAGTCTGTTAGAATTATCAGGGTCAGTCATGCGAGGCGCCTCTCCTGAGCCCCCCAGTGCCCTAGAAACTTTAGGAGGAGATAAATCGAGATGTGTAAATGGTGAAACATGTTGGGAATATCCATCTGACTTATGTGACCCAAGGACTTGGGACTATTTCCTCCGACTCAAAGCAGGCTTGGGGCTTCAAATTGATTTAATTGTAATGGATATGGAAGTTCGGGATTCTTCTACTAGCCTGAAAATTGAGACGAATGTTAGAAATTATGTGCACCGGATTTTGGATGAGCAAGGAGTTTTAATCTACAAGACTTATGGAACATATATTTGTGAGAGCGAAAAGAATGCAGTAACAATCCTTGGTCCCATGTTCAAGACGGTCGACTTAGTTCAAACAGAATTTAGTAGTTCTCAAACGTCTGAAGTATATATGGTATGTAAAGGTTTGAAGAAATTAATCGATGAACCCAATCCCGATTGGTCTTCCATCAATGAATCCTGGAAAAACCTGTACGCATTCCAGTCATCAGAACAGGAATTTGCCAGAGCAAAGAAGGTTAGTACATACTTTACCTTGACAGGTATTCCCTCCCAATTCATTCCTGATCCTTTTGTAAACATTGAGACTATGCTACAAATATTCGGAGTACCCACGGGTGTGTCTCATGCGGCTGCCTTAAAATCATCTGATAGACCTGCAGATTTATTGACCATTAGCCTTTTTTATATGGCGATTATATCGTATTATAACATCAATCATATCAGAGTAGGACCGATACCTCCGAACCCCCCATCAGATGGAATTGCACAAAATGTGGGGATCGCTATAACTGGTATAAGCTTTTGGCTGAGTTTGATGGAGAAAGACATTCCACTATATCAACAGTGTTTAGCAGTTATCCAGCAATCATTCCCGATTAGGTGGGAGGCTGTTTCAGTAAAAGGAGGATACAAGCAGAAGTGGAGTACTAGAGGTGATGGGCTCCCAAAAGATACCCGAACTTCAGACTCCTTGGCCCCAATCGGGAACTGGATCAGATCTCTGGAATTGGTCCGAAACCAAGTTCGTCTAAATCCATTCAATGAGATCTTGTTCAATCAGCTATGTCGTACAGTGGATAATCATTTGAAATGGTCAAATTTGCGAAGAAACACAGGAATGATTGAATGGATCAATAGACGAATTTCAAAAGAAGACCGGTCTATACTGATGTTGAAGAGTGACCTACACGAGGAAAACTCTTGGAGAGATTAAAAAATCATGAGGAGACTCCAAACTTTAAGTATGAAAAAAACTTTGATCCTTAAGACCCTCTTGTGGTTTTTATTTTTTATCTGGTTTTGTGGTCTTCGT。
在其他实施方式中，核酸包括SEQ ID NO：27的序列，如下所示(VSV载体：ConvacV5 South Africa)：
ACGAAGACAAACAAACCATTATTATCATTAAAAGGCTCAGGAGAAACTTTAACAGTAATCAAAATGTCTGTTACAGTCAAGAGAATCATTGACAACACAGTCATAGTTCCAAAACTTCCTGCAAATGAGGATCCAGTGGAATACCCGGCAGATTACTTCAGAAAATCAAAGGAGATTCCTCTTTACATCAATACTACAAAAAGTTTGTCAGATCTAAGAGGATATGTCTACCAAGGCCTCAAATCCGGAAATGTATCAATCATACATGTCAACAGCTACTTGTATGGAGCATTAAAGGACATCCGGGGTAAGTTGGATAAAGATTGGTCAAGTTTCGGAATAAACATCGGGAAAGCAGGGGATACAATCGGAATATTTGACCTTGTATCCTTGAAAGCCCTGGACGGCGTACTTCCAGATGGAGTATCGGATGCTTCCAGAACCAGCGCAGATGACAAATGGTTGCCTTTGTATCTACTTGGCTTATACAGAGTGGGCAGAACACAAATGCCTGAATACAGAAAAAAGCTCATGGATGGGCTGACAAATCAATGCAAAATGATCAATGAACAGTTTGAACCTCTTGTGCCAGAAGGTCGTGACATTTTTGATGTGTGGGGAAATGACAGTAATTACACAAAAATTGTCGCTGCAGTGGACATGTTCTTCCACATGTTCAAAAAACATGAATGTGCCTCGTTCAGATACGGAACTATTGTTTCCAGATTCAAAGATTGTGCTGCATTGGCAACATTTGGACACCTCTGCAAAATAACCGGAATGTCTACAGAAGATGTAACGACCTGGATCTTGAACCGAGAAGTTGCAGATGAAATGGTCCAAATGATGCTTCCAGGCCAAGAAATTGACAAGGCCGATTCATACATGCCTTATTTGATCGACTTTGGATTGTCTTCTAAGTCTCCATATTCTTCCGTCAAAAACCCTGCCTTCCACTTCTGGGGGCAATTGACAGCTCTTCTGCTCAGATCCACCAGAGCAAGGAATGCCCGACAGCCTGATGACATTGAGTATACATCTCTTACTACAGCAGGTTTGTTGTACGCTTATGCAGTAGGATCCTCTGCCGACTTGGCACAACAGTTTTGTGTTGGAGATAACAAATACACTCCAGATGATAGTACCGGAGGATTGACGACTAATGCACCGCCACAAGGCAGAGATGTGGTCGAATGGCTCGGATGGTTTGAAGATCAAAACAGAAAACCGACTCCTGATATGATGCAGTATGCGAAAAGAGCAGTCATGTCACTGCAAGGCCTAAGAGAGAAGACAATTGGCAAGTATGCTAAGTCAGAATTTGACAAATGACCCTATAATTCTCAGATCACCTATTATATATTATGCTACATATGAAAAAAACTAACAGATATCATGGATAATCTCACAAAAGTTCGTGAGTATCTCAAGTCCTATTCTCGTCTGGATCAGGCGGTAGGAGAGATAGATGAGATCGAAGCACAACGAGCTGAAAAGTCCAATTATGAGTTGTTCCAAGAGGATGGAGTGGAAGAGCATACTAAGCCCTCTTATTTTCAGGCAGCAGATGATTCTGACACAGAATCTGAACCAGAAATTGAAGACAATCAAGGTTTGTATGCACCAGATCCAGAAGCTGAGCAAGTTGAAGGCTTTATACAGGGGCCTTTAGATGACTATGCAGATGAGGAAGTGGATGTTGTATTTACTTCGGACTGGAAACAGCCTGAGCTTGAATCTGACGAGCATGGAAAGACCTTACGGTTGACATCGCCAGAGGGTTTAAGTGGAGAGCAGAAATCCCAGTGGCTTTCGACGATTAAAGCAGTCGTGCAAAGTGCCAAATACTGGAATCTGGCAGAGTGCACATTTGAAGCATCGGGAGAAGGGGTCATTATGAAGGAGCGCCAGATAACTCCGGATGTATATAAGGTCACTCCAGTGATGAACACACATCCGTCCCAATCAGAAGCAGTATCAGATGTTTGGTCTCTCTCAAAGACATCCATGACTTTCCAACCCAAGAAAGCAAGTCTTCAGCCTCTCACCATATCCTTGGATGAATTGTTCTCATCTAGAGGAGAGTTCATCTCTGTCGGAGGTGACGGACGAATGTCTCATAAAGAGGCCATCCTGCTCGGCCTGAGATACAAAAAGTTGTACAATCAGGCGAGAGTCAAATATTCTCTGTAGACTATGAAAAAAAGTAACAGATATCACGATCTAAGTGTTATCCCAATCCATTCATCATGAGTTCCTTAAAGAAGATTCTCGGTCTGAAGGGGAAAGGTAAGAAATCTAAGAAATTAGGGATCGCACCACCCCCTTATGAAGAGGACACTAGCATGGAGTATGCTCCGAGCGCTCCAATTGACAAATCCTATTTTGGAGTTGACGAGATGGACACCTATGATCCGAATCAATTAAGATATGAGAAATTCTTCTTTACAGTGAAAATGACGGTTAGATCTAATCGTCCGTTCAGAACATACTCAGATGTGGCAGCCGCTGTATCCCATTGGGATCACATGTACATCGGAATGGCAGGGAAACGTCCCTTCTACAAAATCTTGGCTTTTTTGGGTTCTTCTAATCTAAAGGCCACTCCAGCGGTATTGGCAGATCAAGGTCAACCAGAGTATCACACTCACTGCGAAGGCAGGGCTTATTTGCCACATAGGATGGGGAAGACCCCTCCCATGCTCAATGTACCAGAGCACTTCAGAAGACCATTCAATATAGGTCTTTACAAGGGAACGATTGAGCTCACAATGACCATCTACGATGATGAGTCACTGGAAGCAGCTCCTATGATCTGGGATCATTTCAATTCTTCCAAATTTTCTGATTTCAGAGAGAAGGCCTTAATGTTTGGCCTGATTGTCGAGAAAAAGGCATCTGGAGCGTGGGTCCTGGATTCTATCAGCCACTTCAAATGAGCTAGTCTAACTTCTAGCTTCTGAACAATCCCCGGTTTACTCAGTCTCTCCTAATTCCAGCCTCTCGAACAACTAATATCCTGTCTTTTCTATCCCTATGAAAAAAACTAACAGAGATCGATCTGTTTACGCGTCACTATGAAGTGCCTTTTGTACTTAGCCTTTTTATTCATTGGGGTGAATTGCAAGTTCACCATAGTTTTTCCACACAACCAAAAAGGAAACTGGAAAAATGTTCCTTCTAATTACCATTATTGCCCGTCAAGCTCAGATTTAAATTGGCATAATGACTTAATAGGCACAGCCATACAAGTCAAAATGCCCAAGAGTCACAAGGCTATTCAAGCAGACGGTTGGATGTGTCATGCTTCCAAATGGGTCACTACTTGTGATTTCCGCTGGTATGGACCGAAGTATATAACACAGTCCATCCGATCCTTCACTCCATCTGTAGAACAATGCAAGGAAAGCATTGAACAAACGAAACAAGGAACTTGGCTGAATCCAGGCTTCCCTCCTCAAAGTTGTGGATATGCAACTGTGACGGATGCCGAAGCAGTGATTGTCCAGGTGACTCCTCACCATGTGCTGGTTGATGAATACACAGGAGAATGGGTTGATTCACAGTTCATCAACGGAAAATGCAGCAATTACATATGCCCCACTGTCCATAACTCTACAACCTGGCATTCTGACTATAAGGTCAAAGGGCTATGTGATTCTAACCTCATTTCCATGGACATCACCTTCTTCTCAGAGGACGGAGAGCTATCATCCCTGGGAAAGGAGGGCACAGGGTTCAGAAGTAACTACTTTGCTTATGAAACTGGAGGCAAGGCCTGCAAAATGCAATACTGCAAGCATTGGGGAGTCAGACTCCCATCAGGTGTCTGGTTCGAGATGGCTGATAAGGATCTCTTTGCTGCAGCCAGATTCCCTGAATGCCCAGAAGGGTCAAGTATCTCTGCTCCATCTCAGACCTCAGTGGATGTAAGTCTAATTCAGGACGTTGAGAGGATCTTGGATTATTCCCTCTGCCAAGAAACCTGGAGCAAAATCAGAGCGGGTCTTCCAATCTCTCCAGTGGATCTCAGCTATCTTGCTCCTAAAAACCCAGGAACCGGTCCTGCTTTCACCATAATCAATGGTACCCTAAAATACTTTGAGACCAGATACATCAGAGTCGATATTGCTGCTCCAATCCTCTCAAGAATGGTCGGAATGATCAGTGGAACTACCACAGAAAGGGAACTGTGGGATGACTGGGCACCATATGAAGACGTGGAAATTGGACCCAATGGAGTTCTGAGGACCAGTTCAGGATATAAGTTTCCTTTATACATGATTGGACATGGTATGTTGGACTCCGATCTTCATCTTAGCTCAAAGGCTCAGGTGTTCGAACATCCTCACATTCAAGACGCTGCTTCGCAACTTCCTGATGATGAGAGTTTATTTTTTGGTGATACTGGGCTATCCAAAAATCCAATCGAGCTTGTAGAAGGTTGGTTCAGTAGTTGGAAAAGCTCTATTGCCTCTTTTTTCTTTATCATAGGGTTAATCATTGGACTATTCTTGGTTCTCCGAGTTGGTATCCATCTTTGCATTAAATTAAAGCACACCAAGAAAAGACAGATTTATACAGACATAGAGATGAACCGACTTGGAAAGTAACTCAAATCCTGCTAGGTATGAAAAAAACTAACaGATATCACGCtcgagGCCACCATGAAGTGCCTGTTGTACTTAGCCTTCCTGTTCATCGGGGTGAATTGCCGCTTTCCCAATATCACAAACCTGTGCCCTTTTGGCGAGGTGTTCAACGCAACCCGCTTCGCCAGCGTGTACGCCTGGAATAGGAAGCGCATCTCCAACTGCGTGGCCGACTATTCTGTGCTGTACAACAGCGCCTCCTTCTCTACCTTTAAGTGCTATGGCGTGAGCCCCACAAAGCTGAATGACCTGTGCTTTACCAACGTGTACGCCGATTCCTTCGTGATCAGGGGCGACGAGGTGCGCCAGATCGCACCAGGACAGACAGGCAATATCGCAGACTACAATTATAAGCTGCCTGACGATTTCACCGGCTGCGTGATCGCCTGGAACTCTAACAATCTGGATAGCAAAGTGGGCGGCAACTACAATTATCTGTACCGGCTGTTTAGAAAGTCTAATCTGAAGCCATTCGAGAGGGACATCTCCACAGAAATCTACCAGGCCGGCTCTACCCCCTGCAATGGCGTGAAGGGCTTTAACTGTTATTTCCCTCTGCAGAGCTACGGCTTCCAGCCAACATATGGCGTGGGCTATCAGCCCTACCGCGTGGTGGTGCTGTCTTTTGAGCTGCTGCACGCACCTGCAACAGTGTGCGGACCAAAGAAGAGCACCAATCTGGTGAAGAACAAGTGCGTGAACTTCAAcggCTCTGGATCCGGCTACATCCCCGAGGCCCCCAGAGACGGCCAGGCCTACGTGCGGAAGGACGGCGAGTGGGTACTGctcagcaccttcctgggcAGCAGTTGGAAAAGCTCCATCGCCTCCTTTTTCTTTATCATCGGCCTGATCATCGGACTGTTCCTGGTGCTCCGCGTGGGTATCCACCTGTGCATCAAGCTGAAGCACACCAAGAAAAGACAGATTTATACAGACATCGAGATGAACCGACTTGGAAAGTAAGCTAGCCAGATTCTTCATGTTTGGACCAAATCAACTTGTGATACCATGCTCAAAGAGGCCTCAATTATATTTGAGTTTTTAATTTTTATGAAAAAAACTAACAGCAATCATGGAAGTCCACGATTTTGAGACCGACGAGTTCAATGATTTCAATGAAGATGACTATGCCACAAGAGAATTCCTGAATCCCGATGAGCGCATGACGTACTTGAATCATGCTGATTACAATTTGAATTCTCCTCTAATTAGTGATGATATTGACAATTTGATCAGGAAATTCAATTCTCTTCCGATTCCCTCGATGTGGGATAGTAAGAACTGGGATGGAGTTCTTGAGATGTTAACATCATGTCAAGCCAATCCCATCTCAACATCTCAGATGCATAAATGGATGGGAAGTTGGTTAATGTCTGATAATCATGATGCCAGTCAAGGGTATAGTTTTTTACATGAAGTGGACAAAGAGGCAGAAATAACATTTGACGTGGTGGAGACCTTCATCCGCGGCTGGGGCAACAAACCAATTGAATACATCAAAAAGGAAAGATGGACTGACTCATTCAAAATTCTCGCTTATTTGTGTCAAAAGTTTTTGGACTTACACAAGTTGACATTAATCTTAAATGCTGTCTCTGAGGTGGAATTGCTCAACTTGGCGAGGACTTTCAAAGGCAAAGTCAGAAGAAGTTCTCATGGAACGAACATATGCAGGATTAGGGTTCCCAGCTTGGGTCCTACTTTTATTTCAGAAGGATGGGCTTACTTCAAGAAACTTGATATTCTAATGGACCGAAACTTTCTGTTAATGGTCAAAGATGTGATTATAGGGAGGATGCAAACGGTGCTATCCATGGTATGTAGAATAGACAACCTGTTCTCAGAGCAAGACATCTTCTCCCTTCTAAATATCTACAGAATTGGAGATAAAATTGTGGAGAGGCAGGGAAATTTTTCTTATGACTTGATTAAAATGGTGGAACCGATATGCAACTTGAAGCTGATGAAATTAGCAAGAGAATCAAGGCCTTTAGTCCCACAATTCCCTCATTTTGAAAATCATATCAAGACTTCTGTTGATGAAGGGGCAAAAATTGACCGAGGTATAAGATTCCTCCATGATCAGATAATGAGTGTGAAAACAGTGGATCTCACACTGGTGATTTATGGATCGTTCAGACATTGGGGTCATCCTTTTATAGATTATTACACTGGACTAGAAAAATTACATTCCCAAGTAACCATGAAGAAAGATATTGATGTGTCATATGCAAAAGCACTTGCAAGTGATTTAGCTCGGATTGTTCTATTTCAACAGTTCAATGATCATAAAAAGTGGTTCGTGAATGGAGACTTGCTCCCTCATGATCATCCCTTTAAAAGTCATGTTAAAGAAAATACATGGCCCACAGCTGCTCAAGTTCAAGATTTTGGAGATAAATGGCATGAACTTCCGCTGATTAAATGTTTTGAAATACCCGACTTACTAGACCCATCGATAATATACTCTGACAAAAGTCATTCAATGAATAGGTCAGAGGTGTTGAAACATGTCCGAATGAATCCGAACACTCCTATCCCTAGTAAAAAGGTGTTGCAGACTATGTTGGACACAAAGGCTACCAATTGGAAAGAATTTCTTAAAGAGATTGATGAGAAGGGCTTAGATGATGATGATCTAATTATTGGTCTTAAAGGAAAGGAGAGGGAACTGAAGTTGGCAGGTAGATTTTTCTCCCTAATGTCTTGGAAATTGCGAGAATACTTTGTAATTACCGAATATTTGATAAAGACTCATTTCGTCCCTATGTTTAAAGGCCTGACAATGGCGGACGATCTAACTGCAGTCATTAAAAAGATGTTAGATTCCTCATCCGGCCAAGGATTGAAGTCATATGAGGCAATTTGCATAGCCAATCACATTGATTACGAAAAATGGAATAACCACCAAAGGAAGTTATCAAACGGCCCAGTGTTCCGAGTTATGGGCCAGTTCTTAGGTTATCCATCCTTAATCGAGAGAACTCATGAATTTTTTGAGAAAAGTCTTATATACTACAATGGAAGACCAGACTTGATGCGTGTTCACAACAACACACTGATCAATTCAACCTCCCAACGAGTTTGTTGGCAAGGACAAGAGGGTGGACTGGAAGGTCTACGGCAAAAAGGATGGACTATCCTCAATCTACTGGTTATTCAAAGAGAGGCTAAAATCAGAAACACTGCTGTCAAAGTCTTGGCACAAGGTGATAATCAAGTTATTTGCACACAGTATAAAACGAAGAAATCGAGAAACGTTGTAGAATTACAGGGTGCTCTCAATCAAATGGTTTCTAATAATGAGAAAATTATGACTGCAATCAAAATAGGGACAGGGAAGTTAGGACTTTTGATAAATGACGATGAGACTATGCAATCTGCAGATTACTTGAATTATGGAAAAATACCGATTTTCCGTGGAGTGATTAGAGGGTTAGAGACCAAGAGATGGTCACGAGTGACTTGTGTCACCAATGACCAAATACCCACTTGTGCTAATATAATGAGCTCAGTTTCCACAAATGCTCTCACCGTAGCTCATTTTGCTGAGAACCCAATCAATGCCATGATACAGTACAATTATTTTGGGACATTTGCTAGACTCTTGTTGATGATGCATGATCCTGCTCTTCGTCAATCATTGTATGAAGTTCAAGATAAGATACCGGGCTTGCACAGTTCTACTTTCAAATACGCCATGTTGTATTTGGACCCTTCCATTGGAGGAGTGTCGGGCATGTCTTTGTCCAGGTTTTTGATTAGAGCCTTCCCAGATCCCGTAACAGAAAGTCTCTCATTCTGGAGATTCATCCATGTACATGCTCGAAGTGAGCATCTGAAGGAGATGAGTGCAGTATTTGGAAACCCCGAGATAGCCAAGTTTCGAATAACTCACATAGACAAGCTAGTAGAAGATCCAACCTCTCTGAACATCGCTATGGGAATGAGTCCAGCGAACTTGTTAAAGACTGAGGTTAAAAAATGCTTAATCGAATCAAGACAAACCATCAGGAACCAGGTGATTAAGGATGCAACCATATATTTGTATCATGAAGAGGATCGGCTCAGAAGTTTCTTATGGTCAATAAATCCTCTGTTCCCTAGATTTTTAAGTGAATTCAAATCAGGCACTTTTTTGGGAGTCGCAGACGGGCTCATCAGTCTATTTCAAAATTCTCGTACTATTCGGAACTCCTTTAAGAAAAAGTATCATAGGGAATTGGATGATTTGATTGTGAGGAGTGAGGTATCCTCTTTGACACATTTAGGGAAACTTCATTTGAGAAGGGGATCATGTAAAATGTGGACATGTTCAGCTACTCATGCTGACACATTAAGATACAAATCCTGGGGCCGTACAGTTATTGGGACAACTGTACCCCATCCATTAGAAATGTTGGGTCCACAACATCGAAAAGAGACTCCTTGTGCACCATGTAACACATCAGGGTTCAATTATGTTTCTGTGCATTGTCCAGACGGGATCCATGACGTCTTTAGTTCACGGGGACCATTGCCTGCTTATCTAGGGTCTAAAACATCTGAATCTACATCTATTTTGCAGCCTTGGGAAAGGGAAAGCAAAGTCCCACTGATTAAAAGAGCTACACGTCTTAGAGATGCTATCTCTTGGTTTGTTGAACCCGACTCTAAACTAGCAATGACTATACTTTCTAACATCCACTCTTTAACAGGCGAAGAATGGACCAAAAGGCAGCATGGGTTCAAAAGAACAGGGTCTGCCCTTCATAGGTTTTCGACATCTCGGATGAGCCATGGTGGGTTCGCATCTCAGAGCACTGCAGCATTGACCAGGTTGATGGCAACTACAGACACCATGAGGGATCTGGGAGATCAGAATTTCGACTTTTTATTCCAAGCAACGTTGCTCTATGCTCAAATTACCACCACTGTTGCAAGAGACGGATGGATCACCAGTTGTACAGATCATTATCATATTGCCTGTAAGTCCTGTTTGAGACCCATAGAAGAGATCACCCTGGACTCAAGTATGGACTACACGCCCCCAGATGTATCCCATGTGCTGAAGACATGGAGGAATGGGGAAGGTTCGTGGGGACAAGAGATAAAACAGATCTATCCTTTAGAAGGGAATTGGAAGAATTTAGCACCTGCTGAGCAATCCTATCAAGTCGGCAGATGTATAGGTTTTCTATATGGAGACTTGGCGTATAGAAAATCTACTCATGCCGAGGACAGTTCTCTATTTCCTCTATCTATACAAGGTCGTATTAGAGGTCGAGGTTTCTTAAAAGGGTTGCTAGACGGATTAATGAGAGCAAGTTGCTGCCAAGTAATACACCGGAGAAGTCTGGCTCATTTGAAGAGGCCGGCCAACGCAGTGTACGGAGGTTTGATTTACTTGATTGATAAATTGAGTGTATCACCTCCATTCCTTTCTCTTACTAGATCAGGACCTATTAGAGACGAATTAGAAACGATTCCCCACAAGATCCCAACCTCCTATCCGACAAGCAACCGTGATATGGGGGTGATTGTCAGAAATTACTTCAAATACCAATGCCGTCTAATTGAAAAGGGAAAATACAGATCACATTATTCACAATTATGGTTATTCTCAGATGTCTTATCCATAGACTTCATTGGACCATTCTCTATTTCCACCACCCTCTTGCAAATCCTATACAAGCCATTTTTATCTGGGAAAGATAAGAATGAGTTGAGAGAGCTGGCAAATCTTTCTTCATTGCTAAGATCAGGAGAGGGGTGGGAAGACATACATGTGAAATTCTTCACCAAGGACATATTATTGTGTCCAGAGGAAATCAGACATGCTTGCAAGTTCGGGATTGCTAAGGATAATAATAAAGACATGAGCTATCCCCCTTGGGGAAGGGAATCCAGAGGGACAATTACAACAATCCCTGTTTATTATACGACCACCCCTTACCCAAAGATGCTAGAGATGCCTCCAAGAATCCAAAATCCCCTGCTGTCCGGAATCAGGTTGGGCCAATTACCAACTGGCGCTCATTATAAAATTCGGAGTATATTACATGGAATGGGAATCCATTACAGGGACTTCTTGAGTTGTGGAGACGGCTCCGGAGGGATGACTGCTGCATTACTACGAGAAAATGTGCATAGCAGAGGAATATTCAATAGTCTGTTAGAATTATCAGGGTCAGTCATGCGAGGCGCCTCTCCTGAGCCCCCCAGTGCCCTAGAAACTTTAGGAGGAGATAAATCGAGATGTGTAAATGGTGAAACATGTTGGGAATATCCATCTGACTTATGTGACCCAAGGACTTGGGACTATTTCCTCCGACTCAAAGCAGGCTTGGGGCTTCAAATTGATTTAATTGTAATGGATATGGAAGTTCGGGATTCTTCTACTAGCCTGAAAATTGAGACGAATGTTAGAAATTATGTGCACCGGATTTTGGATGAGCAAGGAGTTTTAATCTACAAGACTTATGGAACATATATTTGTGAGAGCGAAAAGAATGCAGTAACAATCCTTGGTCCCATGTTCAAGACGGTCGACTTAGTTCAAACAGAATTTAGTAGTTCTCAAACGTCTGAAGTATATATGGTATGTAAAGGTTTGAAGAAATTAATCGATGAACCCAATCCCGATTGGTCTTCCATCAATGAATCCTGGAAAAACCTGTACGCATTCCAGTCATCAGAACAGGAATTTGCCAGAGCAAAGAAGGTTAGTACATACTTTACCTTGACAGGTATTCCCTCCCAATTCATTCCTGATCCTTTTGTAAACATTGAGACTATGCTACAAATATTCGGAGTACCCACGGGTGTGTCTCATGCGGCTGCCTTAAAATCATCTGATAGACCTGCAGATTTATTGACCATTAGCCTTTTTTATATGGCGATTATATCGTATTATAACATCAATCATATCAGAGTAGGACCGATACCTCCGAACCCCCCATCAGATGGAATTGCACAAAATGTGGGGATCGCTATAACTGGTATAAGCTTTTGGCTGAGTTTGATGGAGAAAGACATTCCACTATATCAACAGTGTTTAGCAGTTATCCAGCAATCATTCCCGATTAGGTGGGAGGCTGTTTCAGTAAAAGGAGGATACAAGCAGAAGTGGAGTACTAGAGGTGATGGGCTCCCAAAAGATACCCGAACTTCAGACTCCTTGGCCCCAATCGGGAACTGGATCAGATCTCTGGAATTGGTCCGAAACCAAGTTCGTCTAAATCCATTCAATGAGATCTTGTTCAATCAGCTATGTCGTACAGTGGATAATCATTTGAAATGGTCAAATTTGCGAAGAAACACAGGAATGATTGAATGGATCAATAGACGAATTTCAAAAGAAGACCGGTCTATACTGATGTTGAAGAGTGACCTACACGAGGAAAACTCTTGGAGAGATTAAAAAATCATGAGGAGACTCCAAACTTTAAGTATGAAAAAAACTTTGATCCTTAAGACCCTCTTGTGGTTTTTATTTTTTATCTGGTTTTGTGGTCTTCGT。
在另一方面中，本发明提供了一种编码重组融合蛋白的分离核酸，该重组融合蛋白包括(a)病毒的糖蛋白(G)或其部分，和(b)SARS-CoV-2刺突蛋白(S)或其部分。在一些实施方式中，病毒是弹状病毒。在一些实施方式中，病毒是狂犬病病毒或水疱性口炎病毒(VSV)。在具体实施方式中，病毒是狂犬病病毒。
在一个实施方式中，SARS-CoV-2刺突蛋白(S)的部分是SARS-CoV-2刺突蛋白(S)的受体结合位点。在另一个实施方式中，SARS-CoV-2刺突蛋白(S)的部分是S1结构域。在另一个实施方式中，SARS-CoV-2刺突蛋白(S)的部分是SARS-CoV-2刺突蛋白(S)的N-末端750个氨基酸。
在一些实施方式中，糖蛋白(G)包括在第333位用谷氨酸取代精氨酸的突变。在一些实施方式中，糖蛋白(G)的部分包括胞外结构域、胞质结构域和跨膜结构域。在一些实施方式中，糖蛋白(G)的部分包括胞外结构域的31个氨基酸。在一些实施方式中，糖蛋白(G)包括胞外结构域的31个氨基酸和全长胞质结构域。
在一些实施方式中，编码SARS-CoV-2刺突蛋白(S)或其部分的序列被密码子优化以在人类细胞中表达。在一些实施方式中，编码与糖蛋白(G)或其部分融合的SARS-CoV-2刺突蛋白(S)或其部分的序列被密码子优化以在人类细胞中表达。
在一些实施方式中，重组融合蛋白包括与狂犬病糖蛋白(G)或其部分融合的SARS-CoV-2刺突蛋白(S)的S1结构域的序列，在图18中显示为“BNSP333-COVID19-S1-RVG”中的元件“WuS1-RABVG”。
在一些实施方式中，重组融合蛋白包括与VSV糖蛋白(G)或其部分融合的SARS-CoV-2刺突蛋白(S)的S1结构域的序列，在图17中显示为“VSV-COVID19-S1-VSVG”中的元件“WuhanS-coVSV-G-tail”。
在其他方面，本发明提供具有双链、单链和/或双链和单链区域的组合以及本公开内容的任何序列的全部或部分互补物的核酸分子。
在一些方面中，本公开内容提供下述序列或其互补物：
Figure GDA0004183787440001861
Figure GDA0004183787440001871
Figure GDA0004183787440001881
药物组合物和制剂
本发明的疫苗可以配制成药物组合物。在一些实施方式中，疫苗含有活病毒。在一些实施方式中，疫苗含有灭活的病毒颗粒。在一些实施方式中，病毒是由本文所述的任何一种核酸构建体编码的重组病毒。
这样的药物组合物可以是适合施用于受试者(即哺乳动物)的形式，或者药物组合物可以进一步包括一种或多种药学上可接受的载体、一种或多种附加成分或这些的一些组合。如本领域公知的，药物组合物的各种组分可以生理学上可接受的盐的形式存在，比如与生理学上可接受的阳离子或阴离子组合。
在一个实施方式中，可用于实施本发明方法的药物组合物可包括佐剂。合适佐剂的非限制性实例是弗氏完全佐剂、弗氏不完全佐剂、Quil A、Detox、ISCOM或角鲨烯。在一个实施方式中，佐剂是ADVAXTM佐剂。在另一个实施方式中，佐剂包括Sepivac SWETM佐剂。在另一个实施方式中，佐剂是角鲨烯中的单磷酰脂A(MPLA)
Figure GDA0004183787440001882
在一个实施方式中，佐剂是在2％角鲨烯中的MPLA 3D(6-酰基)
Figure GDA0004183787440001883
在一个实施方式中，佐剂增加或诱导Th-1型免疫反应。药物组合物或疫苗组合物可包括本文所述的任何一种或多种佐剂。
在一些实施方式中，佐剂包括菊粉，例如，δ菊粉(例如，根据当前良好生产规范(Good Manufacturing Practices)制造的δ菊粉在本领域中称为Advax)。
在一个实施方式中，佐剂包含与TLR9激动剂(例如，CpG寡脱氧核苷酸(CpG ODN))一起配制的δ菊粉多糖。在一些实施方式中，佐剂是Advax-SMTM(Vaxine Pty Ltd，BedfordPark，Australia)。
在一些实施方式中，佐剂包括油组分和表面活性剂组分的混合物。
在一个实施方式中，组合物可以是油/表面活性剂分散体或油/表面活性剂溶液。
在其他实施方式中，佐剂包括乳剂，例如水包油(o/w)乳剂。
在一个实施方式中，组合物包括由一种或多种油形成的油组分。
在另一个实施方式中，组合物中的油和表面活性剂是可代谢的(生物可降解的)和生物相容的。
在一些实施方式中，组合物还包含除油和表面活性剂组分之外的组分。
在其他实施方式中，油组分和表面活性剂组分的比例可以变化。在一些实施方式中，当与一定体积的水性材料(例如水)混合时，水包油乳液包括油滴(例如，通过例如动态光散射(DLS)测定的亚微米油滴)。在其他实施方式中，油滴在与一定体积的水性材料(例如，水)混合时具有小于约300nm，例如约50至约290nm、约60至约280nm、约70至约270nm、约80至约260nm、约90至约250nm、约100至约240nm、约110至约230nm、约120至约220nm、约130至约210nm和约140至约200nm的平均直径。在一些实施方式中，油滴具有约160nm(例如，160±10nm)、约155nm(例如，155±10nm)、约142nm(例如，142±5nm)、约120nm(例如，120±40nm)或约100nm(例如，10±20nm)的平均直径。在一些实施方式中，油滴具有约142nm(例如，142±5nm)的平均直径。
在一个实施方式中，按体积计，总的油组分为佐剂组合物的约50％至约90％。在另一个实施方式中，按体积计，总的油组分不大于佐剂组合物的约50％、不大于约55％、不大于约60％、不大于约65％、不大于约70％、不大于约75％、不大于约80％、不大于约85％或不大于约90％。
在一些实施方式中，油包含萜类化合物(例如，支链的、不饱和的萜类化合物)。
在一个实施方式中，油包括角鲨烯。
在另一个实施方式中，油包括角鲨烯的饱和类似物。在一个实施方式中，角鲨烯的饱和类似物是角鲨烷。
在一个实施方式中，佐剂包括水乳液中的角鲨烯油。在另一个实施方式中，水乳液中的角鲨烯包括一种或多种非离子表面活性剂和/或其他油和/或稳定剂。
在其他实施方式中，佐剂包括约7mg至约13mg、约8mg至约12mg、约8mg至约11mg、约9mg至约10mg、约9.5mg至约9.75mg的角鲨烯(例如，每0.25ml或0.5ml疫苗剂量中角鲨烯的毫克数)。在一个实施方式中，佐剂包括约8.6mg、9.75mg、10.75mg或12.5mg的角鲨烯。
在另一个实施方式中，佐剂是水乳液中的角鲨烯，其包括约7至约13mg的角鲨烯(例如，每0.25ml或0.5ml疫苗剂量中角鲨烯的毫克数)，示例性地，约7至约13mg、约8至约12mg、约8至约11mg、约9至约10mg、约9.5至约9.75mg的角鲨烯(例如，每0.25ml或0.5ml疫苗剂量中的毫克数)。在一个实施方式中，佐剂是水乳液中的角鲨烯，其包括约9.75mg的角鲨烯(例如，每0.25ml或0.5ml疫苗剂量中角鲨烯的毫克数)。
在其他实施方式中，油组分包括一种或多种生育酚(例如，α、β、γ、δ、ε、ξ生育酚)。在一个实施方式中，生育酚是D-α-生育酚和/或DL-α-生育酚。在另一个实施方式中，α-生育酚是DL-α-生育酚。在其他实施方式中，佐剂中的油组分为包括角鲨烯和生育酚(例如DL-α-生育酚)的油组合。
在一些实施方式中，表面活性剂组分包括离子、非离子或两性离子表面活性剂，及其任何组合。在一个实施方式中，表面活性剂组分仅包括非离子表面活性剂(一种或多种)。
表面活性剂的实例包括但不限于聚氧乙烯脱水山梨糖醇酯表面活性剂(例如，Tweens、聚山梨醇酯，诸如例如聚山梨醇酯80(例如，TweenTM 80)，环氧乙烷、环氧丙烷、环氧丁烷的共聚物)、脱水山梨糖醇酯(例如，山梨糖醇三油酸酯(例如，SpanTM 85)、山梨糖醇单月桂酸酯)和聚氧乙烯月桂基醚(例如，Emulgen 104P)、辛苯聚醇(octoxynol)(例如，Triton X-100、IGEPAL CA-630/NP-40)、磷脂(例如，卵磷脂)和Brij表面活性剂(例如，聚氧乙烯4月桂基醚(Brij 30))。
在一些实施方式中，组合物中的表面活性剂(按油/表面活性剂组合物的体积％计)不超过约：49％、48％、47％、46％、45％、44％、43％、42％、41％、40％、39％、38％、37％、36％、35％、34％、33％、32％、31％、30％、29％、28％、27％、26％、25％、24％、23％、22％、21％、20％、19％、18％、17％、16％、15％、14％、13％、12％、11％或10％。
在一个实施方式中，表面活性剂组分由聚山梨醇酯80(例如，TweenTM 80)和山梨糖醇三油酸酯(例如，SpanTM 85)的混合物组成。在另一个实施方式中，表面活性剂组分由聚山梨醇酯80(例如，TweenTM 80)和山梨糖醇三油酸酯(例如，SpanTM 85)的混合物组成，体积比为山梨糖醇三油酸酯(例如，SpanTM 85)多于聚山梨醇酯80(例如，TweenTM 80)。在一些实施方式中，表面活性剂组分由以达到HLB约为8的体积比的聚山梨醇酯80(例如，TweenTM 80)和山梨糖醇三油酸酯(例如，SpanTM 85)的混合物组成。在一些实施方式中，表面活性剂组分由约1.175mg聚山梨醇酯80(例如，TweenTM 80)和约1.175mg山梨糖醇三油酸酯(例如，SpanTM85)的混合物(例如，每0.25ml或0.5ml疫苗剂量中角鲨烯的毫克数)组成。
在其他实施方式中，水性组分(例如，水)进一步包括一种或多种组分，例如溶质/盐/缓冲液。在一个实施方式中，盐(例如，钠盐)形成pH缓冲液(例如柠檬酸盐，磷酸盐)。在另一个实施方式中，一种或多种缓冲液包括但不限于柠檬酸盐缓冲液、磷酸盐缓冲液(例如，磷酸盐缓冲盐水、磷酸铵)、Tris缓冲液、硼酸盐缓冲液、琥珀酸盐缓冲液或组氨酸缓冲液。在一些实施方式中，缓冲的水性组分包括约1至约20mM的总缓冲液。
在其他实施方式中，水性组分的pH被缓冲在约pH 5.5至约pH 8.0，示例性地，约pH6.1至约pH 7.9、约pH 6.2至约pH 7.8、约pH 6.3至约pH 7.7、约pH 6.4至约pH 7.6、约pH6.5至约pH 7.5、约pH 6.6至约pH 7.4、约pH 6.7至约pH 7.3、约pH 6.8至约pH 7.2和约pH6.9至约pH 7.1。在一个实施方式中，水性组分的pH被缓冲在约pH：5.7、6.0、6.5、6.8或7.2。在另一个实施方式中，水性组分的pH被缓冲在约pH 6.0至约pH 6.5。在其他实施方式中，缓冲液是pH约6.5的10mM柠檬酸盐缓冲液。
在其他实施方式中，佐剂是水乳液中的角鲨烯，其包括(例如，每0.25ml或0.5ml疫苗剂量中角鲨烯的毫克数)约9.75mg角鲨烯、1.175mg聚山梨醇酯80(例如，TweenTM 80)、1.175mg山梨糖醇三油酸酯(例如，SpanTM 85)和pH约6.5的柠檬酸盐缓冲液。
在一些实施方式中，本公开内容的佐剂与疫苗共同配制或共同施用。
在其他实施方式中，本公开内容的佐剂不与疫苗共同配制或共同施用。
可用于本发明方法的药物组合物可适当地开发用于吸入、口服、直肠、阴道、肠胃外、局部、透皮、肺、鼻内、口腔、眼、鞘内、静脉内或其他施用途径。其他考虑的制剂包括规划的纳米颗粒、脂质体制剂、含有活性成分的重新密封的红细胞和基于免疫学的制剂。施用途径(一种或多种)对本领域技术人员来说是显而易见的并且取决于许多因素，包括所治疗疾病的类型和严重程度、所治疗的兽医或人类患者的类型和年龄等。
尽管本文提供的药物组合物的描述主要针对适合于合乎道德地施用于人类的药物组合物，但本领域技术人员应理解，这种组合物通常适合施用于各种动物。对适用于施用于人类的药物组合物的修改以使该组合物适用于各种动物的施用是众所周知的，并且普通的兽医药理学家可以仅通过普通的实验(如果有的话)来设计和进行这种修改。
本发明的组合物可包含按组合物总重量计约0.005％至2.0％的防腐剂。防腐剂用于防止在暴露于环境中的污染物的情况下变质。
施用/给药
本发明组合物的给药方案可影响有效量的构成。例如，本发明的疫苗、多肽和/或核酸可以以单剂量、以几个分开的剂量以及可以每天或依次给予交错剂量的形式施用至受试者(即哺乳动物)，或该剂量可以连续输注，或者可以是推注。此外，根据治疗或预防情况的紧急情况，可以按比例增加或减少剂量。
将本发明的组合物施用于受试者，优选地哺乳动物，更优选地人，可以使用已知的程序，以有效治疗受试者疾病的剂量和时间段进行。实现预期结果所必需的组合物的有效量会有所不同，并取决于诸如待治疗或预防的疾病、年龄、性别、体重、状况、一般健康状况和待治疗受试者的既往病史的因素，以及医学领域中众所周知的类似因素。在具体实施方式中，将组合物配制成剂量单位形式是特别有利的，以便于给药和剂量的均匀性。如本文所使用的剂量单位形式是指适合作为待治疗对象的单位剂量的物理离散单位；每个单元含有预定量的治疗化合物，经计算可产生与所需药物载体相关的所需治疗效果。本发明的剂量单位形式由(a)组合物的独特特征和要表达的异源蛋白质以及要实现的特定治疗效果决定并直接依赖于(a)。
在一些实施方式中，所施用的疫苗的量可以取决于例如待治疗的受试者、受试者的免疫系统产生所需免疫反应的能力和/或所需的保护程度。
在其他实施方式中，本发明的疫苗的施用可以根据任何合适的疫苗接种方案，例如从第0天起的第0天、1个月、4个月和12个月。在其他实施方式中，本文所述的疫苗也可以以单剂量方案或多剂量方案给予，其中主要的疫苗接种过程可能是1-10次单独的剂量，然后在随后的时间间隔给予其他剂量，以维持和/或加强免疫反应，例如，在1-4个月时接种第二剂，并且如果需要，几个月后接种后续剂量(一种或多种)。在一些实施方式中，合适的免疫计划的其他实例包括但不限于：(i)0个月、1个月和6个月，(ii)0天、7天和1个月，(iii)0个月和1个月，(iv)0个月和6个月，(v)0个月、1个月和2个月，或足以引发预期赋予保护性免疫和/或减轻疾病症状或减轻疾病严重程度的所需免疫反应的其他方案。
在一个实施方式中，本发明疫苗的施用可以根据有效产生抗原特异性免疫反应的任何合适的疫苗接种方案和/或剂量(一种或多种)。在一些实施方式中，抗原特异性免疫反应包括B和/或T细胞反应。在其他实施方式中，抗原特异性免疫反应包括施用单剂量(无加强剂量)。在一些实施方式中，可以施用第二剂(加强剂)疫苗。在其他实施方式中，可以施用额外的剂量。
在一些实施方式中，本发明疫苗的施用可以根据有效的任何合适的疫苗接种方案和/或剂量(一种或多种)，使得受试者在疫苗的第一剂或第二剂(加强剂)之后表现出至少60％、至少70％、至少80％、至少85％、至少90％或至少95％的血清转化率。
在其他实施方式中，本发明的疫苗的施用可以根据在受试者中有效产生抗原特异性免疫反应的任何合适的疫苗接种方案和/或剂量，其中在受试者相对于对照增加至少1个对数。
在一些实施方式中，对照是在未施用本公开内容的疫苗的受试者中产生的抗抗原抗体滴度。在其他实施方式中，对照是在已施用减毒活疫苗或灭活疫苗；重组或纯化的蛋白质疫苗；或病毒样颗粒疫苗的受试者中产生的滴度。
在一个实施方式中，在受试者中产生的滴度相对于对照增加1-3个对数。在另一个实施方式中，在受试者中产生的滴度相对于对照增加至少2倍。在其他实施方式中，在受试者中产生的滴度相对于对照增加至少3、4、5、10或更多倍。在一个实施方式中，在受试者中产生的滴度相对于对照增加至少10倍。在另一个实施方式中，在受试者中产生的滴度相对于对照增加2-10倍。
在一些实施方式中，本公开内容的疫苗以有效量(例如，有效诱导免疫反应的量)施用于受试者。在一些实施方式中，有效量是相当于重组蛋白疫苗的标准护理剂量减少至少2倍、至少4倍、至少10倍、至少100倍、至少1000倍的剂量，其中在受试者中产生的抗抗原抗体滴度与在施用标准护理剂量的重组蛋白疫苗、纯化的蛋白疫苗、减毒活疫苗、灭活疫苗或VLP疫苗的对照受试者中产生的抗抗原抗体滴度相当。在一些实施方式中，有效量是相当于重组蛋白疫苗、纯化蛋白疫苗、减毒活疫苗、灭活疫苗或VLP疫苗的标准护理剂量减少2-1000倍的剂量。
在其他实施方式中，施用的疫苗的有效量包含总剂量约0.1μg至约1000μg，示例性地、约1μg至约900μg、约5μg至约700μg、约10μg至约500μg、约15μg至约300μg、约20μg至约200μg，和约25μg至约100μg的疫苗或活性成分(例如，VLP、病毒体、病毒载体、抗原或核酸分子)，在一些实施方式中，有效量为约25μg、约50μg，或约100μg的总剂量。在其他实施方式中，有效量为不小于(NLT)约1μg、约5μg、约10μg、约15μg、约20μg、约25μg、约30μg、约35μg、约40μg、约45μg、约50μg、约55μg、约60μg、约65μg、约70μg、约75μg、约80μg、约85μg、约90μg、约95μg或约100μg的总剂量。在其他实施方式中，有效量为约0.1μg至约10μg的总剂量。
在一些实施方式中，疫苗的剂量基于DNase-抗性颗粒(DRP)的定量。在一个实施方式中，DRP相当于包壳的载体基因组。在一些实施方式中，受试者接收至少一剂(例如，在时刻零第一剂)约105至1020约DRP，示例性地、约106至1018、约107至1017、约108至1016、约109至1015、约1010至1014，和约1011至1013DRP的疫苗。在其他实施方式中，受试者接收至少一剂(例如，在时刻零第一剂)至少约105、至少约106、至少约107、至少约108、至少约109、至少约1010、至少约1011、至少约1012、至少约1013、至少约1014、至少约1015、至少约1016、至少约1017、至少约1018、至少约1019或至少约1020DRP。
在一些实施方式中，有效量是施用至受试者总共1、2、3、4、5或更多次的剂量。
在一些实施方式中，本公开内容的疫苗的功效或有效性等于或大于约60％。可以使用例如本领域已知的标准分析和方案来评估疫苗功效或有效性。在一个实施方式中，疫苗的效力或有效性为至少约60％、至少约65％、至少约70％、至少约75％、至少约80％、至少约85％或至少约90％。
在一些实施方式中，疫苗使受试者针对冠状病毒(例如，SARS-CoV-2病毒)免疫长达6个月、1年或2年。在一些实施方式中，疫苗使受试者针对冠状病毒(例如，SARS-CoV-2病毒)免疫至少约6个月、至少约1年、至少约2年、至少约3年、至少约4年或约5年至约10年，或更长。
施用途径
本领域技术人员将认识到，尽管可以使用多于一种途径进行施用，但特定途径可以提供比另一种途径更直接和更有效的反应。本发明的任何组合物的施用途径包括吸入、口服、经鼻、直肠、肠胃外、舌下、透皮、经粘膜(例如，舌下、舌、(经)颊、(经)尿道、阴道(例如，经阴道和阴道周围)、鼻(内)和(经)直肠、膀胱内、肺内、十二指肠内、胃内、鞘内、皮下、肌肉内、皮内、动脉内、静脉内、支气管内、吸入、电穿孔和局部施用。
试剂盒
在一些实施方式中，提供了用于治疗、预防或改善如本文所述的给定疾病、紊乱或病症或其症状的试剂盒，其中试剂盒包括：a)本文所述的化合物或组合物；和任选地b)如本文所述的另外的药剂或疗法。该试剂盒还可包括使用该试剂盒治疗、预防或改善疾病、紊乱或病症的说明书或标签。在又一些实施方式中，本发明延伸至用于如本文所述的给定疾病、紊乱或病症或其症状的试剂盒测定。例如，这种试剂盒可以包含来自PCR或其他核酸杂交技术(微阵列)的试剂或用于基于免疫学的检测技术(例如，ELISpot、ELISA)的试剂。
治疗方法
在一方面中，本发明包括在有需要的受试者中产生针对SARS-CoV-2病毒的免疫反应的方法。在另一方面中，提供了向受试者接种针对SARS-CoV-2病毒的疫苗的方法。在仍另一方面中，提供了一种在受试者中提供针对SARS-CoV-2病毒的免疫力的方法。在仍另一方面中，在有需要的受试者中治疗和/或预防与SARS-CoV-2病毒相关的疾病或紊乱的方法。在一些实施方式中，该方法包括向受试者施用有效量的本文所述的重组病毒。在一些实施方式中，该方法包括向受试者施用有效量的本文所述的疫苗。
在一些实施方式中，与SARS-CoV-2病毒相关的疾病或紊乱是呼吸道疾病。在一些实施方式中，与SARS-CoV-2病毒相关的疾病或紊乱是冠状病毒病。在一些实施方式中，与SARS-CoV-2病毒相关的疾病或紊乱是COVID-19。
可以以适合待治疗(或预防)的疾病的方式施用包括本发明的疫苗的药物组合物。施用的数量和频率将由患者的病情、患者疾病的类型和严重程度等因素决定，但合适的剂量可通过临床试验确定。
本发明的疫苗的施用可以本领域技术人员已知的任何方便的方式进行。本发明的疫苗可以通过喷雾吸入、注射、摄取、输血、植入或移植施用至受试者。本文所述的组合物可经动脉、皮下、皮内、瘤内、结内、髓内、肌肉内、通过静脉内(i.v.)注射或腹膜内施用至患者。
考虑施用本发明的药物组合物的受试者包括但不限于人类和其他灵长类动物、哺乳动物和鸟类，包括商业相关的哺乳动物和鸟类，比如牛、猪、马、羊、鸡、鸭、猫、狗和雪貂。
在一些实施方式中，受试者是家养动物。在一些实施方式中，受试者是家养宠物。在一些实施方式中，动物是圈养动物，例如饲养在展览或动物园中的动物。在一些实施方式中，动物是家畜。在一些实施方式中，受试者是易受SARS-CoV-2感染的动物并且可以是SARS-CoV-2病毒的宿主。在一些实施方式中，受试者是猫科动物。在一些其他实施方式中，受试者是犬科动物。在一些实施方式中，受试者是鼬科的成员，比如黄鼠狼、鸡貂、白鼬、貂、貂、獾、水獭或雪貂。在一些实施方式中，受试者是猫。在一些其他实施方式中，受试者是狗。在一些实施方式中，受试者是雪貂。
在一方面，提供了一种在受试者中预防SARS-CoV-2感染或提供对SARS-CoV-2的免疫力的方法，该方法包括向受试者施用本文所述的SARS-CoV-2疫苗，其中该受试者是选自猫、狗和雪貂的家养宠物。特别是，猫科动物和雪貂已被确定为SARS-CoV-2的潜在宿主(J.Shi等人，Science 10.1126/science.abb7015(2020))。猫是美国公民最喜爱的宠物之一。在美国，三分之一的家庭拥有宠物猫，平均每个拥有猫的家庭拥有2.2只猫。然而，宠物猫只是该国猫科动物总数的一部分，估计约为7650万只。
目前，建议为猫、狗和雪貂接种狂犬病疫苗。CDC指南建议所有狗、猫和雪貂都应根据产品标签说明(www.cdc.gov/rabies/specific_groups/veterinarians/vaccination.html)接种和重新接种狂犬病疫苗。如果以前接种过疫苗的动物逾期进行加强免疫，则应重新接种。加强免疫后立即认为该动物目前已接种疫苗，应根据所用疫苗的标记持续时间安排接种计划。
现有的用于猫、狗或雪貂的RABV疫苗可以用基于狂犬病病毒的SARS-CoV-2疫苗代替，以诱导保护免于这两种疾病。因此，在一方面，提供了一种在受试者中提供对狂犬病和SARS-CoV-2相关疾病的免疫力的方法，其中受试者是猫、狗或雪貂。该方法包括向受试者施用包括基于狂犬病病毒的SARS-CoV-2疫苗的疫苗的步骤。在另一方面，提供了用于猫、狗或雪貂的疫苗，该疫苗包括基于狂犬病病毒的SARS-CoV-2疫苗。在一些实施方式中，疫苗包含重组狂犬病病毒，该重组狂犬病病毒包含SARS-CoV-2刺突蛋白(S)或其部分。在一些实施方式中，疫苗包括重组狂犬病病毒，其包括(a)病毒的糖蛋白(G)或其部分和(b)SARS-CoV-2刺突蛋白(S)或其部分的融合物。在一些实施方式中，疫苗包括灭活病毒。在具体实施方式中，疫苗包括佐剂。在一些实施方式中，疫苗被配制用于施用于猫。在一些其他实施方式中，疫苗被配制用于施用于狗。在其他实施方式中，疫苗被配制用于施用于雪貂。
应当理解，可用于本发明的方法和组合物不限于实施例中阐述的特定制剂。提出以下实施例是为了向本领域普通技术人员提供关于如何制备和使用本发明的细胞、扩增和培养方法以及治疗方法的完整公开和描述，并且不旨在限制发明人所认为的他们的发明的范围。
除非另有说明，否则本发明的实践采用分子生物学(包括重组技术)、微生物学、细胞生物学、生物化学和免疫学的常规技术，这些技术完全在本领域技术人员的能力范围内。这种技术在文献中得到了充分解释，比如，“Molecular Cloning:A Laboratory Manual”，第四版(Green,M.R.&Sambrook,J.,Cold Spring Harbor Laboratory Press,2012)；“Oligonucleotide Synthesis,a practical approach”(Paselk R.A.，Gait编，M.J.,IRLPress,Oxford,1984)；“Culture of Animal Cells:A Manual of Basic Technique andSpecialized Applications,第六版”(Freshney,R.I.,John Wiley&Sons,Inc.,2010)；“Methods in Enzymology”(第152卷，Guide to Molecular Cloning Techniques,Bergerand Kimmel,Eds.,San Diego:Academic Press,Inc.,1987)；“Handbook of ExperimentalImmunology”(Herzenberg L.A.,Weir,D.M.,Blackwell,C.,Wiley,1997)；“Gene TransferVectors for Mammalian Cells”(Miller and Calos,eds.,Cold Spring HarborLaboratory,Cold Spring Harbor,1987)；“Short Protocols in Molecular Biology”(Ausubel,F.M.等人，ed.,John Wiley&Sons,2002)；“Polymerase Chain Reaction:Principles,Applications and Troubleshooting”(Babar,M.E.,publisher VDM VerlagDr.Müller,2011)；“Current Protocols in Immunology”(Colligan,J.E.等人，ed.,Greene Pub.Associates and Wiley-Interscience,2002)。这些技术适用于本发明的多核苷酸和多肽的生产，因此可以在制造和实践本发明时考虑。用于具体实施方式的特别有用的技术将在以下部分中讨论。
实施例
现在参考以下实施例描述本发明。提供这些实施例仅用于说明的目的，并且本发明不限于这些实施例，而是涵盖由于本文提供的教导而显而易见的所有变化。
实施例1
表达Covid S或Covid-S1的RABV、VSV和MV
现在描述实施例1-5中使用的材料和方法。
生成针对COVID-19的基于RABV、VSV和MV的疫苗
SARS-CoV-2刺突蛋白(S)用于生成本文所述的针对COVID-19的疫苗。SARS-CoV-2导致Convid-19。S1结构域(S蛋白的受体结合位点)被整合到狂犬病病毒(RABV)和水疱性口炎病毒(VSV)的基因组中。对于这两种构建体，它们的天然G蛋白的一部分用于促进S1结构域的整合。发现全长SARS-CoV-2S会干扰病毒生长(VSV或RABV G)，使其几乎不可能生产疫苗；因此改为使用S1结构域。
还制备了三种表达全长SARS-CoV-2刺突蛋白(S)的基于麻疹病毒(MV)的疫苗。这三种病毒从基因组的第2、3或6位表达SARS-CoV-2刺突蛋白(S)。先前的研究表明，对于麻疹病毒，外源基因的表达位置对于MV复制和转基因的免疫原性很重要。然而，这取决于抗原，因此制备了三种不同的病毒。
在动物中测试针对COVID-19的基于RABV、VSV和MV的疫苗
在下一阶段，疫苗在动物中进行测试。两种病毒的受体都被认为是ACE 2。将要使用的模型是仓鼠或小鼠(表达ACE2的转基因或感染表达ACE2的腺病毒的野生型)。
活病毒和死病毒都经过测试。免疫后，小鼠或仓鼠受到SARS-CoV-2(Convid-19)的攻击。
2019-nCoV疫苗的免疫学参数
诱导体液和细胞免疫反应的参数将在肌肉内注射接种小鼠(包括2019-nCoV攻击)和非人灵长类动物(NHP)(免疫原性)之后进行研究。ELISA和病毒中和试验(VNA)将用于分析体液免疫反应。这些试验是为小鼠和NHP开发的，并且基于有限的篇幅，下面仅简要介绍。以前的经验将用于开发这种试验，特别是在冠状病毒领域。
用于检测2019-nCoV S1和载体RABV G特异性体液反应的ELISA测定
制备针对2019-nCoV S1或RABV G的高度纯化抗原。如以前针对MERS-CoV所述，从转染的293T细胞上清液中纯化带有HA标记的可溶性蛋白(17)。可以制备足以用于大量测定板的mg范围内的纯化蛋白质。Schnell实验室定期生产大约10-20mg纯化的RABV G。
为了确定对2019-nCoV S或RABV G的抗体反应，将使用纯化的S1或G蛋白开发间接ELISA(26)，但未发表。来自接种疫苗的小鼠或NHP的血清将以不同的稀释度用于随时间确定EC50。每种疫苗抗原的血清IgM和IgG抗体(NHP的总IgM、IgG、IgG1、IgG2，B6的IgG2c和IgG1)将通过托马斯杰斐逊大学(Thomas Jefferson University)(TJU)实验室中成熟的合格ELISA进行测量。在进行ELISA测定之前，我们将对每种疫苗抗原的测定进行资格鉴定。我们的ELISA平台将标准化，以通过表征测定变异性、确定定量限、确定阳性和阴性质量对照范围以及确定测定接受标准来测量疫苗抗原特异性的血清IgG。TJU在使用该平台进行ELISA测定的资格鉴定和执行方面拥有丰富的经验，并且还计划将其用于未来的临床研究。人体临床样本的测定将经由IQVIA转移给承包商。
RABV病毒中和测定(VNA)在TJU实验室中对小鼠、猴子和人类血清是很成熟的(12、27、28)。使用WHO内部标准，可以确定免疫接种所达到的国际单位(I.U.)。血清中0.5I.U或更多的存在被认为与免受狂犬病相关。MERS-CoV的VNA是在马里兰大学弗里曼实验室建立的。弗里曼博士有来自华盛顿州患者CDC的病毒样本。他还在使用他的传染性克隆重建多个菌株并合成完整的基因组。
细胞反应
根据先前对冠状病毒的研究，体液反应很可能是保护的关键，但作为研究部分，将使用合格的13色ICS测定在脾细胞或冷冻保存的NHP PBMC中测量疫苗抗原特异性T细胞的频率和细胞因子表达谱。为了评估持久抗体反应的潜力，将进行抗原特异性IgG+ELISPOT分析，以检测加强免疫后28天骨髓中分泌抗体的浆细胞。
现在描述实验的结果。
表达Covid S或Covid-S1的RABV、VSV和MV
产生了来自基因组第2、3和6位的表达密码子优化的Covid-S1的VSV、表达密码子优化的Covid-S1的RABV和表达密码子优化的Covid-S的MV(图1-5；图17-21)。
图6显示了表达嵌合S1-G融合蛋白的RABV(RABV-COV19-S1)和VSV(VSV-COV19-S1)的表征。蔗糖梯度纯化后纯化的病毒体的SDS-PAGE分析。字母表示VSV或RABV L、G、N、P和M蛋白的位置。左边的数字表示分子量标准的大小。标明了Covid-19 S1蛋白。对照显示VSV和RABV病毒体。
实施例2
基于狂犬病疫苗的针对2019-nCoV的二价疫苗
为MERS-CoV和SARS-CoV开发的技术可以转移到2019-nCov。大多数疫苗设计都集中在主要的免疫优势抗原，位于病毒体表面的刺突(S)蛋白，它用作MERS-CoV受体二肽基肽酶4(DPP4，也称为CD26)的配体(1)。与MERS-CoV S类似，2019-nCoV S是一种跨膜糖蛋白，很可能与MERS-CoV S一样被弗林蛋白酶切割成S1和S2结构域(2)。不意欲受理论的束缚，据信，对于其他冠状病毒比如MERS-CoV，响应感染或接种MERS-CoV S而产生的病毒中和抗体(VNA)将在体外中和病毒感染并在小鼠疾病模型中保护肺部免受感染(3-6)。
本文描述的是一种基于弹状病毒的疫苗，它提供了可能被证明是有效的2019-nCoV疫苗所需要的特征组合。已经针对几种人类病原体开发了以弹状病毒为载体的候选疫苗(7)。最近，RABV和VSV都已成功用作埃博拉病毒(EBOV)疫苗，并且这两种方法要么接近临床试验(RABV)，要么已经完成2期临床试验(VSV)(8、9)。化学灭活的RABV疫苗被广泛使用且对人类安全；每年约有1亿剂灭活RABV疫苗用于人体，证明了极好的安全性(10)。活的和化学灭活的RABV疫苗都被证明对动物是安全的。在一项概念验证研究中，减毒活和灭活的RABV-EBOV均已成功用于对抗EBOV，并且该疫苗将于2020年进入1期临床试验(11-14)。
基于充分表征的MERS-CoV疫苗构建减毒RABV 2019-CoV(图12和(15))。
将编码2019-nCoV S1蛋白的核苷酸序列插入到cBNSP333载体中(图12；图18“BNSP333-COVID19-S1-RVG”)。2019-nCoV S是一种锚定在2019-nCoV病毒体膜上的糖蛋白，它从病毒表面突出，作为易感细胞的配体，并且因此是一种主要的免疫原。使用的BNSP333疫苗载体源自减毒RABV毒株SAD-B19(16)。对亲本菌株进行了一些修改，以提高安全性并最大限度地表达外源基因。先前已表明可以将外源基因稳定地导入该载体中(14、17-20)。此外，表明，从RABV N和P基因之间的位置表达外源抗原，以及目标基因的人类细胞密码子优化，导致外源抗原的最高表达水平(14)。此外，在RABV糖蛋白(G)内的第333位(333R->333E)用谷氨酸替换精氨酸可进一步降低已经高度减毒的载体的致病性(19)。这种改进的载体已成功用于生产针对几种新出现的人畜共患病毒性疾病(如EBOV和Henipaviruses)的候选疫苗(14，21)。然而，发现全长CoV S的表达会抑制RABV G蛋白的表达并显著降低病毒滴度。然而，与RABV G的C-末端部分融合的S1的表达导致RABV G-MERS-CoV-S1融合蛋白的有力整合。RABV G-2019-nCoV-S1疫苗类似于MERS-CoV331-S1＝MERSRAB)，其表达与截短的RABV糖蛋白融合的2019-nCoV S的N-末端750个氨基酸，其包括RABV G的胞外结构域(ED)的31个氨基酸和RABV G的完整CD和跨膜结构域，以允许嵌合糖蛋白整合到RABV病毒体中。嵌合的2019-nCoV S1/RABV G蛋白利用原始的2019-CoV ER易位序列(SS)，并通过密码子优化的cDNA片段的PCR生成(图12)。
以下所有结果均来自先前使用MERS构建体(MERSRAB)的研究。对于MERSRAB，传染性病毒被回收。表达S1片段的新病毒在Vero细胞上生长到与已获准用于人类疫苗生产的对照病毒BNSP333相似的滴度，大约为108FFU/mL。表达S1的BNSP333 RABV被命名为MERSRAB，并且下面列出的动物功效研究来自该构建体。基于RABV的MERSRAB疫苗在三种不同的动物模型中证明是有效的：用表达人类hDPP4(MERS-CoV的受体)的Ad5病毒转导的小鼠、表达人类hDPP4的CRISPR-CASc小鼠和羊驼(骆驼科)。
MERSRAB在小鼠中具有免疫原性，并且可防止MERS-CoV的攻击。为了分析MERSRAB的免疫原性(图12和图13)，我们在接种后第0、7和21天用10μg对照病毒FILOAB1(埃博拉病毒疫苗，第1组)、用10μg MERSRAB(第2组和第3组)或PBS(第4组)免疫4组BALB/c小鼠(每组5只小鼠)。我们通过抗原特异性ELISA跟踪针对RABV G和MERS-CoV S的免疫反应。抗原特异性IgG反应随时间并在每次免疫后增加；他们在第三次接种后达到了针对RABV G和MERS-CoV S的高抗体水平。仅在第2组和第3组中检测到MERS-CoV-S特异性免疫反应，但在第1-3组中检测到RABV G特异性IgG。模拟免疫的第4组动物均未表现出针对RABV G或MERS-CoV S蛋白的免疫反应，这证实了ELISA测定的特异性。虽然已知RABV G特异性ELISA滴度可预测针对RABV攻击的保护作用(22-24)，但是尚不清楚MERS-CoV S定向抗体的保护能力。因此，在所有四组免疫小鼠的第35天，针对血清中的MERS-CoV进行了VNA。在第1组(FILORAB1)或模拟(PBS)免疫小鼠(第4组)的小鼠血清中检测到低水平的MERS-CoV中和抗体，但是用MERSRAB(第2组和第3组)免疫的小鼠血清以1:1280和1:5120之间的血清稀释度中和了MERS-CoV。这表明在BNSP333-S1疫苗接种小鼠中产生了高水平的抗MERS-CoV中和抗体。
RABV-MERS疫苗的功效测试是使用腺病毒-hDPP4转导的小鼠模型进行的(25)。所有四组小鼠都进行了转导，并且在第五天后，用MERS-CoV鼻内(IN)攻击第1、2和4组的小鼠用1×105pfu/小鼠(菌株Jordan-n3/2012)。在攻击后四天，对小鼠实施安乐死，并解剖它们的肺，均质化，并通过qRT-PCR和病毒斑块测定法测定病毒载量。对于BNSP333-S1免疫小鼠，基因组和mRNA均降低到与未由表达Ad5的hDPP4转导的小鼠中发现的相似的背景水平。此外，用BNSP333-S1进行免疫将肺中的病毒载量降低到低于测定检测的水平。在下一步中，基于RABV的疫苗MERSRAB在CRISPR-CAS生成的转基因小鼠模型(表达人类hDPP4的小鼠)中进行了测试。在第0、7和28天或仅在第0天和第28天，用10μg对照病毒FILORAB(空载体，狂犬病组对照疫苗)、10μg MERSRAB(MERS/狂犬病双疫苗)免疫10只转基因小鼠(图13)。针对RABVG和MERS-CoV S的免疫反应之后是抗原特异性ELISA。抗原特异性IgG反应随时间并在每次免疫后增加；他们在第二次或第三次免疫后达到了针对RABV G和MERS-CoV S的高抗体水平。仅在第1组和第2组(MERS-狂犬病)中检测到MERS-CoV-S特异性免疫反应，但在所有三组中均检测到RABV G特异性IgG。
虽然已知RABV G特异性ELISA滴度可预测针对RABV攻击的保护作用(22-24)，但是MERS-CoV S定向抗体的保护能力尚未得到很好的表征。因此，转基因小鼠在第56天用致病性MERS-CoV攻击。如图14A中所显示，所有免疫小鼠在感染中存活，而40％的模拟免疫动物死于感染。如图所示，所有接受一到两次MERSRAB接种的小鼠都完全免于体重减轻。此外，免疫确实将病毒载量降低到检测不到的水平(图14A-14C)，而MERS-CoV在空载体免疫动物中复制到非常高的水平(图14C)。这也可以在图15中看到。MERSRAB接种2次或3次可保护动物免于肺部病毒复制，并且在攻击后第3天检测到免疫小鼠肺中的MERS-CoV抗原，第6天未检测到抗原。相比之下，仅用载体免疫动物的肺组织，在两个时间点都可以检测到大量的组织损伤和病毒抗原，这与图14A-14C所示的病毒载量一致。
最后，为了确定预防从宿主物种传播给人类的潜力，下一项研究测试了MERSRAB疫苗在骆驼科动物中是否有效。简而言之，将一组五只羊驼用107个病灶形成单位(ffu)的活MERSRAB肌肉内注射或鼻内免疫或用100μg或300μg灭活的MERSRAB疫苗肌肉内注射免疫。在56天后，用MERS-CoV对动物进行鼻内攻击，并随着时间的推移采集鼻拭子以分析病毒脱落。用活的或灭活的MERSRAB疫苗免疫后未检测到疾病。如图15所示，两次接种100或300μg的疫苗几乎完全抑制了受感染动物的病毒脱落。
灭活的RABV作为一种高效安全的疫苗已有30年的悠久历史。综上所述，上述结果清楚地表明，基于灭活的RABV颗粒的疫苗是对抗2019-nCoV感染的有力候选者。
此外，佐剂的使用可能会在一次疫苗接种后产生临床益处。适当的佐剂选择可能对于设计安全有效的nCoV疫苗至关重要。值得注意的是，当SARS疫苗与铝盐佐剂一起配制时，认为疫苗增强的嗜酸性肺病会加剧，而当相同的SARS疫苗与Advax-SM佐剂(多糖颗粒与强效TLR9激动剂组合的制剂)一起配制时，可以完全预防。将Advax添加到广泛的疫苗中会带来显著的好处，包括与更高抗体滴度相关的增强保护、增加B细胞受体亲和力成熟、IgG亚型多样化、增强记忆CD4和CD8 T细胞反应以及抗原剂量节省(sparing)。Advax佐剂已被证明可以在新生幼崽中以单剂量提供保护性免疫。Advax佐剂的一个特别优势是，它们已在人体临床试验中与许多不同的抗原结合被证明具有安全的和良好的耐受性，从而有助于将成功的疫苗从临床前研究快速转化为人体试验。
佐剂增加疫苗的免疫力，并且也可以改变抗体同种型。先前对于狂犬病病毒平台显示，在角鲨烯
Figure GDA0004183787440001991
中添加MPLA会增加总体液反应以及Th1/TH2偏置。对于大多数病毒疫苗，已显示TH1偏置是有益的。已经证明TH1偏置对于FILORAB1疫苗对EBOV的强有力保护是必要的。对于2019-nCoV疫苗，可以使用2％角鲨烯中的MPLA 3D(6-acyl)
Figure GDA0004183787440001992
以及具有增强免疫力和诱导TH1反应的潜力的佐剂(例如，CpG-寡脱氧核苷酸)。
实施例3
临床前研究
以下描述了测试2019-nCoV疫苗的临床前研究：
1)最关键的是在动物模型(小动物和NHP)中测试功效的能力，这将与拟议的1期临床试验同时进行，以评估安全性和免疫原性以及潜在的不利影响，比如疫苗接种后感染的增强。由于2019-nCoV和SARS-CoV似乎利用相同的受体，人类ACE2转基因小鼠可能是2019-nCoV的合适模型，然而尚未表征对该模型的评估，并且确认该模型的实验正处于计划阶段。NHP动物建模预计将于2020年3月中旬开始。将评估食蟹猴、非洲绿猴、普通狨猴和恒河猴在气管内、小颗粒喷雾或大颗粒喷雾后的疾病发展。IRF-Frederick拥有MERS、埃博拉病毒、尼帕病毒和牛痘喷雾模型的经验。独特的带有计算机断层扫描的正电子发射断层扫描(PET/CT)将被纳入动物模型以测量疾病进展，因为非人灵长类动物模型预计是亚致死的。CT之前已用于证明疫苗功效和补充小分子对抗功效测试(www.ncbi.nlm.nih.gov/pubmed/26218507和www.ncbi.nlm.nih.gov/pmc/articles/PMC5640857/)。
CT还被用于评估MERS恒河猴模型中的MERS单克隆抗体，以及评估MERS普通狨猴模型中的疾病进展(www.ncbi.nlm.nih.gov/pubmed/26828465和www.ncbi.nlm.nih.gov/pubmed/26342468)
2)在TJU通过生化和病毒学测定表征2019-nCoV疫苗
3)在GLP下在BBIL回收疫苗
4)建立针对2019-nCoV毒株的VNA。为BSL2建立假型VNA测定
5)针对2019-nCoV S的ELISA——类似于之前开发的针对MERS-CoV S的ELISA。
6)疫苗和佐剂的配制。
实施例4
临床研究
以下描述了测试2019-nCoV疫苗的临床研究：
基于来自临床前研究的信息，动物模型的疗效将与拟议的1期临床试验同时进行，以评估在人体受试者中的安全性和免疫原性。值得注意的是，我们之前与FDA就针对EBOV的类似的基于灭活的狂犬病毒疫苗的讨论(IND前)同意不需要基于应用于超过1亿人的灭活狂犬病疫苗的安全历史进行毒性研究。1期研究如果成功，随后将进行2期临床试验，以增加年龄范围的上限(因为老年人患有最严重的2019年nCoV疾病)并扩大群组规模以获得与免疫反应相关的统计有效数据。
目标人群：18至55岁(包括18和55岁)的健康美国成年人。
临床试验设计：1期临床试验
1期试验是一项随机、观察者不知情的、剂量递增研究，以评估基于灭活的狂犬病毒(RABV)的2019-nCoV候选疫苗(RABV-nCoV)的安全性、反应原性和免疫原性。临床试验将在18至55岁的健康成年人中在巴尔的摩马里兰大学(University of Maryland)的一个地点进行。入组(enrolment)将分阶段进行。我们将与群组1中的8名受试者在一起。如果在第一剂疫苗后第8天没有满足暂停规则，那么群组2可以在群组1完全入组后立即开始，没有暂停。第3组和第4组将在审查第2组中的第8天数据后同时进行(假设没有满足暂停规则)。重要的是，我们将包括一个使用佐剂的单剂量组，该组将与100ug组同时开始(因此第3组和第4组将在通过第8天从第2组获得所有安全数据后一起入组)。该研究由四个群组组成，共64名受试者(每个群组中有2个狂犬病疫苗对照和14个研究疫苗)。所有研究注射将通过肌肉注射(IM)途径施用。在表1中列出治疗分配。
表1：nCoVRAB疫苗I期范围安全性研究的治疗分配
Figure GDA0004183787440002011
主要目标：评估无佐剂nCoVRAB的2剂次方案和在第0天和第28天肌肉内递送佐剂候选疫苗的1剂次方案的安全性和耐受性。
次要目标：在第0、28、56、120天(在第56天无佐剂和第28天加佐剂进行主要测量)时评估nCoVRAB候选疫苗在第0天和第28天(或第0天加佐剂)的免疫原性。
主要终点：在以下时间点经历任何与研究注射相关的不良事件、请求的系统性事件或严重不良事件的研究参与者的数量和百分比：
·在每次研究注射后7天内的请求事件。
·在最后一次研究注射后28天内(第56天)的未经请求的事件。
·严重不良事件(SAE)持续56天，并且在第56天、第120天和第365天进行脚本访谈。
·提前终止研究注射的参与者人数和终止原因。
次要终点：通过ELISA对2019-nCoV和狂犬病GP测量的免疫原性。
·在第120天内每个时间点的nCoV S1和nCoV S1和RABV G特异性血清IgGELISA滴度：几何平均抗体滴度(GMT)和受试者血清转化百分比(比基线上升4倍)。
·在第120天内的nCoV和狂犬病中和抗体滴度：GMT和受试者血清转化百分比(比基线上升4倍)；对于狂犬病，滴度>0.5IU/ml(保护相关性)的受试者百分比。
探索(未来研究)
·抗原特异性血清IgM、IgA和IgG亚类(选定的时间点)。
·T细胞反应(疫苗接种后的Th1、Th2和Th17细胞因子和趋化因子)。
·B细胞反应(疫苗接种后抗原特异性浆母细胞和记忆B细胞的数量)。
·IgG亲和力。
2期临床试验
该临床试验被提议作为一项2期、随机的、观察者不知情的、单抗原水平研究，以评估两剂狂犬病载体nCoVRAB疫苗(或一剂佐剂方案)的安全性和免疫原性。鉴于当前NCoV爆发的人口统计数据，我们将把年龄上限扩大到70岁。志愿者将在注射前和疫苗接种后第1、3、7、14和28天接受安全性、反应原性和免疫原性评估。临床试验将在美国2个地点——马里兰州巴尔的摩和另外一个地点，在符合所有纳入标准且无排除标准的健康成人中进行。共有200名成年人将按年龄划分连续入组。所有研究注射将通过肌肉内(IM)途径施用。下表2概述了治疗分配。
表2：nCoVRAB疫苗首次剂量范围安全性研究的治疗分配*
Figure GDA0004183787440002021
*用于本研究的总抗原剂量、抗原组分的相对浓度将取决于1期临床试验的结果。
主要目标：评估领先的nCoVRAB候选疫苗在0天和28天时通过肌肉注射的安全性和耐受性。
次要目标：在第7、14、28、35、42、56、90、120、180和365天评估nCoVRAB候选疫苗在第0天和第28天肌肉内注射时的免疫原性。
主要终点：在以下时间点经历任何研究注射相关不良事件或严重不良事件的研究参与者的数量和百分比：
·在每次研究注射后7天的请求事件。
·在最后一次研究注射(第56天)后28天内的未经请求的事件。
·严重不良事件(SAE)持续56天，并且在第56天、第120天和第365天进行脚本访谈。
·提前终止研究注射的参与者人数和终止原因。
次要终点/探索性终点见上文(1期临床试验)
实施例5
制造
在2019年新型冠状病毒(2019-nCoV或SARS-CoV-2)突发公共卫生事件的背景下，开发2019-nCoV疫苗的考虑必须在制造速度和技术可行性与临床安全性和免疫原性考虑之间取得平衡。最终，制造必须是规模可扩大的，并且交付简单，以便在最短的时间内达到最大的人数。合作者Bharat Biotech Ltd(BBIL)是一家大型疫苗制造商，能够迅速将这种疫苗的生产规模扩大到几百万剂。依靠现有技术最大限度地提高开发的可行性和速度是实现这一目标的最佳方法。
下表总结了BBIL在为国际供应品定位许可疫苗方面的经验。
制造和扩大规模/基础设施和设备方面的经验
BBIL拥有多个生产病毒、细菌和重组药物物质的设备。BBIL是印度最大的病毒疫苗制造商。在病毒疫苗的制造方面，它拥有利用Vero、MDCK细胞系和细胞工厂、微载体和发酵罐等平台的专业知识。有5个病毒散装生产设备，包括一个中试工厂，这些设备的能力范围从50L到1000L发酵并通过TFF、凝胶渗透和亲和层析纯化。这些自动化设备可以生产多达5亿剂疫苗。
BBIL还具有生产重组和细菌药物物质的能力，并且这些药物在6个设施中生产，包括一个中试工厂，BBIL的专业知识范围包括使用重组大肠杆菌、酵母、产生毒素的细菌，比如白喉、破伤风和全细胞细菌，即百日咳和霍乱。这些生物体从50L到1500L发酵罐进行处理，并且随后使用离子交换、凝胶渗透色谱进行纯化。
对于药物产品，所有散装产品在灌装机中被装在容量为0.5ml到25ml的小瓶、PFS或BFS中，灌装机的生产能力为每分钟50至300瓶。考虑到制造的各种产品以及将在未来生产的产品，BBIL拥有一个开发QC实验室，该实验室参与不同试验的开发和验证，以及一个强大的QC部门，用于所有疫苗和生物治疗药物的常规批次测试和发布。
制造能力：每年大约生产5亿剂疫苗和400万单位的生物治疗药物。大约20-30％的疫苗供应给GAVI国家。
BBIL拥有强大的QC部门，生产、测试和发布下面的疫苗和生物治疗药物。除了BBIL提供的设备外，在紧急情况下，可以用班加罗尔BIOVET的BSL3/Ag+设备获得5,000L的更高制造能力。
实施例6
CORAVAX(rDNA-BBV151)
为了评估经由肌肉注射途径施用的BBV151(灭活狂犬病载体平台冠状病毒)疫苗的反应原性和安全性，将对健康志愿者进行肌肉注射狂犬病灭活载体平台冠状病毒疫苗(rDNA-BBV151)的剂量递增研究。
CORAVAX(rDNA-BBV151)是一种有佐剂的狂犬病载体冠状病毒疫苗，它表达与RABV糖蛋白(G)的N末端结构域部分融合的SARSCoV-2刺突(S)蛋白的S1结构域，并整合到RABV中颗粒中。CORAVAX(rDNA-BBV151)疫苗有两种剂型(presentation)。
Figure GDA0004183787440002031
Figure GDA0004183787440002041
剂型1(BBV151-A)–成分I和II将作为两个单独的小瓶(每个0.5mL)提供，这两个小瓶(A和B)将在施用时混合。最终重构的1.0mL体积相当于2剂。BBV151-A剂型有两种疫苗制剂，其活性成分的剂量强度有所不同(图XA)：
BBV151-A1：BBV151-A1制剂具有15mcg活性成分。
BBV151-A2：BBV151-A2制剂具有30mcg活性成分。
剂型2(BBV151-B)：成分I和II将在其之前混合在一起，并将作为单个小瓶(0.5mL剂量体积)提供。BBV151-B剂型只有一种制剂(图XB)：
BBV151-B：BBV151-B制剂具有30mcg活性成分。
该研究旨在评估接受疫苗或安慰剂的四组健康志愿者的安全性、反应原性和免疫原性。
第1组(BBV151-A1)：在该组中，将招募15名参与者，并在第0天和第28天经由肌肉注射途径施用BBV151-A1疫苗制剂。
第2组(BBV151-A2)：在该组中，将招募15名参与者，并在第0天和第28天经由肌肉注射途径施用BBV151-A2疫苗制剂。
第3组(BBV151-B)：在该组中，将招募15名参与者，并在第0天经由肌肉注射途径施用BBV151-B疫苗制剂。
数据将对第三方生物统计学家不设盲，并将在第42天进行免疫原性和安全性的中期分析。COVID-19病毒的nAb滴度将通过MNT/PRNT测定进行评估，并根据疫苗与安慰剂组比较的GMT评估免疫原性。将评估血清样本的nAb。将使用ELISA评估血清样本中针对SARS-CoV-2病毒刺突蛋白的结合抗体滴度。将从研究人群的子集中评估细胞介导的免疫。
将分别计算每个疫苗组(一剂和两剂)中的中和滴度的GMT。GMT的两侧95％置信区间(CI)将从log10-转换滴度均值的95％ CI计算，使用log10(滴度)分布的正态近似。还将展示两个疫苗组(两剂GMT/一剂GMT)和相应的95％ CI。GMT比率的两侧95％ CI将从log10(滴度)均值差异的95％ CI计算。两个疫苗组将在5％显著性水平上使用log10-转换滴度均值的两侧双样本t检验进行比较。
为了评估BBV151的体液免疫反应，将通过MNT/PRNT测定在三组中，从基线到第28±2、42±2、90±7天和180±7天进行GMT和中和抗体(NAb)的四倍血清转化率(SCR)。
为了比较单剂量组和双剂量组之间的体液反应，将通过MNT/PRNT测定在三组中，从基线到第28+2天、42±2、90±7和180±7天的GMT和中和抗体(NAb)的四倍血清转化率(SCR)。
为了评估对SARS-CoV-2病毒的刺突蛋白和狂犬病载体的免疫反应，将确定三组中从基线到第28+2、42±2、90±7和180±7天的GMT和针对刺突蛋白的结合抗体(bAb's)IgA和IgG的四倍血清转化率。将通过ELISA从基线到第28+2、42±2、90±7和180±7天评估对载体的免疫反应(结合/或中和)。
为了在评估特别关注的不良事件(AESI)方面评估疫苗的安全性，将在整个研究期间监测和记录AESI的发生。
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其他实施方式
此处对变量的任何定义中的要素列表的引用包括将该变量定义为任何单个要素或所列要素的组合(或子组合)。本文对实施方式的叙述包括作为任何单个实施方式或与任何其他实施方式或其部分组合的实施方式。
本文引用的每篇专利、专利申请和出版物的公开内容均通过引用以其整体并入本文。虽然本发明已经参照具体实施方式进行了公开，但是显然本领域的其他技术人员可以设计出本发明的其他实施方式和变化而不背离本发明的真实精神和范围。所附权利要求旨在被解释为包括所有这样的实施方式和等效变型。
序列表
<110> 托马斯杰斐逊大学
<120> 冠状病毒疾病(COVID-19)疫苗
<130> 205961-7055WO1(00200)
<150> 62/986,396
<151> 2020-03-06
<150> 63/017,241
<151> 2020-04-29
<160> 106
<170> PatentIn version 3.5
<210> 1
<211> 17189
<212> DNA
<213> 人工序列
<220>
<223> VSV-COVID19-S1-VSVG
<400> 1
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ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 180
ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 240
ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 300
gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 360
tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 420
ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg 480
caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 540
gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 600
taaaacgacg gccagtgagc gcgccctagt tattaatagt aatcaattac ggggtcatta 660
gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc 720
tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg 780
ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg 840
gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa 900
tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac 960
atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 1020
cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 1080
agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 1140
ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg 1200
ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag 1260
acccaagctg gctagggtct tcgtctgatg agtccgtgag gacgaaaccc ggcgtaccgg 1320
gtcacgaaga caaacaaacc attattatca ttaaaaggct caggagaaac tttaacagta 1380
atcaaaatgt ctgttacagt caagagaatc attgacaaca cagtcatagt tccaaaactt 1440
cctgcaaatg aggatccagt ggaatacccg gcagattact tcagaaaatc aaaggagatt 1500
cctctttaca tcaatactac aaaaagtttg tcagatctaa gaggatatgt ctaccaaggc 1560
ctcaaatccg gaaatgtatc aatcatacat gtcaacagct acttgtatgg agcattaaag 1620
gacatccggg gtaagttgga taaagattgg tcaagtttcg gaataaacat cgggaaagca 1680
ggggatacaa tcggaatatt tgaccttgta tccttgaaag ccctggacgg cgtacttcca 1740
gatggagtat cggatgcttc cagaaccagc gcagatgaca aatggttgcc tttgtatcta 1800
cttggcttat acagagtggg cagaacacaa atgcctgaat acagaaaaaa gctcatggat 1860
gggctgacaa atcaatgcaa aatgatcaat gaacagtttg aacctcttgt gccagaaggt 1920
cgtgacattt ttgatgtgtg gggaaatgac agtaattaca caaaaattgt cgctgcagtg 1980
gacatgttct tccacatgtt caaaaaacat gaatgtgcct cgttcagata cggaactatt 2040
gtttccagat tcaaagattg tgctgcattg gcaacatttg gacacctctg caaaataacc 2100
ggaatgtcta cagaagatgt aacgacctgg atcttgaacc gagaagttgc agatgaaatg 2160
gtccaaatga tgcttccagg ccaagaaatt gacaaggccg attcatacat gccttatttg 2220
atcgactttg gattgtcttc taagtctcca tattcttccg tcaaaaaccc tgccttccac 2280
ttctgggggc aattgacagc tcttctgctc agatccacca gagcaaggaa tgcccgacag 2340
cctgatgaca ttgagtatac atctcttact acagcaggtt tgttgtacgc ttatgcagta 2400
ggatcctctg ccgacttggc acaacagttt tgtgttggag ataacaaata cactccagat 2460
gatagtaccg gaggattgac gactaatgca ccgccacaag gcagagatgt ggtcgaatgg 2520
ctcggatggt ttgaagatca aaacagaaaa ccgactcctg atatgatgca gtatgcgaaa 2580
agagcagtca tgtcactgca aggcctaaga gagaagacaa ttggcaagta tgctaagtca 2640
gaatttgaca aatgacccta taattctcag atcacctatt atatattatg ctacatatga 2700
aaaaaactaa cagatatcat ggataatctc acaaaagttc gtgagtatct caagtcctat 2760
tctcgtctgg atcaggcggt aggagagata gatgagatcg aagcacaacg agctgaaaag 2820
tccaattatg agttgttcca agaggatgga gtggaagagc atactaagcc ctcttatttt 2880
caggcagcag atgattctga cacagaatct gaaccagaaa ttgaagacaa tcaaggtttg 2940
tatgcaccag atccagaagc tgagcaagtt gaaggcttta tacaggggcc tttagatgac 3000
tatgcagatg aggaagtgga tgttgtattt acttcggact ggaaacagcc tgagcttgaa 3060
tctgacgagc atggaaagac cttacggttg acatcgccag agggtttaag tggagagcag 3120
aaatcccagt ggctttcgac gattaaagca gtcgtgcaaa gtgccaaata ctggaatctg 3180
gcagagtgca catttgaagc atcgggagaa ggggtcatta tgaaggagcg ccagataact 3240
ccggatgtat ataaggtcac tccagtgatg aacacacatc cgtcccaatc agaagcagta 3300
tcagatgttt ggtctctctc aaagacatcc atgactttcc aacccaagaa agcaagtctt 3360
cagcctctca ccatatcctt ggatgaattg ttctcatcta gaggagagtt catctctgtc 3420
ggaggtgacg gacgaatgtc tcataaagag gccatcctgc tcggcctgag atacaaaaag 3480
ttgtacaatc aggcgagagt caaatattct ctgtagacta tgaaaaaaag taacagatat 3540
cacgatctaa gtgttatccc aatccattca tcatgagttc cttaaagaag attctcggtc 3600
tgaaggggaa aggtaagaaa tctaagaaat tagggatcgc accaccccct tatgaagagg 3660
acactagcat ggagtatgct ccgagcgctc caattgacaa atcctatttt ggagttgacg 3720
agatggacac ctatgatccg aatcaattaa gatatgagaa attcttcttt acagtgaaaa 3780
tgacggttag atctaatcgt ccgttcagaa catactcaga tgtggcagcc gctgtatccc 3840
attgggatca catgtacatc ggaatggcag ggaaacgtcc cttctacaaa atcttggctt 3900
ttttgggttc ttctaatcta aaggccactc cagcggtatt ggcagatcaa ggtcaaccag 3960
agtatcacac tcactgcgaa ggcagggctt atttgccaca taggatgggg aagacccctc 4020
ccatgctcaa tgtaccagag cacttcagaa gaccattcaa tataggtctt tacaagggaa 4080
cgattgagct cacaatgacc atctacgatg atgagtcact ggaagcagct cctatgatct 4140
gggatcattt caattcttcc aaattttctg atttcagaga gaaggcctta atgtttggcc 4200
tgattgtcga gaaaaaggca tctggagcgt gggtcctgga ttctatcagc cacttcaaat 4260
gagctagtct aacttctagc ttctgaacaa tccccggttt actcagtctc tcctaattcc 4320
agcctctcga acaactaata tcctgtcttt tctatcccta tgaaaaaaac taacagagat 4380
cgatctgttt acgcgtcact atgaagtgcc ttttgtactt agccttttta ttcattgggg 4440
tgaattgcaa gttcaccata gtttttccac acaaccaaaa aggaaactgg aaaaatgttc 4500
cttctaatta ccattattgc ccgtcaagct cagatttaaa ttggcataat gacttaatag 4560
gcacagccat acaagtcaaa atgcccaaga gtcacaaggc tattcaagca gacggttgga 4620
tgtgtcatgc ttccaaatgg gtcactactt gtgatttccg ctggtatgga ccgaagtata 4680
taacacagtc catccgatcc ttcactccat ctgtagaaca atgcaaggaa agcattgaac 4740
aaacgaaaca aggaacttgg ctgaatccag gcttccctcc tcaaagttgt ggatatgcaa 4800
ctgtgacgga tgccgaagca gtgattgtcc aggtgactcc tcaccatgtg ctggttgatg 4860
aatacacagg agaatgggtt gattcacagt tcatcaacgg aaaatgcagc aattacatat 4920
gccccactgt ccataactct acaacctggc attctgacta taaggtcaaa gggctatgtg 4980
attctaacct catttccatg gacatcacct tcttctcaga ggacggagag ctatcatccc 5040
tgggaaagga gggcacaggg ttcagaagta actactttgc ttatgaaact ggaggcaagg 5100
cctgcaaaat gcaatactgc aagcattggg gagtcagact cccatcaggt gtctggttcg 5160
agatggctga taaggatctc tttgctgcag ccagattccc tgaatgccca gaagggtcaa 5220
gtatctctgc tccatctcag acctcagtgg atgtaagtct aattcaggac gttgagagga 5280
tcttggatta ttccctctgc caagaaacct ggagcaaaat cagagcgggt cttccaatct 5340
ctccagtgga tctcagctat cttgctccta aaaacccagg aaccggtcct gctttcacca 5400
taatcaatgg taccctaaaa tactttgaga ccagatacat cagagtcgat attgctgctc 5460
caatcctctc aagaatggtc ggaatgatca gtggaactac cacagaaagg gaactgtggg 5520
atgactgggc accatatgaa gacgtggaaa ttggacccaa tggagttctg aggaccagtt 5580
caggatataa gtttccttta tacatgattg gacatggtat gttggactcc gatcttcatc 5640
ttagctcaaa ggctcaggtg ttcgaacatc ctcacattca agacgctgct tcgcaacttc 5700
ctgatgatga gagtttattt tttggtgata ctgggctatc caaaaatcca atcgagcttg 5760
tagaaggttg gttcagtagt tggaaaagct ctattgcctc ttttttcttt atcatagggt 5820
taatcattgg actattcttg gttctccgag ttggtatcca tctttgcatt aaattaaagc 5880
acaccaagaa aagacagatt tatacagaca tagagatgaa ccgacttgga aagtaactca 5940
aatcctgcta ggtatgaaaa aaactaacag atatcacgct cgagcgtacg ccaccatgtt 6000
cgtgtttctg gtgctgctgc ctctggtgag ctcccagtgc gtgaacctga ccacaaggac 6060
ccagctgccc cctgcctata ccaattcctt cacacggggc gtgtactatc ccgacaaggt 6120
gttccggagc agcgtgctgc actccacaca ggatctgttt ctgcctttct tttctaacgt 6180
gacctggttc cacgccatcc acgtgagcgg caccaatggc acaaagcggt tcgacaatcc 6240
agtgctgccc tttaacgatg gcgtgtactt cgcctccacc gagaagtcta acatcatcag 6300
aggctggatc tttggcacca cactggacag caagacacag tccctgctga tcgtgaacaa 6360
tgccaccaac gtggtcatca aggtgtgcga gttccagttt tgtaatgatc cattcctggg 6420
cgtgtactat cacaagaaca ataagtcttg gatggagagc gagtttcgcg tgtattcctc 6480
tgccaacaat tgcacatttg agtacgtgtc ccagcccttc ctgatggacc tggagggcaa 6540
gcagggcaat ttcaagaacc tgagggagtt cgtgtttaag aatatcgatg gctacttcaa 6600
aatctactcc aagcacaccc caatcaacct ggtgcgcgac ctgccacagg gcttctctgc 6660
cctggagcca ctggtggatc tgcccatcgg catcaacatc acccggtttc agacactgct 6720
ggccctgcac agaagctacc tgacaccagg cgacagctcc tctggatgga ccgcaggagc 6780
agcagcctac tatgtgggct atctgcagcc caggaccttc ctgctgaagt acaacgagaa 6840
tggcaccatc acagacgccg tggattgcgc cctggatccc ctgtctgaga ccaagtgtac 6900
actgaagagc tttaccgtgg agaagggcat ctatcagaca agcaatttca gggtgcagcc 6960
taccgagtcc atcgtgcgct ttcccaatat cacaaacctg tgcccttttg gcgaggtgtt 7020
caacgcaacc cgcttcgcca gcgtgtacgc ctggaatagg aagcgcatct ccaactgcgt 7080
ggccgactat tctgtgctgt acaacagcgc ctccttctct acctttaagt gctatggcgt 7140
gagccccaca aagctgaatg acctgtgctt taccaacgtg tacgccgatt ccttcgtgat 7200
caggggcgac gaggtgcgcc agatcgcacc aggacagaca ggcaagatcg cagactacaa 7260
ttataagctg cctgacgatt tcaccggctg cgtgatcgcc tggaactcta acaatctgga 7320
tagcaaagtg ggcggcaact acaattatct gtaccggctg tttagaaagt ctaatctgaa 7380
gccattcgag agggacatct ccacagaaat ctaccaggcc ggctctaccc cctgcaatgg 7440
cgtggagggc tttaactgtt atttccctct gcagagctac ggcttccagc caacaaacgg 7500
cgtgggctat cagccctacc gcgtggtggt gctgtctttt gagctgctgc acgcacctgc 7560
aacagtgtgc ggaccaaaga agagcaccaa tctggtgaag aacaagtgcg tgaacttcaa 7620
cttcaacgga ctgaccggaa caggcgtgct gaccgagtcc aacaagaagt tcctgccttt 7680
tcagcagttc ggcagggaca tcgcagatac cacagacgcc gtgcgcgacc ctcagaccct 7740
ggagatcctg gacatcacac catgctcctt cggcggcgtg tctgtgatca caccaggcac 7800
caatacaagc aaccaggtgg ccgtgctgta tcaggacgtg aattgtaccg aggtgccagt 7860
ggcaatccac gcagatcagc tgacccctac atggcgggtg tactctaccg gcagcaacgt 7920
gttccagaca agagccggat gcctgatcgg agcagagcac gtgaacaata gctatgagtg 7980
cgacatccct atcggcgccg gcatctgtgc ctcctaccag acccagacaa actccccaag 8040
gtctgtgggc gatacaggcc tgtccaagaa tccaatcgag ctggtagagg gctggttcag 8100
cagttggaaa agctccatcg cctccttttt ctttatcatc ggcctgatca tcggactgtt 8160
cctggtgctc cgcgtgggta tccacctgtg catcaagctg aagcacacca agaaaagaca 8220
gatttataca gacatcgaga tgaaccgcct gggaaagtga gctagccaga ttcttcatgt 8280
ttggaccaaa tcaacttgtg ataccatgct caaagaggcc tcaattatat ttgagttttt 8340
aatttttatg aaaaaaacta acagcaatca tggaagtcca cgattttgag accgacgagt 8400
tcaatgattt caatgaagat gactatgcca caagagaatt cctgaatccc gatgagcgca 8460
tgacgtactt gaatcatgct gattacaatt tgaattctcc tctaattagt gatgatattg 8520
acaatttgat caggaaattc aattctcttc cgattccctc gatgtgggat agtaagaact 8580
gggatggagt tcttgagatg ttaacatcat gtcaagccaa tcccatctca acatctcaga 8640
tgcataaatg gatgggaagt tggttaatgt ctgataatca tgatgccagt caagggtata 8700
gttttttaca tgaagtggac aaagaggcag aaataacatt tgacgtggtg gagaccttca 8760
tccgcggctg gggcaacaaa ccaattgaat acatcaaaaa ggaaagatgg actgactcat 8820
tcaaaattct cgcttatttg tgtcaaaagt ttttggactt acacaagttg acattaatct 8880
taaatgctgt ctctgaggtg gaattgctca acttggcgag gactttcaaa ggcaaagtca 8940
gaagaagttc tcatggaacg aacatatgca ggattagggt tcccagcttg ggtcctactt 9000
ttatttcaga aggatgggct tacttcaaga aacttgatat tctaatggac cgaaactttc 9060
tgttaatggt caaagatgtg attataggga ggatgcaaac ggtgctatcc atggtatgta 9120
gaatagacaa cctgttctca gagcaagaca tcttctccct tctaaatatc tacagaattg 9180
gagataaaat tgtggagagg cagggaaatt tttcttatga cttgattaaa atggtggaac 9240
cgatatgcaa cttgaagctg atgaaattag caagagaatc aaggccttta gtcccacaat 9300
tccctcattt tgaaaatcat atcaagactt ctgttgatga aggggcaaaa attgaccgag 9360
gtataagatt cctccatgat cagataatga gtgtgaaaac agtggatctc acactggtga 9420
tttatggatc gttcagacat tggggtcatc cttttataga ttattacact ggactagaaa 9480
aattacattc ccaagtaacc atgaagaaag atattgatgt gtcatatgca aaagcacttg 9540
caagtgattt agctcggatt gttctatttc aacagttcaa tgatcataaa aagtggttcg 9600
tgaatggaga cttgctccct catgatcatc cctttaaaag tcatgttaaa gaaaatacat 9660
ggcccacagc tgctcaagtt caagattttg gagataaatg gcatgaactt ccgctgatta 9720
aatgttttga aatacccgac ttactagacc catcgataat atactctgac aaaagtcatt 9780
caatgaatag gtcagaggtg ttgaaacatg tccgaatgaa tccgaacact cctatcccta 9840
gtaaaaaggt gttgcagact atgttggaca caaaggctac caattggaaa gaatttctta 9900
aagagattga tgagaagggc ttagatgatg atgatctaat tattggtctt aaaggaaagg 9960
agagggaact gaagttggca ggtagatttt tctccctaat gtcttggaaa ttgcgagaat 10020
actttgtaat taccgaatat ttgataaaga ctcatttcgt ccctatgttt aaaggcctga 10080
caatggcgga cgatctaact gcagtcatta aaaagatgtt agattcctca tccggccaag 10140
gattgaagtc atatgaggca atttgcatag ccaatcacat tgattacgaa aaatggaata 10200
accaccaaag gaagttatca aacggcccag tgttccgagt tatgggccag ttcttaggtt 10260
atccatcctt aatcgagaga actcatgaat tttttgagaa aagtcttata tactacaatg 10320
gaagaccaga cttgatgcgt gttcacaaca acacactgat caattcaacc tcccaacgag 10380
tttgttggca aggacaagag ggtggactgg aaggtctacg gcaaaaagga tggactatcc 10440
tcaatctact ggttattcaa agagaggcta aaatcagaaa cactgctgtc aaagtcttgg 10500
cacaaggtga taatcaagtt atttgcacac agtataaaac gaagaaatcg agaaacgttg 10560
tagaattaca gggtgctctc aatcaaatgg tttctaataa tgagaaaatt atgactgcaa 10620
tcaaaatagg gacagggaag ttaggacttt tgataaatga cgatgagact atgcaatctg 10680
cagattactt gaattatgga aaaataccga ttttccgtgg agtgattaga gggttagaga 10740
ccaagagatg gtcacgagtg acttgtgtca ccaatgacca aatacccact tgtgctaata 10800
taatgagctc agtttccaca aatgctctca ccgtagctca ttttgctgag aacccaatca 10860
atgccatgat acagtacaat tattttggga catttgctag actcttgttg atgatgcatg 10920
atcctgctct tcgtcaatca ttgtatgaag ttcaagataa gataccgggc ttgcacagtt 10980
ctactttcaa atacgccatg ttgtatttgg acccttccat tggaggagtg tcgggcatgt 11040
ctttgtccag gtttttgatt agagccttcc cagatcccgt aacagaaagt ctctcattct 11100
ggagattcat ccatgtacat gctcgaagtg agcatctgaa ggagatgagt gcagtatttg 11160
gaaaccccga gatagccaag tttcgaataa ctcacataga caagctagta gaagatccaa 11220
cctctctgaa catcgctatg ggaatgagtc cagcgaactt gttaaagact gaggttaaaa 11280
aatgcttaat cgaatcaaga caaaccatca ggaaccaggt gattaaggat gcaaccatat 11340
atttgtatca tgaagaggat cggctcagaa gtttcttatg gtcaataaat cctctgttcc 11400
ctagattttt aagtgaattc aaatcaggca cttttttggg agtcgcagac gggctcatca 11460
gtctatttca aaattctcgt actattcgga actcctttaa gaaaaagtat catagggaat 11520
tggatgattt gattgtgagg agtgaggtat cctctttgac acatttaggg aaacttcatt 11580
tgagaagggg atcatgtaaa atgtggacat gttcagctac tcatgctgac acattaagat 11640
acaaatcctg gggccgtaca gttattggga caactgtacc ccatccatta gaaatgttgg 11700
gtccacaaca tcgaaaagag actccttgtg caccatgtaa cacatcaggg ttcaattatg 11760
tttctgtgca ttgtccagac gggatccatg acgtctttag ttcacgggga ccattgcctg 11820
cttatctagg gtctaaaaca tctgaatcta catctatttt gcagccttgg gaaagggaaa 11880
gcaaagtccc actgattaaa agagctacac gtcttagaga tgctatctct tggtttgttg 11940
aacccgactc taaactagca atgactatac tttctaacat ccactcttta acaggcgaag 12000
aatggaccaa aaggcagcat gggttcaaaa gaacagggtc tgcccttcat aggttttcga 12060
catctcggat gagccatggt gggttcgcat ctcagagcac tgcagcattg accaggttga 12120
tggcaactac agacaccatg agggatctgg gagatcagaa tttcgacttt ttattccaag 12180
caacgttgct ctatgctcaa attaccacca ctgttgcaag agacggatgg atcaccagtt 12240
gtacagatca ttatcatatt gcctgtaagt cctgtttgag acccatagaa gagatcaccc 12300
tggactcaag tatggactac acgcccccag atgtatccca tgtgctgaag acatggagga 12360
atggggaagg ttcgtgggga caagagataa aacagatcta tcctttagaa gggaattgga 12420
agaatttagc acctgctgag caatcctatc aagtcggcag atgtataggt tttctatatg 12480
gagacttggc gtatagaaaa tctactcatg ccgaggacag ttctctattt cctctatcta 12540
tacaaggtcg tattagaggt cgaggtttct taaaagggtt gctagacgga ttaatgagag 12600
caagttgctg ccaagtaata caccggagaa gtctggctca tttgaagagg ccggccaacg 12660
cagtgtacgg aggtttgatt tacttgattg ataaattgag tgtatcacct ccattccttt 12720
ctcttactag atcaggacct attagagacg aattagaaac gattccccac aagatcccaa 12780
cctcctatcc gacaagcaac cgtgatatgg gggtgattgt cagaaattac ttcaaatacc 12840
aatgccgtct aattgaaaag ggaaaataca gatcacatta ttcacaatta tggttattct 12900
cagatgtctt atccatagac ttcattggac cattctctat ttccaccacc ctcttgcaaa 12960
tcctatacaa gccattttta tctgggaaag ataagaatga gttgagagag ctggcaaatc 13020
tttcttcatt gctaagatca ggagaggggt gggaagacat acatgtgaaa ttcttcacca 13080
aggacatatt attgtgtcca gaggaaatca gacatgcttg caagttcggg attgctaagg 13140
ataataataa agacatgagc tatccccctt ggggaaggga atccagaggg acaattacaa 13200
caatccctgt ttattatacg accacccctt acccaaagat gctagagatg cctccaagaa 13260
tccaaaatcc cctgctgtcc ggaatcaggt tgggccaatt accaactggc gctcattata 13320
aaattcggag tatattacat ggaatgggaa tccattacag ggacttcttg agttgtggag 13380
acggctccgg agggatgact gctgcattac tacgagaaaa tgtgcatagc agaggaatat 13440
tcaatagtct gttagaatta tcagggtcag tcatgcgagg cgcctctcct gagcccccca 13500
gtgccctaga aactttagga ggagataaat cgagatgtgt aaatggtgaa acatgttggg 13560
aatatccatc tgacttatgt gacccaagga cttgggacta tttcctccga ctcaaagcag 13620
gcttggggct tcaaattgat ttaattgtaa tggatatgga agttcgggat tcttctacta 13680
gcctgaaaat tgagacgaat gttagaaatt atgtgcaccg gattttggat gagcaaggag 13740
ttttaatcta caagacttat ggaacatata tttgtgagag cgaaaagaat gcagtaacaa 13800
tccttggtcc catgttcaag acggtcgact tagttcaaac agaatttagt agttctcaaa 13860
cgtctgaagt atatatggta tgtaaaggtt tgaagaaatt aatcgatgaa cccaatcccg 13920
attggtcttc catcaatgaa tcctggaaaa acctgtacgc attccagtca tcagaacagg 13980
aatttgccag agcaaagaag gttagtacat actttacctt gacaggtatt ccctcccaat 14040
tcattcctga tccttttgta aacattgaga ctatgctaca aatattcgga gtacccacgg 14100
gtgtgtctca tgcggctgcc ttaaaatcat ctgatagacc tgcagattta ttgaccatta 14160
gcctttttta tatggcgatt atatcgtatt ataacatcaa tcatatcaga gtaggaccga 14220
tacctccgaa ccccccatca gatggaattg cacaaaatgt ggggatcgct ataactggta 14280
taagcttttg gctgagtttg atggagaaag acattccact atatcaacag tgtttagcag 14340
ttatccagca atcattcccg attaggtggg aggctgtttc agtaaaagga ggatacaagc 14400
agaagtggag tactagaggt gatgggctcc caaaagatac ccgaacttca gactccttgg 14460
ccccaatcgg gaactggatc agatctctgg aattggtccg aaaccaagtt cgtctaaatc 14520
cattcaatga gatcttgttc aatcagctat gtcgtacagt ggataatcat ttgaaatggt 14580
caaatttgcg aagaaacaca ggaatgattg aatggatcaa tagacgaatt tcaaaagaag 14640
accggtctat actgatgttg aagagtgacc tacacgagga aaactcttgg agagattaaa 14700
aaatcatgag gagactccaa actttaagta tgaaaaaaac tttgatcctt aagaccctct 14760
tgtggttttt attttttatc tggttttgtg gtcttcgtgg gtcggcatgg catctccacc 14820
tcctcgcggt ccgacctggg catccgaagg aggacgcacg tccactcgga tggctaaggg 14880
agagccagaa aataactagt ggatccggct gctaacaaag cccgaaagga agctgagttg 14940
gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg 15000
aggggttttt tgctgaaagt cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg 15060
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 15120
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 15180
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 15240
cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 15300
tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 15360
aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 15420
aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 15480
tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 15540
ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 15600
cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 15660
ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 15720
ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 15780
gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 15840
agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 15900
cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 15960
aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 16020
aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 16080
ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 16140
aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 16200
ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 16260
agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 16320
cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 16380
ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 16440
gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 16500
cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 16560
cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 16620
ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 16680
catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 16740
tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 16800
ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 16860
catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 16920
cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 16980
cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 17040
acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 17100
ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 17160
tccgcgcaca tttccccgaa aagtgccac 17189
<210> 2
<211> 17611
<212> DNA
<213> 人工序列
<220>
<223> BNSP333-COVID19-S1-RVG
<400> 2
ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 60
ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 120
ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 180
ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 240
ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 300
gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 360
tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 420
ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg 480
caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 540
gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 600
taaaacgacg gccagtgagc gcgccctagt tattaatagt aatcaattac ggggtcatta 660
gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc 720
tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg 780
ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg 840
gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa 900
tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac 960
atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 1020
cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 1080
agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 1140
ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg 1200
ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag 1260
acccaagctg gctagattaa gcgtctgatg agtccgtgag gacgaaaccc ggcgtaccgg 1320
gtcacgctta acaaccagat caaagaaaaa acagacattg tcaattgcaa agcaaaaatg 1380
taacacccct acaatggatg ccgacaagat tgtattcaaa gtcaataatc aggtggtctc 1440
tttgaagcct gagattatcg tggatcaata tgagtacaag taccctgcca tcaaagattt 1500
gaaaaagccc tgtataaccc taggaaaggc tcccgattta aataaagcat acaagtcagt 1560
tttgtcaggc atgagcgccg ccaaacttaa tcctgacgat gtatgttcct atttggcagc 1620
ggcaatgcag ttttttgagg ggacatgtcc ggaagactgg accagctatg gaattgtgat 1680
tgcacgaaaa ggagataaga tcaccccagg ttctctggtg gagataaaac gtactgatgt 1740
agaagggaat tgggctctga caggaggcat ggaactgaca agagacccca ctgtccctga 1800
gcatgcgtcc ttagtcggtc ttctcttgag tctgtatagg ttgagcaaaa tatccgggca 1860
aaacactggt aactataaga caaacattgc agacaggata gagcagattt ttgagacagc 1920
cccttttgtt aaaatcgtgg aacaccatac tctaatgaca actcacaaaa tgtgtgctaa 1980
ttggagtact ataccaaact tcagattttt ggccggaacc tatgacatgt ttttctcccg 2040
gattgagcat ctatattcag caatcagagt gggcacagtt gtcactgctt atgaagactg 2100
ttcaggactg gtatcattta ctgggttcat aaaacaaatc aatctcaccg ctagagaggc 2160
aatactatat ttcttccaca agaactttga ggaagagata agaagaatgt ttgagccagg 2220
gcaggagaca gctgttcctc actcttattt catccacttc cgttcactag gcttgagtgg 2280
gaaatctcct tattcatcaa atgctgttgg tcacgtgttc aatctcattc actttgtagg 2340
atgctatatg ggtcaagtca gatccctaaa tgcaacggtt attgctgcat gtgctcctca 2400
tgaaatgtct gttctagggg gctatctggg agaggaattc ttcgggaaag ggacatttga 2460
aagaagattc ttcagagatg agaaagaact tcaagaatac gaggcggctg aactgacaaa 2520
gactgacgta gcactggcag atgatggaac tgtcaactct gacgacgagg actacttttc 2580
aggtgaaacc agaagtccgg aggctgttta tactcgaatc atgatgaatg gaggtcgact 2640
aaagagatct cacatacgga gatatgtctc agtcagttcc aatcatcaag cccgtccaaa 2700
ctcattcgcc gagtttctaa acaagacata ttcgagtgac tcataacatg aaaaaaacta 2760
acacccctcc cgtacgccac catgttcgtg tttctggtgc tgctgcctct ggtgagctcc 2820
cagtgcgtga acctgaccac aaggacccag ctgccccctg cctataccaa ttccttcaca 2880
cggggcgtgt actatcccga caaggtgttc cggagcagcg tgctgcactc cacacaggat 2940
ctgtttctgc ctttcttttc taacgtgacc tggttccacg ccatccacgt gagcggcacc 3000
aatggcacaa agcggttcga caatccagtg ctgcccttta acgatggcgt gtacttcgcc 3060
tccaccgaga agtctaacat catcagaggc tggatctttg gcaccacact ggacagcaag 3120
acacagtccc tgctgatcgt gaacaatgcc accaacgtgg tcatcaaggt gtgcgagttc 3180
cagttttgta atgatccatt cctgggcgtg tactatcaca agaacaataa gtcttggatg 3240
gagagcgagt ttcgcgtgta ttcctctgcc aacaattgca catttgagta cgtgtcccag 3300
cccttcctga tggacctgga gggcaagcag ggcaatttca agaacctgag ggagttcgtg 3360
tttaagaata tcgatggcta cttcaaaatc tactccaagc acaccccaat caacctggtg 3420
cgcgacctgc cacagggctt ctctgccctg gagccactgg tggatctgcc catcggcatc 3480
aacatcaccc ggtttcagac actgctggcc ctgcacagaa gctacctgac accaggcgac 3540
agctcctctg gatggaccgc aggagcagca gcctactatg tgggctatct gcagcccagg 3600
accttcctgc tgaagtacaa cgagaatggc accatcacag acgccgtgga ttgcgccctg 3660
gatcccctgt ctgagaccaa gtgtacactg aagagcttta ccgtggagaa gggcatctat 3720
cagacaagca atttcagggt gcagcctacc gagtccatcg tgcgctttcc caatatcaca 3780
aacctgtgcc cttttggcga ggtgttcaac gcaacccgct tcgccagcgt gtacgcctgg 3840
aataggaagc gcatctccaa ctgcgtggcc gactattctg tgctgtacaa cagcgcctcc 3900
ttctctacct ttaagtgcta tggcgtgagc cccacaaagc tgaatgacct gtgctttacc 3960
aacgtgtacg ccgattcctt cgtgatcagg ggcgacgagg tgcgccagat cgcaccagga 4020
cagacaggca agatcgcaga ctacaattat aagctgcctg acgatttcac cggctgcgtg 4080
atcgcctgga actctaacaa tctggatagc aaagtgggcg gcaactacaa ttatctgtac 4140
cggctgttta gaaagtctaa tctgaagcca ttcgagaggg acatctccac agaaatctac 4200
caggccggct ctaccccctg caatggcgtg gagggcttta actgttattt ccctctgcag 4260
agctacggct tccagccaac aaacggcgtg ggctatcagc cctaccgcgt ggtggtgctg 4320
tcttttgagc tgctgcacgc acctgcaaca gtgtgcggac caaagaagag caccaatctg 4380
gtgaagaaca agtgcgtgaa cttcaacttc aacggactga ccggaacagg cgtgctgacc 4440
gagtccaaca agaagttcct gccttttcag cagttcggca gggacatcgc agataccaca 4500
gacgccgtgc gcgaccctca gaccctggag atcctggaca tcacaccatg ctccttcggc 4560
ggcgtgtctg tgatcacacc aggcaccaat acaagcaacc aggtggccgt gctgtatcag 4620
gacgtgaatt gtaccgaggt gccagtggca atccacgcag atcagctgac ccctacatgg 4680
cgggtgtact ctaccggcag caacgtgttc cagacaagag ccggatgcct gatcggagca 4740
gagcacgtga acaatagcta tgagtgcgac atccctatcg gcgccggcat ctgtgcctcc 4800
taccagaccc agacaaactc cccaaggtct gtgggagatg aggccgaaga ctttgtggaa 4860
gtccacctgc ctgatgtgca taaccaggtg tctggcgtcg acctgggact gccaaattgg 4920
ggcaagtacg tgctgctgag tgctggagca ctgactgccc tgatgctgat cattttcctg 4980
atgacctgct gtcggcgcgt gaacagaagt gagcccactc agcacaatct gcgaggaacc 5040
gggagagaag tgtcagtcac acctcagagc gggaaaatca ttagtagttg ggaatcacat 5100
aaaagcgggg gcgagaccag gctgtgagct agccatgaaa aaaactaaca cccctccttt 5160
cgaaccatcc caaacatgag caagatcttt gtcaatccta gtgctattag agccggtctg 5220
gccgatcttg agatggctga agaaactgtt gatctgatca atagaaatat cgaagacaat 5280
caggctcatc tccaagggga acccatagag gtggacaatc tccctgagga tatggggcga 5340
cttcacctgg atgatggaaa atcgcccaac catggtgaga tagccaaggt gggagaaggc 5400
aagtatcgag aggactttca gatggatgaa ggagaggatc ctagcttcct gttccagtca 5460
tacctggaaa atgttggagt ccaaatagtc agacaaatga ggtcaggaga gagatttctc 5520
aagatatggt cacagaccgt agaagagatt atatcctatg tcgcggtcaa ctttcccaac 5580
cctccaggaa agtcttcaga ggataaatca acccagacta ctggccgaga gctcaagaag 5640
gagacaacac ccactccttc tcagagagaa agccaatcat cgaaagccag gatggcggct 5700
caaattgctt ctggccctcc agcccttgaa tggtcggcta ccaatgaaga ggatgatcta 5760
tcagtggagg ctgagatcgc tcaccagatt gcagaaagtt tctccaaaaa atataagttt 5820
ccctctcgat cctcagggat actcttgtat aattttgagc aattgaaaat gaaccttgat 5880
gatatagtta aagaggcaaa aaatgtacca ggtgtgaccc gtttagccca tgacgggtcc 5940
aaactccccc taagatgtgt actgggatgg gtcgctttgg ccaactctaa gaaattccag 6000
ttgttagtcg aatccgacaa gctgagtaaa atcatgcaag atgacttgaa tcgctataca 6060
tcttgctaac cgaacctctc ccctcagtcc ctctagacaa taaaatccga gatgtcccaa 6120
agtcaacatg aaaaaaacag gcaacaccac tgataaaatg aacctcctac gtaagatagt 6180
gaaaaaccgc agggacgagg acactcaaaa atcctctccc gcgtcagccc ctctggatga 6240
cgatgacttg tggcttccac cccctgaata cgtcccgctg aaagaactta caggcaagaa 6300
gaacatgagg aacttttgta tcaacggaag ggttaaagtg tgtagcccga atggttactc 6360
gttcaggatc ctgcggcaca ttctgaaatc attcgacgag atatattctg ggaatcatag 6420
gatgatcggg ttagtcaaag tggttattgg actggctttg tcaggatctc cagtccctga 6480
gggcctgaac tgggtataca aattgaggag aacctttatc ttccagtggg ctgattccag 6540
gggccctctt gaaggggagg agttggaata ctctcaggag atcacttggg atgatgatac 6600
tgagttcgtc ggattgcaaa taagagtgat tgcaaaacag tgtcatatcc agggcagagt 6660
ctggtgtatc aacatgaacc cgagagcatg tcaactatgg tctgacatgt ctcttcagac 6720
acaaaggtcc gaagaggaca aagattcctc tctgcttcta gaataatcag attatatccc 6780
gcaaatttat cacttgttta cctctggagg agagaacata tgggctcaac tccaaccctt 6840
gggagcaata taacaaaaaa catgttatgg tgccattaaa ccgctgcatt tcatcaaagt 6900
caagttgatt acctttacat tttgatcctc ttggatgtga aaaaaactat taacatccct 6960
caaaagaccc cgggaaagat ggttcctcag gctctcctgt ttgtacccct tctggttttt 7020
ccattgtgtt ttgggaaatt ccctatttac acgataccag acaagcttgg tccctggagt 7080
ccgattgaca tacatcacct cagctgccca aacaatttgg tagtggagga cgaaggatgc 7140
accaacctgt cagggttctc ctacatggaa cttaaagttg gatacatctt agccataaaa 7200
gtgaacgggt tcacttgcac aggcgttgtg acggaggctg aaacctacac taacttcgtt 7260
ggttatgtca caaccacgtt caaaagaaag catttccgcc caacaccaga tgcatgtaga 7320
gccgcgtaca actggaagat ggccggtgac cccagatatg aagagtctct acacaatccg 7380
taccctgact accgctggct tcgaactgta aaaaccacca aggagtctct cgttatcata 7440
tctccaagtg tggcagattt ggacccatat gacagatccc ttcactcgag ggtcttccct 7500
agcgggaagt gctcaggagt agcggtgtct tctacctact gctccactaa ccacgattac 7560
accatttgga tgcccgagaa tccgagacta gggatgtctt gtgacatttt taccaatagt 7620
agagggaaga gagcatccaa agggagtgag acttgcggct ttgtagatga aagaggccta 7680
tataagtctt taaaaggagc atgcaaactc aagttatgtg gagttctagg acttagactt 7740
atggatggaa catgggtctc gatgcaaaca tcaaatgaaa ccaaatggtg ccctcccgat 7800
aagttggtga acctgcacga ctttcgctca gacgaaattg agcaccttgt tgtagaggag 7860
ttggtcagga agagagagga gtgtctggat gcactagagt ccatcatgac aaccaagtca 7920
gtgagtttca gacgtctcag tcatttaaga aaacttgtcc ctgggtttgg aaaagcatat 7980
accatattca acaagacctt gatggaagcc gatgctcact acaagtcagt cgagacttgg 8040
aatgagatcc tcccttcaaa agggtgttta agagttgggg ggaggtgtca tcctcatgtg 8100
aacggggtgt ttttcaatgg tataatatta ggacctgacg gcaatgtctt aatcccagag 8160
atgcaatcat ccctcctcca gcaacatatg gagttgttgg aatcctcggt tatccccctt 8220
gtgcaccccc tggcagaccc gtctaccgtt ttcaaggacg gtgacgaggc tgaggatttt 8280
gttgaagttc accttcccga tgtgcacaat caggtctcag gagttgactt gggtctcccg 8340
aactggggga agtatgtatt actgagtgca ggggccctga ctgccttgat gttgataatt 8400
ttcctgatga catgttgtag aagagtcaat cgatcagaac ctacgcaaca caatctcaga 8460
gggacaggga gggaggtgtc agtcactccc caaagcggga agatcatatc ttcatgggaa 8520
tcacacaaga gtgggggtga gaccagactg taattaatta acgtcctttc aacgatccaa 8580
gtccatgaaa aaaactaaca cccctcccgt acctagctta taaagtgctg ggtcatctaa 8640
gcttttcagt cgagaaaaaa acattagatc agaagaacaa ctggcaacac ttctcaacct 8700
gagacttact tcaagatgct cgatcctgga gaggtctatg atgaccctat tgacccaatc 8760
gagttagagg ctgaacccag aggaaccccc attgtcccca acatcttgag gaactctgac 8820
tacaatctca actctccttt gatagaagat cctgctagac taatgttaga atggttaaaa 8880
acagggaata gaccttatcg gatgactcta acagacaatt gctccaggtc tttcagagtt 8940
ttgaaagatt atttcaagaa ggtagatttg ggttctctca aggtgggcgg aatggctgca 9000
cagtcaatga tttctctctg gttatatggt gcccactctg aatccaacag gagccggaga 9060
tgtataacag acttggccca tttctattcc aagtcgtccc ccatagagaa gctgttgaat 9120
ctcacgctag gaaatagagg gctgagaatc cccccagagg gagtgttaag ttgccttgag 9180
agggttgatt atgataatgc atttggaagg tatcttgcca acacgtattc ctcttacttg 9240
ttcttccatg taatcacctt atacatgaac gccctagact gggatgaaga aaagaccatc 9300
ctagcattat ggaaagattt aacctcagtg gacatcggga aggacttggt aaagttcaaa 9360
gaccaaatat ggggactgct gatcgtgaca aaggactttg tttactccca aagttccaat 9420
tgtctttttg acagaaacta cacacttatg ctaaaagatc ttttcttgtc tcgcttcaac 9480
tccttaatgg tcttgctctc tcccccagag ccccgatact cagatgactt gatatctcaa 9540
ctatgccagc tgtacattgc tggggatcaa gtcttgtcta tgtgtggaaa ctccggctat 9600
gaagtcatca aaatattgga gccatatgtc gtgaatagtt tagtccagag agcagaaaag 9660
tttaggcctc tcattcattc cttgggagac tttcctgtat ttataaaaga caaggtaagt 9720
caacttgaag agacgttcgg tccctgtgca agaaggttct ttagggctct ggatcaattc 9780
gacaacatac atgacttggt ttttgtgttt ggctgttaca ggcattgggg gcacccatat 9840
atagattatc gaaagggtct gtcaaaacta tatgatcagg ttcaccttaa aaaaatgata 9900
gataagtcct accaggagtg cttagcaagc gacctagcca ggaggatcct tagatggggt 9960
tttgataagt actccaagtg gtatctggat tcaagattcc tagcccgaga ccaccccttg 10020
actccttata tcaaaaccca aacatggcca cccaaacata ttgtagactt ggtgggggat 10080
acatggcaca agctcccgat cacgcagatc tttgagattc ctgaatcaat ggatccgtca 10140
gaaatattgg atgacaaatc acattctttc accagaacga gactagcttc ttggctgtca 10200
gaaaaccgag gggggcctgt tcctagcgaa aaagttatta tcacggccct gtctaagccg 10260
cctgtcaatc cccgagagtt tctgaggtct atagacctcg gaggattgcc agatgaagac 10320
ttgataattg gcctcaagcc aaaggaacgg gaattgaaga ttgaaggtcg attctttgct 10380
ctaatgtcat ggaatctaag attgtatttt gtcatcactg aaaaactctt ggccaactac 10440
atcttgccac tttttgacgc gctgactatg acagacaacc tgaacaaggt gtttaaaaag 10500
ctgatcgaca gggtcaccgg gcaagggctt ttggactatt caagggtcac atatgcattt 10560
cacctggact atgaaaagtg gaacaaccat caaagattag agtcaacaga ggatgtattt 10620
tctgtcctag atcaagtgtt tggattgaag agagtgtttt ctagaacaca cgagtttttt 10680
caaaaggcct ggatctatta ttcagacaga tcagacctca tcgggttacg ggaggatcaa 10740
atatactgct tagatgcgtc caacggccca acctgttgga atggccagga tggcgggcta 10800
gaaggcttac ggcagaaggg ctggagtcta gtcagcttat tgatgataga tagagaatct 10860
caaatcagga acacaagaac caaaatacta gctcaaggag acaaccaggt tttatgtccg 10920
acatacatgt tgtcgccagg gctatctcaa gaggggctcc tctatgaatt ggagagaata 10980
tcaaggaatg cactttcgat atacagagcc gtcgaggaag gggcatctaa gctagggctg 11040
atcatcaaga aagaagagac catgtgtagt tatgacttcc tcatctatgg aaaaacccct 11100
ttgtttagag gtaacatatt ggtgcctgag tccaaaagat gggccagagt ctcttgcgtc 11160
tctaatgacc aaatagtcaa cctcgccaat ataatgtcga cagtgtccac caatgcgcta 11220
acagtggcac aacactctca atctttgatc aaaccgatga gggattttct gctcatgtca 11280
gtacaggcag tctttcacta cctgctattt agcccaatct taaagggaag agtttacaag 11340
attctgagcg ctgaagggga gagctttctc ctagccatgt caaggataat ctatctagat 11400
ccttctttgg gagggatatc tggaatgtcc ctcggaagat tccatatacg acagttctca 11460
gaccctgtct ctgaagggtt atccttctgg agagagatct ggttaagctc ccaagagtcc 11520
tggattcacg cgttgtgtca agaggctgga aacccagatc ttggagagag aacactcgag 11580
agcttcactc gccttctaga agatccgacc accttaaata tcagaggagg ggccagtcct 11640
accattctac tcaaggatgc aatcagaaag gctttatatg acgaggtgga caaggtggaa 11700
aattcagagt ttcgagaggc aatcctgttg tccaagaccc atagagataa ttttatactc 11760
ttcttaatat ctgttgagcc tctgtttcct cgatttctca gtgagctatt cagttcgtct 11820
tttttgggaa tccccgagtc aatcattgga ttgatacaaa actcccgaac gataagaagg 11880
cagtttagaa agagtctctc aaaaacttta gaagaatcct tctacaactc agagatccac 11940
gggattagtc ggatgaccca gacacctcag agggttgggg gggtgtggcc ttgctcttca 12000
gagagggcag atctacttag ggagatctct tggggaagaa aagtggtagg cacgacagtt 12060
cctcaccctt ctgagatgtt gggattactt cccaagtcct ctatttcttg cacttgtgga 12120
gcaacaggag gaggcaatcc tagagtttct gtatcagtac tcccgtcctt tgatcagtca 12180
tttttttcac gaggccccct aaagggatac ttgggctcgt ccacctctat gtcgacccag 12240
ctattccatg catgggaaaa agtcactaat gttcatgtgg tgaagagagc tctatcgtta 12300
aaagaatcta taaactggtt cattactaga gattccaact tggctcaagc tctaattagg 12360
aacattatgt ctctgacagg ccctgatttc cctctagagg aggcccctgt cttcaaaagg 12420
acggggtcag ccttgcatag gttcaagtct gccagataca gcgaaggagg gtattcttct 12480
gtctgcccga acctcctctc tcatatttct gttagtacag acaccatgtc tgatttgacc 12540
caagacggga agaactacga tttcatgttc cagccattga tgctttatgc acagacatgg 12600
acatcagagc tggtacagag agacacaagg ctaagagact ctacgtttca ttggcacctc 12660
cgatgcaaca ggtgtgtgag acccattgac gacgtgaccc tggagacctc tcagatcttc 12720
gagtttccgg atgtgtcgaa aagaatatcc agaatggttt ctggggctgt gcctcacttc 12780
cagaggcttc ccgatatccg tctgagacca ggagattttg aatctctaag cggtagagaa 12840
aagtctcacc atatcggatc agctcagggg ctcttatact caatcttagt ggcaattcac 12900
gactcaggat acaatgatgg aaccatcttc cctgtcaaca tatacggcaa ggtttcccct 12960
agagactatt tgagagggct cgcaagggga gtattgatag gatcctcgat ttgcttcttg 13020
acaagaatga caaatatcaa tattaataga cctcttgaat tggtctcagg ggtaatctca 13080
tatattctcc tgaggctaga taaccatccc tccttgtaca taatgctcag agaaccgtct 13140
cttagaggag agatattttc tatccctcag aaaatccccg ccgcttatcc aaccactatg 13200
aaagaaggca acagatcaat cttgtgttat ctccaacatg tgctacgcta tgagcgagag 13260
ataatcacgg cgtctccaga gaatgactgg ctatggatct tttcagactt tagaagtgcc 13320
aaaatgacgt acctatccct cattacttac cagtctcatc ttctactcca gagggttgag 13380
agaaacctat ctaagagtat gagagataac ctgcgacaat tgagttcttt gatgaggcag 13440
gtgctgggcg ggcacggaga agatacctta gagtcagacg acaacattca acgactgcta 13500
aaagactctt tacgaaggac aagatgggtg gatcaagagg tgcgccatgc agctagaacc 13560
atgactggag attacagccc caacaagaag gtgtcccgta aggtaggatg ttcagaatgg 13620
gtctgctctg ctcaacaggt tgcagtctct acctcagcaa acccggcccc tgtctcggag 13680
cttgacataa gggccctctc taagaggttc cagaaccctt tgatctcggg cttgagagtg 13740
gttcagtggg caaccggtgc tcattataag cttaagccta ttctagatga tctcaatgtt 13800
ttcccatctc tctgccttgt agttggggac gggtcagggg ggatatcaag ggcagtcctc 13860
aacatgtttc cagatgccaa gcttgtgttc aacagtcttt tagaggtgaa tgacctgatg 13920
gcttccggaa cacatccact gcctccttca gcaatcatga ggggaggaaa tgatatcgtc 13980
tccagagtga tagatcttga ctcaatctgg gaaaaaccgt ccgacttgag aaacttggca 14040
acctggaaat acttccagtc agtccaaaag caggtcaaca tgtcctatga cctcattatt 14100
tgcgatgcag aagttactga cattgcatct atcaaccgga tcaccctgtt aatgtccgat 14160
tttgcattgt ctatagatgg accactctat ttggtcttca aaacttatgg gactatgcta 14220
gtaaatccaa actacaaggc tattcaacac ctgtcaagag cgttcccctc ggtcacaggg 14280
tttatcaccc aagtaacttc gtctttttca tctgagctct acctccgatt ctccaaacga 14340
gggaagtttt tcagagatgc tgagtacttg acctcttcca cccttcgaga aatgagcctt 14400
gtgttattca attgtagcag ccccaagagt gagatgcaga gagctcgttc cttgaactat 14460
caggatcttg tgagaggatt tcctgaagaa atcatatcaa atccttacaa tgagatgatc 14520
ataactctga ttgacagtga tgtagaatct tttctagtcc acaagatggt tgatgatctt 14580
gagttacaga ggggaactct gtctaaagtg gctatcatta tagccatcat gatagttttc 14640
tccaacagag tcttcaacgt ttccaaaccc ctaactgacc cctcgttcta tccaccgtct 14700
gatcccaaaa tcctgaggca cttcaacata tgttgcagta ctatgatgta tctatctact 14760
gctttaggtg acgtccctag cttcgcaaga cttcacgacc tgtataacag acctataact 14820
tattacttca gaaagcaagt cattcgaggg aacgtttatc tatcttggag ttggtccaac 14880
gacacctcag tgttcaaaag ggtagcctgt aattctagcc tgagtctgtc atctcactgg 14940
atcaggttga tttacaagat agtgaagact accagactcg ttggcagcat caaggatcta 15000
tccagagaag tggaaagaca ccttcatagg tacaacaggt ggatcaccct agaggatatc 15060
agatctagat catccctact agactacagt tgcctgtgaa ccggatactc ctggaagcct 15120
gcccatgcta agactcttgt gtgatgtatc ttgaaaaaaa caagatccta aatctgaacc 15180
tttggttgtt tgattgtttt tctcattttt gttgtttatt tgttaagcgt gggtcggcat 15240
ggcatctcca cctcctcgcg gtccgacctg ggcatccgaa ggaggacgca cgtccactcg 15300
gatggctaag ggagagccag aaggatccgg ctgctaacaa agcccgaaag gaagctgagt 15360
tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct 15420
tgaggggttt tttgctgaaa gtcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg 15480
tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 15540
gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 15600
ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 15660
ggcggtttgc gtattgggcg ctcttacgct tcctcgctca ctgactcgct gcgctcggtc 15720
gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 15780
tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 15840
aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 15900
aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 15960
ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 16020
tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 16080
agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 16140
gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 16200
tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 16260
acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 16320
tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 16380
caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 16440
aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 16500
aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 16560
ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 16620
agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 16680
atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 16740
cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 16800
aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 16860
cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 16920
aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 16980
ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 17040
gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 17100
ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 17160
tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 17220
tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 17280
ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 17340
tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 17400
agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 17460
acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 17520
ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 17580
gttccgcgca catttccccg aaaagtgcca c 17611
<210> 3
<211> 22946
<212> DNA
<213> 人工序列
<220>
<223> MV-coWuhan-S 第2位
<400> 3
ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 60
cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 120
cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 180
tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 240
gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 300
tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 360
ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 420
taacgcgaat tttaacaaaa tattaacgtt tacaatttcg cgccattcgc cattcaggct 480
gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 540
agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 600
ttgtaaaacg acggccagtg cgcgccgtta atacgactca ctatagggag acccaagctg 660
gctagctttg tttggtctga tgagtcccgt gaggacgaaa cccggcgtac cgggtcacca 720
aacaaagttg ggtaaggata gttcaatcaa tgatcatctt ctagtgcact taggattcaa 780
gatcctatta tcagggacaa gagcaggatt agggatatcc gagatggcca cacttttaag 840
gagcttagca ttgttcaaaa gaaacaagga caaaccaccc attacatcag gatccggtgg 900
agccatcaga ggaatcaaac acattattat agtaccaatc cctggagatt cctcaattac 960
cactcgatcc agacttctgg accggttggt gaggttaatt ggaaacccgg atgtgagcgg 1020
gcccaaacta acaggggcac taataggtat attatcctta tttgtggagt ctccaggtca 1080
attgattcag aggatcaccg atgaccctga cgttagcata aggctgttag aggttgtcca 1140
gagtgaccag tcacaatctg gccttacctt cgcatcaaga ggtaccaaca tggaggatga 1200
ggcggaccaa tacttttcac atgatgatcc aattagtagt gatcaatcca ggttcggatg 1260
gttcgggaac aaggaaatct cagatattga agtgcaagac cctgagggat tcaacatgat 1320
tctgggtacc atcctagccc aaatttgggt cttgctcgca aaggcggtta cggccccaga 1380
cacggcagct gattcggagc taagaaggtg gataaagtac acccaacaaa gaagggtagt 1440
tggtgaattt agattggaga gaaaatggtt ggatgtggtg aggaacagga ttgccgagga 1500
cctctcctta cgccgattca tggtcgctct aatcctggat atcaagagaa cacccggaaa 1560
caaacccagg attgctgaaa tgatatgtga cattgataca tatatcgtag aggcaggatt 1620
agccagtttt atcctgacta ttaagtttgg gatagaaact atgtatcctg ctcttggact 1680
gcatgaattt gctggtgagt tatccacact tgagtccttg atgaaccttt accagcaaat 1740
gggggaaact gcaccctaca tggtaatcct ggagaactca attcagaaca agttcagtgc 1800
aggatcatac cctctgctct ggagctatgc catgggagta ggagtggaac ttgaaaactc 1860
catgggaggt ttgaactttg gccgatctta ctttgatcca gcatatttta gattagggca 1920
agagatggta aggaggtcag ctggaaaggt cagttccaca ttggcatctg aactcggtat 1980
cactgccgag gatgcaaggc ttgtttcaga gattgcaatg catactactg aggacaagat 2040
cagtagagcg gttggaccca gacaagccca agtatcattt ctacacggtg atcaaagtga 2100
gaatgagcta ccgagattgg ggggcaagga agataggagg gtcaaacaga gtcgaggaga 2160
agccagggag agctacagag aaaccgggcc cagcagagca agtgatgcga gagctgccca 2220
tcttccaacc ggcacacccc tagacattga cactgcaacg gagtccagcc aagatccgca 2280
ggacagtcga aggtcagctg acgccctgct taggctgcaa gccatggcag gaatctcgga 2340
agaacaaggc tcagacacgg acacccctat agtgtacaat gacagaaatc ttctagacta 2400
ggtgcgagag gccgagggcc agaacaacat ccgcctacca tccatcattg ttataaaaaa 2460
cttaggaacc aggtccacac agagtgatac gcgtacgcca ccatgttcgt gtttctggtg 2520
ctgctgcctc tggtgagctc ccagtgcgtg aacctgacca caaggaccca gctgccccct 2580
gcctatacca attccttcac acggggcgtg tactatcccg acaaggtgtt ccggagcagc 2640
gtgctgcact ccacacagga tctgtttctg cctttctttt ctaacgtgac ctggttccac 2700
gccatccacg tgagcggcac caatggcaca aagcggttcg acaatccagt gctgcccttt 2760
aacgatggcg tgtacttcgc ctccaccgag aagtctaaca tcatcagagg ctggatcttt 2820
ggcaccacac tggacagcaa gacacagtcc ctgctgatcg tgaacaatgc caccaacgtg 2880
gtcatcaagg tgtgcgagtt ccagttttgt aatgatccat tcctgggcgt gtactatcac 2940
aagaacaata agtcttggat ggagagcgag tttcgcgtgt attcctctgc caacaattgc 3000
acatttgagt acgtgtccca gcccttcctg atggacctgg agggcaagca gggcaatttc 3060
aagaacctga gggagttcgt gtttaagaat atcgatggct acttcaaaat ctactccaag 3120
cacaccccaa tcaacctggt gcgcgacctg ccacagggct tctctgccct ggagccactg 3180
gtggatctgc ccatcggcat caacatcacc cggtttcaga cactgctggc cctgcacaga 3240
agctacctga caccaggcga cagctcctct ggatggaccg caggagcagc agcctactat 3300
gtgggctatc tgcagcccag gaccttcctg ctgaagtaca acgagaatgg caccatcaca 3360
gacgccgtgg attgcgccct ggatcccctg tctgagacca agtgtacact gaagagcttt 3420
accgtggaga agggcatcta tcagacaagc aatttcaggg tgcagcctac cgagtccatc 3480
gtgcgctttc ccaatatcac aaacctgtgc ccttttggcg aggtgttcaa cgcaacccgc 3540
ttcgccagcg tgtacgcctg gaataggaag cgcatctcca actgcgtggc cgactattct 3600
gtgctgtaca acagcgcctc cttctctacc tttaagtgct atggcgtgag ccccacaaag 3660
ctgaatgacc tgtgctttac caacgtgtac gccgattcct tcgtgatcag gggcgacgag 3720
gtgcgccaga tcgcaccagg acagacaggc aagatcgcag actacaatta taagctgcct 3780
gacgatttca ccggctgcgt gatcgcctgg aactctaaca atctggatag caaagtgggc 3840
ggcaactaca attatctgta ccggctgttt agaaagtcta atctgaagcc attcgagagg 3900
gacatctcca cagaaatcta ccaggccggc tctaccccct gcaatggcgt ggagggcttt 3960
aactgttatt tccctctgca gagctacggc ttccagccaa caaacggcgt gggctatcag 4020
ccctaccgcg tggtggtgct gtcttttgag ctgctgcacg cacctgcaac agtgtgcgga 4080
ccaaagaaga gcaccaatct ggtgaagaac aagtgcgtga acttcaactt caacggactg 4140
accggaacag gcgtgctgac cgagtccaac aagaagttcc tgccttttca gcagttcggc 4200
agggacatcg cagataccac agacgccgtg cgcgaccctc agaccctgga gatcctggac 4260
atcacaccat gctccttcgg cggcgtgtct gtgatcacac caggcaccaa tacaagcaac 4320
caggtggccg tgctgtatca ggacgtgaat tgtaccgagg tgccagtggc aatccacgca 4380
gatcagctga cccctacatg gcgggtgtac tctaccggca gcaacgtgtt ccagacaaga 4440
gccggatgcc tgatcggagc agagcacgtg aacaatagct atgagtgcga catccctatc 4500
ggcgccggca tctgtgcctc ctaccagacc cagacaaact ccccaaggag agcacggtct 4560
gtggccagcc agtccatcat cgcctatacc atgagcctgg gcgccgagaa ttccgtggcc 4620
tactccaaca attctatcgc catccctacc aacttcacaa tctccgtgac cacagagatc 4680
ctgccagtga gcatgaccaa gacatccgtg gactgcacaa tgtatatctg tggcgattcc 4740
accgagtgct ctaacctgct gctgcagtac ggctcttttt gtacccagct gaatagagcc 4800
ctgacaggca tcgccgtgga gcaggacaag aacacacagg aggtgttcgc ccaggtgaag 4860
caaatctaca agaccccacc catcaaggac tttggcggct tcaacttcag ccagatcctg 4920
cccgatccta gcaagccatc caagcggtct tttatcgagg acctgctgtt caacaaggtg 4980
accctggccg atgccggctt catcaagcag tatggcgatt gcctgggcga catcgccgcc 5040
agagacctga tctgtgccca gaagtttaat ggcctgaccg tgctgcctcc actgctgaca 5100
gatgagatga tcgcccagta cacatctgcc ctgctggccg gaaccatcac aagcggatgg 5160
accttcggcg caggagccgc cctgcagatc ccctttgcca tgcagatggc ctatcggttc 5220
aacggcatcg gcgtgaccca gaatgtgctg tacgagaacc agaagctgat cgccaatcag 5280
tttaactccg ccatcggcaa gatccaggac tctctgagct ccacagccag cgccctgggc 5340
aagctgcagg atgtggtgaa tcagaacgcc caggccctga ataccctggt gaagcagctg 5400
tctagcaact tcggcgccat ctcctctgtg ctgaatgaca tcctgagccg gctggacaag 5460
gtggaggcag aggtgcagat cgaccggctg atcacaggca gactgcagtc cctgcagacc 5520
tacgtgacac agcagctgat cagggcagca gagatcaggg cctctgccaa tctggccgcc 5580
accaagatga gcgagtgcgt gctgggccag tccaagagag tggacttttg tggcaagggc 5640
tatcacctga tgagcttccc acagtccgcc cctcacggag tggtgtttct gcacgtgacc 5700
tacgtgccag cccaggagaa gaacttcacc acagcaccag caatctgcca cgatggcaag 5760
gcacactttc ctagggaggg cgtgttcgtg agcaacggca cccactggtt tgtgacacag 5820
cgcaatttct acgagccaca gatcatcacc acagacaata cattcgtgtc cggcaactgt 5880
gacgtggtca tcggcatcgt gaacaatacc gtgtatgatc ctctgcagcc agagctggac 5940
tcttttaagg aggagctgga taagtacttc aagaatcaca ccagccccga cgtggatctg 6000
ggcgacatct ctggcatcaa tgccagcgtg gtgaacatcc agaaggagat cgacaggctg 6060
aacgaggtgg ccaagaatct gaacgagtcc ctgatcgatc tgcaggagct gggcaagtat 6120
gagcagtaca tcaagtggcc ctggtatatc tggctgggct tcatcgccgg cctgatcgcc 6180
atcgtgatgg tgaccatcat gctgtgctgt atgacaagct gctgttcctg cctgaagggc 6240
tgctgttctt gtggcagctg ctgtaagttt gatgaggacg atagcgagcc tgtgctgaag 6300
ggcgtgaagc tgcactacac ctgatagcta gcgatcgcgt gcgagaggcc agaacaacat 6360
ccgcctacca tccatcattg ttataaaaaa cttaggaacc aggtccacac agccgccagc 6420
ccatcaacca tccactccca cgattggagc caatggcaga agagcaggca cgccatgtca 6480
aaaacggact ggaatgcatc cgggctctca aggccgagcc catcggctca ctggccatcg 6540
aggaagctat ggcagcatgg tcagaaatat cagacaaccc aggacaggag cgagccacct 6600
gcagggaaga gaaggcaggc agttcgggtc tcagcaaacc atgcctctca gcaattggat 6660
caactgaagg cggtgcacct cgcatccgcg gtcagggacc tggagagagc gatgacgacg 6720
ctgaaacttt gggaatcccc ccaagaaatc tccaggcatc aagcactggg ttacagtgtt 6780
attacgttta tgatcacagc ggtgaagcgg ttaagggaat ccaagatgct gactctatca 6840
tggttcaatc aggccttgat ggtgatagca ccctctcagg aggagacaat gaatctgaaa 6900
acagcgatgt ggatattggc gaacctgata ccgagggata tgctatcact gaccggggat 6960
ctgctcccat ctctatgggg ttcagggctt ctgatgttga aactgcagaa ggaggggaga 7020
tccacgagct cctgagactc caatccagag gcaacaactt tccgaagctt gggaaaactc 7080
tcaatgttcc tccgcccccg gaccccggta gggccagcac ttccgggaca cccattaaaa 7140
agggcacaga cgcgagatta gcctcatttg gaacggagat cgcgtcttta ttgacaggtg 7200
gtgcaaccca atgtgctcga aagtcaccct cggaaccatc agggccaggt gcacctgcgg 7260
ggaatgtccc cgagtgtgtg agcaatgccg cactgataca ggagtggaca cccgaatctg 7320
gtaccacaat ctccccgaga tcccagaata atgaagaagg gggagactat tatgatgatg 7380
agctgttctc tgatgtccaa gatattaaaa cagccttggc caaaatacac gaggataatc 7440
agaagataat ctccaagcta gaatcactgc tgttattgaa gggagaagtt gagtcaatta 7500
agaagcagat caacaggcaa aatatcagca tatccaccct ggaaggacac ctctcaagca 7560
tcatgatcgc cattcctgga cttgggaagg atcccaacga ccccactgca gatgtcgaaa 7620
tcaatcccga cttgaaaccc atcataggca gagattcagg ccgagcactg gccgaagttc 7680
tcaagaaacc cgttgccagc cgacaactcc aaggaatgac aaatggacgg accagttcca 7740
gaggacagct gctgaaggaa tttcagctaa agccgatcgg gaaaaagatg agctcagccg 7800
tcgggtttgt tcctgacacc ggccctgcat cacgcagtgt aatccgctcc attataaaat 7860
ccagccggct agaggaggat cggaagcgtt acctgatgac tctccttgat gatatcaaag 7920
gagccaatga tcttgccaag ttccaccaga tgctgatgaa gataataatg aagtagctac 7980
agctcaactt acctgccaac cccatgccag tcgacccacc tagtacaacc taaatccatt 8040
ataaaaaact taggagcaaa gtgattgcct cccaaggtcc acaatgacag agacctacga 8100
cttcgacaag tcggcatggg acatcaaagg gtcgatcgct ccgatacaac ccaccaccta 8160
cagtgatggc aggctggtgc cccaggtcag agtcatagat cctggtctag gcgacaggaa 8220
ggatgaatgc tttatgtaca tgtttctgct gggggttgtt gaggacagcg attccctagg 8280
gcctccaatc gggcgagcat ttgggttcct gcccttaggt gttggcagat ccacagcaaa 8340
gcccgaaaaa ctcctcaaag aggccactga gcttgacata gttgttagac gtacagcagg 8400
gctcaatgaa aaactggtgt tctacaacaa caccccacta actctcctca caccttggag 8460
aaaggtccta acaacaggga gtgtcttcaa cgcaaaccaa gtgtgcaatg cggttaatct 8520
gataccgctc gataccccgc agaggttccg tgttgtttat atgagcatca cccgtctttc 8580
ggataacggg tattacaccg ttcctagaag aatgctggaa ttcagatcgg tcaatgcagt 8640
ggccttcaac ctgctggtga cccttaggat tgacaaggcg ataggccctg ggaagatcat 8700
cgacaataca gagcaacttc ctgaggcaac atttatggtc cacatcggga acttcaggag 8760
aaagaagagt gaagtctact ctgccgatta ttgcaaaatg aaaatcgaaa agatgggcct 8820
ggtttttgca cttggtggga tagggggcac cagtcttcac attagaagca caggcaaaat 8880
gagcaagact ctccatgcac aactcgggtt caagaagacc ttatgttacc cgctgatgga 8940
tatcaatgaa gaccttaatc gattactctg gaggagcaga tgcaagatag taagaatcca 9000
ggcagttttg cagccatcag ttcctcaaga attccgcatt tacgacgacg tgatcataaa 9060
tgatgaccaa ggactattca aagttctgta gaccgtagtg cccagcaatg cccgaaaacg 9120
acccccctca caatgacagc cagaaggccc ggacaaaaaa gccccctccg aaagactcca 9180
cggaccaagc gagaggccag ccagcagccg acggcaagcg cgaacaccag gcggccccag 9240
cacagaacag ccctgacaca aggccaccac cagccacccc aatctgcatc ctcctcgtgg 9300
gacccccgag gaccaacccc caaggctgcc cccgatccaa accaccaacc gcatccccac 9360
cacccccggg aaagaaaccc ccagcaattg gaaggcccct ccccctcttc ctcaacacaa 9420
gaactccaca accgaaccgc acaagcgacc gaggtgaccc aaccgcaggc atccgactcc 9480
ctagacagat cctctctccc cggcaaacta aacaaaactt agggccaagg aacatacaca 9540
cccaacagaa cccagacccc ggcccacggc gccgcgcccc caacccccga caaccagagg 9600
gagcccccaa ccaatcccgc cggctccccc ggtgcccaca ggcagggaca ccaacccccg 9660
aacagaccca gcacccaacc atcgacaatc caagacgggg gggccccccc aaaaaaaggc 9720
ccccaggggc cgacagccag caccgcgagg aagcccaccc accccacaca cgaccacggc 9780
aaccaaacca gaacccagac caccctgggc caccagctcc cagactcggc catcaccccg 9840
cagaaaggaa aggccacaac ccgcgcaccc cagccccgat ccggcgggga gccacccaac 9900
ccgaaccagc acccaagagc gatccccgaa ggacccccga accgcaaagg acatcagtat 9960
cccacagcct ctccaagtcc cccggtctcc tcctcttctc gaagggacca aaagatcaat 10020
ccaccacacc cgacgacact caactcccca cccctaaagg agacaccggg aatcccagaa 10080
tcaagactca tccaatgtcc atcatgggtc tcaaggtgaa cgtctctgcc atattcatgg 10140
cagtactgtt aactctccaa acacccaccg gtcaaatcca ttggggcaat ctctctaaga 10200
taggggtggt aggaatagga agtgcaagct acaaagttat gactcgttcc agccatcaat 10260
cattagtcat aaaattaatg cccaatataa ctctcctcaa taactgcacg agggtagaga 10320
ttgcagaata caggagacta ctgagaacag ttttggaacc aattagagat gcacttaatg 10380
caatgaccca gaatataaga ccggttcaga gtgtagcttc aagtaggaga cacaagagat 10440
ttgcgggagt agtcctggca ggtgcggccc taggcgttgc cacagctgct cagataacag 10500
ccggcattgc acttcaccag tccatgctga actctcaagc catcgacaat ctgagagcga 10560
gcctggaaac tactaatcag gcaattgaga caatcagaca agcagggcag gagatgatat 10620
tggctgttca gggtgtccaa gactacatca ataatgagct gataccgtct atgaaccaac 10680
tatcttgtga tttaatcggc cagaagctcg ggctcaaatt gctcagatac tatacagaaa 10740
tcctgtcatt atttggcccc agtttacggg accccatatc tgcggagata tctatccagg 10800
ctttgagcta tgcgcttgga ggagacatca ataaggtgtt agaaaagctc ggatacagtg 10860
gaggtgattt actgggcatc ttagagagcg gaggaataaa ggcccggata actcacgtcg 10920
acacagagtc ctacttcatt gtcctcagta tagcctatcc gacgctgtcc gagattaagg 10980
gggtgattgt ccaccggcta gagggggtct cgtacaacat aggctctcaa gagtggtata 11040
ccactgtgcc caagtatgtt gcaacccaag ggtaccttat ctcgaatttt gatgagtcat 11100
cgtgtacttt catgccagag gggactgtgt gcagccaaaa tgccttgtac ccgatgagtc 11160
ctctgctcca agaatgcctc cgggggtaca ccaagtcctg tgctcgtaca ctcgtatccg 11220
ggtcttttgg gaaccggttc attttatcac aagggaacct aatagccaat tgtgcatcaa 11280
tcctttgcaa gtgttacaca acaggaacga tcattaatca agaccctgac aagatcctaa 11340
catacattgc tgccgatcac tgcccggtag tcgaggtgaa cggcgtgacc atccaagtcg 11400
ggagcaggag gtatccagac gctgtgtact tgcacagaat tgacctcggt cctcccatat 11460
cattggagag gttggacgta gggacaaatc tggggaatgc aattgctaag ttggaggatg 11520
ccaaggaatt gttggagtca tcggaccaga tattgaggag tatgaaaggt ttatcgagca 11580
ctagcatagt ctacatcctg attgcagtgt gtcttggagg gttgataggg atccccgctt 11640
taatatgttg ctgcaggggg cgttgtaaca aaaagggaga acaagttggt atgtcaagac 11700
caggcctaaa gcctgatctt acgggaacat caaaatccta tgtaaggtcg ctctgatcct 11760
ctacaactct tgaaacacaa atgtcccaca agtctcctct tcgtcatcaa gcaaccaccg 11820
cacccagcat caagcccacc tgaaattatc tccggcttcc ctctggccga acaatatcgg 11880
tagttaatta aaacttaggg tgcaagatca tcgataatgt caccacaacg agaccggata 11940
aatgccttct acaaagataa cccccatccc aagggaagta ggatagtcat taacagagaa 12000
catcttatga ttgatagacc ttatgttttg ctggctgttc tgtttgtcat gtttctgagc 12060
ttgatcgggt tgctagccat tgcaggaatt cgacttcatc gggcagccat ctacaccgca 12120
gagatccata aaagcctcag caccaatcta gatgtaacta actcaatcga gcatcaggtc 12180
aaggacgtgc tgacaccact cttcaaaatc atcggtgatg aagtgggcct gaggacacct 12240
cagagattca ctgacctagt gaaattaatc tctgacaaga ttaaattcct taatccggat 12300
agggagtacg acttcagaga tctcacttgg tgtatcaacc cgccagagag aatcaaattg 12360
gattatgatc aatactgtgc agatgtggct gctgaagagc tcatgaatgc attggtgaac 12420
tcaactctac tggagaccag aacaaccaat cagttcctag ctgtctcaaa gggaaactgc 12480
tcagggccca ctacaatcag aggtcaattc tcaaacatgt cgctgtccct gttagacttg 12540
tatttaggtc gaggttacaa tgtgtcatct atagtcacta tgacatccca gggaatgtat 12600
gggggaactt acctagtgga aaagcctaat ctgagcagca aaaggtcaga gttgtcacaa 12660
ctgagcatgt accgagtgtt tgaagtaggt gttatcagaa atccgggttt gggggctccg 12720
gtgttccata tgacaaacta tcttgagcaa ccagtcagta atgatctcag caactgtatg 12780
gtggctttgg gggagctcaa actcgcagcc ctttgtcacg gggaagattc tatcacaatt 12840
ccctatcagg gatcagggaa aggtgtcagc ttccagctcg tcaagctagg tgtctggaaa 12900
tccccaaccg acatgcaatc ctgggtcccc ttatcaacgg atgatccagt gatagacagg 12960
ctttacctct catctcacag aggtgttatc gctgacaacc aagcaaaatg ggctgtcccg 13020
acaacacgaa cagatgacaa gttgcgaatg gagacatgct tccaacaggc gtgtaagggt 13080
aaaatccaag cactctgcga gaatcccgag tgggcaccat tgaaggataa caggattcct 13140
tcatacgggg tcttgtctgt tgatctgagt ctgacagttg agcttaaaat caaaattgct 13200
tcgggattcg ggccattgat cacacacggt tcagggatgg acctatacaa atccaaccac 13260
aacaatgtgt attggctgac tatcccgcca atgaagaacc tagccttagg tgtaatcaac 13320
acattggagt ggataccgag attcaaggtt agtccctacc tcttcactgt cccaattaag 13380
gaagcaggcg aagactgcca tgccccaaca tacctacctg cggaggtgga tggtgatgtc 13440
aaactcagtt ccaatctggt gattctacct ggtcaagatc tccaatatgt tttggcaacc 13500
tacgatactt ccagggttga acatgctgtg gtttattacg tttacagccc aagccgctca 13560
ttttcttact tttatccttt taggttgcct ataaaggggg tccccatcga attacaagtg 13620
gaatgcttca catgggacca aaaactctgg tgccgtcact tctgtgtgct tgcggactca 13680
gaatctggtg gacatatcac tcactctggg atggtgggca tgggagtcag ctgcacagtc 13740
acccgggaag atggaaccaa tcgcagatag ggctgctagt gaaccaatca catgatgtca 13800
cccagacatc aggcataccc actagtgtga aatagacatc agaattaaga aaaacgtagg 13860
gtccaagtgg ttccccgtta tggactcgct atctgtcaac cagatcttat accctgaagt 13920
tcacctagat agcccgatag ttaccaataa gatagtagcc atcctggagt atgctcgagt 13980
ccctcacgct tacagcctgg aggaccctac actgtgtcag aacatcaagc accgcctaaa 14040
aaacggattt tccaaccaaa tgattataaa caatgtggaa gttgggaatg tcatcaagtc 14100
caagcttagg agttatccgg cccactctca tattccatat ccaaattgta atcaggattt 14160
atttaacata gaagacaaag agtcaacgag gaagatccgt gaactcctca aaaaggggaa 14220
ttcgctgtac tccaaagtca gtgataaggt tttccaatgc ttaagggaca ctaactcacg 14280
gcttggccta ggctccgaat tgagggagga catcaaggag aaagttatta acttgggagt 14340
ttacatgcac agctcccagt ggtttgagcc ctttctgttt tggtttacag tcaagactga 14400
gatgaggtca gtgattaaat cacaaaccca tacttgccat aggaggagac acacacctgt 14460
attcttcact ggtagttcag ttgagttgct aatctctcgt gaccttgttg ctataatcag 14520
taaagagtct caacatgtat attacctgac atttgaactg gttttgatgt attgtgatgt 14580
catagagggg aggttaatga cagagaccgc tatgactatt gatgctaggt atacagagct 14640
tctaggaaga gtcagataca tgtggaaact gatagatggt ttcttccctg cactcgggaa 14700
tccaacttat caaattgtag ccatgctgga gcctctttca cttgcttacc tgcagctgag 14760
ggatataaca gtagaactca gaggtgcttt ccttaaccac tgctttactg aaatacatga 14820
tgttcttgac caaaacgggt tttctgatga aggtacttat catgagttaa ctgaagctct 14880
agattacatt ttcataactg atgacataca tctgacaggg gagattttct catttttcag 14940
aagtttcggc caccccagac ttgaagcagt aacggctgct gaaaatgtta ggaaatacat 15000
gaatcagcct aaagtcattg tgtatgagac tctgatgaaa ggtcatgcca tattttgtgg 15060
aatcataatc aacggctatc gtgacaggca cggaggcagt tggccaccgc tgaccctccc 15120
cctgcatgct gcagacacaa tccggaatgc tcaagcttca ggtgaagggt taacacatga 15180
gcagtgcgtt gataactgga aatcttttgc tggagtgaaa tttggctgct ttatgcctct 15240
tagcctggat agtgatctga caatgtacct aaaggacaag gcacttgctg ctctccaaag 15300
ggaatgggat tcagtttacc cgaaagagtt cctgcgttac gaccctccca agggaaccgg 15360
gtcacggagg cttgtagatg ttttccttaa tgattcgagc tttgacccat atgatgtgat 15420
aatgtatgtt gtaagtggag cttacctcca tgaccctgag ttcaacctgt cttacagcct 15480
gaaagaaaag gagatcaagg aaacaggtag actttttgct aaaatgactt acaaaatgag 15540
ggcatgccaa gtgattgctg aaaatctaat ctcaaacggg attggcaaat attttaagga 15600
caatgggatg gccaaggatg agcacgattt gactaaggca ctccacactc tagctgtctc 15660
aggagtcccc aaagatctca aagaaagtca cagggggggg ccagtcttaa aaacctactc 15720
ccgaagccca gtccacacaa gtaccaggaa cgtgagagca gcaaaagggt ttatagggtt 15780
ccctcaagta attcggcagg accaagacac tgatcatccg gagaatatgg aagcttacga 15840
gacagtcagt gcatttatca cgactgatct caagaagtac tgccttaatt ggagatatga 15900
gaccatcagc ttgtttgcac agaggctaaa tgagatttac ggattgccct catttttcca 15960
gtggctgcat aagaggcttg agacctctgt cctgtatgta agtgaccctc attgcccccc 16020
cgaccttgac gcccatatcc cgttatataa agtccccaat gatcaaatct tcattaagta 16080
ccctatggga ggtatagaag ggtattgtca gaagctgtgg accatcagca ccattcccta 16140
tctatacctg gctgcttatg agagcggagt aaggattgct tcgttagtgc aaggggacaa 16200
tcagaccata gccgtaacaa aaagggtacc cagcacatgg ccctacaacc ttaagaaacg 16260
ggaagctgct agagtaacta gagattactt tgtaattctt aggcaaaggc tacatgatat 16320
tggccatcac ctcaaggcaa atgagacaat tgtttcatca catttttttg tctattcaaa 16380
aggaatatat tatgatgggc tacttgtgtc ccaatcactc aagagcatcg caagatgtgt 16440
attctggtca gagactatag ttgatgaaac aagggcagca tgcagtaata ttgctacaac 16500
aatggctaaa agcatcgaga gaggttatga ccgttacctt gcatattccc tgaacgtcct 16560
aaaagtgata cagcaaattc tgatctctct tggcttcaca atcaattcaa ccatgacccg 16620
ggatgtagtc atacccctcc tcacaaacaa cgacctctta ataaggatgg cactgttgcc 16680
cgctcctatt ggggggatga attatctgaa tatgagcagg ctgtttgtca gaaacatcgg 16740
tgatccagta acatcatcaa ttgctgatct caagagaatg attctcgcct cactaatgcc 16800
tgaagagacc ctccatcagg taatgacaca acaaccgggg gactcttcat tcctagactg 16860
ggctagcgac ccttactcag caaatcttgt atgtgtccag agcatcacta gactcctcaa 16920
gaacataact gcaaggtttg tcctgatcca tagtccaaac ccaatgttaa aaggattatt 16980
ccatgatgac agtaaagaag aggacgaggg actggcggca ttcctcatgg acaggcatat 17040
tatagtacct agggcagctc atgaaatcct ggatcatagt gtcacagggg caagagagtc 17100
tattgcaggc atgctggata ccacaaaagg cttgattcga gccagcatga ggaagggggg 17160
tttaacctct cgagtgataa ccagattgtc caattatgac tatgaacaat tcagagcagg 17220
gatggtgcta ttgacaggaa gaaagagaaa tgtcctcatt gacaaagagt catgttcagt 17280
gcagctggcg agagctctaa gaagccatat gtgggcgagg ctagctcgag gacggcctat 17340
ttacggcctt gaggtccctg atgtactaga atctatgcga ggccacctta ttcggcgtca 17400
tgagacatgt gtcatctgcg agtgtggatc agtcaactac ggatggtttt ttgtcccctc 17460
gggttgccaa ctggatgata ttgacaagga aacatcatcc ttgagagtcc catatattgg 17520
ttctaccact gatgagagaa cagacatgaa gcttgccttc gtaagagccc caagtcgatc 17580
cttgcgatct gctgttagaa tagcaacagt gtactcatgg gcttacggtg atgatgatag 17640
ctcttggaac gaagcctggt tgttggctag gcaaagggcc aatgtgagcc tggaggagct 17700
aagggtgatc actcccatct caacttcgac taatttagcg cataggttga gggatcgtag 17760
cactcaagtg aaatactcag gtacatccct tgtccgagtg gcgaggtata ccacaatctc 17820
caacgacaat ctctcatttg tcatatcaga taagaaggtt gatactaact ttatatacca 17880
acaaggaatg cttctagggt tgggtgtttt agaaacattg tttcgactcg agaaagatac 17940
cggatcatct aacacggtat tacatcttca cgtcgaaaca gattgttgcg tgatcccgat 18000
gatagatcat cccaggatac ccagctcccg caagctagag ctgagggcag agctatgtac 18060
caacccattg atatatgata atgcaccttt aattgacaga gatgcaacaa ggctatacac 18120
ccagagccat aggaggcacc ttgtggaatt tgttacatgg tccacacccc aactatatca 18180
cattttagct aagtccacag cactatctat gattgacctg gtaacaaaat ttgagaagga 18240
ccatatgaat gaaatttcag ctctcatagg ggatgacgat atcaatagtt tcataactga 18300
gtttctgctc atagagccaa gattattcac tatctacttg ggccagtgtg cggccatcaa 18360
ttgggcattt gatgtacatt atcatagacc atcagggaaa tatcagatgg gtgagctgtt 18420
gtcatcgttc ctttctagaa tgagcaaagg agtgtttaag gtgcttgtca atgctctaag 18480
ccacccaaag atctacaaga aattctggca ttgtggtatt atagagccta tccatggtcc 18540
ttcacttgat gctcaaaact tgcacacaac tgtgtgcaac atggtttaca catgctatat 18600
gacctacctc gacctgttgt tgaatgaaga gttagaagag ttcacatttc tcttgtgtga 18660
aagcgacgag gatgtagtac cggacagatt cgacaacatc caggcaaaac acttatgtgt 18720
tctggcagat ttgtactgtc aaccagggac ctgcccacca attcgaggtc taagaccggt 18780
agagaaatgt gcagttctaa ccgaccatat caaggcagag gctatgttat ctccagcagg 18840
atcttcgtgg aacataaatc caattattgt agaccattac tcatgctccc tgacttatct 18900
ccggcgagga tcgatcaaac agataagatt gagagttgat ccaggattca ttttcgacgc 18960
cctcgctgag gtaaatgtca gtcagccaaa gatcggcagc aacaacatct caaatatgag 19020
catcaaggct ttcagacccc cacacgatga tgttgcaaaa ttgctcaaag atatcaacac 19080
aagcaagcac aatcttccca tttcaggggg caatctcgcc aattatgaaa tccatgcttt 19140
ccgcagaatc gggttgaact catctgcttg ctacaaagct gttgagatat caacattaat 19200
taggagatgc cttgagccag gggaggacgg cttgttcttg ggtgagggat cgggttctat 19260
gttgatcact tataaggaga tacttaaact aaacaagtgc ttctataata gtggggtttc 19320
cgccaattct agatctggtc aaagggaatt agcaccctat ccctccgaag ttggccttgt 19380
cgaacacaga atgggagtag gtaatattgt caaagtgctc tttaacggga ggcccgaagt 19440
cacgtgggta ggcagtgtag attgcttcaa tttcatagtt agtaatatcc ctacctctag 19500
tgtggggttt atccattcag atatagagac cttgcctgac aaagatacta tagagaagct 19560
agaggaattg gcagccatct tatcgatggc tctgctcctg ggcaaaatag gatcaatact 19620
ggtgattaag cttatgcctt tcagcgggga ttttgttcag ggatttataa gttatgtagg 19680
gtctcattat agagaagtga accttgtata ccctagatac agcaacttca tatctactga 19740
atcttatttg gttatgacag atctcaaggc taaccggcta atgaatcctg aaaagattaa 19800
gcagcagata attgaatcat ctgtgaggac ttcacctgga cttataggtc acatcctatc 19860
cattaagcaa ctaagctgca tacaagcaat tgtgggagac gcagttagta gaggtgatat 19920
caatcctact ctgaaaaaac ttacacctat agagcaggtg ctgatcaatt gcgggttggc 19980
aattaacgga cctaagctgt gcaaagaatt gatccaccat gatgttgcct cagggcaaga 20040
tggattgctt aattctatac tcatcctcta cagggagttg gcaagattca aagacaacca 20100
aagaagtcaa caagggatgt tccacgccta ccccgtattg gtaagtagca ggcaacgaga 20160
acttatatct aggatcaccc gcaaattttg ggggcacatt cttctttact ccgggaacaa 20220
aaagttgata aataagttta tccagaatct caagtccggc tatctgatac tagacttaca 20280
ccagaatatc ttcgttaaga atctatccaa gtcagagaaa cagattatta tgacgggggg 20340
tttgaaacgt gagtgggttt ttaaggtaac agtcaaggag accaaagaat ggtataagtt 20400
agtcggatac agtgccctga ttaaggacta attggttgaa ctccggaacc ctaatcctgc 20460
cctaggtggt taggcattat ttgcaatata ttaaagaaaa ctttgaaaat acgaagtttc 20520
tattcccagc tttgtctggt ggccggcata gtcccagcct cctcgctggc gctggctggg 20580
caacattccg aggggaccgt ccccacggta atggcgaatg ggacgcggcc gatccggctg 20640
ctaacaaagc ccgaaaggaa gctgagttgg ctgctggcgc tggctgggca ataactagca 20700
taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata 20760
tccggatgcg gccgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 20820
ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 20880
tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 20940
gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 21000
gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 21060
gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 21120
taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 21180
cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 21240
ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 21300
aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 21360
tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 21420
gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 21480
cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 21540
ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 21600
cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 21660
gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 21720
cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 21780
tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 21840
ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 21900
aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 21960
atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 22020
ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 22080
tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 22140
agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 22200
taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 22260
tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 22320
cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 22380
ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 22440
tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 22500
tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 22560
cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 22620
tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 22680
gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 22740
tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 22800
atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 22860
tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 22920
cacatttccc cgaaaagtgc cacctg 22946
<210> 4
<211> 22916
<212> DNA
<213> 人工序列
<220>
<223> MV Wu S 在第3位
<400> 4
ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 60
cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 120
cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 180
tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 240
gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 300
tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 360
ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 420
taacgcgaat tttaacaaaa tattaacgtt tacaatttcg cgccattcgc cattcaggct 480
gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 540
agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 600
ttgtaaaacg acggccagtg cgcgccgtta atacgactca ctatagggag acccaagctg 660
gctagctttg tttggtctga tgagtcccgt gaggacgaaa cccggcgtac cgggtcacca 720
aacaaagttg ggtaaggata gttcaatcaa tgatcatctt ctagtgcact taggattcaa 780
gatcctatta tcagggacaa gagcaggatt agggatatcc gagatggcca cacttttaag 840
gagcttagca ttgttcaaaa gaaacaagga caaaccaccc attacatcag gatccggtgg 900
agccatcaga ggaatcaaac acattattat agtaccaatc cctggagatt cctcaattac 960
cactcgatcc agacttctgg accggttggt gaggttaatt ggaaacccgg atgtgagcgg 1020
gcccaaacta acaggggcac taataggtat attatcctta tttgtggagt ctccaggtca 1080
attgattcag aggatcaccg atgaccctga cgttagcata aggctgttag aggttgtcca 1140
gagtgaccag tcacaatctg gccttacctt cgcatcaaga ggtaccaaca tggaggatga 1200
ggcggaccaa tacttttcac atgatgatcc aattagtagt gatcaatcca ggttcggatg 1260
gttcgggaac aaggaaatct cagatattga agtgcaagac cctgagggat tcaacatgat 1320
tctgggtacc atcctagccc aaatttgggt cttgctcgca aaggcggtta cggccccaga 1380
cacggcagct gattcggagc taagaaggtg gataaagtac acccaacaaa gaagggtagt 1440
tggtgaattt agattggaga gaaaatggtt ggatgtggtg aggaacagga ttgccgagga 1500
cctctcctta cgccgattca tggtcgctct aatcctggat atcaagagaa cacccggaaa 1560
caaacccagg attgctgaaa tgatatgtga cattgataca tatatcgtag aggcaggatt 1620
agccagtttt atcctgacta ttaagtttgg gatagaaact atgtatcctg ctcttggact 1680
gcatgaattt gctggtgagt tatccacact tgagtccttg atgaaccttt accagcaaat 1740
gggggaaact gcaccctaca tggtaatcct ggagaactca attcagaaca agttcagtgc 1800
aggatcatac cctctgctct ggagctatgc catgggagta ggagtggaac ttgaaaactc 1860
catgggaggt ttgaactttg gccgatctta ctttgatcca gcatatttta gattagggca 1920
agagatggta aggaggtcag ctggaaaggt cagttccaca ttggcatctg aactcggtat 1980
cactgccgag gatgcaaggc ttgtttcaga gattgcaatg catactactg aggacaagat 2040
cagtagagcg gttggaccca gacaagccca agtatcattt ctacacggtg atcaaagtga 2100
gaatgagcta ccgagattgg ggggcaagga agataggagg gtcaaacaga gtcgaggaga 2160
agccagggag agctacagag aaaccgggcc cagcagagca agtgatgcga gagctgccca 2220
tcttccaacc ggcacacccc tagacattga cactgcaacg gagtccagcc aagatccgca 2280
ggacagtcga aggtcagctg acgccctgct taggctgcaa gccatggcag gaatctcgga 2340
agaacaaggc tcagacacgg acacccctat agtgtacaat gacagaaatc ttctagacta 2400
ggtgcgagag gccgagggcc agaacaacat ccgcctacca tccatcattg ttataaaaaa 2460
cttaggaacc aggtccacac agccgccagc ccatcaacca tccactccca cgattggagc 2520
caatggcaga agagcaggca cgccatgtca aaaacggact ggaatgcatc cgggctctca 2580
aggccgagcc catcggctca ctggccatcg aggaagctat ggcagcatgg tcagaaatat 2640
cagacaaccc aggacaggag cgagccacct gcagggaaga gaaggcaggc agttcgggtc 2700
tcagcaaacc atgcctctca gcaattggat caactgaagg cggtgcacct cgcatccgcg 2760
gtcagggacc tggagagagc gatgacgacg ctgaaacttt gggaatcccc ccaagaaatc 2820
tccaggcatc aagcactggg ttacagtgtt attacgttta tgatcacagc ggtgaagcgg 2880
ttaagggaat ccaagatgct gactctatca tggttcaatc aggccttgat ggtgatagca 2940
ccctctcagg aggagacaat gaatctgaaa acagcgatgt ggatattggc gaacctgata 3000
ccgagggata tgctatcact gaccggggat ctgctcccat ctctatgggg ttcagggctt 3060
ctgatgttga aactgcagaa ggaggggaga tccacgagct cctgagactc caatccagag 3120
gcaacaactt tccgaagctt gggaaaactc tcaatgttcc tccgcccccg gaccccggta 3180
gggccagcac ttccgggaca cccattaaaa agggcacaga cgcgagatta gcctcatttg 3240
gaacggagat cgcgtcttta ttgacaggtg gtgcaaccca atgtgctcga aagtcaccct 3300
cggaaccatc agggccaggt gcacctgcgg ggaatgtccc cgagtgtgtg agcaatgccg 3360
cactgataca ggagtggaca cccgaatctg gtaccacaat ctccccgaga tcccagaata 3420
atgaagaagg gggagactat tatgatgatg agctgttctc tgatgtccaa gatattaaaa 3480
cagccttggc caaaatacac gaggataatc agaagataat ctccaagcta gaatcactgc 3540
tgttattgaa gggagaagtt gagtcaatta agaagcagat caacaggcaa aatatcagca 3600
tatccaccct ggaaggacac ctctcaagca tcatgatcgc cattcctgga cttgggaagg 3660
atcccaacga ccccactgca gatgtcgaaa tcaatcccga cttgaaaccc atcataggca 3720
gagattcagg ccgagcactg gccgaagttc tcaagaaacc cgttgccagc cgacaactcc 3780
aaggaatgac aaatggacgg accagttcca gaggacagct gctgaaggaa tttcagctaa 3840
agccgatcgg gaaaaagatg agctcagccg tcgggtttgt tcctgacacc ggccctgcat 3900
cacgcagtgt aatccgctcc attataaaat ccagccggct agaggaggat cggaagcgtt 3960
acctgatgac tctccttgat gatatcaaag gagccaatga tcttgccaag ttccaccaga 4020
tgctgatgaa gataataatg aagtagctac agctcaactt acctgccaac cccatgccag 4080
tcgatcatcc atcattgtta taaaaaactt aggaaccagg tccacacaga gtgatacgcg 4140
tacgccacca tgttcgtgtt tctggtgctg ctgcctctgg tgagctccca gtgcgtgaac 4200
ctgaccacaa ggacccagct gccccctgcc tataccaatt ccttcacacg gggcgtgtac 4260
tatcccgaca aggtgttccg gagcagcgtg ctgcactcca cacaggatct gtttctgcct 4320
ttcttttcta acgtgacctg gttccacgcc atccacgtga gcggcaccaa tggcacaaag 4380
cggttcgaca atccagtgct gccctttaac gatggcgtgt acttcgcctc caccgagaag 4440
tctaacatca tcagaggctg gatctttggc accacactgg acagcaagac acagtccctg 4500
ctgatcgtga acaatgccac caacgtggtc atcaaggtgt gcgagttcca gttttgtaat 4560
gatccattcc tgggcgtgta ctatcacaag aacaataagt cttggatgga gagcgagttt 4620
cgcgtgtatt cctctgccaa caattgcaca tttgagtacg tgtcccagcc cttcctgatg 4680
gacctggagg gcaagcaggg caatttcaag aacctgaggg agttcgtgtt taagaatatc 4740
gatggctact tcaaaatcta ctccaagcac accccaatca acctggtgcg cgacctgcca 4800
cagggcttct ctgccctgga gccactggtg gatctgccca tcggcatcaa catcacccgg 4860
tttcagacac tgctggccct gcacagaagc tacctgacac caggcgacag ctcctctgga 4920
tggaccgcag gagcagcagc ctactatgtg ggctatctgc agcccaggac cttcctgctg 4980
aagtacaacg agaatggcac catcacagac gccgtggatt gcgccctgga tcccctgtct 5040
gagaccaagt gtacactgaa gagctttacc gtggagaagg gcatctatca gacaagcaat 5100
ttcagggtgc agcctaccga gtccatcgtg cgctttccca atatcacaaa cctgtgccct 5160
tttggcgagg tgttcaacgc aacccgcttc gccagcgtgt acgcctggaa taggaagcgc 5220
atctccaact gcgtggccga ctattctgtg ctgtacaaca gcgcctcctt ctctaccttt 5280
aagtgctatg gcgtgagccc cacaaagctg aatgacctgt gctttaccaa cgtgtacgcc 5340
gattccttcg tgatcagggg cgacgaggtg cgccagatcg caccaggaca gacaggcaag 5400
atcgcagact acaattataa gctgcctgac gatttcaccg gctgcgtgat cgcctggaac 5460
tctaacaatc tggatagcaa agtgggcggc aactacaatt atctgtaccg gctgtttaga 5520
aagtctaatc tgaagccatt cgagagggac atctccacag aaatctacca ggccggctct 5580
accccctgca atggcgtgga gggctttaac tgttatttcc ctctgcagag ctacggcttc 5640
cagccaacaa acggcgtggg ctatcagccc taccgcgtgg tggtgctgtc ttttgagctg 5700
ctgcacgcac ctgcaacagt gtgcggacca aagaagagca ccaatctggt gaagaacaag 5760
tgcgtgaact tcaacttcaa cggactgacc ggaacaggcg tgctgaccga gtccaacaag 5820
aagttcctgc cttttcagca gttcggcagg gacatcgcag ataccacaga cgccgtgcgc 5880
gaccctcaga ccctggagat cctggacatc acaccatgct ccttcggcgg cgtgtctgtg 5940
atcacaccag gcaccaatac aagcaaccag gtggccgtgc tgtatcagga cgtgaattgt 6000
accgaggtgc cagtggcaat ccacgcagat cagctgaccc ctacatggcg ggtgtactct 6060
accggcagca acgtgttcca gacaagagcc ggatgcctga tcggagcaga gcacgtgaac 6120
aatagctatg agtgcgacat ccctatcggc gccggcatct gtgcctccta ccagacccag 6180
acaaactccc caaggagagc acggtctgtg gccagccagt ccatcatcgc ctataccatg 6240
agcctgggcg ccgagaattc cgtggcctac tccaacaatt ctatcgccat ccctaccaac 6300
ttcacaatct ccgtgaccac agagatcctg ccagtgagca tgaccaagac atccgtggac 6360
tgcacaatgt atatctgtgg cgattccacc gagtgctcta acctgctgct gcagtacggc 6420
tctttttgta cccagctgaa tagagccctg acaggcatcg ccgtggagca ggacaagaac 6480
acacaggagg tgttcgccca ggtgaagcaa atctacaaga ccccacccat caaggacttt 6540
ggcggcttca acttcagcca gatcctgccc gatcctagca agccatccaa gcggtctttt 6600
atcgaggacc tgctgttcaa caaggtgacc ctggccgatg ccggcttcat caagcagtat 6660
ggcgattgcc tgggcgacat cgccgccaga gacctgatct gtgcccagaa gtttaatggc 6720
ctgaccgtgc tgcctccact gctgacagat gagatgatcg cccagtacac atctgccctg 6780
ctggccggaa ccatcacaag cggatggacc ttcggcgcag gagccgccct gcagatcccc 6840
tttgccatgc agatggccta tcggttcaac ggcatcggcg tgacccagaa tgtgctgtac 6900
gagaaccaga agctgatcgc caatcagttt aactccgcca tcggcaagat ccaggactct 6960
ctgagctcca cagccagcgc cctgggcaag ctgcaggatg tggtgaatca gaacgcccag 7020
gccctgaata ccctggtgaa gcagctgtct agcaacttcg gcgccatctc ctctgtgctg 7080
aatgacatcc tgagccggct ggacaaggtg gaggcagagg tgcagatcga ccggctgatc 7140
acaggcagac tgcagtccct gcagacctac gtgacacagc agctgatcag ggcagcagag 7200
atcagggcct ctgccaatct ggccgccacc aagatgagcg agtgcgtgct gggccagtcc 7260
aagagagtgg acttttgtgg caagggctat cacctgatga gcttcccaca gtccgcccct 7320
cacggagtgg tgtttctgca cgtgacctac gtgccagccc aggagaagaa cttcaccaca 7380
gcaccagcaa tctgccacga tggcaaggca cactttccta gggagggcgt gttcgtgagc 7440
aacggcaccc actggtttgt gacacagcgc aatttctacg agccacagat catcaccaca 7500
gacaatacat tcgtgtccgg caactgtgac gtggtcatcg gcatcgtgaa caataccgtg 7560
tatgatcctc tgcagccaga gctggactct tttaaggagg agctggataa gtacttcaag 7620
aatcacacca gccccgacgt ggatctgggc gacatctctg gcatcaatgc cagcgtggtg 7680
aacatccaga aggagatcga caggctgaac gaggtggcca agaatctgaa cgagtccctg 7740
atcgatctgc aggagctggg caagtatgag cagtacatca agtggccctg gtatatctgg 7800
ctgggcttca tcgccggcct gatcgccatc gtgatggtga ccatcatgct gtgctgtatg 7860
acaagctgct gttcctgcct gaagggctgc tgttcttgtg gcagctgctg taagtttgat 7920
gaggacgata gcgagcctgt gctgaagggc gtgaagctgc actacacctg atagctagcg 7980
atcgcccacc tagtacaacc taaatccatt ataaaaaact taggagcaaa gtgattgcct 8040
cccaaggtcc acaatgacag agacctacga cttcgacaag tcggcatggg acatcaaagg 8100
gtcgatcgct ccgatacaac ccaccaccta cagtgatggc aggctggtgc cccaggtcag 8160
agtcatagat cctggtctag gcgacaggaa ggatgaatgc tttatgtaca tgtttctgct 8220
gggggttgtt gaggacagcg attccctagg gcctccaatc gggcgagcat ttgggttcct 8280
gcccttaggt gttggcagat ccacagcaaa gcccgaaaaa ctcctcaaag aggccactga 8340
gcttgacata gttgttagac gtacagcagg gctcaatgaa aaactggtgt tctacaacaa 8400
caccccacta actctcctca caccttggag aaaggtccta acaacaggga gtgtcttcaa 8460
cgcaaaccaa gtgtgcaatg cggttaatct gataccgctc gataccccgc agaggttccg 8520
tgttgtttat atgagcatca cccgtctttc ggataacggg tattacaccg ttcctagaag 8580
aatgctggaa ttcagatcgg tcaatgcagt ggccttcaac ctgctggtga cccttaggat 8640
tgacaaggcg ataggccctg ggaagatcat cgacaataca gagcaacttc ctgaggcaac 8700
atttatggtc cacatcggga acttcaggag aaagaagagt gaagtctact ctgccgatta 8760
ttgcaaaatg aaaatcgaaa agatgggcct ggtttttgca cttggtggga tagggggcac 8820
cagtcttcac attagaagca caggcaaaat gagcaagact ctccatgcac aactcgggtt 8880
caagaagacc ttatgttacc cgctgatgga tatcaatgaa gaccttaatc gattactctg 8940
gaggagcaga tgcaagatag taagaatcca ggcagttttg cagccatcag ttcctcaaga 9000
attccgcatt tacgacgacg tgatcataaa tgatgaccaa ggactattca aagttctgta 9060
gaccgtagtg cccagcaatg cccgaaaacg acccccctca caatgacagc cagaaggccc 9120
ggacaaaaaa gccccctccg aaagactcca cggaccaagc gagaggccag ccagcagccg 9180
acggcaagcg cgaacaccag gcggccccag cacagaacag ccctgacaca aggccaccac 9240
cagccacccc aatctgcatc ctcctcgtgg gacccccgag gaccaacccc caaggctgcc 9300
cccgatccaa accaccaacc gcatccccac cacccccggg aaagaaaccc ccagcaattg 9360
gaaggcccct ccccctcttc ctcaacacaa gaactccaca accgaaccgc acaagcgacc 9420
gaggtgaccc aaccgcaggc atccgactcc ctagacagat cctctctccc cggcaaacta 9480
aacaaaactt agggccaagg aacatacaca cccaacagaa cccagacccc ggcccacggc 9540
gccgcgcccc caacccccga caaccagagg gagcccccaa ccaatcccgc cggctccccc 9600
ggtgcccaca ggcagggaca ccaacccccg aacagaccca gcacccaacc atcgacaatc 9660
caagacgggg gggccccccc aaaaaaaggc ccccaggggc cgacagccag caccgcgagg 9720
aagcccaccc accccacaca cgaccacggc aaccaaacca gaacccagac caccctgggc 9780
caccagctcc cagactcggc catcaccccg cagaaaggaa aggccacaac ccgcgcaccc 9840
cagccccgat ccggcgggga gccacccaac ccgaaccagc acccaagagc gatccccgaa 9900
ggacccccga accgcaaagg acatcagtat cccacagcct ctccaagtcc cccggtctcc 9960
tcctcttctc gaagggacca aaagatcaat ccaccacacc cgacgacact caactcccca 10020
cccctaaagg agacaccggg aatcccagaa tcaagactca tccaatgtcc atcatgggtc 10080
tcaaggtgaa cgtctctgcc atattcatgg cagtactgtt aactctccaa acacccaccg 10140
gtcaaatcca ttggggcaat ctctctaaga taggggtggt aggaatagga agtgcaagct 10200
acaaagttat gactcgttcc agccatcaat cattagtcat aaaattaatg cccaatataa 10260
ctctcctcaa taactgcacg agggtagaga ttgcagaata caggagacta ctgagaacag 10320
ttttggaacc aattagagat gcacttaatg caatgaccca gaatataaga ccggttcaga 10380
gtgtagcttc aagtaggaga cacaagagat ttgcgggagt agtcctggca ggtgcggccc 10440
taggcgttgc cacagctgct cagataacag ccggcattgc acttcaccag tccatgctga 10500
actctcaagc catcgacaat ctgagagcga gcctggaaac tactaatcag gcaattgaga 10560
caatcagaca agcagggcag gagatgatat tggctgttca gggtgtccaa gactacatca 10620
ataatgagct gataccgtct atgaaccaac tatcttgtga tttaatcggc cagaagctcg 10680
ggctcaaatt gctcagatac tatacagaaa tcctgtcatt atttggcccc agtttacggg 10740
accccatatc tgcggagata tctatccagg ctttgagcta tgcgcttgga ggagacatca 10800
ataaggtgtt agaaaagctc ggatacagtg gaggtgattt actgggcatc ttagagagcg 10860
gaggaataaa ggcccggata actcacgtcg acacagagtc ctacttcatt gtcctcagta 10920
tagcctatcc gacgctgtcc gagattaagg gggtgattgt ccaccggcta gagggggtct 10980
cgtacaacat aggctctcaa gagtggtata ccactgtgcc caagtatgtt gcaacccaag 11040
ggtaccttat ctcgaatttt gatgagtcat cgtgtacttt catgccagag gggactgtgt 11100
gcagccaaaa tgccttgtac ccgatgagtc ctctgctcca agaatgcctc cgggggtaca 11160
ccaagtcctg tgctcgtaca ctcgtatccg ggtcttttgg gaaccggttc attttatcac 11220
aagggaacct aatagccaat tgtgcatcaa tcctttgcaa gtgttacaca acaggaacga 11280
tcattaatca agaccctgac aagatcctaa catacattgc tgccgatcac tgcccggtag 11340
tcgaggtgaa cggcgtgacc atccaagtcg ggagcaggag gtatccagac gctgtgtact 11400
tgcacagaat tgacctcggt cctcccatat cattggagag gttggacgta gggacaaatc 11460
tggggaatgc aattgctaag ttggaggatg ccaaggaatt gttggagtca tcggaccaga 11520
tattgaggag tatgaaaggt ttatcgagca ctagcatagt ctacatcctg attgcagtgt 11580
gtcttggagg gttgataggg atccccgctt taatatgttg ctgcaggggg cgttgtaaca 11640
aaaagggaga acaagttggt atgtcaagac caggcctaaa gcctgatctt acgggaacat 11700
caaaatccta tgtaaggtcg ctctgatcct ctacaactct tgaaacacaa atgtcccaca 11760
agtctcctct tcgtcatcaa gcaaccaccg cacccagcat caagcccacc tgaaattatc 11820
tccggcttcc ctctggccga acaatatcgg tagttaatta aaacttaggg tgcaagatca 11880
tcgataatgt caccacaacg agaccggata aatgccttct acaaagataa cccccatccc 11940
aagggaagta ggatagtcat taacagagaa catcttatga ttgatagacc ttatgttttg 12000
ctggctgttc tgtttgtcat gtttctgagc ttgatcgggt tgctagccat tgcaggaatt 12060
cgacttcatc gggcagccat ctacaccgca gagatccata aaagcctcag caccaatcta 12120
gatgtaacta actcaatcga gcatcaggtc aaggacgtgc tgacaccact cttcaaaatc 12180
atcggtgatg aagtgggcct gaggacacct cagagattca ctgacctagt gaaattaatc 12240
tctgacaaga ttaaattcct taatccggat agggagtacg acttcagaga tctcacttgg 12300
tgtatcaacc cgccagagag aatcaaattg gattatgatc aatactgtgc agatgtggct 12360
gctgaagagc tcatgaatgc attggtgaac tcaactctac tggagaccag aacaaccaat 12420
cagttcctag ctgtctcaaa gggaaactgc tcagggccca ctacaatcag aggtcaattc 12480
tcaaacatgt cgctgtccct gttagacttg tatttaggtc gaggttacaa tgtgtcatct 12540
atagtcacta tgacatccca gggaatgtat gggggaactt acctagtgga aaagcctaat 12600
ctgagcagca aaaggtcaga gttgtcacaa ctgagcatgt accgagtgtt tgaagtaggt 12660
gttatcagaa atccgggttt gggggctccg gtgttccata tgacaaacta tcttgagcaa 12720
ccagtcagta atgatctcag caactgtatg gtggctttgg gggagctcaa actcgcagcc 12780
ctttgtcacg gggaagattc tatcacaatt ccctatcagg gatcagggaa aggtgtcagc 12840
ttccagctcg tcaagctagg tgtctggaaa tccccaaccg acatgcaatc ctgggtcccc 12900
ttatcaacgg atgatccagt gatagacagg ctttacctct catctcacag aggtgttatc 12960
gctgacaacc aagcaaaatg ggctgtcccg acaacacgaa cagatgacaa gttgcgaatg 13020
gagacatgct tccaacaggc gtgtaagggt aaaatccaag cactctgcga gaatcccgag 13080
tgggcaccat tgaaggataa caggattcct tcatacgggg tcttgtctgt tgatctgagt 13140
ctgacagttg agcttaaaat caaaattgct tcgggattcg ggccattgat cacacacggt 13200
tcagggatgg acctatacaa atccaaccac aacaatgtgt attggctgac tatcccgcca 13260
atgaagaacc tagccttagg tgtaatcaac acattggagt ggataccgag attcaaggtt 13320
agtccctacc tcttcactgt cccaattaag gaagcaggcg aagactgcca tgccccaaca 13380
tacctacctg cggaggtgga tggtgatgtc aaactcagtt ccaatctggt gattctacct 13440
ggtcaagatc tccaatatgt tttggcaacc tacgatactt ccagggttga acatgctgtg 13500
gtttattacg tttacagccc aagccgctca ttttcttact tttatccttt taggttgcct 13560
ataaaggggg tccccatcga attacaagtg gaatgcttca catgggacca aaaactctgg 13620
tgccgtcact tctgtgtgct tgcggactca gaatctggtg gacatatcac tcactctggg 13680
atggtgggca tgggagtcag ctgcacagtc acccgggaag atggaaccaa tcgcagatag 13740
ggctgctagt gaaccaatca catgatgtca cccagacatc aggcataccc actagtgtga 13800
aatagacatc agaattaaga aaaacgtagg gtccaagtgg ttccccgtta tggactcgct 13860
atctgtcaac cagatcttat accctgaagt tcacctagat agcccgatag ttaccaataa 13920
gatagtagcc atcctggagt atgctcgagt ccctcacgct tacagcctgg aggaccctac 13980
actgtgtcag aacatcaagc accgcctaaa aaacggattt tccaaccaaa tgattataaa 14040
caatgtggaa gttgggaatg tcatcaagtc caagcttagg agttatccgg cccactctca 14100
tattccatat ccaaattgta atcaggattt atttaacata gaagacaaag agtcaacgag 14160
gaagatccgt gaactcctca aaaaggggaa ttcgctgtac tccaaagtca gtgataaggt 14220
tttccaatgc ttaagggaca ctaactcacg gcttggccta ggctccgaat tgagggagga 14280
catcaaggag aaagttatta acttgggagt ttacatgcac agctcccagt ggtttgagcc 14340
ctttctgttt tggtttacag tcaagactga gatgaggtca gtgattaaat cacaaaccca 14400
tacttgccat aggaggagac acacacctgt attcttcact ggtagttcag ttgagttgct 14460
aatctctcgt gaccttgttg ctataatcag taaagagtct caacatgtat attacctgac 14520
atttgaactg gttttgatgt attgtgatgt catagagggg aggttaatga cagagaccgc 14580
tatgactatt gatgctaggt atacagagct tctaggaaga gtcagataca tgtggaaact 14640
gatagatggt ttcttccctg cactcgggaa tccaacttat caaattgtag ccatgctgga 14700
gcctctttca cttgcttacc tgcagctgag ggatataaca gtagaactca gaggtgcttt 14760
ccttaaccac tgctttactg aaatacatga tgttcttgac caaaacgggt tttctgatga 14820
aggtacttat catgagttaa ctgaagctct agattacatt ttcataactg atgacataca 14880
tctgacaggg gagattttct catttttcag aagtttcggc caccccagac ttgaagcagt 14940
aacggctgct gaaaatgtta ggaaatacat gaatcagcct aaagtcattg tgtatgagac 15000
tctgatgaaa ggtcatgcca tattttgtgg aatcataatc aacggctatc gtgacaggca 15060
cggaggcagt tggccaccgc tgaccctccc cctgcatgct gcagacacaa tccggaatgc 15120
tcaagcttca ggtgaagggt taacacatga gcagtgcgtt gataactgga aatcttttgc 15180
tggagtgaaa tttggctgct ttatgcctct tagcctggat agtgatctga caatgtacct 15240
aaaggacaag gcacttgctg ctctccaaag ggaatgggat tcagtttacc cgaaagagtt 15300
cctgcgttac gaccctccca agggaaccgg gtcacggagg cttgtagatg ttttccttaa 15360
tgattcgagc tttgacccat atgatgtgat aatgtatgtt gtaagtggag cttacctcca 15420
tgaccctgag ttcaacctgt cttacagcct gaaagaaaag gagatcaagg aaacaggtag 15480
actttttgct aaaatgactt acaaaatgag ggcatgccaa gtgattgctg aaaatctaat 15540
ctcaaacggg attggcaaat attttaagga caatgggatg gccaaggatg agcacgattt 15600
gactaaggca ctccacactc tagctgtctc aggagtcccc aaagatctca aagaaagtca 15660
cagggggggg ccagtcttaa aaacctactc ccgaagccca gtccacacaa gtaccaggaa 15720
cgtgagagca gcaaaagggt ttatagggtt ccctcaagta attcggcagg accaagacac 15780
tgatcatccg gagaatatgg aagcttacga gacagtcagt gcatttatca cgactgatct 15840
caagaagtac tgccttaatt ggagatatga gaccatcagc ttgtttgcac agaggctaaa 15900
tgagatttac ggattgccct catttttcca gtggctgcat aagaggcttg agacctctgt 15960
cctgtatgta agtgaccctc attgcccccc cgaccttgac gcccatatcc cgttatataa 16020
agtccccaat gatcaaatct tcattaagta ccctatggga ggtatagaag ggtattgtca 16080
gaagctgtgg accatcagca ccattcccta tctatacctg gctgcttatg agagcggagt 16140
aaggattgct tcgttagtgc aaggggacaa tcagaccata gccgtaacaa aaagggtacc 16200
cagcacatgg ccctacaacc ttaagaaacg ggaagctgct agagtaacta gagattactt 16260
tgtaattctt aggcaaaggc tacatgatat tggccatcac ctcaaggcaa atgagacaat 16320
tgtttcatca catttttttg tctattcaaa aggaatatat tatgatgggc tacttgtgtc 16380
ccaatcactc aagagcatcg caagatgtgt attctggtca gagactatag ttgatgaaac 16440
aagggcagca tgcagtaata ttgctacaac aatggctaaa agcatcgaga gaggttatga 16500
ccgttacctt gcatattccc tgaacgtcct aaaagtgata cagcaaattc tgatctctct 16560
tggcttcaca atcaattcaa ccatgacccg ggatgtagtc atacccctcc tcacaaacaa 16620
cgacctctta ataaggatgg cactgttgcc cgctcctatt ggggggatga attatctgaa 16680
tatgagcagg ctgtttgtca gaaacatcgg tgatccagta acatcatcaa ttgctgatct 16740
caagagaatg attctcgcct cactaatgcc tgaagagacc ctccatcagg taatgacaca 16800
acaaccgggg gactcttcat tcctagactg ggctagcgac ccttactcag caaatcttgt 16860
atgtgtccag agcatcacta gactcctcaa gaacataact gcaaggtttg tcctgatcca 16920
tagtccaaac ccaatgttaa aaggattatt ccatgatgac agtaaagaag aggacgaggg 16980
actggcggca ttcctcatgg acaggcatat tatagtacct agggcagctc atgaaatcct 17040
ggatcatagt gtcacagggg caagagagtc tattgcaggc atgctggata ccacaaaagg 17100
cttgattcga gccagcatga ggaagggggg tttaacctct cgagtgataa ccagattgtc 17160
caattatgac tatgaacaat tcagagcagg gatggtgcta ttgacaggaa gaaagagaaa 17220
tgtcctcatt gacaaagagt catgttcagt gcagctggcg agagctctaa gaagccatat 17280
gtgggcgagg ctagctcgag gacggcctat ttacggcctt gaggtccctg atgtactaga 17340
atctatgcga ggccacctta ttcggcgtca tgagacatgt gtcatctgcg agtgtggatc 17400
agtcaactac ggatggtttt ttgtcccctc gggttgccaa ctggatgata ttgacaagga 17460
aacatcatcc ttgagagtcc catatattgg ttctaccact gatgagagaa cagacatgaa 17520
gcttgccttc gtaagagccc caagtcgatc cttgcgatct gctgttagaa tagcaacagt 17580
gtactcatgg gcttacggtg atgatgatag ctcttggaac gaagcctggt tgttggctag 17640
gcaaagggcc aatgtgagcc tggaggagct aagggtgatc actcccatct caacttcgac 17700
taatttagcg cataggttga gggatcgtag cactcaagtg aaatactcag gtacatccct 17760
tgtccgagtg gcgaggtata ccacaatctc caacgacaat ctctcatttg tcatatcaga 17820
taagaaggtt gatactaact ttatatacca acaaggaatg cttctagggt tgggtgtttt 17880
agaaacattg tttcgactcg agaaagatac cggatcatct aacacggtat tacatcttca 17940
cgtcgaaaca gattgttgcg tgatcccgat gatagatcat cccaggatac ccagctcccg 18000
caagctagag ctgagggcag agctatgtac caacccattg atatatgata atgcaccttt 18060
aattgacaga gatgcaacaa ggctatacac ccagagccat aggaggcacc ttgtggaatt 18120
tgttacatgg tccacacccc aactatatca cattttagct aagtccacag cactatctat 18180
gattgacctg gtaacaaaat ttgagaagga ccatatgaat gaaatttcag ctctcatagg 18240
ggatgacgat atcaatagtt tcataactga gtttctgctc atagagccaa gattattcac 18300
tatctacttg ggccagtgtg cggccatcaa ttgggcattt gatgtacatt atcatagacc 18360
atcagggaaa tatcagatgg gtgagctgtt gtcatcgttc ctttctagaa tgagcaaagg 18420
agtgtttaag gtgcttgtca atgctctaag ccacccaaag atctacaaga aattctggca 18480
ttgtggtatt atagagccta tccatggtcc ttcacttgat gctcaaaact tgcacacaac 18540
tgtgtgcaac atggtttaca catgctatat gacctacctc gacctgttgt tgaatgaaga 18600
gttagaagag ttcacatttc tcttgtgtga aagcgacgag gatgtagtac cggacagatt 18660
cgacaacatc caggcaaaac acttatgtgt tctggcagat ttgtactgtc aaccagggac 18720
ctgcccacca attcgaggtc taagaccggt agagaaatgt gcagttctaa ccgaccatat 18780
caaggcagag gctatgttat ctccagcagg atcttcgtgg aacataaatc caattattgt 18840
agaccattac tcatgctccc tgacttatct ccggcgagga tcgatcaaac agataagatt 18900
gagagttgat ccaggattca ttttcgacgc cctcgctgag gtaaatgtca gtcagccaaa 18960
gatcggcagc aacaacatct caaatatgag catcaaggct ttcagacccc cacacgatga 19020
tgttgcaaaa ttgctcaaag atatcaacac aagcaagcac aatcttccca tttcaggggg 19080
caatctcgcc aattatgaaa tccatgcttt ccgcagaatc gggttgaact catctgcttg 19140
ctacaaagct gttgagatat caacattaat taggagatgc cttgagccag gggaggacgg 19200
cttgttcttg ggtgagggat cgggttctat gttgatcact tataaggaga tacttaaact 19260
aaacaagtgc ttctataata gtggggtttc cgccaattct agatctggtc aaagggaatt 19320
agcaccctat ccctccgaag ttggccttgt cgaacacaga atgggagtag gtaatattgt 19380
caaagtgctc tttaacggga ggcccgaagt cacgtgggta ggcagtgtag attgcttcaa 19440
tttcatagtt agtaatatcc ctacctctag tgtggggttt atccattcag atatagagac 19500
cttgcctgac aaagatacta tagagaagct agaggaattg gcagccatct tatcgatggc 19560
tctgctcctg ggcaaaatag gatcaatact ggtgattaag cttatgcctt tcagcgggga 19620
ttttgttcag ggatttataa gttatgtagg gtctcattat agagaagtga accttgtata 19680
ccctagatac agcaacttca tatctactga atcttatttg gttatgacag atctcaaggc 19740
taaccggcta atgaatcctg aaaagattaa gcagcagata attgaatcat ctgtgaggac 19800
ttcacctgga cttataggtc acatcctatc cattaagcaa ctaagctgca tacaagcaat 19860
tgtgggagac gcagttagta gaggtgatat caatcctact ctgaaaaaac ttacacctat 19920
agagcaggtg ctgatcaatt gcgggttggc aattaacgga cctaagctgt gcaaagaatt 19980
gatccaccat gatgttgcct cagggcaaga tggattgctt aattctatac tcatcctcta 20040
cagggagttg gcaagattca aagacaacca aagaagtcaa caagggatgt tccacgccta 20100
ccccgtattg gtaagtagca ggcaacgaga acttatatct aggatcaccc gcaaattttg 20160
ggggcacatt cttctttact ccgggaacaa aaagttgata aataagttta tccagaatct 20220
caagtccggc tatctgatac tagacttaca ccagaatatc ttcgttaaga atctatccaa 20280
gtcagagaaa cagattatta tgacgggggg tttgaaacgt gagtgggttt ttaaggtaac 20340
agtcaaggag accaaagaat ggtataagtt agtcggatac agtgccctga ttaaggacta 20400
attggttgaa ctccggaacc ctaatcctgc cctaggtggt taggcattat ttgcaatata 20460
ttaaagaaaa ctttgaaaat acgaagtttc tattcccagc tttgtctggt ggccggcata 20520
gtcccagcct cctcgctggc gctggctggg caacattccg aggggaccgt ccccacggta 20580
atggcgaatg ggacgcggcc gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg 20640
ctgctggcgc tggctgggca ataactagca taaccccttg gggcctctaa acgggtcttg 20700
aggggttttt tgctgaaagg aggaactata tccggatgcg gccgcgcgct tggcgtaatc 20760
atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 20820
agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 20880
tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 20940
aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 21000
cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 21060
ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 21120
ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 21180
cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 21240
actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 21300
cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 21360
tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 21420
gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 21480
caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 21540
agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 21600
tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 21660
tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 21720
gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 21780
gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 21840
aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 21900
atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 21960
gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 22020
acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 22080
ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 22140
tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 22200
ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 22260
ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 22320
atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 22380
taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 22440
catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 22500
atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 22560
acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 22620
aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 22680
ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 22740
cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 22800
atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 22860
ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctg 22916
<210> 5
<211> 22916
<212> DNA
<213> 人工序列
<220>
<223> MV WuhanCoV S 在第6位
<400> 5
ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 60
cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 120
cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 180
tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 240
gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 300
tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 360
ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 420
taacgcgaat tttaacaaaa tattaacgtt tacaatttcg cgccattcgc cattcaggct 480
gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 540
agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 600
ttgtaaaacg acggccagtg cgcgccgtta atacgactca ctatagggag acccaagctg 660
gctagctttg tttggtctga tgagtcccgt gaggacgaaa cccggcgtac cgggtcacca 720
aacaaagttg ggtaaggata gttcaatcaa tgatcatctt ctagtgcact taggattcaa 780
gatcctatta tcagggacaa gagcaggatt agggatatcc gagatggcca cacttttaag 840
gagcttagca ttgttcaaaa gaaacaagga caaaccaccc attacatcag gatccggtgg 900
agccatcaga ggaatcaaac acattattat agtaccaatc cctggagatt cctcaattac 960
cactcgatcc agacttctgg accggttggt gaggttaatt ggaaacccgg atgtgagcgg 1020
gcccaaacta acaggggcac taataggtat attatcctta tttgtggagt ctccaggtca 1080
attgattcag aggatcaccg atgaccctga cgttagcata aggctgttag aggttgtcca 1140
gagtgaccag tcacaatctg gccttacctt cgcatcaaga ggtaccaaca tggaggatga 1200
ggcggaccaa tacttttcac atgatgatcc aattagtagt gatcaatcca ggttcggatg 1260
gttcgggaac aaggaaatct cagatattga agtgcaagac cctgagggat tcaacatgat 1320
tctgggtacc atcctagccc aaatttgggt cttgctcgca aaggcggtta cggccccaga 1380
cacggcagct gattcggagc taagaaggtg gataaagtac acccaacaaa gaagggtagt 1440
tggtgaattt agattggaga gaaaatggtt ggatgtggtg aggaacagga ttgccgagga 1500
cctctcctta cgccgattca tggtcgctct aatcctggat atcaagagaa cacccggaaa 1560
caaacccagg attgctgaaa tgatatgtga cattgataca tatatcgtag aggcaggatt 1620
agccagtttt atcctgacta ttaagtttgg gatagaaact atgtatcctg ctcttggact 1680
gcatgaattt gctggtgagt tatccacact tgagtccttg atgaaccttt accagcaaat 1740
gggggaaact gcaccctaca tggtaatcct ggagaactca attcagaaca agttcagtgc 1800
aggatcatac cctctgctct ggagctatgc catgggagta ggagtggaac ttgaaaactc 1860
catgggaggt ttgaactttg gccgatctta ctttgatcca gcatatttta gattagggca 1920
agagatggta aggaggtcag ctggaaaggt cagttccaca ttggcatctg aactcggtat 1980
cactgccgag gatgcaaggc ttgtttcaga gattgcaatg catactactg aggacaagat 2040
cagtagagcg gttggaccca gacaagccca agtatcattt ctacacggtg atcaaagtga 2100
gaatgagcta ccgagattgg ggggcaagga agataggagg gtcaaacaga gtcgaggaga 2160
agccagggag agctacagag aaaccgggcc cagcagagca agtgatgcga gagctgccca 2220
tcttccaacc ggcacacccc tagacattga cactgcaacg gagtccagcc aagatccgca 2280
ggacagtcga aggtcagctg acgccctgct taggctgcaa gccatggcag gaatctcgga 2340
agaacaaggc tcagacacgg acacccctat agtgtacaat gacagaaatc ttctagacta 2400
ggtgcgagag gccgagggcc agaacaacat ccgcctacca tccatcattg ttataaaaaa 2460
cttaggaacc aggtccacac agccgccagc ccatcaacca tccactccca cgattggagc 2520
caatggcaga agagcaggca cgccatgtca aaaacggact ggaatgcatc cgggctctca 2580
aggccgagcc catcggctca ctggccatcg aggaagctat ggcagcatgg tcagaaatat 2640
cagacaaccc aggacaggag cgagccacct gcagggaaga gaaggcaggc agttcgggtc 2700
tcagcaaacc atgcctctca gcaattggat caactgaagg cggtgcacct cgcatccgcg 2760
gtcagggacc tggagagagc gatgacgacg ctgaaacttt gggaatcccc ccaagaaatc 2820
tccaggcatc aagcactggg ttacagtgtt attacgttta tgatcacagc ggtgaagcgg 2880
ttaagggaat ccaagatgct gactctatca tggttcaatc aggccttgat ggtgatagca 2940
ccctctcagg aggagacaat gaatctgaaa acagcgatgt ggatattggc gaacctgata 3000
ccgagggata tgctatcact gaccggggat ctgctcccat ctctatgggg ttcagggctt 3060
ctgatgttga aactgcagaa ggaggggaga tccacgagct cctgagactc caatccagag 3120
gcaacaactt tccgaagctt gggaaaactc tcaatgttcc tccgcccccg gaccccggta 3180
gggccagcac ttccgggaca cccattaaaa agggcacaga cgcgagatta gcctcatttg 3240
gaacggagat cgcgtcttta ttgacaggtg gtgcaaccca atgtgctcga aagtcaccct 3300
cggaaccatc agggccaggt gcacctgcgg ggaatgtccc cgagtgtgtg agcaatgccg 3360
cactgataca ggagtggaca cccgaatctg gtaccacaat ctccccgaga tcccagaata 3420
atgaagaagg gggagactat tatgatgatg agctgttctc tgatgtccaa gatattaaaa 3480
cagccttggc caaaatacac gaggataatc agaagataat ctccaagcta gaatcactgc 3540
tgttattgaa gggagaagtt gagtcaatta agaagcagat caacaggcaa aatatcagca 3600
tatccaccct ggaaggacac ctctcaagca tcatgatcgc cattcctgga cttgggaagg 3660
atcccaacga ccccactgca gatgtcgaaa tcaatcccga cttgaaaccc atcataggca 3720
gagattcagg ccgagcactg gccgaagttc tcaagaaacc cgttgccagc cgacaactcc 3780
aaggaatgac aaatggacgg accagttcca gaggacagct gctgaaggaa tttcagctaa 3840
agccgatcgg gaaaaagatg agctcagccg tcgggtttgt tcctgacacc ggccctgcat 3900
cacgcagtgt aatccgctcc attataaaat ccagccggct agaggaggat cggaagcgtt 3960
acctgatgac tctccttgat gatatcaaag gagccaatga tcttgccaag ttccaccaga 4020
tgctgatgaa gataataatg aagtagctac agctcaactt acctgccaac cccatgccag 4080
tcgacccacc tagtacaacc taaatccatt ataaaaaact taggagcaaa gtgattgcct 4140
cccaaggtcc acaatgacag agacctacga cttcgacaag tcggcatggg acatcaaagg 4200
gtcgatcgct ccgatacaac ccaccaccta cagtgatggc aggctggtgc cccaggtcag 4260
agtcatagat cctggtctag gcgacaggaa ggatgaatgc tttatgtaca tgtttctgct 4320
gggggttgtt gaggacagcg attccctagg gcctccaatc gggcgagcat ttgggttcct 4380
gcccttaggt gttggcagat ccacagcaaa gcccgaaaaa ctcctcaaag aggccactga 4440
gcttgacata gttgttagac gtacagcagg gctcaatgaa aaactggtgt tctacaacaa 4500
caccccacta actctcctca caccttggag aaaggtccta acaacaggga gtgtcttcaa 4560
cgcaaaccaa gtgtgcaatg cggttaatct gataccgctc gataccccgc agaggttccg 4620
tgttgtttat atgagcatca cccgtctttc ggataacggg tattacaccg ttcctagaag 4680
aatgctggaa ttcagatcgg tcaatgcagt ggccttcaac ctgctggtga cccttaggat 4740
tgacaaggcg ataggccctg ggaagatcat cgacaataca gagcaacttc ctgaggcaac 4800
atttatggtc cacatcggga acttcaggag aaagaagagt gaagtctact ctgccgatta 4860
ttgcaaaatg aaaatcgaaa agatgggcct ggtttttgca cttggtggga tagggggcac 4920
cagtcttcac attagaagca caggcaaaat gagcaagact ctccatgcac aactcgggtt 4980
caagaagacc ttatgttacc cgctgatgga tatcaatgaa gaccttaatc gattactctg 5040
gaggagcaga tgcaagatag taagaatcca ggcagttttg cagccatcag ttcctcaaga 5100
attccgcatt tacgacgacg tgatcataaa tgatgaccaa ggactattca aagttctgta 5160
gaccgtagtg cccagcaatg cccgaaaacg acccccctca caatgacagc cagaaggccc 5220
ggacaaaaaa gccccctccg aaagactcca cggaccaagc gagaggccag ccagcagccg 5280
acggcaagcg cgaacaccag gcggccccag cacagaacag ccctgacaca aggccaccac 5340
cagccacccc aatctgcatc ctcctcgtgg gacccccgag gaccaacccc caaggctgcc 5400
cccgatccaa accaccaacc gcatccccac cacccccggg aaagaaaccc ccagcaattg 5460
gaaggcccct ccccctcttc ctcaacacaa gaactccaca accgaaccgc acaagcgacc 5520
gaggtgaccc aaccgcaggc atccgactcc ctagacagat cctctctccc cggcaaacta 5580
aacaaaactt agggccaagg aacatacaca cccaacagaa cccagacccc ggcccacggc 5640
gccgcgcccc caacccccga caaccagagg gagcccccaa ccaatcccgc cggctccccc 5700
ggtgcccaca ggcagggaca ccaacccccg aacagaccca gcacccaacc atcgacaatc 5760
caagacgggg gggccccccc aaaaaaaggc ccccaggggc cgacagccag caccgcgagg 5820
aagcccaccc accccacaca cgaccacggc aaccaaacca gaacccagac caccctgggc 5880
caccagctcc cagactcggc catcaccccg cagaaaggaa aggccacaac ccgcgcaccc 5940
cagccccgat ccggcgggga gccacccaac ccgaaccagc acccaagagc gatccccgaa 6000
ggacccccga accgcaaagg acatcagtat cccacagcct ctccaagtcc cccggtctcc 6060
tcctcttctc gaagggacca aaagatcaat ccaccacacc cgacgacact caactcccca 6120
cccctaaagg agacaccggg aatcccagaa tcaagactca tccaatgtcc atcatgggtc 6180
tcaaggtgaa cgtctctgcc atattcatgg cagtactgtt aactctccaa acacccaccg 6240
gtcaaatcca ttggggcaat ctctctaaga taggggtggt aggaatagga agtgcaagct 6300
acaaagttat gactcgttcc agccatcaat cattagtcat aaaattaatg cccaatataa 6360
ctctcctcaa taactgcacg agggtagaga ttgcagaata caggagacta ctgagaacag 6420
ttttggaacc aattagagat gcacttaatg caatgaccca gaatataaga ccggttcaga 6480
gtgtagcttc aagtaggaga cacaagagat ttgcgggagt agtcctggca ggtgcggccc 6540
taggcgttgc cacagctgct cagataacag ccggcattgc acttcaccag tccatgctga 6600
actctcaagc catcgacaat ctgagagcga gcctggaaac tactaatcag gcaattgaga 6660
caatcagaca agcagggcag gagatgatat tggctgttca gggtgtccaa gactacatca 6720
ataatgagct gataccgtct atgaaccaac tatcttgtga tttaatcggc cagaagctcg 6780
ggctcaaatt gctcagatac tatacagaaa tcctgtcatt atttggcccc agtttacggg 6840
accccatatc tgcggagata tctatccagg ctttgagcta tgcgcttgga ggagacatca 6900
ataaggtgtt agaaaagctc ggatacagtg gaggtgattt actgggcatc ttagagagcg 6960
gaggaataaa ggcccggata actcacgtcg acacagagtc ctacttcatt gtcctcagta 7020
tagcctatcc gacgctgtcc gagattaagg gggtgattgt ccaccggcta gagggggtct 7080
cgtacaacat aggctctcaa gagtggtata ccactgtgcc caagtatgtt gcaacccaag 7140
ggtaccttat ctcgaatttt gatgagtcat cgtgtacttt catgccagag gggactgtgt 7200
gcagccaaaa tgccttgtac ccgatgagtc ctctgctcca agaatgcctc cgggggtaca 7260
ccaagtcctg tgctcgtaca ctcgtatccg ggtcttttgg gaaccggttc attttatcac 7320
aagggaacct aatagccaat tgtgcatcaa tcctttgcaa gtgttacaca acaggaacga 7380
tcattaatca agaccctgac aagatcctaa catacattgc tgccgatcac tgcccggtag 7440
tcgaggtgaa cggcgtgacc atccaagtcg ggagcaggag gtatccagac gctgtgtact 7500
tgcacagaat tgacctcggt cctcccatat cattggagag gttggacgta gggacaaatc 7560
tggggaatgc aattgctaag ttggaggatg ccaaggaatt gttggagtca tcggaccaga 7620
tattgaggag tatgaaaggt ttatcgagca ctagcatagt ctacatcctg attgcagtgt 7680
gtcttggagg gttgataggg atccccgctt taatatgttg ctgcaggggg cgttgtaaca 7740
aaaagggaga acaagttggt atgtcaagac caggcctaaa gcctgatctt acgggaacat 7800
caaaatccta tgtaaggtcg ctctgatcct ctacaactct tgaaacacaa atgtcccaca 7860
agtctcctct tcgtcatcaa gcaaccaccg cacccagcat caagcccacc tgaaattatc 7920
tccggcttcc ctctggccga acaatatcgg tagttaatta aaacttaggg tgcaagatca 7980
tcgataatgt caccacaacg agaccggata aatgccttct acaaagataa cccccatccc 8040
aagggaagta ggatagtcat taacagagaa catcttatga ttgatagacc ttatgttttg 8100
ctggctgttc tgtttgtcat gtttctgagc ttgatcgggt tgctagccat tgcaggaatt 8160
cgacttcatc gggcagccat ctacaccgca gagatccata aaagcctcag caccaatcta 8220
gatgtaacta actcaatcga gcatcaggtc aaggacgtgc tgacaccact cttcaaaatc 8280
atcggtgatg aagtgggcct gaggacacct cagagattca ctgacctagt gaaattaatc 8340
tctgacaaga ttaaattcct taatccggat agggagtacg acttcagaga tctcacttgg 8400
tgtatcaacc cgccagagag aatcaaattg gattatgatc aatactgtgc agatgtggct 8460
gctgaagagc tcatgaatgc attggtgaac tcaactctac tggagaccag aacaaccaat 8520
cagttcctag ctgtctcaaa gggaaactgc tcagggccca ctacaatcag aggtcaattc 8580
tcaaacatgt cgctgtccct gttagacttg tatttaggtc gaggttacaa tgtgtcatct 8640
atagtcacta tgacatccca gggaatgtat gggggaactt acctagtgga aaagcctaat 8700
ctgagcagca aaaggtcaga gttgtcacaa ctgagcatgt accgagtgtt tgaagtaggt 8760
gttatcagaa atccgggttt gggggctccg gtgttccata tgacaaacta tcttgagcaa 8820
ccagtcagta atgatctcag caactgtatg gtggctttgg gggagctcaa actcgcagcc 8880
ctttgtcacg gggaagattc tatcacaatt ccctatcagg gatcagggaa aggtgtcagc 8940
ttccagctcg tcaagctagg tgtctggaaa tccccaaccg acatgcaatc ctgggtcccc 9000
ttatcaacgg atgatccagt gatagacagg ctttacctct catctcacag aggtgttatc 9060
gctgacaacc aagcaaaatg ggctgtcccg acaacacgaa cagatgacaa gttgcgaatg 9120
gagacatgct tccaacaggc gtgtaagggt aaaatccaag cactctgcga gaatcccgag 9180
tgggcaccat tgaaggataa caggattcct tcatacgggg tcttgtctgt tgatctgagt 9240
ctgacagttg agcttaaaat caaaattgct tcgggattcg ggccattgat cacacacggt 9300
tcagggatgg acctatacaa atccaaccac aacaatgtgt attggctgac tatcccgcca 9360
atgaagaacc tagccttagg tgtaatcaac acattggagt ggataccgag attcaaggtt 9420
agtccctacc tcttcactgt cccaattaag gaagcaggcg aagactgcca tgccccaaca 9480
tacctacctg cggaggtgga tggtgatgtc aaactcagtt ccaatctggt gattctacct 9540
ggtcaagatc tccaatatgt tttggcaacc tacgatactt ccagggttga acatgctgtg 9600
gtttattacg tttacagccc aagccgctca ttttcttact tttatccttt taggttgcct 9660
ataaaggggg tccccatcga attacaagtg gaatgcttca catgggacca aaaactctgg 9720
tgccgtcact tctgtgtgct tgcggactca gaatctggtg gacatatcac tcactctggg 9780
atggtgggca tgggagtcag ctgcacagtc acccgggaag atggaaccaa tcgcagatag 9840
ggctgctagt gaaccaatca catgatgtca cccagacatc aggcataccc accatccatc 9900
attgttataa aaaacttagg aaccaggtcc acacagagtg atacgcgtac gccaccatgt 9960
tcgtgtttct ggtgctgctg cctctggtga gctcccagtg cgtgaacctg accacaagga 10020
cccagctgcc ccctgcctat accaattcct tcacacgggg cgtgtactat cccgacaagg 10080
tgttccggag cagcgtgctg cactccacac aggatctgtt tctgcctttc ttttctaacg 10140
tgacctggtt ccacgccatc cacgtgagcg gcaccaatgg cacaaagcgg ttcgacaatc 10200
cagtgctgcc ctttaacgat ggcgtgtact tcgcctccac cgagaagtct aacatcatca 10260
gaggctggat ctttggcacc acactggaca gcaagacaca gtccctgctg atcgtgaaca 10320
atgccaccaa cgtggtcatc aaggtgtgcg agttccagtt ttgtaatgat ccattcctgg 10380
gcgtgtacta tcacaagaac aataagtctt ggatggagag cgagtttcgc gtgtattcct 10440
ctgccaacaa ttgcacattt gagtacgtgt cccagccctt cctgatggac ctggagggca 10500
agcagggcaa tttcaagaac ctgagggagt tcgtgtttaa gaatatcgat ggctacttca 10560
aaatctactc caagcacacc ccaatcaacc tggtgcgcga cctgccacag ggcttctctg 10620
ccctggagcc actggtggat ctgcccatcg gcatcaacat cacccggttt cagacactgc 10680
tggccctgca cagaagctac ctgacaccag gcgacagctc ctctggatgg accgcaggag 10740
cagcagccta ctatgtgggc tatctgcagc ccaggacctt cctgctgaag tacaacgaga 10800
atggcaccat cacagacgcc gtggattgcg ccctggatcc cctgtctgag accaagtgta 10860
cactgaagag ctttaccgtg gagaagggca tctatcagac aagcaatttc agggtgcagc 10920
ctaccgagtc catcgtgcgc tttcccaata tcacaaacct gtgccctttt ggcgaggtgt 10980
tcaacgcaac ccgcttcgcc agcgtgtacg cctggaatag gaagcgcatc tccaactgcg 11040
tggccgacta ttctgtgctg tacaacagcg cctccttctc tacctttaag tgctatggcg 11100
tgagccccac aaagctgaat gacctgtgct ttaccaacgt gtacgccgat tccttcgtga 11160
tcaggggcga cgaggtgcgc cagatcgcac caggacagac aggcaagatc gcagactaca 11220
attataagct gcctgacgat ttcaccggct gcgtgatcgc ctggaactct aacaatctgg 11280
atagcaaagt gggcggcaac tacaattatc tgtaccggct gtttagaaag tctaatctga 11340
agccattcga gagggacatc tccacagaaa tctaccaggc cggctctacc ccctgcaatg 11400
gcgtggaggg ctttaactgt tatttccctc tgcagagcta cggcttccag ccaacaaacg 11460
gcgtgggcta tcagccctac cgcgtggtgg tgctgtcttt tgagctgctg cacgcacctg 11520
caacagtgtg cggaccaaag aagagcacca atctggtgaa gaacaagtgc gtgaacttca 11580
acttcaacgg actgaccgga acaggcgtgc tgaccgagtc caacaagaag ttcctgcctt 11640
ttcagcagtt cggcagggac atcgcagata ccacagacgc cgtgcgcgac cctcagaccc 11700
tggagatcct ggacatcaca ccatgctcct tcggcggcgt gtctgtgatc acaccaggca 11760
ccaatacaag caaccaggtg gccgtgctgt atcaggacgt gaattgtacc gaggtgccag 11820
tggcaatcca cgcagatcag ctgaccccta catggcgggt gtactctacc ggcagcaacg 11880
tgttccagac aagagccgga tgcctgatcg gagcagagca cgtgaacaat agctatgagt 11940
gcgacatccc tatcggcgcc ggcatctgtg cctcctacca gacccagaca aactccccaa 12000
ggagagcacg gtctgtggcc agccagtcca tcatcgccta taccatgagc ctgggcgccg 12060
agaattccgt ggcctactcc aacaattcta tcgccatccc taccaacttc acaatctccg 12120
tgaccacaga gatcctgcca gtgagcatga ccaagacatc cgtggactgc acaatgtata 12180
tctgtggcga ttccaccgag tgctctaacc tgctgctgca gtacggctct ttttgtaccc 12240
agctgaatag agccctgaca ggcatcgccg tggagcagga caagaacaca caggaggtgt 12300
tcgcccaggt gaagcaaatc tacaagaccc cacccatcaa ggactttggc ggcttcaact 12360
tcagccagat cctgcccgat cctagcaagc catccaagcg gtcttttatc gaggacctgc 12420
tgttcaacaa ggtgaccctg gccgatgccg gcttcatcaa gcagtatggc gattgcctgg 12480
gcgacatcgc cgccagagac ctgatctgtg cccagaagtt taatggcctg accgtgctgc 12540
ctccactgct gacagatgag atgatcgccc agtacacatc tgccctgctg gccggaacca 12600
tcacaagcgg atggaccttc ggcgcaggag ccgccctgca gatccccttt gccatgcaga 12660
tggcctatcg gttcaacggc atcggcgtga cccagaatgt gctgtacgag aaccagaagc 12720
tgatcgccaa tcagtttaac tccgccatcg gcaagatcca ggactctctg agctccacag 12780
ccagcgccct gggcaagctg caggatgtgg tgaatcagaa cgcccaggcc ctgaataccc 12840
tggtgaagca gctgtctagc aacttcggcg ccatctcctc tgtgctgaat gacatcctga 12900
gccggctgga caaggtggag gcagaggtgc agatcgaccg gctgatcaca ggcagactgc 12960
agtccctgca gacctacgtg acacagcagc tgatcagggc agcagagatc agggcctctg 13020
ccaatctggc cgccaccaag atgagcgagt gcgtgctggg ccagtccaag agagtggact 13080
tttgtggcaa gggctatcac ctgatgagct tcccacagtc cgcccctcac ggagtggtgt 13140
ttctgcacgt gacctacgtg ccagcccagg agaagaactt caccacagca ccagcaatct 13200
gccacgatgg caaggcacac tttcctaggg agggcgtgtt cgtgagcaac ggcacccact 13260
ggtttgtgac acagcgcaat ttctacgagc cacagatcat caccacagac aatacattcg 13320
tgtccggcaa ctgtgacgtg gtcatcggca tcgtgaacaa taccgtgtat gatcctctgc 13380
agccagagct ggactctttt aaggaggagc tggataagta cttcaagaat cacaccagcc 13440
ccgacgtgga tctgggcgac atctctggca tcaatgccag cgtggtgaac atccagaagg 13500
agatcgacag gctgaacgag gtggccaaga atctgaacga gtccctgatc gatctgcagg 13560
agctgggcaa gtatgagcag tacatcaagt ggccctggta tatctggctg ggcttcatcg 13620
ccggcctgat cgccatcgtg atggtgacca tcatgctgtg ctgtatgaca agctgctgtt 13680
cctgcctgaa gggctgctgt tcttgtggca gctgctgtaa gtttgatgag gacgatagcg 13740
agcctgtgct gaagggcgtg aagctgcact acacctgagc tagcgatcgc actagtgtga 13800
aatagacatc agaattaaga aaaacgtagg gtccaagtgg ttccccgtta tggactcgct 13860
atctgtcaac cagatcttat accctgaagt tcacctagat agcccgatag ttaccaataa 13920
gatagtagcc atcctggagt atgctcgagt ccctcacgct tacagcctgg aggaccctac 13980
actgtgtcag aacatcaagc accgcctaaa aaacggattt tccaaccaaa tgattataaa 14040
caatgtggaa gttgggaatg tcatcaagtc caagcttagg agttatccgg cccactctca 14100
tattccatat ccaaattgta atcaggattt atttaacata gaagacaaag agtcaacgag 14160
gaagatccgt gaactcctca aaaaggggaa ttcgctgtac tccaaagtca gtgataaggt 14220
tttccaatgc ttaagggaca ctaactcacg gcttggccta ggctccgaat tgagggagga 14280
catcaaggag aaagttatta acttgggagt ttacatgcac agctcccagt ggtttgagcc 14340
ctttctgttt tggtttacag tcaagactga gatgaggtca gtgattaaat cacaaaccca 14400
tacttgccat aggaggagac acacacctgt attcttcact ggtagttcag ttgagttgct 14460
aatctctcgt gaccttgttg ctataatcag taaagagtct caacatgtat attacctgac 14520
atttgaactg gttttgatgt attgtgatgt catagagggg aggttaatga cagagaccgc 14580
tatgactatt gatgctaggt atacagagct tctaggaaga gtcagataca tgtggaaact 14640
gatagatggt ttcttccctg cactcgggaa tccaacttat caaattgtag ccatgctgga 14700
gcctctttca cttgcttacc tgcagctgag ggatataaca gtagaactca gaggtgcttt 14760
ccttaaccac tgctttactg aaatacatga tgttcttgac caaaacgggt tttctgatga 14820
aggtacttat catgagttaa ctgaagctct agattacatt ttcataactg atgacataca 14880
tctgacaggg gagattttct catttttcag aagtttcggc caccccagac ttgaagcagt 14940
aacggctgct gaaaatgtta ggaaatacat gaatcagcct aaagtcattg tgtatgagac 15000
tctgatgaaa ggtcatgcca tattttgtgg aatcataatc aacggctatc gtgacaggca 15060
cggaggcagt tggccaccgc tgaccctccc cctgcatgct gcagacacaa tccggaatgc 15120
tcaagcttca ggtgaagggt taacacatga gcagtgcgtt gataactgga aatcttttgc 15180
tggagtgaaa tttggctgct ttatgcctct tagcctggat agtgatctga caatgtacct 15240
aaaggacaag gcacttgctg ctctccaaag ggaatgggat tcagtttacc cgaaagagtt 15300
cctgcgttac gaccctccca agggaaccgg gtcacggagg cttgtagatg ttttccttaa 15360
tgattcgagc tttgacccat atgatgtgat aatgtatgtt gtaagtggag cttacctcca 15420
tgaccctgag ttcaacctgt cttacagcct gaaagaaaag gagatcaagg aaacaggtag 15480
actttttgct aaaatgactt acaaaatgag ggcatgccaa gtgattgctg aaaatctaat 15540
ctcaaacggg attggcaaat attttaagga caatgggatg gccaaggatg agcacgattt 15600
gactaaggca ctccacactc tagctgtctc aggagtcccc aaagatctca aagaaagtca 15660
cagggggggg ccagtcttaa aaacctactc ccgaagccca gtccacacaa gtaccaggaa 15720
cgtgagagca gcaaaagggt ttatagggtt ccctcaagta attcggcagg accaagacac 15780
tgatcatccg gagaatatgg aagcttacga gacagtcagt gcatttatca cgactgatct 15840
caagaagtac tgccttaatt ggagatatga gaccatcagc ttgtttgcac agaggctaaa 15900
tgagatttac ggattgccct catttttcca gtggctgcat aagaggcttg agacctctgt 15960
cctgtatgta agtgaccctc attgcccccc cgaccttgac gcccatatcc cgttatataa 16020
agtccccaat gatcaaatct tcattaagta ccctatggga ggtatagaag ggtattgtca 16080
gaagctgtgg accatcagca ccattcccta tctatacctg gctgcttatg agagcggagt 16140
aaggattgct tcgttagtgc aaggggacaa tcagaccata gccgtaacaa aaagggtacc 16200
cagcacatgg ccctacaacc ttaagaaacg ggaagctgct agagtaacta gagattactt 16260
tgtaattctt aggcaaaggc tacatgatat tggccatcac ctcaaggcaa atgagacaat 16320
tgtttcatca catttttttg tctattcaaa aggaatatat tatgatgggc tacttgtgtc 16380
ccaatcactc aagagcatcg caagatgtgt attctggtca gagactatag ttgatgaaac 16440
aagggcagca tgcagtaata ttgctacaac aatggctaaa agcatcgaga gaggttatga 16500
ccgttacctt gcatattccc tgaacgtcct aaaagtgata cagcaaattc tgatctctct 16560
tggcttcaca atcaattcaa ccatgacccg ggatgtagtc atacccctcc tcacaaacaa 16620
cgacctctta ataaggatgg cactgttgcc cgctcctatt ggggggatga attatctgaa 16680
tatgagcagg ctgtttgtca gaaacatcgg tgatccagta acatcatcaa ttgctgatct 16740
caagagaatg attctcgcct cactaatgcc tgaagagacc ctccatcagg taatgacaca 16800
acaaccgggg gactcttcat tcctagactg ggctagcgac ccttactcag caaatcttgt 16860
atgtgtccag agcatcacta gactcctcaa gaacataact gcaaggtttg tcctgatcca 16920
tagtccaaac ccaatgttaa aaggattatt ccatgatgac agtaaagaag aggacgaggg 16980
actggcggca ttcctcatgg acaggcatat tatagtacct agggcagctc atgaaatcct 17040
ggatcatagt gtcacagggg caagagagtc tattgcaggc atgctggata ccacaaaagg 17100
cttgattcga gccagcatga ggaagggggg tttaacctct cgagtgataa ccagattgtc 17160
caattatgac tatgaacaat tcagagcagg gatggtgcta ttgacaggaa gaaagagaaa 17220
tgtcctcatt gacaaagagt catgttcagt gcagctggcg agagctctaa gaagccatat 17280
gtgggcgagg ctagctcgag gacggcctat ttacggcctt gaggtccctg atgtactaga 17340
atctatgcga ggccacctta ttcggcgtca tgagacatgt gtcatctgcg agtgtggatc 17400
agtcaactac ggatggtttt ttgtcccctc gggttgccaa ctggatgata ttgacaagga 17460
aacatcatcc ttgagagtcc catatattgg ttctaccact gatgagagaa cagacatgaa 17520
gcttgccttc gtaagagccc caagtcgatc cttgcgatct gctgttagaa tagcaacagt 17580
gtactcatgg gcttacggtg atgatgatag ctcttggaac gaagcctggt tgttggctag 17640
gcaaagggcc aatgtgagcc tggaggagct aagggtgatc actcccatct caacttcgac 17700
taatttagcg cataggttga gggatcgtag cactcaagtg aaatactcag gtacatccct 17760
tgtccgagtg gcgaggtata ccacaatctc caacgacaat ctctcatttg tcatatcaga 17820
taagaaggtt gatactaact ttatatacca acaaggaatg cttctagggt tgggtgtttt 17880
agaaacattg tttcgactcg agaaagatac cggatcatct aacacggtat tacatcttca 17940
cgtcgaaaca gattgttgcg tgatcccgat gatagatcat cccaggatac ccagctcccg 18000
caagctagag ctgagggcag agctatgtac caacccattg atatatgata atgcaccttt 18060
aattgacaga gatgcaacaa ggctatacac ccagagccat aggaggcacc ttgtggaatt 18120
tgttacatgg tccacacccc aactatatca cattttagct aagtccacag cactatctat 18180
gattgacctg gtaacaaaat ttgagaagga ccatatgaat gaaatttcag ctctcatagg 18240
ggatgacgat atcaatagtt tcataactga gtttctgctc atagagccaa gattattcac 18300
tatctacttg ggccagtgtg cggccatcaa ttgggcattt gatgtacatt atcatagacc 18360
atcagggaaa tatcagatgg gtgagctgtt gtcatcgttc ctttctagaa tgagcaaagg 18420
agtgtttaag gtgcttgtca atgctctaag ccacccaaag atctacaaga aattctggca 18480
ttgtggtatt atagagccta tccatggtcc ttcacttgat gctcaaaact tgcacacaac 18540
tgtgtgcaac atggtttaca catgctatat gacctacctc gacctgttgt tgaatgaaga 18600
gttagaagag ttcacatttc tcttgtgtga aagcgacgag gatgtagtac cggacagatt 18660
cgacaacatc caggcaaaac acttatgtgt tctggcagat ttgtactgtc aaccagggac 18720
ctgcccacca attcgaggtc taagaccggt agagaaatgt gcagttctaa ccgaccatat 18780
caaggcagag gctatgttat ctccagcagg atcttcgtgg aacataaatc caattattgt 18840
agaccattac tcatgctccc tgacttatct ccggcgagga tcgatcaaac agataagatt 18900
gagagttgat ccaggattca ttttcgacgc cctcgctgag gtaaatgtca gtcagccaaa 18960
gatcggcagc aacaacatct caaatatgag catcaaggct ttcagacccc cacacgatga 19020
tgttgcaaaa ttgctcaaag atatcaacac aagcaagcac aatcttccca tttcaggggg 19080
caatctcgcc aattatgaaa tccatgcttt ccgcagaatc gggttgaact catctgcttg 19140
ctacaaagct gttgagatat caacattaat taggagatgc cttgagccag gggaggacgg 19200
cttgttcttg ggtgagggat cgggttctat gttgatcact tataaggaga tacttaaact 19260
aaacaagtgc ttctataata gtggggtttc cgccaattct agatctggtc aaagggaatt 19320
agcaccctat ccctccgaag ttggccttgt cgaacacaga atgggagtag gtaatattgt 19380
caaagtgctc tttaacggga ggcccgaagt cacgtgggta ggcagtgtag attgcttcaa 19440
tttcatagtt agtaatatcc ctacctctag tgtggggttt atccattcag atatagagac 19500
cttgcctgac aaagatacta tagagaagct agaggaattg gcagccatct tatcgatggc 19560
tctgctcctg ggcaaaatag gatcaatact ggtgattaag cttatgcctt tcagcgggga 19620
ttttgttcag ggatttataa gttatgtagg gtctcattat agagaagtga accttgtata 19680
ccctagatac agcaacttca tatctactga atcttatttg gttatgacag atctcaaggc 19740
taaccggcta atgaatcctg aaaagattaa gcagcagata attgaatcat ctgtgaggac 19800
ttcacctgga cttataggtc acatcctatc cattaagcaa ctaagctgca tacaagcaat 19860
tgtgggagac gcagttagta gaggtgatat caatcctact ctgaaaaaac ttacacctat 19920
agagcaggtg ctgatcaatt gcgggttggc aattaacgga cctaagctgt gcaaagaatt 19980
gatccaccat gatgttgcct cagggcaaga tggattgctt aattctatac tcatcctcta 20040
cagggagttg gcaagattca aagacaacca aagaagtcaa caagggatgt tccacgccta 20100
ccccgtattg gtaagtagca ggcaacgaga acttatatct aggatcaccc gcaaattttg 20160
ggggcacatt cttctttact ccgggaacaa aaagttgata aataagttta tccagaatct 20220
caagtccggc tatctgatac tagacttaca ccagaatatc ttcgttaaga atctatccaa 20280
gtcagagaaa cagattatta tgacgggggg tttgaaacgt gagtgggttt ttaaggtaac 20340
agtcaaggag accaaagaat ggtataagtt agtcggatac agtgccctga ttaaggacta 20400
attggttgaa ctccggaacc ctaatcctgc cctaggtggt taggcattat ttgcaatata 20460
ttaaagaaaa ctttgaaaat acgaagtttc tattcccagc tttgtctggt ggccggcata 20520
gtcccagcct cctcgctggc gctggctggg caacattccg aggggaccgt ccccacggta 20580
atggcgaatg ggacgcggcc gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg 20640
ctgctggcgc tggctgggca ataactagca taaccccttg gggcctctaa acgggtcttg 20700
aggggttttt tgctgaaagg aggaactata tccggatgcg gccgcgcgct tggcgtaatc 20760
atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 20820
agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 20880
tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 20940
aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 21000
cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 21060
ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 21120
ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 21180
cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 21240
actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 21300
cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 21360
tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 21420
gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 21480
caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 21540
agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 21600
tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 21660
tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 21720
gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 21780
gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 21840
aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 21900
atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 21960
gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 22020
acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 22080
ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 22140
tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 22200
ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 22260
ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 22320
atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 22380
taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 22440
catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 22500
atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 22560
acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 22620
aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 22680
ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 22740
cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 22800
atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 22860
ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctg 22916
<210> 6
<211> 13907
<212> DNA
<213> 人工序列
<220>
<223> RABV载体: Coravax V1-China (RABVG-E31)
<400> 6
acgcttaaca accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa 60
cacccctaca atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt 120
gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa 180
aaagccctgt ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt 240
gtcaggcatg agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc 300
aatgcagttt tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc 360
acgaaaagga gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga 420
agggaattgg gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca 480
tgcgtcctta gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa 540
cactggtaac tataagacaa acattgcaga caggatagag cagatttttg agacagcccc 600
ttttgttaaa atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg 660
gagtactata ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat 720
tgagcatcta tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc 780
aggactggta tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat 840
actatatttc ttccacaaga actttgagga agagataaga agaatgtttg agccagggca 900
ggagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa 960
atctccttat tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg 1020
ctatatgggt caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga 1080
aatgtctgtt ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag 1140
aagattcttc agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac 1200
tgacgtagca ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg 1260
tgaaaccaga agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa 1320
gagatctcac atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc 1380
attcgccgag tttctaaaca agacatattc gagtgactca taacatgaaa aaaactaaca 1440
cccctcccgt acgccaccat gttcgtgttt ctggtgctgc tgcctctggt gagctcccag 1500
tgcgtgaacc tgaccacaag gacccagctg ccccctgcct ataccaattc cttcacacgg 1560
ggcgtgtact atcccgacaa ggtgttccgg agcagcgtgc tgcactccac acaggatctg 1620
tttctgcctt tcttttctaa cgtgacctgg ttccacgcca tccacgtgag cggcaccaat 1680
ggcacaaagc ggttcgacaa tccagtgctg ccctttaacg atggcgtgta cttcgcctcc 1740
accgagaagt ctaacatcat cagaggctgg atctttggca ccacactgga cagcaagaca 1800
cagtccctgc tgatcgtgaa caatgccacc aacgtggtca tcaaggtgtg cgagttccag 1860
ttttgtaatg atccattcct gggcgtgtac tatcacaaga acaataagtc ttggatggag 1920
agcgagtttc gcgtgtattc ctctgccaac aattgcacat ttgagtacgt gtcccagccc 1980
ttcctgatgg acctggaggg caagcagggc aatttcaaga acctgaggga gttcgtgttt 2040
aagaatatcg atggctactt caaaatctac tccaagcaca ccccaatcaa cctggtgcgc 2100
gacctgccac agggcttctc tgccctggag ccactggtgg atctgcccat cggcatcaac 2160
atcacccggt ttcagacact gctggccctg cacagaagct acctgacacc aggcgacagc 2220
tcctctggat ggaccgcagg agcagcagcc tactatgtgg gctatctgca gcccaggacc 2280
ttcctgctga agtacaacga gaatggcacc atcacagacg ccgtggattg cgccctggat 2340
cccctgtctg agaccaagtg tacactgaag agctttaccg tggagaaggg catctatcag 2400
acaagcaatt tcagggtgca gcctaccgag tccatcgtgc gctttcccaa tatcacaaac 2460
ctgtgccctt ttggcgaggt gttcaacgca acccgcttcg ccagcgtgta cgcctggaat 2520
aggaagcgca tctccaactg cgtggccgac tattctgtgc tgtacaacag cgcctccttc 2580
tctaccttta agtgctatgg cgtgagcccc acaaagctga atgacctgtg ctttaccaac 2640
gtgtacgccg attccttcgt gatcaggggc gacgaggtgc gccagatcgc accaggacag 2700
acaggcaaga tcgcagacta caattataag ctgcctgacg atttcaccgg ctgcgtgatc 2760
gcctggaact ctaacaatct ggatagcaaa gtgggcggca actacaatta tctgtaccgg 2820
ctgtttagaa agtctaatct gaagccattc gagagggaca tctccacaga aatctaccag 2880
gccggctcta ccccctgcaa tggcgtggag ggctttaact gttatttccc tctgcagagc 2940
tacggcttcc agccaacaaa cggcgtgggc tatcagccct accgcgtggt ggtgctgtct 3000
tttgagctgc tgcacgcacc tgcaacagtg tgcggaccaa agaagagcac caatctggtg 3060
aagaacaagt gcgtgaactt caacttcaac ggactgaccg gaacaggcgt gctgaccgag 3120
tccaacaaga agttcctgcc ttttcagcag ttcggcaggg acatcgcaga taccacagac 3180
gccgtgcgcg accctcagac cctggagatc ctggacatca caccatgctc cttcggcggc 3240
gtgtctgtga tcacaccagg caccaataca agcaaccagg tggccgtgct gtatcaggac 3300
gtgaattgta ccgaggtgcc agtggcaatc cacgcagatc agctgacccc tacatggcgg 3360
gtgtactcta ccggcagcaa cgtgttccag acaagagccg gatgcctgat cggagcagag 3420
cacgtgaaca atagctatga gtgcgacatc cctatcggcg ccggcatctg tgcctcctac 3480
cagacccaga caaactcccc aaggtctgtg ggagatgagg ccgaagactt tgtggaagtc 3540
cacctgcctg atgtgcataa ccaggtgtct ggcgtcgacc tgggactgcc aaattggggc 3600
aagtacgtgc tgctgagtgc tggagcactg actgccctga tgctgatcat tttcctgatg 3660
acctgctgtc ggcgcgtgaa cagaagtgag cccactcagc acaatctgcg aggaaccggg 3720
agagaagtgt cagtcacacc tcagagcggg aaaatcatta gtagttggga atcacataaa 3780
agcgggggcg agaccaggct gtgagctagc catgaaaaaa actaacaccc ctcctttcga 3840
accatcccaa acatgagcaa gatctttgtc aatcctagtg ctattagagc cggtctggcc 3900
gatcttgaga tggctgaaga aactgttgat ctgatcaata gaaatatcga agacaatcag 3960
gctcatctcc aaggggaacc catagaggtg gacaatctcc ctgaggatat ggggcgactt 4020
cacctggatg atggaaaatc gcccaaccat ggtgagatag ccaaggtggg agaaggcaag 4080
tatcgagagg actttcagat ggatgaagga gaggatccta gcttcctgtt ccagtcatac 4140
ctggaaaatg ttggagtcca aatagtcaga caaatgaggt caggagagag atttctcaag 4200
atatggtcac agaccgtaga agagattata tcctatgtcg cggtcaactt tcccaaccct 4260
ccaggaaagt cttcagagga taaatcaacc cagactactg gccgagagct caagaaggag 4320
acaacaccca ctccttctca gagagaaagc caatcatcga aagccaggat ggcggctcaa 4380
attgcttctg gccctccagc ccttgaatgg tcggctacca atgaagagga tgatctatca 4440
gtggaggctg agatcgctca ccagattgca gaaagtttct ccaaaaaata taagtttccc 4500
tctcgatcct cagggatact cttgtataat tttgagcaat tgaaaatgaa ccttgatgat 4560
atagttaaag aggcaaaaaa tgtaccaggt gtgacccgtt tagcccatga cgggtccaaa 4620
ctccccctaa gatgtgtact gggatgggtc gctttggcca actctaagaa attccagttg 4680
ttagtcgaat ccgacaagct gagtaaaatc atgcaagatg acttgaatcg ctatacatct 4740
tgctaaccga acctctcccc tcagtccctc tagacaataa aatccgagat gtcccaaagt 4800
caacatgaaa aaaacaggca acaccactga taaaatgaac ctcctacgta agatagtgaa 4860
aaaccgcagg gacgaggaca ctcaaaaatc ctctcccgcg tcagcccctc tggatgacga 4920
tgacttgtgg cttccacccc ctgaatacgt cccgctgaaa gaacttacag gcaagaagaa 4980
catgaggaac ttttgtatca acggaagggt taaagtgtgt agcccgaatg gttactcgtt 5040
caggatcctg cggcacattc tgaaatcatt cgacgagata tattctggga atcataggat 5100
gatcgggtta gtcaaagtgg ttattggact ggctttgtca ggatctccag tccctgaggg 5160
cctgaactgg gtatacaaat tgaggagaac ctttatcttc cagtgggctg attccagggg 5220
ccctcttgaa ggggaggagt tggaatactc tcaggagatc acttgggatg atgatactga 5280
gttcgtcgga ttgcaaataa gagtgattgc aaaacagtgt catatccagg gcagagtctg 5340
gtgtatcaac atgaacccga gagcatgtca actatggtct gacatgtctc ttcagacaca 5400
aaggtccgaa gaggacaaag attcctctct gcttctagaa taatcagatt atatcccgca 5460
aatttatcac ttgtttacct ctggaggaga gaacatatgg gctcaactcc aacccttggg 5520
agcaatataa caaaaaacat gttatggtgc cattaaaccg ctgcatttca tcaaagtcaa 5580
gttgattacc tttacatttt gatcctcttg gatgtgaaaa aaactattaa catccctcaa 5640
aagaccccgg gaaagatggt tcctcaggct ctcctgtttg taccccttct ggtttttcca 5700
ttgtgttttg ggaaattccc tatttacacg ataccagaca agcttggtcc ctggagtccg 5760
attgacatac atcacctcag ctgcccaaac aatttggtag tggaggacga aggatgcacc 5820
aacctgtcag ggttctccta catggaactt aaagttggat acatcttagc cataaaagtg 5880
aacgggttca cttgcacagg cgttgtgacg gaggctgaaa cctacactaa cttcgttggt 5940
tatgtcacaa ccacgttcaa aagaaagcat ttccgcccaa caccagatgc atgtagagcc 6000
gcgtacaact ggaagatggc cggtgacccc agatatgaag agtctctaca caatccgtac 6060
cctgactacc gctggcttcg aactgtaaaa accaccaagg agtctctcgt tatcatatct 6120
ccaagtgtgg cagatttgga cccatatgac agatcccttc actcgagggt cttccctagc 6180
gggaagtgct caggagtagc ggtgtcttct acctactgct ccactaacca cgattacacc 6240
atttggatgc ccgagaatcc gagactaggg atgtcttgtg acatttttac caatagtaga 6300
gggaagagag catccaaagg gagtgagact tgcggctttg tagatgaaag aggcctatat 6360
aagtctttaa aaggagcatg caaactcaag ttatgtggag ttctaggact tagacttatg 6420
gatggaacat gggtctcgat gcaaacatca aatgaaacca aatggtgccc tcccgataag 6480
ttggtgaacc tgcacgactt tcgctcagac gaaattgagc accttgttgt agaggagttg 6540
gtcaggaaga gagaggagtg tctggatgca ctagagtcca tcatgacaac caagtcagtg 6600
agtttcagac gtctcagtca tttaagaaaa cttgtccctg ggtttggaaa agcatatacc 6660
atattcaaca agaccttgat ggaagccgat gctcactaca agtcagtcga gacttggaat 6720
gagatcctcc cttcaaaagg gtgtttaaga gttgggggga ggtgtcatcc tcatgtgaac 6780
ggggtgtttt tcaatggtat aatattagga cctgacggca atgtcttaat cccagagatg 6840
caatcatccc tcctccagca acatatggag ttgttggaat cctcggttat cccccttgtg 6900
caccccctgg cagacccgtc taccgttttc aaggacggtg acgaggctga ggattttgtt 6960
gaagttcacc ttcccgatgt gcacaatcag gtctcaggag ttgacttggg tctcccgaac 7020
tgggggaagt atgtattact gagtgcaggg gccctgactg ccttgatgtt gataattttc 7080
ctgatgacat gttgtagaag agtcaatcga tcagaaccta cgcaacacaa tctcagaggg 7140
acagggaggg aggtgtcagt cactccccaa agcgggaaga tcatatcttc atgggaatca 7200
cacaagagtg ggggtgagac cagactgtaa ttaattaacg tcctttcaac gatccaagtc 7260
catgaaaaaa actaacaccc ctcccgtacc tagcttataa agtgctgggt catctaagct 7320
tttcagtcga gaaaaaaaca ttagatcaga agaacaactg gcaacacttc tcaacctgag 7380
acttacttca agatgctcga tcctggagag gtctatgatg accctattga cccaatcgag 7440
ttagaggctg aacccagagg aacccccatt gtccccaaca tcttgaggaa ctctgactac 7500
aatctcaact ctcctttgat agaagatcct gctagactaa tgttagaatg gttaaaaaca 7560
gggaatagac cttatcggat gactctaaca gacaattgct ccaggtcttt cagagttttg 7620
aaagattatt tcaagaaggt agatttgggt tctctcaagg tgggcggaat ggctgcacag 7680
tcaatgattt ctctctggtt atatggtgcc cactctgaat ccaacaggag ccggagatgt 7740
ataacagact tggcccattt ctattccaag tcgtccccca tagagaagct gttgaatctc 7800
acgctaggaa atagagggct gagaatcccc ccagagggag tgttaagttg ccttgagagg 7860
gttgattatg ataatgcatt tggaaggtat cttgccaaca cgtattcctc ttacttgttc 7920
ttccatgtaa tcaccttata catgaacgcc ctagactggg atgaagaaaa gaccatccta 7980
gcattatgga aagatttaac ctcagtggac atcgggaagg acttggtaaa gttcaaagac 8040
caaatatggg gactgctgat cgtgacaaag gactttgttt actcccaaag ttccaattgt 8100
ctttttgaca gaaactacac acttatgcta aaagatcttt tcttgtctcg cttcaactcc 8160
ttaatggtct tgctctctcc cccagagccc cgatactcag atgacttgat atctcaacta 8220
tgccagctgt acattgctgg ggatcaagtc ttgtctatgt gtggaaactc cggctatgaa 8280
gtcatcaaaa tattggagcc atatgtcgtg aatagtttag tccagagagc agaaaagttt 8340
aggcctctca ttcattcctt gggagacttt cctgtattta taaaagacaa ggtaagtcaa 8400
cttgaagaga cgttcggtcc ctgtgcaaga aggttcttta gggctctgga tcaattcgac 8460
aacatacatg acttggtttt tgtgtttggc tgttacaggc attgggggca cccatatata 8520
gattatcgaa agggtctgtc aaaactatat gatcaggttc accttaaaaa aatgatagat 8580
aagtcctacc aggagtgctt agcaagcgac ctagccagga ggatccttag atggggtttt 8640
gataagtact ccaagtggta tctggattca agattcctag cccgagacca ccccttgact 8700
ccttatatca aaacccaaac atggccaccc aaacatattg tagacttggt gggggataca 8760
tggcacaagc tcccgatcac gcagatcttt gagattcctg aatcaatgga tccgtcagaa 8820
atattggatg acaaatcaca ttctttcacc agaacgagac tagcttcttg gctgtcagaa 8880
aaccgagggg ggcctgttcc tagcgaaaaa gttattatca cggccctgtc taagccgcct 8940
gtcaatcccc gagagtttct gaggtctata gacctcggag gattgccaga tgaagacttg 9000
ataattggcc tcaagccaaa ggaacgggaa ttgaagattg aaggtcgatt ctttgctcta 9060
atgtcatgga atctaagatt gtattttgtc atcactgaaa aactcttggc caactacatc 9120
ttgccacttt ttgacgcgct gactatgaca gacaacctga acaaggtgtt taaaaagctg 9180
atcgacaggg tcaccgggca agggcttttg gactattcaa gggtcacata tgcatttcac 9240
ctggactatg aaaagtggaa caaccatcaa agattagagt caacagagga tgtattttct 9300
gtcctagatc aagtgtttgg attgaagaga gtgttttcta gaacacacga gttttttcaa 9360
aaggcctgga tctattattc agacagatca gacctcatcg ggttacggga ggatcaaata 9420
tactgcttag atgcgtccaa cggcccaacc tgttggaatg gccaggatgg cgggctagaa 9480
ggcttacggc agaagggctg gagtctagtc agcttattga tgatagatag agaatctcaa 9540
atcaggaaca caagaaccaa aatactagct caaggagaca accaggtttt atgtccgaca 9600
tacatgttgt cgccagggct atctcaagag gggctcctct atgaattgga gagaatatca 9660
aggaatgcac tttcgatata cagagccgtc gaggaagggg catctaagct agggctgatc 9720
atcaagaaag aagagaccat gtgtagttat gacttcctca tctatggaaa aacccctttg 9780
tttagaggta acatattggt gcctgagtcc aaaagatggg ccagagtctc ttgcgtctct 9840
aatgaccaaa tagtcaacct cgccaatata atgtcgacag tgtccaccaa tgcgctaaca 9900
gtggcacaac actctcaatc tttgatcaaa ccgatgaggg attttctgct catgtcagta 9960
caggcagtct ttcactacct gctatttagc ccaatcttaa agggaagagt ttacaagatt 10020
ctgagcgctg aaggggagag ctttctccta gccatgtcaa ggataatcta tctagatcct 10080
tctttgggag ggatatctgg aatgtccctc ggaagattcc atatacgaca gttctcagac 10140
cctgtctctg aagggttatc cttctggaga gagatctggt taagctccca agagtcctgg 10200
attcacgcgt tgtgtcaaga ggctggaaac ccagatcttg gagagagaac actcgagagc 10260
ttcactcgcc ttctagaaga tccgaccacc ttaaatatca gaggaggggc cagtcctacc 10320
attctactca aggatgcaat cagaaaggct ttatatgacg aggtggacaa ggtggaaaat 10380
tcagagtttc gagaggcaat cctgttgtcc aagacccata gagataattt tatactcttc 10440
ttaatatctg ttgagcctct gtttcctcga tttctcagtg agctattcag ttcgtctttt 10500
ttgggaatcc ccgagtcaat cattggattg atacaaaact cccgaacgat aagaaggcag 10560
tttagaaaga gtctctcaaa aactttagaa gaatccttct acaactcaga gatccacggg 10620
attagtcgga tgacccagac acctcagagg gttggggggg tgtggccttg ctcttcagag 10680
agggcagatc tacttaggga gatctcttgg ggaagaaaag tggtaggcac gacagttcct 10740
cacccttctg agatgttggg attacttccc aagtcctcta tttcttgcac ttgtggagca 10800
acaggaggag gcaatcctag agtttctgta tcagtactcc cgtcctttga tcagtcattt 10860
ttttcacgag gccccctaaa gggatacttg ggctcgtcca cctctatgtc gacccagcta 10920
ttccatgcat gggaaaaagt cactaatgtt catgtggtga agagagctct atcgttaaaa 10980
gaatctataa actggttcat tactagagat tccaacttgg ctcaagctct aattaggaac 11040
attatgtctc tgacaggccc tgatttccct ctagaggagg cccctgtctt caaaaggacg 11100
gggtcagcct tgcataggtt caagtctgcc agatacagcg aaggagggta ttcttctgtc 11160
tgcccgaacc tcctctctca tatttctgtt agtacagaca ccatgtctga tttgacccaa 11220
gacgggaaga actacgattt catgttccag ccattgatgc tttatgcaca gacatggaca 11280
tcagagctgg tacagagaga cacaaggcta agagactcta cgtttcattg gcacctccga 11340
tgcaacaggt gtgtgagacc cattgacgac gtgaccctgg agacctctca gatcttcgag 11400
tttccggatg tgtcgaaaag aatatccaga atggtttctg gggctgtgcc tcacttccag 11460
aggcttcccg atatccgtct gagaccagga gattttgaat ctctaagcgg tagagaaaag 11520
tctcaccata tcggatcagc tcaggggctc ttatactcaa tcttagtggc aattcacgac 11580
tcaggataca atgatggaac catcttccct gtcaacatat acggcaaggt ttcccctaga 11640
gactatttga gagggctcgc aaggggagta ttgataggat cctcgatttg cttcttgaca 11700
agaatgacaa atatcaatat taatagacct cttgaattgg tctcaggggt aatctcatat 11760
attctcctga ggctagataa ccatccctcc ttgtacataa tgctcagaga accgtctctt 11820
agaggagaga tattttctat ccctcagaaa atccccgccg cttatccaac cactatgaaa 11880
gaaggcaaca gatcaatctt gtgttatctc caacatgtgc tacgctatga gcgagagata 11940
atcacggcgt ctccagagaa tgactggcta tggatctttt cagactttag aagtgccaaa 12000
atgacgtacc tatccctcat tacttaccag tctcatcttc tactccagag ggttgagaga 12060
aacctatcta agagtatgag agataacctg cgacaattga gttctttgat gaggcaggtg 12120
ctgggcgggc acggagaaga taccttagag tcagacgaca acattcaacg actgctaaaa 12180
gactctttac gaaggacaag atgggtggat caagaggtgc gccatgcagc tagaaccatg 12240
actggagatt acagccccaa caagaaggtg tcccgtaagg taggatgttc agaatgggtc 12300
tgctctgctc aacaggttgc agtctctacc tcagcaaacc cggcccctgt ctcggagctt 12360
gacataaggg ccctctctaa gaggttccag aaccctttga tctcgggctt gagagtggtt 12420
cagtgggcaa ccggtgctca ttataagctt aagcctattc tagatgatct caatgttttc 12480
ccatctctct gccttgtagt tggggacggg tcagggggga tatcaagggc agtcctcaac 12540
atgtttccag atgccaagct tgtgttcaac agtcttttag aggtgaatga cctgatggct 12600
tccggaacac atccactgcc tccttcagca atcatgaggg gaggaaatga tatcgtctcc 12660
agagtgatag atcttgactc aatctgggaa aaaccgtccg acttgagaaa cttggcaacc 12720
tggaaatact tccagtcagt ccaaaagcag gtcaacatgt cctatgacct cattatttgc 12780
gatgcagaag ttactgacat tgcatctatc aaccggatca ccctgttaat gtccgatttt 12840
gcattgtcta tagatggacc actctatttg gtcttcaaaa cttatgggac tatgctagta 12900
aatccaaact acaaggctat tcaacacctg tcaagagcgt tcccctcggt cacagggttt 12960
atcacccaag taacttcgtc tttttcatct gagctctacc tccgattctc caaacgaggg 13020
aagtttttca gagatgctga gtacttgacc tcttccaccc ttcgagaaat gagccttgtg 13080
ttattcaatt gtagcagccc caagagtgag atgcagagag ctcgttcctt gaactatcag 13140
gatcttgtga gaggatttcc tgaagaaatc atatcaaatc cttacaatga gatgatcata 13200
actctgattg acagtgatgt agaatctttt ctagtccaca agatggttga tgatcttgag 13260
ttacagaggg gaactctgtc taaagtggct atcattatag ccatcatgat agttttctcc 13320
aacagagtct tcaacgtttc caaaccccta actgacccct cgttctatcc accgtctgat 13380
cccaaaatcc tgaggcactt caacatatgt tgcagtacta tgatgtatct atctactgct 13440
ttaggtgacg tccctagctt cgcaagactt cacgacctgt ataacagacc tataacttat 13500
tacttcagaa agcaagtcat tcgagggaac gtttatctat cttggagttg gtccaacgac 13560
acctcagtgt tcaaaagggt agcctgtaat tctagcctga gtctgtcatc tcactggatc 13620
aggttgattt acaagatagt gaagactacc agactcgttg gcagcatcaa ggatctatcc 13680
agagaagtgg aaagacacct tcataggtac aacaggtgga tcaccctaga ggatatcaga 13740
tctagatcat ccctactaga ctacagttgc ctgtgaaccg gatactcctg gaagcctgcc 13800
catgctaaga ctcttgtgtg atgtatcttg aaaaaaacaa gatcctaaat ctgaaccttt 13860
ggttgtttga ttgtttttct catttttgtt gtttatttgt taagcgt 13907
<210> 7
<211> 13907
<212> DNA
<213> 人工序列
<220>
<223> RABV载体: Coravax V1-South Africa (RABVG-E31)
<400> 7
acgcttaaca accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa 60
cacccctaca atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt 120
gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa 180
aaagccctgt ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt 240
gtcaggcatg agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc 300
aatgcagttt tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc 360
acgaaaagga gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga 420
agggaattgg gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca 480
tgcgtcctta gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa 540
cactggtaac tataagacaa acattgcaga caggatagag cagatttttg agacagcccc 600
ttttgttaaa atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg 660
gagtactata ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat 720
tgagcatcta tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc 780
aggactggta tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat 840
actatatttc ttccacaaga actttgagga agagataaga agaatgtttg agccagggca 900
ggagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa 960
atctccttat tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg 1020
ctatatgggt caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga 1080
aatgtctgtt ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag 1140
aagattcttc agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac 1200
tgacgtagca ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg 1260
tgaaaccaga agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa 1320
gagatctcac atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc 1380
attcgccgag tttctaaaca agacatattc gagtgactca taacatgaaa aaaactaaca 1440
cccctcccgt acgccaccat gttcgtgttt ctggtgctgc tgcctctggt gagctcccag 1500
tgcgtgaact tcaccacaag gacccagctg ccccctgcct ataccaattc cttcacacgg 1560
ggcgtgtact atcccgacaa ggtgttccgg agcagcgtgc tgcactccac acaggatctg 1620
tttctgcctt tcttttctaa cgtgacctgg ttccacgcca tccacgtgag cggcaccaat 1680
ggcacaaagc ggttcgccaa tccagtgctg ccctttaacg atggcgtgta cttcgcctcc 1740
accgagaagt ctaacatcat cagaggctgg atctttggca ccacactgga cagcaagaca 1800
cagtccctgc tgatcgtgaa caatgccacc aacgtggtca tcaaggtgtg cgagttccag 1860
ttttgtaatg atccattcct gggcgtgtac tatcacaaga acaataagtc ttggatggag 1920
agcgagtttc gcgtgtattc ctctgccaac aattgcacat ttgagtacgt gtcccagccc 1980
ttcctgatgg acctggaggg caagcagggc aatttcaaga acctgaggga gttcgtgttt 2040
aagaatatcg atggctactt caaaatctac tccaagcaca ccccaatcaa cctggtgcgc 2100
ggcctgccac agggcttctc tgccctggag ccactggtgg atctgcccat cggcatcaac 2160
atcacccggt ttcagacact gctggccctg cacagaagct acctgacacc aggcgacagc 2220
tcctctggat ggaccgcagg agcagcagcc tactatgtgg gctatctgca gcccaggacc 2280
ttcctgctga agtacaacga gaatggcacc atcacagacg ccgtggattg cgccctggat 2340
cccctgtctg agaccaagtg tacactgaag agctttaccg tggagaaggg catctatcag 2400
acaagcaatt tcagggtgca gcctaccgag tccatcgtgc gctttcccaa tatcacaaac 2460
ctgtgccctt ttggcgaggt gttcaacgca acccgcttcg ccagcgtgta cgcctggaat 2520
aggaagcgca tctccaactg cgtggccgac tattctgtgc tgtacaacag cgcctccttc 2580
tctaccttta agtgctatgg cgtgagcccc acaaagctga atgacctgtg ctttaccaac 2640
gtgtacgccg attccttcgt gatcaggggc gacgaggtgc gccagatcgc accaggacag 2700
acaggcaata tcgcagacta caattataag ctgcctgacg atttcaccgg ctgcgtgatc 2760
gcctggaact ctaacaatct ggatagcaaa gtgggcggca actacaatta tctgtaccgg 2820
ctgtttagaa agtctaatct gaagccattc gagagggaca tctccacaga aatctaccag 2880
gccggctcta ccccctgcaa tggcgtgaag ggctttaact gttatttccc tctgcagagc 2940
tacggcttcc agccaacata tggcgtgggc tatcagccct accgcgtggt ggtgctgtct 3000
tttgagctgc tgcacgcacc tgcaacagtg tgcggaccaa agaagagcac caatctggtg 3060
aagaacaagt gcgtgaactt caacttcaac ggactgaccg gaacaggcgt gctgaccgag 3120
tccaacaaga agttcctgcc ttttcagcag ttcggcaggg acatcgcaga taccacagac 3180
gccgtgcgcg accctcagac cctggagatc ctggacatca caccatgctc cttcggcggc 3240
gtgtctgtga tcacaccagg caccaataca agcaaccagg tggccgtgct gtatcagggc 3300
gtgaattgta ccgaggtgcc agtggcaatc cacgcagatc agctgacccc tacatggcgg 3360
gtgtactcta ccggcagcaa cgtgttccag acaagagccg gatgcctgat cggagcagag 3420
cacgtgaaca atagctatga gtgcgacatc cctatcggcg ccggcatctg tgcctcctac 3480
cagacccaga caaactcccc aaggtctgtg ggagatgagg ccgaagactt tgtggaagtc 3540
cacctgcctg atgtgcataa ccaggtgtct ggcgtcgacc tgggactgcc aaattggggc 3600
aagtacgtgc tgctgagtgc tggagcactg actgccctga tgctgatcat tttcctgatg 3660
acctgctgtc ggcgcgtgaa cagaagtgag cccactcagc acaatctgcg aggaaccggg 3720
agagaagtgt cagtcacacc tcagagcggg aaaatcatta gtagttggga atcacataaa 3780
agcgggggcg agaccaggct gtgagctagc catgaaaaaa actaacaccc ctcctttcga 3840
accatcccaa acatgagcaa gatctttgtc aatcctagtg ctattagagc cggtctggcc 3900
gatcttgaga tggctgaaga aactgttgat ctgatcaata gaaatatcga agacaatcag 3960
gctcatctcc aaggggaacc catagaggtg gacaatctcc ctgaggatat ggggcgactt 4020
cacctggatg atggaaaatc gcccaaccat ggtgagatag ccaaggtggg agaaggcaag 4080
tatcgagagg actttcagat ggatgaagga gaggatccta gcttcctgtt ccagtcatac 4140
ctggaaaatg ttggagtcca aatagtcaga caaatgaggt caggagagag atttctcaag 4200
atatggtcac agaccgtaga agagattata tcctatgtcg cggtcaactt tcccaaccct 4260
ccaggaaagt cttcagagga taaatcaacc cagactactg gccgagagct caagaaggag 4320
acaacaccca ctccttctca gagagaaagc caatcatcga aagccaggat ggcggctcaa 4380
attgcttctg gccctccagc ccttgaatgg tcggctacca atgaagagga tgatctatca 4440
gtggaggctg agatcgctca ccagattgca gaaagtttct ccaaaaaata taagtttccc 4500
tctcgatcct cagggatact cttgtataat tttgagcaat tgaaaatgaa ccttgatgat 4560
atagttaaag aggcaaaaaa tgtaccaggt gtgacccgtt tagcccatga cgggtccaaa 4620
ctccccctaa gatgtgtact gggatgggtc gctttggcca actctaagaa attccagttg 4680
ttagtcgaat ccgacaagct gagtaaaatc atgcaagatg acttgaatcg ctatacatct 4740
tgctaaccga acctctcccc tcagtccctc tagacaataa aatccgagat gtcccaaagt 4800
caacatgaaa aaaacaggca acaccactga taaaatgaac ctcctacgta agatagtgaa 4860
aaaccgcagg gacgaggaca ctcaaaaatc ctctcccgcg tcagcccctc tggatgacga 4920
tgacttgtgg cttccacccc ctgaatacgt cccgctgaaa gaacttacag gcaagaagaa 4980
catgaggaac ttttgtatca acggaagggt taaagtgtgt agcccgaatg gttactcgtt 5040
caggatcctg cggcacattc tgaaatcatt cgacgagata tattctggga atcataggat 5100
gatcgggtta gtcaaagtgg ttattggact ggctttgtca ggatctccag tccctgaggg 5160
cctgaactgg gtatacaaat tgaggagaac ctttatcttc cagtgggctg attccagggg 5220
ccctcttgaa ggggaggagt tggaatactc tcaggagatc acttgggatg atgatactga 5280
gttcgtcgga ttgcaaataa gagtgattgc aaaacagtgt catatccagg gcagagtctg 5340
gtgtatcaac atgaacccga gagcatgtca actatggtct gacatgtctc ttcagacaca 5400
aaggtccgaa gaggacaaag attcctctct gcttctagaa taatcagatt atatcccgca 5460
aatttatcac ttgtttacct ctggaggaga gaacatatgg gctcaactcc aacccttggg 5520
agcaatataa caaaaaacat gttatggtgc cattaaaccg ctgcatttca tcaaagtcaa 5580
gttgattacc tttacatttt gatcctcttg gatgtgaaaa aaactattaa catccctcaa 5640
aagaccccgg gaaagatggt tcctcaggct ctcctgtttg taccccttct ggtttttcca 5700
ttgtgttttg ggaaattccc tatttacacg ataccagaca agcttggtcc ctggagtccg 5760
attgacatac atcacctcag ctgcccaaac aatttggtag tggaggacga aggatgcacc 5820
aacctgtcag ggttctccta catggaactt aaagttggat acatcttagc cataaaagtg 5880
aacgggttca cttgcacagg cgttgtgacg gaggctgaaa cctacactaa cttcgttggt 5940
tatgtcacaa ccacgttcaa aagaaagcat ttccgcccaa caccagatgc atgtagagcc 6000
gcgtacaact ggaagatggc cggtgacccc agatatgaag agtctctaca caatccgtac 6060
cctgactacc gctggcttcg aactgtaaaa accaccaagg agtctctcgt tatcatatct 6120
ccaagtgtgg cagatttgga cccatatgac agatcccttc actcgagggt cttccctagc 6180
gggaagtgct caggagtagc ggtgtcttct acctactgct ccactaacca cgattacacc 6240
atttggatgc ccgagaatcc gagactaggg atgtcttgtg acatttttac caatagtaga 6300
gggaagagag catccaaagg gagtgagact tgcggctttg tagatgaaag aggcctatat 6360
aagtctttaa aaggagcatg caaactcaag ttatgtggag ttctaggact tagacttatg 6420
gatggaacat gggtctcgat gcaaacatca aatgaaacca aatggtgccc tcccgataag 6480
ttggtgaacc tgcacgactt tcgctcagac gaaattgagc accttgttgt agaggagttg 6540
gtcaggaaga gagaggagtg tctggatgca ctagagtcca tcatgacaac caagtcagtg 6600
agtttcagac gtctcagtca tttaagaaaa cttgtccctg ggtttggaaa agcatatacc 6660
atattcaaca agaccttgat ggaagccgat gctcactaca agtcagtcga gacttggaat 6720
gagatcctcc cttcaaaagg gtgtttaaga gttgggggga ggtgtcatcc tcatgtgaac 6780
ggggtgtttt tcaatggtat aatattagga cctgacggca atgtcttaat cccagagatg 6840
caatcatccc tcctccagca acatatggag ttgttggaat cctcggttat cccccttgtg 6900
caccccctgg cagacccgtc taccgttttc aaggacggtg acgaggctga ggattttgtt 6960
gaagttcacc ttcccgatgt gcacaatcag gtctcaggag ttgacttggg tctcccgaac 7020
tgggggaagt atgtattact gagtgcaggg gccctgactg ccttgatgtt gataattttc 7080
ctgatgacat gttgtagaag agtcaatcga tcagaaccta cgcaacacaa tctcagaggg 7140
acagggaggg aggtgtcagt cactccccaa agcgggaaga tcatatcttc atgggaatca 7200
cacaagagtg ggggtgagac cagactgtaa ttaattaacg tcctttcaac gatccaagtc 7260
catgaaaaaa actaacaccc ctcccgtacc tagcttataa agtgctgggt catctaagct 7320
tttcagtcga gaaaaaaaca ttagatcaga agaacaactg gcaacacttc tcaacctgag 7380
acttacttca agatgctcga tcctggagag gtctatgatg accctattga cccaatcgag 7440
ttagaggctg aacccagagg aacccccatt gtccccaaca tcttgaggaa ctctgactac 7500
aatctcaact ctcctttgat agaagatcct gctagactaa tgttagaatg gttaaaaaca 7560
gggaatagac cttatcggat gactctaaca gacaattgct ccaggtcttt cagagttttg 7620
aaagattatt tcaagaaggt agatttgggt tctctcaagg tgggcggaat ggctgcacag 7680
tcaatgattt ctctctggtt atatggtgcc cactctgaat ccaacaggag ccggagatgt 7740
ataacagact tggcccattt ctattccaag tcgtccccca tagagaagct gttgaatctc 7800
acgctaggaa atagagggct gagaatcccc ccagagggag tgttaagttg ccttgagagg 7860
gttgattatg ataatgcatt tggaaggtat cttgccaaca cgtattcctc ttacttgttc 7920
ttccatgtaa tcaccttata catgaacgcc ctagactggg atgaagaaaa gaccatccta 7980
gcattatgga aagatttaac ctcagtggac atcgggaagg acttggtaaa gttcaaagac 8040
caaatatggg gactgctgat cgtgacaaag gactttgttt actcccaaag ttccaattgt 8100
ctttttgaca gaaactacac acttatgcta aaagatcttt tcttgtctcg cttcaactcc 8160
ttaatggtct tgctctctcc cccagagccc cgatactcag atgacttgat atctcaacta 8220
tgccagctgt acattgctgg ggatcaagtc ttgtctatgt gtggaaactc cggctatgaa 8280
gtcatcaaaa tattggagcc atatgtcgtg aatagtttag tccagagagc agaaaagttt 8340
aggcctctca ttcattcctt gggagacttt cctgtattta taaaagacaa ggtaagtcaa 8400
cttgaagaga cgttcggtcc ctgtgcaaga aggttcttta gggctctgga tcaattcgac 8460
aacatacatg acttggtttt tgtgtttggc tgttacaggc attgggggca cccatatata 8520
gattatcgaa agggtctgtc aaaactatat gatcaggttc accttaaaaa aatgatagat 8580
aagtcctacc aggagtgctt agcaagcgac ctagccagga ggatccttag atggggtttt 8640
gataagtact ccaagtggta tctggattca agattcctag cccgagacca ccccttgact 8700
ccttatatca aaacccaaac atggccaccc aaacatattg tagacttggt gggggataca 8760
tggcacaagc tcccgatcac gcagatcttt gagattcctg aatcaatgga tccgtcagaa 8820
atattggatg acaaatcaca ttctttcacc agaacgagac tagcttcttg gctgtcagaa 8880
aaccgagggg ggcctgttcc tagcgaaaaa gttattatca cggccctgtc taagccgcct 8940
gtcaatcccc gagagtttct gaggtctata gacctcggag gattgccaga tgaagacttg 9000
ataattggcc tcaagccaaa ggaacgggaa ttgaagattg aaggtcgatt ctttgctcta 9060
atgtcatgga atctaagatt gtattttgtc atcactgaaa aactcttggc caactacatc 9120
ttgccacttt ttgacgcgct gactatgaca gacaacctga acaaggtgtt taaaaagctg 9180
atcgacaggg tcaccgggca agggcttttg gactattcaa gggtcacata tgcatttcac 9240
ctggactatg aaaagtggaa caaccatcaa agattagagt caacagagga tgtattttct 9300
gtcctagatc aagtgtttgg attgaagaga gtgttttcta gaacacacga gttttttcaa 9360
aaggcctgga tctattattc agacagatca gacctcatcg ggttacggga ggatcaaata 9420
tactgcttag atgcgtccaa cggcccaacc tgttggaatg gccaggatgg cgggctagaa 9480
ggcttacggc agaagggctg gagtctagtc agcttattga tgatagatag agaatctcaa 9540
atcaggaaca caagaaccaa aatactagct caaggagaca accaggtttt atgtccgaca 9600
tacatgttgt cgccagggct atctcaagag gggctcctct atgaattgga gagaatatca 9660
aggaatgcac tttcgatata cagagccgtc gaggaagggg catctaagct agggctgatc 9720
atcaagaaag aagagaccat gtgtagttat gacttcctca tctatggaaa aacccctttg 9780
tttagaggta acatattggt gcctgagtcc aaaagatggg ccagagtctc ttgcgtctct 9840
aatgaccaaa tagtcaacct cgccaatata atgtcgacag tgtccaccaa tgcgctaaca 9900
gtggcacaac actctcaatc tttgatcaaa ccgatgaggg attttctgct catgtcagta 9960
caggcagtct ttcactacct gctatttagc ccaatcttaa agggaagagt ttacaagatt 10020
ctgagcgctg aaggggagag ctttctccta gccatgtcaa ggataatcta tctagatcct 10080
tctttgggag ggatatctgg aatgtccctc ggaagattcc atatacgaca gttctcagac 10140
cctgtctctg aagggttatc cttctggaga gagatctggt taagctccca agagtcctgg 10200
attcacgcgt tgtgtcaaga ggctggaaac ccagatcttg gagagagaac actcgagagc 10260
ttcactcgcc ttctagaaga tccgaccacc ttaaatatca gaggaggggc cagtcctacc 10320
attctactca aggatgcaat cagaaaggct ttatatgacg aggtggacaa ggtggaaaat 10380
tcagagtttc gagaggcaat cctgttgtcc aagacccata gagataattt tatactcttc 10440
ttaatatctg ttgagcctct gtttcctcga tttctcagtg agctattcag ttcgtctttt 10500
ttgggaatcc ccgagtcaat cattggattg atacaaaact cccgaacgat aagaaggcag 10560
tttagaaaga gtctctcaaa aactttagaa gaatccttct acaactcaga gatccacggg 10620
attagtcgga tgacccagac acctcagagg gttggggggg tgtggccttg ctcttcagag 10680
agggcagatc tacttaggga gatctcttgg ggaagaaaag tggtaggcac gacagttcct 10740
cacccttctg agatgttggg attacttccc aagtcctcta tttcttgcac ttgtggagca 10800
acaggaggag gcaatcctag agtttctgta tcagtactcc cgtcctttga tcagtcattt 10860
ttttcacgag gccccctaaa gggatacttg ggctcgtcca cctctatgtc gacccagcta 10920
ttccatgcat gggaaaaagt cactaatgtt catgtggtga agagagctct atcgttaaaa 10980
gaatctataa actggttcat tactagagat tccaacttgg ctcaagctct aattaggaac 11040
attatgtctc tgacaggccc tgatttccct ctagaggagg cccctgtctt caaaaggacg 11100
gggtcagcct tgcataggtt caagtctgcc agatacagcg aaggagggta ttcttctgtc 11160
tgcccgaacc tcctctctca tatttctgtt agtacagaca ccatgtctga tttgacccaa 11220
gacgggaaga actacgattt catgttccag ccattgatgc tttatgcaca gacatggaca 11280
tcagagctgg tacagagaga cacaaggcta agagactcta cgtttcattg gcacctccga 11340
tgcaacaggt gtgtgagacc cattgacgac gtgaccctgg agacctctca gatcttcgag 11400
tttccggatg tgtcgaaaag aatatccaga atggtttctg gggctgtgcc tcacttccag 11460
aggcttcccg atatccgtct gagaccagga gattttgaat ctctaagcgg tagagaaaag 11520
tctcaccata tcggatcagc tcaggggctc ttatactcaa tcttagtggc aattcacgac 11580
tcaggataca atgatggaac catcttccct gtcaacatat acggcaaggt ttcccctaga 11640
gactatttga gagggctcgc aaggggagta ttgataggat cctcgatttg cttcttgaca 11700
agaatgacaa atatcaatat taatagacct cttgaattgg tctcaggggt aatctcatat 11760
attctcctga ggctagataa ccatccctcc ttgtacataa tgctcagaga accgtctctt 11820
agaggagaga tattttctat ccctcagaaa atccccgccg cttatccaac cactatgaaa 11880
gaaggcaaca gatcaatctt gtgttatctc caacatgtgc tacgctatga gcgagagata 11940
atcacggcgt ctccagagaa tgactggcta tggatctttt cagactttag aagtgccaaa 12000
atgacgtacc tatccctcat tacttaccag tctcatcttc tactccagag ggttgagaga 12060
aacctatcta agagtatgag agataacctg cgacaattga gttctttgat gaggcaggtg 12120
ctgggcgggc acggagaaga taccttagag tcagacgaca acattcaacg actgctaaaa 12180
gactctttac gaaggacaag atgggtggat caagaggtgc gccatgcagc tagaaccatg 12240
actggagatt acagccccaa caagaaggtg tcccgtaagg taggatgttc agaatgggtc 12300
tgctctgctc aacaggttgc agtctctacc tcagcaaacc cggcccctgt ctcggagctt 12360
gacataaggg ccctctctaa gaggttccag aaccctttga tctcgggctt gagagtggtt 12420
cagtgggcaa ccggtgctca ttataagctt aagcctattc tagatgatct caatgttttc 12480
ccatctctct gccttgtagt tggggacggg tcagggggga tatcaagggc agtcctcaac 12540
atgtttccag atgccaagct tgtgttcaac agtcttttag aggtgaatga cctgatggct 12600
tccggaacac atccactgcc tccttcagca atcatgaggg gaggaaatga tatcgtctcc 12660
agagtgatag atcttgactc aatctgggaa aaaccgtccg acttgagaaa cttggcaacc 12720
tggaaatact tccagtcagt ccaaaagcag gtcaacatgt cctatgacct cattatttgc 12780
gatgcagaag ttactgacat tgcatctatc aaccggatca ccctgttaat gtccgatttt 12840
gcattgtcta tagatggacc actctatttg gtcttcaaaa cttatgggac tatgctagta 12900
aatccaaact acaaggctat tcaacacctg tcaagagcgt tcccctcggt cacagggttt 12960
atcacccaag taacttcgtc tttttcatct gagctctacc tccgattctc caaacgaggg 13020
aagtttttca gagatgctga gtacttgacc tcttccaccc ttcgagaaat gagccttgtg 13080
ttattcaatt gtagcagccc caagagtgag atgcagagag ctcgttcctt gaactatcag 13140
gatcttgtga gaggatttcc tgaagaaatc atatcaaatc cttacaatga gatgatcata 13200
actctgattg acagtgatgt agaatctttt ctagtccaca agatggttga tgatcttgag 13260
ttacagaggg gaactctgtc taaagtggct atcattatag ccatcatgat agttttctcc 13320
aacagagtct tcaacgtttc caaaccccta actgacccct cgttctatcc accgtctgat 13380
cccaaaatcc tgaggcactt caacatatgt tgcagtacta tgatgtatct atctactgct 13440
ttaggtgacg tccctagctt cgcaagactt cacgacctgt ataacagacc tataacttat 13500
tacttcagaa agcaagtcat tcgagggaac gtttatctat cttggagttg gtccaacgac 13560
acctcagtgt tcaaaagggt agcctgtaat tctagcctga gtctgtcatc tcactggatc 13620
aggttgattt acaagatagt gaagactacc agactcgttg gcagcatcaa ggatctatcc 13680
agagaagtgg aaagacacct tcataggtac aacaggtgga tcaccctaga ggatatcaga 13740
tctagatcat ccctactaga ctacagttgc ctgtgaaccg gatactcctg gaagcctgcc 13800
catgctaaga ctcttgtgtg atgtatcttg aaaaaaacaa gatcctaaat ctgaaccttt 13860
ggttgtttga ttgtttttct catttttgtt gtttatttgt taagcgt 13907
<210> 8
<211> 13958
<212> DNA
<213> 人工序列
<220>
<223> RABV载体: Coravax V2-China (RABVG-E51)
<400> 8
acgcttaaca accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa 60
cacccctaca atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt 120
gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa 180
aaagccctgt ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt 240
gtcaggcatg agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc 300
aatgcagttt tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc 360
acgaaaagga gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga 420
agggaattgg gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca 480
tgcgtcctta gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa 540
cactggtaac tataagacaa acattgcaga caggatagag cagatttttg agacagcccc 600
ttttgttaaa atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg 660
gagtactata ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat 720
tgagcatcta tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc 780
aggactggta tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat 840
actatatttc ttccacaaga actttgagga agagataaga agaatgtttg agccagggca 900
ggagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa 960
atctccttat tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg 1020
ctatatgggt caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga 1080
aatgtctgtt ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag 1140
aagattcttc agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac 1200
tgacgtagca ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg 1260
tgaaaccaga agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa 1320
gagatctcac atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc 1380
attcgccgag tttctaaaca agacatattc gagtgactca taacatgaaa aaaactaaca 1440
cccctcccgt acgccaccat gttcgtgttt ctggtgctgc tgcctctggt gagctcccag 1500
tgcgtgaacc tgaccacaag gacccagctg ccccctgcct ataccaattc cttcacacgg 1560
ggcgtgtact atcccgacaa ggtgttccgg agcagcgtgc tgcactccac acaggatctg 1620
tttctgcctt tcttttctaa cgtgacctgg ttccacgcca tccacgtgag cggcaccaat 1680
ggcacaaagc ggttcgacaa tccagtgctg ccctttaacg atggcgtgta cttcgcctcc 1740
accgagaagt ctaacatcat cagaggctgg atctttggca ccacactgga cagcaagaca 1800
cagtccctgc tgatcgtgaa caatgccacc aacgtggtca tcaaggtgtg cgagttccag 1860
ttttgtaatg atccattcct gggcgtgtac tatcacaaga acaataagtc ttggatggag 1920
agcgagtttc gcgtgtattc ctctgccaac aattgcacat ttgagtacgt gtcccagccc 1980
ttcctgatgg acctggaggg caagcagggc aatttcaaga acctgaggga gttcgtgttt 2040
aagaatatcg atggctactt caaaatctac tccaagcaca ccccaatcaa cctggtgcgc 2100
gacctgccac agggcttctc tgccctggag ccactggtgg atctgcccat cggcatcaac 2160
atcacccggt ttcagacact gctggccctg cacagaagct acctgacacc aggcgacagc 2220
tcctctggat ggaccgcagg agcagcagcc tactatgtgg gctatctgca gcccaggacc 2280
ttcctgctga agtacaacga gaatggcacc atcacagacg ccgtggattg cgccctggat 2340
cccctgtctg agaccaagtg tacactgaag agctttaccg tggagaaggg catctatcag 2400
acaagcaatt tcagggtgca gcctaccgag tccatcgtgc gctttcccaa tatcacaaac 2460
ctgtgccctt ttggcgaggt gttcaacgca acccgcttcg ccagcgtgta cgcctggaat 2520
aggaagcgca tctccaactg cgtggccgac tattctgtgc tgtacaacag cgcctccttc 2580
tctaccttta agtgctatgg cgtgagcccc acaaagctga atgacctgtg ctttaccaac 2640
gtgtacgccg attccttcgt gatcaggggc gacgaggtgc gccagatcgc accaggacag 2700
acaggcaaga tcgcagacta caattataag ctgcctgacg atttcaccgg ctgcgtgatc 2760
gcctggaact ctaacaatct ggatagcaaa gtgggcggca actacaatta tctgtaccgg 2820
ctgtttagaa agtctaatct gaagccattc gagagggaca tctccacaga aatctaccag 2880
gccggctcta ccccctgcaa tggcgtggag ggctttaact gttatttccc tctgcagagc 2940
tacggcttcc agccaacaaa cggcgtgggc tatcagccct accgcgtggt ggtgctgtct 3000
tttgagctgc tgcacgcacc tgcaacagtg tgcggaccaa agaagagcac caatctggtg 3060
aagaacaagt gcgtgaactt caacttcaac ggactgaccg gaacaggcgt gctgaccgag 3120
tccaacaaga agttcctgcc ttttcagcag ttcggcaggg acatcgcaga taccacagac 3180
gccgtgcgcg accctcagac cctggagatc ctggacatca caccatgctc cttcggcggc 3240
gtgtctgtga tcacaccagg caccaataca agcaaccagg tggccgtgct gtatcaggac 3300
gtgaattgta ccgaggtgcc agtggcaatc cacgcagatc agctgacccc tacatggcgg 3360
gtgtactcta ccggcagcaa cgtgttccag acaagagccg gatgcctgat cggagcagag 3420
cacgtgaaca atagctatga gtgcgacatc cctatcggcg ccggcatctg tgcctcctac 3480
cagacccaga caaactcccc agaatcaagc gtgattcctc tggtccatcc actggcagat 3540
ccctccacag tgttcaaaga cggagatgag gccgaagact ttgtggaagt ccacctgcct 3600
gatgtgcata accaggtgtc tggcgtcgac ctgggactgc caaattgggg caagtacgtg 3660
ctgctgagtg ctggagcact gactgccctg atgctgatca ttttcctgat gacctgctgt 3720
cggcgcgtga acagaagtga gcccactcag cacaatctgc gaggaaccgg gagagaagtg 3780
tcagtcacac ctcagagcgg gaaaatcatt agtagttggg aatcacataa aagcgggggc 3840
gagaccaggc tgtgagctag ccatgaaaaa aactaacacc cctcctttcg aaccatccca 3900
aacatgagca agatctttgt caatcctagt gctattagag ccggtctggc cgatcttgag 3960
atggctgaag aaactgttga tctgatcaat agaaatatcg aagacaatca ggctcatctc 4020
caaggggaac ccatagaggt ggacaatctc cctgaggata tggggcgact tcacctggat 4080
gatggaaaat cgcccaacca tggtgagata gccaaggtgg gagaaggcaa gtatcgagag 4140
gactttcaga tggatgaagg agaggatcct agcttcctgt tccagtcata cctggaaaat 4200
gttggagtcc aaatagtcag acaaatgagg tcaggagaga gatttctcaa gatatggtca 4260
cagaccgtag aagagattat atcctatgtc gcggtcaact ttcccaaccc tccaggaaag 4320
tcttcagagg ataaatcaac ccagactact ggccgagagc tcaagaagga gacaacaccc 4380
actccttctc agagagaaag ccaatcatcg aaagccagga tggcggctca aattgcttct 4440
ggccctccag cccttgaatg gtcggctacc aatgaagagg atgatctatc agtggaggct 4500
gagatcgctc accagattgc agaaagtttc tccaaaaaat ataagtttcc ctctcgatcc 4560
tcagggatac tcttgtataa ttttgagcaa ttgaaaatga accttgatga tatagttaaa 4620
gaggcaaaaa atgtaccagg tgtgacccgt ttagcccatg acgggtccaa actcccccta 4680
agatgtgtac tgggatgggt cgctttggcc aactctaaga aattccagtt gttagtcgaa 4740
tccgacaagc tgagtaaaat catgcaagat gacttgaatc gctatacatc ttgctaaccg 4800
aacctctccc ctcagtccct ctagacaata aaatccgaga tgtcccaaag tcaacatgaa 4860
aaaaacaggc aacaccactg ataaaatgaa cctcctacgt aagatagtga aaaaccgcag 4920
ggacgaggac actcaaaaat cctctcccgc gtcagcccct ctggatgacg atgacttgtg 4980
gcttccaccc cctgaatacg tcccgctgaa agaacttaca ggcaagaaga acatgaggaa 5040
cttttgtatc aacggaaggg ttaaagtgtg tagcccgaat ggttactcgt tcaggatcct 5100
gcggcacatt ctgaaatcat tcgacgagat atattctggg aatcatagga tgatcgggtt 5160
agtcaaagtg gttattggac tggctttgtc aggatctcca gtccctgagg gcctgaactg 5220
ggtatacaaa ttgaggagaa cctttatctt ccagtgggct gattccaggg gccctcttga 5280
aggggaggag ttggaatact ctcaggagat cacttgggat gatgatactg agttcgtcgg 5340
attgcaaata agagtgattg caaaacagtg tcatatccag ggcagagtct ggtgtatcaa 5400
catgaacccg agagcatgtc aactatggtc tgacatgtct cttcagacac aaaggtccga 5460
agaggacaaa gattcctctc tgcttctaga ataatcagat tatatcccgc aaatttatca 5520
cttgtttacc tctggaggag agaacatatg ggctcaactc caacccttgg gagcaatata 5580
acaaaaaaca tgttatggtg ccattaaacc gctgcatttc atcaaagtca agttgattac 5640
ctttacattt tgatcctctt ggatgtgaaa aaaactatta acatccctca aaagaccccg 5700
ggaaagatgg ttcctcaggc tctcctgttt gtaccccttc tggtttttcc attgtgtttt 5760
gggaaattcc ctatttacac gataccagac aagcttggtc cctggagtcc gattgacata 5820
catcacctca gctgcccaaa caatttggta gtggaggacg aaggatgcac caacctgtca 5880
gggttctcct acatggaact taaagttgga tacatcttag ccataaaagt gaacgggttc 5940
acttgcacag gcgttgtgac ggaggctgaa acctacacta acttcgttgg ttatgtcaca 6000
accacgttca aaagaaagca tttccgccca acaccagatg catgtagagc cgcgtacaac 6060
tggaagatgg ccggtgaccc cagatatgaa gagtctctac acaatccgta ccctgactac 6120
cgctggcttc gaactgtaaa aaccaccaag gagtctctcg ttatcatatc tccaagtgtg 6180
gcagatttgg acccatatga cagatccctt cactcgaggg tcttccctag cgggaagtgc 6240
tcaggagtag cggtgtcttc tacctactgc tccactaacc acgattacac catttggatg 6300
cccgagaatc cgagactagg gatgtcttgt gacattttta ccaatagtag agggaagaga 6360
gcatccaaag ggagtgagac ttgcggcttt gtagatgaaa gaggcctata taagtcttta 6420
aaaggagcat gcaaactcaa gttatgtgga gttctaggac ttagacttat ggatggaaca 6480
tgggtctcga tgcaaacatc aaatgaaacc aaatggtgcc ctcccgataa gttggtgaac 6540
ctgcacgact ttcgctcaga cgaaattgag caccttgttg tagaggagtt ggtcaggaag 6600
agagaggagt gtctggatgc actagagtcc atcatgacaa ccaagtcagt gagtttcaga 6660
cgtctcagtc atttaagaaa acttgtccct gggtttggaa aagcatatac catattcaac 6720
aagaccttga tggaagccga tgctcactac aagtcagtcg agacttggaa tgagatcctc 6780
ccttcaaaag ggtgtttaag agttgggggg aggtgtcatc ctcatgtgaa cggggtgttt 6840
ttcaatggta taatattagg acctgacggc aatgtcttaa tcccagagat gcaatcatcc 6900
ctcctccagc aacatatgga gttgttggaa tcctcggtta tcccccttgt gcaccccctg 6960
gcagacccgt ctaccgtttt caaggacggt gacgaggctg aggattttgt tgaagttcac 7020
cttcccgatg tgcacaatca ggtctcagga gttgacttgg gtctcccgaa ctgggggaag 7080
tatgtattac tgagtgcagg ggccctgact gccttgatgt tgataatttt cctgatgaca 7140
tgttgtagaa gagtcaatcg atcagaacct acgcaacaca atctcagagg gacagggagg 7200
gaggtgtcag tcactcccca aagcgggaag atcatatctt catgggaatc acacaagagt 7260
gggggtgaga ccagactgta attaattaac gtcctttcaa cgatccaagt ccatgaaaaa 7320
aactaacacc cctcccgtac ctagcttata aagtgctggg tcatctaagc ttttcagtcg 7380
agaaaaaaac attagatcag aagaacaact ggcaacactt ctcaacctga gacttacttc 7440
aagatgctcg atcctggaga ggtctatgat gaccctattg acccaatcga gttagaggct 7500
gaacccagag gaacccccat tgtccccaac atcttgagga actctgacta caatctcaac 7560
tctcctttga tagaagatcc tgctagacta atgttagaat ggttaaaaac agggaataga 7620
ccttatcgga tgactctaac agacaattgc tccaggtctt tcagagtttt gaaagattat 7680
ttcaagaagg tagatttggg ttctctcaag gtgggcggaa tggctgcaca gtcaatgatt 7740
tctctctggt tatatggtgc ccactctgaa tccaacagga gccggagatg tataacagac 7800
ttggcccatt tctattccaa gtcgtccccc atagagaagc tgttgaatct cacgctagga 7860
aatagagggc tgagaatccc cccagaggga gtgttaagtt gccttgagag ggttgattat 7920
gataatgcat ttggaaggta tcttgccaac acgtattcct cttacttgtt cttccatgta 7980
atcaccttat acatgaacgc cctagactgg gatgaagaaa agaccatcct agcattatgg 8040
aaagatttaa cctcagtgga catcgggaag gacttggtaa agttcaaaga ccaaatatgg 8100
ggactgctga tcgtgacaaa ggactttgtt tactcccaaa gttccaattg tctttttgac 8160
agaaactaca cacttatgct aaaagatctt ttcttgtctc gcttcaactc cttaatggtc 8220
ttgctctctc ccccagagcc ccgatactca gatgacttga tatctcaact atgccagctg 8280
tacattgctg gggatcaagt cttgtctatg tgtggaaact ccggctatga agtcatcaaa 8340
atattggagc catatgtcgt gaatagttta gtccagagag cagaaaagtt taggcctctc 8400
attcattcct tgggagactt tcctgtattt ataaaagaca aggtaagtca acttgaagag 8460
acgttcggtc cctgtgcaag aaggttcttt agggctctgg atcaattcga caacatacat 8520
gacttggttt ttgtgtttgg ctgttacagg cattgggggc acccatatat agattatcga 8580
aagggtctgt caaaactata tgatcaggtt caccttaaaa aaatgataga taagtcctac 8640
caggagtgct tagcaagcga cctagccagg aggatcctta gatggggttt tgataagtac 8700
tccaagtggt atctggattc aagattccta gcccgagacc accccttgac tccttatatc 8760
aaaacccaaa catggccacc caaacatatt gtagacttgg tgggggatac atggcacaag 8820
ctcccgatca cgcagatctt tgagattcct gaatcaatgg atccgtcaga aatattggat 8880
gacaaatcac attctttcac cagaacgaga ctagcttctt ggctgtcaga aaaccgaggg 8940
gggcctgttc ctagcgaaaa agttattatc acggccctgt ctaagccgcc tgtcaatccc 9000
cgagagtttc tgaggtctat agacctcgga ggattgccag atgaagactt gataattggc 9060
ctcaagccaa aggaacggga attgaagatt gaaggtcgat tctttgctct aatgtcatgg 9120
aatctaagat tgtattttgt catcactgaa aaactcttgg ccaactacat cttgccactt 9180
tttgacgcgc tgactatgac agacaacctg aacaaggtgt ttaaaaagct gatcgacagg 9240
gtcaccgggc aagggctttt ggactattca agggtcacat atgcatttca cctggactat 9300
gaaaagtgga acaaccatca aagattagag tcaacagagg atgtattttc tgtcctagat 9360
caagtgtttg gattgaagag agtgttttct agaacacacg agttttttca aaaggcctgg 9420
atctattatt cagacagatc agacctcatc gggttacggg aggatcaaat atactgctta 9480
gatgcgtcca acggcccaac ctgttggaat ggccaggatg gcgggctaga aggcttacgg 9540
cagaagggct ggagtctagt cagcttattg atgatagata gagaatctca aatcaggaac 9600
acaagaacca aaatactagc tcaaggagac aaccaggttt tatgtccgac atacatgttg 9660
tcgccagggc tatctcaaga ggggctcctc tatgaattgg agagaatatc aaggaatgca 9720
ctttcgatat acagagccgt cgaggaaggg gcatctaagc tagggctgat catcaagaaa 9780
gaagagacca tgtgtagtta tgacttcctc atctatggaa aaaccccttt gtttagaggt 9840
aacatattgg tgcctgagtc caaaagatgg gccagagtct cttgcgtctc taatgaccaa 9900
atagtcaacc tcgccaatat aatgtcgaca gtgtccacca atgcgctaac agtggcacaa 9960
cactctcaat ctttgatcaa accgatgagg gattttctgc tcatgtcagt acaggcagtc 10020
tttcactacc tgctatttag cccaatctta aagggaagag tttacaagat tctgagcgct 10080
gaaggggaga gctttctcct agccatgtca aggataatct atctagatcc ttctttggga 10140
gggatatctg gaatgtccct cggaagattc catatacgac agttctcaga ccctgtctct 10200
gaagggttat ccttctggag agagatctgg ttaagctccc aagagtcctg gattcacgcg 10260
ttgtgtcaag aggctggaaa cccagatctt ggagagagaa cactcgagag cttcactcgc 10320
cttctagaag atccgaccac cttaaatatc agaggagggg ccagtcctac cattctactc 10380
aaggatgcaa tcagaaaggc tttatatgac gaggtggaca aggtggaaaa ttcagagttt 10440
cgagaggcaa tcctgttgtc caagacccat agagataatt ttatactctt cttaatatct 10500
gttgagcctc tgtttcctcg atttctcagt gagctattca gttcgtcttt tttgggaatc 10560
cccgagtcaa tcattggatt gatacaaaac tcccgaacga taagaaggca gtttagaaag 10620
agtctctcaa aaactttaga agaatccttc tacaactcag agatccacgg gattagtcgg 10680
atgacccaga cacctcagag ggttgggggg gtgtggcctt gctcttcaga gagggcagat 10740
ctacttaggg agatctcttg gggaagaaaa gtggtaggca cgacagttcc tcacccttct 10800
gagatgttgg gattacttcc caagtcctct atttcttgca cttgtggagc aacaggagga 10860
ggcaatccta gagtttctgt atcagtactc ccgtcctttg atcagtcatt tttttcacga 10920
ggccccctaa agggatactt gggctcgtcc acctctatgt cgacccagct attccatgca 10980
tgggaaaaag tcactaatgt tcatgtggtg aagagagctc tatcgttaaa agaatctata 11040
aactggttca ttactagaga ttccaacttg gctcaagctc taattaggaa cattatgtct 11100
ctgacaggcc ctgatttccc tctagaggag gcccctgtct tcaaaaggac ggggtcagcc 11160
ttgcataggt tcaagtctgc cagatacagc gaaggagggt attcttctgt ctgcccgaac 11220
ctcctctctc atatttctgt tagtacagac accatgtctg atttgaccca agacgggaag 11280
aactacgatt tcatgttcca gccattgatg ctttatgcac agacatggac atcagagctg 11340
gtacagagag acacaaggct aagagactct acgtttcatt ggcacctccg atgcaacagg 11400
tgtgtgagac ccattgacga cgtgaccctg gagacctctc agatcttcga gtttccggat 11460
gtgtcgaaaa gaatatccag aatggtttct ggggctgtgc ctcacttcca gaggcttccc 11520
gatatccgtc tgagaccagg agattttgaa tctctaagcg gtagagaaaa gtctcaccat 11580
atcggatcag ctcaggggct cttatactca atcttagtgg caattcacga ctcaggatac 11640
aatgatggaa ccatcttccc tgtcaacata tacggcaagg tttcccctag agactatttg 11700
agagggctcg caaggggagt attgatagga tcctcgattt gcttcttgac aagaatgaca 11760
aatatcaata ttaatagacc tcttgaattg gtctcagggg taatctcata tattctcctg 11820
aggctagata accatccctc cttgtacata atgctcagag aaccgtctct tagaggagag 11880
atattttcta tccctcagaa aatccccgcc gcttatccaa ccactatgaa agaaggcaac 11940
agatcaatct tgtgttatct ccaacatgtg ctacgctatg agcgagagat aatcacggcg 12000
tctccagaga atgactggct atggatcttt tcagacttta gaagtgccaa aatgacgtac 12060
ctatccctca ttacttacca gtctcatctt ctactccaga gggttgagag aaacctatct 12120
aagagtatga gagataacct gcgacaattg agttctttga tgaggcaggt gctgggcggg 12180
cacggagaag ataccttaga gtcagacgac aacattcaac gactgctaaa agactcttta 12240
cgaaggacaa gatgggtgga tcaagaggtg cgccatgcag ctagaaccat gactggagat 12300
tacagcccca acaagaaggt gtcccgtaag gtaggatgtt cagaatgggt ctgctctgct 12360
caacaggttg cagtctctac ctcagcaaac ccggcccctg tctcggagct tgacataagg 12420
gccctctcta agaggttcca gaaccctttg atctcgggct tgagagtggt tcagtgggca 12480
accggtgctc attataagct taagcctatt ctagatgatc tcaatgtttt cccatctctc 12540
tgccttgtag ttggggacgg gtcagggggg atatcaaggg cagtcctcaa catgtttcca 12600
gatgccaagc ttgtgttcaa cagtctttta gaggtgaatg acctgatggc ttccggaaca 12660
catccactgc ctccttcagc aatcatgagg ggaggaaatg atatcgtctc cagagtgata 12720
gatcttgact caatctggga aaaaccgtcc gacttgagaa acttggcaac ctggaaatac 12780
ttccagtcag tccaaaagca ggtcaacatg tcctatgacc tcattatttg cgatgcagaa 12840
gttactgaca ttgcatctat caaccggatc accctgttaa tgtccgattt tgcattgtct 12900
atagatggac cactctattt ggtcttcaaa acttatggga ctatgctagt aaatccaaac 12960
tacaaggcta ttcaacacct gtcaagagcg ttcccctcgg tcacagggtt tatcacccaa 13020
gtaacttcgt ctttttcatc tgagctctac ctccgattct ccaaacgagg gaagtttttc 13080
agagatgctg agtacttgac ctcttccacc cttcgagaaa tgagccttgt gttattcaat 13140
tgtagcagcc ccaagagtga gatgcagaga gctcgttcct tgaactatca ggatcttgtg 13200
agaggatttc ctgaagaaat catatcaaat ccttacaatg agatgatcat aactctgatt 13260
gacagtgatg tagaatcttt tctagtccac aagatggttg atgatcttga gttacagagg 13320
ggaactctgt ctaaagtggc tatcattata gccatcatga tagttttctc caacagagtc 13380
ttcaacgttt ccaaacccct aactgacccc tcgttctatc caccgtctga tcccaaaatc 13440
ctgaggcact tcaacatatg ttgcagtact atgatgtatc tatctactgc tttaggtgac 13500
gtccctagct tcgcaagact tcacgacctg tataacagac ctataactta ttacttcaga 13560
aagcaagtca ttcgagggaa cgtttatcta tcttggagtt ggtccaacga cacctcagtg 13620
ttcaaaaggg tagcctgtaa ttctagcctg agtctgtcat ctcactggat caggttgatt 13680
tacaagatag tgaagactac cagactcgtt ggcagcatca aggatctatc cagagaagtg 13740
gaaagacacc ttcataggta caacaggtgg atcaccctag aggatatcag atctagatca 13800
tccctactag actacagttg cctgtgaacc ggatactcct ggaagcctgc ccatgctaag 13860
actcttgtgt gatgtatctt gaaaaaaaca agatcctaaa tctgaacctt tggttgtttg 13920
attgtttttc tcatttttgt tgtttatttg ttaagcgt 13958
<210> 9
<211> 13958
<212> DNA
<213> 人工序列
<220>
<223> RABV载体: Coravax V2 South Africa (S1-RABVG-E51)
<400> 9
acgcttaaca accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa 60
cacccctaca atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt 120
gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa 180
aaagccctgt ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt 240
gtcaggcatg agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc 300
aatgcagttt tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc 360
acgaaaagga gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga 420
agggaattgg gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca 480
tgcgtcctta gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa 540
cactggtaac tataagacaa acattgcaga caggatagag cagatttttg agacagcccc 600
ttttgttaaa atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg 660
gagtactata ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat 720
tgagcatcta tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc 780
aggactggta tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat 840
actatatttc ttccacaaga actttgagga agagataaga agaatgtttg agccagggca 900
ggagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa 960
atctccttat tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg 1020
ctatatgggt caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga 1080
aatgtctgtt ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag 1140
aagattcttc agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac 1200
tgacgtagca ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg 1260
tgaaaccaga agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa 1320
gagatctcac atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc 1380
attcgccgag tttctaaaca agacatattc gagtgactca taacatgaaa aaaactaaca 1440
cccctcccgt acgccaccat gttcgtgttt ctggtgctgc tgcctctggt gagctcccag 1500
tgcgtgaact tcaccacaag gacccagctg ccccctgcct ataccaattc cttcacacgg 1560
ggcgtgtact atcccgacaa ggtgttccgg agcagcgtgc tgcactccac acaggatctg 1620
tttctgcctt tcttttctaa cgtgacctgg ttccacgcca tccacgtgag cggcaccaat 1680
ggcacaaagc ggttcgccaa tccagtgctg ccctttaacg atggcgtgta cttcgcctcc 1740
accgagaagt ctaacatcat cagaggctgg atctttggca ccacactgga cagcaagaca 1800
cagtccctgc tgatcgtgaa caatgccacc aacgtggtca tcaaggtgtg cgagttccag 1860
ttttgtaatg atccattcct gggcgtgtac tatcacaaga acaataagtc ttggatggag 1920
agcgagtttc gcgtgtattc ctctgccaac aattgcacat ttgagtacgt gtcccagccc 1980
ttcctgatgg acctggaggg caagcagggc aatttcaaga acctgaggga gttcgtgttt 2040
aagaatatcg atggctactt caaaatctac tccaagcaca ccccaatcaa cctggtgcgc 2100
ggcctgccac agggcttctc tgccctggag ccactggtgg atctgcccat cggcatcaac 2160
atcacccggt ttcagacact gctggccctg cacagaagct acctgacacc aggcgacagc 2220
tcctctggat ggaccgcagg agcagcagcc tactatgtgg gctatctgca gcccaggacc 2280
ttcctgctga agtacaacga gaatggcacc atcacagacg ccgtggattg cgccctggat 2340
cccctgtctg agaccaagtg tacactgaag agctttaccg tggagaaggg catctatcag 2400
acaagcaatt tcagggtgca gcctaccgag tccatcgtgc gctttcccaa tatcacaaac 2460
ctgtgccctt ttggcgaggt gttcaacgca acccgcttcg ccagcgtgta cgcctggaat 2520
aggaagcgca tctccaactg cgtggccgac tattctgtgc tgtacaacag cgcctccttc 2580
tctaccttta agtgctatgg cgtgagcccc acaaagctga atgacctgtg ctttaccaac 2640
gtgtacgccg attccttcgt gatcaggggc gacgaggtgc gccagatcgc accaggacag 2700
acaggcaata tcgcagacta caattataag ctgcctgacg atttcaccgg ctgcgtgatc 2760
gcctggaact ctaacaatct ggatagcaaa gtgggcggca actacaatta tctgtaccgg 2820
ctgtttagaa agtctaatct gaagccattc gagagggaca tctccacaga aatctaccag 2880
gccggctcta ccccctgcaa tggcgtgaag ggctttaact gttatttccc tctgcagagc 2940
tacggcttcc agccaacata tggcgtgggc tatcagccct accgcgtggt ggtgctgtct 3000
tttgagctgc tgcacgcacc tgcaacagtg tgcggaccaa agaagagcac caatctggtg 3060
aagaacaagt gcgtgaactt caacttcaac ggactgaccg gaacaggcgt gctgaccgag 3120
tccaacaaga agttcctgcc ttttcagcag ttcggcaggg acatcgcaga taccacagac 3180
gccgtgcgcg accctcagac cctggagatc ctggacatca caccatgctc cttcggcggc 3240
gtgtctgtga tcacaccagg caccaataca agcaaccagg tggccgtgct gtatcagggc 3300
gtgaattgta ccgaggtgcc agtggcaatc cacgcagatc agctgacccc tacatggcgg 3360
gtgtactcta ccggcagcaa cgtgttccag acaagagccg gatgcctgat cggagcagag 3420
cacgtgaaca atagctatga gtgcgacatc cctatcggcg ccggcatctg tgcctcctac 3480
cagacccaga caaactcccc agaatcaagc gtgattcctc tggtccatcc actggcagat 3540
ccctccacag tgttcaaaga cggagatgag gccgaagact ttgtggaagt ccacctgcct 3600
gatgtgcata accaggtgtc tggcgtcgac ctgggactgc caaattgggg caagtacgtg 3660
ctgctgagtg ctggagcact gactgccctg atgctgatca ttttcctgat gacctgctgt 3720
cggcgcgtga acagaagtga gcccactcag cacaatctgc gaggaaccgg gagagaagtg 3780
tcagtcacac ctcagagcgg gaaaatcatt agtagttggg aatcacataa aagcgggggc 3840
gagaccaggc tgtgagctag ccatgaaaaa aactaacacc cctcctttcg aaccatccca 3900
aacatgagca agatctttgt caatcctagt gctattagag ccggtctggc cgatcttgag 3960
atggctgaag aaactgttga tctgatcaat agaaatatcg aagacaatca ggctcatctc 4020
caaggggaac ccatagaggt ggacaatctc cctgaggata tggggcgact tcacctggat 4080
gatggaaaat cgcccaacca tggtgagata gccaaggtgg gagaaggcaa gtatcgagag 4140
gactttcaga tggatgaagg agaggatcct agcttcctgt tccagtcata cctggaaaat 4200
gttggagtcc aaatagtcag acaaatgagg tcaggagaga gatttctcaa gatatggtca 4260
cagaccgtag aagagattat atcctatgtc gcggtcaact ttcccaaccc tccaggaaag 4320
tcttcagagg ataaatcaac ccagactact ggccgagagc tcaagaagga gacaacaccc 4380
actccttctc agagagaaag ccaatcatcg aaagccagga tggcggctca aattgcttct 4440
ggccctccag cccttgaatg gtcggctacc aatgaagagg atgatctatc agtggaggct 4500
gagatcgctc accagattgc agaaagtttc tccaaaaaat ataagtttcc ctctcgatcc 4560
tcagggatac tcttgtataa ttttgagcaa ttgaaaatga accttgatga tatagttaaa 4620
gaggcaaaaa atgtaccagg tgtgacccgt ttagcccatg acgggtccaa actcccccta 4680
agatgtgtac tgggatgggt cgctttggcc aactctaaga aattccagtt gttagtcgaa 4740
tccgacaagc tgagtaaaat catgcaagat gacttgaatc gctatacatc ttgctaaccg 4800
aacctctccc ctcagtccct ctagacaata aaatccgaga tgtcccaaag tcaacatgaa 4860
aaaaacaggc aacaccactg ataaaatgaa cctcctacgt aagatagtga aaaaccgcag 4920
ggacgaggac actcaaaaat cctctcccgc gtcagcccct ctggatgacg atgacttgtg 4980
gcttccaccc cctgaatacg tcccgctgaa agaacttaca ggcaagaaga acatgaggaa 5040
cttttgtatc aacggaaggg ttaaagtgtg tagcccgaat ggttactcgt tcaggatcct 5100
gcggcacatt ctgaaatcat tcgacgagat atattctggg aatcatagga tgatcgggtt 5160
agtcaaagtg gttattggac tggctttgtc aggatctcca gtccctgagg gcctgaactg 5220
ggtatacaaa ttgaggagaa cctttatctt ccagtgggct gattccaggg gccctcttga 5280
aggggaggag ttggaatact ctcaggagat cacttgggat gatgatactg agttcgtcgg 5340
attgcaaata agagtgattg caaaacagtg tcatatccag ggcagagtct ggtgtatcaa 5400
catgaacccg agagcatgtc aactatggtc tgacatgtct cttcagacac aaaggtccga 5460
agaggacaaa gattcctctc tgcttctaga ataatcagat tatatcccgc aaatttatca 5520
cttgtttacc tctggaggag agaacatatg ggctcaactc caacccttgg gagcaatata 5580
acaaaaaaca tgttatggtg ccattaaacc gctgcatttc atcaaagtca agttgattac 5640
ctttacattt tgatcctctt ggatgtgaaa aaaactatta acatccctca aaagaccccg 5700
ggaaagatgg ttcctcaggc tctcctgttt gtaccccttc tggtttttcc attgtgtttt 5760
gggaaattcc ctatttacac gataccagac aagcttggtc cctggagtcc gattgacata 5820
catcacctca gctgcccaaa caatttggta gtggaggacg aaggatgcac caacctgtca 5880
gggttctcct acatggaact taaagttgga tacatcttag ccataaaagt gaacgggttc 5940
acttgcacag gcgttgtgac ggaggctgaa acctacacta acttcgttgg ttatgtcaca 6000
accacgttca aaagaaagca tttccgccca acaccagatg catgtagagc cgcgtacaac 6060
tggaagatgg ccggtgaccc cagatatgaa gagtctctac acaatccgta ccctgactac 6120
cgctggcttc gaactgtaaa aaccaccaag gagtctctcg ttatcatatc tccaagtgtg 6180
gcagatttgg acccatatga cagatccctt cactcgaggg tcttccctag cgggaagtgc 6240
tcaggagtag cggtgtcttc tacctactgc tccactaacc acgattacac catttggatg 6300
cccgagaatc cgagactagg gatgtcttgt gacattttta ccaatagtag agggaagaga 6360
gcatccaaag ggagtgagac ttgcggcttt gtagatgaaa gaggcctata taagtcttta 6420
aaaggagcat gcaaactcaa gttatgtgga gttctaggac ttagacttat ggatggaaca 6480
tgggtctcga tgcaaacatc aaatgaaacc aaatggtgcc ctcccgataa gttggtgaac 6540
ctgcacgact ttcgctcaga cgaaattgag caccttgttg tagaggagtt ggtcaggaag 6600
agagaggagt gtctggatgc actagagtcc atcatgacaa ccaagtcagt gagtttcaga 6660
cgtctcagtc atttaagaaa acttgtccct gggtttggaa aagcatatac catattcaac 6720
aagaccttga tggaagccga tgctcactac aagtcagtcg agacttggaa tgagatcctc 6780
ccttcaaaag ggtgtttaag agttgggggg aggtgtcatc ctcatgtgaa cggggtgttt 6840
ttcaatggta taatattagg acctgacggc aatgtcttaa tcccagagat gcaatcatcc 6900
ctcctccagc aacatatgga gttgttggaa tcctcggtta tcccccttgt gcaccccctg 6960
gcagacccgt ctaccgtttt caaggacggt gacgaggctg aggattttgt tgaagttcac 7020
cttcccgatg tgcacaatca ggtctcagga gttgacttgg gtctcccgaa ctgggggaag 7080
tatgtattac tgagtgcagg ggccctgact gccttgatgt tgataatttt cctgatgaca 7140
tgttgtagaa gagtcaatcg atcagaacct acgcaacaca atctcagagg gacagggagg 7200
gaggtgtcag tcactcccca aagcgggaag atcatatctt catgggaatc acacaagagt 7260
gggggtgaga ccagactgta attaattaac gtcctttcaa cgatccaagt ccatgaaaaa 7320
aactaacacc cctcccgtac ctagcttata aagtgctggg tcatctaagc ttttcagtcg 7380
agaaaaaaac attagatcag aagaacaact ggcaacactt ctcaacctga gacttacttc 7440
aagatgctcg atcctggaga ggtctatgat gaccctattg acccaatcga gttagaggct 7500
gaacccagag gaacccccat tgtccccaac atcttgagga actctgacta caatctcaac 7560
tctcctttga tagaagatcc tgctagacta atgttagaat ggttaaaaac agggaataga 7620
ccttatcgga tgactctaac agacaattgc tccaggtctt tcagagtttt gaaagattat 7680
ttcaagaagg tagatttggg ttctctcaag gtgggcggaa tggctgcaca gtcaatgatt 7740
tctctctggt tatatggtgc ccactctgaa tccaacagga gccggagatg tataacagac 7800
ttggcccatt tctattccaa gtcgtccccc atagagaagc tgttgaatct cacgctagga 7860
aatagagggc tgagaatccc cccagaggga gtgttaagtt gccttgagag ggttgattat 7920
gataatgcat ttggaaggta tcttgccaac acgtattcct cttacttgtt cttccatgta 7980
atcaccttat acatgaacgc cctagactgg gatgaagaaa agaccatcct agcattatgg 8040
aaagatttaa cctcagtgga catcgggaag gacttggtaa agttcaaaga ccaaatatgg 8100
ggactgctga tcgtgacaaa ggactttgtt tactcccaaa gttccaattg tctttttgac 8160
agaaactaca cacttatgct aaaagatctt ttcttgtctc gcttcaactc cttaatggtc 8220
ttgctctctc ccccagagcc ccgatactca gatgacttga tatctcaact atgccagctg 8280
tacattgctg gggatcaagt cttgtctatg tgtggaaact ccggctatga agtcatcaaa 8340
atattggagc catatgtcgt gaatagttta gtccagagag cagaaaagtt taggcctctc 8400
attcattcct tgggagactt tcctgtattt ataaaagaca aggtaagtca acttgaagag 8460
acgttcggtc cctgtgcaag aaggttcttt agggctctgg atcaattcga caacatacat 8520
gacttggttt ttgtgtttgg ctgttacagg cattgggggc acccatatat agattatcga 8580
aagggtctgt caaaactata tgatcaggtt caccttaaaa aaatgataga taagtcctac 8640
caggagtgct tagcaagcga cctagccagg aggatcctta gatggggttt tgataagtac 8700
tccaagtggt atctggattc aagattccta gcccgagacc accccttgac tccttatatc 8760
aaaacccaaa catggccacc caaacatatt gtagacttgg tgggggatac atggcacaag 8820
ctcccgatca cgcagatctt tgagattcct gaatcaatgg atccgtcaga aatattggat 8880
gacaaatcac attctttcac cagaacgaga ctagcttctt ggctgtcaga aaaccgaggg 8940
gggcctgttc ctagcgaaaa agttattatc acggccctgt ctaagccgcc tgtcaatccc 9000
cgagagtttc tgaggtctat agacctcgga ggattgccag atgaagactt gataattggc 9060
ctcaagccaa aggaacggga attgaagatt gaaggtcgat tctttgctct aatgtcatgg 9120
aatctaagat tgtattttgt catcactgaa aaactcttgg ccaactacat cttgccactt 9180
tttgacgcgc tgactatgac agacaacctg aacaaggtgt ttaaaaagct gatcgacagg 9240
gtcaccgggc aagggctttt ggactattca agggtcacat atgcatttca cctggactat 9300
gaaaagtgga acaaccatca aagattagag tcaacagagg atgtattttc tgtcctagat 9360
caagtgtttg gattgaagag agtgttttct agaacacacg agttttttca aaaggcctgg 9420
atctattatt cagacagatc agacctcatc gggttacggg aggatcaaat atactgctta 9480
gatgcgtcca acggcccaac ctgttggaat ggccaggatg gcgggctaga aggcttacgg 9540
cagaagggct ggagtctagt cagcttattg atgatagata gagaatctca aatcaggaac 9600
acaagaacca aaatactagc tcaaggagac aaccaggttt tatgtccgac atacatgttg 9660
tcgccagggc tatctcaaga ggggctcctc tatgaattgg agagaatatc aaggaatgca 9720
ctttcgatat acagagccgt cgaggaaggg gcatctaagc tagggctgat catcaagaaa 9780
gaagagacca tgtgtagtta tgacttcctc atctatggaa aaaccccttt gtttagaggt 9840
aacatattgg tgcctgagtc caaaagatgg gccagagtct cttgcgtctc taatgaccaa 9900
atagtcaacc tcgccaatat aatgtcgaca gtgtccacca atgcgctaac agtggcacaa 9960
cactctcaat ctttgatcaa accgatgagg gattttctgc tcatgtcagt acaggcagtc 10020
tttcactacc tgctatttag cccaatctta aagggaagag tttacaagat tctgagcgct 10080
gaaggggaga gctttctcct agccatgtca aggataatct atctagatcc ttctttggga 10140
gggatatctg gaatgtccct cggaagattc catatacgac agttctcaga ccctgtctct 10200
gaagggttat ccttctggag agagatctgg ttaagctccc aagagtcctg gattcacgcg 10260
ttgtgtcaag aggctggaaa cccagatctt ggagagagaa cactcgagag cttcactcgc 10320
cttctagaag atccgaccac cttaaatatc agaggagggg ccagtcctac cattctactc 10380
aaggatgcaa tcagaaaggc tttatatgac gaggtggaca aggtggaaaa ttcagagttt 10440
cgagaggcaa tcctgttgtc caagacccat agagataatt ttatactctt cttaatatct 10500
gttgagcctc tgtttcctcg atttctcagt gagctattca gttcgtcttt tttgggaatc 10560
cccgagtcaa tcattggatt gatacaaaac tcccgaacga taagaaggca gtttagaaag 10620
agtctctcaa aaactttaga agaatccttc tacaactcag agatccacgg gattagtcgg 10680
atgacccaga cacctcagag ggttgggggg gtgtggcctt gctcttcaga gagggcagat 10740
ctacttaggg agatctcttg gggaagaaaa gtggtaggca cgacagttcc tcacccttct 10800
gagatgttgg gattacttcc caagtcctct atttcttgca cttgtggagc aacaggagga 10860
ggcaatccta gagtttctgt atcagtactc ccgtcctttg atcagtcatt tttttcacga 10920
ggccccctaa agggatactt gggctcgtcc acctctatgt cgacccagct attccatgca 10980
tgggaaaaag tcactaatgt tcatgtggtg aagagagctc tatcgttaaa agaatctata 11040
aactggttca ttactagaga ttccaacttg gctcaagctc taattaggaa cattatgtct 11100
ctgacaggcc ctgatttccc tctagaggag gcccctgtct tcaaaaggac ggggtcagcc 11160
ttgcataggt tcaagtctgc cagatacagc gaaggagggt attcttctgt ctgcccgaac 11220
ctcctctctc atatttctgt tagtacagac accatgtctg atttgaccca agacgggaag 11280
aactacgatt tcatgttcca gccattgatg ctttatgcac agacatggac atcagagctg 11340
gtacagagag acacaaggct aagagactct acgtttcatt ggcacctccg atgcaacagg 11400
tgtgtgagac ccattgacga cgtgaccctg gagacctctc agatcttcga gtttccggat 11460
gtgtcgaaaa gaatatccag aatggtttct ggggctgtgc ctcacttcca gaggcttccc 11520
gatatccgtc tgagaccagg agattttgaa tctctaagcg gtagagaaaa gtctcaccat 11580
atcggatcag ctcaggggct cttatactca atcttagtgg caattcacga ctcaggatac 11640
aatgatggaa ccatcttccc tgtcaacata tacggcaagg tttcccctag agactatttg 11700
agagggctcg caaggggagt attgatagga tcctcgattt gcttcttgac aagaatgaca 11760
aatatcaata ttaatagacc tcttgaattg gtctcagggg taatctcata tattctcctg 11820
aggctagata accatccctc cttgtacata atgctcagag aaccgtctct tagaggagag 11880
atattttcta tccctcagaa aatccccgcc gcttatccaa ccactatgaa agaaggcaac 11940
agatcaatct tgtgttatct ccaacatgtg ctacgctatg agcgagagat aatcacggcg 12000
tctccagaga atgactggct atggatcttt tcagacttta gaagtgccaa aatgacgtac 12060
ctatccctca ttacttacca gtctcatctt ctactccaga gggttgagag aaacctatct 12120
aagagtatga gagataacct gcgacaattg agttctttga tgaggcaggt gctgggcggg 12180
cacggagaag ataccttaga gtcagacgac aacattcaac gactgctaaa agactcttta 12240
cgaaggacaa gatgggtgga tcaagaggtg cgccatgcag ctagaaccat gactggagat 12300
tacagcccca acaagaaggt gtcccgtaag gtaggatgtt cagaatgggt ctgctctgct 12360
caacaggttg cagtctctac ctcagcaaac ccggcccctg tctcggagct tgacataagg 12420
gccctctcta agaggttcca gaaccctttg atctcgggct tgagagtggt tcagtgggca 12480
accggtgctc attataagct taagcctatt ctagatgatc tcaatgtttt cccatctctc 12540
tgccttgtag ttggggacgg gtcagggggg atatcaaggg cagtcctcaa catgtttcca 12600
gatgccaagc ttgtgttcaa cagtctttta gaggtgaatg acctgatggc ttccggaaca 12660
catccactgc ctccttcagc aatcatgagg ggaggaaatg atatcgtctc cagagtgata 12720
gatcttgact caatctggga aaaaccgtcc gacttgagaa acttggcaac ctggaaatac 12780
ttccagtcag tccaaaagca ggtcaacatg tcctatgacc tcattatttg cgatgcagaa 12840
gttactgaca ttgcatctat caaccggatc accctgttaa tgtccgattt tgcattgtct 12900
atagatggac cactctattt ggtcttcaaa acttatggga ctatgctagt aaatccaaac 12960
tacaaggcta ttcaacacct gtcaagagcg ttcccctcgg tcacagggtt tatcacccaa 13020
gtaacttcgt ctttttcatc tgagctctac ctccgattct ccaaacgagg gaagtttttc 13080
agagatgctg agtacttgac ctcttccacc cttcgagaaa tgagccttgt gttattcaat 13140
tgtagcagcc ccaagagtga gatgcagaga gctcgttcct tgaactatca ggatcttgtg 13200
agaggatttc ctgaagaaat catatcaaat ccttacaatg agatgatcat aactctgatt 13260
gacagtgatg tagaatcttt tctagtccac aagatggttg atgatcttga gttacagagg 13320
ggaactctgt ctaaagtggc tatcattata gccatcatga tagttttctc caacagagtc 13380
ttcaacgttt ccaaacccct aactgacccc tcgttctatc caccgtctga tcccaaaatc 13440
ctgaggcact tcaacatatg ttgcagtact atgatgtatc tatctactgc tttaggtgac 13500
gtccctagct tcgcaagact tcacgacctg tataacagac ctataactta ttacttcaga 13560
aagcaagtca ttcgagggaa cgtttatcta tcttggagtt ggtccaacga cacctcagtg 13620
ttcaaaaggg tagcctgtaa ttctagcctg agtctgtcat ctcactggat caggttgatt 13680
tacaagatag tgaagactac cagactcgtt ggcagcatca aggatctatc cagagaagtg 13740
gaaagacacc ttcataggta caacaggtgg atcaccctag aggatatcag atctagatca 13800
tccctactag actacagttg cctgtgaacc ggatactcct ggaagcctgc ccatgctaag 13860
actcttgtgt gatgtatctt gaaaaaaaca agatcctaaa tctgaacctt tggttgtttg 13920
attgtttttc tcatttttgt tgtttatttg ttaagcgt 13958
<210> 10
<211> 13826
<212> DNA
<213> 人工序列
<220>
<223> RABV载体: Coravax V3-China (S1-VSVG-E26)
<400> 10
acgcttaaca accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa 60
cacccctaca atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt 120
gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa 180
aaagccctgt ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt 240
gtcaggcatg agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc 300
aatgcagttt tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc 360
acgaaaagga gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga 420
agggaattgg gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca 480
tgcgtcctta gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa 540
cactggtaac tataagacaa acattgcaga caggatagag cagatttttg agacagcccc 600
ttttgttaaa atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg 660
gagtactata ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat 720
tgagcatcta tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc 780
aggactggta tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat 840
actatatttc ttccacaaga actttgagga agagataaga agaatgtttg agccagggca 900
ggagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa 960
atctccttat tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg 1020
ctatatgggt caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga 1080
aatgtctgtt ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag 1140
aagattcttc agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac 1200
tgacgtagca ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg 1260
tgaaaccaga agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa 1320
gagatctcac atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc 1380
attcgccgag tttctaaaca agacatattc gagtgactca taacatgaaa aaaactaaca 1440
cccctcccgt acgccaccat gttcgtgttt ctggtgctgc tgcctctggt gagctcccag 1500
tgcgtgaacc tgaccacaag gacccagctg ccccctgcct ataccaattc cttcacacgg 1560
ggcgtgtact atcccgacaa ggtgttccgg agcagcgtgc tgcactccac acaggatctg 1620
tttctgcctt tcttttctaa cgtgacctgg ttccacgcca tccacgtgag cggcaccaat 1680
ggcacaaagc ggttcgacaa tccagtgctg ccctttaacg atggcgtgta cttcgcctcc 1740
accgagaagt ctaacatcat cagaggctgg atctttggca ccacactgga cagcaagaca 1800
cagtccctgc tgatcgtgaa caatgccacc aacgtggtca tcaaggtgtg cgagttccag 1860
ttttgtaatg atccattcct gggcgtgtac tatcacaaga acaataagtc ttggatggag 1920
agcgagtttc gcgtgtattc ctctgccaac aattgcacat ttgagtacgt gtcccagccc 1980
ttcctgatgg acctggaggg caagcagggc aatttcaaga acctgaggga gttcgtgttt 2040
aagaatatcg atggctactt caaaatctac tccaagcaca ccccaatcaa cctggtgcgc 2100
gacctgccac agggcttctc tgccctggag ccactggtgg atctgcccat cggcatcaac 2160
atcacccggt ttcagacact gctggccctg cacagaagct acctgacacc aggcgacagc 2220
tcctctggat ggaccgcagg agcagcagcc tactatgtgg gctatctgca gcccaggacc 2280
ttcctgctga agtacaacga gaatggcacc atcacagacg ccgtggattg cgccctggat 2340
cccctgtctg agaccaagtg tacactgaag agctttaccg tggagaaggg catctatcag 2400
acaagcaatt tcagggtgca gcctaccgag tccatcgtgc gctttcccaa tatcacaaac 2460
ctgtgccctt ttggcgaggt gttcaacgca acccgcttcg ccagcgtgta cgcctggaat 2520
aggaagcgca tctccaactg cgtggccgac tattctgtgc tgtacaacag cgcctccttc 2580
tctaccttta agtgctatgg cgtgagcccc acaaagctga atgacctgtg ctttaccaac 2640
gtgtacgccg attccttcgt gatcaggggc gacgaggtgc gccagatcgc accaggacag 2700
acaggcaaga tcgcagacta caattataag ctgcctgacg atttcaccgg ctgcgtgatc 2760
gcctggaact ctaacaatct ggatagcaaa gtgggcggca actacaatta tctgtaccgg 2820
ctgtttagaa agtctaatct gaagccattc gagagggaca tctccacaga aatctaccag 2880
gccggctcta ccccctgcaa tggcgtggag ggctttaact gttatttccc tctgcagagc 2940
tacggcttcc agccaacaaa cggcgtgggc tatcagccct accgcgtggt ggtgctgtct 3000
tttgagctgc tgcacgcacc tgcaacagtg tgcggaccaa agaagagcac caatctggtg 3060
aagaacaagt gcgtgaactt caacttcaac ggactgaccg gaacaggcgt gctgaccgag 3120
tccaacaaga agttcctgcc ttttcagcag ttcggcaggg acatcgcaga taccacagac 3180
gccgtgcgcg accctcagac cctggagatc ctggacatca caccatgctc cttcggcggc 3240
gtgtctgtga tcacaccagg caccaataca agcaaccagg tggccgtgct gtatcaggac 3300
gtgaattgta ccgaggtgcc agtggcaatc cacgcagatc agctgacccc tacatggcgg 3360
gtgtactcta ccggcagcaa cgtgttccag acaagagccg gatgcctgat cggagcagag 3420
cacgtgaaca atagctatga gtgcgacatc cctatcggcg ccggcatctg tgcctcctac 3480
cagacccaga caaactcccc aaggtctgtg ggcgatacag gcctgtccaa gaatccaatc 3540
gagctggtag agggctggtt cagcagttgg aaaagctcca tcgcctcctt tttctttatc 3600
atcggcctga tcatcggact gttcctggtg ctccgcgtgg gtatccacct gtgcatcaag 3660
ctgaagcaca ccaagaaaag acagatttat acagacatcg agatgaaccg cctgggaaag 3720
tgagctagcc atgaaaaaaa ctaacacccc tcctttcgaa ccatcccaaa catgagcaag 3780
atctttgtca atcctagtgc tattagagcc ggtctggccg atcttgagat ggctgaagaa 3840
actgttgatc tgatcaatag aaatatcgaa gacaatcagg ctcatctcca aggggaaccc 3900
atagaggtgg acaatctccc tgaggatatg gggcgacttc acctggatga tggaaaatcg 3960
cccaaccatg gtgagatagc caaggtggga gaaggcaagt atcgagagga ctttcagatg 4020
gatgaaggag aggatcctag cttcctgttc cagtcatacc tggaaaatgt tggagtccaa 4080
atagtcagac aaatgaggtc aggagagaga tttctcaaga tatggtcaca gaccgtagaa 4140
gagattatat cctatgtcgc ggtcaacttt cccaaccctc caggaaagtc ttcagaggat 4200
aaatcaaccc agactactgg ccgagagctc aagaaggaga caacacccac tccttctcag 4260
agagaaagcc aatcatcgaa agccaggatg gcggctcaaa ttgcttctgg ccctccagcc 4320
cttgaatggt cggctaccaa tgaagaggat gatctatcag tggaggctga gatcgctcac 4380
cagattgcag aaagtttctc caaaaaatat aagtttccct ctcgatcctc agggatactc 4440
ttgtataatt ttgagcaatt gaaaatgaac cttgatgata tagttaaaga ggcaaaaaat 4500
gtaccaggtg tgacccgttt agcccatgac gggtccaaac tccccctaag atgtgtactg 4560
ggatgggtcg ctttggccaa ctctaagaaa ttccagttgt tagtcgaatc cgacaagctg 4620
agtaaaatca tgcaagatga cttgaatcgc tatacatctt gctaaccgaa cctctcccct 4680
cagtccctct agacaataaa atccgagatg tcccaaagtc aacatgaaaa aaacaggcaa 4740
caccactgat aaaatgaacc tcctacgtaa gatagtgaaa aaccgcaggg acgaggacac 4800
tcaaaaatcc tctcccgcgt cagcccctct ggatgacgat gacttgtggc ttccaccccc 4860
tgaatacgtc ccgctgaaag aacttacagg caagaagaac atgaggaact tttgtatcaa 4920
cggaagggtt aaagtgtgta gcccgaatgg ttactcgttc aggatcctgc ggcacattct 4980
gaaatcattc gacgagatat attctgggaa tcataggatg atcgggttag tcaaagtggt 5040
tattggactg gctttgtcag gatctccagt ccctgagggc ctgaactggg tatacaaatt 5100
gaggagaacc tttatcttcc agtgggctga ttccaggggc cctcttgaag gggaggagtt 5160
ggaatactct caggagatca cttgggatga tgatactgag ttcgtcggat tgcaaataag 5220
agtgattgca aaacagtgtc atatccaggg cagagtctgg tgtatcaaca tgaacccgag 5280
agcatgtcaa ctatggtctg acatgtctct tcagacacaa aggtccgaag aggacaaaga 5340
ttcctctctg cttctagaat aatcagatta tatcccgcaa atttatcact tgtttacctc 5400
tggaggagag aacatatggg ctcaactcca acccttggga gcaatataac aaaaaacatg 5460
ttatggtgcc attaaaccgc tgcatttcat caaagtcaag ttgattacct ttacattttg 5520
atcctcttgg atgtgaaaaa aactattaac atccctcaaa agaccccggg aaagatggtt 5580
cctcaggctc tcctgtttgt accccttctg gtttttccat tgtgttttgg gaaattccct 5640
atttacacga taccagacaa gcttggtccc tggagtccga ttgacataca tcacctcagc 5700
tgcccaaaca atttggtagt ggaggacgaa ggatgcacca acctgtcagg gttctcctac 5760
atggaactta aagttggata catcttagcc ataaaagtga acgggttcac ttgcacaggc 5820
gttgtgacgg aggctgaaac ctacactaac ttcgttggtt atgtcacaac cacgttcaaa 5880
agaaagcatt tccgcccaac accagatgca tgtagagccg cgtacaactg gaagatggcc 5940
ggtgacccca gatatgaaga gtctctacac aatccgtacc ctgactaccg ctggcttcga 6000
actgtaaaaa ccaccaagga gtctctcgtt atcatatctc caagtgtggc agatttggac 6060
ccatatgaca gatcccttca ctcgagggtc ttccctagcg ggaagtgctc aggagtagcg 6120
gtgtcttcta cctactgctc cactaaccac gattacacca tttggatgcc cgagaatccg 6180
agactaggga tgtcttgtga catttttacc aatagtagag ggaagagagc atccaaaggg 6240
agtgagactt gcggctttgt agatgaaaga ggcctatata agtctttaaa aggagcatgc 6300
aaactcaagt tatgtggagt tctaggactt agacttatgg atggaacatg ggtctcgatg 6360
caaacatcaa atgaaaccaa atggtgccct cccgataagt tggtgaacct gcacgacttt 6420
cgctcagacg aaattgagca ccttgttgta gaggagttgg tcaggaagag agaggagtgt 6480
ctggatgcac tagagtccat catgacaacc aagtcagtga gtttcagacg tctcagtcat 6540
ttaagaaaac ttgtccctgg gtttggaaaa gcatatacca tattcaacaa gaccttgatg 6600
gaagccgatg ctcactacaa gtcagtcgag acttggaatg agatcctccc ttcaaaaggg 6660
tgtttaagag ttggggggag gtgtcatcct catgtgaacg gggtgttttt caatggtata 6720
atattaggac ctgacggcaa tgtcttaatc ccagagatgc aatcatccct cctccagcaa 6780
catatggagt tgttggaatc ctcggttatc ccccttgtgc accccctggc agacccgtct 6840
accgttttca aggacggtga cgaggctgag gattttgttg aagttcacct tcccgatgtg 6900
cacaatcagg tctcaggagt tgacttgggt ctcccgaact gggggaagta tgtattactg 6960
agtgcagggg ccctgactgc cttgatgttg ataattttcc tgatgacatg ttgtagaaga 7020
gtcaatcgat cagaacctac gcaacacaat ctcagaggga cagggaggga ggtgtcagtc 7080
actccccaaa gcgggaagat catatcttca tgggaatcac acaagagtgg gggtgagacc 7140
agactgtaat taattaacgt cctttcaacg atccaagtcc atgaaaaaaa ctaacacccc 7200
tcccgtacct agcttataaa gtgctgggtc atctaagctt ttcagtcgag aaaaaaacat 7260
tagatcagaa gaacaactgg caacacttct caacctgaga cttacttcaa gatgctcgat 7320
cctggagagg tctatgatga ccctattgac ccaatcgagt tagaggctga acccagagga 7380
acccccattg tccccaacat cttgaggaac tctgactaca atctcaactc tcctttgata 7440
gaagatcctg ctagactaat gttagaatgg ttaaaaacag ggaatagacc ttatcggatg 7500
actctaacag acaattgctc caggtctttc agagttttga aagattattt caagaaggta 7560
gatttgggtt ctctcaaggt gggcggaatg gctgcacagt caatgatttc tctctggtta 7620
tatggtgccc actctgaatc caacaggagc cggagatgta taacagactt ggcccatttc 7680
tattccaagt cgtcccccat agagaagctg ttgaatctca cgctaggaaa tagagggctg 7740
agaatccccc cagagggagt gttaagttgc cttgagaggg ttgattatga taatgcattt 7800
ggaaggtatc ttgccaacac gtattcctct tacttgttct tccatgtaat caccttatac 7860
atgaacgccc tagactggga tgaagaaaag accatcctag cattatggaa agatttaacc 7920
tcagtggaca tcgggaagga cttggtaaag ttcaaagacc aaatatgggg actgctgatc 7980
gtgacaaagg actttgttta ctcccaaagt tccaattgtc tttttgacag aaactacaca 8040
cttatgctaa aagatctttt cttgtctcgc ttcaactcct taatggtctt gctctctccc 8100
ccagagcccc gatactcaga tgacttgata tctcaactat gccagctgta cattgctggg 8160
gatcaagtct tgtctatgtg tggaaactcc ggctatgaag tcatcaaaat attggagcca 8220
tatgtcgtga atagtttagt ccagagagca gaaaagttta ggcctctcat tcattccttg 8280
ggagactttc ctgtatttat aaaagacaag gtaagtcaac ttgaagagac gttcggtccc 8340
tgtgcaagaa ggttctttag ggctctggat caattcgaca acatacatga cttggttttt 8400
gtgtttggct gttacaggca ttgggggcac ccatatatag attatcgaaa gggtctgtca 8460
aaactatatg atcaggttca ccttaaaaaa atgatagata agtcctacca ggagtgctta 8520
gcaagcgacc tagccaggag gatccttaga tggggttttg ataagtactc caagtggtat 8580
ctggattcaa gattcctagc ccgagaccac cccttgactc cttatatcaa aacccaaaca 8640
tggccaccca aacatattgt agacttggtg ggggatacat ggcacaagct cccgatcacg 8700
cagatctttg agattcctga atcaatggat ccgtcagaaa tattggatga caaatcacat 8760
tctttcacca gaacgagact agcttcttgg ctgtcagaaa accgaggggg gcctgttcct 8820
agcgaaaaag ttattatcac ggccctgtct aagccgcctg tcaatccccg agagtttctg 8880
aggtctatag acctcggagg attgccagat gaagacttga taattggcct caagccaaag 8940
gaacgggaat tgaagattga aggtcgattc tttgctctaa tgtcatggaa tctaagattg 9000
tattttgtca tcactgaaaa actcttggcc aactacatct tgccactttt tgacgcgctg 9060
actatgacag acaacctgaa caaggtgttt aaaaagctga tcgacagggt caccgggcaa 9120
gggcttttgg actattcaag ggtcacatat gcatttcacc tggactatga aaagtggaac 9180
aaccatcaaa gattagagtc aacagaggat gtattttctg tcctagatca agtgtttgga 9240
ttgaagagag tgttttctag aacacacgag ttttttcaaa aggcctggat ctattattca 9300
gacagatcag acctcatcgg gttacgggag gatcaaatat actgcttaga tgcgtccaac 9360
ggcccaacct gttggaatgg ccaggatggc gggctagaag gcttacggca gaagggctgg 9420
agtctagtca gcttattgat gatagataga gaatctcaaa tcaggaacac aagaaccaaa 9480
atactagctc aaggagacaa ccaggtttta tgtccgacat acatgttgtc gccagggcta 9540
tctcaagagg ggctcctcta tgaattggag agaatatcaa ggaatgcact ttcgatatac 9600
agagccgtcg aggaaggggc atctaagcta gggctgatca tcaagaaaga agagaccatg 9660
tgtagttatg acttcctcat ctatggaaaa acccctttgt ttagaggtaa catattggtg 9720
cctgagtcca aaagatgggc cagagtctct tgcgtctcta atgaccaaat agtcaacctc 9780
gccaatataa tgtcgacagt gtccaccaat gcgctaacag tggcacaaca ctctcaatct 9840
ttgatcaaac cgatgaggga ttttctgctc atgtcagtac aggcagtctt tcactacctg 9900
ctatttagcc caatcttaaa gggaagagtt tacaagattc tgagcgctga aggggagagc 9960
tttctcctag ccatgtcaag gataatctat ctagatcctt ctttgggagg gatatctgga 10020
atgtccctcg gaagattcca tatacgacag ttctcagacc ctgtctctga agggttatcc 10080
ttctggagag agatctggtt aagctcccaa gagtcctgga ttcacgcgtt gtgtcaagag 10140
gctggaaacc cagatcttgg agagagaaca ctcgagagct tcactcgcct tctagaagat 10200
ccgaccacct taaatatcag aggaggggcc agtcctacca ttctactcaa ggatgcaatc 10260
agaaaggctt tatatgacga ggtggacaag gtggaaaatt cagagtttcg agaggcaatc 10320
ctgttgtcca agacccatag agataatttt atactcttct taatatctgt tgagcctctg 10380
tttcctcgat ttctcagtga gctattcagt tcgtcttttt tgggaatccc cgagtcaatc 10440
attggattga tacaaaactc ccgaacgata agaaggcagt ttagaaagag tctctcaaaa 10500
actttagaag aatccttcta caactcagag atccacggga ttagtcggat gacccagaca 10560
cctcagaggg ttgggggggt gtggccttgc tcttcagaga gggcagatct acttagggag 10620
atctcttggg gaagaaaagt ggtaggcacg acagttcctc acccttctga gatgttggga 10680
ttacttccca agtcctctat ttcttgcact tgtggagcaa caggaggagg caatcctaga 10740
gtttctgtat cagtactccc gtcctttgat cagtcatttt tttcacgagg ccccctaaag 10800
ggatacttgg gctcgtccac ctctatgtcg acccagctat tccatgcatg ggaaaaagtc 10860
actaatgttc atgtggtgaa gagagctcta tcgttaaaag aatctataaa ctggttcatt 10920
actagagatt ccaacttggc tcaagctcta attaggaaca ttatgtctct gacaggccct 10980
gatttccctc tagaggaggc ccctgtcttc aaaaggacgg ggtcagcctt gcataggttc 11040
aagtctgcca gatacagcga aggagggtat tcttctgtct gcccgaacct cctctctcat 11100
atttctgtta gtacagacac catgtctgat ttgacccaag acgggaagaa ctacgatttc 11160
atgttccagc cattgatgct ttatgcacag acatggacat cagagctggt acagagagac 11220
acaaggctaa gagactctac gtttcattgg cacctccgat gcaacaggtg tgtgagaccc 11280
attgacgacg tgaccctgga gacctctcag atcttcgagt ttccggatgt gtcgaaaaga 11340
atatccagaa tggtttctgg ggctgtgcct cacttccaga ggcttcccga tatccgtctg 11400
agaccaggag attttgaatc tctaagcggt agagaaaagt ctcaccatat cggatcagct 11460
caggggctct tatactcaat cttagtggca attcacgact caggatacaa tgatggaacc 11520
atcttccctg tcaacatata cggcaaggtt tcccctagag actatttgag agggctcgca 11580
aggggagtat tgataggatc ctcgatttgc ttcttgacaa gaatgacaaa tatcaatatt 11640
aatagacctc ttgaattggt ctcaggggta atctcatata ttctcctgag gctagataac 11700
catccctcct tgtacataat gctcagagaa ccgtctctta gaggagagat attttctatc 11760
cctcagaaaa tccccgccgc ttatccaacc actatgaaag aaggcaacag atcaatcttg 11820
tgttatctcc aacatgtgct acgctatgag cgagagataa tcacggcgtc tccagagaat 11880
gactggctat ggatcttttc agactttaga agtgccaaaa tgacgtacct atccctcatt 11940
acttaccagt ctcatcttct actccagagg gttgagagaa acctatctaa gagtatgaga 12000
gataacctgc gacaattgag ttctttgatg aggcaggtgc tgggcgggca cggagaagat 12060
accttagagt cagacgacaa cattcaacga ctgctaaaag actctttacg aaggacaaga 12120
tgggtggatc aagaggtgcg ccatgcagct agaaccatga ctggagatta cagccccaac 12180
aagaaggtgt cccgtaaggt aggatgttca gaatgggtct gctctgctca acaggttgca 12240
gtctctacct cagcaaaccc ggcccctgtc tcggagcttg acataagggc cctctctaag 12300
aggttccaga accctttgat ctcgggcttg agagtggttc agtgggcaac cggtgctcat 12360
tataagctta agcctattct agatgatctc aatgttttcc catctctctg ccttgtagtt 12420
ggggacgggt caggggggat atcaagggca gtcctcaaca tgtttccaga tgccaagctt 12480
gtgttcaaca gtcttttaga ggtgaatgac ctgatggctt ccggaacaca tccactgcct 12540
ccttcagcaa tcatgagggg aggaaatgat atcgtctcca gagtgataga tcttgactca 12600
atctgggaaa aaccgtccga cttgagaaac ttggcaacct ggaaatactt ccagtcagtc 12660
caaaagcagg tcaacatgtc ctatgacctc attatttgcg atgcagaagt tactgacatt 12720
gcatctatca accggatcac cctgttaatg tccgattttg cattgtctat agatggacca 12780
ctctatttgg tcttcaaaac ttatgggact atgctagtaa atccaaacta caaggctatt 12840
caacacctgt caagagcgtt cccctcggtc acagggttta tcacccaagt aacttcgtct 12900
ttttcatctg agctctacct ccgattctcc aaacgaggga agtttttcag agatgctgag 12960
tacttgacct cttccaccct tcgagaaatg agccttgtgt tattcaattg tagcagcccc 13020
aagagtgaga tgcagagagc tcgttccttg aactatcagg atcttgtgag aggatttcct 13080
gaagaaatca tatcaaatcc ttacaatgag atgatcataa ctctgattga cagtgatgta 13140
gaatcttttc tagtccacaa gatggttgat gatcttgagt tacagagggg aactctgtct 13200
aaagtggcta tcattatagc catcatgata gttttctcca acagagtctt caacgtttcc 13260
aaacccctaa ctgacccctc gttctatcca ccgtctgatc ccaaaatcct gaggcacttc 13320
aacatatgtt gcagtactat gatgtatcta tctactgctt taggtgacgt ccctagcttc 13380
gcaagacttc acgacctgta taacagacct ataacttatt acttcagaaa gcaagtcatt 13440
cgagggaacg tttatctatc ttggagttgg tccaacgaca cctcagtgtt caaaagggta 13500
gcctgtaatt ctagcctgag tctgtcatct cactggatca ggttgattta caagatagtg 13560
aagactacca gactcgttgg cagcatcaag gatctatcca gagaagtgga aagacacctt 13620
cataggtaca acaggtggat caccctagag gatatcagat ctagatcatc cctactagac 13680
tacagttgcc tgtgaaccgg atactcctgg aagcctgccc atgctaagac tcttgtgtga 13740
tgtatcttga aaaaaacaag atcctaaatc tgaacctttg gttgtttgat tgtttttctc 13800
atttttgttg tttatttgtt aagcgt 13826
<210> 11
<211> 13826
<212> DNA
<213> 人工序列
<220>
<223> RABV载体: Coravax V3-South Africa (S1-VSVG-E26)
<400> 11
acgcttaaca accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa 60
cacccctaca atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt 120
gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa 180
aaagccctgt ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt 240
gtcaggcatg agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc 300
aatgcagttt tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc 360
acgaaaagga gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga 420
agggaattgg gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca 480
tgcgtcctta gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa 540
cactggtaac tataagacaa acattgcaga caggatagag cagatttttg agacagcccc 600
ttttgttaaa atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg 660
gagtactata ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat 720
tgagcatcta tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc 780
aggactggta tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat 840
actatatttc ttccacaaga actttgagga agagataaga agaatgtttg agccagggca 900
ggagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa 960
atctccttat tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg 1020
ctatatgggt caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga 1080
aatgtctgtt ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag 1140
aagattcttc agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac 1200
tgacgtagca ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg 1260
tgaaaccaga agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa 1320
gagatctcac atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc 1380
attcgccgag tttctaaaca agacatattc gagtgactca taacatgaaa aaaactaaca 1440
cccctcccgt acgccaccat gttcgtgttt ctggtgctgc tgcctctggt gagctcccag 1500
tgcgtgaact tcaccacaag gacccagctg ccccctgcct ataccaattc cttcacacgg 1560
ggcgtgtact atcccgacaa ggtgttccgg agcagcgtgc tgcactccac acaggatctg 1620
tttctgcctt tcttttctaa cgtgacctgg ttccacgcca tccacgtgag cggcaccaat 1680
ggcacaaagc ggttcgccaa tccagtgctg ccctttaacg atggcgtgta cttcgcctcc 1740
accgagaagt ctaacatcat cagaggctgg atctttggca ccacactgga cagcaagaca 1800
cagtccctgc tgatcgtgaa caatgccacc aacgtggtca tcaaggtgtg cgagttccag 1860
ttttgtaatg atccattcct gggcgtgtac tatcacaaga acaataagtc ttggatggag 1920
agcgagtttc gcgtgtattc ctctgccaac aattgcacat ttgagtacgt gtcccagccc 1980
ttcctgatgg acctggaggg caagcagggc aatttcaaga acctgaggga gttcgtgttt 2040
aagaatatcg atggctactt caaaatctac tccaagcaca ccccaatcaa cctggtgcgc 2100
ggcctgccac agggcttctc tgccctggag ccactggtgg atctgcccat cggcatcaac 2160
atcacccggt ttcagacact gctggccctg cacagaagct acctgacacc aggcgacagc 2220
tcctctggat ggaccgcagg agcagcagcc tactatgtgg gctatctgca gcccaggacc 2280
ttcctgctga agtacaacga gaatggcacc atcacagacg ccgtggattg cgccctggat 2340
cccctgtctg agaccaagtg tacactgaag agctttaccg tggagaaggg catctatcag 2400
acaagcaatt tcagggtgca gcctaccgag tccatcgtgc gctttcccaa tatcacaaac 2460
ctgtgccctt ttggcgaggt gttcaacgca acccgcttcg ccagcgtgta cgcctggaat 2520
aggaagcgca tctccaactg cgtggccgac tattctgtgc tgtacaacag cgcctccttc 2580
tctaccttta agtgctatgg cgtgagcccc acaaagctga atgacctgtg ctttaccaac 2640
gtgtacgccg attccttcgt gatcaggggc gacgaggtgc gccagatcgc accaggacag 2700
acaggcaata tcgcagacta caattataag ctgcctgacg atttcaccgg ctgcgtgatc 2760
gcctggaact ctaacaatct ggatagcaaa gtgggcggca actacaatta tctgtaccgg 2820
ctgtttagaa agtctaatct gaagccattc gagagggaca tctccacaga aatctaccag 2880
gccggctcta ccccctgcaa tggcgtgaag ggctttaact gttatttccc tctgcagagc 2940
tacggcttcc agccaacata tggcgtgggc tatcagccct accgcgtggt ggtgctgtct 3000
tttgagctgc tgcacgcacc tgcaacagtg tgcggaccaa agaagagcac caatctggtg 3060
aagaacaagt gcgtgaactt caacttcaac ggactgaccg gaacaggcgt gctgaccgag 3120
tccaacaaga agttcctgcc ttttcagcag ttcggcaggg acatcgcaga taccacagac 3180
gccgtgcgcg accctcagac cctggagatc ctggacatca caccatgctc cttcggcggc 3240
gtgtctgtga tcacaccagg caccaataca agcaaccagg tggccgtgct gtatcagggc 3300
gtgaattgta ccgaggtgcc agtggcaatc cacgcagatc agctgacccc tacatggcgg 3360
gtgtactcta ccggcagcaa cgtgttccag acaagagccg gatgcctgat cggagcagag 3420
cacgtgaaca atagctatga gtgcgacatc cctatcggcg ccggcatctg tgcctcctac 3480
cagacccaga caaactcccc aaggtctgtg ggcgatacag gcctgtccaa gaatccaatc 3540
gagctggtag agggctggtt cagcagttgg aaaagctcca tcgcctcctt tttctttatc 3600
atcggcctga tcatcggact gttcctggtg ctccgcgtgg gtatccacct gtgcatcaag 3660
ctgaagcaca ccaagaaaag acagatttat acagacatcg agatgaaccg cctgggaaag 3720
tgagctagcc atgaaaaaaa ctaacacccc tcctttcgaa ccatcccaaa catgagcaag 3780
atctttgtca atcctagtgc tattagagcc ggtctggccg atcttgagat ggctgaagaa 3840
actgttgatc tgatcaatag aaatatcgaa gacaatcagg ctcatctcca aggggaaccc 3900
atagaggtgg acaatctccc tgaggatatg gggcgacttc acctggatga tggaaaatcg 3960
cccaaccatg gtgagatagc caaggtggga gaaggcaagt atcgagagga ctttcagatg 4020
gatgaaggag aggatcctag cttcctgttc cagtcatacc tggaaaatgt tggagtccaa 4080
atagtcagac aaatgaggtc aggagagaga tttctcaaga tatggtcaca gaccgtagaa 4140
gagattatat cctatgtcgc ggtcaacttt cccaaccctc caggaaagtc ttcagaggat 4200
aaatcaaccc agactactgg ccgagagctc aagaaggaga caacacccac tccttctcag 4260
agagaaagcc aatcatcgaa agccaggatg gcggctcaaa ttgcttctgg ccctccagcc 4320
cttgaatggt cggctaccaa tgaagaggat gatctatcag tggaggctga gatcgctcac 4380
cagattgcag aaagtttctc caaaaaatat aagtttccct ctcgatcctc agggatactc 4440
ttgtataatt ttgagcaatt gaaaatgaac cttgatgata tagttaaaga ggcaaaaaat 4500
gtaccaggtg tgacccgttt agcccatgac gggtccaaac tccccctaag atgtgtactg 4560
ggatgggtcg ctttggccaa ctctaagaaa ttccagttgt tagtcgaatc cgacaagctg 4620
agtaaaatca tgcaagatga cttgaatcgc tatacatctt gctaaccgaa cctctcccct 4680
cagtccctct agacaataaa atccgagatg tcccaaagtc aacatgaaaa aaacaggcaa 4740
caccactgat aaaatgaacc tcctacgtaa gatagtgaaa aaccgcaggg acgaggacac 4800
tcaaaaatcc tctcccgcgt cagcccctct ggatgacgat gacttgtggc ttccaccccc 4860
tgaatacgtc ccgctgaaag aacttacagg caagaagaac atgaggaact tttgtatcaa 4920
cggaagggtt aaagtgtgta gcccgaatgg ttactcgttc aggatcctgc ggcacattct 4980
gaaatcattc gacgagatat attctgggaa tcataggatg atcgggttag tcaaagtggt 5040
tattggactg gctttgtcag gatctccagt ccctgagggc ctgaactggg tatacaaatt 5100
gaggagaacc tttatcttcc agtgggctga ttccaggggc cctcttgaag gggaggagtt 5160
ggaatactct caggagatca cttgggatga tgatactgag ttcgtcggat tgcaaataag 5220
agtgattgca aaacagtgtc atatccaggg cagagtctgg tgtatcaaca tgaacccgag 5280
agcatgtcaa ctatggtctg acatgtctct tcagacacaa aggtccgaag aggacaaaga 5340
ttcctctctg cttctagaat aatcagatta tatcccgcaa atttatcact tgtttacctc 5400
tggaggagag aacatatggg ctcaactcca acccttggga gcaatataac aaaaaacatg 5460
ttatggtgcc attaaaccgc tgcatttcat caaagtcaag ttgattacct ttacattttg 5520
atcctcttgg atgtgaaaaa aactattaac atccctcaaa agaccccggg aaagatggtt 5580
cctcaggctc tcctgtttgt accccttctg gtttttccat tgtgttttgg gaaattccct 5640
atttacacga taccagacaa gcttggtccc tggagtccga ttgacataca tcacctcagc 5700
tgcccaaaca atttggtagt ggaggacgaa ggatgcacca acctgtcagg gttctcctac 5760
atggaactta aagttggata catcttagcc ataaaagtga acgggttcac ttgcacaggc 5820
gttgtgacgg aggctgaaac ctacactaac ttcgttggtt atgtcacaac cacgttcaaa 5880
agaaagcatt tccgcccaac accagatgca tgtagagccg cgtacaactg gaagatggcc 5940
ggtgacccca gatatgaaga gtctctacac aatccgtacc ctgactaccg ctggcttcga 6000
actgtaaaaa ccaccaagga gtctctcgtt atcatatctc caagtgtggc agatttggac 6060
ccatatgaca gatcccttca ctcgagggtc ttccctagcg ggaagtgctc aggagtagcg 6120
gtgtcttcta cctactgctc cactaaccac gattacacca tttggatgcc cgagaatccg 6180
agactaggga tgtcttgtga catttttacc aatagtagag ggaagagagc atccaaaggg 6240
agtgagactt gcggctttgt agatgaaaga ggcctatata agtctttaaa aggagcatgc 6300
aaactcaagt tatgtggagt tctaggactt agacttatgg atggaacatg ggtctcgatg 6360
caaacatcaa atgaaaccaa atggtgccct cccgataagt tggtgaacct gcacgacttt 6420
cgctcagacg aaattgagca ccttgttgta gaggagttgg tcaggaagag agaggagtgt 6480
ctggatgcac tagagtccat catgacaacc aagtcagtga gtttcagacg tctcagtcat 6540
ttaagaaaac ttgtccctgg gtttggaaaa gcatatacca tattcaacaa gaccttgatg 6600
gaagccgatg ctcactacaa gtcagtcgag acttggaatg agatcctccc ttcaaaaggg 6660
tgtttaagag ttggggggag gtgtcatcct catgtgaacg gggtgttttt caatggtata 6720
atattaggac ctgacggcaa tgtcttaatc ccagagatgc aatcatccct cctccagcaa 6780
catatggagt tgttggaatc ctcggttatc ccccttgtgc accccctggc agacccgtct 6840
accgttttca aggacggtga cgaggctgag gattttgttg aagttcacct tcccgatgtg 6900
cacaatcagg tctcaggagt tgacttgggt ctcccgaact gggggaagta tgtattactg 6960
agtgcagggg ccctgactgc cttgatgttg ataattttcc tgatgacatg ttgtagaaga 7020
gtcaatcgat cagaacctac gcaacacaat ctcagaggga cagggaggga ggtgtcagtc 7080
actccccaaa gcgggaagat catatcttca tgggaatcac acaagagtgg gggtgagacc 7140
agactgtaat taattaacgt cctttcaacg atccaagtcc atgaaaaaaa ctaacacccc 7200
tcccgtacct agcttataaa gtgctgggtc atctaagctt ttcagtcgag aaaaaaacat 7260
tagatcagaa gaacaactgg caacacttct caacctgaga cttacttcaa gatgctcgat 7320
cctggagagg tctatgatga ccctattgac ccaatcgagt tagaggctga acccagagga 7380
acccccattg tccccaacat cttgaggaac tctgactaca atctcaactc tcctttgata 7440
gaagatcctg ctagactaat gttagaatgg ttaaaaacag ggaatagacc ttatcggatg 7500
actctaacag acaattgctc caggtctttc agagttttga aagattattt caagaaggta 7560
gatttgggtt ctctcaaggt gggcggaatg gctgcacagt caatgatttc tctctggtta 7620
tatggtgccc actctgaatc caacaggagc cggagatgta taacagactt ggcccatttc 7680
tattccaagt cgtcccccat agagaagctg ttgaatctca cgctaggaaa tagagggctg 7740
agaatccccc cagagggagt gttaagttgc cttgagaggg ttgattatga taatgcattt 7800
ggaaggtatc ttgccaacac gtattcctct tacttgttct tccatgtaat caccttatac 7860
atgaacgccc tagactggga tgaagaaaag accatcctag cattatggaa agatttaacc 7920
tcagtggaca tcgggaagga cttggtaaag ttcaaagacc aaatatgggg actgctgatc 7980
gtgacaaagg actttgttta ctcccaaagt tccaattgtc tttttgacag aaactacaca 8040
cttatgctaa aagatctttt cttgtctcgc ttcaactcct taatggtctt gctctctccc 8100
ccagagcccc gatactcaga tgacttgata tctcaactat gccagctgta cattgctggg 8160
gatcaagtct tgtctatgtg tggaaactcc ggctatgaag tcatcaaaat attggagcca 8220
tatgtcgtga atagtttagt ccagagagca gaaaagttta ggcctctcat tcattccttg 8280
ggagactttc ctgtatttat aaaagacaag gtaagtcaac ttgaagagac gttcggtccc 8340
tgtgcaagaa ggttctttag ggctctggat caattcgaca acatacatga cttggttttt 8400
gtgtttggct gttacaggca ttgggggcac ccatatatag attatcgaaa gggtctgtca 8460
aaactatatg atcaggttca ccttaaaaaa atgatagata agtcctacca ggagtgctta 8520
gcaagcgacc tagccaggag gatccttaga tggggttttg ataagtactc caagtggtat 8580
ctggattcaa gattcctagc ccgagaccac cccttgactc cttatatcaa aacccaaaca 8640
tggccaccca aacatattgt agacttggtg ggggatacat ggcacaagct cccgatcacg 8700
cagatctttg agattcctga atcaatggat ccgtcagaaa tattggatga caaatcacat 8760
tctttcacca gaacgagact agcttcttgg ctgtcagaaa accgaggggg gcctgttcct 8820
agcgaaaaag ttattatcac ggccctgtct aagccgcctg tcaatccccg agagtttctg 8880
aggtctatag acctcggagg attgccagat gaagacttga taattggcct caagccaaag 8940
gaacgggaat tgaagattga aggtcgattc tttgctctaa tgtcatggaa tctaagattg 9000
tattttgtca tcactgaaaa actcttggcc aactacatct tgccactttt tgacgcgctg 9060
actatgacag acaacctgaa caaggtgttt aaaaagctga tcgacagggt caccgggcaa 9120
gggcttttgg actattcaag ggtcacatat gcatttcacc tggactatga aaagtggaac 9180
aaccatcaaa gattagagtc aacagaggat gtattttctg tcctagatca agtgtttgga 9240
ttgaagagag tgttttctag aacacacgag ttttttcaaa aggcctggat ctattattca 9300
gacagatcag acctcatcgg gttacgggag gatcaaatat actgcttaga tgcgtccaac 9360
ggcccaacct gttggaatgg ccaggatggc gggctagaag gcttacggca gaagggctgg 9420
agtctagtca gcttattgat gatagataga gaatctcaaa tcaggaacac aagaaccaaa 9480
atactagctc aaggagacaa ccaggtttta tgtccgacat acatgttgtc gccagggcta 9540
tctcaagagg ggctcctcta tgaattggag agaatatcaa ggaatgcact ttcgatatac 9600
agagccgtcg aggaaggggc atctaagcta gggctgatca tcaagaaaga agagaccatg 9660
tgtagttatg acttcctcat ctatggaaaa acccctttgt ttagaggtaa catattggtg 9720
cctgagtcca aaagatgggc cagagtctct tgcgtctcta atgaccaaat agtcaacctc 9780
gccaatataa tgtcgacagt gtccaccaat gcgctaacag tggcacaaca ctctcaatct 9840
ttgatcaaac cgatgaggga ttttctgctc atgtcagtac aggcagtctt tcactacctg 9900
ctatttagcc caatcttaaa gggaagagtt tacaagattc tgagcgctga aggggagagc 9960
tttctcctag ccatgtcaag gataatctat ctagatcctt ctttgggagg gatatctgga 10020
atgtccctcg gaagattcca tatacgacag ttctcagacc ctgtctctga agggttatcc 10080
ttctggagag agatctggtt aagctcccaa gagtcctgga ttcacgcgtt gtgtcaagag 10140
gctggaaacc cagatcttgg agagagaaca ctcgagagct tcactcgcct tctagaagat 10200
ccgaccacct taaatatcag aggaggggcc agtcctacca ttctactcaa ggatgcaatc 10260
agaaaggctt tatatgacga ggtggacaag gtggaaaatt cagagtttcg agaggcaatc 10320
ctgttgtcca agacccatag agataatttt atactcttct taatatctgt tgagcctctg 10380
tttcctcgat ttctcagtga gctattcagt tcgtcttttt tgggaatccc cgagtcaatc 10440
attggattga tacaaaactc ccgaacgata agaaggcagt ttagaaagag tctctcaaaa 10500
actttagaag aatccttcta caactcagag atccacggga ttagtcggat gacccagaca 10560
cctcagaggg ttgggggggt gtggccttgc tcttcagaga gggcagatct acttagggag 10620
atctcttggg gaagaaaagt ggtaggcacg acagttcctc acccttctga gatgttggga 10680
ttacttccca agtcctctat ttcttgcact tgtggagcaa caggaggagg caatcctaga 10740
gtttctgtat cagtactccc gtcctttgat cagtcatttt tttcacgagg ccccctaaag 10800
ggatacttgg gctcgtccac ctctatgtcg acccagctat tccatgcatg ggaaaaagtc 10860
actaatgttc atgtggtgaa gagagctcta tcgttaaaag aatctataaa ctggttcatt 10920
actagagatt ccaacttggc tcaagctcta attaggaaca ttatgtctct gacaggccct 10980
gatttccctc tagaggaggc ccctgtcttc aaaaggacgg ggtcagcctt gcataggttc 11040
aagtctgcca gatacagcga aggagggtat tcttctgtct gcccgaacct cctctctcat 11100
atttctgtta gtacagacac catgtctgat ttgacccaag acgggaagaa ctacgatttc 11160
atgttccagc cattgatgct ttatgcacag acatggacat cagagctggt acagagagac 11220
acaaggctaa gagactctac gtttcattgg cacctccgat gcaacaggtg tgtgagaccc 11280
attgacgacg tgaccctgga gacctctcag atcttcgagt ttccggatgt gtcgaaaaga 11340
atatccagaa tggtttctgg ggctgtgcct cacttccaga ggcttcccga tatccgtctg 11400
agaccaggag attttgaatc tctaagcggt agagaaaagt ctcaccatat cggatcagct 11460
caggggctct tatactcaat cttagtggca attcacgact caggatacaa tgatggaacc 11520
atcttccctg tcaacatata cggcaaggtt tcccctagag actatttgag agggctcgca 11580
aggggagtat tgataggatc ctcgatttgc ttcttgacaa gaatgacaaa tatcaatatt 11640
aatagacctc ttgaattggt ctcaggggta atctcatata ttctcctgag gctagataac 11700
catccctcct tgtacataat gctcagagaa ccgtctctta gaggagagat attttctatc 11760
cctcagaaaa tccccgccgc ttatccaacc actatgaaag aaggcaacag atcaatcttg 11820
tgttatctcc aacatgtgct acgctatgag cgagagataa tcacggcgtc tccagagaat 11880
gactggctat ggatcttttc agactttaga agtgccaaaa tgacgtacct atccctcatt 11940
acttaccagt ctcatcttct actccagagg gttgagagaa acctatctaa gagtatgaga 12000
gataacctgc gacaattgag ttctttgatg aggcaggtgc tgggcgggca cggagaagat 12060
accttagagt cagacgacaa cattcaacga ctgctaaaag actctttacg aaggacaaga 12120
tgggtggatc aagaggtgcg ccatgcagct agaaccatga ctggagatta cagccccaac 12180
aagaaggtgt cccgtaaggt aggatgttca gaatgggtct gctctgctca acaggttgca 12240
gtctctacct cagcaaaccc ggcccctgtc tcggagcttg acataagggc cctctctaag 12300
aggttccaga accctttgat ctcgggcttg agagtggttc agtgggcaac cggtgctcat 12360
tataagctta agcctattct agatgatctc aatgttttcc catctctctg ccttgtagtt 12420
ggggacgggt caggggggat atcaagggca gtcctcaaca tgtttccaga tgccaagctt 12480
gtgttcaaca gtcttttaga ggtgaatgac ctgatggctt ccggaacaca tccactgcct 12540
ccttcagcaa tcatgagggg aggaaatgat atcgtctcca gagtgataga tcttgactca 12600
atctgggaaa aaccgtccga cttgagaaac ttggcaacct ggaaatactt ccagtcagtc 12660
caaaagcagg tcaacatgtc ctatgacctc attatttgcg atgcagaagt tactgacatt 12720
gcatctatca accggatcac cctgttaatg tccgattttg cattgtctat agatggacca 12780
ctctatttgg tcttcaaaac ttatgggact atgctagtaa atccaaacta caaggctatt 12840
caacacctgt caagagcgtt cccctcggtc acagggttta tcacccaagt aacttcgtct 12900
ttttcatctg agctctacct ccgattctcc aaacgaggga agtttttcag agatgctgag 12960
tacttgacct cttccaccct tcgagaaatg agccttgtgt tattcaattg tagcagcccc 13020
aagagtgaga tgcagagagc tcgttccttg aactatcagg atcttgtgag aggatttcct 13080
gaagaaatca tatcaaatcc ttacaatgag atgatcataa ctctgattga cagtgatgta 13140
gaatcttttc tagtccacaa gatggttgat gatcttgagt tacagagggg aactctgtct 13200
aaagtggcta tcattatagc catcatgata gttttctcca acagagtctt caacgtttcc 13260
aaacccctaa ctgacccctc gttctatcca ccgtctgatc ccaaaatcct gaggcacttc 13320
aacatatgtt gcagtactat gatgtatcta tctactgctt taggtgacgt ccctagcttc 13380
gcaagacttc acgacctgta taacagacct ataacttatt acttcagaaa gcaagtcatt 13440
cgagggaacg tttatctatc ttggagttgg tccaacgaca cctcagtgtt caaaagggta 13500
gcctgtaatt ctagcctgag tctgtcatct cactggatca ggttgattta caagatagtg 13560
aagactacca gactcgttgg cagcatcaag gatctatcca gagaagtgga aagacacctt 13620
cataggtaca acaggtggat caccctagag gatatcagat ctagatcatc cctactagac 13680
tacagttgcc tgtgaaccgg atactcctgg aagcctgccc atgctaagac tcttgtgtga 13740
tgtatcttga aaaaaacaag atcctaaatc tgaacctttg gttgtttgat tgtttttctc 13800
atttttgttg tttatttgtt aagcgt 13826
<210> 12
<211> 13926
<212> DNA
<213> 人工序列
<220>
<223> RABV载体: Coravax V4-China (S1-RABVG-T2A-P)
<400> 12
acgcttaaca accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa 60
cacccctaca atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt 120
gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa 180
aaagccctgt ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt 240
gtcaggcatg agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc 300
aatgcagttt tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc 360
acgaaaagga gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga 420
agggaattgg gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca 480
tgcgtcctta gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa 540
cactggtaac tataagacaa acattgcaga caggatagag cagatttttg agacagcccc 600
ttttgttaaa atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg 660
gagtactata ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat 720
tgagcatcta tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc 780
aggactggta tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat 840
actatatttc ttccacaaga actttgagga agagataaga agaatgtttg agccagggca 900
ggagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa 960
atctccttat tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg 1020
ctatatgggt caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga 1080
aatgtctgtt ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag 1140
aagattcttc agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac 1200
tgacgtagca ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg 1260
tgaaaccaga agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa 1320
gagatctcac atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc 1380
attcgccgag tttctaaaca agacatattc gagtgactca taacatgaaa aaaactaaca 1440
cccctcccgt acggccacca tgttcgtgtt tctggtgctg ctgcctctgg tgagctccca 1500
gtgcgtgaac ctgaccacaa ggacccagct gccccctgcc tataccaatt ccttcacacg 1560
gggcgtgtac tatcccgaca aggtgttccg gagcagcgtg ctgcactcca cacaggatct 1620
gtttctgcct ttcttttcta acgtgacctg gttccacgcc atccacgtga gcggcaccaa 1680
tggcacaaag cggttcgaca atccagtgct gccctttaac gatggcgtgt acttcgcctc 1740
caccgagaag tctaacatca tcagaggctg gatctttggc accacactgg acagcaagac 1800
acagtccctg ctgatcgtga acaatgccac caacgtggtc atcaaggtgt gcgagttcca 1860
gttttgtaat gatccattcc tgggcgtgta ctatcacaag aacaataagt cttggatgga 1920
gagcgagttt cgcgtgtatt cctctgccaa caattgcaca tttgagtacg tgtcccagcc 1980
cttcctgatg gacctggagg gcaagcaggg caatttcaag aacctgaggg agttcgtgtt 2040
taagaatatc gatggctact tcaaaatcta ctccaagcac accccaatca acctggtgcg 2100
cgacctgcca cagggcttct ctgccctgga gccactggtg gatctgccca tcggcatcaa 2160
catcacccgg tttcagacac tgctggccct gcacagaagc tacctgacac caggcgacag 2220
ctcctctgga tggaccgcag gagcagcagc ctactatgtg ggctatctgc agcccaggac 2280
cttcctgctg aagtacaacg agaatggcac catcacagac gccgtggatt gcgccctgga 2340
tcccctgtct gagaccaagt gtacactgaa gagctttacc gtggagaagg gcatctatca 2400
gacaagcaat ttcagggtgc agcctaccga gtccatcgtg cgctttccca atatcacaaa 2460
cctgtgccct tttggcgagg tgttcaacgc aacccgcttc gccagcgtgt acgcctggaa 2520
taggaagcgc atctccaact gcgtggccga ctattctgtg ctgtacaaca gcgcctcctt 2580
ctctaccttt aagtgctatg gcgtgagccc cacaaagctg aatgacctgt gctttaccaa 2640
cgtgtacgcc gattccttcg tgatcagggg cgacgaggtg cgccagatcg caccaggaca 2700
gacaggcaag atcgcagact acaattataa gctgcctgac gatttcaccg gctgcgtgat 2760
cgcctggaac tctaacaatc tggatagcaa agtgggcggc aactacaatt atctgtaccg 2820
gctgtttaga aagtctaatc tgaagccatt cgagagggac atctccacag aaatctacca 2880
ggccggctct accccctgca atggcgtgga gggctttaac tgttatttcc ctctgcagag 2940
ctacggcttc cagccaacaa acggcgtggg ctatcagccc taccgcgtgg tggtgctgtc 3000
ttttgagctg ctgcacgcac ctgcaacagt gtgcggacca aagaagagca ccaatctggt 3060
gaagaacaag tgcgtgaact tcaacttcaa cggactgacc ggaacaggcg tgctgaccga 3120
gtccaacaag aagttcctgc cttttcagca gttcggcagg gacatcgcag ataccacaga 3180
cgccgtgcgc gaccctcaga ccctggagat cctggacatc acaccatgct ccttcggcgg 3240
cgtgtctgtg atcacaccag gcaccaatac aagcaaccag gtggccgtgc tgtatcagga 3300
cgtgaattgt accgaggtgc cagtggcaat ccacgcagat cagctgaccc ctacatggcg 3360
ggtgtactct accggcagca acgtgttcca gacaagagcc ggatgcctga tcggagcaga 3420
gcacgtgaac aatagctatg agtgcgacat ccctatcggc gccggcatct gtgcctccta 3480
ccagacccag acaaactccc caaggtctgt gggagatgag gccgaagact ttgtggaagt 3540
ccacctgcct gatgtgcata accaggtgtc tggcgtcgac ctgggactgc caaattgggg 3600
caagtacgtg ctgctgagtg ctggagcact gactgccctg atgctgatca ttttcctgat 3660
gacctgctgt cggcgcgtga acagaagtga gcccactcag cacaatctgc gaggaaccgg 3720
gagagaagtg tcagtcacac ctcagagcgg gaaaatcatt agtagttggg aatcacataa 3780
aagcgggggc gagaccaggc tgggatccgg ctccggcgag ggcaggggaa gtctactaac 3840
atgcggggac gtggaggaaa atcccggccc catgagcaag atctttgtca atcctagtgc 3900
tattagagcc ggtctggccg atcttgagat ggctgaagaa actgttgatc tgatcaatag 3960
aaatatcgaa gacaatcagg ctcatctcca aggggaaccc atagaggtgg acaatctccc 4020
tgaggatatg gggcgacttc acctggatga tggaaaatcg cccaaccatg gtgagatagc 4080
caaggtggga gaaggcaagt atcgagagga ctttcagatg gatgaaggag aggatcctag 4140
cttcctgttc cagtcatacc tggaaaatgt tggagtccaa atagtcagac aaatgaggtc 4200
aggagagaga tttctcaaga tatggtcaca gaccgtagaa gagattatat cctatgtcgc 4260
ggtcaacttt cccaaccctc caggaaagtc ttcagaggat aaatcaaccc agactactgg 4320
ccgagagctc aagaaggaga caacacccac tccttctcag agagaaagcc aatcatcgaa 4380
agccaggatg gcggctcaaa ttgcttctgg ccctccagcc cttgaatggt cggctaccaa 4440
tgaagaggat gatctatcag tggaggctga gatcgctcac cagattgcag aaagtttctc 4500
caaaaaatat aagtttccct ctcgatcctc agggatactc ttgtataatt ttgagcaatt 4560
gaaaatgaac cttgatgata tagttaaaga ggcaaaaaat gtaccaggtg tgacccgttt 4620
agcccatgac gggtccaaac tccccctaag atgtgtactg ggatgggtcg ctttggccaa 4680
ctctaagaaa ttccagttgt tagtcgaatc cgacaagctg agtaaaatca tgcaagatga 4740
cttgaatcgc tatacatctt gctaaccgaa cctctcccct cagtccctct agacaataaa 4800
atccgagatg tcccaaagtc aacatgaaaa aaacaggcaa caccactgat aaaatgaacc 4860
tcctacgtaa gatagtgaaa aaccgcaggg acgaggacac tcaaaaatcc tctcccgcgt 4920
cagcccctct ggatgacgat gacttgtggc ttccaccccc tgaatacgtc ccgctgaaag 4980
aacttacagg caagaagaac atgaggaact tttgtatcaa cggaagggtt aaagtgtgta 5040
gcccgaatgg ttactcgttc aggatcctgc ggcacattct gaaatcattc gacgagatat 5100
attctgggaa tcataggatg atcgggttag tcaaagtggt tattggactg gctttgtcag 5160
gatctccagt ccctgagggc ctgaactggg tatacaaatt gaggagaacc tttatcttcc 5220
agtgggctga ttccaggggc cctcttgaag gggaggagtt ggaatactct caggagatca 5280
cttgggatga tgatactgag ttcgtcggat tgcaaataag agtgattgca aaacagtgtc 5340
atatccaggg cagagtctgg tgtatcaaca tgaacccgag agcatgtcaa ctatggtctg 5400
acatgtctct tcagacacaa aggtccgaag aggacaaaga ttcctctctg cttctagaat 5460
aatcagatta tatcccgcaa atttatcact tgtttacctc tggaggagag aacatatggg 5520
ctcaactcca acccttggga gcaatataac aaaaaacatg ttatggtgcc attaaaccgc 5580
tgcatttcat caaagtcaag ttgattacct ttacattttg atcctcttgg atgtgaaaaa 5640
aactattaac atccctcaaa agaccccggg aaagatggtt cctcaggctc tcctgtttgt 5700
accccttctg gtttttccat tgtgttttgg gaaattccct atttacacga taccagacaa 5760
gcttggtccc tggagtccga ttgacataca tcacctcagc tgcccaaaca atttggtagt 5820
ggaggacgaa ggatgcacca acctgtcagg gttctcctac atggaactta aagttggata 5880
catcttagcc ataaaagtga acgggttcac ttgcacaggc gttgtgacgg aggctgaaac 5940
ctacactaac ttcgttggtt atgtcacaac cacgttcaaa agaaagcatt tccgcccaac 6000
accagatgca tgtagagccg cgtacaactg gaagatggcc ggtgacccca gatatgaaga 6060
gtctctacac aatccgtacc ctgactaccg ctggcttcga actgtaaaaa ccaccaagga 6120
gtctctcgtt atcatatctc caagtgtggc agatttggac ccatatgaca gatcccttca 6180
ctcgagggtc ttccctagcg ggaagtgctc aggagtagcg gtgtcttcta cctactgctc 6240
cactaaccac gattacacca tttggatgcc cgagaatccg agactaggga tgtcttgtga 6300
catttttacc aatagtagag ggaagagagc atccaaaggg agtgagactt gcggctttgt 6360
agatgaaaga ggcctatata agtctttaaa aggagcatgc aaactcaagt tatgtggagt 6420
tctaggactt agacttatgg atggaacatg ggtctcgatg caaacatcaa atgaaaccaa 6480
atggtgccct cccgataagt tggtgaacct gcacgacttt cgctcagacg aaattgagca 6540
ccttgttgta gaggagttgg tcaggaagag agaggagtgt ctggatgcac tagagtccat 6600
catgacaacc aagtcagtga gtttcagacg tctcagtcat ttaagaaaac ttgtccctgg 6660
gtttggaaaa gcatatacca tattcaacaa gaccttgatg gaagccgatg ctcactacaa 6720
gtcagtcgag acttggaatg agatcctccc ttcaaaaggg tgtttaagag ttggggggag 6780
gtgtcatcct catgtgaacg gggtgttttt caatggtata atattaggac ctgacggcaa 6840
tgtcttaatc ccagagatgc aatcatccct cctccagcaa catatggagt tgttggaatc 6900
ctcggttatc ccccttgtgc accccctggc agacccgtct accgttttca aggacggtga 6960
cgaggctgag gattttgttg aagttcacct tcccgatgtg cacaatcagg tctcaggagt 7020
tgacttgggt ctcccgaact gggggaagta tgtattactg agtgcagggg ccctgactgc 7080
cttgatgttg ataattttcc tgatgacatg ttgtagaaga gtcaatcgat cagaacctac 7140
gcaacacaat ctcagaggga cagggaggga ggtgtcagtc actccccaaa gcgggaagat 7200
catatcttca tgggaatcac acaagagtgg gggtgagacc agactgtaat taattaacgt 7260
cctttcaacg atccaagtcc atgaaaaaaa ctaacacccc tcccgtacct agcttataaa 7320
gtgctgggtc atctaagctt ttcagtcgag aaaaaaacat tagatcagaa gaacaactgg 7380
caacacttct caacctgaga cttacttcaa gatgctcgat cctggagagg tctatgatga 7440
ccctattgac ccaatcgagt tagaggctga acccagagga acccccattg tccccaacat 7500
cttgaggaac tctgactaca atctcaactc tcctttgata gaagatcctg ctagactaat 7560
gttagaatgg ttaaaaacag ggaatagacc ttatcggatg actctaacag acaattgctc 7620
caggtctttc agagttttga aagattattt caagaaggta gatttgggtt ctctcaaggt 7680
gggcggaatg gctgcacagt caatgatttc tctctggtta tatggtgccc actctgaatc 7740
caacaggagc cggagatgta taacagactt ggcccatttc tattccaagt cgtcccccat 7800
agagaagctg ttgaatctca cgctaggaaa tagagggctg agaatccccc cagagggagt 7860
gttaagttgc cttgagaggg ttgattatga taatgcattt ggaaggtatc ttgccaacac 7920
gtattcctct tacttgttct tccatgtaat caccttatac atgaacgccc tagactggga 7980
tgaagaaaag accatcctag cattatggaa agatttaacc tcagtggaca tcgggaagga 8040
cttggtaaag ttcaaagacc aaatatgggg actgctgatc gtgacaaagg actttgttta 8100
ctcccaaagt tccaattgtc tttttgacag aaactacaca cttatgctaa aagatctttt 8160
cttgtctcgc ttcaactcct taatggtctt gctctctccc ccagagcccc gatactcaga 8220
tgacttgata tctcaactat gccagctgta cattgctggg gatcaagtct tgtctatgtg 8280
tggaaactcc ggctatgaag tcatcaaaat attggagcca tatgtcgtga atagtttagt 8340
ccagagagca gaaaagttta ggcctctcat tcattccttg ggagactttc ctgtatttat 8400
aaaagacaag gtaagtcaac ttgaagagac gttcggtccc tgtgcaagaa ggttctttag 8460
ggctctggat caattcgaca acatacatga cttggttttt gtgtttggct gttacaggca 8520
ttgggggcac ccatatatag attatcgaaa gggtctgtca aaactatatg atcaggttca 8580
ccttaaaaaa atgatagata agtcctacca ggagtgctta gcaagcgacc tagccaggag 8640
gatccttaga tggggttttg ataagtactc caagtggtat ctggattcaa gattcctagc 8700
ccgagaccac cccttgactc cttatatcaa aacccaaaca tggccaccca aacatattgt 8760
agacttggtg ggggatacat ggcacaagct cccgatcacg cagatctttg agattcctga 8820
atcaatggat ccgtcagaaa tattggatga caaatcacat tctttcacca gaacgagact 8880
agcttcttgg ctgtcagaaa accgaggggg gcctgttcct agcgaaaaag ttattatcac 8940
ggccctgtct aagccgcctg tcaatccccg agagtttctg aggtctatag acctcggagg 9000
attgccagat gaagacttga taattggcct caagccaaag gaacgggaat tgaagattga 9060
aggtcgattc tttgctctaa tgtcatggaa tctaagattg tattttgtca tcactgaaaa 9120
actcttggcc aactacatct tgccactttt tgacgcgctg actatgacag acaacctgaa 9180
caaggtgttt aaaaagctga tcgacagggt caccgggcaa gggcttttgg actattcaag 9240
ggtcacatat gcatttcacc tggactatga aaagtggaac aaccatcaaa gattagagtc 9300
aacagaggat gtattttctg tcctagatca agtgtttgga ttgaagagag tgttttctag 9360
aacacacgag ttttttcaaa aggcctggat ctattattca gacagatcag acctcatcgg 9420
gttacgggag gatcaaatat actgcttaga tgcgtccaac ggcccaacct gttggaatgg 9480
ccaggatggc gggctagaag gcttacggca gaagggctgg agtctagtca gcttattgat 9540
gatagataga gaatctcaaa tcaggaacac aagaaccaaa atactagctc aaggagacaa 9600
ccaggtttta tgtccgacat acatgttgtc gccagggcta tctcaagagg ggctcctcta 9660
tgaattggag agaatatcaa ggaatgcact ttcgatatac agagccgtcg aggaaggggc 9720
atctaagcta gggctgatca tcaagaaaga agagaccatg tgtagttatg acttcctcat 9780
ctatggaaaa acccctttgt ttagaggtaa catattggtg cctgagtcca aaagatgggc 9840
cagagtctct tgcgtctcta atgaccaaat agtcaacctc gccaatataa tgtcgacagt 9900
gtccaccaat gcgctaacag tggcacaaca ctctcaatct ttgatcaaac cgatgaggga 9960
ttttctgctc atgtcagtac aggcagtctt tcactacctg ctatttagcc caatcttaaa 10020
gggaagagtt tacaagattc tgagcgctga aggggagagc tttctcctag ccatgtcaag 10080
gataatctat ctagatcctt ctttgggagg gatatctgga atgtccctcg gaagattcca 10140
tatacgacag ttctcagacc ctgtctctga agggttatcc ttctggagag agatctggtt 10200
aagctcccaa gagtcctgga ttcacgcgtt gtgtcaagag gctggaaacc cagatcttgg 10260
agagagaaca ctcgagagct tcactcgcct tctagaagat ccgaccacct taaatatcag 10320
aggaggggcc agtcctacca ttctactcaa ggatgcaatc agaaaggctt tatatgacga 10380
ggtggacaag gtggaaaatt cagagtttcg agaggcaatc ctgttgtcca agacccatag 10440
agataatttt atactcttct taatatctgt tgagcctctg tttcctcgat ttctcagtga 10500
gctattcagt tcgtcttttt tgggaatccc cgagtcaatc attggattga tacaaaactc 10560
ccgaacgata agaaggcagt ttagaaagag tctctcaaaa actttagaag aatccttcta 10620
caactcagag atccacggga ttagtcggat gacccagaca cctcagaggg ttgggggggt 10680
gtggccttgc tcttcagaga gggcagatct acttagggag atctcttggg gaagaaaagt 10740
ggtaggcacg acagttcctc acccttctga gatgttggga ttacttccca agtcctctat 10800
ttcttgcact tgtggagcaa caggaggagg caatcctaga gtttctgtat cagtactccc 10860
gtcctttgat cagtcatttt tttcacgagg ccccctaaag ggatacttgg gctcgtccac 10920
ctctatgtcg acccagctat tccatgcatg ggaaaaagtc actaatgttc atgtggtgaa 10980
gagagctcta tcgttaaaag aatctataaa ctggttcatt actagagatt ccaacttggc 11040
tcaagctcta attaggaaca ttatgtctct gacaggccct gatttccctc tagaggaggc 11100
ccctgtcttc aaaaggacgg ggtcagcctt gcataggttc aagtctgcca gatacagcga 11160
aggagggtat tcttctgtct gcccgaacct cctctctcat atttctgtta gtacagacac 11220
catgtctgat ttgacccaag acgggaagaa ctacgatttc atgttccagc cattgatgct 11280
ttatgcacag acatggacat cagagctggt acagagagac acaaggctaa gagactctac 11340
gtttcattgg cacctccgat gcaacaggtg tgtgagaccc attgacgacg tgaccctgga 11400
gacctctcag atcttcgagt ttccggatgt gtcgaaaaga atatccagaa tggtttctgg 11460
ggctgtgcct cacttccaga ggcttcccga tatccgtctg agaccaggag attttgaatc 11520
tctaagcggt agagaaaagt ctcaccatat cggatcagct caggggctct tatactcaat 11580
cttagtggca attcacgact caggatacaa tgatggaacc atcttccctg tcaacatata 11640
cggcaaggtt tcccctagag actatttgag agggctcgca aggggagtat tgataggatc 11700
ctcgatttgc ttcttgacaa gaatgacaaa tatcaatatt aatagacctc ttgaattggt 11760
ctcaggggta atctcatata ttctcctgag gctagataac catccctcct tgtacataat 11820
gctcagagaa ccgtctctta gaggagagat attttctatc cctcagaaaa tccccgccgc 11880
ttatccaacc actatgaaag aaggcaacag atcaatcttg tgttatctcc aacatgtgct 11940
acgctatgag cgagagataa tcacggcgtc tccagagaat gactggctat ggatcttttc 12000
agactttaga agtgccaaaa tgacgtacct atccctcatt acttaccagt ctcatcttct 12060
actccagagg gttgagagaa acctatctaa gagtatgaga gataacctgc gacaattgag 12120
ttctttgatg aggcaggtgc tgggcgggca cggagaagat accttagagt cagacgacaa 12180
cattcaacga ctgctaaaag actctttacg aaggacaaga tgggtggatc aagaggtgcg 12240
ccatgcagct agaaccatga ctggagatta cagccccaac aagaaggtgt cccgtaaggt 12300
aggatgttca gaatgggtct gctctgctca acaggttgca gtctctacct cagcaaaccc 12360
ggcccctgtc tcggagcttg acataagggc cctctctaag aggttccaga accctttgat 12420
ctcgggcttg agagtggttc agtgggcaac cggtgctcat tataagctta agcctattct 12480
agatgatctc aatgttttcc catctctctg ccttgtagtt ggggacgggt caggggggat 12540
atcaagggca gtcctcaaca tgtttccaga tgccaagctt gtgttcaaca gtcttttaga 12600
ggtgaatgac ctgatggctt ccggaacaca tccactgcct ccttcagcaa tcatgagggg 12660
aggaaatgat atcgtctcca gagtgataga tcttgactca atctgggaaa aaccgtccga 12720
cttgagaaac ttggcaacct ggaaatactt ccagtcagtc caaaagcagg tcaacatgtc 12780
ctatgacctc attatttgcg atgcagaagt tactgacatt gcatctatca accggatcac 12840
cctgttaatg tccgattttg cattgtctat agatggacca ctctatttgg tcttcaaaac 12900
ttatgggact atgctagtaa atccaaacta caaggctatt caacacctgt caagagcgtt 12960
cccctcggtc acagggttta tcacccaagt aacttcgtct ttttcatctg agctctacct 13020
ccgattctcc aaacgaggga agtttttcag agatgctgag tacttgacct cttccaccct 13080
tcgagaaatg agccttgtgt tattcaattg tagcagcccc aagagtgaga tgcagagagc 13140
tcgttccttg aactatcagg atcttgtgag aggatttcct gaagaaatca tatcaaatcc 13200
ttacaatgag atgatcataa ctctgattga cagtgatgta gaatcttttc tagtccacaa 13260
gatggttgat gatcttgagt tacagagggg aactctgtct aaagtggcta tcattatagc 13320
catcatgata gttttctcca acagagtctt caacgtttcc aaacccctaa ctgacccctc 13380
gttctatcca ccgtctgatc ccaaaatcct gaggcacttc aacatatgtt gcagtactat 13440
gatgtatcta tctactgctt taggtgacgt ccctagcttc gcaagacttc acgacctgta 13500
taacagacct ataacttatt acttcagaaa gcaagtcatt cgagggaacg tttatctatc 13560
ttggagttgg tccaacgaca cctcagtgtt caaaagggta gcctgtaatt ctagcctgag 13620
tctgtcatct cactggatca ggttgattta caagatagtg aagactacca gactcgttgg 13680
cagcatcaag gatctatcca gagaagtgga aagacacctt cataggtaca acaggtggat 13740
caccctagag gatatcagat ctagatcatc cctactagac tacagttgcc tgtgaaccgg 13800
atactcctgg aagcctgccc atgctaagac tcttgtgtga tgtatcttga aaaaaacaag 13860
atcctaaatc tgaacctttg gttgtttgat tgtttttctc atttttgttg tttatttgtt 13920
aagcgt 13926
<210> 13
<211> 13977
<212> DNA
<213> 人工序列
<220>
<223> RABV载体: Coravax V4 South Africa
<400> 13
acgcttaaca accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa 60
cacccctaca atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt 120
gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa 180
aaagccctgt ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt 240
gtcaggcatg agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc 300
aatgcagttt tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc 360
acgaaaagga gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga 420
agggaattgg gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca 480
tgcgtcctta gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa 540
cactggtaac tataagacaa acattgcaga caggatagag cagatttttg agacagcccc 600
ttttgttaaa atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg 660
gagtactata ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat 720
tgagcatcta tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc 780
aggactggta tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat 840
actatatttc ttccacaaga actttgagga agagataaga agaatgtttg agccagggca 900
ggagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa 960
atctccttat tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg 1020
ctatatgggt caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga 1080
aatgtctgtt ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag 1140
aagattcttc agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac 1200
tgacgtagca ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg 1260
tgaaaccaga agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa 1320
gagatctcac atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc 1380
attcgccgag tttctaaaca agacatattc gagtgactca taacatgaaa aaaactaaca 1440
cccctcccgt acggccacca tgttcgtgtt tctggtgctg ctgcctctgg tgagctccca 1500
gtgcgtgaac ttcaccacaa ggacccagct gccccctgcc tataccaatt ccttcacacg 1560
gggcgtgtac tatcccgaca aggtgttccg gagcagcgtg ctgcactcca cacaggatct 1620
gtttctgcct ttcttttcta acgtgacctg gttccacgcc atccacgtga gcggcaccaa 1680
tggcacaaag cggttcgcca atccagtgct gccctttaac gatggcgtgt acttcgcctc 1740
caccgagaag tctaacatca tcagaggctg gatctttggc accacactgg acagcaagac 1800
acagtccctg ctgatcgtga acaatgccac caacgtggtc atcaaggtgt gcgagttcca 1860
gttttgtaat gatccattcc tgggcgtgta ctatcacaag aacaataagt cttggatgga 1920
gagcgagttt cgcgtgtatt cctctgccaa caattgcaca tttgagtacg tgtcccagcc 1980
cttcctgatg gacctggagg gcaagcaggg caatttcaag aacctgaggg agttcgtgtt 2040
taagaatatc gatggctact tcaaaatcta ctccaagcac accccaatca acctggtgcg 2100
cggcctgcca cagggcttct ctgccctgga gccactggtg gatctgccca tcggcatcaa 2160
catcacccgg tttcagacac tgctggccct gcacagaagc tacctgacac caggcgacag 2220
ctcctctgga tggaccgcag gagcagcagc ctactatgtg ggctatctgc agcccaggac 2280
cttcctgctg aagtacaacg agaatggcac catcacagac gccgtggatt gcgccctgga 2340
tcccctgtct gagaccaagt gtacactgaa gagctttacc gtggagaagg gcatctatca 2400
gacaagcaat ttcagggtgc agcctaccga gtccatcgtg cgctttccca atatcacaaa 2460
cctgtgccct tttggcgagg tgttcaacgc aacccgcttc gccagcgtgt acgcctggaa 2520
taggaagcgc atctccaact gcgtggccga ctattctgtg ctgtacaaca gcgcctcctt 2580
ctctaccttt aagtgctatg gcgtgagccc cacaaagctg aatgacctgt gctttaccaa 2640
cgtgtacgcc gattccttcg tgatcagggg cgacgaggtg cgccagatcg caccaggaca 2700
gacaggcaat atcgcagact acaattataa gctgcctgac gatttcaccg gctgcgtgat 2760
cgcctggaac tctaacaatc tggatagcaa agtgggcggc aactacaatt atctgtaccg 2820
gctgtttaga aagtctaatc tgaagccatt cgagagggac atctccacag aaatctacca 2880
ggccggctct accccctgca atggcgtgaa gggctttaac tgttatttcc ctctgcagag 2940
ctacggcttc cagccaacat atggcgtggg ctatcagccc taccgcgtgg tggtgctgtc 3000
ttttgagctg ctgcacgcac ctgcaacagt gtgcggacca aagaagagca ccaatctggt 3060
gaagaacaag tgcgtgaact tcaacttcaa cggactgacc ggaacaggcg tgctgaccga 3120
gtccaacaag aagttcctgc cttttcagca gttcggcagg gacatcgcag ataccacaga 3180
cgccgtgcgc gaccctcaga ccctggagat cctggacatc acaccatgct ccttcggcgg 3240
cgtgtctgtg atcacaccag gcaccaatac aagcaaccag gtggccgtgc tgtatcaggg 3300
cgtgaattgt accgaggtgc cagtggcaat ccacgcagat cagctgaccc ctacatggcg 3360
ggtgtactct accggcagca acgtgttcca gacaagagcc ggatgcctga tcggagcaga 3420
gcacgtgaac aatagctatg agtgcgacat ccctatcggc gccggcatct gtgcctccta 3480
ccagacccag acaaactccc cagaatcaag cgtgattcct ctggtccatc cactggcaga 3540
tccctccaca gtgttcaaag acggagatga ggccgaagac tttgtggaag tccacctgcc 3600
tgatgtgcat aaccaggtgt ctggcgtcga cctgggactg ccaaattggg gcaagtacgt 3660
gctgctgagt gctggagcac tgactgccct gatgctgatc attttcctga tgacctgctg 3720
tcggcgcgtg aacagaagtg agcccactca gcacaatctg cgaggaaccg ggagagaagt 3780
gtcagtcaca cctcagagcg ggaaaatcat tagtagttgg gaatcacata aaagcggggg 3840
cgagaccagg ctgggatccg gctccggcga gggcagggga agtctactaa catgcgggga 3900
cgtggaggaa aatcccggcc ccatgagcaa gatctttgtc aatcctagtg ctattagagc 3960
cggtctggcc gatcttgaga tggctgaaga aactgttgat ctgatcaata gaaatatcga 4020
agacaatcag gctcatctcc aaggggaacc catagaggtg gacaatctcc ctgaggatat 4080
ggggcgactt cacctggatg atggaaaatc gcccaaccat ggtgagatag ccaaggtggg 4140
agaaggcaag tatcgagagg actttcagat ggatgaagga gaggatccta gcttcctgtt 4200
ccagtcatac ctggaaaatg ttggagtcca aatagtcaga caaatgaggt caggagagag 4260
atttctcaag atatggtcac agaccgtaga agagattata tcctatgtcg cggtcaactt 4320
tcccaaccct ccaggaaagt cttcagagga taaatcaacc cagactactg gccgagagct 4380
caagaaggag acaacaccca ctccttctca gagagaaagc caatcatcga aagccaggat 4440
ggcggctcaa attgcttctg gccctccagc ccttgaatgg tcggctacca atgaagagga 4500
tgatctatca gtggaggctg agatcgctca ccagattgca gaaagtttct ccaaaaaata 4560
taagtttccc tctcgatcct cagggatact cttgtataat tttgagcaat tgaaaatgaa 4620
ccttgatgat atagttaaag aggcaaaaaa tgtaccaggt gtgacccgtt tagcccatga 4680
cgggtccaaa ctccccctaa gatgtgtact gggatgggtc gctttggcca actctaagaa 4740
attccagttg ttagtcgaat ccgacaagct gagtaaaatc atgcaagatg acttgaatcg 4800
ctatacatct tgctaaccga acctctcccc tcagtccctc tagacaataa aatccgagat 4860
gtcccaaagt caacatgaaa aaaacaggca acaccactga taaaatgaac ctcctacgta 4920
agatagtgaa aaaccgcagg gacgaggaca ctcaaaaatc ctctcccgcg tcagcccctc 4980
tggatgacga tgacttgtgg cttccacccc ctgaatacgt cccgctgaaa gaacttacag 5040
gcaagaagaa catgaggaac ttttgtatca acggaagggt taaagtgtgt agcccgaatg 5100
gttactcgtt caggatcctg cggcacattc tgaaatcatt cgacgagata tattctggga 5160
atcataggat gatcgggtta gtcaaagtgg ttattggact ggctttgtca ggatctccag 5220
tccctgaggg cctgaactgg gtatacaaat tgaggagaac ctttatcttc cagtgggctg 5280
attccagggg ccctcttgaa ggggaggagt tggaatactc tcaggagatc acttgggatg 5340
atgatactga gttcgtcgga ttgcaaataa gagtgattgc aaaacagtgt catatccagg 5400
gcagagtctg gtgtatcaac atgaacccga gagcatgtca actatggtct gacatgtctc 5460
ttcagacaca aaggtccgaa gaggacaaag attcctctct gcttctagaa taatcagatt 5520
atatcccgca aatttatcac ttgtttacct ctggaggaga gaacatatgg gctcaactcc 5580
aacccttggg agcaatataa caaaaaacat gttatggtgc cattaaaccg ctgcatttca 5640
tcaaagtcaa gttgattacc tttacatttt gatcctcttg gatgtgaaaa aaactattaa 5700
catccctcaa aagaccccgg gaaagatggt tcctcaggct ctcctgtttg taccccttct 5760
ggtttttcca ttgtgttttg ggaaattccc tatttacacg ataccagaca agcttggtcc 5820
ctggagtccg attgacatac atcacctcag ctgcccaaac aatttggtag tggaggacga 5880
aggatgcacc aacctgtcag ggttctccta catggaactt aaagttggat acatcttagc 5940
cataaaagtg aacgggttca cttgcacagg cgttgtgacg gaggctgaaa cctacactaa 6000
cttcgttggt tatgtcacaa ccacgttcaa aagaaagcat ttccgcccaa caccagatgc 6060
atgtagagcc gcgtacaact ggaagatggc cggtgacccc agatatgaag agtctctaca 6120
caatccgtac cctgactacc gctggcttcg aactgtaaaa accaccaagg agtctctcgt 6180
tatcatatct ccaagtgtgg cagatttgga cccatatgac agatcccttc actcgagggt 6240
cttccctagc gggaagtgct caggagtagc ggtgtcttct acctactgct ccactaacca 6300
cgattacacc atttggatgc ccgagaatcc gagactaggg atgtcttgtg acatttttac 6360
caatagtaga gggaagagag catccaaagg gagtgagact tgcggctttg tagatgaaag 6420
aggcctatat aagtctttaa aaggagcatg caaactcaag ttatgtggag ttctaggact 6480
tagacttatg gatggaacat gggtctcgat gcaaacatca aatgaaacca aatggtgccc 6540
tcccgataag ttggtgaacc tgcacgactt tcgctcagac gaaattgagc accttgttgt 6600
agaggagttg gtcaggaaga gagaggagtg tctggatgca ctagagtcca tcatgacaac 6660
caagtcagtg agtttcagac gtctcagtca tttaagaaaa cttgtccctg ggtttggaaa 6720
agcatatacc atattcaaca agaccttgat ggaagccgat gctcactaca agtcagtcga 6780
gacttggaat gagatcctcc cttcaaaagg gtgtttaaga gttgggggga ggtgtcatcc 6840
tcatgtgaac ggggtgtttt tcaatggtat aatattagga cctgacggca atgtcttaat 6900
cccagagatg caatcatccc tcctccagca acatatggag ttgttggaat cctcggttat 6960
cccccttgtg caccccctgg cagacccgtc taccgttttc aaggacggtg acgaggctga 7020
ggattttgtt gaagttcacc ttcccgatgt gcacaatcag gtctcaggag ttgacttggg 7080
tctcccgaac tgggggaagt atgtattact gagtgcaggg gccctgactg ccttgatgtt 7140
gataattttc ctgatgacat gttgtagaag agtcaatcga tcagaaccta cgcaacacaa 7200
tctcagaggg acagggaggg aggtgtcagt cactccccaa agcgggaaga tcatatcttc 7260
atgggaatca cacaagagtg ggggtgagac cagactgtaa ttaattaacg tcctttcaac 7320
gatccaagtc catgaaaaaa actaacaccc ctcccgtacc tagcttataa agtgctgggt 7380
catctaagct tttcagtcga gaaaaaaaca ttagatcaga agaacaactg gcaacacttc 7440
tcaacctgag acttacttca agatgctcga tcctggagag gtctatgatg accctattga 7500
cccaatcgag ttagaggctg aacccagagg aacccccatt gtccccaaca tcttgaggaa 7560
ctctgactac aatctcaact ctcctttgat agaagatcct gctagactaa tgttagaatg 7620
gttaaaaaca gggaatagac cttatcggat gactctaaca gacaattgct ccaggtcttt 7680
cagagttttg aaagattatt tcaagaaggt agatttgggt tctctcaagg tgggcggaat 7740
ggctgcacag tcaatgattt ctctctggtt atatggtgcc cactctgaat ccaacaggag 7800
ccggagatgt ataacagact tggcccattt ctattccaag tcgtccccca tagagaagct 7860
gttgaatctc acgctaggaa atagagggct gagaatcccc ccagagggag tgttaagttg 7920
ccttgagagg gttgattatg ataatgcatt tggaaggtat cttgccaaca cgtattcctc 7980
ttacttgttc ttccatgtaa tcaccttata catgaacgcc ctagactggg atgaagaaaa 8040
gaccatccta gcattatgga aagatttaac ctcagtggac atcgggaagg acttggtaaa 8100
gttcaaagac caaatatggg gactgctgat cgtgacaaag gactttgttt actcccaaag 8160
ttccaattgt ctttttgaca gaaactacac acttatgcta aaagatcttt tcttgtctcg 8220
cttcaactcc ttaatggtct tgctctctcc cccagagccc cgatactcag atgacttgat 8280
atctcaacta tgccagctgt acattgctgg ggatcaagtc ttgtctatgt gtggaaactc 8340
cggctatgaa gtcatcaaaa tattggagcc atatgtcgtg aatagtttag tccagagagc 8400
agaaaagttt aggcctctca ttcattcctt gggagacttt cctgtattta taaaagacaa 8460
ggtaagtcaa cttgaagaga cgttcggtcc ctgtgcaaga aggttcttta gggctctgga 8520
tcaattcgac aacatacatg acttggtttt tgtgtttggc tgttacaggc attgggggca 8580
cccatatata gattatcgaa agggtctgtc aaaactatat gatcaggttc accttaaaaa 8640
aatgatagat aagtcctacc aggagtgctt agcaagcgac ctagccagga ggatccttag 8700
atggggtttt gataagtact ccaagtggta tctggattca agattcctag cccgagacca 8760
ccccttgact ccttatatca aaacccaaac atggccaccc aaacatattg tagacttggt 8820
gggggataca tggcacaagc tcccgatcac gcagatcttt gagattcctg aatcaatgga 8880
tccgtcagaa atattggatg acaaatcaca ttctttcacc agaacgagac tagcttcttg 8940
gctgtcagaa aaccgagggg ggcctgttcc tagcgaaaaa gttattatca cggccctgtc 9000
taagccgcct gtcaatcccc gagagtttct gaggtctata gacctcggag gattgccaga 9060
tgaagacttg ataattggcc tcaagccaaa ggaacgggaa ttgaagattg aaggtcgatt 9120
ctttgctcta atgtcatgga atctaagatt gtattttgtc atcactgaaa aactcttggc 9180
caactacatc ttgccacttt ttgacgcgct gactatgaca gacaacctga acaaggtgtt 9240
taaaaagctg atcgacaggg tcaccgggca agggcttttg gactattcaa gggtcacata 9300
tgcatttcac ctggactatg aaaagtggaa caaccatcaa agattagagt caacagagga 9360
tgtattttct gtcctagatc aagtgtttgg attgaagaga gtgttttcta gaacacacga 9420
gttttttcaa aaggcctgga tctattattc agacagatca gacctcatcg ggttacggga 9480
ggatcaaata tactgcttag atgcgtccaa cggcccaacc tgttggaatg gccaggatgg 9540
cgggctagaa ggcttacggc agaagggctg gagtctagtc agcttattga tgatagatag 9600
agaatctcaa atcaggaaca caagaaccaa aatactagct caaggagaca accaggtttt 9660
atgtccgaca tacatgttgt cgccagggct atctcaagag gggctcctct atgaattgga 9720
gagaatatca aggaatgcac tttcgatata cagagccgtc gaggaagggg catctaagct 9780
agggctgatc atcaagaaag aagagaccat gtgtagttat gacttcctca tctatggaaa 9840
aacccctttg tttagaggta acatattggt gcctgagtcc aaaagatggg ccagagtctc 9900
ttgcgtctct aatgaccaaa tagtcaacct cgccaatata atgtcgacag tgtccaccaa 9960
tgcgctaaca gtggcacaac actctcaatc tttgatcaaa ccgatgaggg attttctgct 10020
catgtcagta caggcagtct ttcactacct gctatttagc ccaatcttaa agggaagagt 10080
ttacaagatt ctgagcgctg aaggggagag ctttctccta gccatgtcaa ggataatcta 10140
tctagatcct tctttgggag ggatatctgg aatgtccctc ggaagattcc atatacgaca 10200
gttctcagac cctgtctctg aagggttatc cttctggaga gagatctggt taagctccca 10260
agagtcctgg attcacgcgt tgtgtcaaga ggctggaaac ccagatcttg gagagagaac 10320
actcgagagc ttcactcgcc ttctagaaga tccgaccacc ttaaatatca gaggaggggc 10380
cagtcctacc attctactca aggatgcaat cagaaaggct ttatatgacg aggtggacaa 10440
ggtggaaaat tcagagtttc gagaggcaat cctgttgtcc aagacccata gagataattt 10500
tatactcttc ttaatatctg ttgagcctct gtttcctcga tttctcagtg agctattcag 10560
ttcgtctttt ttgggaatcc ccgagtcaat cattggattg atacaaaact cccgaacgat 10620
aagaaggcag tttagaaaga gtctctcaaa aactttagaa gaatccttct acaactcaga 10680
gatccacggg attagtcgga tgacccagac acctcagagg gttggggggg tgtggccttg 10740
ctcttcagag agggcagatc tacttaggga gatctcttgg ggaagaaaag tggtaggcac 10800
gacagttcct cacccttctg agatgttggg attacttccc aagtcctcta tttcttgcac 10860
ttgtggagca acaggaggag gcaatcctag agtttctgta tcagtactcc cgtcctttga 10920
tcagtcattt ttttcacgag gccccctaaa gggatacttg ggctcgtcca cctctatgtc 10980
gacccagcta ttccatgcat gggaaaaagt cactaatgtt catgtggtga agagagctct 11040
atcgttaaaa gaatctataa actggttcat tactagagat tccaacttgg ctcaagctct 11100
aattaggaac attatgtctc tgacaggccc tgatttccct ctagaggagg cccctgtctt 11160
caaaaggacg gggtcagcct tgcataggtt caagtctgcc agatacagcg aaggagggta 11220
ttcttctgtc tgcccgaacc tcctctctca tatttctgtt agtacagaca ccatgtctga 11280
tttgacccaa gacgggaaga actacgattt catgttccag ccattgatgc tttatgcaca 11340
gacatggaca tcagagctgg tacagagaga cacaaggcta agagactcta cgtttcattg 11400
gcacctccga tgcaacaggt gtgtgagacc cattgacgac gtgaccctgg agacctctca 11460
gatcttcgag tttccggatg tgtcgaaaag aatatccaga atggtttctg gggctgtgcc 11520
tcacttccag aggcttcccg atatccgtct gagaccagga gattttgaat ctctaagcgg 11580
tagagaaaag tctcaccata tcggatcagc tcaggggctc ttatactcaa tcttagtggc 11640
aattcacgac tcaggataca atgatggaac catcttccct gtcaacatat acggcaaggt 11700
ttcccctaga gactatttga gagggctcgc aaggggagta ttgataggat cctcgatttg 11760
cttcttgaca agaatgacaa atatcaatat taatagacct cttgaattgg tctcaggggt 11820
aatctcatat attctcctga ggctagataa ccatccctcc ttgtacataa tgctcagaga 11880
accgtctctt agaggagaga tattttctat ccctcagaaa atccccgccg cttatccaac 11940
cactatgaaa gaaggcaaca gatcaatctt gtgttatctc caacatgtgc tacgctatga 12000
gcgagagata atcacggcgt ctccagagaa tgactggcta tggatctttt cagactttag 12060
aagtgccaaa atgacgtacc tatccctcat tacttaccag tctcatcttc tactccagag 12120
ggttgagaga aacctatcta agagtatgag agataacctg cgacaattga gttctttgat 12180
gaggcaggtg ctgggcgggc acggagaaga taccttagag tcagacgaca acattcaacg 12240
actgctaaaa gactctttac gaaggacaag atgggtggat caagaggtgc gccatgcagc 12300
tagaaccatg actggagatt acagccccaa caagaaggtg tcccgtaagg taggatgttc 12360
agaatgggtc tgctctgctc aacaggttgc agtctctacc tcagcaaacc cggcccctgt 12420
ctcggagctt gacataaggg ccctctctaa gaggttccag aaccctttga tctcgggctt 12480
gagagtggtt cagtgggcaa ccggtgctca ttataagctt aagcctattc tagatgatct 12540
caatgttttc ccatctctct gccttgtagt tggggacggg tcagggggga tatcaagggc 12600
agtcctcaac atgtttccag atgccaagct tgtgttcaac agtcttttag aggtgaatga 12660
cctgatggct tccggaacac atccactgcc tccttcagca atcatgaggg gaggaaatga 12720
tatcgtctcc agagtgatag atcttgactc aatctgggaa aaaccgtccg acttgagaaa 12780
cttggcaacc tggaaatact tccagtcagt ccaaaagcag gtcaacatgt cctatgacct 12840
cattatttgc gatgcagaag ttactgacat tgcatctatc aaccggatca ccctgttaat 12900
gtccgatttt gcattgtcta tagatggacc actctatttg gtcttcaaaa cttatgggac 12960
tatgctagta aatccaaact acaaggctat tcaacacctg tcaagagcgt tcccctcggt 13020
cacagggttt atcacccaag taacttcgtc tttttcatct gagctctacc tccgattctc 13080
caaacgaggg aagtttttca gagatgctga gtacttgacc tcttccaccc ttcgagaaat 13140
gagccttgtg ttattcaatt gtagcagccc caagagtgag atgcagagag ctcgttcctt 13200
gaactatcag gatcttgtga gaggatttcc tgaagaaatc atatcaaatc cttacaatga 13260
gatgatcata actctgattg acagtgatgt agaatctttt ctagtccaca agatggttga 13320
tgatcttgag ttacagaggg gaactctgtc taaagtggct atcattatag ccatcatgat 13380
agttttctcc aacagagtct tcaacgtttc caaaccccta actgacccct cgttctatcc 13440
accgtctgat cccaaaatcc tgaggcactt caacatatgt tgcagtacta tgatgtatct 13500
atctactgct ttaggtgacg tccctagctt cgcaagactt cacgacctgt ataacagacc 13560
tataacttat tacttcagaa agcaagtcat tcgagggaac gtttatctat cttggagttg 13620
gtccaacgac acctcagtgt tcaaaagggt agcctgtaat tctagcctga gtctgtcatc 13680
tcactggatc aggttgattt acaagatagt gaagactacc agactcgttg gcagcatcaa 13740
ggatctatcc agagaagtgg aaagacacct tcataggtac aacaggtgga tcaccctaga 13800
ggatatcaga tctagatcat ccctactaga ctacagttgc ctgtgaaccg gatactcctg 13860
gaagcctgcc catgctaaga ctcttgtgtg atgtatcttg aaaaaaacaa gatcctaaat 13920
ctgaaccttt ggttgtttga ttgtttttct catttttgtt gtttatttgt taagcgt 13977
<210> 14
<211> 13923
<212> DNA
<213> 人工序列
<220>
<223> RABV载体: Coravax V5 China
<400> 14
acgcttaaca accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa 60
cacccctaca atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt 120
gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa 180
aaagccctgt ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt 240
gtcaggcatg agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc 300
aatgcagttt tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc 360
acgaaaagga gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga 420
agggaattgg gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca 480
tgcgtcctta gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa 540
cactggtaac tataagacaa acattgcaga caggatagag cagatttttg agacagcccc 600
ttttgttaaa atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg 660
gagtactata ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat 720
tgagcatcta tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc 780
aggactggta tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat 840
actatatttc ttccacaaga actttgagga agagataaga agaatgtttg agccagggca 900
ggagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa 960
atctccttat tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg 1020
ctatatgggt caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga 1080
aatgtctgtt ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag 1140
aagattcttc agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac 1200
tgacgtagca ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg 1260
tgaaaccaga agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa 1320
gagatctcac atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc 1380
attcgccgag tttctaaaca agacatattc gagtgactca taagaagttg aataacaaaa 1440
tgccggaaat ctacggattg tgtatatcca tcatgaaaaa aactaacacc cctcctttcg 1500
aaccatccca aacatgagca agatctttgt caatcctagt gctattagag ccggtctggc 1560
cgatcttgag atggctgaag aaactgttga tctgatcaat agaaatatcg aagacaatca 1620
ggctcatctc caaggggaac ccatagaggt ggacaatctc cctgaggata tggggcgact 1680
tcacctggat gatggaaaat cgcccaacca tggtgagata gccaaggtgg gagaaggcaa 1740
gtatcgagag gactttcaga tggatgaagg agaggatcct agcttcctgt tccagtcata 1800
cctggaaaat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gatttctcaa 1860
gatatggtca cagaccgtag aagagattat atcctatgtc gcggtcaact ttcccaaccc 1920
tccaggaaag tcttcagagg ataaatcaac ccagactact ggccgagagc tcaagaagga 1980
gacaacaccc actccttctc agagagaaag ccaatcatcg aaagccagga tggcggctca 2040
aattgcttct ggccctccag cccttgaatg gtcggctacc aatgaagagg atgatctatc 2100
agtggaggct gagatcgctc accagattgc agaaagtttc tccaaaaaat ataagtttcc 2160
ctctcgatcc tcagggatac tcttgtataa ttttgagcaa ttgaaaatga accttgatga 2220
tatagttaaa gaggcaaaaa atgtaccagg tgtgacccgt ttagcccatg acgggtccaa 2280
actcccccta agatgtgtac tgggatgggt cgctttggcc aactctaaga aattccagtt 2340
gttagtcgaa tccgacaagc tgagtaaaat catgcaagat gacttgaatc gctatacatc 2400
ttgctaaccg aacctctccc ctcagtccct ctagacaata aaatccgaga tgtcccaaag 2460
tcaacatgaa aaaaacaggc aacaccactg ataaaatgaa cctcctacgt aagatagtga 2520
aaaaccgcag ggacgaggac actcaaaaat cctctcccgc gtcagcccct ctggatgacg 2580
atgacttgtg gcttccaccc cctgaatacg tcccgctgaa agaacttaca ggcaagaaga 2640
acatgaggaa cttttgtatc aacggaaggg ttaaagtgtg tagcccgaat ggttactcgt 2700
tcaggatcct gcggcacatt ctgaaatcat tcgacgagat atattctggg aatcatagga 2760
tgatcgggtt agtcaaagtg gttattggac tggctttgtc aggatctcca gtccctgagg 2820
gcctgaactg ggtatacaaa ttgaggagaa cctttatctt ccagtgggct gattccaggg 2880
gccctcttga aggggaggag ttggaatact ctcaggagat cacttgggat gatgatactg 2940
agttcgtcgg attgcaaata agagtgattg caaaacagtg tcatatccag ggcagagtct 3000
ggtgtatcaa catgaacccg agagcatgtc aactatggtc tgacatgtct cttcagacac 3060
aaaggtccga agaggacaaa gattcctctc tgcttctaga ataatcagat tatatcccgc 3120
aaatttatca cttgtttacc tctggaggag agaacatatg ggctcaactc caacccttgg 3180
gagcaatata acaaaaaaca tgttatggtg ccattaaacc gctgcatttc atcaaagtca 3240
agttgattac ctttacattt tgatcctctt ggatgtgaaa aaaactatta acatccctca 3300
aaagaccccg ggccaccatg ttcgtgtttc tggtgctgct gcctctggtg agctcccagt 3360
gcgtgaacct gaccacaagg acccagctgc cccctgccta taccaattcc ttcacacggg 3420
gcgtgtacta tcccgacaag gtgttccgga gcagcgtgct gcactccaca caggatctgt 3480
ttctgccttt cttttctaac gtgacctggt tccacgccat ccacgtgagc ggcaccaatg 3540
gcacaaagcg gttcgacaat ccagtgctgc cctttaacga tggcgtgtac ttcgcctcca 3600
ccgagaagtc taacatcatc agaggctgga tctttggcac cacactggac agcaagacac 3660
agtccctgct gatcgtgaac aatgccacca acgtggtcat caaggtgtgc gagttccagt 3720
tttgtaatga tccattcctg ggcgtgtact atcacaagaa caataagtct tggatggaga 3780
gcgagtttcg cgtgtattcc tctgccaaca attgcacatt tgagtacgtg tcccagccct 3840
tcctgatgga cctggagggc aagcagggca atttcaagaa cctgagggag ttcgtgttta 3900
agaatatcga tggctacttc aaaatctact ccaagcacac cccaatcaac ctggtgcgcg 3960
acctgccaca gggcttctct gccctggagc cactggtgga tctgcccatc ggcatcaaca 4020
tcacccggtt tcagacactg ctggccctgc acagaagcta cctgacacca ggcgacagct 4080
cctctggatg gaccgcagga gcagcagcct actatgtggg ctatctgcag cccaggacct 4140
tcctgctgaa gtacaacgag aatggcacca tcacagacgc cgtggattgc gccctggatc 4200
ccctgtctga gaccaagtgt acactgaaga gctttaccgt ggagaagggc atctatcaga 4260
caagcaattt cagggtgcag cctaccgagt ccatcgtgcg ctttcccaat atcacaaacc 4320
tgtgcccttt tggcgaggtg ttcaacgcaa cccgcttcgc cagcgtgtac gcctggaata 4380
ggaagcgcat ctccaactgc gtggccgact attctgtgct gtacaacagc gcctccttct 4440
ctacctttaa gtgctatggc gtgagcccca caaagctgaa tgacctgtgc tttaccaacg 4500
tgtacgccga ttccttcgtg atcaggggcg acgaggtgcg ccagatcgca ccaggacaga 4560
caggcaagat cgcagactac aattataagc tgcctgacga tttcaccggc tgcgtgatcg 4620
cctggaactc taacaatctg gatagcaaag tgggcggcaa ctacaattat ctgtaccggc 4680
tgtttagaaa gtctaatctg aagccattcg agagggacat ctccacagaa atctaccagg 4740
ccggctctac cccctgcaat ggcgtggagg gctttaactg ttatttccct ctgcagagct 4800
acggcttcca gccaacaaac ggcgtgggct atcagcccta ccgcgtggtg gtgctgtctt 4860
ttgagctgct gcacgcacct gcaacagtgt gcggaccaaa gaagagcacc aatctggtga 4920
agaacaagtg cgtgaacttc aacttcaacg gactgaccgg aacaggcgtg ctgaccgagt 4980
ccaacaagaa gttcctgcct tttcagcagt tcggcaggga catcgcagat accacagacg 5040
ccgtgcgcga ccctcagacc ctggagatcc tggacatcac accatgctcc ttcggcggcg 5100
tgtctgtgat cacaccaggc accaatacaa gcaaccaggt ggccgtgctg tatcaggacg 5160
tgaattgtac cgaggtgcca gtggcaatcc acgcagatca gctgacccct acatggcggg 5220
tgtactctac cggcagcaac gtgttccaga caagagccgg atgcctgatc ggagcagagc 5280
acgtgaacaa tagctatgag tgcgacatcc ctatcggcgc cggcatctgt gcctcctacc 5340
agacccagac aaactcccca aggtctgtgg gagatgaggc cgaagacttt gtggaagtcc 5400
acctgcctga tgtgcataac caggtgtctg gcgtcgacct gggactgcca aattggggca 5460
agtacgtgct gctgagtgct ggagcactga ctgccctgat gctgatcatt ttcctgatga 5520
cctgctgtcg gcgcgtgaac agaagtgagc ccactcagca caatctgcga ggaaccggga 5580
gagaagtgtc agtcacacct cagagcggga aaatcattag tagttgggaa tcacataaaa 5640
gcgggggcga gaccaggctg ggatccggct ccggcgaggg caggggaagt ctactaacat 5700
gcggggacgt ggaggaaaat cccggcccca tggttcctca ggctctcctg tttgtacccc 5760
ttctggtttt tccattgtgt tttgggaaat tccctattta cacgatacca gacaagcttg 5820
gtccctggag tccgattgac atacatcacc tcagctgccc aaacaatttg gtagtggagg 5880
acgaaggatg caccaacctg tcagggttct cctacatgga acttaaagtt ggatacatct 5940
tagccataaa agtgaacggg ttcacttgca caggcgttgt gacggaggct gaaacctaca 6000
ctaacttcgt tggttatgtc acaaccacgt tcaaaagaaa gcatttccgc ccaacaccag 6060
atgcatgtag agccgcgtac aactggaaga tggccggtga ccccagatat gaagagtctc 6120
tacacaatcc gtaccctgac taccgctggc ttcgaactgt aaaaaccacc aaggagtctc 6180
tcgttatcat atctccaagt gtggcagatt tggacccata tgacagatcc cttcactcga 6240
gggtcttccc tagcgggaag tgctcaggag tagcggtgtc ttctacctac tgctccacta 6300
accacgatta caccatttgg atgcccgaga atccgagact agggatgtct tgtgacattt 6360
ttaccaatag tagagggaag agagcatcca aagggagtga gacttgcggc tttgtagatg 6420
aaagaggcct atataagtct ttaaaaggag catgcaaact caagttatgt ggagttctag 6480
gacttagact tatggatgga acatgggtct cgatgcaaac atcaaatgaa accaaatggt 6540
gccctcccga taagttggtg aacctgcacg actttcgctc agacgaaatt gagcaccttg 6600
ttgtagagga gttggtcagg aagagagagg agtgtctgga tgcactagag tccatcatga 6660
caaccaagtc agtgagtttc agacgtctca gtcatttaag aaaacttgtc cctgggtttg 6720
gaaaagcata taccatattc aacaagacct tgatggaagc cgatgctcac tacaagtcag 6780
tcgagacttg gaatgagatc ctcccttcaa aagggtgttt aagagttggg gggaggtgtc 6840
atcctcatgt gaacggggtg tttttcaatg gtataatatt aggacctgac ggcaatgtct 6900
taatcccaga gatgcaatca tccctcctcc agcaacatat ggagttgttg gaatcctcgg 6960
ttatccccct tgtgcacccc ctggcagacc cgtctaccgt tttcaaggac ggtgacgagg 7020
ctgaggattt tgttgaagtt caccttcccg atgtgcacaa tcaggtctca ggagttgact 7080
tgggtctccc gaactggggg aagtatgtat tactgagtgc aggggccctg actgccttga 7140
tgttgataat tttcctgatg acatgttgta gaagagtcaa tcgatcagaa cctacgcaac 7200
acaatctcag agggacaggg agggaggtgt cagtcactcc ccaaagcggg aagatcatat 7260
cttcatggga atcacacaag agtgggggtg agaccagact gtaagctagc ttataaagtg 7320
ctgggtcatc taagcttttc agtcgagaaa aaaacattag atcagaagaa caactggcaa 7380
cacttctcaa cctgagactt acttcaagat gctcgatcct ggagaggtct atgatgaccc 7440
tattgaccca atcgagttag aggctgaacc cagaggaacc cccattgtcc ccaacatctt 7500
gaggaactct gactacaatc tcaactctcc tttgatagaa gatcctgcta gactaatgtt 7560
agaatggtta aaaacaggga atagacctta tcggatgact ctaacagaca attgctccag 7620
gtctttcaga gttttgaaag attatttcaa gaaggtagat ttgggttctc tcaaggtggg 7680
cggaatggct gcacagtcaa tgatttctct ctggttatat ggtgcccact ctgaatccaa 7740
caggagccgg agatgtataa cagacttggc ccatttctat tccaagtcgt cccccataga 7800
gaagctgttg aatctcacgc taggaaatag agggctgaga atccccccag agggagtgtt 7860
aagttgcctt gagagggttg attatgataa tgcatttgga aggtatcttg ccaacacgta 7920
ttcctcttac ttgttcttcc atgtaatcac cttatacatg aacgccctag actgggatga 7980
agaaaagacc atcctagcat tatggaaaga tttaacctca gtggacatcg ggaaggactt 8040
ggtaaagttc aaagaccaaa tatggggact gctgatcgtg acaaaggact ttgtttactc 8100
ccaaagttcc aattgtcttt ttgacagaaa ctacacactt atgctaaaag atcttttctt 8160
gtctcgcttc aactccttaa tggtcttgct ctctccccca gagccccgat actcagatga 8220
cttgatatct caactatgcc agctgtacat tgctggggat caagtcttgt ctatgtgtgg 8280
aaactccggc tatgaagtca tcaaaatatt ggagccatat gtcgtgaata gtttagtcca 8340
gagagcagaa aagtttaggc ctctcattca ttccttggga gactttcctg tatttataaa 8400
agacaaggta agtcaacttg aagagacgtt cggtccctgt gcaagaaggt tctttagggc 8460
tctggatcaa ttcgacaaca tacatgactt ggtttttgtg tttggctgtt acaggcattg 8520
ggggcaccca tatatagatt atcgaaaggg tctgtcaaaa ctatatgatc aggttcacct 8580
taaaaaaatg atagataagt cctaccagga gtgcttagca agcgacctag ccaggaggat 8640
ccttagatgg ggttttgata agtactccaa gtggtatctg gattcaagat tcctagcccg 8700
agaccacccc ttgactcctt atatcaaaac ccaaacatgg ccacccaaac atattgtaga 8760
cttggtgggg gatacatggc acaagctccc gatcacgcag atctttgaga ttcctgaatc 8820
aatggatccg tcagaaatat tggatgacaa atcacattct ttcaccagaa cgagactagc 8880
ttcttggctg tcagaaaacc gaggggggcc tgttcctagc gaaaaagtta ttatcacggc 8940
cctgtctaag ccgcctgtca atccccgaga gtttctgagg tctatagacc tcggaggatt 9000
gccagatgaa gacttgataa ttggcctcaa gccaaaggaa cgggaattga agattgaagg 9060
tcgattcttt gctctaatgt catggaatct aagattgtat tttgtcatca ctgaaaaact 9120
cttggccaac tacatcttgc cactttttga cgcgctgact atgacagaca acctgaacaa 9180
ggtgtttaaa aagctgatcg acagggtcac cgggcaaggg cttttggact attcaagggt 9240
cacatatgca tttcacctgg actatgaaaa gtggaacaac catcaaagat tagagtcaac 9300
agaggatgta ttttctgtcc tagatcaagt gtttggattg aagagagtgt tttctagaac 9360
acacgagttt tttcaaaagg cctggatcta ttattcagac agatcagacc tcatcgggtt 9420
acgggaggat caaatatact gcttagatgc gtccaacggc ccaacctgtt ggaatggcca 9480
ggatggcggg ctagaaggct tacggcagaa gggctggagt ctagtcagct tattgatgat 9540
agatagagaa tctcaaatca ggaacacaag aaccaaaata ctagctcaag gagacaacca 9600
ggttttatgt ccgacataca tgttgtcgcc agggctatct caagaggggc tcctctatga 9660
attggagaga atatcaagga atgcactttc gatatacaga gccgtcgagg aaggggcatc 9720
taagctaggg ctgatcatca agaaagaaga gaccatgtgt agttatgact tcctcatcta 9780
tggaaaaacc cctttgttta gaggtaacat attggtgcct gagtccaaaa gatgggccag 9840
agtctcttgc gtctctaatg accaaatagt caacctcgcc aatataatgt cgacagtgtc 9900
caccaatgcg ctaacagtgg cacaacactc tcaatctttg atcaaaccga tgagggattt 9960
tctgctcatg tcagtacagg cagtctttca ctacctgcta tttagcccaa tcttaaaggg 10020
aagagtttac aagattctga gcgctgaagg ggagagcttt ctcctagcca tgtcaaggat 10080
aatctatcta gatccttctt tgggagggat atctggaatg tccctcggaa gattccatat 10140
acgacagttc tcagaccctg tctctgaagg gttatccttc tggagagaga tctggttaag 10200
ctcccaagag tcctggattc acgcgttgtg tcaagaggct ggaaacccag atcttggaga 10260
gagaacactc gagagcttca ctcgccttct agaagatccg accaccttaa atatcagagg 10320
aggggccagt cctaccattc tactcaagga tgcaatcaga aaggctttat atgacgaggt 10380
ggacaaggtg gaaaattcag agtttcgaga ggcaatcctg ttgtccaaga cccatagaga 10440
taattttata ctcttcttaa tatctgttga gcctctgttt cctcgatttc tcagtgagct 10500
attcagttcg tcttttttgg gaatccccga gtcaatcatt ggattgatac aaaactcccg 10560
aacgataaga aggcagttta gaaagagtct ctcaaaaact ttagaagaat ccttctacaa 10620
ctcagagatc cacgggatta gtcggatgac ccagacacct cagagggttg ggggggtgtg 10680
gccttgctct tcagagaggg cagatctact tagggagatc tcttggggaa gaaaagtggt 10740
aggcacgaca gttcctcacc cttctgagat gttgggatta cttcccaagt cctctatttc 10800
ttgcacttgt ggagcaacag gaggaggcaa tcctagagtt tctgtatcag tactcccgtc 10860
ctttgatcag tcattttttt cacgaggccc cctaaaggga tacttgggct cgtccacctc 10920
tatgtcgacc cagctattcc atgcatggga aaaagtcact aatgttcatg tggtgaagag 10980
agctctatcg ttaaaagaat ctataaactg gttcattact agagattcca acttggctca 11040
agctctaatt aggaacatta tgtctctgac aggccctgat ttccctctag aggaggcccc 11100
tgtcttcaaa aggacggggt cagccttgca taggttcaag tctgccagat acagcgaagg 11160
agggtattct tctgtctgcc cgaacctcct ctctcatatt tctgttagta cagacaccat 11220
gtctgatttg acccaagacg ggaagaacta cgatttcatg ttccagccat tgatgcttta 11280
tgcacagaca tggacatcag agctggtaca gagagacaca aggctaagag actctacgtt 11340
tcattggcac ctccgatgca acaggtgtgt gagacccatt gacgacgtga ccctggagac 11400
ctctcagatc ttcgagtttc cggatgtgtc gaaaagaata tccagaatgg tttctggggc 11460
tgtgcctcac ttccagaggc ttcccgatat ccgtctgaga ccaggagatt ttgaatctct 11520
aagcggtaga gaaaagtctc accatatcgg atcagctcag gggctcttat actcaatctt 11580
agtggcaatt cacgactcag gatacaatga tggaaccatc ttccctgtca acatatacgg 11640
caaggtttcc cctagagact atttgagagg gctcgcaagg ggagtattga taggatcctc 11700
gatttgcttc ttgacaagaa tgacaaatat caatattaat agacctcttg aattggtctc 11760
aggggtaatc tcatatattc tcctgaggct agataaccat ccctccttgt acataatgct 11820
cagagaaccg tctcttagag gagagatatt ttctatccct cagaaaatcc ccgccgctta 11880
tccaaccact atgaaagaag gcaacagatc aatcttgtgt tatctccaac atgtgctacg 11940
ctatgagcga gagataatca cggcgtctcc agagaatgac tggctatgga tcttttcaga 12000
ctttagaagt gccaaaatga cgtacctatc cctcattact taccagtctc atcttctact 12060
ccagagggtt gagagaaacc tatctaagag tatgagagat aacctgcgac aattgagttc 12120
tttgatgagg caggtgctgg gcgggcacgg agaagatacc ttagagtcag acgacaacat 12180
tcaacgactg ctaaaagact ctttacgaag gacaagatgg gtggatcaag aggtgcgcca 12240
tgcagctaga accatgactg gagattacag ccccaacaag aaggtgtccc gtaaggtagg 12300
atgttcagaa tgggtctgct ctgctcaaca ggttgcagtc tctacctcag caaacccggc 12360
ccctgtctcg gagcttgaca taagggccct ctctaagagg ttccagaacc ctttgatctc 12420
gggcttgaga gtggttcagt gggcaaccgg tgctcattat aagcttaagc ctattctaga 12480
tgatctcaat gttttcccat ctctctgcct tgtagttggg gacgggtcag gggggatatc 12540
aagggcagtc ctcaacatgt ttccagatgc caagcttgtg ttcaacagtc ttttagaggt 12600
gaatgacctg atggcttccg gaacacatcc actgcctcct tcagcaatca tgaggggagg 12660
aaatgatatc gtctccagag tgatagatct tgactcaatc tgggaaaaac cgtccgactt 12720
gagaaacttg gcaacctgga aatacttcca gtcagtccaa aagcaggtca acatgtccta 12780
tgacctcatt atttgcgatg cagaagttac tgacattgca tctatcaacc ggatcaccct 12840
gttaatgtcc gattttgcat tgtctataga tggaccactc tatttggtct tcaaaactta 12900
tgggactatg ctagtaaatc caaactacaa ggctattcaa cacctgtcaa gagcgttccc 12960
ctcggtcaca gggtttatca cccaagtaac ttcgtctttt tcatctgagc tctacctccg 13020
attctccaaa cgagggaagt ttttcagaga tgctgagtac ttgacctctt ccacccttcg 13080
agaaatgagc cttgtgttat tcaattgtag cagccccaag agtgagatgc agagagctcg 13140
ttccttgaac tatcaggatc ttgtgagagg atttcctgaa gaaatcatat caaatcctta 13200
caatgagatg atcataactc tgattgacag tgatgtagaa tcttttctag tccacaagat 13260
ggttgatgat cttgagttac agaggggaac tctgtctaaa gtggctatca ttatagccat 13320
catgatagtt ttctccaaca gagtcttcaa cgtttccaaa cccctaactg acccctcgtt 13380
ctatccaccg tctgatccca aaatcctgag gcacttcaac atatgttgca gtactatgat 13440
gtatctatct actgctttag gtgacgtccc tagcttcgca agacttcacg acctgtataa 13500
cagacctata acttattact tcagaaagca agtcattcga gggaacgttt atctatcttg 13560
gagttggtcc aacgacacct cagtgttcaa aagggtagcc tgtaattcta gcctgagtct 13620
gtcatctcac tggatcaggt tgatttacaa gatagtgaag actaccagac tcgttggcag 13680
catcaaggat ctatccagag aagtggaaag acaccttcat aggtacaaca ggtggatcac 13740
cctagaggat atcagatcta gatcatccct actagactac agttgcctgt gaaccggata 13800
ctcctggaag cctgcccatg ctaagactct tgtgtgatgt atcttgaaaa aaacaagatc 13860
ctaaatctga acctttggtt gtttgattgt ttttctcatt tttgttgttt atttgttaag 13920
cgt 13923
<210> 15
<211> 13974
<212> DNA
<213> 人工序列
<220>
<223> RABV载体: Coravax V5 South Africa
<400> 15
acgcttaaca accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa 60
cacccctaca atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt 120
gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa 180
aaagccctgt ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt 240
gtcaggcatg agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc 300
aatgcagttt tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc 360
acgaaaagga gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga 420
agggaattgg gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca 480
tgcgtcctta gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa 540
cactggtaac tataagacaa acattgcaga caggatagag cagatttttg agacagcccc 600
ttttgttaaa atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg 660
gagtactata ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat 720
tgagcatcta tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc 780
aggactggta tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat 840
actatatttc ttccacaaga actttgagga agagataaga agaatgtttg agccagggca 900
ggagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa 960
atctccttat tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg 1020
ctatatgggt caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga 1080
aatgtctgtt ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag 1140
aagattcttc agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac 1200
tgacgtagca ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg 1260
tgaaaccaga agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa 1320
gagatctcac atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc 1380
attcgccgag tttctaaaca agacatattc gagtgactca taagaagttg aataacaaaa 1440
tgccggaaat ctacggattg tgtatatcca tcatgaaaaa aactaacacc cctcctttcg 1500
aaccatccca aacatgagca agatctttgt caatcctagt gctattagag ccggtctggc 1560
cgatcttgag atggctgaag aaactgttga tctgatcaat agaaatatcg aagacaatca 1620
ggctcatctc caaggggaac ccatagaggt ggacaatctc cctgaggata tggggcgact 1680
tcacctggat gatggaaaat cgcccaacca tggtgagata gccaaggtgg gagaaggcaa 1740
gtatcgagag gactttcaga tggatgaagg agaggatcct agcttcctgt tccagtcata 1800
cctggaaaat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gatttctcaa 1860
gatatggtca cagaccgtag aagagattat atcctatgtc gcggtcaact ttcccaaccc 1920
tccaggaaag tcttcagagg ataaatcaac ccagactact ggccgagagc tcaagaagga 1980
gacaacaccc actccttctc agagagaaag ccaatcatcg aaagccagga tggcggctca 2040
aattgcttct ggccctccag cccttgaatg gtcggctacc aatgaagagg atgatctatc 2100
agtggaggct gagatcgctc accagattgc agaaagtttc tccaaaaaat ataagtttcc 2160
ctctcgatcc tcagggatac tcttgtataa ttttgagcaa ttgaaaatga accttgatga 2220
tatagttaaa gaggcaaaaa atgtaccagg tgtgacccgt ttagcccatg acgggtccaa 2280
actcccccta agatgtgtac tgggatgggt cgctttggcc aactctaaga aattccagtt 2340
gttagtcgaa tccgacaagc tgagtaaaat catgcaagat gacttgaatc gctatacatc 2400
ttgctaaccg aacctctccc ctcagtccct ctagacaata aaatccgaga tgtcccaaag 2460
tcaacatgaa aaaaacaggc aacaccactg ataaaatgaa cctcctacgt aagatagtga 2520
aaaaccgcag ggacgaggac actcaaaaat cctctcccgc gtcagcccct ctggatgacg 2580
atgacttgtg gcttccaccc cctgaatacg tcccgctgaa agaacttaca ggcaagaaga 2640
acatgaggaa cttttgtatc aacggaaggg ttaaagtgtg tagcccgaat ggttactcgt 2700
tcaggatcct gcggcacatt ctgaaatcat tcgacgagat atattctggg aatcatagga 2760
tgatcgggtt agtcaaagtg gttattggac tggctttgtc aggatctcca gtccctgagg 2820
gcctgaactg ggtatacaaa ttgaggagaa cctttatctt ccagtgggct gattccaggg 2880
gccctcttga aggggaggag ttggaatact ctcaggagat cacttgggat gatgatactg 2940
agttcgtcgg attgcaaata agagtgattg caaaacagtg tcatatccag ggcagagtct 3000
ggtgtatcaa catgaacccg agagcatgtc aactatggtc tgacatgtct cttcagacac 3060
aaaggtccga agaggacaaa gattcctctc tgcttctaga ataatcagat tatatcccgc 3120
aaatttatca cttgtttacc tctggaggag agaacatatg ggctcaactc caacccttgg 3180
gagcaatata acaaaaaaca tgttatggtg ccattaaacc gctgcatttc atcaaagtca 3240
agttgattac ctttacattt tgatcctctt ggatgtgaaa aaaactatta acatccctca 3300
aaagaccccg ggccaccatg ttcgtgtttc tggtgctgct gcctctggtg agctcccagt 3360
gcgtgaactt caccacaagg acccagctgc cccctgccta taccaattcc ttcacacggg 3420
gcgtgtacta tcccgacaag gtgttccgga gcagcgtgct gcactccaca caggatctgt 3480
ttctgccttt cttttctaac gtgacctggt tccacgccat ccacgtgagc ggcaccaatg 3540
gcacaaagcg gttcgccaat ccagtgctgc cctttaacga tggcgtgtac ttcgcctcca 3600
ccgagaagtc taacatcatc agaggctgga tctttggcac cacactggac agcaagacac 3660
agtccctgct gatcgtgaac aatgccacca acgtggtcat caaggtgtgc gagttccagt 3720
tttgtaatga tccattcctg ggcgtgtact atcacaagaa caataagtct tggatggaga 3780
gcgagtttcg cgtgtattcc tctgccaaca attgcacatt tgagtacgtg tcccagccct 3840
tcctgatgga cctggagggc aagcagggca atttcaagaa cctgagggag ttcgtgttta 3900
agaatatcga tggctacttc aaaatctact ccaagcacac cccaatcaac ctggtgcgcg 3960
gcctgccaca gggcttctct gccctggagc cactggtgga tctgcccatc ggcatcaaca 4020
tcacccggtt tcagacactg ctggccctgc acagaagcta cctgacacca ggcgacagct 4080
cctctggatg gaccgcagga gcagcagcct actatgtggg ctatctgcag cccaggacct 4140
tcctgctgaa gtacaacgag aatggcacca tcacagacgc cgtggattgc gccctggatc 4200
ccctgtctga gaccaagtgt acactgaaga gctttaccgt ggagaagggc atctatcaga 4260
caagcaattt cagggtgcag cctaccgagt ccatcgtgcg ctttcccaat atcacaaacc 4320
tgtgcccttt tggcgaggtg ttcaacgcaa cccgcttcgc cagcgtgtac gcctggaata 4380
ggaagcgcat ctccaactgc gtggccgact attctgtgct gtacaacagc gcctccttct 4440
ctacctttaa gtgctatggc gtgagcccca caaagctgaa tgacctgtgc tttaccaacg 4500
tgtacgccga ttccttcgtg atcaggggcg acgaggtgcg ccagatcgca ccaggacaga 4560
caggcaatat cgcagactac aattataagc tgcctgacga tttcaccggc tgcgtgatcg 4620
cctggaactc taacaatctg gatagcaaag tgggcggcaa ctacaattat ctgtaccggc 4680
tgtttagaaa gtctaatctg aagccattcg agagggacat ctccacagaa atctaccagg 4740
ccggctctac cccctgcaat ggcgtgaagg gctttaactg ttatttccct ctgcagagct 4800
acggcttcca gccaacatat ggcgtgggct atcagcccta ccgcgtggtg gtgctgtctt 4860
ttgagctgct gcacgcacct gcaacagtgt gcggaccaaa gaagagcacc aatctggtga 4920
agaacaagtg cgtgaacttc aacttcaacg gactgaccgg aacaggcgtg ctgaccgagt 4980
ccaacaagaa gttcctgcct tttcagcagt tcggcaggga catcgcagat accacagacg 5040
ccgtgcgcga ccctcagacc ctggagatcc tggacatcac accatgctcc ttcggcggcg 5100
tgtctgtgat cacaccaggc accaatacaa gcaaccaggt ggccgtgctg tatcagggcg 5160
tgaattgtac cgaggtgcca gtggcaatcc acgcagatca gctgacccct acatggcggg 5220
tgtactctac cggcagcaac gtgttccaga caagagccgg atgcctgatc ggagcagagc 5280
acgtgaacaa tagctatgag tgcgacatcc ctatcggcgc cggcatctgt gcctcctacc 5340
agacccagac aaactcccca gaatcaagcg tgattcctct ggtccatcca ctggcagatc 5400
cctccacagt gttcaaagac ggagatgagg ccgaagactt tgtggaagtc cacctgcctg 5460
atgtgcataa ccaggtgtct ggcgtcgacc tgggactgcc aaattggggc aagtacgtgc 5520
tgctgagtgc tggagcactg actgccctga tgctgatcat tttcctgatg acctgctgtc 5580
ggcgcgtgaa cagaagtgag cccactcagc acaatctgcg aggaaccggg agagaagtgt 5640
cagtcacacc tcagagcggg aaaatcatta gtagttggga atcacataaa agcgggggcg 5700
agaccaggct gggatccggc tccggcgagg gcaggggaag tctactaaca tgcggggacg 5760
tggaggaaaa tcccggcccc atggttcctc aggctctcct gtttgtaccc cttctggttt 5820
ttccattgtg ttttgggaaa ttccctattt acacgatacc agacaagctt ggtccctgga 5880
gtccgattga catacatcac ctcagctgcc caaacaattt ggtagtggag gacgaaggat 5940
gcaccaacct gtcagggttc tcctacatgg aacttaaagt tggatacatc ttagccataa 6000
aagtgaacgg gttcacttgc acaggcgttg tgacggaggc tgaaacctac actaacttcg 6060
ttggttatgt cacaaccacg ttcaaaagaa agcatttccg cccaacacca gatgcatgta 6120
gagccgcgta caactggaag atggccggtg accccagata tgaagagtct ctacacaatc 6180
cgtaccctga ctaccgctgg cttcgaactg taaaaaccac caaggagtct ctcgttatca 6240
tatctccaag tgtggcagat ttggacccat atgacagatc ccttcactcg agggtcttcc 6300
ctagcgggaa gtgctcagga gtagcggtgt cttctaccta ctgctccact aaccacgatt 6360
acaccatttg gatgcccgag aatccgagac tagggatgtc ttgtgacatt tttaccaata 6420
gtagagggaa gagagcatcc aaagggagtg agacttgcgg ctttgtagat gaaagaggcc 6480
tatataagtc tttaaaagga gcatgcaaac tcaagttatg tggagttcta ggacttagac 6540
ttatggatgg aacatgggtc tcgatgcaaa catcaaatga aaccaaatgg tgccctcccg 6600
ataagttggt gaacctgcac gactttcgct cagacgaaat tgagcacctt gttgtagagg 6660
agttggtcag gaagagagag gagtgtctgg atgcactaga gtccatcatg acaaccaagt 6720
cagtgagttt cagacgtctc agtcatttaa gaaaacttgt ccctgggttt ggaaaagcat 6780
ataccatatt caacaagacc ttgatggaag ccgatgctca ctacaagtca gtcgagactt 6840
ggaatgagat cctcccttca aaagggtgtt taagagttgg ggggaggtgt catcctcatg 6900
tgaacggggt gtttttcaat ggtataatat taggacctga cggcaatgtc ttaatcccag 6960
agatgcaatc atccctcctc cagcaacata tggagttgtt ggaatcctcg gttatccccc 7020
ttgtgcaccc cctggcagac ccgtctaccg ttttcaagga cggtgacgag gctgaggatt 7080
ttgttgaagt tcaccttccc gatgtgcaca atcaggtctc aggagttgac ttgggtctcc 7140
cgaactgggg gaagtatgta ttactgagtg caggggccct gactgccttg atgttgataa 7200
ttttcctgat gacatgttgt agaagagtca atcgatcaga acctacgcaa cacaatctca 7260
gagggacagg gagggaggtg tcagtcactc cccaaagcgg gaagatcata tcttcatggg 7320
aatcacacaa gagtgggggt gagaccagac tgtaagctag cttataaagt gctgggtcat 7380
ctaagctttt cagtcgagaa aaaaacatta gatcagaaga acaactggca acacttctca 7440
acctgagact tacttcaaga tgctcgatcc tggagaggtc tatgatgacc ctattgaccc 7500
aatcgagtta gaggctgaac ccagaggaac ccccattgtc cccaacatct tgaggaactc 7560
tgactacaat ctcaactctc ctttgataga agatcctgct agactaatgt tagaatggtt 7620
aaaaacaggg aatagacctt atcggatgac tctaacagac aattgctcca ggtctttcag 7680
agttttgaaa gattatttca agaaggtaga tttgggttct ctcaaggtgg gcggaatggc 7740
tgcacagtca atgatttctc tctggttata tggtgcccac tctgaatcca acaggagccg 7800
gagatgtata acagacttgg cccatttcta ttccaagtcg tcccccatag agaagctgtt 7860
gaatctcacg ctaggaaata gagggctgag aatcccccca gagggagtgt taagttgcct 7920
tgagagggtt gattatgata atgcatttgg aaggtatctt gccaacacgt attcctctta 7980
cttgttcttc catgtaatca ccttatacat gaacgcccta gactgggatg aagaaaagac 8040
catcctagca ttatggaaag atttaacctc agtggacatc gggaaggact tggtaaagtt 8100
caaagaccaa atatggggac tgctgatcgt gacaaaggac tttgtttact cccaaagttc 8160
caattgtctt tttgacagaa actacacact tatgctaaaa gatcttttct tgtctcgctt 8220
caactcctta atggtcttgc tctctccccc agagccccga tactcagatg acttgatatc 8280
tcaactatgc cagctgtaca ttgctgggga tcaagtcttg tctatgtgtg gaaactccgg 8340
ctatgaagtc atcaaaatat tggagccata tgtcgtgaat agtttagtcc agagagcaga 8400
aaagtttagg cctctcattc attccttggg agactttcct gtatttataa aagacaaggt 8460
aagtcaactt gaagagacgt tcggtccctg tgcaagaagg ttctttaggg ctctggatca 8520
attcgacaac atacatgact tggtttttgt gtttggctgt tacaggcatt gggggcaccc 8580
atatatagat tatcgaaagg gtctgtcaaa actatatgat caggttcacc ttaaaaaaat 8640
gatagataag tcctaccagg agtgcttagc aagcgaccta gccaggagga tccttagatg 8700
gggttttgat aagtactcca agtggtatct ggattcaaga ttcctagccc gagaccaccc 8760
cttgactcct tatatcaaaa cccaaacatg gccacccaaa catattgtag acttggtggg 8820
ggatacatgg cacaagctcc cgatcacgca gatctttgag attcctgaat caatggatcc 8880
gtcagaaata ttggatgaca aatcacattc tttcaccaga acgagactag cttcttggct 8940
gtcagaaaac cgaggggggc ctgttcctag cgaaaaagtt attatcacgg ccctgtctaa 9000
gccgcctgtc aatccccgag agtttctgag gtctatagac ctcggaggat tgccagatga 9060
agacttgata attggcctca agccaaagga acgggaattg aagattgaag gtcgattctt 9120
tgctctaatg tcatggaatc taagattgta ttttgtcatc actgaaaaac tcttggccaa 9180
ctacatcttg ccactttttg acgcgctgac tatgacagac aacctgaaca aggtgtttaa 9240
aaagctgatc gacagggtca ccgggcaagg gcttttggac tattcaaggg tcacatatgc 9300
atttcacctg gactatgaaa agtggaacaa ccatcaaaga ttagagtcaa cagaggatgt 9360
attttctgtc ctagatcaag tgtttggatt gaagagagtg ttttctagaa cacacgagtt 9420
ttttcaaaag gcctggatct attattcaga cagatcagac ctcatcgggt tacgggagga 9480
tcaaatatac tgcttagatg cgtccaacgg cccaacctgt tggaatggcc aggatggcgg 9540
gctagaaggc ttacggcaga agggctggag tctagtcagc ttattgatga tagatagaga 9600
atctcaaatc aggaacacaa gaaccaaaat actagctcaa ggagacaacc aggttttatg 9660
tccgacatac atgttgtcgc cagggctatc tcaagagggg ctcctctatg aattggagag 9720
aatatcaagg aatgcacttt cgatatacag agccgtcgag gaaggggcat ctaagctagg 9780
gctgatcatc aagaaagaag agaccatgtg tagttatgac ttcctcatct atggaaaaac 9840
ccctttgttt agaggtaaca tattggtgcc tgagtccaaa agatgggcca gagtctcttg 9900
cgtctctaat gaccaaatag tcaacctcgc caatataatg tcgacagtgt ccaccaatgc 9960
gctaacagtg gcacaacact ctcaatcttt gatcaaaccg atgagggatt ttctgctcat 10020
gtcagtacag gcagtctttc actacctgct atttagccca atcttaaagg gaagagttta 10080
caagattctg agcgctgaag gggagagctt tctcctagcc atgtcaagga taatctatct 10140
agatccttct ttgggaggga tatctggaat gtccctcgga agattccata tacgacagtt 10200
ctcagaccct gtctctgaag ggttatcctt ctggagagag atctggttaa gctcccaaga 10260
gtcctggatt cacgcgttgt gtcaagaggc tggaaaccca gatcttggag agagaacact 10320
cgagagcttc actcgccttc tagaagatcc gaccacctta aatatcagag gaggggccag 10380
tcctaccatt ctactcaagg atgcaatcag aaaggcttta tatgacgagg tggacaaggt 10440
ggaaaattca gagtttcgag aggcaatcct gttgtccaag acccatagag ataattttat 10500
actcttctta atatctgttg agcctctgtt tcctcgattt ctcagtgagc tattcagttc 10560
gtcttttttg ggaatccccg agtcaatcat tggattgata caaaactccc gaacgataag 10620
aaggcagttt agaaagagtc tctcaaaaac tttagaagaa tccttctaca actcagagat 10680
ccacgggatt agtcggatga cccagacacc tcagagggtt gggggggtgt ggccttgctc 10740
ttcagagagg gcagatctac ttagggagat ctcttgggga agaaaagtgg taggcacgac 10800
agttcctcac ccttctgaga tgttgggatt acttcccaag tcctctattt cttgcacttg 10860
tggagcaaca ggaggaggca atcctagagt ttctgtatca gtactcccgt cctttgatca 10920
gtcatttttt tcacgaggcc ccctaaaggg atacttgggc tcgtccacct ctatgtcgac 10980
ccagctattc catgcatggg aaaaagtcac taatgttcat gtggtgaaga gagctctatc 11040
gttaaaagaa tctataaact ggttcattac tagagattcc aacttggctc aagctctaat 11100
taggaacatt atgtctctga caggccctga tttccctcta gaggaggccc ctgtcttcaa 11160
aaggacgggg tcagccttgc ataggttcaa gtctgccaga tacagcgaag gagggtattc 11220
ttctgtctgc ccgaacctcc tctctcatat ttctgttagt acagacacca tgtctgattt 11280
gacccaagac gggaagaact acgatttcat gttccagcca ttgatgcttt atgcacagac 11340
atggacatca gagctggtac agagagacac aaggctaaga gactctacgt ttcattggca 11400
cctccgatgc aacaggtgtg tgagacccat tgacgacgtg accctggaga cctctcagat 11460
cttcgagttt ccggatgtgt cgaaaagaat atccagaatg gtttctgggg ctgtgcctca 11520
cttccagagg cttcccgata tccgtctgag accaggagat tttgaatctc taagcggtag 11580
agaaaagtct caccatatcg gatcagctca ggggctctta tactcaatct tagtggcaat 11640
tcacgactca ggatacaatg atggaaccat cttccctgtc aacatatacg gcaaggtttc 11700
ccctagagac tatttgagag ggctcgcaag gggagtattg ataggatcct cgatttgctt 11760
cttgacaaga atgacaaata tcaatattaa tagacctctt gaattggtct caggggtaat 11820
ctcatatatt ctcctgaggc tagataacca tccctccttg tacataatgc tcagagaacc 11880
gtctcttaga ggagagatat tttctatccc tcagaaaatc cccgccgctt atccaaccac 11940
tatgaaagaa ggcaacagat caatcttgtg ttatctccaa catgtgctac gctatgagcg 12000
agagataatc acggcgtctc cagagaatga ctggctatgg atcttttcag actttagaag 12060
tgccaaaatg acgtacctat ccctcattac ttaccagtct catcttctac tccagagggt 12120
tgagagaaac ctatctaaga gtatgagaga taacctgcga caattgagtt ctttgatgag 12180
gcaggtgctg ggcgggcacg gagaagatac cttagagtca gacgacaaca ttcaacgact 12240
gctaaaagac tctttacgaa ggacaagatg ggtggatcaa gaggtgcgcc atgcagctag 12300
aaccatgact ggagattaca gccccaacaa gaaggtgtcc cgtaaggtag gatgttcaga 12360
atgggtctgc tctgctcaac aggttgcagt ctctacctca gcaaacccgg cccctgtctc 12420
ggagcttgac ataagggccc tctctaagag gttccagaac cctttgatct cgggcttgag 12480
agtggttcag tgggcaaccg gtgctcatta taagcttaag cctattctag atgatctcaa 12540
tgttttccca tctctctgcc ttgtagttgg ggacgggtca ggggggatat caagggcagt 12600
cctcaacatg tttccagatg ccaagcttgt gttcaacagt cttttagagg tgaatgacct 12660
gatggcttcc ggaacacatc cactgcctcc ttcagcaatc atgaggggag gaaatgatat 12720
cgtctccaga gtgatagatc ttgactcaat ctgggaaaaa ccgtccgact tgagaaactt 12780
ggcaacctgg aaatacttcc agtcagtcca aaagcaggtc aacatgtcct atgacctcat 12840
tatttgcgat gcagaagtta ctgacattgc atctatcaac cggatcaccc tgttaatgtc 12900
cgattttgca ttgtctatag atggaccact ctatttggtc ttcaaaactt atgggactat 12960
gctagtaaat ccaaactaca aggctattca acacctgtca agagcgttcc cctcggtcac 13020
agggtttatc acccaagtaa cttcgtcttt ttcatctgag ctctacctcc gattctccaa 13080
acgagggaag tttttcagag atgctgagta cttgacctct tccacccttc gagaaatgag 13140
ccttgtgtta ttcaattgta gcagccccaa gagtgagatg cagagagctc gttccttgaa 13200
ctatcaggat cttgtgagag gatttcctga agaaatcata tcaaatcctt acaatgagat 13260
gatcataact ctgattgaca gtgatgtaga atcttttcta gtccacaaga tggttgatga 13320
tcttgagtta cagaggggaa ctctgtctaa agtggctatc attatagcca tcatgatagt 13380
tttctccaac agagtcttca acgtttccaa acccctaact gacccctcgt tctatccacc 13440
gtctgatccc aaaatcctga ggcacttcaa catatgttgc agtactatga tgtatctatc 13500
tactgcttta ggtgacgtcc ctagcttcgc aagacttcac gacctgtata acagacctat 13560
aacttattac ttcagaaagc aagtcattcg agggaacgtt tatctatctt ggagttggtc 13620
caacgacacc tcagtgttca aaagggtagc ctgtaattct agcctgagtc tgtcatctca 13680
ctggatcagg ttgatttaca agatagtgaa gactaccaga ctcgttggca gcatcaagga 13740
tctatccaga gaagtggaaa gacaccttca taggtacaac aggtggatca ccctagagga 13800
tatcagatct agatcatccc tactagacta cagttgcctg tgaaccggat actcctggaa 13860
gcctgcccat gctaagactc ttgtgtgatg tatcttgaaa aaaacaagat cctaaatctg 13920
aacctttggt tgtttgattg tttttctcat ttttgttgtt tatttgttaa gcgt 13974
<210> 16
<211> 13881
<212> DNA
<213> 人工序列
<220>
<223> RABV载体: Coravax V6 China
<400> 16
acgcttaaca accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa 60
cacccctaca atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt 120
gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa 180
aaagccctgt ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt 240
gtcaggcatg agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc 300
aatgcagttt tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc 360
acgaaaagga gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga 420
agggaattgg gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca 480
tgcgtcctta gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa 540
cactggtaac tataagacaa acattgcaga caggatagag cagatttttg agacagcccc 600
ttttgttaaa atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg 660
gagtactata ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat 720
tgagcatcta tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc 780
aggactggta tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat 840
actatatttc ttccacaaga actttgagga agagataaga agaatgtttg agccagggca 900
ggagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa 960
atctccttat tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg 1020
ctatatgggt caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga 1080
aatgtctgtt ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag 1140
aagattcttc agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac 1200
tgacgtagca ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg 1260
tgaaaccaga agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa 1320
gagatctcac atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc 1380
attcgccgag tttctaaaca agacatattc gagtgactca taagaagttg aataacaaaa 1440
tgccggaaat ctacggattg tgtatatcca tcatgaaaaa aactaacacc cctcctttcg 1500
aaccatccca aacatgagca agatctttgt caatcctagt gctattagag ccggtctggc 1560
cgatcttgag atggctgaag aaactgttga tctgatcaat agaaatatcg aagacaatca 1620
ggctcatctc caaggggaac ccatagaggt ggacaatctc cctgaggata tggggcgact 1680
tcacctggat gatggaaaat cgcccaacca tggtgagata gccaaggtgg gagaaggcaa 1740
gtatcgagag gactttcaga tggatgaagg agaggatcct agcttcctgt tccagtcata 1800
cctggaaaat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gatttctcaa 1860
gatatggtca cagaccgtag aagagattat atcctatgtc gcggtcaact ttcccaaccc 1920
tccaggaaag tcttcagagg ataaatcaac ccagactact ggccgagagc tcaagaagga 1980
gacaacaccc actccttctc agagagaaag ccaatcatcg aaagccagga tggcggctca 2040
aattgcttct ggccctccag cccttgaatg gtcggctacc aatgaagagg atgatctatc 2100
agtggaggct gagatcgctc accagattgc agaaagtttc tccaaaaaat ataagtttcc 2160
ctctcgatcc tcagggatac tcttgtataa ttttgagcaa ttgaaaatga accttgatga 2220
tatagttaaa gaggcaaaaa atgtaccagg tgtgacccgt ttagcccatg acgggtccaa 2280
actcccccta agatgtgtac tgggatgggt cgctttggcc aactctaaga aattccagtt 2340
gttagtcgaa tccgacaagc tgagtaaaat catgcaagat gacttgaatc gctatacatc 2400
ttgctaaccg aacctctccc ctcagtccct ctagacaata aaatccgaga tgtcccaaag 2460
tcaacatgaa aaaaacaggc aacaccactg ataaaatgaa cctcctacgt aagatagtga 2520
aaaaccgcag ggacgaggac actcaaaaat cctctcccgc gtcagcccct ctggatgacg 2580
atgacttgtg gcttccaccc cctgaatacg tcccgctgaa agaacttaca ggcaagaaga 2640
acatgaggaa cttttgtatc aacggaaggg ttaaagtgtg tagcccgaat ggttactcgt 2700
tcaggatcct gcggcacatt ctgaaatcat tcgacgagat atattctggg aatcatagga 2760
tgatcgggtt agtcaaagtg gttattggac tggctttgtc aggatctcca gtccctgagg 2820
gcctgaactg ggtatacaaa ttgaggagaa cctttatctt ccagtgggct gattccaggg 2880
gccctcttga aggggaggag ttggaatact ctcaggagat cacttgggat gatgatactg 2940
agttcgtcgg attgcaaata agagtgattg caaaacagtg tcatatccag ggcagagtct 3000
ggtgtatcaa catgaacccg agagcatgtc aactatggtc tgacatgtct cttcagacac 3060
aaaggtccga agaggacaaa gattcctctc tgcttctaga ataatcagat tatatcccgc 3120
aaatttatca cttgtttacc tctggaggag agaacatatg ggctcaactc caacccttgg 3180
gagcaatata acaaaaaaca tgttatggtg ccattaaacc gctgcatttc atcaaagtca 3240
agttgattac ctttacattt tgatcctctt ggatgtgaaa aaaactatta acatccctca 3300
aaagaccccg ggaaagatgg ttcctcaggc tctcctgttt gtaccccttc tggtttttcc 3360
attgtgtttt gggaaattcc ctatttacac gataccagac aagcttggtc cctggagtcc 3420
gattgacata catcacctca gctgcccaaa caatttggta gtggaggacg aaggatgcac 3480
caacctgtca gggttctcct acatggaact taaagttgga tacatcttag ccataaaagt 3540
gaacgggttc acttgcacag gcgttgtgac ggaggctgaa acctacacta acttcgttgg 3600
ttatgtcaca accacgttca aaagaaagca tttccgccca acaccagatg catgtagagc 3660
cgcgtacaac tggaagatgg ccggtgaccc cagatatgaa gagtctctac acaatccgta 3720
ccctgactac cgctggcttc gaactgtaaa aaccaccaag gagtctctcg ttatcatatc 3780
tccaagtgtg gcagatttgg acccatatga cagatccctt cactcgaggg tcttccctag 3840
cgggaagtgc tcaggagtag cggtgtcttc tacctactgc tccactaacc acgattacac 3900
catttggatg cccgagaatc cgagactagg gatgtcttgt gacattttta ccaatagtag 3960
agggaagaga gcatccaaag ggagtgagac ttgcggcttt gtagatgaaa gaggcctata 4020
taagtcttta aaaggagcat gcaaactcaa gttatgtgga gttctaggac ttagacttat 4080
ggatggaaca tgggtctcga tgcaaacatc aaatgaaacc aaatggtgcc ctcccgataa 4140
gttggtgaac ctgcacgact ttcgctcaga cgaaattgag caccttgttg tagaggagtt 4200
ggtcaggaag agagaggagt gtctggatgc actagagtcc atcatgacaa ccaagtcagt 4260
gagtttcaga cgtctcagtc atttaagaaa acttgtccct gggtttggaa aagcatatac 4320
catattcaac aagaccttga tggaagccga tgctcactac aagtcagtcg agacttggaa 4380
tgagatcctc ccttcaaaag ggtgtttaag agttgggggg aggtgtcatc ctcatgtgaa 4440
cggggtgttt ttcaatggta taatattagg acctgacggc aatgtcttaa tcccagagat 4500
gcaatcatcc ctcctccagc aacatatgga gttgttggaa tcctcggtta tcccccttgt 4560
gcaccccctg gcagacccgt ctaccgtttt caaggacggt gacgaggctg aggattttgt 4620
tgaagttcac cttcccgatg tgcacaatca ggtctcagga gttgacttgg gtctcccgaa 4680
ctgggggaag tatgtattac tgagtgcagg ggccctgact gccttgatgt tgataatttt 4740
cctgatgaca tgttgtagaa gagtcaatcg atcagaacct acgcaacaca atctcagagg 4800
gacagggagg gaggtgtcag tcactcccca aagcgggaag atcatatctt catgggaatc 4860
acacaagagt gggggtgaga ccagactgta attaattaac gtcctttcaa cgatccaagt 4920
ccatgaaaaa aactaacacc cctcccgtac gaccatgttc gtgtttctgg tgctgctgcc 4980
tctggtgagc tcccagtgcg tgaacctgac cacaaggacc cagctgcccc ctgcctatac 5040
caattccttc acacggggcg tgtactatcc cgacaaggtg ttccggagca gcgtgctgca 5100
ctccacacag gatctgtttc tgcctttctt ttctaacgtg acctggttcc acgccatcca 5160
cgtgagcggc accaatggca caaagcggtt cgacaatcca gtgctgccct ttaacgatgg 5220
cgtgtacttc gcctccaccg agaagtctaa catcatcaga ggctggatct ttggcaccac 5280
actggacagc aagacacagt ccctgctgat cgtgaacaat gccaccaacg tggtcatcaa 5340
ggtgtgcgag ttccagtttt gtaatgatcc attcctgggc gtgtactatc acaagaacaa 5400
taagtcttgg atggagagcg agtttcgcgt gtattcctct gccaacaatt gcacatttga 5460
gtacgtgtcc cagcccttcc tgatggacct ggagggcaag cagggcaatt tcaagaacct 5520
gagggagttc gtgtttaaga atatcgatgg ctacttcaaa atctactcca agcacacccc 5580
aatcaacctg gtgcgcgacc tgccacaggg cttctctgcc ctggagccac tggtggatct 5640
gcccatcggc atcaacatca cccggtttca gacactgctg gccctgcaca gaagctacct 5700
gacaccaggc gacagctcct ctggatggac cgcaggagca gcagcctact atgtgggcta 5760
tctgcagccc aggaccttcc tgctgaagta caacgagaat ggcaccatca cagacgccgt 5820
ggattgcgcc ctggatcccc tgtctgagac caagtgtaca ctgaagagct ttaccgtgga 5880
gaagggcatc tatcagacaa gcaatttcag ggtgcagcct accgagtcca tcgtgcgctt 5940
tcccaatatc acaaacctgt gcccttttgg cgaggtgttc aacgcaaccc gcttcgccag 6000
cgtgtacgcc tggaatagga agcgcatctc caactgcgtg gccgactatt ctgtgctgta 6060
caacagcgcc tccttctcta cctttaagtg ctatggcgtg agccccacaa agctgaatga 6120
cctgtgcttt accaacgtgt acgccgattc cttcgtgatc aggggcgacg aggtgcgcca 6180
gatcgcacca ggacagacag gcaagatcgc agactacaat tataagctgc ctgacgattt 6240
caccggctgc gtgatcgcct ggaactctaa caatctggat agcaaagtgg gcggcaacta 6300
caattatctg taccggctgt ttagaaagtc taatctgaag ccattcgaga gggacatctc 6360
cacagaaatc taccaggccg gctctacccc ctgcaatggc gtggagggct ttaactgtta 6420
tttccctctg cagagctacg gcttccagcc aacaaacggc gtgggctatc agccctaccg 6480
cgtggtggtg ctgtcttttg agctgctgca cgcacctgca acagtgtgcg gaccaaagaa 6540
gagcaccaat ctggtgaaga acaagtgcgt gaacttcaac ttcaacggac tgaccggaac 6600
aggcgtgctg accgagtcca acaagaagtt cctgcctttt cagcagttcg gcagggacat 6660
cgcagatacc acagacgccg tgcgcgaccc tcagaccctg gagatcctgg acatcacacc 6720
atgctccttc ggcggcgtgt ctgtgatcac accaggcacc aatacaagca accaggtggc 6780
cgtgctgtat caggacgtga attgtaccga ggtgccagtg gcaatccacg cagatcagct 6840
gacccctaca tggcgggtgt actctaccgg cagcaacgtg ttccagacaa gagccggatg 6900
cctgatcgga gcagagcacg tgaacaatag ctatgagtgc gacatcccta tcggcgccgg 6960
catctgtgcc tcctaccaga cccagacaaa ctccccaagg tctgtgggag atgaggccga 7020
agactttgtg gaagtccacc tgcctgatgt gcataaccag gtgtctggcg tcgacctggg 7080
actgccaaat tggggcaagt acgtgctgct gagtgctgga gcactgactg ccctgatgct 7140
gatcattttc ctgatgacct gctgtcggcg cgtgaacaga agtgagccca ctcagcacaa 7200
tctgcgagga accgggagag aagtgtcagt cacacctcag agcgggaaaa tcattagtag 7260
ttgggaatca cataaaagcg ggggcgagac caggctggga tccggctccg gcgagggcag 7320
gggaagtcta ctaacatgcg gggacgtgga ggaaaatccc ggccccatgc tcgatcctgg 7380
agaggtctat gatgacccta ttgacccaat cgagttagag gctgaaccca gaggaacccc 7440
cattgtcccc aacatcttga ggaactctga ctacaatctc aactctcctt tgatagaaga 7500
tcctgctaga ctaatgttag aatggttaaa aacagggaat agaccttatc ggatgactct 7560
aacagacaat tgctccaggt ctttcagagt tttgaaagat tatttcaaga aggtagattt 7620
gggttctctc aaggtgggcg gaatggctgc acagtcaatg atttctctct ggttatatgg 7680
tgcccactct gaatccaaca ggagccggag atgtataaca gacttggccc atttctattc 7740
caagtcgtcc cccatagaga agctgttgaa tctcacgcta ggaaatagag ggctgagaat 7800
ccccccagag ggagtgttaa gttgccttga gagggttgat tatgataatg catttggaag 7860
gtatcttgcc aacacgtatt cctcttactt gttcttccat gtaatcacct tatacatgaa 7920
cgccctagac tgggatgaag aaaagaccat cctagcatta tggaaagatt taacctcagt 7980
ggacatcggg aaggacttgg taaagttcaa agaccaaata tggggactgc tgatcgtgac 8040
aaaggacttt gtttactccc aaagttccaa ttgtcttttt gacagaaact acacacttat 8100
gctaaaagat cttttcttgt ctcgcttcaa ctccttaatg gtcttgctct ctcccccaga 8160
gccccgatac tcagatgact tgatatctca actatgccag ctgtacattg ctggggatca 8220
agtcttgtct atgtgtggaa actccggcta tgaagtcatc aaaatattgg agccatatgt 8280
cgtgaatagt ttagtccaga gagcagaaaa gtttaggcct ctcattcatt ccttgggaga 8340
ctttcctgta tttataaaag acaaggtaag tcaacttgaa gagacgttcg gtccctgtgc 8400
aagaaggttc tttagggctc tggatcaatt cgacaacata catgacttgg tttttgtgtt 8460
tggctgttac aggcattggg ggcacccata tatagattat cgaaagggtc tgtcaaaact 8520
atatgatcag gttcacctta aaaaaatgat agataagtcc taccaggagt gcttagcaag 8580
cgacctagcc aggaggatcc ttagatgggg ttttgataag tactccaagt ggtatctgga 8640
ttcaagattc ctagcccgag accacccctt gactccttat atcaaaaccc aaacatggcc 8700
acccaaacat attgtagact tggtggggga tacatggcac aagctcccga tcacgcagat 8760
ctttgagatt cctgaatcaa tggatccgtc agaaatattg gatgacaaat cacattcttt 8820
caccagaacg agactagctt cttggctgtc agaaaaccga ggggggcctg ttcctagcga 8880
aaaagttatt atcacggccc tgtctaagcc gcctgtcaat ccccgagagt ttctgaggtc 8940
tatagacctc ggaggattgc cagatgaaga cttgataatt ggcctcaagc caaaggaacg 9000
ggaattgaag attgaaggtc gattctttgc tctaatgtca tggaatctaa gattgtattt 9060
tgtcatcact gaaaaactct tggccaacta catcttgcca ctttttgacg cgctgactat 9120
gacagacaac ctgaacaagg tgtttaaaaa gctgatcgac agggtcaccg ggcaagggct 9180
tttggactat tcaagggtca catatgcatt tcacctggac tatgaaaagt ggaacaacca 9240
tcaaagatta gagtcaacag aggatgtatt ttctgtccta gatcaagtgt ttggattgaa 9300
gagagtgttt tctagaacac acgagttttt tcaaaaggcc tggatctatt attcagacag 9360
atcagacctc atcgggttac gggaggatca aatatactgc ttagatgcgt ccaacggccc 9420
aacctgttgg aatggccagg atggcgggct agaaggctta cggcagaagg gctggagtct 9480
agtcagctta ttgatgatag atagagaatc tcaaatcagg aacacaagaa ccaaaatact 9540
agctcaagga gacaaccagg ttttatgtcc gacatacatg ttgtcgccag ggctatctca 9600
agaggggctc ctctatgaat tggagagaat atcaaggaat gcactttcga tatacagagc 9660
cgtcgaggaa ggggcatcta agctagggct gatcatcaag aaagaagaga ccatgtgtag 9720
ttatgacttc ctcatctatg gaaaaacccc tttgtttaga ggtaacatat tggtgcctga 9780
gtccaaaaga tgggccagag tctcttgcgt ctctaatgac caaatagtca acctcgccaa 9840
tataatgtcg acagtgtcca ccaatgcgct aacagtggca caacactctc aatctttgat 9900
caaaccgatg agggattttc tgctcatgtc agtacaggca gtctttcact acctgctatt 9960
tagcccaatc ttaaagggaa gagtttacaa gattctgagc gctgaagggg agagctttct 10020
cctagccatg tcaaggataa tctatctaga tccttctttg ggagggatat ctggaatgtc 10080
cctcggaaga ttccatatac gacagttctc agaccctgtc tctgaagggt tatccttctg 10140
gagagagatc tggttaagct cccaagagtc ctggattcac gcgttgtgtc aagaggctgg 10200
aaacccagat cttggagaga gaacactcga gagcttcact cgccttctag aagatccgac 10260
caccttaaat atcagaggag gggccagtcc taccattcta ctcaaggatg caatcagaaa 10320
ggctttatat gacgaggtgg acaaggtgga aaattcagag tttcgagagg caatcctgtt 10380
gtccaagacc catagagata attttatact cttcttaata tctgttgagc ctctgtttcc 10440
tcgatttctc agtgagctat tcagttcgtc ttttttggga atccccgagt caatcattgg 10500
attgatacaa aactcccgaa cgataagaag gcagtttaga aagagtctct caaaaacttt 10560
agaagaatcc ttctacaact cagagatcca cgggattagt cggatgaccc agacacctca 10620
gagggttggg ggggtgtggc cttgctcttc agagagggca gatctactta gggagatctc 10680
ttggggaaga aaagtggtag gcacgacagt tcctcaccct tctgagatgt tgggattact 10740
tcccaagtcc tctatttctt gcacttgtgg agcaacagga ggaggcaatc ctagagtttc 10800
tgtatcagta ctcccgtcct ttgatcagtc atttttttca cgaggccccc taaagggata 10860
cttgggctcg tccacctcta tgtcgaccca gctattccat gcatgggaaa aagtcactaa 10920
tgttcatgtg gtgaagagag ctctatcgtt aaaagaatct ataaactggt tcattactag 10980
agattccaac ttggctcaag ctctaattag gaacattatg tctctgacag gccctgattt 11040
ccctctagag gaggcccctg tcttcaaaag gacggggtca gccttgcata ggttcaagtc 11100
tgccagatac agcgaaggag ggtattcttc tgtctgcccg aacctcctct ctcatatttc 11160
tgttagtaca gacaccatgt ctgatttgac ccaagacggg aagaactacg atttcatgtt 11220
ccagccattg atgctttatg cacagacatg gacatcagag ctggtacaga gagacacaag 11280
gctaagagac tctacgtttc attggcacct ccgatgcaac aggtgtgtga gacccattga 11340
cgacgtgacc ctggagacct ctcagatctt cgagtttccg gatgtgtcga aaagaatatc 11400
cagaatggtt tctggggctg tgcctcactt ccagaggctt cccgatatcc gtctgagacc 11460
aggagatttt gaatctctaa gcggtagaga aaagtctcac catatcggat cagctcaggg 11520
gctcttatac tcaatcttag tggcaattca cgactcagga tacaatgatg gaaccatctt 11580
ccctgtcaac atatacggca aggtttcccc tagagactat ttgagagggc tcgcaagggg 11640
agtattgata ggatcctcga tttgcttctt gacaagaatg acaaatatca atattaatag 11700
acctcttgaa ttggtctcag gggtaatctc atatattctc ctgaggctag ataaccatcc 11760
ctccttgtac ataatgctca gagaaccgtc tcttagagga gagatatttt ctatccctca 11820
gaaaatcccc gccgcttatc caaccactat gaaagaaggc aacagatcaa tcttgtgtta 11880
tctccaacat gtgctacgct atgagcgaga gataatcacg gcgtctccag agaatgactg 11940
gctatggatc ttttcagact ttagaagtgc caaaatgacg tacctatccc tcattactta 12000
ccagtctcat cttctactcc agagggttga gagaaaccta tctaagagta tgagagataa 12060
cctgcgacaa ttgagttctt tgatgaggca ggtgctgggc gggcacggag aagatacctt 12120
agagtcagac gacaacattc aacgactgct aaaagactct ttacgaagga caagatgggt 12180
ggatcaagag gtgcgccatg cagctagaac catgactgga gattacagcc ccaacaagaa 12240
ggtgtcccgt aaggtaggat gttcagaatg ggtctgctct gctcaacagg ttgcagtctc 12300
tacctcagca aacccggccc ctgtctcgga gcttgacata agggccctct ctaagaggtt 12360
ccagaaccct ttgatctcgg gcttgagagt ggttcagtgg gcaaccggtg ctcattataa 12420
gcttaagcct attctagatg atctcaatgt tttcccatct ctctgccttg tagttgggga 12480
cgggtcaggg gggatatcaa gggcagtcct caacatgttt ccagatgcca agcttgtgtt 12540
caacagtctt ttagaggtga atgacctgat ggcttccgga acacatccac tgcctccttc 12600
agcaatcatg aggggaggaa atgatatcgt ctccagagtg atagatcttg actcaatctg 12660
ggaaaaaccg tccgacttga gaaacttggc aacctggaaa tacttccagt cagtccaaaa 12720
gcaggtcaac atgtcctatg acctcattat ttgcgatgca gaagttactg acattgcatc 12780
tatcaaccgg atcaccctgt taatgtccga ttttgcattg tctatagatg gaccactcta 12840
tttggtcttc aaaacttatg ggactatgct agtaaatcca aactacaagg ctattcaaca 12900
cctgtcaaga gcgttcccct cggtcacagg gtttatcacc caagtaactt cgtctttttc 12960
atctgagctc tacctccgat tctccaaacg agggaagttt ttcagagatg ctgagtactt 13020
gacctcttcc acccttcgag aaatgagcct tgtgttattc aattgtagca gccccaagag 13080
tgagatgcag agagctcgtt ccttgaacta tcaggatctt gtgagaggat ttcctgaaga 13140
aatcatatca aatccttaca atgagatgat cataactctg attgacagtg atgtagaatc 13200
ttttctagtc cacaagatgg ttgatgatct tgagttacag aggggaactc tgtctaaagt 13260
ggctatcatt atagccatca tgatagtttt ctccaacaga gtcttcaacg tttccaaacc 13320
cctaactgac ccctcgttct atccaccgtc tgatcccaaa atcctgaggc acttcaacat 13380
atgttgcagt actatgatgt atctatctac tgctttaggt gacgtcccta gcttcgcaag 13440
acttcacgac ctgtataaca gacctataac ttattacttc agaaagcaag tcattcgagg 13500
gaacgtttat ctatcttgga gttggtccaa cgacacctca gtgttcaaaa gggtagcctg 13560
taattctagc ctgagtctgt catctcactg gatcaggttg atttacaaga tagtgaagac 13620
taccagactc gttggcagca tcaaggatct atccagagaa gtggaaagac accttcatag 13680
gtacaacagg tggatcaccc tagaggatat cagatctaga tcatccctac tagactacag 13740
ttgcctgtga accggatact cctggaagcc tgcccatgct aagactcttg tgtgatgtat 13800
cttgaaaaaa acaagatcct aaatctgaac ctttggttgt ttgattgttt ttctcatttt 13860
tgttgtttat ttgttaagcg t 13881
<210> 17
<211> 13932
<212> DNA
<213> 人工序列
<220>
<223> RABV载体: Coravax V6 South Africa
<400> 17
acgcttaaca accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa 60
cacccctaca atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt 120
gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa 180
aaagccctgt ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt 240
gtcaggcatg agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc 300
aatgcagttt tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc 360
acgaaaagga gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga 420
agggaattgg gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca 480
tgcgtcctta gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa 540
cactggtaac tataagacaa acattgcaga caggatagag cagatttttg agacagcccc 600
ttttgttaaa atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg 660
gagtactata ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat 720
tgagcatcta tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc 780
aggactggta tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat 840
actatatttc ttccacaaga actttgagga agagataaga agaatgtttg agccagggca 900
ggagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa 960
atctccttat tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg 1020
ctatatgggt caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga 1080
aatgtctgtt ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag 1140
aagattcttc agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac 1200
tgacgtagca ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg 1260
tgaaaccaga agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa 1320
gagatctcac atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc 1380
attcgccgag tttctaaaca agacatattc gagtgactca taagaagttg aataacaaaa 1440
tgccggaaat ctacggattg tgtatatcca tcatgaaaaa aactaacacc cctcctttcg 1500
aaccatccca aacatgagca agatctttgt caatcctagt gctattagag ccggtctggc 1560
cgatcttgag atggctgaag aaactgttga tctgatcaat agaaatatcg aagacaatca 1620
ggctcatctc caaggggaac ccatagaggt ggacaatctc cctgaggata tggggcgact 1680
tcacctggat gatggaaaat cgcccaacca tggtgagata gccaaggtgg gagaaggcaa 1740
gtatcgagag gactttcaga tggatgaagg agaggatcct agcttcctgt tccagtcata 1800
cctggaaaat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gatttctcaa 1860
gatatggtca cagaccgtag aagagattat atcctatgtc gcggtcaact ttcccaaccc 1920
tccaggaaag tcttcagagg ataaatcaac ccagactact ggccgagagc tcaagaagga 1980
gacaacaccc actccttctc agagagaaag ccaatcatcg aaagccagga tggcggctca 2040
aattgcttct ggccctccag cccttgaatg gtcggctacc aatgaagagg atgatctatc 2100
agtggaggct gagatcgctc accagattgc agaaagtttc tccaaaaaat ataagtttcc 2160
ctctcgatcc tcagggatac tcttgtataa ttttgagcaa ttgaaaatga accttgatga 2220
tatagttaaa gaggcaaaaa atgtaccagg tgtgacccgt ttagcccatg acgggtccaa 2280
actcccccta agatgtgtac tgggatgggt cgctttggcc aactctaaga aattccagtt 2340
gttagtcgaa tccgacaagc tgagtaaaat catgcaagat gacttgaatc gctatacatc 2400
ttgctaaccg aacctctccc ctcagtccct ctagacaata aaatccgaga tgtcccaaag 2460
tcaacatgaa aaaaacaggc aacaccactg ataaaatgaa cctcctacgt aagatagtga 2520
aaaaccgcag ggacgaggac actcaaaaat cctctcccgc gtcagcccct ctggatgacg 2580
atgacttgtg gcttccaccc cctgaatacg tcccgctgaa agaacttaca ggcaagaaga 2640
acatgaggaa cttttgtatc aacggaaggg ttaaagtgtg tagcccgaat ggttactcgt 2700
tcaggatcct gcggcacatt ctgaaatcat tcgacgagat atattctggg aatcatagga 2760
tgatcgggtt agtcaaagtg gttattggac tggctttgtc aggatctcca gtccctgagg 2820
gcctgaactg ggtatacaaa ttgaggagaa cctttatctt ccagtgggct gattccaggg 2880
gccctcttga aggggaggag ttggaatact ctcaggagat cacttgggat gatgatactg 2940
agttcgtcgg attgcaaata agagtgattg caaaacagtg tcatatccag ggcagagtct 3000
ggtgtatcaa catgaacccg agagcatgtc aactatggtc tgacatgtct cttcagacac 3060
aaaggtccga agaggacaaa gattcctctc tgcttctaga ataatcagat tatatcccgc 3120
aaatttatca cttgtttacc tctggaggag agaacatatg ggctcaactc caacccttgg 3180
gagcaatata acaaaaaaca tgttatggtg ccattaaacc gctgcatttc atcaaagtca 3240
agttgattac ctttacattt tgatcctctt ggatgtgaaa aaaactatta acatccctca 3300
aaagaccccg ggaaagatgg ttcctcaggc tctcctgttt gtaccccttc tggtttttcc 3360
attgtgtttt gggaaattcc ctatttacac gataccagac aagcttggtc cctggagtcc 3420
gattgacata catcacctca gctgcccaaa caatttggta gtggaggacg aaggatgcac 3480
caacctgtca gggttctcct acatggaact taaagttgga tacatcttag ccataaaagt 3540
gaacgggttc acttgcacag gcgttgtgac ggaggctgaa acctacacta acttcgttgg 3600
ttatgtcaca accacgttca aaagaaagca tttccgccca acaccagatg catgtagagc 3660
cgcgtacaac tggaagatgg ccggtgaccc cagatatgaa gagtctctac acaatccgta 3720
ccctgactac cgctggcttc gaactgtaaa aaccaccaag gagtctctcg ttatcatatc 3780
tccaagtgtg gcagatttgg acccatatga cagatccctt cactcgaggg tcttccctag 3840
cgggaagtgc tcaggagtag cggtgtcttc tacctactgc tccactaacc acgattacac 3900
catttggatg cccgagaatc cgagactagg gatgtcttgt gacattttta ccaatagtag 3960
agggaagaga gcatccaaag ggagtgagac ttgcggcttt gtagatgaaa gaggcctata 4020
taagtcttta aaaggagcat gcaaactcaa gttatgtgga gttctaggac ttagacttat 4080
ggatggaaca tgggtctcga tgcaaacatc aaatgaaacc aaatggtgcc ctcccgataa 4140
gttggtgaac ctgcacgact ttcgctcaga cgaaattgag caccttgttg tagaggagtt 4200
ggtcaggaag agagaggagt gtctggatgc actagagtcc atcatgacaa ccaagtcagt 4260
gagtttcaga cgtctcagtc atttaagaaa acttgtccct gggtttggaa aagcatatac 4320
catattcaac aagaccttga tggaagccga tgctcactac aagtcagtcg agacttggaa 4380
tgagatcctc ccttcaaaag ggtgtttaag agttgggggg aggtgtcatc ctcatgtgaa 4440
cggggtgttt ttcaatggta taatattagg acctgacggc aatgtcttaa tcccagagat 4500
gcaatcatcc ctcctccagc aacatatgga gttgttggaa tcctcggtta tcccccttgt 4560
gcaccccctg gcagacccgt ctaccgtttt caaggacggt gacgaggctg aggattttgt 4620
tgaagttcac cttcccgatg tgcacaatca ggtctcagga gttgacttgg gtctcccgaa 4680
ctgggggaag tatgtattac tgagtgcagg ggccctgact gccttgatgt tgataatttt 4740
cctgatgaca tgttgtagaa gagtcaatcg atcagaacct acgcaacaca atctcagagg 4800
gacagggagg gaggtgtcag tcactcccca aagcgggaag atcatatctt catgggaatc 4860
acacaagagt gggggtgaga ccagactgta attaattaac gtcctttcaa cgatccaagt 4920
ccatgaaaaa aactaacacc cctcccgtac gaccatgttc gtgtttctgg tgctgctgcc 4980
tctggtgagc tcccagtgcg tgaacttcac cacaaggacc cagctgcccc ctgcctatac 5040
caattccttc acacggggcg tgtactatcc cgacaaggtg ttccggagca gcgtgctgca 5100
ctccacacag gatctgtttc tgcctttctt ttctaacgtg acctggttcc acgccatcca 5160
cgtgagcggc accaatggca caaagcggtt cgccaatcca gtgctgccct ttaacgatgg 5220
cgtgtacttc gcctccaccg agaagtctaa catcatcaga ggctggatct ttggcaccac 5280
actggacagc aagacacagt ccctgctgat cgtgaacaat gccaccaacg tggtcatcaa 5340
ggtgtgcgag ttccagtttt gtaatgatcc attcctgggc gtgtactatc acaagaacaa 5400
taagtcttgg atggagagcg agtttcgcgt gtattcctct gccaacaatt gcacatttga 5460
gtacgtgtcc cagcccttcc tgatggacct ggagggcaag cagggcaatt tcaagaacct 5520
gagggagttc gtgtttaaga atatcgatgg ctacttcaaa atctactcca agcacacccc 5580
aatcaacctg gtgcgcggcc tgccacaggg cttctctgcc ctggagccac tggtggatct 5640
gcccatcggc atcaacatca cccggtttca gacactgctg gccctgcaca gaagctacct 5700
gacaccaggc gacagctcct ctggatggac cgcaggagca gcagcctact atgtgggcta 5760
tctgcagccc aggaccttcc tgctgaagta caacgagaat ggcaccatca cagacgccgt 5820
ggattgcgcc ctggatcccc tgtctgagac caagtgtaca ctgaagagct ttaccgtgga 5880
gaagggcatc tatcagacaa gcaatttcag ggtgcagcct accgagtcca tcgtgcgctt 5940
tcccaatatc acaaacctgt gcccttttgg cgaggtgttc aacgcaaccc gcttcgccag 6000
cgtgtacgcc tggaatagga agcgcatctc caactgcgtg gccgactatt ctgtgctgta 6060
caacagcgcc tccttctcta cctttaagtg ctatggcgtg agccccacaa agctgaatga 6120
cctgtgcttt accaacgtgt acgccgattc cttcgtgatc aggggcgacg aggtgcgcca 6180
gatcgcacca ggacagacag gcaatatcgc agactacaat tataagctgc ctgacgattt 6240
caccggctgc gtgatcgcct ggaactctaa caatctggat agcaaagtgg gcggcaacta 6300
caattatctg taccggctgt ttagaaagtc taatctgaag ccattcgaga gggacatctc 6360
cacagaaatc taccaggccg gctctacccc ctgcaatggc gtgaagggct ttaactgtta 6420
tttccctctg cagagctacg gcttccagcc aacatatggc gtgggctatc agccctaccg 6480
cgtggtggtg ctgtcttttg agctgctgca cgcacctgca acagtgtgcg gaccaaagaa 6540
gagcaccaat ctggtgaaga acaagtgcgt gaacttcaac ttcaacggac tgaccggaac 6600
aggcgtgctg accgagtcca acaagaagtt cctgcctttt cagcagttcg gcagggacat 6660
cgcagatacc acagacgccg tgcgcgaccc tcagaccctg gagatcctgg acatcacacc 6720
atgctccttc ggcggcgtgt ctgtgatcac accaggcacc aatacaagca accaggtggc 6780
cgtgctgtat cagggcgtga attgtaccga ggtgccagtg gcaatccacg cagatcagct 6840
gacccctaca tggcgggtgt actctaccgg cagcaacgtg ttccagacaa gagccggatg 6900
cctgatcgga gcagagcacg tgaacaatag ctatgagtgc gacatcccta tcggcgccgg 6960
catctgtgcc tcctaccaga cccagacaaa ctccccagaa tcaagcgtga ttcctctggt 7020
ccatccactg gcagatccct ccacagtgtt caaagacgga gatgaggccg aagactttgt 7080
ggaagtccac ctgcctgatg tgcataacca ggtgtctggc gtcgacctgg gactgccaaa 7140
ttggggcaag tacgtgctgc tgagtgctgg agcactgact gccctgatgc tgatcatttt 7200
cctgatgacc tgctgtcggc gcgtgaacag aagtgagccc actcagcaca atctgcgagg 7260
aaccgggaga gaagtgtcag tcacacctca gagcgggaaa atcattagta gttgggaatc 7320
acataaaagc gggggcgaga ccaggctggg atccggctcc ggcgagggca ggggaagtct 7380
actaacatgc ggggacgtgg aggaaaatcc cggccccatg ctcgatcctg gagaggtcta 7440
tgatgaccct attgacccaa tcgagttaga ggctgaaccc agaggaaccc ccattgtccc 7500
caacatcttg aggaactctg actacaatct caactctcct ttgatagaag atcctgctag 7560
actaatgtta gaatggttaa aaacagggaa tagaccttat cggatgactc taacagacaa 7620
ttgctccagg tctttcagag ttttgaaaga ttatttcaag aaggtagatt tgggttctct 7680
caaggtgggc ggaatggctg cacagtcaat gatttctctc tggttatatg gtgcccactc 7740
tgaatccaac aggagccgga gatgtataac agacttggcc catttctatt ccaagtcgtc 7800
ccccatagag aagctgttga atctcacgct aggaaataga gggctgagaa tccccccaga 7860
gggagtgtta agttgccttg agagggttga ttatgataat gcatttggaa ggtatcttgc 7920
caacacgtat tcctcttact tgttcttcca tgtaatcacc ttatacatga acgccctaga 7980
ctgggatgaa gaaaagacca tcctagcatt atggaaagat ttaacctcag tggacatcgg 8040
gaaggacttg gtaaagttca aagaccaaat atggggactg ctgatcgtga caaaggactt 8100
tgtttactcc caaagttcca attgtctttt tgacagaaac tacacactta tgctaaaaga 8160
tcttttcttg tctcgcttca actccttaat ggtcttgctc tctcccccag agccccgata 8220
ctcagatgac ttgatatctc aactatgcca gctgtacatt gctggggatc aagtcttgtc 8280
tatgtgtgga aactccggct atgaagtcat caaaatattg gagccatatg tcgtgaatag 8340
tttagtccag agagcagaaa agtttaggcc tctcattcat tccttgggag actttcctgt 8400
atttataaaa gacaaggtaa gtcaacttga agagacgttc ggtccctgtg caagaaggtt 8460
ctttagggct ctggatcaat tcgacaacat acatgacttg gtttttgtgt ttggctgtta 8520
caggcattgg gggcacccat atatagatta tcgaaagggt ctgtcaaaac tatatgatca 8580
ggttcacctt aaaaaaatga tagataagtc ctaccaggag tgcttagcaa gcgacctagc 8640
caggaggatc cttagatggg gttttgataa gtactccaag tggtatctgg attcaagatt 8700
cctagcccga gaccacccct tgactcctta tatcaaaacc caaacatggc cacccaaaca 8760
tattgtagac ttggtggggg atacatggca caagctcccg atcacgcaga tctttgagat 8820
tcctgaatca atggatccgt cagaaatatt ggatgacaaa tcacattctt tcaccagaac 8880
gagactagct tcttggctgt cagaaaaccg aggggggcct gttcctagcg aaaaagttat 8940
tatcacggcc ctgtctaagc cgcctgtcaa tccccgagag tttctgaggt ctatagacct 9000
cggaggattg ccagatgaag acttgataat tggcctcaag ccaaaggaac gggaattgaa 9060
gattgaaggt cgattctttg ctctaatgtc atggaatcta agattgtatt ttgtcatcac 9120
tgaaaaactc ttggccaact acatcttgcc actttttgac gcgctgacta tgacagacaa 9180
cctgaacaag gtgtttaaaa agctgatcga cagggtcacc gggcaagggc ttttggacta 9240
ttcaagggtc acatatgcat ttcacctgga ctatgaaaag tggaacaacc atcaaagatt 9300
agagtcaaca gaggatgtat tttctgtcct agatcaagtg tttggattga agagagtgtt 9360
ttctagaaca cacgagtttt ttcaaaaggc ctggatctat tattcagaca gatcagacct 9420
catcgggtta cgggaggatc aaatatactg cttagatgcg tccaacggcc caacctgttg 9480
gaatggccag gatggcgggc tagaaggctt acggcagaag ggctggagtc tagtcagctt 9540
attgatgata gatagagaat ctcaaatcag gaacacaaga accaaaatac tagctcaagg 9600
agacaaccag gttttatgtc cgacatacat gttgtcgcca gggctatctc aagaggggct 9660
cctctatgaa ttggagagaa tatcaaggaa tgcactttcg atatacagag ccgtcgagga 9720
aggggcatct aagctagggc tgatcatcaa gaaagaagag accatgtgta gttatgactt 9780
cctcatctat ggaaaaaccc ctttgtttag aggtaacata ttggtgcctg agtccaaaag 9840
atgggccaga gtctcttgcg tctctaatga ccaaatagtc aacctcgcca atataatgtc 9900
gacagtgtcc accaatgcgc taacagtggc acaacactct caatctttga tcaaaccgat 9960
gagggatttt ctgctcatgt cagtacaggc agtctttcac tacctgctat ttagcccaat 10020
cttaaaggga agagtttaca agattctgag cgctgaaggg gagagctttc tcctagccat 10080
gtcaaggata atctatctag atccttcttt gggagggata tctggaatgt ccctcggaag 10140
attccatata cgacagttct cagaccctgt ctctgaaggg ttatccttct ggagagagat 10200
ctggttaagc tcccaagagt cctggattca cgcgttgtgt caagaggctg gaaacccaga 10260
tcttggagag agaacactcg agagcttcac tcgccttcta gaagatccga ccaccttaaa 10320
tatcagagga ggggccagtc ctaccattct actcaaggat gcaatcagaa aggctttata 10380
tgacgaggtg gacaaggtgg aaaattcaga gtttcgagag gcaatcctgt tgtccaagac 10440
ccatagagat aattttatac tcttcttaat atctgttgag cctctgtttc ctcgatttct 10500
cagtgagcta ttcagttcgt cttttttggg aatccccgag tcaatcattg gattgataca 10560
aaactcccga acgataagaa ggcagtttag aaagagtctc tcaaaaactt tagaagaatc 10620
cttctacaac tcagagatcc acgggattag tcggatgacc cagacacctc agagggttgg 10680
gggggtgtgg ccttgctctt cagagagggc agatctactt agggagatct cttggggaag 10740
aaaagtggta ggcacgacag ttcctcaccc ttctgagatg ttgggattac ttcccaagtc 10800
ctctatttct tgcacttgtg gagcaacagg aggaggcaat cctagagttt ctgtatcagt 10860
actcccgtcc tttgatcagt catttttttc acgaggcccc ctaaagggat acttgggctc 10920
gtccacctct atgtcgaccc agctattcca tgcatgggaa aaagtcacta atgttcatgt 10980
ggtgaagaga gctctatcgt taaaagaatc tataaactgg ttcattacta gagattccaa 11040
cttggctcaa gctctaatta ggaacattat gtctctgaca ggccctgatt tccctctaga 11100
ggaggcccct gtcttcaaaa ggacggggtc agccttgcat aggttcaagt ctgccagata 11160
cagcgaagga gggtattctt ctgtctgccc gaacctcctc tctcatattt ctgttagtac 11220
agacaccatg tctgatttga cccaagacgg gaagaactac gatttcatgt tccagccatt 11280
gatgctttat gcacagacat ggacatcaga gctggtacag agagacacaa ggctaagaga 11340
ctctacgttt cattggcacc tccgatgcaa caggtgtgtg agacccattg acgacgtgac 11400
cctggagacc tctcagatct tcgagtttcc ggatgtgtcg aaaagaatat ccagaatggt 11460
ttctggggct gtgcctcact tccagaggct tcccgatatc cgtctgagac caggagattt 11520
tgaatctcta agcggtagag aaaagtctca ccatatcgga tcagctcagg ggctcttata 11580
ctcaatctta gtggcaattc acgactcagg atacaatgat ggaaccatct tccctgtcaa 11640
catatacggc aaggtttccc ctagagacta tttgagaggg ctcgcaaggg gagtattgat 11700
aggatcctcg atttgcttct tgacaagaat gacaaatatc aatattaata gacctcttga 11760
attggtctca ggggtaatct catatattct cctgaggcta gataaccatc cctccttgta 11820
cataatgctc agagaaccgt ctcttagagg agagatattt tctatccctc agaaaatccc 11880
cgccgcttat ccaaccacta tgaaagaagg caacagatca atcttgtgtt atctccaaca 11940
tgtgctacgc tatgagcgag agataatcac ggcgtctcca gagaatgact ggctatggat 12000
cttttcagac tttagaagtg ccaaaatgac gtacctatcc ctcattactt accagtctca 12060
tcttctactc cagagggttg agagaaacct atctaagagt atgagagata acctgcgaca 12120
attgagttct ttgatgaggc aggtgctggg cgggcacgga gaagatacct tagagtcaga 12180
cgacaacatt caacgactgc taaaagactc tttacgaagg acaagatggg tggatcaaga 12240
ggtgcgccat gcagctagaa ccatgactgg agattacagc cccaacaaga aggtgtcccg 12300
taaggtagga tgttcagaat gggtctgctc tgctcaacag gttgcagtct ctacctcagc 12360
aaacccggcc cctgtctcgg agcttgacat aagggccctc tctaagaggt tccagaaccc 12420
tttgatctcg ggcttgagag tggttcagtg ggcaaccggt gctcattata agcttaagcc 12480
tattctagat gatctcaatg ttttcccatc tctctgcctt gtagttgggg acgggtcagg 12540
ggggatatca agggcagtcc tcaacatgtt tccagatgcc aagcttgtgt tcaacagtct 12600
tttagaggtg aatgacctga tggcttccgg aacacatcca ctgcctcctt cagcaatcat 12660
gaggggagga aatgatatcg tctccagagt gatagatctt gactcaatct gggaaaaacc 12720
gtccgacttg agaaacttgg caacctggaa atacttccag tcagtccaaa agcaggtcaa 12780
catgtcctat gacctcatta tttgcgatgc agaagttact gacattgcat ctatcaaccg 12840
gatcaccctg ttaatgtccg attttgcatt gtctatagat ggaccactct atttggtctt 12900
caaaacttat gggactatgc tagtaaatcc aaactacaag gctattcaac acctgtcaag 12960
agcgttcccc tcggtcacag ggtttatcac ccaagtaact tcgtcttttt catctgagct 13020
ctacctccga ttctccaaac gagggaagtt tttcagagat gctgagtact tgacctcttc 13080
cacccttcga gaaatgagcc ttgtgttatt caattgtagc agccccaaga gtgagatgca 13140
gagagctcgt tccttgaact atcaggatct tgtgagagga tttcctgaag aaatcatatc 13200
aaatccttac aatgagatga tcataactct gattgacagt gatgtagaat cttttctagt 13260
ccacaagatg gttgatgatc ttgagttaca gaggggaact ctgtctaaag tggctatcat 13320
tatagccatc atgatagttt tctccaacag agtcttcaac gtttccaaac ccctaactga 13380
cccctcgttc tatccaccgt ctgatcccaa aatcctgagg cacttcaaca tatgttgcag 13440
tactatgatg tatctatcta ctgctttagg tgacgtccct agcttcgcaa gacttcacga 13500
cctgtataac agacctataa cttattactt cagaaagcaa gtcattcgag ggaacgttta 13560
tctatcttgg agttggtcca acgacacctc agtgttcaaa agggtagcct gtaattctag 13620
cctgagtctg tcatctcact ggatcaggtt gatttacaag atagtgaaga ctaccagact 13680
cgttggcagc atcaaggatc tatccagaga agtggaaaga caccttcata ggtacaacag 13740
gtggatcacc ctagaggata tcagatctag atcatcccta ctagactaca gttgcctgtg 13800
aaccggatac tcctggaagc ctgcccatgc taagactctt gtgtgatgta tcttgaaaaa 13860
aacaagatcc taaatctgaa cctttggttg tttgattgtt tttctcattt ttgttgttta 13920
tttgttaagc gt 13932
<210> 18
<211> 13475
<212> DNA
<213> 人工序列
<220>
<223> VSV载体: Convac V1 China
<400> 18
acgaagacaa acaaaccatt attatcatta aaaggctcag gagaaacttt aacagtaatc 60
aaaatgtctg ttacagtcaa gagaatcatt gacaacacag tcatagttcc aaaacttcct 120
gcaaatgagg atccagtgga atacccggca gattacttca gaaaatcaaa ggagattcct 180
ctttacatca atactacaaa aagtttgtca gatctaagag gatatgtcta ccaaggcctc 240
aaatccggaa atgtatcaat catacatgtc aacagctact tgtatggagc attaaaggac 300
atccggggta agttggataa agattggtca agtttcggaa taaacatcgg gaaagcaggg 360
gatacaatcg gaatatttga ccttgtatcc ttgaaagccc tggacggcgt acttccagat 420
ggagtatcgg atgcttccag aaccagcgca gatgacaaat ggttgccttt gtatctactt 480
ggcttataca gagtgggcag aacacaaatg cctgaataca gaaaaaagct catggatggg 540
ctgacaaatc aatgcaaaat gatcaatgaa cagtttgaac ctcttgtgcc agaaggtcgt 600
gacatttttg atgtgtgggg aaatgacagt aattacacaa aaattgtcgc tgcagtggac 660
atgttcttcc acatgttcaa aaaacatgaa tgtgcctcgt tcagatacgg aactattgtt 720
tccagattca aagattgtgc tgcattggca acatttggac acctctgcaa aataaccgga 780
atgtctacag aagatgtaac gacctggatc ttgaaccgag aagttgcaga tgaaatggtc 840
caaatgatgc ttccaggcca agaaattgac aaggccgatt catacatgcc ttatttgatc 900
gactttggat tgtcttctaa gtctccatat tcttccgtca aaaaccctgc cttccacttc 960
tgggggcaat tgacagctct tctgctcaga tccaccagag caaggaatgc ccgacagcct 1020
gatgacattg agtatacatc tcttactaca gcaggtttgt tgtacgctta tgcagtagga 1080
tcctctgccg acttggcaca acagttttgt gttggagata acaaatacac tccagatgat 1140
agtaccggag gattgacgac taatgcaccg ccacaaggca gagatgtggt cgaatggctc 1200
ggatggtttg aagatcaaaa cagaaaaccg actcctgata tgatgcagta tgcgaaaaga 1260
gcagtcatgt cactgcaagg cctaagagag aagacaattg gcaagtatgc taagtcagaa 1320
tttgacaaat gaccctataa ttctcagatc acctattata tattatgcta catatgaaaa 1380
aaactaacag atatcatgga taatctcaca aaagttcgtg agtatctcaa gtcctattct 1440
cgtctggatc aggcggtagg agagatagat gagatcgaag cacaacgagc tgaaaagtcc 1500
aattatgagt tgttccaaga ggatggagtg gaagagcata ctaagccctc ttattttcag 1560
gcagcagatg attctgacac agaatctgaa ccagaaattg aagacaatca aggtttgtat 1620
gcaccagatc cagaagctga gcaagttgaa ggctttatac aggggccttt agatgactat 1680
gcagatgagg aagtggatgt tgtatttact tcggactgga aacagcctga gcttgaatct 1740
gacgagcatg gaaagacctt acggttgaca tcgccagagg gtttaagtgg agagcagaaa 1800
tcccagtggc tttcgacgat taaagcagtc gtgcaaagtg ccaaatactg gaatctggca 1860
gagtgcacat ttgaagcatc gggagaaggg gtcattatga aggagcgcca gataactccg 1920
gatgtatata aggtcactcc agtgatgaac acacatccgt cccaatcaga agcagtatca 1980
gatgtttggt ctctctcaaa gacatccatg actttccaac ccaagaaagc aagtcttcag 2040
cctctcacca tatccttgga tgaattgttc tcatctagag gagagttcat ctctgtcgga 2100
ggtgacggac gaatgtctca taaagaggcc atcctgctcg gcctgagata caaaaagttg 2160
tacaatcagg cgagagtcaa atattctctg tagactatga aaaaaagtaa cagatatcac 2220
gatctaagtg ttatcccaat ccattcatca tgagttcctt aaagaagatt ctcggtctga 2280
aggggaaagg taagaaatct aagaaattag ggatcgcacc acccccttat gaagaggaca 2340
ctagcatgga gtatgctccg agcgctccaa ttgacaaatc ctattttgga gttgacgaga 2400
tggacaccta tgatccgaat caattaagat atgagaaatt cttctttaca gtgaaaatga 2460
cggttagatc taatcgtccg ttcagaacat actcagatgt ggcagccgct gtatcccatt 2520
gggatcacat gtacatcgga atggcaggga aacgtccctt ctacaaaatc ttggcttttt 2580
tgggttcttc taatctaaag gccactccag cggtattggc agatcaaggt caaccagagt 2640
atcacactca ctgcgaaggc agggcttatt tgccacatag gatggggaag acccctccca 2700
tgctcaatgt accagagcac ttcagaagac cattcaatat aggtctttac aagggaacga 2760
ttgagctcac aatgaccatc tacgatgatg agtcactgga agcagctcct atgatctggg 2820
atcatttcaa ttcttccaaa ttttctgatt tcagagagaa ggccttaatg tttggcctga 2880
ttgtcgagaa aaaggcatct ggagcgtggg tcctggattc tatcagccac ttcaaatgag 2940
ctagtctaac ttctagcttc tgaacaatcc ccggtttact cagtctctcc taattccagc 3000
ctctcgaaca actaatatcc tgtcttttct atccctatga aaaaaactaa cagagatcga 3060
tctgtttacg cgtcactatg aagtgccttt tgtacttagc ctttttattc attggggtga 3120
attgcaagtt caccatagtt tttccacaca accaaaaagg aaactggaaa aatgttcctt 3180
ctaattacca ttattgcccg tcaagctcag atttaaattg gcataatgac ttaataggca 3240
cagccataca agtcaaaatg cccaagagtc acaaggctat tcaagcagac ggttggatgt 3300
gtcatgcttc caaatgggtc actacttgtg atttccgctg gtatggaccg aagtatataa 3360
cacagtccat ccgatccttc actccatctg tagaacaatg caaggaaagc attgaacaaa 3420
cgaaacaagg aacttggctg aatccaggct tccctcctca aagttgtgga tatgcaactg 3480
tgacggatgc cgaagcagtg attgtccagg tgactcctca ccatgtgctg gttgatgaat 3540
acacaggaga atgggttgat tcacagttca tcaacggaaa atgcagcaat tacatatgcc 3600
ccactgtcca taactctaca acctggcatt ctgactataa ggtcaaaggg ctatgtgatt 3660
ctaacctcat ttccatggac atcaccttct tctcagagga cggagagcta tcatccctgg 3720
gaaaggaggg cacagggttc agaagtaact actttgctta tgaaactgga ggcaaggcct 3780
gcaaaatgca atactgcaag cattggggag tcagactccc atcaggtgtc tggttcgaga 3840
tggctgataa ggatctcttt gctgcagcca gattccctga atgcccagaa gggtcaagta 3900
tctctgctcc atctcagacc tcagtggatg taagtctaat tcaggacgtt gagaggatct 3960
tggattattc cctctgccaa gaaacctgga gcaaaatcag agcgggtctt ccaatctctc 4020
cagtggatct cagctatctt gctcctaaaa acccaggaac cggtcctgct ttcaccataa 4080
tcaatggtac cctaaaatac tttgagacca gatacatcag agtcgatatt gctgctccaa 4140
tcctctcaag aatggtcgga atgatcagtg gaactaccac agaaagggaa ctgtgggatg 4200
actgggcacc atatgaagac gtggaaattg gacccaatgg agttctgagg accagttcag 4260
gatataagtt tcctttatac atgattggac atggtatgtt ggactccgat cttcatctta 4320
gctcaaaggc tcaggtgttc gaacatcctc acattcaaga cgctgcttcg caacttcctg 4380
atgatgagag tttatttttt ggtgatactg ggctatccaa aaatccaatc gagcttgtag 4440
aaggttggtt cagtagttgg aaaagctcta ttgcctcttt tttctttatc atagggttaa 4500
tcattggact attcttggtt ctccgagttg gtatccatct ttgcattaaa ttaaagcaca 4560
ccaagaaaag acagatttat acagacatag agatgaaccg acttggaaag taactcaaat 4620
cctgctaggt atgaaaaaaa ctaacagata tcacgctcga gcgtacgcca ccatgttcgt 4680
gtttctggtg ctgctgcctc tggtgagctc ccagtgcgtg aacctgacca caaggaccca 4740
gctgccccct gcctatacca attccttcac acggggcgtg tactatcccg acaaggtgtt 4800
ccggagcagc gtgctgcact ccacacagga tctgtttctg cctttctttt ctaacgtgac 4860
ctggttccac gccatccacg tgagcggcac caatggcaca aagcggttcg acaatccagt 4920
gctgcccttt aacgatggcg tgtacttcgc ctccaccgag aagtctaaca tcatcagagg 4980
ctggatcttt ggcaccacac tggacagcaa gacacagtcc ctgctgatcg tgaacaatgc 5040
caccaacgtg gtcatcaagg tgtgcgagtt ccagttttgt aatgatccat tcctgggcgt 5100
gtactatcac aagaacaata agtcttggat ggagagcgag tttcgcgtgt attcctctgc 5160
caacaattgc acatttgagt acgtgtccca gcccttcctg atggacctgg agggcaagca 5220
gggcaatttc aagaacctga gggagttcgt gtttaagaat atcgatggct acttcaaaat 5280
ctactccaag cacaccccaa tcaacctggt gcgcgacctg ccacagggct tctctgccct 5340
ggagccactg gtggatctgc ccatcggcat caacatcacc cggtttcaga cactgctggc 5400
cctgcacaga agctacctga caccaggcga cagctcctct ggatggaccg caggagcagc 5460
agcctactat gtgggctatc tgcagcccag gaccttcctg ctgaagtaca acgagaatgg 5520
caccatcaca gacgccgtgg attgcgccct ggatcccctg tctgagacca agtgtacact 5580
gaagagcttt accgtggaga agggcatcta tcagacaagc aatttcaggg tgcagcctac 5640
cgagtccatc gtgcgctttc ccaatatcac aaacctgtgc ccttttggcg aggtgttcaa 5700
cgcaacccgc ttcgccagcg tgtacgcctg gaataggaag cgcatctcca actgcgtggc 5760
cgactattct gtgctgtaca acagcgcctc cttctctacc tttaagtgct atggcgtgag 5820
ccccacaaag ctgaatgacc tgtgctttac caacgtgtac gccgattcct tcgtgatcag 5880
gggcgacgag gtgcgccaga tcgcaccagg acagacaggc aagatcgcag actacaatta 5940
taagctgcct gacgatttca ccggctgcgt gatcgcctgg aactctaaca atctggatag 6000
caaagtgggc ggcaactaca attatctgta ccggctgttt agaaagtcta atctgaagcc 6060
attcgagagg gacatctcca cagaaatcta ccaggccggc tctaccccct gcaatggcgt 6120
ggagggcttt aactgttatt tccctctgca gagctacggc ttccagccaa caaacggcgt 6180
gggctatcag ccctaccgcg tggtggtgct gtcttttgag ctgctgcacg cacctgcaac 6240
agtgtgcgga ccaaagaaga gcaccaatct ggtgaagaac aagtgcgtga acttcaactt 6300
caacggactg accggaacag gcgtgctgac cgagtccaac aagaagttcc tgccttttca 6360
gcagttcggc agggacatcg cagataccac agacgccgtg cgcgaccctc agaccctgga 6420
gatcctggac atcacaccat gctccttcgg cggcgtgtct gtgatcacac caggcaccaa 6480
tacaagcaac caggtggccg tgctgtatca ggacgtgaat tgtaccgagg tgccagtggc 6540
aatccacgca gatcagctga cccctacatg gcgggtgtac tctaccggca gcaacgtgtt 6600
ccagacaaga gccggatgcc tgatcggagc agagcacgtg aacaatagct atgagtgcga 6660
catccctatc ggcgccggca tctgtgcctc ctaccagacc cagacaaact ccccaaggtc 6720
tgtgggcgat acaggcctgt ccaagaatcc aatcgagctg gtagagggct ggttcagcag 6780
ttggaaaagc tccatcgcct cctttttctt tatcatcggc ctgatcatcg gactgttcct 6840
ggtgctccgc gtgggtatcc acctgtgcat caagctgaag cacaccaaga aaagacagat 6900
ttatacagac atcgagatga accgcctggg aaagtgagct agccagattc ttcatgtttg 6960
gaccaaatca acttgtgata ccatgctcaa agaggcctca attatatttg agtttttaat 7020
ttttatgaaa aaaactaaca gcaatcatgg aagtccacga ttttgagacc gacgagttca 7080
atgatttcaa tgaagatgac tatgccacaa gagaattcct gaatcccgat gagcgcatga 7140
cgtacttgaa tcatgctgat tacaatttga attctcctct aattagtgat gatattgaca 7200
atttgatcag gaaattcaat tctcttccga ttccctcgat gtgggatagt aagaactggg 7260
atggagttct tgagatgtta acatcatgtc aagccaatcc catctcaaca tctcagatgc 7320
ataaatggat gggaagttgg ttaatgtctg ataatcatga tgccagtcaa gggtatagtt 7380
ttttacatga agtggacaaa gaggcagaaa taacatttga cgtggtggag accttcatcc 7440
gcggctgggg caacaaacca attgaataca tcaaaaagga aagatggact gactcattca 7500
aaattctcgc ttatttgtgt caaaagtttt tggacttaca caagttgaca ttaatcttaa 7560
atgctgtctc tgaggtggaa ttgctcaact tggcgaggac tttcaaaggc aaagtcagaa 7620
gaagttctca tggaacgaac atatgcagga ttagggttcc cagcttgggt cctactttta 7680
tttcagaagg atgggcttac ttcaagaaac ttgatattct aatggaccga aactttctgt 7740
taatggtcaa agatgtgatt atagggagga tgcaaacggt gctatccatg gtatgtagaa 7800
tagacaacct gttctcagag caagacatct tctcccttct aaatatctac agaattggag 7860
ataaaattgt ggagaggcag ggaaattttt cttatgactt gattaaaatg gtggaaccga 7920
tatgcaactt gaagctgatg aaattagcaa gagaatcaag gcctttagtc ccacaattcc 7980
ctcattttga aaatcatatc aagacttctg ttgatgaagg ggcaaaaatt gaccgaggta 8040
taagattcct ccatgatcag ataatgagtg tgaaaacagt ggatctcaca ctggtgattt 8100
atggatcgtt cagacattgg ggtcatcctt ttatagatta ttacactgga ctagaaaaat 8160
tacattccca agtaaccatg aagaaagata ttgatgtgtc atatgcaaaa gcacttgcaa 8220
gtgatttagc tcggattgtt ctatttcaac agttcaatga tcataaaaag tggttcgtga 8280
atggagactt gctccctcat gatcatccct ttaaaagtca tgttaaagaa aatacatggc 8340
ccacagctgc tcaagttcaa gattttggag ataaatggca tgaacttccg ctgattaaat 8400
gttttgaaat acccgactta ctagacccat cgataatata ctctgacaaa agtcattcaa 8460
tgaataggtc agaggtgttg aaacatgtcc gaatgaatcc gaacactcct atccctagta 8520
aaaaggtgtt gcagactatg ttggacacaa aggctaccaa ttggaaagaa tttcttaaag 8580
agattgatga gaagggctta gatgatgatg atctaattat tggtcttaaa ggaaaggaga 8640
gggaactgaa gttggcaggt agatttttct ccctaatgtc ttggaaattg cgagaatact 8700
ttgtaattac cgaatatttg ataaagactc atttcgtccc tatgtttaaa ggcctgacaa 8760
tggcggacga tctaactgca gtcattaaaa agatgttaga ttcctcatcc ggccaaggat 8820
tgaagtcata tgaggcaatt tgcatagcca atcacattga ttacgaaaaa tggaataacc 8880
accaaaggaa gttatcaaac ggcccagtgt tccgagttat gggccagttc ttaggttatc 8940
catccttaat cgagagaact catgaatttt ttgagaaaag tcttatatac tacaatggaa 9000
gaccagactt gatgcgtgtt cacaacaaca cactgatcaa ttcaacctcc caacgagttt 9060
gttggcaagg acaagagggt ggactggaag gtctacggca aaaaggatgg actatcctca 9120
atctactggt tattcaaaga gaggctaaaa tcagaaacac tgctgtcaaa gtcttggcac 9180
aaggtgataa tcaagttatt tgcacacagt ataaaacgaa gaaatcgaga aacgttgtag 9240
aattacaggg tgctctcaat caaatggttt ctaataatga gaaaattatg actgcaatca 9300
aaatagggac agggaagtta ggacttttga taaatgacga tgagactatg caatctgcag 9360
attacttgaa ttatggaaaa ataccgattt tccgtggagt gattagaggg ttagagacca 9420
agagatggtc acgagtgact tgtgtcacca atgaccaaat acccacttgt gctaatataa 9480
tgagctcagt ttccacaaat gctctcaccg tagctcattt tgctgagaac ccaatcaatg 9540
ccatgataca gtacaattat tttgggacat ttgctagact cttgttgatg atgcatgatc 9600
ctgctcttcg tcaatcattg tatgaagttc aagataagat accgggcttg cacagttcta 9660
ctttcaaata cgccatgttg tatttggacc cttccattgg aggagtgtcg ggcatgtctt 9720
tgtccaggtt tttgattaga gccttcccag atcccgtaac agaaagtctc tcattctgga 9780
gattcatcca tgtacatgct cgaagtgagc atctgaagga gatgagtgca gtatttggaa 9840
accccgagat agccaagttt cgaataactc acatagacaa gctagtagaa gatccaacct 9900
ctctgaacat cgctatggga atgagtccag cgaacttgtt aaagactgag gttaaaaaat 9960
gcttaatcga atcaagacaa accatcagga accaggtgat taaggatgca accatatatt 10020
tgtatcatga agaggatcgg ctcagaagtt tcttatggtc aataaatcct ctgttcccta 10080
gatttttaag tgaattcaaa tcaggcactt ttttgggagt cgcagacggg ctcatcagtc 10140
tatttcaaaa ttctcgtact attcggaact cctttaagaa aaagtatcat agggaattgg 10200
atgatttgat tgtgaggagt gaggtatcct ctttgacaca tttagggaaa cttcatttga 10260
gaaggggatc atgtaaaatg tggacatgtt cagctactca tgctgacaca ttaagataca 10320
aatcctgggg ccgtacagtt attgggacaa ctgtacccca tccattagaa atgttgggtc 10380
cacaacatcg aaaagagact ccttgtgcac catgtaacac atcagggttc aattatgttt 10440
ctgtgcattg tccagacggg atccatgacg tctttagttc acggggacca ttgcctgctt 10500
atctagggtc taaaacatct gaatctacat ctattttgca gccttgggaa agggaaagca 10560
aagtcccact gattaaaaga gctacacgtc ttagagatgc tatctcttgg tttgttgaac 10620
ccgactctaa actagcaatg actatacttt ctaacatcca ctctttaaca ggcgaagaat 10680
ggaccaaaag gcagcatggg ttcaaaagaa cagggtctgc ccttcatagg ttttcgacat 10740
ctcggatgag ccatggtggg ttcgcatctc agagcactgc agcattgacc aggttgatgg 10800
caactacaga caccatgagg gatctgggag atcagaattt cgacttttta ttccaagcaa 10860
cgttgctcta tgctcaaatt accaccactg ttgcaagaga cggatggatc accagttgta 10920
cagatcatta tcatattgcc tgtaagtcct gtttgagacc catagaagag atcaccctgg 10980
actcaagtat ggactacacg cccccagatg tatcccatgt gctgaagaca tggaggaatg 11040
gggaaggttc gtggggacaa gagataaaac agatctatcc tttagaaggg aattggaaga 11100
atttagcacc tgctgagcaa tcctatcaag tcggcagatg tataggtttt ctatatggag 11160
acttggcgta tagaaaatct actcatgccg aggacagttc tctatttcct ctatctatac 11220
aaggtcgtat tagaggtcga ggtttcttaa aagggttgct agacggatta atgagagcaa 11280
gttgctgcca agtaatacac cggagaagtc tggctcattt gaagaggccg gccaacgcag 11340
tgtacggagg tttgatttac ttgattgata aattgagtgt atcacctcca ttcctttctc 11400
ttactagatc aggacctatt agagacgaat tagaaacgat tccccacaag atcccaacct 11460
cctatccgac aagcaaccgt gatatggggg tgattgtcag aaattacttc aaataccaat 11520
gccgtctaat tgaaaaggga aaatacagat cacattattc acaattatgg ttattctcag 11580
atgtcttatc catagacttc attggaccat tctctatttc caccaccctc ttgcaaatcc 11640
tatacaagcc atttttatct gggaaagata agaatgagtt gagagagctg gcaaatcttt 11700
cttcattgct aagatcagga gaggggtggg aagacataca tgtgaaattc ttcaccaagg 11760
acatattatt gtgtccagag gaaatcagac atgcttgcaa gttcgggatt gctaaggata 11820
ataataaaga catgagctat cccccttggg gaagggaatc cagagggaca attacaacaa 11880
tccctgttta ttatacgacc accccttacc caaagatgct agagatgcct ccaagaatcc 11940
aaaatcccct gctgtccgga atcaggttgg gccaattacc aactggcgct cattataaaa 12000
ttcggagtat attacatgga atgggaatcc attacaggga cttcttgagt tgtggagacg 12060
gctccggagg gatgactgct gcattactac gagaaaatgt gcatagcaga ggaatattca 12120
atagtctgtt agaattatca gggtcagtca tgcgaggcgc ctctcctgag ccccccagtg 12180
ccctagaaac tttaggagga gataaatcga gatgtgtaaa tggtgaaaca tgttgggaat 12240
atccatctga cttatgtgac ccaaggactt gggactattt cctccgactc aaagcaggct 12300
tggggcttca aattgattta attgtaatgg atatggaagt tcgggattct tctactagcc 12360
tgaaaattga gacgaatgtt agaaattatg tgcaccggat tttggatgag caaggagttt 12420
taatctacaa gacttatgga acatatattt gtgagagcga aaagaatgca gtaacaatcc 12480
ttggtcccat gttcaagacg gtcgacttag ttcaaacaga atttagtagt tctcaaacgt 12540
ctgaagtata tatggtatgt aaaggtttga agaaattaat cgatgaaccc aatcccgatt 12600
ggtcttccat caatgaatcc tggaaaaacc tgtacgcatt ccagtcatca gaacaggaat 12660
ttgccagagc aaagaaggtt agtacatact ttaccttgac aggtattccc tcccaattca 12720
ttcctgatcc ttttgtaaac attgagacta tgctacaaat attcggagta cccacgggtg 12780
tgtctcatgc ggctgcctta aaatcatctg atagacctgc agatttattg accattagcc 12840
ttttttatat ggcgattata tcgtattata acatcaatca tatcagagta ggaccgatac 12900
ctccgaaccc cccatcagat ggaattgcac aaaatgtggg gatcgctata actggtataa 12960
gcttttggct gagtttgatg gagaaagaca ttccactata tcaacagtgt ttagcagtta 13020
tccagcaatc attcccgatt aggtgggagg ctgtttcagt aaaaggagga tacaagcaga 13080
agtggagtac tagaggtgat gggctcccaa aagatacccg aacttcagac tccttggccc 13140
caatcgggaa ctggatcaga tctctggaat tggtccgaaa ccaagttcgt ctaaatccat 13200
tcaatgagat cttgttcaat cagctatgtc gtacagtgga taatcatttg aaatggtcaa 13260
atttgcgaag aaacacagga atgattgaat ggatcaatag acgaatttca aaagaagacc 13320
ggtctatact gatgttgaag agtgacctac acgaggaaaa ctcttggaga gattaaaaaa 13380
tcatgaggag actccaaact ttaagtatga aaaaaacttt gatccttaag accctcttgt 13440
ggtttttatt ttttatctgg ttttgtggtc ttcgt 13475
<210> 19
<211> 13469
<212> DNA
<213> 人工序列
<220>
<223> VSV载体: Convac V1 South Africa
<400> 19
acgaagacaa acaaaccatt attatcatta aaaggctcag gagaaacttt aacagtaatc 60
aaaatgtctg ttacagtcaa gagaatcatt gacaacacag tcatagttcc aaaacttcct 120
gcaaatgagg atccagtgga atacccggca gattacttca gaaaatcaaa ggagattcct 180
ctttacatca atactacaaa aagtttgtca gatctaagag gatatgtcta ccaaggcctc 240
aaatccggaa atgtatcaat catacatgtc aacagctact tgtatggagc attaaaggac 300
atccggggta agttggataa agattggtca agtttcggaa taaacatcgg gaaagcaggg 360
gatacaatcg gaatatttga ccttgtatcc ttgaaagccc tggacggcgt acttccagat 420
ggagtatcgg atgcttccag aaccagcgca gatgacaaat ggttgccttt gtatctactt 480
ggcttataca gagtgggcag aacacaaatg cctgaataca gaaaaaagct catggatggg 540
ctgacaaatc aatgcaaaat gatcaatgaa cagtttgaac ctcttgtgcc agaaggtcgt 600
gacatttttg atgtgtgggg aaatgacagt aattacacaa aaattgtcgc tgcagtggac 660
atgttcttcc acatgttcaa aaaacatgaa tgtgcctcgt tcagatacgg aactattgtt 720
tccagattca aagattgtgc tgcattggca acatttggac acctctgcaa aataaccgga 780
atgtctacag aagatgtaac gacctggatc ttgaaccgag aagttgcaga tgaaatggtc 840
caaatgatgc ttccaggcca agaaattgac aaggccgatt catacatgcc ttatttgatc 900
gactttggat tgtcttctaa gtctccatat tcttccgtca aaaaccctgc cttccacttc 960
tgggggcaat tgacagctct tctgctcaga tccaccagag caaggaatgc ccgacagcct 1020
gatgacattg agtatacatc tcttactaca gcaggtttgt tgtacgctta tgcagtagga 1080
tcctctgccg acttggcaca acagttttgt gttggagata acaaatacac tccagatgat 1140
agtaccggag gattgacgac taatgcaccg ccacaaggca gagatgtggt cgaatggctc 1200
ggatggtttg aagatcaaaa cagaaaaccg actcctgata tgatgcagta tgcgaaaaga 1260
gcagtcatgt cactgcaagg cctaagagag aagacaattg gcaagtatgc taagtcagaa 1320
tttgacaaat gaccctataa ttctcagatc acctattata tattatgcta catatgaaaa 1380
aaactaacag atatcatgga taatctcaca aaagttcgtg agtatctcaa gtcctattct 1440
cgtctggatc aggcggtagg agagatagat gagatcgaag cacaacgagc tgaaaagtcc 1500
aattatgagt tgttccaaga ggatggagtg gaagagcata ctaagccctc ttattttcag 1560
gcagcagatg attctgacac agaatctgaa ccagaaattg aagacaatca aggtttgtat 1620
gcaccagatc cagaagctga gcaagttgaa ggctttatac aggggccttt agatgactat 1680
gcagatgagg aagtggatgt tgtatttact tcggactgga aacagcctga gcttgaatct 1740
gacgagcatg gaaagacctt acggttgaca tcgccagagg gtttaagtgg agagcagaaa 1800
tcccagtggc tttcgacgat taaagcagtc gtgcaaagtg ccaaatactg gaatctggca 1860
gagtgcacat ttgaagcatc gggagaaggg gtcattatga aggagcgcca gataactccg 1920
gatgtatata aggtcactcc agtgatgaac acacatccgt cccaatcaga agcagtatca 1980
gatgtttggt ctctctcaaa gacatccatg actttccaac ccaagaaagc aagtcttcag 2040
cctctcacca tatccttgga tgaattgttc tcatctagag gagagttcat ctctgtcgga 2100
ggtgacggac gaatgtctca taaagaggcc atcctgctcg gcctgagata caaaaagttg 2160
tacaatcagg cgagagtcaa atattctctg tagactatga aaaaaagtaa cagatatcac 2220
gatctaagtg ttatcccaat ccattcatca tgagttcctt aaagaagatt ctcggtctga 2280
aggggaaagg taagaaatct aagaaattag ggatcgcacc acccccttat gaagaggaca 2340
ctagcatgga gtatgctccg agcgctccaa ttgacaaatc ctattttgga gttgacgaga 2400
tggacaccta tgatccgaat caattaagat atgagaaatt cttctttaca gtgaaaatga 2460
cggttagatc taatcgtccg ttcagaacat actcagatgt ggcagccgct gtatcccatt 2520
gggatcacat gtacatcgga atggcaggga aacgtccctt ctacaaaatc ttggcttttt 2580
tgggttcttc taatctaaag gccactccag cggtattggc agatcaaggt caaccagagt 2640
atcacactca ctgcgaaggc agggcttatt tgccacatag gatggggaag acccctccca 2700
tgctcaatgt accagagcac ttcagaagac cattcaatat aggtctttac aagggaacga 2760
ttgagctcac aatgaccatc tacgatgatg agtcactgga agcagctcct atgatctggg 2820
atcatttcaa ttcttccaaa ttttctgatt tcagagagaa ggccttaatg tttggcctga 2880
ttgtcgagaa aaaggcatct ggagcgtggg tcctggattc tatcagccac ttcaaatgag 2940
ctagtctaac ttctagcttc tgaacaatcc ccggtttact cagtctctcc taattccagc 3000
ctctcgaaca actaatatcc tgtcttttct atccctatga aaaaaactaa cagagatcga 3060
tctgtttacg cgtcactatg aagtgccttt tgtacttagc ctttttattc attggggtga 3120
attgcaagtt caccatagtt tttccacaca accaaaaagg aaactggaaa aatgttcctt 3180
ctaattacca ttattgcccg tcaagctcag atttaaattg gcataatgac ttaataggca 3240
cagccataca agtcaaaatg cccaagagtc acaaggctat tcaagcagac ggttggatgt 3300
gtcatgcttc caaatgggtc actacttgtg atttccgctg gtatggaccg aagtatataa 3360
cacagtccat ccgatccttc actccatctg tagaacaatg caaggaaagc attgaacaaa 3420
cgaaacaagg aacttggctg aatccaggct tccctcctca aagttgtgga tatgcaactg 3480
tgacggatgc cgaagcagtg attgtccagg tgactcctca ccatgtgctg gttgatgaat 3540
acacaggaga atgggttgat tcacagttca tcaacggaaa atgcagcaat tacatatgcc 3600
ccactgtcca taactctaca acctggcatt ctgactataa ggtcaaaggg ctatgtgatt 3660
ctaacctcat ttccatggac atcaccttct tctcagagga cggagagcta tcatccctgg 3720
gaaaggaggg cacagggttc agaagtaact actttgctta tgaaactgga ggcaaggcct 3780
gcaaaatgca atactgcaag cattggggag tcagactccc atcaggtgtc tggttcgaga 3840
tggctgataa ggatctcttt gctgcagcca gattccctga atgcccagaa gggtcaagta 3900
tctctgctcc atctcagacc tcagtggatg taagtctaat tcaggacgtt gagaggatct 3960
tggattattc cctctgccaa gaaacctgga gcaaaatcag agcgggtctt ccaatctctc 4020
cagtggatct cagctatctt gctcctaaaa acccaggaac cggtcctgct ttcaccataa 4080
tcaatggtac cctaaaatac tttgagacca gatacatcag agtcgatatt gctgctccaa 4140
tcctctcaag aatggtcgga atgatcagtg gaactaccac agaaagggaa ctgtgggatg 4200
actgggcacc atatgaagac gtggaaattg gacccaatgg agttctgagg accagttcag 4260
gatataagtt tcctttatac atgattggac atggtatgtt ggactccgat cttcatctta 4320
gctcaaaggc tcaggtgttc gaacatcctc acattcaaga cgctgcttcg caacttcctg 4380
atgatgagag tttatttttt ggtgatactg ggctatccaa aaatccaatc gagcttgtag 4440
aaggttggtt cagtagttgg aaaagctcta ttgcctcttt tttctttatc atagggttaa 4500
tcattggact attcttggtt ctccgagttg gtatccatct ttgcattaaa ttaaagcaca 4560
ccaagaaaag acagatttat acagacatag agatgaaccg acttggaaag taactcaaat 4620
cctgctaggt atgaaaaaaa ctaacagata tcacgctcga gccaccatgt tcgtgtttct 4680
ggtgctgctg cctctggtga gctcccagtg cgtgaacttc accacaagga cccagctgcc 4740
ccctgcctat accaattcct tcacacgggg cgtgtactat cccgacaagg tgttccggag 4800
cagcgtgctg cactccacac aggatctgtt tctgcctttc ttttctaacg tgacctggtt 4860
ccacgccatc cacgtgagcg gcaccaatgg cacaaagcgg ttcgccaatc cagtgctgcc 4920
ctttaacgat ggcgtgtact tcgcctccac cgagaagtct aacatcatca gaggctggat 4980
ctttggcacc acactggaca gcaagacaca gtccctgctg atcgtgaaca atgccaccaa 5040
cgtggtcatc aaggtgtgcg agttccagtt ttgtaatgat ccattcctgg gcgtgtacta 5100
tcacaagaac aataagtctt ggatggagag cgagtttcgc gtgtattcct ctgccaacaa 5160
ttgcacattt gagtacgtgt cccagccctt cctgatggac ctggagggca agcagggcaa 5220
tttcaagaac ctgagggagt tcgtgtttaa gaatatcgat ggctacttca aaatctactc 5280
caagcacacc ccaatcaacc tggtgcgcgg cctgccacag ggcttctctg ccctggagcc 5340
actggtggat ctgcccatcg gcatcaacat cacccggttt cagacactgc tggccctgca 5400
cagaagctac ctgacaccag gcgacagctc ctctggatgg accgcaggag cagcagccta 5460
ctatgtgggc tatctgcagc ccaggacctt cctgctgaag tacaacgaga atggcaccat 5520
cacagacgcc gtggattgcg ccctggatcc cctgtctgag accaagtgta cactgaagag 5580
ctttaccgtg gagaagggca tctatcagac aagcaatttc agggtgcagc ctaccgagtc 5640
catcgtgcgc tttcccaata tcacaaacct gtgccctttt ggcgaggtgt tcaacgcaac 5700
ccgcttcgcc agcgtgtacg cctggaatag gaagcgcatc tccaactgcg tggccgacta 5760
ttctgtgctg tacaacagcg cctccttctc tacctttaag tgctatggcg tgagccccac 5820
aaagctgaat gacctgtgct ttaccaacgt gtacgccgat tccttcgtga tcaggggcga 5880
cgaggtgcgc cagatcgcac caggacagac aggcaatatc gcagactaca attataagct 5940
gcctgacgat ttcaccggct gcgtgatcgc ctggaactct aacaatctgg atagcaaagt 6000
gggcggcaac tacaattatc tgtaccggct gtttagaaag tctaatctga agccattcga 6060
gagggacatc tccacagaaa tctaccaggc cggctctacc ccctgcaatg gcgtgaaggg 6120
ctttaactgt tatttccctc tgcagagcta cggcttccag ccaacatatg gcgtgggcta 6180
tcagccctac cgcgtggtgg tgctgtcttt tgagctgctg cacgcacctg caacagtgtg 6240
cggaccaaag aagagcacca atctggtgaa gaacaagtgc gtgaacttca acttcaacgg 6300
actgaccgga acaggcgtgc tgaccgagtc caacaagaag ttcctgcctt ttcagcagtt 6360
cggcagggac atcgcagata ccacagacgc cgtgcgcgac cctcagaccc tggagatcct 6420
ggacatcaca ccatgctcct tcggcggcgt gtctgtgatc acaccaggca ccaatacaag 6480
caaccaggtg gccgtgctgt atcagggcgt gaattgtacc gaggtgccag tggcaatcca 6540
cgcagatcag ctgaccccta catggcgggt gtactctacc ggcagcaacg tgttccagac 6600
aagagccgga tgcctgatcg gagcagagca cgtgaacaat agctatgagt gcgacatccc 6660
tatcggcgcc ggcatctgtg cctcctacca gacccagaca aactccccaa ggtctgtggg 6720
cgatacaggc ctgtccaaga atccaatcga gctggtagag ggctggttca gcagttggaa 6780
aagctccatc gcctcctttt tctttatcat cggcctgatc atcggactgt tcctggtgct 6840
ccgcgtgggt atccacctgt gcatcaagct gaagcacacc aagaaaagac agatttatac 6900
agacatcgag atgaaccgcc tgggaaagtg agctagccag attcttcatg tttggaccaa 6960
atcaacttgt gataccatgc tcaaagaggc ctcaattata tttgagtttt taatttttat 7020
gaaaaaaact aacagcaatc atggaagtcc acgattttga gaccgacgag ttcaatgatt 7080
tcaatgaaga tgactatgcc acaagagaat tcctgaatcc cgatgagcgc atgacgtact 7140
tgaatcatgc tgattacaat ttgaattctc ctctaattag tgatgatatt gacaatttga 7200
tcaggaaatt caattctctt ccgattccct cgatgtggga tagtaagaac tgggatggag 7260
ttcttgagat gttaacatca tgtcaagcca atcccatctc aacatctcag atgcataaat 7320
ggatgggaag ttggttaatg tctgataatc atgatgccag tcaagggtat agttttttac 7380
atgaagtgga caaagaggca gaaataacat ttgacgtggt ggagaccttc atccgcggct 7440
ggggcaacaa accaattgaa tacatcaaaa aggaaagatg gactgactca ttcaaaattc 7500
tcgcttattt gtgtcaaaag tttttggact tacacaagtt gacattaatc ttaaatgctg 7560
tctctgaggt ggaattgctc aacttggcga ggactttcaa aggcaaagtc agaagaagtt 7620
ctcatggaac gaacatatgc aggattaggg ttcccagctt gggtcctact tttatttcag 7680
aaggatgggc ttacttcaag aaacttgata ttctaatgga ccgaaacttt ctgttaatgg 7740
tcaaagatgt gattataggg aggatgcaaa cggtgctatc catggtatgt agaatagaca 7800
acctgttctc agagcaagac atcttctccc ttctaaatat ctacagaatt ggagataaaa 7860
ttgtggagag gcagggaaat ttttcttatg acttgattaa aatggtggaa ccgatatgca 7920
acttgaagct gatgaaatta gcaagagaat caaggccttt agtcccacaa ttccctcatt 7980
ttgaaaatca tatcaagact tctgttgatg aaggggcaaa aattgaccga ggtataagat 8040
tcctccatga tcagataatg agtgtgaaaa cagtggatct cacactggtg atttatggat 8100
cgttcagaca ttggggtcat ccttttatag attattacac tggactagaa aaattacatt 8160
cccaagtaac catgaagaaa gatattgatg tgtcatatgc aaaagcactt gcaagtgatt 8220
tagctcggat tgttctattt caacagttca atgatcataa aaagtggttc gtgaatggag 8280
acttgctccc tcatgatcat ccctttaaaa gtcatgttaa agaaaataca tggcccacag 8340
ctgctcaagt tcaagatttt ggagataaat ggcatgaact tccgctgatt aaatgttttg 8400
aaatacccga cttactagac ccatcgataa tatactctga caaaagtcat tcaatgaata 8460
ggtcagaggt gttgaaacat gtccgaatga atccgaacac tcctatccct agtaaaaagg 8520
tgttgcagac tatgttggac acaaaggcta ccaattggaa agaatttctt aaagagattg 8580
atgagaaggg cttagatgat gatgatctaa ttattggtct taaaggaaag gagagggaac 8640
tgaagttggc aggtagattt ttctccctaa tgtcttggaa attgcgagaa tactttgtaa 8700
ttaccgaata tttgataaag actcatttcg tccctatgtt taaaggcctg acaatggcgg 8760
acgatctaac tgcagtcatt aaaaagatgt tagattcctc atccggccaa ggattgaagt 8820
catatgaggc aatttgcata gccaatcaca ttgattacga aaaatggaat aaccaccaaa 8880
ggaagttatc aaacggccca gtgttccgag ttatgggcca gttcttaggt tatccatcct 8940
taatcgagag aactcatgaa ttttttgaga aaagtcttat atactacaat ggaagaccag 9000
acttgatgcg tgttcacaac aacacactga tcaattcaac ctcccaacga gtttgttggc 9060
aaggacaaga gggtggactg gaaggtctac ggcaaaaagg atggactatc ctcaatctac 9120
tggttattca aagagaggct aaaatcagaa acactgctgt caaagtcttg gcacaaggtg 9180
ataatcaagt tatttgcaca cagtataaaa cgaagaaatc gagaaacgtt gtagaattac 9240
agggtgctct caatcaaatg gtttctaata atgagaaaat tatgactgca atcaaaatag 9300
ggacagggaa gttaggactt ttgataaatg acgatgagac tatgcaatct gcagattact 9360
tgaattatgg aaaaataccg attttccgtg gagtgattag agggttagag accaagagat 9420
ggtcacgagt gacttgtgtc accaatgacc aaatacccac ttgtgctaat ataatgagct 9480
cagtttccac aaatgctctc accgtagctc attttgctga gaacccaatc aatgccatga 9540
tacagtacaa ttattttggg acatttgcta gactcttgtt gatgatgcat gatcctgctc 9600
ttcgtcaatc attgtatgaa gttcaagata agataccggg cttgcacagt tctactttca 9660
aatacgccat gttgtatttg gacccttcca ttggaggagt gtcgggcatg tctttgtcca 9720
ggtttttgat tagagccttc ccagatcccg taacagaaag tctctcattc tggagattca 9780
tccatgtaca tgctcgaagt gagcatctga aggagatgag tgcagtattt ggaaaccccg 9840
agatagccaa gtttcgaata actcacatag acaagctagt agaagatcca acctctctga 9900
acatcgctat gggaatgagt ccagcgaact tgttaaagac tgaggttaaa aaatgcttaa 9960
tcgaatcaag acaaaccatc aggaaccagg tgattaagga tgcaaccata tatttgtatc 10020
atgaagagga tcggctcaga agtttcttat ggtcaataaa tcctctgttc cctagatttt 10080
taagtgaatt caaatcaggc acttttttgg gagtcgcaga cgggctcatc agtctatttc 10140
aaaattctcg tactattcgg aactccttta agaaaaagta tcatagggaa ttggatgatt 10200
tgattgtgag gagtgaggta tcctctttga cacatttagg gaaacttcat ttgagaaggg 10260
gatcatgtaa aatgtggaca tgttcagcta ctcatgctga cacattaaga tacaaatcct 10320
ggggccgtac agttattggg acaactgtac cccatccatt agaaatgttg ggtccacaac 10380
atcgaaaaga gactccttgt gcaccatgta acacatcagg gttcaattat gtttctgtgc 10440
attgtccaga cgggatccat gacgtcttta gttcacgggg accattgcct gcttatctag 10500
ggtctaaaac atctgaatct acatctattt tgcagccttg ggaaagggaa agcaaagtcc 10560
cactgattaa aagagctaca cgtcttagag atgctatctc ttggtttgtt gaacccgact 10620
ctaaactagc aatgactata ctttctaaca tccactcttt aacaggcgaa gaatggacca 10680
aaaggcagca tgggttcaaa agaacagggt ctgcccttca taggttttcg acatctcgga 10740
tgagccatgg tgggttcgca tctcagagca ctgcagcatt gaccaggttg atggcaacta 10800
cagacaccat gagggatctg ggagatcaga atttcgactt tttattccaa gcaacgttgc 10860
tctatgctca aattaccacc actgttgcaa gagacggatg gatcaccagt tgtacagatc 10920
attatcatat tgcctgtaag tcctgtttga gacccataga agagatcacc ctggactcaa 10980
gtatggacta cacgccccca gatgtatccc atgtgctgaa gacatggagg aatggggaag 11040
gttcgtgggg acaagagata aaacagatct atcctttaga agggaattgg aagaatttag 11100
cacctgctga gcaatcctat caagtcggca gatgtatagg ttttctatat ggagacttgg 11160
cgtatagaaa atctactcat gccgaggaca gttctctatt tcctctatct atacaaggtc 11220
gtattagagg tcgaggtttc ttaaaagggt tgctagacgg attaatgaga gcaagttgct 11280
gccaagtaat acaccggaga agtctggctc atttgaagag gccggccaac gcagtgtacg 11340
gaggtttgat ttacttgatt gataaattga gtgtatcacc tccattcctt tctcttacta 11400
gatcaggacc tattagagac gaattagaaa cgattcccca caagatccca acctcctatc 11460
cgacaagcaa ccgtgatatg ggggtgattg tcagaaatta cttcaaatac caatgccgtc 11520
taattgaaaa gggaaaatac agatcacatt attcacaatt atggttattc tcagatgtct 11580
tatccataga cttcattgga ccattctcta tttccaccac cctcttgcaa atcctataca 11640
agccattttt atctgggaaa gataagaatg agttgagaga gctggcaaat ctttcttcat 11700
tgctaagatc aggagagggg tgggaagaca tacatgtgaa attcttcacc aaggacatat 11760
tattgtgtcc agaggaaatc agacatgctt gcaagttcgg gattgctaag gataataata 11820
aagacatgag ctatccccct tggggaaggg aatccagagg gacaattaca acaatccctg 11880
tttattatac gaccacccct tacccaaaga tgctagagat gcctccaaga atccaaaatc 11940
ccctgctgtc cggaatcagg ttgggccaat taccaactgg cgctcattat aaaattcgga 12000
gtatattaca tggaatggga atccattaca gggacttctt gagttgtgga gacggctccg 12060
gagggatgac tgctgcatta ctacgagaaa atgtgcatag cagaggaata ttcaatagtc 12120
tgttagaatt atcagggtca gtcatgcgag gcgcctctcc tgagcccccc agtgccctag 12180
aaactttagg aggagataaa tcgagatgtg taaatggtga aacatgttgg gaatatccat 12240
ctgacttatg tgacccaagg acttgggact atttcctccg actcaaagca ggcttggggc 12300
ttcaaattga tttaattgta atggatatgg aagttcggga ttcttctact agcctgaaaa 12360
ttgagacgaa tgttagaaat tatgtgcacc ggattttgga tgagcaagga gttttaatct 12420
acaagactta tggaacatat atttgtgaga gcgaaaagaa tgcagtaaca atccttggtc 12480
ccatgttcaa gacggtcgac ttagttcaaa cagaatttag tagttctcaa acgtctgaag 12540
tatatatggt atgtaaaggt ttgaagaaat taatcgatga acccaatccc gattggtctt 12600
ccatcaatga atcctggaaa aacctgtacg cattccagtc atcagaacag gaatttgcca 12660
gagcaaagaa ggttagtaca tactttacct tgacaggtat tccctcccaa ttcattcctg 12720
atccttttgt aaacattgag actatgctac aaatattcgg agtacccacg ggtgtgtctc 12780
atgcggctgc cttaaaatca tctgatagac ctgcagattt attgaccatt agcctttttt 12840
atatggcgat tatatcgtat tataacatca atcatatcag agtaggaccg atacctccga 12900
accccccatc agatggaatt gcacaaaatg tggggatcgc tataactggt ataagctttt 12960
ggctgagttt gatggagaaa gacattccac tatatcaaca gtgtttagca gttatccagc 13020
aatcattccc gattaggtgg gaggctgttt cagtaaaagg aggatacaag cagaagtgga 13080
gtactagagg tgatgggctc ccaaaagata cccgaacttc agactccttg gccccaatcg 13140
ggaactggat cagatctctg gaattggtcc gaaaccaagt tcgtctaaat ccattcaatg 13200
agatcttgtt caatcagcta tgtcgtacag tggataatca tttgaaatgg tcaaatttgc 13260
gaagaaacac aggaatgatt gaatggatca atagacgaat ttcaaaagaa gaccggtcta 13320
tactgatgtt gaagagtgac ctacacgagg aaaactcttg gagagattaa aaaatcatga 13380
ggagactcca aactttaagt atgaaaaaaa ctttgatcct taagaccctc ttgtggtttt 13440
tattttttat ctggttttgt ggtcttcgt 13469
<210> 20
<211> 13556
<212> DNA
<213> 人工序列
<220>
<223> VSV载体: Convac V2 China
<400> 20
acgaagacaa acaaaccatt attatcatta aaaggctcag gagaaacttt aacagtaatc 60
aaaatgtctg ttacagtcaa gagaatcatt gacaacacag tcatagttcc aaaacttcct 120
gcaaatgagg atccagtgga atacccggca gattacttca gaaaatcaaa ggagattcct 180
ctttacatca atactacaaa aagtttgtca gatctaagag gatatgtcta ccaaggcctc 240
aaatccggaa atgtatcaat catacatgtc aacagctact tgtatggagc attaaaggac 300
atccggggta agttggataa agattggtca agtttcggaa taaacatcgg gaaagcaggg 360
gatacaatcg gaatatttga ccttgtatcc ttgaaagccc tggacggcgt acttccagat 420
ggagtatcgg atgcttccag aaccagcgca gatgacaaat ggttgccttt gtatctactt 480
ggcttataca gagtgggcag aacacaaatg cctgaataca gaaaaaagct catggatggg 540
ctgacaaatc aatgcaaaat gatcaatgaa cagtttgaac ctcttgtgcc agaaggtcgt 600
gacatttttg atgtgtgggg aaatgacagt aattacacaa aaattgtcgc tgcagtggac 660
atgttcttcc acatgttcaa aaaacatgaa tgtgcctcgt tcagatacgg aactattgtt 720
tccagattca aagattgtgc tgcattggca acatttggac acctctgcaa aataaccgga 780
atgtctacag aagatgtaac gacctggatc ttgaaccgag aagttgcaga tgaaatggtc 840
caaatgatgc ttccaggcca agaaattgac aaggccgatt catacatgcc ttatttgatc 900
gactttggat tgtcttctaa gtctccatat tcttccgtca aaaaccctgc cttccacttc 960
tgggggcaat tgacagctct tctgctcaga tccaccagag caaggaatgc ccgacagcct 1020
gatgacattg agtatacatc tcttactaca gcaggtttgt tgtacgctta tgcagtagga 1080
tcctctgccg acttggcaca acagttttgt gttggagata acaaatacac tccagatgat 1140
agtaccggag gattgacgac taatgcaccg ccacaaggca gagatgtggt cgaatggctc 1200
ggatggtttg aagatcaaaa cagaaaaccg actcctgata tgatgcagta tgcgaaaaga 1260
gcagtcatgt cactgcaagg cctaagagag aagacaattg gcaagtatgc taagtcagaa 1320
tttgacaaat gaccctataa ttctcagatc acctattata tattatgcta catatgaaaa 1380
aaactaacag atatcatgga taatctcaca aaagttcgtg agtatctcaa gtcctattct 1440
cgtctggatc aggcggtagg agagatagat gagatcgaag cacaacgagc tgaaaagtcc 1500
aattatgagt tgttccaaga ggatggagtg gaagagcata ctaagccctc ttattttcag 1560
gcagcagatg attctgacac agaatctgaa ccagaaattg aagacaatca aggtttgtat 1620
gcaccagatc cagaagctga gcaagttgaa ggctttatac aggggccttt agatgactat 1680
gcagatgagg aagtggatgt tgtatttact tcggactgga aacagcctga gcttgaatct 1740
gacgagcatg gaaagacctt acggttgaca tcgccagagg gtttaagtgg agagcagaaa 1800
tcccagtggc tttcgacgat taaagcagtc gtgcaaagtg ccaaatactg gaatctggca 1860
gagtgcacat ttgaagcatc gggagaaggg gtcattatga aggagcgcca gataactccg 1920
gatgtatata aggtcactcc agtgatgaac acacatccgt cccaatcaga agcagtatca 1980
gatgtttggt ctctctcaaa gacatccatg actttccaac ccaagaaagc aagtcttcag 2040
cctctcacca tatccttgga tgaattgttc tcatctagag gagagttcat ctctgtcgga 2100
ggtgacggac gaatgtctca taaagaggcc atcctgctcg gcctgagata caaaaagttg 2160
tacaatcagg cgagagtcaa atattctctg tagactatga aaaaaagtaa cagatatcac 2220
gatctaagtg ttatcccaat ccattcatca tgagttcctt aaagaagatt ctcggtctga 2280
aggggaaagg taagaaatct aagaaattag ggatcgcacc acccccttat gaagaggaca 2340
ctagcatgga gtatgctccg agcgctccaa ttgacaaatc ctattttgga gttgacgaga 2400
tggacaccta tgatccgaat caattaagat atgagaaatt cttctttaca gtgaaaatga 2460
cggttagatc taatcgtccg ttcagaacat actcagatgt ggcagccgct gtatcccatt 2520
gggatcacat gtacatcgga atggcaggga aacgtccctt ctacaaaatc ttggcttttt 2580
tgggttcttc taatctaaag gccactccag cggtattggc agatcaaggt caaccagagt 2640
atcacactca ctgcgaaggc agggcttatt tgccacatag gatggggaag acccctccca 2700
tgctcaatgt accagagcac ttcagaagac cattcaatat aggtctttac aagggaacga 2760
ttgagctcac aatgaccatc tacgatgatg agtcactgga agcagctcct atgatctggg 2820
atcatttcaa ttcttccaaa ttttctgatt tcagagagaa ggccttaatg tttggcctga 2880
ttgtcgagaa aaaggcatct ggagcgtggg tcctggattc tatcagccac ttcaaatgag 2940
ctagtctaac ttctagcttc tgaacaatcc ccggtttact cagtctctcc taattccagc 3000
ctctcgaaca actaatatcc tgtcttttct atccctatga aaaaaactaa cagagatcga 3060
tctgtttacg cgtcactatg aagtgccttt tgtacttagc ctttttattc attggggtga 3120
attgcaagtt caccatagtt tttccacaca accaaaaagg aaactggaaa aatgttcctt 3180
ctaattacca ttattgcccg tcaagctcag atttaaattg gcataatgac ttaataggca 3240
cagccataca agtcaaaatg cccaagagtc acaaggctat tcaagcagac ggttggatgt 3300
gtcatgcttc caaatgggtc actacttgtg atttccgctg gtatggaccg aagtatataa 3360
cacagtccat ccgatccttc actccatctg tagaacaatg caaggaaagc attgaacaaa 3420
cgaaacaagg aacttggctg aatccaggct tccctcctca aagttgtgga tatgcaactg 3480
tgacggatgc cgaagcagtg attgtccagg tgactcctca ccatgtgctg gttgatgaat 3540
acacaggaga atgggttgat tcacagttca tcaacggaaa atgcagcaat tacatatgcc 3600
ccactgtcca taactctaca acctggcatt ctgactataa ggtcaaaggg ctatgtgatt 3660
ctaacctcat ttccatggac atcaccttct tctcagagga cggagagcta tcatccctgg 3720
gaaaggaggg cacagggttc agaagtaact actttgctta tgaaactgga ggcaaggcct 3780
gcaaaatgca atactgcaag cattggggag tcagactccc atcaggtgtc tggttcgaga 3840
tggctgataa ggatctcttt gctgcagcca gattccctga atgcccagaa gggtcaagta 3900
tctctgctcc atctcagacc tcagtggatg taagtctaat tcaggacgtt gagaggatct 3960
tggattattc cctctgccaa gaaacctgga gcaaaatcag agcgggtctt ccaatctctc 4020
cagtggatct cagctatctt gctcctaaaa acccaggaac cggtcctgct ttcaccataa 4080
tcaatggtac cctaaaatac tttgagacca gatacatcag agtcgatatt gctgctccaa 4140
tcctctcaag aatggtcgga atgatcagtg gaactaccac agaaagggaa ctgtgggatg 4200
actgggcacc atatgaagac gtggaaattg gacccaatgg agttctgagg accagttcag 4260
gatataagtt tcctttatac atgattggac atggtatgtt ggactccgat cttcatctta 4320
gctcaaaggc tcaggtgttc gaacatcctc acattcaaga cgctgcttcg caacttcctg 4380
atgatgagag tttatttttt ggtgatactg ggctatccaa aaatccaatc gagcttgtag 4440
aaggttggtt cagtagttgg aaaagctcta ttgcctcttt tttctttatc atagggttaa 4500
tcattggact attcttggtt ctccgagttg gtatccatct ttgcattaaa ttaaagcaca 4560
ccaagaaaag acagatttat acagacatag agatgaaccg acttggaaag taactcaaat 4620
cctgctaggt atgaaaaaaa ctaacagata tcacgctcga gcgtacgcca ccatgttcgt 4680
gtttctggtg ctgctgcctc tggtgagctc ccagtgcgtg aacctgacca caaggaccca 4740
gctgccccct gcctatacca attccttcac acggggcgtg tactatcccg acaaggtgtt 4800
ccggagcagc gtgctgcact ccacacagga tctgtttctg cctttctttt ctaacgtgac 4860
ctggttccac gccatccacg tgagcggcac caatggcaca aagcggttcg acaatccagt 4920
gctgcccttt aacgatggcg tgtacttcgc ctccaccgag aagtctaaca tcatcagagg 4980
ctggatcttt ggcaccacac tggacagcaa gacacagtcc ctgctgatcg tgaacaatgc 5040
caccaacgtg gtcatcaagg tgtgcgagtt ccagttttgt aatgatccat tcctgggcgt 5100
gtactatcac aagaacaata agtcttggat ggagagcgag tttcgcgtgt attcctctgc 5160
caacaattgc acatttgagt acgtgtccca gcccttcctg atggacctgg agggcaagca 5220
gggcaatttc aagaacctga gggagttcgt gtttaagaat atcgatggct acttcaaaat 5280
ctactccaag cacaccccaa tcaacctggt gcgcgacctg ccacagggct tctctgccct 5340
ggagccactg gtggatctgc ccatcggcat caacatcacc cggtttcaga cactgctggc 5400
cctgcacaga agctacctga caccaggcga cagctcctct ggatggaccg caggagcagc 5460
agcctactat gtgggctatc tgcagcccag gaccttcctg ctgaagtaca acgagaatgg 5520
caccatcaca gacgccgtgg attgcgccct ggatcccctg tctgagacca agtgtacact 5580
gaagagcttt accgtggaga agggcatcta tcagacaagc aatttcaggg tgcagcctac 5640
cgagtccatc gtgcgctttc ccaatatcac aaacctgtgc ccttttggcg aggtgttcaa 5700
cgcaacccgc ttcgccagcg tgtacgcctg gaataggaag cgcatctcca actgcgtggc 5760
cgactattct gtgctgtaca acagcgcctc cttctctacc tttaagtgct atggcgtgag 5820
ccccacaaag ctgaatgacc tgtgctttac caacgtgtac gccgattcct tcgtgatcag 5880
gggcgacgag gtgcgccaga tcgcaccagg acagacaggc aagatcgcag actacaatta 5940
taagctgcct gacgatttca ccggctgcgt gatcgcctgg aactctaaca atctggatag 6000
caaagtgggc ggcaactaca attatctgta ccggctgttt agaaagtcta atctgaagcc 6060
attcgagagg gacatctcca cagaaatcta ccaggccggc tctaccccct gcaatggcgt 6120
ggagggcttt aactgttatt tccctctgca gagctacggc ttccagccaa caaacggcgt 6180
gggctatcag ccctaccgcg tggtggtgct gtcttttgag ctgctgcacg cacctgcaac 6240
agtgtgcgga ccaaagaaga gcaccaatct ggtgaagaac aagtgcgtga acttcaactt 6300
caacggactg accggaacag gcgtgctgac cgagtccaac aagaagttcc tgccttttca 6360
gcagttcggc agggacatcg cagataccac agacgccgtg cgcgaccctc agaccctgga 6420
gatcctggac atcacaccat gctccttcgg cggcgtgtct gtgatcacac caggcaccaa 6480
tacaagcaac caggtggccg tgctgtatca ggacgtgaat tgtaccgagg tgccagtggc 6540
aatccacgca gatcagctga cccctacatg gcgggtgtac tctaccggca gcaacgtgtt 6600
ccagacaaga gccggatgcc tgatcggagc agagcacgtg aacaatagct atgagtgcga 6660
catccctatc ggcgccggca tctgtgcctc ctaccagacc cagacaaact ccccaaggtc 6720
tgtgggagat gaggccgaag actttgtgga agtccacctg cctgatgtgc ataaccaggt 6780
gtctggcgtc gacctgggac tgccaaattg gggcaagtac gtgctgctga gtgctggagc 6840
actgactgcc ctgatgctga tcattttcct gatgacctgc tgtcggcgcg tgaacagaag 6900
tgagcccact cagcacaatc tgcgaggaac cgggagagaa gtgtcagtca cacctcagag 6960
cgggaaaatc attagtagtt gggaatcaca taaaagcggg ggcgagacca ggctgtgagc 7020
tagccagatt cttcatgttt ggaccaaatc aacttgtgat accatgctca aagaggcctc 7080
aattatattt gagtttttaa tttttatgaa aaaaactaac agcaatcatg gaagtccacg 7140
attttgagac cgacgagttc aatgatttca atgaagatga ctatgccaca agagaattcc 7200
tgaatcccga tgagcgcatg acgtacttga atcatgctga ttacaatttg aattctcctc 7260
taattagtga tgatattgac aatttgatca ggaaattcaa ttctcttccg attccctcga 7320
tgtgggatag taagaactgg gatggagttc ttgagatgtt aacatcatgt caagccaatc 7380
ccatctcaac atctcagatg cataaatgga tgggaagttg gttaatgtct gataatcatg 7440
atgccagtca agggtatagt tttttacatg aagtggacaa agaggcagaa ataacatttg 7500
acgtggtgga gaccttcatc cgcggctggg gcaacaaacc aattgaatac atcaaaaagg 7560
aaagatggac tgactcattc aaaattctcg cttatttgtg tcaaaagttt ttggacttac 7620
acaagttgac attaatctta aatgctgtct ctgaggtgga attgctcaac ttggcgagga 7680
ctttcaaagg caaagtcaga agaagttctc atggaacgaa catatgcagg attagggttc 7740
ccagcttggg tcctactttt atttcagaag gatgggctta cttcaagaaa cttgatattc 7800
taatggaccg aaactttctg ttaatggtca aagatgtgat tatagggagg atgcaaacgg 7860
tgctatccat ggtatgtaga atagacaacc tgttctcaga gcaagacatc ttctcccttc 7920
taaatatcta cagaattgga gataaaattg tggagaggca gggaaatttt tcttatgact 7980
tgattaaaat ggtggaaccg atatgcaact tgaagctgat gaaattagca agagaatcaa 8040
ggcctttagt cccacaattc cctcattttg aaaatcatat caagacttct gttgatgaag 8100
gggcaaaaat tgaccgaggt ataagattcc tccatgatca gataatgagt gtgaaaacag 8160
tggatctcac actggtgatt tatggatcgt tcagacattg gggtcatcct tttatagatt 8220
attacactgg actagaaaaa ttacattccc aagtaaccat gaagaaagat attgatgtgt 8280
catatgcaaa agcacttgca agtgatttag ctcggattgt tctatttcaa cagttcaatg 8340
atcataaaaa gtggttcgtg aatggagact tgctccctca tgatcatccc tttaaaagtc 8400
atgttaaaga aaatacatgg cccacagctg ctcaagttca agattttgga gataaatggc 8460
atgaacttcc gctgattaaa tgttttgaaa tacccgactt actagaccca tcgataatat 8520
actctgacaa aagtcattca atgaataggt cagaggtgtt gaaacatgtc cgaatgaatc 8580
cgaacactcc tatccctagt aaaaaggtgt tgcagactat gttggacaca aaggctacca 8640
attggaaaga atttcttaaa gagattgatg agaagggctt agatgatgat gatctaatta 8700
ttggtcttaa aggaaaggag agggaactga agttggcagg tagatttttc tccctaatgt 8760
cttggaaatt gcgagaatac tttgtaatta ccgaatattt gataaagact catttcgtcc 8820
ctatgtttaa aggcctgaca atggcggacg atctaactgc agtcattaaa aagatgttag 8880
attcctcatc cggccaagga ttgaagtcat atgaggcaat ttgcatagcc aatcacattg 8940
attacgaaaa atggaataac caccaaagga agttatcaaa cggcccagtg ttccgagtta 9000
tgggccagtt cttaggttat ccatccttaa tcgagagaac tcatgaattt tttgagaaaa 9060
gtcttatata ctacaatgga agaccagact tgatgcgtgt tcacaacaac acactgatca 9120
attcaacctc ccaacgagtt tgttggcaag gacaagaggg tggactggaa ggtctacggc 9180
aaaaaggatg gactatcctc aatctactgg ttattcaaag agaggctaaa atcagaaaca 9240
ctgctgtcaa agtcttggca caaggtgata atcaagttat ttgcacacag tataaaacga 9300
agaaatcgag aaacgttgta gaattacagg gtgctctcaa tcaaatggtt tctaataatg 9360
agaaaattat gactgcaatc aaaataggga cagggaagtt aggacttttg ataaatgacg 9420
atgagactat gcaatctgca gattacttga attatggaaa aataccgatt ttccgtggag 9480
tgattagagg gttagagacc aagagatggt cacgagtgac ttgtgtcacc aatgaccaaa 9540
tacccacttg tgctaatata atgagctcag tttccacaaa tgctctcacc gtagctcatt 9600
ttgctgagaa cccaatcaat gccatgatac agtacaatta ttttgggaca tttgctagac 9660
tcttgttgat gatgcatgat cctgctcttc gtcaatcatt gtatgaagtt caagataaga 9720
taccgggctt gcacagttct actttcaaat acgccatgtt gtatttggac ccttccattg 9780
gaggagtgtc gggcatgtct ttgtccaggt ttttgattag agccttccca gatcccgtaa 9840
cagaaagtct ctcattctgg agattcatcc atgtacatgc tcgaagtgag catctgaagg 9900
agatgagtgc agtatttgga aaccccgaga tagccaagtt tcgaataact cacatagaca 9960
agctagtaga agatccaacc tctctgaaca tcgctatggg aatgagtcca gcgaacttgt 10020
taaagactga ggttaaaaaa tgcttaatcg aatcaagaca aaccatcagg aaccaggtga 10080
ttaaggatgc aaccatatat ttgtatcatg aagaggatcg gctcagaagt ttcttatggt 10140
caataaatcc tctgttccct agatttttaa gtgaattcaa atcaggcact tttttgggag 10200
tcgcagacgg gctcatcagt ctatttcaaa attctcgtac tattcggaac tcctttaaga 10260
aaaagtatca tagggaattg gatgatttga ttgtgaggag tgaggtatcc tctttgacac 10320
atttagggaa acttcatttg agaaggggat catgtaaaat gtggacatgt tcagctactc 10380
atgctgacac attaagatac aaatcctggg gccgtacagt tattgggaca actgtacccc 10440
atccattaga aatgttgggt ccacaacatc gaaaagagac tccttgtgca ccatgtaaca 10500
catcagggtt caattatgtt tctgtgcatt gtccagacgg gatccatgac gtctttagtt 10560
cacggggacc attgcctgct tatctagggt ctaaaacatc tgaatctaca tctattttgc 10620
agccttggga aagggaaagc aaagtcccac tgattaaaag agctacacgt cttagagatg 10680
ctatctcttg gtttgttgaa cccgactcta aactagcaat gactatactt tctaacatcc 10740
actctttaac aggcgaagaa tggaccaaaa ggcagcatgg gttcaaaaga acagggtctg 10800
cccttcatag gttttcgaca tctcggatga gccatggtgg gttcgcatct cagagcactg 10860
cagcattgac caggttgatg gcaactacag acaccatgag ggatctggga gatcagaatt 10920
tcgacttttt attccaagca acgttgctct atgctcaaat taccaccact gttgcaagag 10980
acggatggat caccagttgt acagatcatt atcatattgc ctgtaagtcc tgtttgagac 11040
ccatagaaga gatcaccctg gactcaagta tggactacac gcccccagat gtatcccatg 11100
tgctgaagac atggaggaat ggggaaggtt cgtggggaca agagataaaa cagatctatc 11160
ctttagaagg gaattggaag aatttagcac ctgctgagca atcctatcaa gtcggcagat 11220
gtataggttt tctatatgga gacttggcgt atagaaaatc tactcatgcc gaggacagtt 11280
ctctatttcc tctatctata caaggtcgta ttagaggtcg aggtttctta aaagggttgc 11340
tagacggatt aatgagagca agttgctgcc aagtaataca ccggagaagt ctggctcatt 11400
tgaagaggcc ggccaacgca gtgtacggag gtttgattta cttgattgat aaattgagtg 11460
tatcacctcc attcctttct cttactagat caggacctat tagagacgaa ttagaaacga 11520
ttccccacaa gatcccaacc tcctatccga caagcaaccg tgatatgggg gtgattgtca 11580
gaaattactt caaataccaa tgccgtctaa ttgaaaaggg aaaatacaga tcacattatt 11640
cacaattatg gttattctca gatgtcttat ccatagactt cattggacca ttctctattt 11700
ccaccaccct cttgcaaatc ctatacaagc catttttatc tgggaaagat aagaatgagt 11760
tgagagagct ggcaaatctt tcttcattgc taagatcagg agaggggtgg gaagacatac 11820
atgtgaaatt cttcaccaag gacatattat tgtgtccaga ggaaatcaga catgcttgca 11880
agttcgggat tgctaaggat aataataaag acatgagcta tcccccttgg ggaagggaat 11940
ccagagggac aattacaaca atccctgttt attatacgac caccccttac ccaaagatgc 12000
tagagatgcc tccaagaatc caaaatcccc tgctgtccgg aatcaggttg ggccaattac 12060
caactggcgc tcattataaa attcggagta tattacatgg aatgggaatc cattacaggg 12120
acttcttgag ttgtggagac ggctccggag ggatgactgc tgcattacta cgagaaaatg 12180
tgcatagcag aggaatattc aatagtctgt tagaattatc agggtcagtc atgcgaggcg 12240
cctctcctga gccccccagt gccctagaaa ctttaggagg agataaatcg agatgtgtaa 12300
atggtgaaac atgttgggaa tatccatctg acttatgtga cccaaggact tgggactatt 12360
tcctccgact caaagcaggc ttggggcttc aaattgattt aattgtaatg gatatggaag 12420
ttcgggattc ttctactagc ctgaaaattg agacgaatgt tagaaattat gtgcaccgga 12480
ttttggatga gcaaggagtt ttaatctaca agacttatgg aacatatatt tgtgagagcg 12540
aaaagaatgc agtaacaatc cttggtccca tgttcaagac ggtcgactta gttcaaacag 12600
aatttagtag ttctcaaacg tctgaagtat atatggtatg taaaggtttg aagaaattaa 12660
tcgatgaacc caatcccgat tggtcttcca tcaatgaatc ctggaaaaac ctgtacgcat 12720
tccagtcatc agaacaggaa tttgccagag caaagaaggt tagtacatac tttaccttga 12780
caggtattcc ctcccaattc attcctgatc cttttgtaaa cattgagact atgctacaaa 12840
tattcggagt acccacgggt gtgtctcatg cggctgcctt aaaatcatct gatagacctg 12900
cagatttatt gaccattagc cttttttata tggcgattat atcgtattat aacatcaatc 12960
atatcagagt aggaccgata cctccgaacc ccccatcaga tggaattgca caaaatgtgg 13020
ggatcgctat aactggtata agcttttggc tgagtttgat ggagaaagac attccactat 13080
atcaacagtg tttagcagtt atccagcaat cattcccgat taggtgggag gctgtttcag 13140
taaaaggagg atacaagcag aagtggagta ctagaggtga tgggctccca aaagataccc 13200
gaacttcaga ctccttggcc ccaatcggga actggatcag atctctggaa ttggtccgaa 13260
accaagttcg tctaaatcca ttcaatgaga tcttgttcaa tcagctatgt cgtacagtgg 13320
ataatcattt gaaatggtca aatttgcgaa gaaacacagg aatgattgaa tggatcaata 13380
gacgaatttc aaaagaagac cggtctatac tgatgttgaa gagtgaccta cacgaggaaa 13440
actcttggag agattaaaaa atcatgagga gactccaaac tttaagtatg aaaaaaactt 13500
tgatccttaa gaccctcttg tggtttttat tttttatctg gttttgtggt cttcgt 13556
<210> 21
<211> 13607
<212> DNA
<213> 人工序列
<220>
<223> VSV载体: Convac V2 South Africa
<400> 21
acgaagacaa acaaaccatt attatcatta aaaggctcag gagaaacttt aacagtaatc 60
aaaatgtctg ttacagtcaa gagaatcatt gacaacacag tcatagttcc aaaacttcct 120
gcaaatgagg atccagtgga atacccggca gattacttca gaaaatcaaa ggagattcct 180
ctttacatca atactacaaa aagtttgtca gatctaagag gatatgtcta ccaaggcctc 240
aaatccggaa atgtatcaat catacatgtc aacagctact tgtatggagc attaaaggac 300
atccggggta agttggataa agattggtca agtttcggaa taaacatcgg gaaagcaggg 360
gatacaatcg gaatatttga ccttgtatcc ttgaaagccc tggacggcgt acttccagat 420
ggagtatcgg atgcttccag aaccagcgca gatgacaaat ggttgccttt gtatctactt 480
ggcttataca gagtgggcag aacacaaatg cctgaataca gaaaaaagct catggatggg 540
ctgacaaatc aatgcaaaat gatcaatgaa cagtttgaac ctcttgtgcc agaaggtcgt 600
gacatttttg atgtgtgggg aaatgacagt aattacacaa aaattgtcgc tgcagtggac 660
atgttcttcc acatgttcaa aaaacatgaa tgtgcctcgt tcagatacgg aactattgtt 720
tccagattca aagattgtgc tgcattggca acatttggac acctctgcaa aataaccgga 780
atgtctacag aagatgtaac gacctggatc ttgaaccgag aagttgcaga tgaaatggtc 840
caaatgatgc ttccaggcca agaaattgac aaggccgatt catacatgcc ttatttgatc 900
gactttggat tgtcttctaa gtctccatat tcttccgtca aaaaccctgc cttccacttc 960
tgggggcaat tgacagctct tctgctcaga tccaccagag caaggaatgc ccgacagcct 1020
gatgacattg agtatacatc tcttactaca gcaggtttgt tgtacgctta tgcagtagga 1080
tcctctgccg acttggcaca acagttttgt gttggagata acaaatacac tccagatgat 1140
agtaccggag gattgacgac taatgcaccg ccacaaggca gagatgtggt cgaatggctc 1200
ggatggtttg aagatcaaaa cagaaaaccg actcctgata tgatgcagta tgcgaaaaga 1260
gcagtcatgt cactgcaagg cctaagagag aagacaattg gcaagtatgc taagtcagaa 1320
tttgacaaat gaccctataa ttctcagatc acctattata tattatgcta catatgaaaa 1380
aaactaacag atatcatgga taatctcaca aaagttcgtg agtatctcaa gtcctattct 1440
cgtctggatc aggcggtagg agagatagat gagatcgaag cacaacgagc tgaaaagtcc 1500
aattatgagt tgttccaaga ggatggagtg gaagagcata ctaagccctc ttattttcag 1560
gcagcagatg attctgacac agaatctgaa ccagaaattg aagacaatca aggtttgtat 1620
gcaccagatc cagaagctga gcaagttgaa ggctttatac aggggccttt agatgactat 1680
gcagatgagg aagtggatgt tgtatttact tcggactgga aacagcctga gcttgaatct 1740
gacgagcatg gaaagacctt acggttgaca tcgccagagg gtttaagtgg agagcagaaa 1800
tcccagtggc tttcgacgat taaagcagtc gtgcaaagtg ccaaatactg gaatctggca 1860
gagtgcacat ttgaagcatc gggagaaggg gtcattatga aggagcgcca gataactccg 1920
gatgtatata aggtcactcc agtgatgaac acacatccgt cccaatcaga agcagtatca 1980
gatgtttggt ctctctcaaa gacatccatg actttccaac ccaagaaagc aagtcttcag 2040
cctctcacca tatccttgga tgaattgttc tcatctagag gagagttcat ctctgtcgga 2100
ggtgacggac gaatgtctca taaagaggcc atcctgctcg gcctgagata caaaaagttg 2160
tacaatcagg cgagagtcaa atattctctg tagactatga aaaaaagtaa cagatatcac 2220
gatctaagtg ttatcccaat ccattcatca tgagttcctt aaagaagatt ctcggtctga 2280
aggggaaagg taagaaatct aagaaattag ggatcgcacc acccccttat gaagaggaca 2340
ctagcatgga gtatgctccg agcgctccaa ttgacaaatc ctattttgga gttgacgaga 2400
tggacaccta tgatccgaat caattaagat atgagaaatt cttctttaca gtgaaaatga 2460
cggttagatc taatcgtccg ttcagaacat actcagatgt ggcagccgct gtatcccatt 2520
gggatcacat gtacatcgga atggcaggga aacgtccctt ctacaaaatc ttggcttttt 2580
tgggttcttc taatctaaag gccactccag cggtattggc agatcaaggt caaccagagt 2640
atcacactca ctgcgaaggc agggcttatt tgccacatag gatggggaag acccctccca 2700
tgctcaatgt accagagcac ttcagaagac cattcaatat aggtctttac aagggaacga 2760
ttgagctcac aatgaccatc tacgatgatg agtcactgga agcagctcct atgatctggg 2820
atcatttcaa ttcttccaaa ttttctgatt tcagagagaa ggccttaatg tttggcctga 2880
ttgtcgagaa aaaggcatct ggagcgtggg tcctggattc tatcagccac ttcaaatgag 2940
ctagtctaac ttctagcttc tgaacaatcc ccggtttact cagtctctcc taattccagc 3000
ctctcgaaca actaatatcc tgtcttttct atccctatga aaaaaactaa cagagatcga 3060
tctgtttacg cgtcactatg aagtgccttt tgtacttagc ctttttattc attggggtga 3120
attgcaagtt caccatagtt tttccacaca accaaaaagg aaactggaaa aatgttcctt 3180
ctaattacca ttattgcccg tcaagctcag atttaaattg gcataatgac ttaataggca 3240
cagccataca agtcaaaatg cccaagagtc acaaggctat tcaagcagac ggttggatgt 3300
gtcatgcttc caaatgggtc actacttgtg atttccgctg gtatggaccg aagtatataa 3360
cacagtccat ccgatccttc actccatctg tagaacaatg caaggaaagc attgaacaaa 3420
cgaaacaagg aacttggctg aatccaggct tccctcctca aagttgtgga tatgcaactg 3480
tgacggatgc cgaagcagtg attgtccagg tgactcctca ccatgtgctg gttgatgaat 3540
acacaggaga atgggttgat tcacagttca tcaacggaaa atgcagcaat tacatatgcc 3600
ccactgtcca taactctaca acctggcatt ctgactataa ggtcaaaggg ctatgtgatt 3660
ctaacctcat ttccatggac atcaccttct tctcagagga cggagagcta tcatccctgg 3720
gaaaggaggg cacagggttc agaagtaact actttgctta tgaaactgga ggcaaggcct 3780
gcaaaatgca atactgcaag cattggggag tcagactccc atcaggtgtc tggttcgaga 3840
tggctgataa ggatctcttt gctgcagcca gattccctga atgcccagaa gggtcaagta 3900
tctctgctcc atctcagacc tcagtggatg taagtctaat tcaggacgtt gagaggatct 3960
tggattattc cctctgccaa gaaacctgga gcaaaatcag agcgggtctt ccaatctctc 4020
cagtggatct cagctatctt gctcctaaaa acccaggaac cggtcctgct ttcaccataa 4080
tcaatggtac cctaaaatac tttgagacca gatacatcag agtcgatatt gctgctccaa 4140
tcctctcaag aatggtcgga atgatcagtg gaactaccac agaaagggaa ctgtgggatg 4200
actgggcacc atatgaagac gtggaaattg gacccaatgg agttctgagg accagttcag 4260
gatataagtt tcctttatac atgattggac atggtatgtt ggactccgat cttcatctta 4320
gctcaaaggc tcaggtgttc gaacatcctc acattcaaga cgctgcttcg caacttcctg 4380
atgatgagag tttatttttt ggtgatactg ggctatccaa aaatccaatc gagcttgtag 4440
aaggttggtt cagtagttgg aaaagctcta ttgcctcttt tttctttatc atagggttaa 4500
tcattggact attcttggtt ctccgagttg gtatccatct ttgcattaaa ttaaagcaca 4560
ccaagaaaag acagatttat acagacatag agatgaaccg acttggaaag taactcaaat 4620
cctgctaggt atgaaaaaaa ctaacagata tcacgctcga gcgtacgcca ccatgttcgt 4680
gtttctggtg ctgctgcctc tggtgagctc ccagtgcgtg aacttcacca caaggaccca 4740
gctgccccct gcctatacca attccttcac acggggcgtg tactatcccg acaaggtgtt 4800
ccggagcagc gtgctgcact ccacacagga tctgtttctg cctttctttt ctaacgtgac 4860
ctggttccac gccatccacg tgagcggcac caatggcaca aagcggttcg ccaatccagt 4920
gctgcccttt aacgatggcg tgtacttcgc ctccaccgag aagtctaaca tcatcagagg 4980
ctggatcttt ggcaccacac tggacagcaa gacacagtcc ctgctgatcg tgaacaatgc 5040
caccaacgtg gtcatcaagg tgtgcgagtt ccagttttgt aatgatccat tcctgggcgt 5100
gtactatcac aagaacaata agtcttggat ggagagcgag tttcgcgtgt attcctctgc 5160
caacaattgc acatttgagt acgtgtccca gcccttcctg atggacctgg agggcaagca 5220
gggcaatttc aagaacctga gggagttcgt gtttaagaat atcgatggct acttcaaaat 5280
ctactccaag cacaccccaa tcaacctggt gcgcggcctg ccacagggct tctctgccct 5340
ggagccactg gtggatctgc ccatcggcat caacatcacc cggtttcaga cactgctggc 5400
cctgcacaga agctacctga caccaggcga cagctcctct ggatggaccg caggagcagc 5460
agcctactat gtgggctatc tgcagcccag gaccttcctg ctgaagtaca acgagaatgg 5520
caccatcaca gacgccgtgg attgcgccct ggatcccctg tctgagacca agtgtacact 5580
gaagagcttt accgtggaga agggcatcta tcagacaagc aatttcaggg tgcagcctac 5640
cgagtccatc gtgcgctttc ccaatatcac aaacctgtgc ccttttggcg aggtgttcaa 5700
cgcaacccgc ttcgccagcg tgtacgcctg gaataggaag cgcatctcca actgcgtggc 5760
cgactattct gtgctgtaca acagcgcctc cttctctacc tttaagtgct atggcgtgag 5820
ccccacaaag ctgaatgacc tgtgctttac caacgtgtac gccgattcct tcgtgatcag 5880
gggcgacgag gtgcgccaga tcgcaccagg acagacaggc aatatcgcag actacaatta 5940
taagctgcct gacgatttca ccggctgcgt gatcgcctgg aactctaaca atctggatag 6000
caaagtgggc ggcaactaca attatctgta ccggctgttt agaaagtcta atctgaagcc 6060
attcgagagg gacatctcca cagaaatcta ccaggccggc tctaccccct gcaatggcgt 6120
gaagggcttt aactgttatt tccctctgca gagctacggc ttccagccaa catatggcgt 6180
gggctatcag ccctaccgcg tggtggtgct gtcttttgag ctgctgcacg cacctgcaac 6240
agtgtgcgga ccaaagaaga gcaccaatct ggtgaagaac aagtgcgtga acttcaactt 6300
caacggactg accggaacag gcgtgctgac cgagtccaac aagaagttcc tgccttttca 6360
gcagttcggc agggacatcg cagataccac agacgccgtg cgcgaccctc agaccctgga 6420
gatcctggac atcacaccat gctccttcgg cggcgtgtct gtgatcacac caggcaccaa 6480
tacaagcaac caggtggccg tgctgtatca gggcgtgaat tgtaccgagg tgccagtggc 6540
aatccacgca gatcagctga cccctacatg gcgggtgtac tctaccggca gcaacgtgtt 6600
ccagacaaga gccggatgcc tgatcggagc agagcacgtg aacaatagct atgagtgcga 6660
catccctatc ggcgccggca tctgtgcctc ctaccagacc cagacaaact ccccagaatc 6720
aagcgtgatt cctctggtcc atccactggc agatccctcc acagtgttca aagacggaga 6780
tgaggccgaa gactttgtgg aagtccacct gcctgatgtg cataaccagg tgtctggcgt 6840
cgacctggga ctgccaaatt ggggcaagta cgtgctgctg agtgctggag cactgactgc 6900
cctgatgctg atcattttcc tgatgacctg ctgtcggcgc gtgaacagaa gtgagcccac 6960
tcagcacaat ctgcgaggaa ccgggagaga agtgtcagtc acacctcaga gcgggaaaat 7020
cattagtagt tgggaatcac ataaaagcgg gggcgagacc aggctgtgag ctagccagat 7080
tcttcatgtt tggaccaaat caacttgtga taccatgctc aaagaggcct caattatatt 7140
tgagttttta atttttatga aaaaaactaa cagcaatcat ggaagtccac gattttgaga 7200
ccgacgagtt caatgatttc aatgaagatg actatgccac aagagaattc ctgaatcccg 7260
atgagcgcat gacgtacttg aatcatgctg attacaattt gaattctcct ctaattagtg 7320
atgatattga caatttgatc aggaaattca attctcttcc gattccctcg atgtgggata 7380
gtaagaactg ggatggagtt cttgagatgt taacatcatg tcaagccaat cccatctcaa 7440
catctcagat gcataaatgg atgggaagtt ggttaatgtc tgataatcat gatgccagtc 7500
aagggtatag ttttttacat gaagtggaca aagaggcaga aataacattt gacgtggtgg 7560
agaccttcat ccgcggctgg ggcaacaaac caattgaata catcaaaaag gaaagatgga 7620
ctgactcatt caaaattctc gcttatttgt gtcaaaagtt tttggactta cacaagttga 7680
cattaatctt aaatgctgtc tctgaggtgg aattgctcaa cttggcgagg actttcaaag 7740
gcaaagtcag aagaagttct catggaacga acatatgcag gattagggtt cccagcttgg 7800
gtcctacttt tatttcagaa ggatgggctt acttcaagaa acttgatatt ctaatggacc 7860
gaaactttct gttaatggtc aaagatgtga ttatagggag gatgcaaacg gtgctatcca 7920
tggtatgtag aatagacaac ctgttctcag agcaagacat cttctccctt ctaaatatct 7980
acagaattgg agataaaatt gtggagaggc agggaaattt ttcttatgac ttgattaaaa 8040
tggtggaacc gatatgcaac ttgaagctga tgaaattagc aagagaatca aggcctttag 8100
tcccacaatt ccctcatttt gaaaatcata tcaagacttc tgttgatgaa ggggcaaaaa 8160
ttgaccgagg tataagattc ctccatgatc agataatgag tgtgaaaaca gtggatctca 8220
cactggtgat ttatggatcg ttcagacatt ggggtcatcc ttttatagat tattacactg 8280
gactagaaaa attacattcc caagtaacca tgaagaaaga tattgatgtg tcatatgcaa 8340
aagcacttgc aagtgattta gctcggattg ttctatttca acagttcaat gatcataaaa 8400
agtggttcgt gaatggagac ttgctccctc atgatcatcc ctttaaaagt catgttaaag 8460
aaaatacatg gcccacagct gctcaagttc aagattttgg agataaatgg catgaacttc 8520
cgctgattaa atgttttgaa atacccgact tactagaccc atcgataata tactctgaca 8580
aaagtcattc aatgaatagg tcagaggtgt tgaaacatgt ccgaatgaat ccgaacactc 8640
ctatccctag taaaaaggtg ttgcagacta tgttggacac aaaggctacc aattggaaag 8700
aatttcttaa agagattgat gagaagggct tagatgatga tgatctaatt attggtctta 8760
aaggaaagga gagggaactg aagttggcag gtagattttt ctccctaatg tcttggaaat 8820
tgcgagaata ctttgtaatt accgaatatt tgataaagac tcatttcgtc cctatgttta 8880
aaggcctgac aatggcggac gatctaactg cagtcattaa aaagatgtta gattcctcat 8940
ccggccaagg attgaagtca tatgaggcaa tttgcatagc caatcacatt gattacgaaa 9000
aatggaataa ccaccaaagg aagttatcaa acggcccagt gttccgagtt atgggccagt 9060
tcttaggtta tccatcctta atcgagagaa ctcatgaatt ttttgagaaa agtcttatat 9120
actacaatgg aagaccagac ttgatgcgtg ttcacaacaa cacactgatc aattcaacct 9180
cccaacgagt ttgttggcaa ggacaagagg gtggactgga aggtctacgg caaaaaggat 9240
ggactatcct caatctactg gttattcaaa gagaggctaa aatcagaaac actgctgtca 9300
aagtcttggc acaaggtgat aatcaagtta tttgcacaca gtataaaacg aagaaatcga 9360
gaaacgttgt agaattacag ggtgctctca atcaaatggt ttctaataat gagaaaatta 9420
tgactgcaat caaaataggg acagggaagt taggactttt gataaatgac gatgagacta 9480
tgcaatctgc agattacttg aattatggaa aaataccgat tttccgtgga gtgattagag 9540
ggttagagac caagagatgg tcacgagtga cttgtgtcac caatgaccaa atacccactt 9600
gtgctaatat aatgagctca gtttccacaa atgctctcac cgtagctcat tttgctgaga 9660
acccaatcaa tgccatgata cagtacaatt attttgggac atttgctaga ctcttgttga 9720
tgatgcatga tcctgctctt cgtcaatcat tgtatgaagt tcaagataag ataccgggct 9780
tgcacagttc tactttcaaa tacgccatgt tgtatttgga cccttccatt ggaggagtgt 9840
cgggcatgtc tttgtccagg tttttgatta gagccttccc agatcccgta acagaaagtc 9900
tctcattctg gagattcatc catgtacatg ctcgaagtga gcatctgaag gagatgagtg 9960
cagtatttgg aaaccccgag atagccaagt ttcgaataac tcacatagac aagctagtag 10020
aagatccaac ctctctgaac atcgctatgg gaatgagtcc agcgaacttg ttaaagactg 10080
aggttaaaaa atgcttaatc gaatcaagac aaaccatcag gaaccaggtg attaaggatg 10140
caaccatata tttgtatcat gaagaggatc ggctcagaag tttcttatgg tcaataaatc 10200
ctctgttccc tagattttta agtgaattca aatcaggcac ttttttggga gtcgcagacg 10260
ggctcatcag tctatttcaa aattctcgta ctattcggaa ctcctttaag aaaaagtatc 10320
atagggaatt ggatgatttg attgtgagga gtgaggtatc ctctttgaca catttaggga 10380
aacttcattt gagaagggga tcatgtaaaa tgtggacatg ttcagctact catgctgaca 10440
cattaagata caaatcctgg ggccgtacag ttattgggac aactgtaccc catccattag 10500
aaatgttggg tccacaacat cgaaaagaga ctccttgtgc accatgtaac acatcagggt 10560
tcaattatgt ttctgtgcat tgtccagacg ggatccatga cgtctttagt tcacggggac 10620
cattgcctgc ttatctaggg tctaaaacat ctgaatctac atctattttg cagccttggg 10680
aaagggaaag caaagtccca ctgattaaaa gagctacacg tcttagagat gctatctctt 10740
ggtttgttga acccgactct aaactagcaa tgactatact ttctaacatc cactctttaa 10800
caggcgaaga atggaccaaa aggcagcatg ggttcaaaag aacagggtct gcccttcata 10860
ggttttcgac atctcggatg agccatggtg ggttcgcatc tcagagcact gcagcattga 10920
ccaggttgat ggcaactaca gacaccatga gggatctggg agatcagaat ttcgactttt 10980
tattccaagc aacgttgctc tatgctcaaa ttaccaccac tgttgcaaga gacggatgga 11040
tcaccagttg tacagatcat tatcatattg cctgtaagtc ctgtttgaga cccatagaag 11100
agatcaccct ggactcaagt atggactaca cgcccccaga tgtatcccat gtgctgaaga 11160
catggaggaa tggggaaggt tcgtggggac aagagataaa acagatctat cctttagaag 11220
ggaattggaa gaatttagca cctgctgagc aatcctatca agtcggcaga tgtataggtt 11280
ttctatatgg agacttggcg tatagaaaat ctactcatgc cgaggacagt tctctatttc 11340
ctctatctat acaaggtcgt attagaggtc gaggtttctt aaaagggttg ctagacggat 11400
taatgagagc aagttgctgc caagtaatac accggagaag tctggctcat ttgaagaggc 11460
cggccaacgc agtgtacgga ggtttgattt acttgattga taaattgagt gtatcacctc 11520
cattcctttc tcttactaga tcaggaccta ttagagacga attagaaacg attccccaca 11580
agatcccaac ctcctatccg acaagcaacc gtgatatggg ggtgattgtc agaaattact 11640
tcaaatacca atgccgtcta attgaaaagg gaaaatacag atcacattat tcacaattat 11700
ggttattctc agatgtctta tccatagact tcattggacc attctctatt tccaccaccc 11760
tcttgcaaat cctatacaag ccatttttat ctgggaaaga taagaatgag ttgagagagc 11820
tggcaaatct ttcttcattg ctaagatcag gagaggggtg ggaagacata catgtgaaat 11880
tcttcaccaa ggacatatta ttgtgtccag aggaaatcag acatgcttgc aagttcggga 11940
ttgctaagga taataataaa gacatgagct atcccccttg gggaagggaa tccagaggga 12000
caattacaac aatccctgtt tattatacga ccacccctta cccaaagatg ctagagatgc 12060
ctccaagaat ccaaaatccc ctgctgtccg gaatcaggtt gggccaatta ccaactggcg 12120
ctcattataa aattcggagt atattacatg gaatgggaat ccattacagg gacttcttga 12180
gttgtggaga cggctccgga gggatgactg ctgcattact acgagaaaat gtgcatagca 12240
gaggaatatt caatagtctg ttagaattat cagggtcagt catgcgaggc gcctctcctg 12300
agccccccag tgccctagaa actttaggag gagataaatc gagatgtgta aatggtgaaa 12360
catgttggga atatccatct gacttatgtg acccaaggac ttgggactat ttcctccgac 12420
tcaaagcagg cttggggctt caaattgatt taattgtaat ggatatggaa gttcgggatt 12480
cttctactag cctgaaaatt gagacgaatg ttagaaatta tgtgcaccgg attttggatg 12540
agcaaggagt tttaatctac aagacttatg gaacatatat ttgtgagagc gaaaagaatg 12600
cagtaacaat ccttggtccc atgttcaaga cggtcgactt agttcaaaca gaatttagta 12660
gttctcaaac gtctgaagta tatatggtat gtaaaggttt gaagaaatta atcgatgaac 12720
ccaatcccga ttggtcttcc atcaatgaat cctggaaaaa cctgtacgca ttccagtcat 12780
cagaacagga atttgccaga gcaaagaagg ttagtacata ctttaccttg acaggtattc 12840
cctcccaatt cattcctgat ccttttgtaa acattgagac tatgctacaa atattcggag 12900
tacccacggg tgtgtctcat gcggctgcct taaaatcatc tgatagacct gcagatttat 12960
tgaccattag ccttttttat atggcgatta tatcgtatta taacatcaat catatcagag 13020
taggaccgat acctccgaac cccccatcag atggaattgc acaaaatgtg gggatcgcta 13080
taactggtat aagcttttgg ctgagtttga tggagaaaga cattccacta tatcaacagt 13140
gtttagcagt tatccagcaa tcattcccga ttaggtggga ggctgtttca gtaaaaggag 13200
gatacaagca gaagtggagt actagaggtg atgggctccc aaaagatacc cgaacttcag 13260
actccttggc cccaatcggg aactggatca gatctctgga attggtccga aaccaagttc 13320
gtctaaatcc attcaatgag atcttgttca atcagctatg tcgtacagtg gataatcatt 13380
tgaaatggtc aaatttgcga agaaacacag gaatgattga atggatcaat agacgaattt 13440
caaaagaaga ccggtctata ctgatgttga agagtgacct acacgaggaa aactcttgga 13500
gagattaaaa aatcatgagg agactccaaa ctttaagtat gaaaaaaact ttgatcctta 13560
agaccctctt gtggttttta ttttttatct ggttttgtgg tcttcgt 13607
<210> 22
<211> 13506
<212> DNA
<213> 人工序列
<220>
<223> VSV载体: Convac V3 China
<400> 22
acgaagacaa acaaaccatt attatcatta aaaggctcag gagaaacttt aacagtaatc 60
aaaatgtctg ttacagtcaa gagaatcatt gacaacacag tcatagttcc aaaacttcct 120
gcaaatgagg atccagtgga atacccggca gattacttca gaaaatcaaa ggagattcct 180
ctttacatca atactacaaa aagtttgtca gatctaagag gatatgtcta ccaaggcctc 240
aaatccggaa atgtatcaat catacatgtc aacagctact tgtatggagc attaaaggac 300
atccggggta agttggataa agattggtca agtttcggaa taaacatcgg gaaagcaggg 360
gatacaatcg gaatatttga ccttgtatcc ttgaaagccc tggacggcgt acttccagat 420
ggagtatcgg atgcttccag aaccagcgca gatgacaaat ggttgccttt gtatctactt 480
ggcttataca gagtgggcag aacacaaatg cctgaataca gaaaaaagct catggatggg 540
ctgacaaatc aatgcaaaat gatcaatgaa cagtttgaac ctcttgtgcc agaaggtcgt 600
gacatttttg atgtgtgggg aaatgacagt aattacacaa aaattgtcgc tgcagtggac 660
atgttcttcc acatgttcaa aaaacatgaa tgtgcctcgt tcagatacgg aactattgtt 720
tccagattca aagattgtgc tgcattggca acatttggac acctctgcaa aataaccgga 780
atgtctacag aagatgtaac gacctggatc ttgaaccgag aagttgcaga tgaaatggtc 840
caaatgatgc ttccaggcca agaaattgac aaggccgatt catacatgcc ttatttgatc 900
gactttggat tgtcttctaa gtctccatat tcttccgtca aaaaccctgc cttccacttc 960
tgggggcaat tgacagctct tctgctcaga tccaccagag caaggaatgc ccgacagcct 1020
gatgacattg agtatacatc tcttactaca gcaggtttgt tgtacgctta tgcagtagga 1080
tcctctgccg acttggcaca acagttttgt gttggagata acaaatacac tccagatgat 1140
agtaccggag gattgacgac taatgcaccg ccacaaggca gagatgtggt cgaatggctc 1200
ggatggtttg aagatcaaaa cagaaaaccg actcctgata tgatgcagta tgcgaaaaga 1260
gcagtcatgt cactgcaagg cctaagagag aagacaattg gcaagtatgc taagtcagaa 1320
tttgacaaat gaccctataa ttctcagatc acctattata tattatgcta catatgaaaa 1380
aaactaacag atatcatgga taatctcaca aaagttcgtg agtatctcaa gtcctattct 1440
cgtctggatc aggcggtagg agagatagat gagatcgaag cacaacgagc tgaaaagtcc 1500
aattatgagt tgttccaaga ggatggagtg gaagagcata ctaagccctc ttattttcag 1560
gcagcagatg attctgacac agaatctgaa ccagaaattg aagacaatca aggtttgtat 1620
gcaccagatc cagaagctga gcaagttgaa ggctttatac aggggccttt agatgactat 1680
gcagatgagg aagtggatgt tgtatttact tcggactgga aacagcctga gcttgaatct 1740
gacgagcatg gaaagacctt acggttgaca tcgccagagg gtttaagtgg agagcagaaa 1800
tcccagtggc tttcgacgat taaagcagtc gtgcaaagtg ccaaatactg gaatctggca 1860
gagtgcacat ttgaagcatc gggagaaggg gtcattatga aggagcgcca gataactccg 1920
gatgtatata aggtcactcc agtgatgaac acacatccgt cccaatcaga agcagtatca 1980
gatgtttggt ctctctcaaa gacatccatg actttccaac ccaagaaagc aagtcttcag 2040
cctctcacca tatccttgga tgaattgttc tcatctagag gagagttcat ctctgtcgga 2100
ggtgacggac gaatgtctca taaagaggcc atcctgctcg gcctgagata caaaaagttg 2160
tacaatcagg cgagagtcaa atattctctg tagactatga aaaaaagtaa cagatatcac 2220
gatctaagtg ttatcccaat ccattcatca tgagttcctt aaagaagatt ctcggtctga 2280
aggggaaagg taagaaatct aagaaattag ggatcgcacc acccccttat gaagaggaca 2340
ctagcatgga gtatgctccg agcgctccaa ttgacaaatc ctattttgga gttgacgaga 2400
tggacaccta tgatccgaat caattaagat atgagaaatt cttctttaca gtgaaaatga 2460
cggttagatc taatcgtccg ttcagaacat actcagatgt ggcagccgct gtatcccatt 2520
gggatcacat gtacatcgga atggcaggga aacgtccctt ctacaaaatc ttggcttttt 2580
tgggttcttc taatctaaag gccactccag cggtattggc agatcaaggt caaccagagt 2640
atcacactca ctgcgaaggc agggcttatt tgccacatag gatggggaag acccctccca 2700
tgctcaatgt accagagcac ttcagaagac cattcaatat aggtctttac aagggaacga 2760
ttgagctcac aatgaccatc tacgatgatg agtcactgga agcagctcct atgatctggg 2820
atcatttcaa ttcttccaaa ttttctgatt tcagagagaa ggccttaatg tttggcctga 2880
ttgtcgagaa aaaggcatct ggagcgtggg tcctggattc tatcagccac ttcaaatgag 2940
ctagtctaac ttctagcttc tgaacaatcc ccggtttact cagtctctcc taattccagc 3000
ctctcgaaca actaatatcc tgtcttttct atccctatga aaaaaactaa cagagatcga 3060
tctgtttacg cgtcactatg aagtgccttt tgtacttagc ctttttattc attggggtga 3120
attgcaagtt caccatagtt tttccacaca accaaaaagg aaactggaaa aatgttcctt 3180
ctaattacca ttattgcccg tcaagctcag atttaaattg gcataatgac ttaataggca 3240
cagccataca agtcaaaatg cccaagagtc acaaggctat tcaagcagac ggttggatgt 3300
gtcatgcttc caaatgggtc actacttgtg atttccgctg gtatggaccg aagtatataa 3360
cacagtccat ccgatccttc actccatctg tagaacaatg caaggaaagc attgaacaaa 3420
cgaaacaagg aacttggctg aatccaggct tccctcctca aagttgtgga tatgcaactg 3480
tgacggatgc cgaagcagtg attgtccagg tgactcctca ccatgtgctg gttgatgaat 3540
acacaggaga atgggttgat tcacagttca tcaacggaaa atgcagcaat tacatatgcc 3600
ccactgtcca taactctaca acctggcatt ctgactataa ggtcaaaggg ctatgtgatt 3660
ctaacctcat ttccatggac atcaccttct tctcagagga cggagagcta tcatccctgg 3720
gaaaggaggg cacagggttc agaagtaact actttgctta tgaaactgga ggcaaggcct 3780
gcaaaatgca atactgcaag cattggggag tcagactccc atcaggtgtc tggttcgaga 3840
tggctgataa ggatctcttt gctgcagcca gattccctga atgcccagaa gggtcaagta 3900
tctctgctcc atctcagacc tcagtggatg taagtctaat tcaggacgtt gagaggatct 3960
tggattattc cctctgccaa gaaacctgga gcaaaatcag agcgggtctt ccaatctctc 4020
cagtggatct cagctatctt gctcctaaaa acccaggaac cggtcctgct ttcaccataa 4080
tcaatggtac cctaaaatac tttgagacca gatacatcag agtcgatatt gctgctccaa 4140
tcctctcaag aatggtcgga atgatcagtg gaactaccac agaaagggaa ctgtgggatg 4200
actgggcacc atatgaagac gtggaaattg gacccaatgg agttctgagg accagttcag 4260
gatataagtt tcctttatac atgattggac atggtatgtt ggactccgat cttcatctta 4320
gctcaaaggc tcaggtgttc gaacatcctc acattcaaga cgctgcttcg caacttcctg 4380
atgatgagag tttatttttt ggtgatactg ggctatccaa aaatccaatc gagcttgtag 4440
aaggttggtt cagtagttgg aaaagctcta ttgcctcttt tttctttatc atagggttaa 4500
tcattggact attcttggtt ctccgagttg gtatccatct ttgcattaaa ttaaagcaca 4560
ccaagaaaag acagatttat acagacatag agatgaaccg acttggaaag taactcaaat 4620
cctgctaggt atgaaaaaaa ctaacagata tcacgctcga ggccaccatg ttcgtgtttc 4680
tggtgctgct gcctctggtg agctcccagt gcgtgaacct gaccacaagg acccagctgc 4740
cccctgccta taccaattcc ttcacacggg gcgtgtacta tcccgacaag gtgttccgga 4800
gcagcgtgct gcactccaca caggatctgt ttctgccttt cttttctaac gtgacctggt 4860
tccacgccat ccacgtgagc ggcaccaatg gcacaaagcg gttcgacaat ccagtgctgc 4920
cctttaacga tggcgtgtac ttcgcctcca ccgagaagtc taacatcatc agaggctgga 4980
tctttggcac cacactggac agcaagacac agtccctgct gatcgtgaac aatgccacca 5040
acgtggtcat caaggtgtgc gagttccagt tttgtaatga tccattcctg ggcgtgtact 5100
atcacaagaa caataagtct tggatggaga gcgagtttcg cgtgtattcc tctgccaaca 5160
attgcacatt tgagtacgtg tcccagccct tcctgatgga cctggagggc aagcagggca 5220
atttcaagaa cctgagggag ttcgtgttta agaatatcga tggctacttc aaaatctact 5280
ccaagcacac cccaatcaac ctggtgcgcg acctgccaca gggcttctct gccctggagc 5340
cactggtgga tctgcccatc ggcatcaaca tcacccggtt tcagacactg ctggccctgc 5400
acagaagcta cctgacacca ggcgacagct cctctggatg gaccgcagga gcagcagcct 5460
actatgtggg ctatctgcag cccaggacct tcctgctgaa gtacaacgag aatggcacca 5520
tcacagacgc cgtggattgc gccctggatc ccctgtctga gaccaagtgt acactgaaga 5580
gctttaccgt ggagaagggc atctatcaga caagcaattt cagggtgcag cctaccgagt 5640
ccatcgtgcg ctttcccaat atcacaaacc tgtgcccttt tggcgaggtg ttcaacgcaa 5700
cccgcttcgc cagcgtgtac gcctggaata ggaagcgcat ctccaactgc gtggccgact 5760
attctgtgct gtacaacagc gcctccttct ctacctttaa gtgctatggc gtgagcccca 5820
caaagctgaa tgacctgtgc tttaccaacg tgtacgccga ttccttcgtg atcaggggcg 5880
acgaggtgcg ccagatcgca ccaggacaga caggcaagat cgcagactac aattataagc 5940
tgcctgacga tttcaccggc tgcgtgatcg cctggaactc taacaatctg gatagcaaag 6000
tgggcggcaa ctacaattat ctgtaccggc tgtttagaaa gtctaatctg aagccattcg 6060
agagggacat ctccacagaa atctaccagg ccggctctac cccctgcaat ggcgtggagg 6120
gctttaactg ttatttccct ctgcagagct acggcttcca gccaacaaac ggcgtgggct 6180
atcagcccta ccgcgtggtg gtgctgtctt ttgagctgct gcacgcacct gcaacagtgt 6240
gcggaccaaa gaagagcacc aatctggtga agaacaagtg cgtgaacttc aacttcaacg 6300
gactgaccgg aacaggcgtg ctgaccgagt ccaacaagaa gttcctgcct tttcagcagt 6360
tcggcaggga catcgcagat accacagacg ccgtgcgcga ccctcagacc ctggagatcc 6420
tggacatcac accatgctcc ttcggcggcg tgtctgtgat cacaccaggc accaatacaa 6480
gcaaccaggt ggccgtgctg tatcaggacg tgaattgtac cgaggtgcca gtggcaatcc 6540
acgcagatca gctgacccct acatggcggg tgtactctac cggcagcaac gtgttccaga 6600
caagagccgg atgcctgatc ggagcagagc acgtgaacaa tagctatgag tgcgacatcc 6660
ctatcggcgc cggcatctgt gcctcctacc agacccagac aaactcccca aggtctggat 6720
ccggctacat ccccgaggcc cccagagacg gccaggccta cgtgcggaag gacggcgagt 6780
gggtactgct cagcaccttc ctgggcagca gttggaaaag ctccatcgcc tcctttttct 6840
ttatcatcgg cctgatcatc ggactgttcc tggtgctccg cgtgggtatc cacctgtgca 6900
tcaagctgaa gcacaccaag aaaagacaga tttatacaga catcgagatg aaccgacttg 6960
gaaagtaagc tagccagatt cttcatgttt ggaccaaatc aacttgtgat accatgctca 7020
aagaggcctc aattatattt gagtttttaa tttttatgaa aaaaactaac agcaatcatg 7080
gaagtccacg attttgagac cgacgagttc aatgatttca atgaagatga ctatgccaca 7140
agagaattcc tgaatcccga tgagcgcatg acgtacttga atcatgctga ttacaatttg 7200
aattctcctc taattagtga tgatattgac aatttgatca ggaaattcaa ttctcttccg 7260
attccctcga tgtgggatag taagaactgg gatggagttc ttgagatgtt aacatcatgt 7320
caagccaatc ccatctcaac atctcagatg cataaatgga tgggaagttg gttaatgtct 7380
gataatcatg atgccagtca agggtatagt tttttacatg aagtggacaa agaggcagaa 7440
ataacatttg acgtggtgga gaccttcatc cgcggctggg gcaacaaacc aattgaatac 7500
atcaaaaagg aaagatggac tgactcattc aaaattctcg cttatttgtg tcaaaagttt 7560
ttggacttac acaagttgac attaatctta aatgctgtct ctgaggtgga attgctcaac 7620
ttggcgagga ctttcaaagg caaagtcaga agaagttctc atggaacgaa catatgcagg 7680
attagggttc ccagcttggg tcctactttt atttcagaag gatgggctta cttcaagaaa 7740
cttgatattc taatggaccg aaactttctg ttaatggtca aagatgtgat tatagggagg 7800
atgcaaacgg tgctatccat ggtatgtaga atagacaacc tgttctcaga gcaagacatc 7860
ttctcccttc taaatatcta cagaattgga gataaaattg tggagaggca gggaaatttt 7920
tcttatgact tgattaaaat ggtggaaccg atatgcaact tgaagctgat gaaattagca 7980
agagaatcaa ggcctttagt cccacaattc cctcattttg aaaatcatat caagacttct 8040
gttgatgaag gggcaaaaat tgaccgaggt ataagattcc tccatgatca gataatgagt 8100
gtgaaaacag tggatctcac actggtgatt tatggatcgt tcagacattg gggtcatcct 8160
tttatagatt attacactgg actagaaaaa ttacattccc aagtaaccat gaagaaagat 8220
attgatgtgt catatgcaaa agcacttgca agtgatttag ctcggattgt tctatttcaa 8280
cagttcaatg atcataaaaa gtggttcgtg aatggagact tgctccctca tgatcatccc 8340
tttaaaagtc atgttaaaga aaatacatgg cccacagctg ctcaagttca agattttgga 8400
gataaatggc atgaacttcc gctgattaaa tgttttgaaa tacccgactt actagaccca 8460
tcgataatat actctgacaa aagtcattca atgaataggt cagaggtgtt gaaacatgtc 8520
cgaatgaatc cgaacactcc tatccctagt aaaaaggtgt tgcagactat gttggacaca 8580
aaggctacca attggaaaga atttcttaaa gagattgatg agaagggctt agatgatgat 8640
gatctaatta ttggtcttaa aggaaaggag agggaactga agttggcagg tagatttttc 8700
tccctaatgt cttggaaatt gcgagaatac tttgtaatta ccgaatattt gataaagact 8760
catttcgtcc ctatgtttaa aggcctgaca atggcggacg atctaactgc agtcattaaa 8820
aagatgttag attcctcatc cggccaagga ttgaagtcat atgaggcaat ttgcatagcc 8880
aatcacattg attacgaaaa atggaataac caccaaagga agttatcaaa cggcccagtg 8940
ttccgagtta tgggccagtt cttaggttat ccatccttaa tcgagagaac tcatgaattt 9000
tttgagaaaa gtcttatata ctacaatgga agaccagact tgatgcgtgt tcacaacaac 9060
acactgatca attcaacctc ccaacgagtt tgttggcaag gacaagaggg tggactggaa 9120
ggtctacggc aaaaaggatg gactatcctc aatctactgg ttattcaaag agaggctaaa 9180
atcagaaaca ctgctgtcaa agtcttggca caaggtgata atcaagttat ttgcacacag 9240
tataaaacga agaaatcgag aaacgttgta gaattacagg gtgctctcaa tcaaatggtt 9300
tctaataatg agaaaattat gactgcaatc aaaataggga cagggaagtt aggacttttg 9360
ataaatgacg atgagactat gcaatctgca gattacttga attatggaaa aataccgatt 9420
ttccgtggag tgattagagg gttagagacc aagagatggt cacgagtgac ttgtgtcacc 9480
aatgaccaaa tacccacttg tgctaatata atgagctcag tttccacaaa tgctctcacc 9540
gtagctcatt ttgctgagaa cccaatcaat gccatgatac agtacaatta ttttgggaca 9600
tttgctagac tcttgttgat gatgcatgat cctgctcttc gtcaatcatt gtatgaagtt 9660
caagataaga taccgggctt gcacagttct actttcaaat acgccatgtt gtatttggac 9720
ccttccattg gaggagtgtc gggcatgtct ttgtccaggt ttttgattag agccttccca 9780
gatcccgtaa cagaaagtct ctcattctgg agattcatcc atgtacatgc tcgaagtgag 9840
catctgaagg agatgagtgc agtatttgga aaccccgaga tagccaagtt tcgaataact 9900
cacatagaca agctagtaga agatccaacc tctctgaaca tcgctatggg aatgagtcca 9960
gcgaacttgt taaagactga ggttaaaaaa tgcttaatcg aatcaagaca aaccatcagg 10020
aaccaggtga ttaaggatgc aaccatatat ttgtatcatg aagaggatcg gctcagaagt 10080
ttcttatggt caataaatcc tctgttccct agatttttaa gtgaattcaa atcaggcact 10140
tttttgggag tcgcagacgg gctcatcagt ctatttcaaa attctcgtac tattcggaac 10200
tcctttaaga aaaagtatca tagggaattg gatgatttga ttgtgaggag tgaggtatcc 10260
tctttgacac atttagggaa acttcatttg agaaggggat catgtaaaat gtggacatgt 10320
tcagctactc atgctgacac attaagatac aaatcctggg gccgtacagt tattgggaca 10380
actgtacccc atccattaga aatgttgggt ccacaacatc gaaaagagac tccttgtgca 10440
ccatgtaaca catcagggtt caattatgtt tctgtgcatt gtccagacgg gatccatgac 10500
gtctttagtt cacggggacc attgcctgct tatctagggt ctaaaacatc tgaatctaca 10560
tctattttgc agccttggga aagggaaagc aaagtcccac tgattaaaag agctacacgt 10620
cttagagatg ctatctcttg gtttgttgaa cccgactcta aactagcaat gactatactt 10680
tctaacatcc actctttaac aggcgaagaa tggaccaaaa ggcagcatgg gttcaaaaga 10740
acagggtctg cccttcatag gttttcgaca tctcggatga gccatggtgg gttcgcatct 10800
cagagcactg cagcattgac caggttgatg gcaactacag acaccatgag ggatctggga 10860
gatcagaatt tcgacttttt attccaagca acgttgctct atgctcaaat taccaccact 10920
gttgcaagag acggatggat caccagttgt acagatcatt atcatattgc ctgtaagtcc 10980
tgtttgagac ccatagaaga gatcaccctg gactcaagta tggactacac gcccccagat 11040
gtatcccatg tgctgaagac atggaggaat ggggaaggtt cgtggggaca agagataaaa 11100
cagatctatc ctttagaagg gaattggaag aatttagcac ctgctgagca atcctatcaa 11160
gtcggcagat gtataggttt tctatatgga gacttggcgt atagaaaatc tactcatgcc 11220
gaggacagtt ctctatttcc tctatctata caaggtcgta ttagaggtcg aggtttctta 11280
aaagggttgc tagacggatt aatgagagca agttgctgcc aagtaataca ccggagaagt 11340
ctggctcatt tgaagaggcc ggccaacgca gtgtacggag gtttgattta cttgattgat 11400
aaattgagtg tatcacctcc attcctttct cttactagat caggacctat tagagacgaa 11460
ttagaaacga ttccccacaa gatcccaacc tcctatccga caagcaaccg tgatatgggg 11520
gtgattgtca gaaattactt caaataccaa tgccgtctaa ttgaaaaggg aaaatacaga 11580
tcacattatt cacaattatg gttattctca gatgtcttat ccatagactt cattggacca 11640
ttctctattt ccaccaccct cttgcaaatc ctatacaagc catttttatc tgggaaagat 11700
aagaatgagt tgagagagct ggcaaatctt tcttcattgc taagatcagg agaggggtgg 11760
gaagacatac atgtgaaatt cttcaccaag gacatattat tgtgtccaga ggaaatcaga 11820
catgcttgca agttcgggat tgctaaggat aataataaag acatgagcta tcccccttgg 11880
ggaagggaat ccagagggac aattacaaca atccctgttt attatacgac caccccttac 11940
ccaaagatgc tagagatgcc tccaagaatc caaaatcccc tgctgtccgg aatcaggttg 12000
ggccaattac caactggcgc tcattataaa attcggagta tattacatgg aatgggaatc 12060
cattacaggg acttcttgag ttgtggagac ggctccggag ggatgactgc tgcattacta 12120
cgagaaaatg tgcatagcag aggaatattc aatagtctgt tagaattatc agggtcagtc 12180
atgcgaggcg cctctcctga gccccccagt gccctagaaa ctttaggagg agataaatcg 12240
agatgtgtaa atggtgaaac atgttgggaa tatccatctg acttatgtga cccaaggact 12300
tgggactatt tcctccgact caaagcaggc ttggggcttc aaattgattt aattgtaatg 12360
gatatggaag ttcgggattc ttctactagc ctgaaaattg agacgaatgt tagaaattat 12420
gtgcaccgga ttttggatga gcaaggagtt ttaatctaca agacttatgg aacatatatt 12480
tgtgagagcg aaaagaatgc agtaacaatc cttggtccca tgttcaagac ggtcgactta 12540
gttcaaacag aatttagtag ttctcaaacg tctgaagtat atatggtatg taaaggtttg 12600
aagaaattaa tcgatgaacc caatcccgat tggtcttcca tcaatgaatc ctggaaaaac 12660
ctgtacgcat tccagtcatc agaacaggaa tttgccagag caaagaaggt tagtacatac 12720
tttaccttga caggtattcc ctcccaattc attcctgatc cttttgtaaa cattgagact 12780
atgctacaaa tattcggagt acccacgggt gtgtctcatg cggctgcctt aaaatcatct 12840
gatagacctg cagatttatt gaccattagc cttttttata tggcgattat atcgtattat 12900
aacatcaatc atatcagagt aggaccgata cctccgaacc ccccatcaga tggaattgca 12960
caaaatgtgg ggatcgctat aactggtata agcttttggc tgagtttgat ggagaaagac 13020
attccactat atcaacagtg tttagcagtt atccagcaat cattcccgat taggtgggag 13080
gctgtttcag taaaaggagg atacaagcag aagtggagta ctagaggtga tgggctccca 13140
aaagataccc gaacttcaga ctccttggcc ccaatcggga actggatcag atctctggaa 13200
ttggtccgaa accaagttcg tctaaatcca ttcaatgaga tcttgttcaa tcagctatgt 13260
cgtacagtgg ataatcattt gaaatggtca aatttgcgaa gaaacacagg aatgattgaa 13320
tggatcaata gacgaatttc aaaagaagac cggtctatac tgatgttgaa gagtgaccta 13380
cacgaggaaa actcttggag agattaaaaa atcatgagga gactccaaac tttaagtatg 13440
aaaaaaactt tgatccttaa gaccctcttg tggtttttat tttttatctg gttttgtggt 13500
cttcgt 13506
<210> 23
<211> 13503
<212> DNA
<213> 人工序列
<220>
<223> VSV载体: Convac V3 South Africa
<400> 23
acgaagacaa acaaaccatt attatcatta aaaggctcag gagaaacttt aacagtaatc 60
aaaatgtctg ttacagtcaa gagaatcatt gacaacacag tcatagttcc aaaacttcct 120
gcaaatgagg atccagtgga atacccggca gattacttca gaaaatcaaa ggagattcct 180
ctttacatca atactacaaa aagtttgtca gatctaagag gatatgtcta ccaaggcctc 240
aaatccggaa atgtatcaat catacatgtc aacagctact tgtatggagc attaaaggac 300
atccggggta agttggataa agattggtca agtttcggaa taaacatcgg gaaagcaggg 360
gatacaatcg gaatatttga ccttgtatcc ttgaaagccc tggacggcgt acttccagat 420
ggagtatcgg atgcttccag aaccagcgca gatgacaaat ggttgccttt gtatctactt 480
ggcttataca gagtgggcag aacacaaatg cctgaataca gaaaaaagct catggatggg 540
ctgacaaatc aatgcaaaat gatcaatgaa cagtttgaac ctcttgtgcc agaaggtcgt 600
gacatttttg atgtgtgggg aaatgacagt aattacacaa aaattgtcgc tgcagtggac 660
atgttcttcc acatgttcaa aaaacatgaa tgtgcctcgt tcagatacgg aactattgtt 720
tccagattca aagattgtgc tgcattggca acatttggac acctctgcaa aataaccgga 780
atgtctacag aagatgtaac gacctggatc ttgaaccgag aagttgcaga tgaaatggtc 840
caaatgatgc ttccaggcca agaaattgac aaggccgatt catacatgcc ttatttgatc 900
gactttggat tgtcttctaa gtctccatat tcttccgtca aaaaccctgc cttccacttc 960
tgggggcaat tgacagctct tctgctcaga tccaccagag caaggaatgc ccgacagcct 1020
gatgacattg agtatacatc tcttactaca gcaggtttgt tgtacgctta tgcagtagga 1080
tcctctgccg acttggcaca acagttttgt gttggagata acaaatacac tccagatgat 1140
agtaccggag gattgacgac taatgcaccg ccacaaggca gagatgtggt cgaatggctc 1200
ggatggtttg aagatcaaaa cagaaaaccg actcctgata tgatgcagta tgcgaaaaga 1260
gcagtcatgt cactgcaagg cctaagagag aagacaattg gcaagtatgc taagtcagaa 1320
tttgacaaat gaccctataa ttctcagatc acctattata tattatgcta catatgaaaa 1380
aaactaacag atatcatgga taatctcaca aaagttcgtg agtatctcaa gtcctattct 1440
cgtctggatc aggcggtagg agagatagat gagatcgaag cacaacgagc tgaaaagtcc 1500
aattatgagt tgttccaaga ggatggagtg gaagagcata ctaagccctc ttattttcag 1560
gcagcagatg attctgacac agaatctgaa ccagaaattg aagacaatca aggtttgtat 1620
gcaccagatc cagaagctga gcaagttgaa ggctttatac aggggccttt agatgactat 1680
gcagatgagg aagtggatgt tgtatttact tcggactgga aacagcctga gcttgaatct 1740
gacgagcatg gaaagacctt acggttgaca tcgccagagg gtttaagtgg agagcagaaa 1800
tcccagtggc tttcgacgat taaagcagtc gtgcaaagtg ccaaatactg gaatctggca 1860
gagtgcacat ttgaagcatc gggagaaggg gtcattatga aggagcgcca gataactccg 1920
gatgtatata aggtcactcc agtgatgaac acacatccgt cccaatcaga agcagtatca 1980
gatgtttggt ctctctcaaa gacatccatg actttccaac ccaagaaagc aagtcttcag 2040
cctctcacca tatccttgga tgaattgttc tcatctagag gagagttcat ctctgtcgga 2100
ggtgacggac gaatgtctca taaagaggcc atcctgctcg gcctgagata caaaaagttg 2160
tacaatcagg cgagagtcaa atattctctg tagactatga aaaaaagtaa cagatatcac 2220
gatctaagtg ttatcccaat ccattcatca tgagttcctt aaagaagatt ctcggtctga 2280
aggggaaagg taagaaatct aagaaattag ggatcgcacc acccccttat gaagaggaca 2340
ctagcatgga gtatgctccg agcgctccaa ttgacaaatc ctattttgga gttgacgaga 2400
tggacaccta tgatccgaat caattaagat atgagaaatt cttctttaca gtgaaaatga 2460
cggttagatc taatcgtccg ttcagaacat actcagatgt ggcagccgct gtatcccatt 2520
gggatcacat gtacatcgga atggcaggga aacgtccctt ctacaaaatc ttggcttttt 2580
tgggttcttc taatctaaag gccactccag cggtattggc agatcaaggt caaccagagt 2640
atcacactca ctgcgaaggc agggcttatt tgccacatag gatggggaag acccctccca 2700
tgctcaatgt accagagcac ttcagaagac cattcaatat aggtctttac aagggaacga 2760
ttgagctcac aatgaccatc tacgatgatg agtcactgga agcagctcct atgatctggg 2820
atcatttcaa ttcttccaaa ttttctgatt tcagagagaa ggccttaatg tttggcctga 2880
ttgtcgagaa aaaggcatct ggagcgtggg tcctggattc tatcagccac ttcaaatgag 2940
ctagtctaac ttctagcttc tgaacaatcc ccggtttact cagtctctcc taattccagc 3000
ctctcgaaca actaatatcc tgtcttttct atccctatga aaaaaactaa cagagatcga 3060
tctgtttacg cgtcactatg aagtgccttt tgtacttagc ctttttattc attggggtga 3120
attgcaagtt caccatagtt tttccacaca accaaaaagg aaactggaaa aatgttcctt 3180
ctaattacca ttattgcccg tcaagctcag atttaaattg gcataatgac ttaataggca 3240
cagccataca agtcaaaatg cccaagagtc acaaggctat tcaagcagac ggttggatgt 3300
gtcatgcttc caaatgggtc actacttgtg atttccgctg gtatggaccg aagtatataa 3360
cacagtccat ccgatccttc actccatctg tagaacaatg caaggaaagc attgaacaaa 3420
cgaaacaagg aacttggctg aatccaggct tccctcctca aagttgtgga tatgcaactg 3480
tgacggatgc cgaagcagtg attgtccagg tgactcctca ccatgtgctg gttgatgaat 3540
acacaggaga atgggttgat tcacagttca tcaacggaaa atgcagcaat tacatatgcc 3600
ccactgtcca taactctaca acctggcatt ctgactataa ggtcaaaggg ctatgtgatt 3660
ctaacctcat ttccatggac atcaccttct tctcagagga cggagagcta tcatccctgg 3720
gaaaggaggg cacagggttc agaagtaact actttgctta tgaaactgga ggcaaggcct 3780
gcaaaatgca atactgcaag cattggggag tcagactccc atcaggtgtc tggttcgaga 3840
tggctgataa ggatctcttt gctgcagcca gattccctga atgcccagaa gggtcaagta 3900
tctctgctcc atctcagacc tcagtggatg taagtctaat tcaggacgtt gagaggatct 3960
tggattattc cctctgccaa gaaacctgga gcaaaatcag agcgggtctt ccaatctctc 4020
cagtggatct cagctatctt gctcctaaaa acccaggaac cggtcctgct ttcaccataa 4080
tcaatggtac cctaaaatac tttgagacca gatacatcag agtcgatatt gctgctccaa 4140
tcctctcaag aatggtcgga atgatcagtg gaactaccac agaaagggaa ctgtgggatg 4200
actgggcacc atatgaagac gtggaaattg gacccaatgg agttctgagg accagttcag 4260
gatataagtt tcctttatac atgattggac atggtatgtt ggactccgat cttcatctta 4320
gctcaaaggc tcaggtgttc gaacatcctc acattcaaga cgctgcttcg caacttcctg 4380
atgatgagag tttatttttt ggtgatactg ggctatccaa aaatccaatc gagcttgtag 4440
aaggttggtt cagtagttgg aaaagctcta ttgcctcttt tttctttatc atagggttaa 4500
tcattggact attcttggtt ctccgagttg gtatccatct ttgcattaaa ttaaagcaca 4560
ccaagaaaag acagatttat acagacatag agatgaaccg acttggaaag taactcaaat 4620
cctgctaggt atgaaaaaaa ctaacagata tcacgctcga ggccaccatg ttcgtgtttc 4680
tggtgctgct gcctctggtg agctcccagt gcgtgaactt caccacaagg acccagctgc 4740
cccctgccta taccaattcc ttcacacggg gcgtgtacta tcccgacaag gtgttccgga 4800
gcagcgtgct gcactccaca caggatctgt ttctgccttt cttttctaac gtgacctggt 4860
tccacgccat ccacgtgagc ggcaccaatg gcacaaagcg gttcgccaat ccagtgctgc 4920
cctttaacga tggcgtgtac ttcgcctcca ccgagaagtc taacatcatc agaggctgga 4980
tctttggcac cacactggac agcaagacac agtccctgct gatcgtgaac aatgccacca 5040
acgtggtcat caaggtgtgc gagttccagt tttgtaatga tccattcctg ggcgtgtact 5100
atcacaagaa caataagtct tggatggaga gcgagtttcg cgtgtattcc tctgccaaca 5160
attgcacatt tgagtacgtg tcccagccct tcctgatgga cctggagggc aagcagggca 5220
atttcaagaa cctgagggag ttcgtgttta agaatatcga tggctacttc aaaatctact 5280
ccaagcacac cccaatcaac ctggtgcgcg gcctgccaca gggcttctct gccctggagc 5340
cactggtgga tctgcccatc ggcatcaaca tcacccggtt tcagacactg ctggccctgc 5400
acagaagcta cctgacacca ggcgacagct cctctggatg gaccgcagga gcagcagcct 5460
actatgtggg ctatctgcag cccaggacct tcctgctgaa gtacaacgag aatggcacca 5520
tcacagacgc cgtggattgc gccctggatc ccctgtctga gaccaagtgt acactgaaga 5580
gctttaccgt ggagaagggc atctatcaga caagcaattt cagggtgcag cctaccgagt 5640
ccatcgtgcg ctttcccaat atcacaaacc tgtgcccttt tggcgaggtg ttcaacgcaa 5700
cccgcttcgc cagcgtgtac gcctggaata ggaagcgcat ctccaactgc gtggccgact 5760
attctgtgct gtacaacagc gcctccttct ctacctttaa gtgctatggc gtgagcccca 5820
caaagctgaa tgacctgtgc tttaccaacg tgtacgccga ttccttcgtg atcaggggcg 5880
acgaggtgcg ccagatcgca ccaggacaga caggcaatat cgcagactac aattataagc 5940
tgcctgacga tttcaccggc tgcgtgatcg cctggaactc taacaatctg gatagcaaag 6000
tgggcggcaa ctacaattat ctgtaccggc tgtttagaaa gtctaatctg aagccattcg 6060
agagggacat ctccacagaa atctaccagg ccggctctac cccctgcaat ggcgtgaagg 6120
gctttaactg ttatttccct ctgcagagct acggcttcca gccaacatat ggcgtgggct 6180
atcagcccta ccgcgtggtg gtgctgtctt ttgagctgct gcacgcacct gcaacagtgt 6240
gcggaccaaa gaagagcacc aatctggtga agaacaagtg cgtgaacttc aacttcaacg 6300
gactgaccgg aacaggcgtg ctgaccgagt ccaacaagaa gttcctgcct tttcagcagt 6360
tcggcaggga catcgcagat accacagacg ccgtgcgcga ccctcagacc ctggagatcc 6420
tggacatcac accatgctcc ttcggcggcg tgtctgtgat cacaccaggc accaatacaa 6480
gcaaccaggt ggccgtgctg tatcagggcg tgaattgtac cgaggtgcca gtggcaatcc 6540
acgcagatca gctgacccct acatggcggg tgtactctac cggcagcaac gtgttccaga 6600
caagagccgg atgcctgatc ggagcagagc acgtgaacaa tagctatgag tgcgacatcc 6660
ctatcggcgc cggcatctgt gcctcctacc agacccagac aaactcccca aggggatccg 6720
gctacatccc cgaggccccc agagacggcc aggcctacgt gcggaaggac ggcgagtggg 6780
tactgctcag caccttcctg ggcagcagtt ggaaaagctc catcgcctcc tttttcttta 6840
tcatcggcct gatcatcgga ctgttcctgg tgctccgcgt gggtatccac ctgtgcatca 6900
agctgaagca caccaagaaa agacagattt atacagacat cgagatgaac cgacttggaa 6960
agtaagctag ccagattctt catgtttgga ccaaatcaac ttgtgatacc atgctcaaag 7020
aggcctcaat tatatttgag tttttaattt ttatgaaaaa aactaacagc aatcatggaa 7080
gtccacgatt ttgagaccga cgagttcaat gatttcaatg aagatgacta tgccacaaga 7140
gaattcctga atcccgatga gcgcatgacg tacttgaatc atgctgatta caatttgaat 7200
tctcctctaa ttagtgatga tattgacaat ttgatcagga aattcaattc tcttccgatt 7260
ccctcgatgt gggatagtaa gaactgggat ggagttcttg agatgttaac atcatgtcaa 7320
gccaatccca tctcaacatc tcagatgcat aaatggatgg gaagttggtt aatgtctgat 7380
aatcatgatg ccagtcaagg gtatagtttt ttacatgaag tggacaaaga ggcagaaata 7440
acatttgacg tggtggagac cttcatccgc ggctggggca acaaaccaat tgaatacatc 7500
aaaaaggaaa gatggactga ctcattcaaa attctcgctt atttgtgtca aaagtttttg 7560
gacttacaca agttgacatt aatcttaaat gctgtctctg aggtggaatt gctcaacttg 7620
gcgaggactt tcaaaggcaa agtcagaaga agttctcatg gaacgaacat atgcaggatt 7680
agggttccca gcttgggtcc tacttttatt tcagaaggat gggcttactt caagaaactt 7740
gatattctaa tggaccgaaa ctttctgtta atggtcaaag atgtgattat agggaggatg 7800
caaacggtgc tatccatggt atgtagaata gacaacctgt tctcagagca agacatcttc 7860
tcccttctaa atatctacag aattggagat aaaattgtgg agaggcaggg aaatttttct 7920
tatgacttga ttaaaatggt ggaaccgata tgcaacttga agctgatgaa attagcaaga 7980
gaatcaaggc ctttagtccc acaattccct cattttgaaa atcatatcaa gacttctgtt 8040
gatgaagggg caaaaattga ccgaggtata agattcctcc atgatcagat aatgagtgtg 8100
aaaacagtgg atctcacact ggtgatttat ggatcgttca gacattgggg tcatcctttt 8160
atagattatt acactggact agaaaaatta cattcccaag taaccatgaa gaaagatatt 8220
gatgtgtcat atgcaaaagc acttgcaagt gatttagctc ggattgttct atttcaacag 8280
ttcaatgatc ataaaaagtg gttcgtgaat ggagacttgc tccctcatga tcatcccttt 8340
aaaagtcatg ttaaagaaaa tacatggccc acagctgctc aagttcaaga ttttggagat 8400
aaatggcatg aacttccgct gattaaatgt tttgaaatac ccgacttact agacccatcg 8460
ataatatact ctgacaaaag tcattcaatg aataggtcag aggtgttgaa acatgtccga 8520
atgaatccga acactcctat ccctagtaaa aaggtgttgc agactatgtt ggacacaaag 8580
gctaccaatt ggaaagaatt tcttaaagag attgatgaga agggcttaga tgatgatgat 8640
ctaattattg gtcttaaagg aaaggagagg gaactgaagt tggcaggtag atttttctcc 8700
ctaatgtctt ggaaattgcg agaatacttt gtaattaccg aatatttgat aaagactcat 8760
ttcgtcccta tgtttaaagg cctgacaatg gcggacgatc taactgcagt cattaaaaag 8820
atgttagatt cctcatccgg ccaaggattg aagtcatatg aggcaatttg catagccaat 8880
cacattgatt acgaaaaatg gaataaccac caaaggaagt tatcaaacgg cccagtgttc 8940
cgagttatgg gccagttctt aggttatcca tccttaatcg agagaactca tgaatttttt 9000
gagaaaagtc ttatatacta caatggaaga ccagacttga tgcgtgttca caacaacaca 9060
ctgatcaatt caacctccca acgagtttgt tggcaaggac aagagggtgg actggaaggt 9120
ctacggcaaa aaggatggac tatcctcaat ctactggtta ttcaaagaga ggctaaaatc 9180
agaaacactg ctgtcaaagt cttggcacaa ggtgataatc aagttatttg cacacagtat 9240
aaaacgaaga aatcgagaaa cgttgtagaa ttacagggtg ctctcaatca aatggtttct 9300
aataatgaga aaattatgac tgcaatcaaa atagggacag ggaagttagg acttttgata 9360
aatgacgatg agactatgca atctgcagat tacttgaatt atggaaaaat accgattttc 9420
cgtggagtga ttagagggtt agagaccaag agatggtcac gagtgacttg tgtcaccaat 9480
gaccaaatac ccacttgtgc taatataatg agctcagttt ccacaaatgc tctcaccgta 9540
gctcattttg ctgagaaccc aatcaatgcc atgatacagt acaattattt tgggacattt 9600
gctagactct tgttgatgat gcatgatcct gctcttcgtc aatcattgta tgaagttcaa 9660
gataagatac cgggcttgca cagttctact ttcaaatacg ccatgttgta tttggaccct 9720
tccattggag gagtgtcggg catgtctttg tccaggtttt tgattagagc cttcccagat 9780
cccgtaacag aaagtctctc attctggaga ttcatccatg tacatgctcg aagtgagcat 9840
ctgaaggaga tgagtgcagt atttggaaac cccgagatag ccaagtttcg aataactcac 9900
atagacaagc tagtagaaga tccaacctct ctgaacatcg ctatgggaat gagtccagcg 9960
aacttgttaa agactgaggt taaaaaatgc ttaatcgaat caagacaaac catcaggaac 10020
caggtgatta aggatgcaac catatatttg tatcatgaag aggatcggct cagaagtttc 10080
ttatggtcaa taaatcctct gttccctaga tttttaagtg aattcaaatc aggcactttt 10140
ttgggagtcg cagacgggct catcagtcta tttcaaaatt ctcgtactat tcggaactcc 10200
tttaagaaaa agtatcatag ggaattggat gatttgattg tgaggagtga ggtatcctct 10260
ttgacacatt tagggaaact tcatttgaga aggggatcat gtaaaatgtg gacatgttca 10320
gctactcatg ctgacacatt aagatacaaa tcctggggcc gtacagttat tgggacaact 10380
gtaccccatc cattagaaat gttgggtcca caacatcgaa aagagactcc ttgtgcacca 10440
tgtaacacat cagggttcaa ttatgtttct gtgcattgtc cagacgggat ccatgacgtc 10500
tttagttcac ggggaccatt gcctgcttat ctagggtcta aaacatctga atctacatct 10560
attttgcagc cttgggaaag ggaaagcaaa gtcccactga ttaaaagagc tacacgtctt 10620
agagatgcta tctcttggtt tgttgaaccc gactctaaac tagcaatgac tatactttct 10680
aacatccact ctttaacagg cgaagaatgg accaaaaggc agcatgggtt caaaagaaca 10740
gggtctgccc ttcataggtt ttcgacatct cggatgagcc atggtgggtt cgcatctcag 10800
agcactgcag cattgaccag gttgatggca actacagaca ccatgaggga tctgggagat 10860
cagaatttcg actttttatt ccaagcaacg ttgctctatg ctcaaattac caccactgtt 10920
gcaagagacg gatggatcac cagttgtaca gatcattatc atattgcctg taagtcctgt 10980
ttgagaccca tagaagagat caccctggac tcaagtatgg actacacgcc cccagatgta 11040
tcccatgtgc tgaagacatg gaggaatggg gaaggttcgt ggggacaaga gataaaacag 11100
atctatcctt tagaagggaa ttggaagaat ttagcacctg ctgagcaatc ctatcaagtc 11160
ggcagatgta taggttttct atatggagac ttggcgtata gaaaatctac tcatgccgag 11220
gacagttctc tatttcctct atctatacaa ggtcgtatta gaggtcgagg tttcttaaaa 11280
gggttgctag acggattaat gagagcaagt tgctgccaag taatacaccg gagaagtctg 11340
gctcatttga agaggccggc caacgcagtg tacggaggtt tgatttactt gattgataaa 11400
ttgagtgtat cacctccatt cctttctctt actagatcag gacctattag agacgaatta 11460
gaaacgattc cccacaagat cccaacctcc tatccgacaa gcaaccgtga tatgggggtg 11520
attgtcagaa attacttcaa ataccaatgc cgtctaattg aaaagggaaa atacagatca 11580
cattattcac aattatggtt attctcagat gtcttatcca tagacttcat tggaccattc 11640
tctatttcca ccaccctctt gcaaatccta tacaagccat ttttatctgg gaaagataag 11700
aatgagttga gagagctggc aaatctttct tcattgctaa gatcaggaga ggggtgggaa 11760
gacatacatg tgaaattctt caccaaggac atattattgt gtccagagga aatcagacat 11820
gcttgcaagt tcgggattgc taaggataat aataaagaca tgagctatcc cccttgggga 11880
agggaatcca gagggacaat tacaacaatc cctgtttatt atacgaccac cccttaccca 11940
aagatgctag agatgcctcc aagaatccaa aatcccctgc tgtccggaat caggttgggc 12000
caattaccaa ctggcgctca ttataaaatt cggagtatat tacatggaat gggaatccat 12060
tacagggact tcttgagttg tggagacggc tccggaggga tgactgctgc attactacga 12120
gaaaatgtgc atagcagagg aatattcaat agtctgttag aattatcagg gtcagtcatg 12180
cgaggcgcct ctcctgagcc ccccagtgcc ctagaaactt taggaggaga taaatcgaga 12240
tgtgtaaatg gtgaaacatg ttgggaatat ccatctgact tatgtgaccc aaggacttgg 12300
gactatttcc tccgactcaa agcaggcttg gggcttcaaa ttgatttaat tgtaatggat 12360
atggaagttc gggattcttc tactagcctg aaaattgaga cgaatgttag aaattatgtg 12420
caccggattt tggatgagca aggagtttta atctacaaga cttatggaac atatatttgt 12480
gagagcgaaa agaatgcagt aacaatcctt ggtcccatgt tcaagacggt cgacttagtt 12540
caaacagaat ttagtagttc tcaaacgtct gaagtatata tggtatgtaa aggtttgaag 12600
aaattaatcg atgaacccaa tcccgattgg tcttccatca atgaatcctg gaaaaacctg 12660
tacgcattcc agtcatcaga acaggaattt gccagagcaa agaaggttag tacatacttt 12720
accttgacag gtattccctc ccaattcatt cctgatcctt ttgtaaacat tgagactatg 12780
ctacaaatat tcggagtacc cacgggtgtg tctcatgcgg ctgccttaaa atcatctgat 12840
agacctgcag atttattgac cattagcctt ttttatatgg cgattatatc gtattataac 12900
atcaatcata tcagagtagg accgatacct ccgaaccccc catcagatgg aattgcacaa 12960
aatgtgggga tcgctataac tggtataagc ttttggctga gtttgatgga gaaagacatt 13020
ccactatatc aacagtgttt agcagttatc cagcaatcat tcccgattag gtgggaggct 13080
gtttcagtaa aaggaggata caagcagaag tggagtacta gaggtgatgg gctcccaaaa 13140
gatacccgaa cttcagactc cttggcccca atcgggaact ggatcagatc tctggaattg 13200
gtccgaaacc aagttcgtct aaatccattc aatgagatct tgttcaatca gctatgtcgt 13260
acagtggata atcatttgaa atggtcaaat ttgcgaagaa acacaggaat gattgaatgg 13320
atcaatagac gaatttcaaa agaagaccgg tctatactga tgttgaagag tgacctacac 13380
gaggaaaact cttggagaga ttaaaaaatc atgaggagac tccaaacttt aagtatgaaa 13440
aaaactttga tccttaagac cctcttgtgg tttttatttt ttatctggtt ttgtggtctt 13500
cgt 13503
<210> 24
<211> 13484
<212> DNA
<213> 人工序列
<220>
<223> VSV载体: Convac V4 China
<400> 24
acgaagacaa acaaaccatt attatcatta aaaggctcag gagaaacttt aacagtaatc 60
aaaatgtctg ttacagtcaa gagaatcatt gacaacacag tcatagttcc aaaacttcct 120
gcaaatgagg atccagtgga atacccggca gattacttca gaaaatcaaa ggagattcct 180
ctttacatca atactacaaa aagtttgtca gatctaagag gatatgtcta ccaaggcctc 240
aaatccggaa atgtatcaat catacatgtc aacagctact tgtatggagc attaaaggac 300
atccggggta agttggataa agattggtca agtttcggaa taaacatcgg gaaagcaggg 360
gatacaatcg gaatatttga ccttgtatcc ttgaaagccc tggacggcgt acttccagat 420
ggagtatcgg atgcttccag aaccagcgca gatgacaaat ggttgccttt gtatctactt 480
ggcttataca gagtgggcag aacacaaatg cctgaataca gaaaaaagct catggatggg 540
ctgacaaatc aatgcaaaat gatcaatgaa cagtttgaac ctcttgtgcc agaaggtcgt 600
gacatttttg atgtgtgggg aaatgacagt aattacacaa aaattgtcgc tgcagtggac 660
atgttcttcc acatgttcaa aaaacatgaa tgtgcctcgt tcagatacgg aactattgtt 720
tccagattca aagattgtgc tgcattggca acatttggac acctctgcaa aataaccgga 780
atgtctacag aagatgtaac gacctggatc ttgaaccgag aagttgcaga tgaaatggtc 840
caaatgatgc ttccaggcca agaaattgac aaggccgatt catacatgcc ttatttgatc 900
gactttggat tgtcttctaa gtctccatat tcttccgtca aaaaccctgc cttccacttc 960
tgggggcaat tgacagctct tctgctcaga tccaccagag caaggaatgc ccgacagcct 1020
gatgacattg agtatacatc tcttactaca gcaggtttgt tgtacgctta tgcagtagga 1080
tcctctgccg acttggcaca acagttttgt gttggagata acaaatacac tccagatgat 1140
agtaccggag gattgacgac taatgcaccg ccacaaggca gagatgtggt cgaatggctc 1200
ggatggtttg aagatcaaaa cagaaaaccg actcctgata tgatgcagta tgcgaaaaga 1260
gcagtcatgt cactgcaagg cctaagagag aagacaattg gcaagtatgc taagtcagaa 1320
tttgacaaat gaccctataa ttctcagatc acctattata tattatgcta catatgaaaa 1380
aaactaacag atatcatgga taatctcaca aaagttcgtg agtatctcaa gtcctattct 1440
cgtctggatc aggcggtagg agagatagat gagatcgaag cacaacgagc tgaaaagtcc 1500
aattatgagt tgttccaaga ggatggagtg gaagagcata ctaagccctc ttattttcag 1560
gcagcagatg attctgacac agaatctgaa ccagaaattg aagacaatca aggtttgtat 1620
gcaccagatc cagaagctga gcaagttgaa ggctttatac aggggccttt agatgactat 1680
gcagatgagg aagtggatgt tgtatttact tcggactgga aacagcctga gcttgaatct 1740
gacgagcatg gaaagacctt acggttgaca tcgccagagg gtttaagtgg agagcagaaa 1800
tcccagtggc tttcgacgat taaagcagtc gtgcaaagtg ccaaatactg gaatctggca 1860
gagtgcacat ttgaagcatc gggagaaggg gtcattatga aggagcgcca gataactccg 1920
gatgtatata aggtcactcc agtgatgaac acacatccgt cccaatcaga agcagtatca 1980
gatgtttggt ctctctcaaa gacatccatg actttccaac ccaagaaagc aagtcttcag 2040
cctctcacca tatccttgga tgaattgttc tcatctagag gagagttcat ctctgtcgga 2100
ggtgacggac gaatgtctca taaagaggcc atcctgctcg gcctgagata caaaaagttg 2160
tacaatcagg cgagagtcaa atattctctg tagactatga aaaaaagtaa cagatatcac 2220
gatctaagtg ttatcccaat ccattcatca tgagttcctt aaagaagatt ctcggtctga 2280
aggggaaagg taagaaatct aagaaattag ggatcgcacc acccccttat gaagaggaca 2340
ctagcatgga gtatgctccg agcgctccaa ttgacaaatc ctattttgga gttgacgaga 2400
tggacaccta tgatccgaat caattaagat atgagaaatt cttctttaca gtgaaaatga 2460
cggttagatc taatcgtccg ttcagaacat actcagatgt ggcagccgct gtatcccatt 2520
gggatcacat gtacatcgga atggcaggga aacgtccctt ctacaaaatc ttggcttttt 2580
tgggttcttc taatctaaag gccactccag cggtattggc agatcaaggt caaccagagt 2640
atcacactca ctgcgaaggc agggcttatt tgccacatag gatggggaag acccctccca 2700
tgctcaatgt accagagcac ttcagaagac cattcaatat aggtctttac aagggaacga 2760
ttgagctcac aatgaccatc tacgatgatg agtcactgga agcagctcct atgatctggg 2820
atcatttcaa ttcttccaaa ttttctgatt tcagagagaa ggccttaatg tttggcctga 2880
ttgtcgagaa aaaggcatct ggagcgtggg tcctggattc tatcagccac ttcaaatgag 2940
ctagtctaac ttctagcttc tgaacaatcc ccggtttact cagtctctcc taattccagc 3000
ctctcgaaca actaatatcc tgtcttttct atccctatga aaaaaactaa cagagatcga 3060
tctgtttacg cgtgccacca tgttcgtgtt tctggtgctg ctgcctctgg tgagctccca 3120
gtgcgtgaac ctgaccacaa ggacccagct gccccctgcc tataccaatt ccttcacacg 3180
gggcgtgtac tatcccgaca aggtgttccg gagcagcgtg ctgcactcca cacaggatct 3240
gtttctgcct ttcttttcta acgtgacctg gttccacgcc atccacgtga gcggcaccaa 3300
tggcacaaag cggttcgaca atccagtgct gccctttaac gatggcgtgt acttcgcctc 3360
caccgagaag tctaacatca tcagaggctg gatctttggc accacactgg acagcaagac 3420
acagtccctg ctgatcgtga acaatgccac caacgtggtc atcaaggtgt gcgagttcca 3480
gttttgtaat gatccattcc tgggcgtgta ctatcacaag aacaataagt cttggatgga 3540
gagcgagttt cgcgtgtatt cctctgccaa caattgcaca tttgagtacg tgtcccagcc 3600
cttcctgatg gacctggagg gcaagcaggg caatttcaag aacctgaggg agttcgtgtt 3660
taagaatatc gatggctact tcaaaatcta ctccaagcac accccaatca acctggtgcg 3720
cgacctgcca cagggcttct ctgccctgga gccactggtg gatctgccca tcggcatcaa 3780
catcacccgg tttcagacac tgctggccct gcacagaagc tacctgacac caggcgacag 3840
ctcctctgga tggaccgcag gagcagcagc ctactatgtg ggctatctgc agcccaggac 3900
cttcctgctg aagtacaacg agaatggcac catcacagac gccgtggatt gcgccctgga 3960
tcccctgtct gagaccaagt gtacactgaa gagctttacc gtggagaagg gcatctatca 4020
gacaagcaat ttcagggtgc agcctaccga gtccatcgtg cgctttccca atatcacaaa 4080
cctgtgccct tttggcgagg tgttcaacgc aacccgcttc gccagcgtgt acgcctggaa 4140
taggaagcgc atctccaact gcgtggccga ctattctgtg ctgtacaaca gcgcctcctt 4200
ctctaccttt aagtgctatg gcgtgagccc cacaaagctg aatgacctgt gctttaccaa 4260
cgtgtacgcc gattccttcg tgatcagggg cgacgaggtg cgccagatcg caccaggaca 4320
gacaggcaag atcgcagact acaattataa gctgcctgac gatttcaccg gctgcgtgat 4380
cgcctggaac tctaacaatc tggatagcaa agtgggcggc aactacaatt atctgtaccg 4440
gctgtttaga aagtctaatc tgaagccatt cgagagggac atctccacag aaatctacca 4500
ggccggctct accccctgca atggcgtgga gggctttaac tgttatttcc ctctgcagag 4560
ctacggcttc cagccaacaa acggcgtggg ctatcagccc taccgcgtgg tggtgctgtc 4620
ttttgagctg ctgcacgcac ctgcaacagt gtgcggacca aagaagagca ccaatctggt 4680
gaagaacaag tgcgtgaact tcaacttcaa cggactgacc ggaacaggcg tgctgaccga 4740
gtccaacaag aagttcctgc cttttcagca gttcggcagg gacatcgcag ataccacaga 4800
cgccgtgcgc gaccctcaga ccctggagat cctggacatc acaccatgct ccttcggcgg 4860
cgtgtctgtg atcacaccag gcaccaatac aagcaaccag gtggccgtgc tgtatcagga 4920
cgtgaattgt accgaggtgc cagtggcaat ccacgcagat cagctgaccc ctacatggcg 4980
ggtgtactct accggcagca acgtgttcca gacaagagcc ggatgcctga tcggagcaga 5040
gcacgtgaac aatagctatg agtgcgacat ccctatcggc gccggcatct gtgcctccta 5100
ccagacccag acaaactccc caaggtctgt gggcgataca ggcctgtcca agaatccaat 5160
cgagctggta gagggctggt tcagcagttg gaaaagctcc atcgcctcct ttttctttat 5220
catcggcctg atcatcggac tgttcctggt gctccgcgtg ggtatccacc tgtgcatcaa 5280
gctgaagcac accaagaaaa gacagattta tacagacatc gagatgaacc gcctgggaaa 5340
gggatccggc tccggcgagg gcaggggaag tctactaaca tgcggggacg tggaggaaaa 5400
tcccggcccc atgaagtgcc ttttgtactt agccttttta ttcattgggg tgaattgcaa 5460
gttcaccata gtttttccac acaaccaaaa aggaaactgg aaaaatgttc cttctaatta 5520
ccattattgc ccgtcaagct cagatttaaa ttggcataat gacttaatag gcacagccat 5580
acaagtcaaa atgcccaaga gtcacaaggc tattcaagca gacggttgga tgtgtcatgc 5640
ttccaaatgg gtcactactt gtgatttccg ctggtatgga ccgaagtata taacacagtc 5700
catccgatcc ttcactccat ctgtagaaca atgcaaggaa agcattgaac aaacgaaaca 5760
aggaacttgg ctgaatccag gcttccctcc tcaaagttgt ggatatgcaa ctgtgacgga 5820
tgccgaagca gtgattgtcc aggtgactcc tcaccatgtg ctggttgatg aatacacagg 5880
agaatgggtt gattcacagt tcatcaacgg aaaatgcagc aattacatat gccccactgt 5940
ccataactct acaacctggc attctgacta taaggtcaaa gggctatgtg attctaacct 6000
catttccatg gacatcacct tcttctcaga ggacggagag ctatcatccc tgggaaagga 6060
gggcacaggg ttcagaagta actactttgc ttatgaaact ggaggcaagg cctgcaaaat 6120
gcaatactgc aagcattggg gagtcagact cccatcaggt gtctggttcg agatggctga 6180
taaggatctc tttgctgcag ccagattccc tgaatgccca gaagggtcaa gtatctctgc 6240
tccatctcag acctcagtgg atgtaagtct aattcaggac gttgagagga tcttggatta 6300
ttccctctgc caagaaacct ggagcaaaat cagagcgggt cttccaatct ctccagtgga 6360
tctcagctat cttgctccta aaaacccagg aaccggtcct gctttcacca taatcaatgg 6420
taccctaaaa tactttgaga ccagatacat cagagtcgat attgctgctc caatcctctc 6480
aagaatggtc ggaatgatca gtggaactac cacagaaagg gaactgtggg atgactgggc 6540
accatatgaa gacgtggaaa ttggacccaa tggagttctg aggaccagtt caggatataa 6600
gtttccttta tacatgattg gacatggtat gttggactcc gatcttcatc ttagctcaaa 6660
ggctcaggtg ttcgaacatc ctcacattca agacgctgct tcgcaacttc ctgatgatga 6720
gagtttattt tttggtgata ctgggctatc caaaaatcca atcgagcttg tagaaggttg 6780
gttcagtagt tggaaaagct ctattgcctc ttttttcttt atcatagggt taatcattgg 6840
actattcttg gttctccgag ttggtatcca tctttgcatt aaattaaagc acaccaagaa 6900
aagacagatt tatacagaca tagagatgaa ccgacttgga aagtaagcta gccagattct 6960
tcatgtttgg accaaatcaa cttgtgatac catgctcaaa gaggcctcaa ttatatttga 7020
gtttttaatt tttatgaaaa aaactaacag caatcatgga agtccacgat tttgagaccg 7080
acgagttcaa tgatttcaat gaagatgact atgccacaag agaattcctg aatcccgatg 7140
agcgcatgac gtacttgaat catgctgatt acaatttgaa ttctcctcta attagtgatg 7200
atattgacaa tttgatcagg aaattcaatt ctcttccgat tccctcgatg tgggatagta 7260
agaactggga tggagttctt gagatgttaa catcatgtca agccaatccc atctcaacat 7320
ctcagatgca taaatggatg ggaagttggt taatgtctga taatcatgat gccagtcaag 7380
ggtatagttt tttacatgaa gtggacaaag aggcagaaat aacatttgac gtggtggaga 7440
ccttcatccg cggctggggc aacaaaccaa ttgaatacat caaaaaggaa agatggactg 7500
actcattcaa aattctcgct tatttgtgtc aaaagttttt ggacttacac aagttgacat 7560
taatcttaaa tgctgtctct gaggtggaat tgctcaactt ggcgaggact ttcaaaggca 7620
aagtcagaag aagttctcat ggaacgaaca tatgcaggat tagggttccc agcttgggtc 7680
ctacttttat ttcagaagga tgggcttact tcaagaaact tgatattcta atggaccgaa 7740
actttctgtt aatggtcaaa gatgtgatta tagggaggat gcaaacggtg ctatccatgg 7800
tatgtagaat agacaacctg ttctcagagc aagacatctt ctcccttcta aatatctaca 7860
gaattggaga taaaattgtg gagaggcagg gaaatttttc ttatgacttg attaaaatgg 7920
tggaaccgat atgcaacttg aagctgatga aattagcaag agaatcaagg cctttagtcc 7980
cacaattccc tcattttgaa aatcatatca agacttctgt tgatgaaggg gcaaaaattg 8040
accgaggtat aagattcctc catgatcaga taatgagtgt gaaaacagtg gatctcacac 8100
tggtgattta tggatcgttc agacattggg gtcatccttt tatagattat tacactggac 8160
tagaaaaatt acattcccaa gtaaccatga agaaagatat tgatgtgtca tatgcaaaag 8220
cacttgcaag tgatttagct cggattgttc tatttcaaca gttcaatgat cataaaaagt 8280
ggttcgtgaa tggagacttg ctccctcatg atcatccctt taaaagtcat gttaaagaaa 8340
atacatggcc cacagctgct caagttcaag attttggaga taaatggcat gaacttccgc 8400
tgattaaatg ttttgaaata cccgacttac tagacccatc gataatatac tctgacaaaa 8460
gtcattcaat gaataggtca gaggtgttga aacatgtccg aatgaatccg aacactccta 8520
tccctagtaa aaaggtgttg cagactatgt tggacacaaa ggctaccaat tggaaagaat 8580
ttcttaaaga gattgatgag aagggcttag atgatgatga tctaattatt ggtcttaaag 8640
gaaaggagag ggaactgaag ttggcaggta gatttttctc cctaatgtct tggaaattgc 8700
gagaatactt tgtaattacc gaatatttga taaagactca tttcgtccct atgtttaaag 8760
gcctgacaat ggcggacgat ctaactgcag tcattaaaaa gatgttagat tcctcatccg 8820
gccaaggatt gaagtcatat gaggcaattt gcatagccaa tcacattgat tacgaaaaat 8880
ggaataacca ccaaaggaag ttatcaaacg gcccagtgtt ccgagttatg ggccagttct 8940
taggttatcc atccttaatc gagagaactc atgaattttt tgagaaaagt cttatatact 9000
acaatggaag accagacttg atgcgtgttc acaacaacac actgatcaat tcaacctccc 9060
aacgagtttg ttggcaagga caagagggtg gactggaagg tctacggcaa aaaggatgga 9120
ctatcctcaa tctactggtt attcaaagag aggctaaaat cagaaacact gctgtcaaag 9180
tcttggcaca aggtgataat caagttattt gcacacagta taaaacgaag aaatcgagaa 9240
acgttgtaga attacagggt gctctcaatc aaatggtttc taataatgag aaaattatga 9300
ctgcaatcaa aatagggaca gggaagttag gacttttgat aaatgacgat gagactatgc 9360
aatctgcaga ttacttgaat tatggaaaaa taccgatttt ccgtggagtg attagagggt 9420
tagagaccaa gagatggtca cgagtgactt gtgtcaccaa tgaccaaata cccacttgtg 9480
ctaatataat gagctcagtt tccacaaatg ctctcaccgt agctcatttt gctgagaacc 9540
caatcaatgc catgatacag tacaattatt ttgggacatt tgctagactc ttgttgatga 9600
tgcatgatcc tgctcttcgt caatcattgt atgaagttca agataagata ccgggcttgc 9660
acagttctac tttcaaatac gccatgttgt atttggaccc ttccattgga ggagtgtcgg 9720
gcatgtcttt gtccaggttt ttgattagag ccttcccaga tcccgtaaca gaaagtctct 9780
cattctggag attcatccat gtacatgctc gaagtgagca tctgaaggag atgagtgcag 9840
tatttggaaa ccccgagata gccaagtttc gaataactca catagacaag ctagtagaag 9900
atccaacctc tctgaacatc gctatgggaa tgagtccagc gaacttgtta aagactgagg 9960
ttaaaaaatg cttaatcgaa tcaagacaaa ccatcaggaa ccaggtgatt aaggatgcaa 10020
ccatatattt gtatcatgaa gaggatcggc tcagaagttt cttatggtca ataaatcctc 10080
tgttccctag atttttaagt gaattcaaat caggcacttt tttgggagtc gcagacgggc 10140
tcatcagtct atttcaaaat tctcgtacta ttcggaactc ctttaagaaa aagtatcata 10200
gggaattgga tgatttgatt gtgaggagtg aggtatcctc tttgacacat ttagggaaac 10260
ttcatttgag aaggggatca tgtaaaatgt ggacatgttc agctactcat gctgacacat 10320
taagatacaa atcctggggc cgtacagtta ttgggacaac tgtaccccat ccattagaaa 10380
tgttgggtcc acaacatcga aaagagactc cttgtgcacc atgtaacaca tcagggttca 10440
attatgtttc tgtgcattgt ccagacggga tccatgacgt ctttagttca cggggaccat 10500
tgcctgctta tctagggtct aaaacatctg aatctacatc tattttgcag ccttgggaaa 10560
gggaaagcaa agtcccactg attaaaagag ctacacgtct tagagatgct atctcttggt 10620
ttgttgaacc cgactctaaa ctagcaatga ctatactttc taacatccac tctttaacag 10680
gcgaagaatg gaccaaaagg cagcatgggt tcaaaagaac agggtctgcc cttcataggt 10740
tttcgacatc tcggatgagc catggtgggt tcgcatctca gagcactgca gcattgacca 10800
ggttgatggc aactacagac accatgaggg atctgggaga tcagaatttc gactttttat 10860
tccaagcaac gttgctctat gctcaaatta ccaccactgt tgcaagagac ggatggatca 10920
ccagttgtac agatcattat catattgcct gtaagtcctg tttgagaccc atagaagaga 10980
tcaccctgga ctcaagtatg gactacacgc ccccagatgt atcccatgtg ctgaagacat 11040
ggaggaatgg ggaaggttcg tggggacaag agataaaaca gatctatcct ttagaaggga 11100
attggaagaa tttagcacct gctgagcaat cctatcaagt cggcagatgt ataggttttc 11160
tatatggaga cttggcgtat agaaaatcta ctcatgccga ggacagttct ctatttcctc 11220
tatctataca aggtcgtatt agaggtcgag gtttcttaaa agggttgcta gacggattaa 11280
tgagagcaag ttgctgccaa gtaatacacc ggagaagtct ggctcatttg aagaggccgg 11340
ccaacgcagt gtacggaggt ttgatttact tgattgataa attgagtgta tcacctccat 11400
tcctttctct tactagatca ggacctatta gagacgaatt agaaacgatt ccccacaaga 11460
tcccaacctc ctatccgaca agcaaccgtg atatgggggt gattgtcaga aattacttca 11520
aataccaatg ccgtctaatt gaaaagggaa aatacagatc acattattca caattatggt 11580
tattctcaga tgtcttatcc atagacttca ttggaccatt ctctatttcc accaccctct 11640
tgcaaatcct atacaagcca tttttatctg ggaaagataa gaatgagttg agagagctgg 11700
caaatctttc ttcattgcta agatcaggag aggggtggga agacatacat gtgaaattct 11760
tcaccaagga catattattg tgtccagagg aaatcagaca tgcttgcaag ttcgggattg 11820
ctaaggataa taataaagac atgagctatc ccccttgggg aagggaatcc agagggacaa 11880
ttacaacaat ccctgtttat tatacgacca ccccttaccc aaagatgcta gagatgcctc 11940
caagaatcca aaatcccctg ctgtccggaa tcaggttggg ccaattacca actggcgctc 12000
attataaaat tcggagtata ttacatggaa tgggaatcca ttacagggac ttcttgagtt 12060
gtggagacgg ctccggaggg atgactgctg cattactacg agaaaatgtg catagcagag 12120
gaatattcaa tagtctgtta gaattatcag ggtcagtcat gcgaggcgcc tctcctgagc 12180
cccccagtgc cctagaaact ttaggaggag ataaatcgag atgtgtaaat ggtgaaacat 12240
gttgggaata tccatctgac ttatgtgacc caaggacttg ggactatttc ctccgactca 12300
aagcaggctt ggggcttcaa attgatttaa ttgtaatgga tatggaagtt cgggattctt 12360
ctactagcct gaaaattgag acgaatgtta gaaattatgt gcaccggatt ttggatgagc 12420
aaggagtttt aatctacaag acttatggaa catatatttg tgagagcgaa aagaatgcag 12480
taacaatcct tggtcccatg ttcaagacgg tcgacttagt tcaaacagaa tttagtagtt 12540
ctcaaacgtc tgaagtatat atggtatgta aaggtttgaa gaaattaatc gatgaaccca 12600
atcccgattg gtcttccatc aatgaatcct ggaaaaacct gtacgcattc cagtcatcag 12660
aacaggaatt tgccagagca aagaaggtta gtacatactt taccttgaca ggtattccct 12720
cccaattcat tcctgatcct tttgtaaaca ttgagactat gctacaaata ttcggagtac 12780
ccacgggtgt gtctcatgcg gctgccttaa aatcatctga tagacctgca gatttattga 12840
ccattagcct tttttatatg gcgattatat cgtattataa catcaatcat atcagagtag 12900
gaccgatacc tccgaacccc ccatcagatg gaattgcaca aaatgtgggg atcgctataa 12960
ctggtataag cttttggctg agtttgatgg agaaagacat tccactatat caacagtgtt 13020
tagcagttat ccagcaatca ttcccgatta ggtgggaggc tgtttcagta aaaggaggat 13080
acaagcagaa gtggagtact agaggtgatg ggctcccaaa agatacccga acttcagact 13140
ccttggcccc aatcgggaac tggatcagat ctctggaatt ggtccgaaac caagttcgtc 13200
taaatccatt caatgagatc ttgttcaatc agctatgtcg tacagtggat aatcatttga 13260
aatggtcaaa tttgcgaaga aacacaggaa tgattgaatg gatcaataga cgaatttcaa 13320
aagaagaccg gtctatactg atgttgaaga gtgacctaca cgaggaaaac tcttggagag 13380
attaaaaaat catgaggaga ctccaaactt taagtatgaa aaaaactttg atccttaaga 13440
ccctcttgtg gtttttattt tttatctggt tttgtggtct tcgt 13484
<210> 25
<211> 13484
<212> DNA
<213> 人工序列
<220>
<223> VSV载体: Convac V4 South Africa
<400> 25
acgaagacaa acaaaccatt attatcatta aaaggctcag gagaaacttt aacagtaatc 60
aaaatgtctg ttacagtcaa gagaatcatt gacaacacag tcatagttcc aaaacttcct 120
gcaaatgagg atccagtgga atacccggca gattacttca gaaaatcaaa ggagattcct 180
ctttacatca atactacaaa aagtttgtca gatctaagag gatatgtcta ccaaggcctc 240
aaatccggaa atgtatcaat catacatgtc aacagctact tgtatggagc attaaaggac 300
atccggggta agttggataa agattggtca agtttcggaa taaacatcgg gaaagcaggg 360
gatacaatcg gaatatttga ccttgtatcc ttgaaagccc tggacggcgt acttccagat 420
ggagtatcgg atgcttccag aaccagcgca gatgacaaat ggttgccttt gtatctactt 480
ggcttataca gagtgggcag aacacaaatg cctgaataca gaaaaaagct catggatggg 540
ctgacaaatc aatgcaaaat gatcaatgaa cagtttgaac ctcttgtgcc agaaggtcgt 600
gacatttttg atgtgtgggg aaatgacagt aattacacaa aaattgtcgc tgcagtggac 660
atgttcttcc acatgttcaa aaaacatgaa tgtgcctcgt tcagatacgg aactattgtt 720
tccagattca aagattgtgc tgcattggca acatttggac acctctgcaa aataaccgga 780
atgtctacag aagatgtaac gacctggatc ttgaaccgag aagttgcaga tgaaatggtc 840
caaatgatgc ttccaggcca agaaattgac aaggccgatt catacatgcc ttatttgatc 900
gactttggat tgtcttctaa gtctccatat tcttccgtca aaaaccctgc cttccacttc 960
tgggggcaat tgacagctct tctgctcaga tccaccagag caaggaatgc ccgacagcct 1020
gatgacattg agtatacatc tcttactaca gcaggtttgt tgtacgctta tgcagtagga 1080
tcctctgccg acttggcaca acagttttgt gttggagata acaaatacac tccagatgat 1140
agtaccggag gattgacgac taatgcaccg ccacaaggca gagatgtggt cgaatggctc 1200
ggatggtttg aagatcaaaa cagaaaaccg actcctgata tgatgcagta tgcgaaaaga 1260
gcagtcatgt cactgcaagg cctaagagag aagacaattg gcaagtatgc taagtcagaa 1320
tttgacaaat gaccctataa ttctcagatc acctattata tattatgcta catatgaaaa 1380
aaactaacag atatcatgga taatctcaca aaagttcgtg agtatctcaa gtcctattct 1440
cgtctggatc aggcggtagg agagatagat gagatcgaag cacaacgagc tgaaaagtcc 1500
aattatgagt tgttccaaga ggatggagtg gaagagcata ctaagccctc ttattttcag 1560
gcagcagatg attctgacac agaatctgaa ccagaaattg aagacaatca aggtttgtat 1620
gcaccagatc cagaagctga gcaagttgaa ggctttatac aggggccttt agatgactat 1680
gcagatgagg aagtggatgt tgtatttact tcggactgga aacagcctga gcttgaatct 1740
gacgagcatg gaaagacctt acggttgaca tcgccagagg gtttaagtgg agagcagaaa 1800
tcccagtggc tttcgacgat taaagcagtc gtgcaaagtg ccaaatactg gaatctggca 1860
gagtgcacat ttgaagcatc gggagaaggg gtcattatga aggagcgcca gataactccg 1920
gatgtatata aggtcactcc agtgatgaac acacatccgt cccaatcaga agcagtatca 1980
gatgtttggt ctctctcaaa gacatccatg actttccaac ccaagaaagc aagtcttcag 2040
cctctcacca tatccttgga tgaattgttc tcatctagag gagagttcat ctctgtcgga 2100
ggtgacggac gaatgtctca taaagaggcc atcctgctcg gcctgagata caaaaagttg 2160
tacaatcagg cgagagtcaa atattctctg tagactatga aaaaaagtaa cagatatcac 2220
gatctaagtg ttatcccaat ccattcatca tgagttcctt aaagaagatt ctcggtctga 2280
aggggaaagg taagaaatct aagaaattag ggatcgcacc acccccttat gaagaggaca 2340
ctagcatgga gtatgctccg agcgctccaa ttgacaaatc ctattttgga gttgacgaga 2400
tggacaccta tgatccgaat caattaagat atgagaaatt cttctttaca gtgaaaatga 2460
cggttagatc taatcgtccg ttcagaacat actcagatgt ggcagccgct gtatcccatt 2520
gggatcacat gtacatcgga atggcaggga aacgtccctt ctacaaaatc ttggcttttt 2580
tgggttcttc taatctaaag gccactccag cggtattggc agatcaaggt caaccagagt 2640
atcacactca ctgcgaaggc agggcttatt tgccacatag gatggggaag acccctccca 2700
tgctcaatgt accagagcac ttcagaagac cattcaatat aggtctttac aagggaacga 2760
ttgagctcac aatgaccatc tacgatgatg agtcactgga agcagctcct atgatctggg 2820
atcatttcaa ttcttccaaa ttttctgatt tcagagagaa ggccttaatg tttggcctga 2880
ttgtcgagaa aaaggcatct ggagcgtggg tcctggattc tatcagccac ttcaaatgag 2940
ctagtctaac ttctagcttc tgaacaatcc ccggtttact cagtctctcc taattccagc 3000
ctctcgaaca actaatatcc tgtcttttct atccctatga aaaaaactaa cagagatcga 3060
tctgtttacg cgtgccacca tgttcgtgtt tctggtgctg ctgcctctgg tgagctccca 3120
gtgcgtgaac ttcaccacaa ggacccagct gccccctgcc tataccaatt ccttcacacg 3180
gggcgtgtac tatcccgaca aggtgttccg gagcagcgtg ctgcactcca cacaggatct 3240
gtttctgcct ttcttttcta acgtgacctg gttccacgcc atccacgtga gcggcaccaa 3300
tggcacaaag cggttcgcca atccagtgct gccctttaac gatggcgtgt acttcgcctc 3360
caccgagaag tctaacatca tcagaggctg gatctttggc accacactgg acagcaagac 3420
acagtccctg ctgatcgtga acaatgccac caacgtggtc atcaaggtgt gcgagttcca 3480
gttttgtaat gatccattcc tgggcgtgta ctatcacaag aacaataagt cttggatgga 3540
gagcgagttt cgcgtgtatt cctctgccaa caattgcaca tttgagtacg tgtcccagcc 3600
cttcctgatg gacctggagg gcaagcaggg caatttcaag aacctgaggg agttcgtgtt 3660
taagaatatc gatggctact tcaaaatcta ctccaagcac accccaatca acctggtgcg 3720
cggcctgcca cagggcttct ctgccctgga gccactggtg gatctgccca tcggcatcaa 3780
catcacccgg tttcagacac tgctggccct gcacagaagc tacctgacac caggcgacag 3840
ctcctctgga tggaccgcag gagcagcagc ctactatgtg ggctatctgc agcccaggac 3900
cttcctgctg aagtacaacg agaatggcac catcacagac gccgtggatt gcgccctgga 3960
tcccctgtct gagaccaagt gtacactgaa gagctttacc gtggagaagg gcatctatca 4020
gacaagcaat ttcagggtgc agcctaccga gtccatcgtg cgctttccca atatcacaaa 4080
cctgtgccct tttggcgagg tgttcaacgc aacccgcttc gccagcgtgt acgcctggaa 4140
taggaagcgc atctccaact gcgtggccga ctattctgtg ctgtacaaca gcgcctcctt 4200
ctctaccttt aagtgctatg gcgtgagccc cacaaagctg aatgacctgt gctttaccaa 4260
cgtgtacgcc gattccttcg tgatcagggg cgacgaggtg cgccagatcg caccaggaca 4320
gacaggcaat atcgcagact acaattataa gctgcctgac gatttcaccg gctgcgtgat 4380
cgcctggaac tctaacaatc tggatagcaa agtgggcggc aactacaatt atctgtaccg 4440
gctgtttaga aagtctaatc tgaagccatt cgagagggac atctccacag aaatctacca 4500
ggccggctct accccctgca atggcgtgaa gggctttaac tgttatttcc ctctgcagag 4560
ctacggcttc cagccaacat atggcgtggg ctatcagccc taccgcgtgg tggtgctgtc 4620
ttttgagctg ctgcacgcac ctgcaacagt gtgcggacca aagaagagca ccaatctggt 4680
gaagaacaag tgcgtgaact tcaacttcaa cggactgacc ggaacaggcg tgctgaccga 4740
gtccaacaag aagttcctgc cttttcagca gttcggcagg gacatcgcag ataccacaga 4800
cgccgtgcgc gaccctcaga ccctggagat cctggacatc acaccatgct ccttcggcgg 4860
cgtgtctgtg atcacaccag gcaccaatac aagcaaccag gtggccgtgc tgtatcaggg 4920
cgtgaattgt accgaggtgc cagtggcaat ccacgcagat cagctgaccc ctacatggcg 4980
ggtgtactct accggcagca acgtgttcca gacaagagcc ggatgcctga tcggagcaga 5040
gcacgtgaac aatagctatg agtgcgacat ccctatcggc gccggcatct gtgcctccta 5100
ccagacccag acaaactccc caaggtctgt gggcgataca ggcctgtcca agaatccaat 5160
cgagctggta gagggctggt tcagcagttg gaaaagctcc atcgcctcct ttttctttat 5220
catcggcctg atcatcggac tgttcctggt gctccgcgtg ggtatccacc tgtgcatcaa 5280
gctgaagcac accaagaaaa gacagattta tacagacatc gagatgaacc gcctgggaaa 5340
gggatccggc tccggcgagg gcaggggaag tctactaaca tgcggggacg tggaggaaaa 5400
tcccggcccc atgaagtgcc ttttgtactt agccttttta ttcattgggg tgaattgcaa 5460
gttcaccata gtttttccac acaaccaaaa aggaaactgg aaaaatgttc cttctaatta 5520
ccattattgc ccgtcaagct cagatttaaa ttggcataat gacttaatag gcacagccat 5580
acaagtcaaa atgcccaaga gtcacaaggc tattcaagca gacggttgga tgtgtcatgc 5640
ttccaaatgg gtcactactt gtgatttccg ctggtatgga ccgaagtata taacacagtc 5700
catccgatcc ttcactccat ctgtagaaca atgcaaggaa agcattgaac aaacgaaaca 5760
aggaacttgg ctgaatccag gcttccctcc tcaaagttgt ggatatgcaa ctgtgacgga 5820
tgccgaagca gtgattgtcc aggtgactcc tcaccatgtg ctggttgatg aatacacagg 5880
agaatgggtt gattcacagt tcatcaacgg aaaatgcagc aattacatat gccccactgt 5940
ccataactct acaacctggc attctgacta taaggtcaaa gggctatgtg attctaacct 6000
catttccatg gacatcacct tcttctcaga ggacggagag ctatcatccc tgggaaagga 6060
gggcacaggg ttcagaagta actactttgc ttatgaaact ggaggcaagg cctgcaaaat 6120
gcaatactgc aagcattggg gagtcagact cccatcaggt gtctggttcg agatggctga 6180
taaggatctc tttgctgcag ccagattccc tgaatgccca gaagggtcaa gtatctctgc 6240
tccatctcag acctcagtgg atgtaagtct aattcaggac gttgagagga tcttggatta 6300
ttccctctgc caagaaacct ggagcaaaat cagagcgggt cttccaatct ctccagtgga 6360
tctcagctat cttgctccta aaaacccagg aaccggtcct gctttcacca taatcaatgg 6420
taccctaaaa tactttgaga ccagatacat cagagtcgat attgctgctc caatcctctc 6480
aagaatggtc ggaatgatca gtggaactac cacagaaagg gaactgtggg atgactgggc 6540
accatatgaa gacgtggaaa ttggacccaa tggagttctg aggaccagtt caggatataa 6600
gtttccttta tacatgattg gacatggtat gttggactcc gatcttcatc ttagctcaaa 6660
ggctcaggtg ttcgaacatc ctcacattca agacgctgct tcgcaacttc ctgatgatga 6720
gagtttattt tttggtgata ctgggctatc caaaaatcca atcgagcttg tagaaggttg 6780
gttcagtagt tggaaaagct ctattgcctc ttttttcttt atcatagggt taatcattgg 6840
actattcttg gttctccgag ttggtatcca tctttgcatt aaattaaagc acaccaagaa 6900
aagacagatt tatacagaca tagagatgaa ccgacttgga aagtaagcta gccagattct 6960
tcatgtttgg accaaatcaa cttgtgatac catgctcaaa gaggcctcaa ttatatttga 7020
gtttttaatt tttatgaaaa aaactaacag caatcatgga agtccacgat tttgagaccg 7080
acgagttcaa tgatttcaat gaagatgact atgccacaag agaattcctg aatcccgatg 7140
agcgcatgac gtacttgaat catgctgatt acaatttgaa ttctcctcta attagtgatg 7200
atattgacaa tttgatcagg aaattcaatt ctcttccgat tccctcgatg tgggatagta 7260
agaactggga tggagttctt gagatgttaa catcatgtca agccaatccc atctcaacat 7320
ctcagatgca taaatggatg ggaagttggt taatgtctga taatcatgat gccagtcaag 7380
ggtatagttt tttacatgaa gtggacaaag aggcagaaat aacatttgac gtggtggaga 7440
ccttcatccg cggctggggc aacaaaccaa ttgaatacat caaaaaggaa agatggactg 7500
actcattcaa aattctcgct tatttgtgtc aaaagttttt ggacttacac aagttgacat 7560
taatcttaaa tgctgtctct gaggtggaat tgctcaactt ggcgaggact ttcaaaggca 7620
aagtcagaag aagttctcat ggaacgaaca tatgcaggat tagggttccc agcttgggtc 7680
ctacttttat ttcagaagga tgggcttact tcaagaaact tgatattcta atggaccgaa 7740
actttctgtt aatggtcaaa gatgtgatta tagggaggat gcaaacggtg ctatccatgg 7800
tatgtagaat agacaacctg ttctcagagc aagacatctt ctcccttcta aatatctaca 7860
gaattggaga taaaattgtg gagaggcagg gaaatttttc ttatgacttg attaaaatgg 7920
tggaaccgat atgcaacttg aagctgatga aattagcaag agaatcaagg cctttagtcc 7980
cacaattccc tcattttgaa aatcatatca agacttctgt tgatgaaggg gcaaaaattg 8040
accgaggtat aagattcctc catgatcaga taatgagtgt gaaaacagtg gatctcacac 8100
tggtgattta tggatcgttc agacattggg gtcatccttt tatagattat tacactggac 8160
tagaaaaatt acattcccaa gtaaccatga agaaagatat tgatgtgtca tatgcaaaag 8220
cacttgcaag tgatttagct cggattgttc tatttcaaca gttcaatgat cataaaaagt 8280
ggttcgtgaa tggagacttg ctccctcatg atcatccctt taaaagtcat gttaaagaaa 8340
atacatggcc cacagctgct caagttcaag attttggaga taaatggcat gaacttccgc 8400
tgattaaatg ttttgaaata cccgacttac tagacccatc gataatatac tctgacaaaa 8460
gtcattcaat gaataggtca gaggtgttga aacatgtccg aatgaatccg aacactccta 8520
tccctagtaa aaaggtgttg cagactatgt tggacacaaa ggctaccaat tggaaagaat 8580
ttcttaaaga gattgatgag aagggcttag atgatgatga tctaattatt ggtcttaaag 8640
gaaaggagag ggaactgaag ttggcaggta gatttttctc cctaatgtct tggaaattgc 8700
gagaatactt tgtaattacc gaatatttga taaagactca tttcgtccct atgtttaaag 8760
gcctgacaat ggcggacgat ctaactgcag tcattaaaaa gatgttagat tcctcatccg 8820
gccaaggatt gaagtcatat gaggcaattt gcatagccaa tcacattgat tacgaaaaat 8880
ggaataacca ccaaaggaag ttatcaaacg gcccagtgtt ccgagttatg ggccagttct 8940
taggttatcc atccttaatc gagagaactc atgaattttt tgagaaaagt cttatatact 9000
acaatggaag accagacttg atgcgtgttc acaacaacac actgatcaat tcaacctccc 9060
aacgagtttg ttggcaagga caagagggtg gactggaagg tctacggcaa aaaggatgga 9120
ctatcctcaa tctactggtt attcaaagag aggctaaaat cagaaacact gctgtcaaag 9180
tcttggcaca aggtgataat caagttattt gcacacagta taaaacgaag aaatcgagaa 9240
acgttgtaga attacagggt gctctcaatc aaatggtttc taataatgag aaaattatga 9300
ctgcaatcaa aatagggaca gggaagttag gacttttgat aaatgacgat gagactatgc 9360
aatctgcaga ttacttgaat tatggaaaaa taccgatttt ccgtggagtg attagagggt 9420
tagagaccaa gagatggtca cgagtgactt gtgtcaccaa tgaccaaata cccacttgtg 9480
ctaatataat gagctcagtt tccacaaatg ctctcaccgt agctcatttt gctgagaacc 9540
caatcaatgc catgatacag tacaattatt ttgggacatt tgctagactc ttgttgatga 9600
tgcatgatcc tgctcttcgt caatcattgt atgaagttca agataagata ccgggcttgc 9660
acagttctac tttcaaatac gccatgttgt atttggaccc ttccattgga ggagtgtcgg 9720
gcatgtcttt gtccaggttt ttgattagag ccttcccaga tcccgtaaca gaaagtctct 9780
cattctggag attcatccat gtacatgctc gaagtgagca tctgaaggag atgagtgcag 9840
tatttggaaa ccccgagata gccaagtttc gaataactca catagacaag ctagtagaag 9900
atccaacctc tctgaacatc gctatgggaa tgagtccagc gaacttgtta aagactgagg 9960
ttaaaaaatg cttaatcgaa tcaagacaaa ccatcaggaa ccaggtgatt aaggatgcaa 10020
ccatatattt gtatcatgaa gaggatcggc tcagaagttt cttatggtca ataaatcctc 10080
tgttccctag atttttaagt gaattcaaat caggcacttt tttgggagtc gcagacgggc 10140
tcatcagtct atttcaaaat tctcgtacta ttcggaactc ctttaagaaa aagtatcata 10200
gggaattgga tgatttgatt gtgaggagtg aggtatcctc tttgacacat ttagggaaac 10260
ttcatttgag aaggggatca tgtaaaatgt ggacatgttc agctactcat gctgacacat 10320
taagatacaa atcctggggc cgtacagtta ttgggacaac tgtaccccat ccattagaaa 10380
tgttgggtcc acaacatcga aaagagactc cttgtgcacc atgtaacaca tcagggttca 10440
attatgtttc tgtgcattgt ccagacggga tccatgacgt ctttagttca cggggaccat 10500
tgcctgctta tctagggtct aaaacatctg aatctacatc tattttgcag ccttgggaaa 10560
gggaaagcaa agtcccactg attaaaagag ctacacgtct tagagatgct atctcttggt 10620
ttgttgaacc cgactctaaa ctagcaatga ctatactttc taacatccac tctttaacag 10680
gcgaagaatg gaccaaaagg cagcatgggt tcaaaagaac agggtctgcc cttcataggt 10740
tttcgacatc tcggatgagc catggtgggt tcgcatctca gagcactgca gcattgacca 10800
ggttgatggc aactacagac accatgaggg atctgggaga tcagaatttc gactttttat 10860
tccaagcaac gttgctctat gctcaaatta ccaccactgt tgcaagagac ggatggatca 10920
ccagttgtac agatcattat catattgcct gtaagtcctg tttgagaccc atagaagaga 10980
tcaccctgga ctcaagtatg gactacacgc ccccagatgt atcccatgtg ctgaagacat 11040
ggaggaatgg ggaaggttcg tggggacaag agataaaaca gatctatcct ttagaaggga 11100
attggaagaa tttagcacct gctgagcaat cctatcaagt cggcagatgt ataggttttc 11160
tatatggaga cttggcgtat agaaaatcta ctcatgccga ggacagttct ctatttcctc 11220
tatctataca aggtcgtatt agaggtcgag gtttcttaaa agggttgcta gacggattaa 11280
tgagagcaag ttgctgccaa gtaatacacc ggagaagtct ggctcatttg aagaggccgg 11340
ccaacgcagt gtacggaggt ttgatttact tgattgataa attgagtgta tcacctccat 11400
tcctttctct tactagatca ggacctatta gagacgaatt agaaacgatt ccccacaaga 11460
tcccaacctc ctatccgaca agcaaccgtg atatgggggt gattgtcaga aattacttca 11520
aataccaatg ccgtctaatt gaaaagggaa aatacagatc acattattca caattatggt 11580
tattctcaga tgtcttatcc atagacttca ttggaccatt ctctatttcc accaccctct 11640
tgcaaatcct atacaagcca tttttatctg ggaaagataa gaatgagttg agagagctgg 11700
caaatctttc ttcattgcta agatcaggag aggggtggga agacatacat gtgaaattct 11760
tcaccaagga catattattg tgtccagagg aaatcagaca tgcttgcaag ttcgggattg 11820
ctaaggataa taataaagac atgagctatc ccccttgggg aagggaatcc agagggacaa 11880
ttacaacaat ccctgtttat tatacgacca ccccttaccc aaagatgcta gagatgcctc 11940
caagaatcca aaatcccctg ctgtccggaa tcaggttggg ccaattacca actggcgctc 12000
attataaaat tcggagtata ttacatggaa tgggaatcca ttacagggac ttcttgagtt 12060
gtggagacgg ctccggaggg atgactgctg cattactacg agaaaatgtg catagcagag 12120
gaatattcaa tagtctgtta gaattatcag ggtcagtcat gcgaggcgcc tctcctgagc 12180
cccccagtgc cctagaaact ttaggaggag ataaatcgag atgtgtaaat ggtgaaacat 12240
gttgggaata tccatctgac ttatgtgacc caaggacttg ggactatttc ctccgactca 12300
aagcaggctt ggggcttcaa attgatttaa ttgtaatgga tatggaagtt cgggattctt 12360
ctactagcct gaaaattgag acgaatgtta gaaattatgt gcaccggatt ttggatgagc 12420
aaggagtttt aatctacaag acttatggaa catatatttg tgagagcgaa aagaatgcag 12480
taacaatcct tggtcccatg ttcaagacgg tcgacttagt tcaaacagaa tttagtagtt 12540
ctcaaacgtc tgaagtatat atggtatgta aaggtttgaa gaaattaatc gatgaaccca 12600
atcccgattg gtcttccatc aatgaatcct ggaaaaacct gtacgcattc cagtcatcag 12660
aacaggaatt tgccagagca aagaaggtta gtacatactt taccttgaca ggtattccct 12720
cccaattcat tcctgatcct tttgtaaaca ttgagactat gctacaaata ttcggagtac 12780
ccacgggtgt gtctcatgcg gctgccttaa aatcatctga tagacctgca gatttattga 12840
ccattagcct tttttatatg gcgattatat cgtattataa catcaatcat atcagagtag 12900
gaccgatacc tccgaacccc ccatcagatg gaattgcaca aaatgtgggg atcgctataa 12960
ctggtataag cttttggctg agtttgatgg agaaagacat tccactatat caacagtgtt 13020
tagcagttat ccagcaatca ttcccgatta ggtgggaggc tgtttcagta aaaggaggat 13080
acaagcagaa gtggagtact agaggtgatg ggctcccaaa agatacccga acttcagact 13140
ccttggcccc aatcgggaac tggatcagat ctctggaatt ggtccgaaac caagttcgtc 13200
taaatccatt caatgagatc ttgttcaatc agctatgtcg tacagtggat aatcatttga 13260
aatggtcaaa tttgcgaaga aacacaggaa tgattgaatg gatcaataga cgaatttcaa 13320
aagaagaccg gtctatactg atgttgaaga gtgacctaca cgaggaaaac tcttggagag 13380
attaaaaaat catgaggaga ctccaaactt taagtatgaa aaaaactttg atccttaaga 13440
ccctcttgtg gtttttattt tttatctggt tttgtggtct tcgt 13484
<210> 26
<211> 12156
<212> DNA
<213> 人工序列
<220>
<223> VSV载体: Convac V5 China
<400> 26
acgaagacaa acaaaccatt attatcatta aaaggctcag gagaaacttt aacagtaatc 60
aaaatgtctg ttacagtcaa gagaatcatt gacaacacag tcatagttcc aaaacttcct 120
gcaaatgagg atccagtgga atacccggca gattacttca gaaaatcaaa ggagattcct 180
ctttacatca atactacaaa aagtttgtca gatctaagag gatatgtcta ccaaggcctc 240
aaatccggaa atgtatcaat catacatgtc aacagctact tgtatggagc attaaaggac 300
atccggggta agttggataa agattggtca agtttcggaa taaacatcgg gaaagcaggg 360
gatacaatcg gaatatttga ccttgtatcc ttgaaagccc tggacggcgt acttccagat 420
ggagtatcgg atgcttccag aaccagcgca gatgacaaat ggttgccttt gtatctactt 480
ggcttataca gagtgggcag aacacaaatg cctgaataca gaaaaaagct catggatggg 540
ctgacaaatc aatgcaaaat gatcaatgaa cagtttgaac ctcttgtgcc agaaggtcgt 600
gacatttttg atgtgtgggg aaatgacagt aattacacaa aaattgtcgc tgcagtggac 660
atgttcttcc acatgttcaa aaaacatgaa tgtgcctcgt tcagatacgg aactattgtt 720
tccagattca aagattgtgc tgcattggca acatttggac acctctgcaa aataaccgga 780
atgtctacag aagatgtaac gacctggatc ttgaaccgag aagttgcaga tgaaatggtc 840
caaatgatgc ttccaggcca agaaattgac aaggccgatt catacatgcc ttatttgatc 900
gactttggat tgtcttctaa gtctccatat tcttccgtca aaaaccctgc cttccacttc 960
tgggggcaat tgacagctct tctgctcaga tccaccagag caaggaatgc ccgacagcct 1020
gatgacattg agtatacatc tcttactaca gcaggtttgt tgtacgctta tgcagtagga 1080
tcctctgccg acttggcaca acagttttgt gttggagata acaaatacac tccagatgat 1140
agtaccggag gattgacgac taatgcaccg ccacaaggca gagatgtggt cgaatggctc 1200
ggatggtttg aagatcaaaa cagaaaaccg actcctgata tgatgcagta tgcgaaaaga 1260
gcagtcatgt cactgcaagg cctaagagag aagacaattg gcaagtatgc taagtcagaa 1320
tttgacaaat gaccctataa ttctcagatc acctattata tattatgcta catatgaaaa 1380
aaactaacag atatcatgga taatctcaca aaagttcgtg agtatctcaa gtcctattct 1440
cgtctggatc aggcggtagg agagatagat gagatcgaag cacaacgagc tgaaaagtcc 1500
aattatgagt tgttccaaga ggatggagtg gaagagcata ctaagccctc ttattttcag 1560
gcagcagatg attctgacac agaatctgaa ccagaaattg aagacaatca aggtttgtat 1620
gcaccagatc cagaagctga gcaagttgaa ggctttatac aggggccttt agatgactat 1680
gcagatgagg aagtggatgt tgtatttact tcggactgga aacagcctga gcttgaatct 1740
gacgagcatg gaaagacctt acggttgaca tcgccagagg gtttaagtgg agagcagaaa 1800
tcccagtggc tttcgacgat taaagcagtc gtgcaaagtg ccaaatactg gaatctggca 1860
gagtgcacat ttgaagcatc gggagaaggg gtcattatga aggagcgcca gataactccg 1920
gatgtatata aggtcactcc agtgatgaac acacatccgt cccaatcaga agcagtatca 1980
gatgtttggt ctctctcaaa gacatccatg actttccaac ccaagaaagc aagtcttcag 2040
cctctcacca tatccttgga tgaattgttc tcatctagag gagagttcat ctctgtcgga 2100
ggtgacggac gaatgtctca taaagaggcc atcctgctcg gcctgagata caaaaagttg 2160
tacaatcagg cgagagtcaa atattctctg tagactatga aaaaaagtaa cagatatcac 2220
gatctaagtg ttatcccaat ccattcatca tgagttcctt aaagaagatt ctcggtctga 2280
aggggaaagg taagaaatct aagaaattag ggatcgcacc acccccttat gaagaggaca 2340
ctagcatgga gtatgctccg agcgctccaa ttgacaaatc ctattttgga gttgacgaga 2400
tggacaccta tgatccgaat caattaagat atgagaaatt cttctttaca gtgaaaatga 2460
cggttagatc taatcgtccg ttcagaacat actcagatgt ggcagccgct gtatcccatt 2520
gggatcacat gtacatcgga atggcaggga aacgtccctt ctacaaaatc ttggcttttt 2580
tgggttcttc taatctaaag gccactccag cggtattggc agatcaaggt caaccagagt 2640
atcacactca ctgcgaaggc agggcttatt tgccacatag gatggggaag acccctccca 2700
tgctcaatgt accagagcac ttcagaagac cattcaatat aggtctttac aagggaacga 2760
ttgagctcac aatgaccatc tacgatgatg agtcactgga agcagctcct atgatctggg 2820
atcatttcaa ttcttccaaa ttttctgatt tcagagagaa ggccttaatg tttggcctga 2880
ttgtcgagaa aaaggcatct ggagcgtggg tcctggattc tatcagccac ttcaaatgag 2940
ctagtctaac ttctagcttc tgaacaatcc ccggtttact cagtctctcc taattccagc 3000
ctctcgaaca actaatatcc tgtcttttct atccctatga aaaaaactaa cagagatcga 3060
tctgtttacg cgtcactatg aagtgccttt tgtacttagc ctttttattc attggggtga 3120
attgcaagtt caccatagtt tttccacaca accaaaaagg aaactggaaa aatgttcctt 3180
ctaattacca ttattgcccg tcaagctcag atttaaattg gcataatgac ttaataggca 3240
cagccataca agtcaaaatg cccaagagtc acaaggctat tcaagcagac ggttggatgt 3300
gtcatgcttc caaatgggtc actacttgtg atttccgctg gtatggaccg aagtatataa 3360
cacagtccat ccgatccttc actccatctg tagaacaatg caaggaaagc attgaacaaa 3420
cgaaacaagg aacttggctg aatccaggct tccctcctca aagttgtgga tatgcaactg 3480
tgacggatgc cgaagcagtg attgtccagg tgactcctca ccatgtgctg gttgatgaat 3540
acacaggaga atgggttgat tcacagttca tcaacggaaa atgcagcaat tacatatgcc 3600
ccactgtcca taactctaca acctggcatt ctgactataa ggtcaaaggg ctatgtgatt 3660
ctaacctcat ttccatggac atcaccttct tctcagagga cggagagcta tcatccctgg 3720
gaaaggaggg cacagggttc agaagtaact actttgctta tgaaactgga ggcaaggcct 3780
gcaaaatgca atactgcaag cattggggag tcagactccc atcaggtgtc tggttcgaga 3840
tggctgataa ggatctcttt gctgcagcca gattccctga atgcccagaa gggtcaagta 3900
tctctgctcc atctcagacc tcagtggatg taagtctaat tcaggacgtt gagaggatct 3960
tggattattc cctctgccaa gaaacctgga gcaaaatcag agcgggtctt ccaatctctc 4020
cagtggatct cagctatctt gctcctaaaa acccaggaac cggtcctgct ttcaccataa 4080
tcaatggtac cctaaaatac tttgagacca gatacatcag agtcgatatt gctgctccaa 4140
tcctctcaag aatggtcgga atgatcagtg gaactaccac agaaagggaa ctgtgggatg 4200
actgggcacc atatgaagac gtggaaattg gacccaatgg agttctgagg accagttcag 4260
gatataagtt tcctttatac atgattggac atggtatgtt ggactccgat cttcatctta 4320
gctcaaaggc tcaggtgttc gaacatcctc acattcaaga cgctgcttcg caacttcctg 4380
atgatgagag tttatttttt ggtgatactg ggctatccaa aaatccaatc gagcttgtag 4440
aaggttggtt cagtagttgg aaaagctcta ttgcctcttt tttctttatc atagggttaa 4500
tcattggact attcttggtt ctccgagttg gtatccatct ttgcattaaa ttaaagcaca 4560
ccaagaaaag acagatttat acagacatag agatgaaccg acttggaaag taactcaaat 4620
cctgctaggt atgaaaaaaa ctaacagata tcacgctcga ggccaccatg aagtgcctgt 4680
tgtacttagc cttcctgttc atcggggtga attgccgctt tcccaatatc acaaacctgt 4740
gcccttttgg cgaggtgttc aacgcaaccc gcttcgccag cgtgtacgcc tggaatagga 4800
agcgcatctc caactgcgtg gccgactatt ctgtgctgta caacagcgcc tccttctcta 4860
cctttaagtg ctatggcgtg agccccacaa agctgaatga cctgtgcttt accaacgtgt 4920
acgccgattc cttcgtgatc aggggcgacg aggtgcgcca gatcgcacca ggacagacag 4980
gcaagatcgc agactacaat tataagctgc ctgacgattt caccggctgc gtgatcgcct 5040
ggaactctaa caatctggat agcaaagtgg gcggcaacta caattatctg taccggctgt 5100
ttagaaagtc taatctgaag ccattcgaga gggacatctc cacagaaatc taccaggccg 5160
gctctacccc ctgcaatggc gtggagggct ttaactgtta tttccctctg cagagctacg 5220
gcttccagcc aacaaacggc gtgggctatc agccctaccg cgtggtggtg ctgtcttttg 5280
agctgctgca cgcacctgca acagtgtgcg gaccaaagaa gagcaccaat ctggtgaaga 5340
acaagtgcgt gaacttcaac ggctctggat ccggctacat ccccgaggcc cccagagacg 5400
gccaggccta cgtgcggaag gacggcgagt gggtactgct cagcaccttc ctgggcagca 5460
gttggaaaag ctccatcgcc tcctttttct ttatcatcgg cctgatcatc ggactgttcc 5520
tggtgctccg cgtgggtatc cacctgtgca tcaagctgaa gcacaccaag aaaagacaga 5580
tttatacaga catcgagatg aaccgacttg gaaagtaagc tagccagatt cttcatgttt 5640
ggaccaaatc aacttgtgat accatgctca aagaggcctc aattatattt gagtttttaa 5700
tttttatgaa aaaaactaac agcaatcatg gaagtccacg attttgagac cgacgagttc 5760
aatgatttca atgaagatga ctatgccaca agagaattcc tgaatcccga tgagcgcatg 5820
acgtacttga atcatgctga ttacaatttg aattctcctc taattagtga tgatattgac 5880
aatttgatca ggaaattcaa ttctcttccg attccctcga tgtgggatag taagaactgg 5940
gatggagttc ttgagatgtt aacatcatgt caagccaatc ccatctcaac atctcagatg 6000
cataaatgga tgggaagttg gttaatgtct gataatcatg atgccagtca agggtatagt 6060
tttttacatg aagtggacaa agaggcagaa ataacatttg acgtggtgga gaccttcatc 6120
cgcggctggg gcaacaaacc aattgaatac atcaaaaagg aaagatggac tgactcattc 6180
aaaattctcg cttatttgtg tcaaaagttt ttggacttac acaagttgac attaatctta 6240
aatgctgtct ctgaggtgga attgctcaac ttggcgagga ctttcaaagg caaagtcaga 6300
agaagttctc atggaacgaa catatgcagg attagggttc ccagcttggg tcctactttt 6360
atttcagaag gatgggctta cttcaagaaa cttgatattc taatggaccg aaactttctg 6420
ttaatggtca aagatgtgat tatagggagg atgcaaacgg tgctatccat ggtatgtaga 6480
atagacaacc tgttctcaga gcaagacatc ttctcccttc taaatatcta cagaattgga 6540
gataaaattg tggagaggca gggaaatttt tcttatgact tgattaaaat ggtggaaccg 6600
atatgcaact tgaagctgat gaaattagca agagaatcaa ggcctttagt cccacaattc 6660
cctcattttg aaaatcatat caagacttct gttgatgaag gggcaaaaat tgaccgaggt 6720
ataagattcc tccatgatca gataatgagt gtgaaaacag tggatctcac actggtgatt 6780
tatggatcgt tcagacattg gggtcatcct tttatagatt attacactgg actagaaaaa 6840
ttacattccc aagtaaccat gaagaaagat attgatgtgt catatgcaaa agcacttgca 6900
agtgatttag ctcggattgt tctatttcaa cagttcaatg atcataaaaa gtggttcgtg 6960
aatggagact tgctccctca tgatcatccc tttaaaagtc atgttaaaga aaatacatgg 7020
cccacagctg ctcaagttca agattttgga gataaatggc atgaacttcc gctgattaaa 7080
tgttttgaaa tacccgactt actagaccca tcgataatat actctgacaa aagtcattca 7140
atgaataggt cagaggtgtt gaaacatgtc cgaatgaatc cgaacactcc tatccctagt 7200
aaaaaggtgt tgcagactat gttggacaca aaggctacca attggaaaga atttcttaaa 7260
gagattgatg agaagggctt agatgatgat gatctaatta ttggtcttaa aggaaaggag 7320
agggaactga agttggcagg tagatttttc tccctaatgt cttggaaatt gcgagaatac 7380
tttgtaatta ccgaatattt gataaagact catttcgtcc ctatgtttaa aggcctgaca 7440
atggcggacg atctaactgc agtcattaaa aagatgttag attcctcatc cggccaagga 7500
ttgaagtcat atgaggcaat ttgcatagcc aatcacattg attacgaaaa atggaataac 7560
caccaaagga agttatcaaa cggcccagtg ttccgagtta tgggccagtt cttaggttat 7620
ccatccttaa tcgagagaac tcatgaattt tttgagaaaa gtcttatata ctacaatgga 7680
agaccagact tgatgcgtgt tcacaacaac acactgatca attcaacctc ccaacgagtt 7740
tgttggcaag gacaagaggg tggactggaa ggtctacggc aaaaaggatg gactatcctc 7800
aatctactgg ttattcaaag agaggctaaa atcagaaaca ctgctgtcaa agtcttggca 7860
caaggtgata atcaagttat ttgcacacag tataaaacga agaaatcgag aaacgttgta 7920
gaattacagg gtgctctcaa tcaaatggtt tctaataatg agaaaattat gactgcaatc 7980
aaaataggga cagggaagtt aggacttttg ataaatgacg atgagactat gcaatctgca 8040
gattacttga attatggaaa aataccgatt ttccgtggag tgattagagg gttagagacc 8100
aagagatggt cacgagtgac ttgtgtcacc aatgaccaaa tacccacttg tgctaatata 8160
atgagctcag tttccacaaa tgctctcacc gtagctcatt ttgctgagaa cccaatcaat 8220
gccatgatac agtacaatta ttttgggaca tttgctagac tcttgttgat gatgcatgat 8280
cctgctcttc gtcaatcatt gtatgaagtt caagataaga taccgggctt gcacagttct 8340
actttcaaat acgccatgtt gtatttggac ccttccattg gaggagtgtc gggcatgtct 8400
ttgtccaggt ttttgattag agccttccca gatcccgtaa cagaaagtct ctcattctgg 8460
agattcatcc atgtacatgc tcgaagtgag catctgaagg agatgagtgc agtatttgga 8520
aaccccgaga tagccaagtt tcgaataact cacatagaca agctagtaga agatccaacc 8580
tctctgaaca tcgctatggg aatgagtcca gcgaacttgt taaagactga ggttaaaaaa 8640
tgcttaatcg aatcaagaca aaccatcagg aaccaggtga ttaaggatgc aaccatatat 8700
ttgtatcatg aagaggatcg gctcagaagt ttcttatggt caataaatcc tctgttccct 8760
agatttttaa gtgaattcaa atcaggcact tttttgggag tcgcagacgg gctcatcagt 8820
ctatttcaaa attctcgtac tattcggaac tcctttaaga aaaagtatca tagggaattg 8880
gatgatttga ttgtgaggag tgaggtatcc tctttgacac atttagggaa acttcatttg 8940
agaaggggat catgtaaaat gtggacatgt tcagctactc atgctgacac attaagatac 9000
aaatcctggg gccgtacagt tattgggaca actgtacccc atccattaga aatgttgggt 9060
ccacaacatc gaaaagagac tccttgtgca ccatgtaaca catcagggtt caattatgtt 9120
tctgtgcatt gtccagacgg gatccatgac gtctttagtt cacggggacc attgcctgct 9180
tatctagggt ctaaaacatc tgaatctaca tctattttgc agccttggga aagggaaagc 9240
aaagtcccac tgattaaaag agctacacgt cttagagatg ctatctcttg gtttgttgaa 9300
cccgactcta aactagcaat gactatactt tctaacatcc actctttaac aggcgaagaa 9360
tggaccaaaa ggcagcatgg gttcaaaaga acagggtctg cccttcatag gttttcgaca 9420
tctcggatga gccatggtgg gttcgcatct cagagcactg cagcattgac caggttgatg 9480
gcaactacag acaccatgag ggatctggga gatcagaatt tcgacttttt attccaagca 9540
acgttgctct atgctcaaat taccaccact gttgcaagag acggatggat caccagttgt 9600
acagatcatt atcatattgc ctgtaagtcc tgtttgagac ccatagaaga gatcaccctg 9660
gactcaagta tggactacac gcccccagat gtatcccatg tgctgaagac atggaggaat 9720
ggggaaggtt cgtggggaca agagataaaa cagatctatc ctttagaagg gaattggaag 9780
aatttagcac ctgctgagca atcctatcaa gtcggcagat gtataggttt tctatatgga 9840
gacttggcgt atagaaaatc tactcatgcc gaggacagtt ctctatttcc tctatctata 9900
caaggtcgta ttagaggtcg aggtttctta aaagggttgc tagacggatt aatgagagca 9960
agttgctgcc aagtaataca ccggagaagt ctggctcatt tgaagaggcc ggccaacgca 10020
gtgtacggag gtttgattta cttgattgat aaattgagtg tatcacctcc attcctttct 10080
cttactagat caggacctat tagagacgaa ttagaaacga ttccccacaa gatcccaacc 10140
tcctatccga caagcaaccg tgatatgggg gtgattgtca gaaattactt caaataccaa 10200
tgccgtctaa ttgaaaaggg aaaatacaga tcacattatt cacaattatg gttattctca 10260
gatgtcttat ccatagactt cattggacca ttctctattt ccaccaccct cttgcaaatc 10320
ctatacaagc catttttatc tgggaaagat aagaatgagt tgagagagct ggcaaatctt 10380
tcttcattgc taagatcagg agaggggtgg gaagacatac atgtgaaatt cttcaccaag 10440
gacatattat tgtgtccaga ggaaatcaga catgcttgca agttcgggat tgctaaggat 10500
aataataaag acatgagcta tcccccttgg ggaagggaat ccagagggac aattacaaca 10560
atccctgttt attatacgac caccccttac ccaaagatgc tagagatgcc tccaagaatc 10620
caaaatcccc tgctgtccgg aatcaggttg ggccaattac caactggcgc tcattataaa 10680
attcggagta tattacatgg aatgggaatc cattacaggg acttcttgag ttgtggagac 10740
ggctccggag ggatgactgc tgcattacta cgagaaaatg tgcatagcag aggaatattc 10800
aatagtctgt tagaattatc agggtcagtc atgcgaggcg cctctcctga gccccccagt 10860
gccctagaaa ctttaggagg agataaatcg agatgtgtaa atggtgaaac atgttgggaa 10920
tatccatctg acttatgtga cccaaggact tgggactatt tcctccgact caaagcaggc 10980
ttggggcttc aaattgattt aattgtaatg gatatggaag ttcgggattc ttctactagc 11040
ctgaaaattg agacgaatgt tagaaattat gtgcaccgga ttttggatga gcaaggagtt 11100
ttaatctaca agacttatgg aacatatatt tgtgagagcg aaaagaatgc agtaacaatc 11160
cttggtccca tgttcaagac ggtcgactta gttcaaacag aatttagtag ttctcaaacg 11220
tctgaagtat atatggtatg taaaggtttg aagaaattaa tcgatgaacc caatcccgat 11280
tggtcttcca tcaatgaatc ctggaaaaac ctgtacgcat tccagtcatc agaacaggaa 11340
tttgccagag caaagaaggt tagtacatac tttaccttga caggtattcc ctcccaattc 11400
attcctgatc cttttgtaaa cattgagact atgctacaaa tattcggagt acccacgggt 11460
gtgtctcatg cggctgcctt aaaatcatct gatagacctg cagatttatt gaccattagc 11520
cttttttata tggcgattat atcgtattat aacatcaatc atatcagagt aggaccgata 11580
cctccgaacc ccccatcaga tggaattgca caaaatgtgg ggatcgctat aactggtata 11640
agcttttggc tgagtttgat ggagaaagac attccactat atcaacagtg tttagcagtt 11700
atccagcaat cattcccgat taggtgggag gctgtttcag taaaaggagg atacaagcag 11760
aagtggagta ctagaggtga tgggctccca aaagataccc gaacttcaga ctccttggcc 11820
ccaatcggga actggatcag atctctggaa ttggtccgaa accaagttcg tctaaatcca 11880
ttcaatgaga tcttgttcaa tcagctatgt cgtacagtgg ataatcattt gaaatggtca 11940
aatttgcgaa gaaacacagg aatgattgaa tggatcaata gacgaatttc aaaagaagac 12000
cggtctatac tgatgttgaa gagtgaccta cacgaggaaa actcttggag agattaaaaa 12060
atcatgagga gactccaaac tttaagtatg aaaaaaactt tgatccttaa gaccctcttg 12120
tggtttttat tttttatctg gttttgtggt cttcgt 12156
<210> 27
<211> 12156
<212> DNA
<213> 人工序列
<220>
<223> VSV载体: Convac V5 South Africa
<400> 27
acgaagacaa acaaaccatt attatcatta aaaggctcag gagaaacttt aacagtaatc 60
aaaatgtctg ttacagtcaa gagaatcatt gacaacacag tcatagttcc aaaacttcct 120
gcaaatgagg atccagtgga atacccggca gattacttca gaaaatcaaa ggagattcct 180
ctttacatca atactacaaa aagtttgtca gatctaagag gatatgtcta ccaaggcctc 240
aaatccggaa atgtatcaat catacatgtc aacagctact tgtatggagc attaaaggac 300
atccggggta agttggataa agattggtca agtttcggaa taaacatcgg gaaagcaggg 360
gatacaatcg gaatatttga ccttgtatcc ttgaaagccc tggacggcgt acttccagat 420
ggagtatcgg atgcttccag aaccagcgca gatgacaaat ggttgccttt gtatctactt 480
ggcttataca gagtgggcag aacacaaatg cctgaataca gaaaaaagct catggatggg 540
ctgacaaatc aatgcaaaat gatcaatgaa cagtttgaac ctcttgtgcc agaaggtcgt 600
gacatttttg atgtgtgggg aaatgacagt aattacacaa aaattgtcgc tgcagtggac 660
atgttcttcc acatgttcaa aaaacatgaa tgtgcctcgt tcagatacgg aactattgtt 720
tccagattca aagattgtgc tgcattggca acatttggac acctctgcaa aataaccgga 780
atgtctacag aagatgtaac gacctggatc ttgaaccgag aagttgcaga tgaaatggtc 840
caaatgatgc ttccaggcca agaaattgac aaggccgatt catacatgcc ttatttgatc 900
gactttggat tgtcttctaa gtctccatat tcttccgtca aaaaccctgc cttccacttc 960
tgggggcaat tgacagctct tctgctcaga tccaccagag caaggaatgc ccgacagcct 1020
gatgacattg agtatacatc tcttactaca gcaggtttgt tgtacgctta tgcagtagga 1080
tcctctgccg acttggcaca acagttttgt gttggagata acaaatacac tccagatgat 1140
agtaccggag gattgacgac taatgcaccg ccacaaggca gagatgtggt cgaatggctc 1200
ggatggtttg aagatcaaaa cagaaaaccg actcctgata tgatgcagta tgcgaaaaga 1260
gcagtcatgt cactgcaagg cctaagagag aagacaattg gcaagtatgc taagtcagaa 1320
tttgacaaat gaccctataa ttctcagatc acctattata tattatgcta catatgaaaa 1380
aaactaacag atatcatgga taatctcaca aaagttcgtg agtatctcaa gtcctattct 1440
cgtctggatc aggcggtagg agagatagat gagatcgaag cacaacgagc tgaaaagtcc 1500
aattatgagt tgttccaaga ggatggagtg gaagagcata ctaagccctc ttattttcag 1560
gcagcagatg attctgacac agaatctgaa ccagaaattg aagacaatca aggtttgtat 1620
gcaccagatc cagaagctga gcaagttgaa ggctttatac aggggccttt agatgactat 1680
gcagatgagg aagtggatgt tgtatttact tcggactgga aacagcctga gcttgaatct 1740
gacgagcatg gaaagacctt acggttgaca tcgccagagg gtttaagtgg agagcagaaa 1800
tcccagtggc tttcgacgat taaagcagtc gtgcaaagtg ccaaatactg gaatctggca 1860
gagtgcacat ttgaagcatc gggagaaggg gtcattatga aggagcgcca gataactccg 1920
gatgtatata aggtcactcc agtgatgaac acacatccgt cccaatcaga agcagtatca 1980
gatgtttggt ctctctcaaa gacatccatg actttccaac ccaagaaagc aagtcttcag 2040
cctctcacca tatccttgga tgaattgttc tcatctagag gagagttcat ctctgtcgga 2100
ggtgacggac gaatgtctca taaagaggcc atcctgctcg gcctgagata caaaaagttg 2160
tacaatcagg cgagagtcaa atattctctg tagactatga aaaaaagtaa cagatatcac 2220
gatctaagtg ttatcccaat ccattcatca tgagttcctt aaagaagatt ctcggtctga 2280
aggggaaagg taagaaatct aagaaattag ggatcgcacc acccccttat gaagaggaca 2340
ctagcatgga gtatgctccg agcgctccaa ttgacaaatc ctattttgga gttgacgaga 2400
tggacaccta tgatccgaat caattaagat atgagaaatt cttctttaca gtgaaaatga 2460
cggttagatc taatcgtccg ttcagaacat actcagatgt ggcagccgct gtatcccatt 2520
gggatcacat gtacatcgga atggcaggga aacgtccctt ctacaaaatc ttggcttttt 2580
tgggttcttc taatctaaag gccactccag cggtattggc agatcaaggt caaccagagt 2640
atcacactca ctgcgaaggc agggcttatt tgccacatag gatggggaag acccctccca 2700
tgctcaatgt accagagcac ttcagaagac cattcaatat aggtctttac aagggaacga 2760
ttgagctcac aatgaccatc tacgatgatg agtcactgga agcagctcct atgatctggg 2820
atcatttcaa ttcttccaaa ttttctgatt tcagagagaa ggccttaatg tttggcctga 2880
ttgtcgagaa aaaggcatct ggagcgtggg tcctggattc tatcagccac ttcaaatgag 2940
ctagtctaac ttctagcttc tgaacaatcc ccggtttact cagtctctcc taattccagc 3000
ctctcgaaca actaatatcc tgtcttttct atccctatga aaaaaactaa cagagatcga 3060
tctgtttacg cgtcactatg aagtgccttt tgtacttagc ctttttattc attggggtga 3120
attgcaagtt caccatagtt tttccacaca accaaaaagg aaactggaaa aatgttcctt 3180
ctaattacca ttattgcccg tcaagctcag atttaaattg gcataatgac ttaataggca 3240
cagccataca agtcaaaatg cccaagagtc acaaggctat tcaagcagac ggttggatgt 3300
gtcatgcttc caaatgggtc actacttgtg atttccgctg gtatggaccg aagtatataa 3360
cacagtccat ccgatccttc actccatctg tagaacaatg caaggaaagc attgaacaaa 3420
cgaaacaagg aacttggctg aatccaggct tccctcctca aagttgtgga tatgcaactg 3480
tgacggatgc cgaagcagtg attgtccagg tgactcctca ccatgtgctg gttgatgaat 3540
acacaggaga atgggttgat tcacagttca tcaacggaaa atgcagcaat tacatatgcc 3600
ccactgtcca taactctaca acctggcatt ctgactataa ggtcaaaggg ctatgtgatt 3660
ctaacctcat ttccatggac atcaccttct tctcagagga cggagagcta tcatccctgg 3720
gaaaggaggg cacagggttc agaagtaact actttgctta tgaaactgga ggcaaggcct 3780
gcaaaatgca atactgcaag cattggggag tcagactccc atcaggtgtc tggttcgaga 3840
tggctgataa ggatctcttt gctgcagcca gattccctga atgcccagaa gggtcaagta 3900
tctctgctcc atctcagacc tcagtggatg taagtctaat tcaggacgtt gagaggatct 3960
tggattattc cctctgccaa gaaacctgga gcaaaatcag agcgggtctt ccaatctctc 4020
cagtggatct cagctatctt gctcctaaaa acccaggaac cggtcctgct ttcaccataa 4080
tcaatggtac cctaaaatac tttgagacca gatacatcag agtcgatatt gctgctccaa 4140
tcctctcaag aatggtcgga atgatcagtg gaactaccac agaaagggaa ctgtgggatg 4200
actgggcacc atatgaagac gtggaaattg gacccaatgg agttctgagg accagttcag 4260
gatataagtt tcctttatac atgattggac atggtatgtt ggactccgat cttcatctta 4320
gctcaaaggc tcaggtgttc gaacatcctc acattcaaga cgctgcttcg caacttcctg 4380
atgatgagag tttatttttt ggtgatactg ggctatccaa aaatccaatc gagcttgtag 4440
aaggttggtt cagtagttgg aaaagctcta ttgcctcttt tttctttatc atagggttaa 4500
tcattggact attcttggtt ctccgagttg gtatccatct ttgcattaaa ttaaagcaca 4560
ccaagaaaag acagatttat acagacatag agatgaaccg acttggaaag taactcaaat 4620
cctgctaggt atgaaaaaaa ctaacagata tcacgctcga ggccaccatg aagtgcctgt 4680
tgtacttagc cttcctgttc atcggggtga attgccgctt tcccaatatc acaaacctgt 4740
gcccttttgg cgaggtgttc aacgcaaccc gcttcgccag cgtgtacgcc tggaatagga 4800
agcgcatctc caactgcgtg gccgactatt ctgtgctgta caacagcgcc tccttctcta 4860
cctttaagtg ctatggcgtg agccccacaa agctgaatga cctgtgcttt accaacgtgt 4920
acgccgattc cttcgtgatc aggggcgacg aggtgcgcca gatcgcacca ggacagacag 4980
gcaatatcgc agactacaat tataagctgc ctgacgattt caccggctgc gtgatcgcct 5040
ggaactctaa caatctggat agcaaagtgg gcggcaacta caattatctg taccggctgt 5100
ttagaaagtc taatctgaag ccattcgaga gggacatctc cacagaaatc taccaggccg 5160
gctctacccc ctgcaatggc gtgaagggct ttaactgtta tttccctctg cagagctacg 5220
gcttccagcc aacatatggc gtgggctatc agccctaccg cgtggtggtg ctgtcttttg 5280
agctgctgca cgcacctgca acagtgtgcg gaccaaagaa gagcaccaat ctggtgaaga 5340
acaagtgcgt gaacttcaac ggctctggat ccggctacat ccccgaggcc cccagagacg 5400
gccaggccta cgtgcggaag gacggcgagt gggtactgct cagcaccttc ctgggcagca 5460
gttggaaaag ctccatcgcc tcctttttct ttatcatcgg cctgatcatc ggactgttcc 5520
tggtgctccg cgtgggtatc cacctgtgca tcaagctgaa gcacaccaag aaaagacaga 5580
tttatacaga catcgagatg aaccgacttg gaaagtaagc tagccagatt cttcatgttt 5640
ggaccaaatc aacttgtgat accatgctca aagaggcctc aattatattt gagtttttaa 5700
tttttatgaa aaaaactaac agcaatcatg gaagtccacg attttgagac cgacgagttc 5760
aatgatttca atgaagatga ctatgccaca agagaattcc tgaatcccga tgagcgcatg 5820
acgtacttga atcatgctga ttacaatttg aattctcctc taattagtga tgatattgac 5880
aatttgatca ggaaattcaa ttctcttccg attccctcga tgtgggatag taagaactgg 5940
gatggagttc ttgagatgtt aacatcatgt caagccaatc ccatctcaac atctcagatg 6000
cataaatgga tgggaagttg gttaatgtct gataatcatg atgccagtca agggtatagt 6060
tttttacatg aagtggacaa agaggcagaa ataacatttg acgtggtgga gaccttcatc 6120
cgcggctggg gcaacaaacc aattgaatac atcaaaaagg aaagatggac tgactcattc 6180
aaaattctcg cttatttgtg tcaaaagttt ttggacttac acaagttgac attaatctta 6240
aatgctgtct ctgaggtgga attgctcaac ttggcgagga ctttcaaagg caaagtcaga 6300
agaagttctc atggaacgaa catatgcagg attagggttc ccagcttggg tcctactttt 6360
atttcagaag gatgggctta cttcaagaaa cttgatattc taatggaccg aaactttctg 6420
ttaatggtca aagatgtgat tatagggagg atgcaaacgg tgctatccat ggtatgtaga 6480
atagacaacc tgttctcaga gcaagacatc ttctcccttc taaatatcta cagaattgga 6540
gataaaattg tggagaggca gggaaatttt tcttatgact tgattaaaat ggtggaaccg 6600
atatgcaact tgaagctgat gaaattagca agagaatcaa ggcctttagt cccacaattc 6660
cctcattttg aaaatcatat caagacttct gttgatgaag gggcaaaaat tgaccgaggt 6720
ataagattcc tccatgatca gataatgagt gtgaaaacag tggatctcac actggtgatt 6780
tatggatcgt tcagacattg gggtcatcct tttatagatt attacactgg actagaaaaa 6840
ttacattccc aagtaaccat gaagaaagat attgatgtgt catatgcaaa agcacttgca 6900
agtgatttag ctcggattgt tctatttcaa cagttcaatg atcataaaaa gtggttcgtg 6960
aatggagact tgctccctca tgatcatccc tttaaaagtc atgttaaaga aaatacatgg 7020
cccacagctg ctcaagttca agattttgga gataaatggc atgaacttcc gctgattaaa 7080
tgttttgaaa tacccgactt actagaccca tcgataatat actctgacaa aagtcattca 7140
atgaataggt cagaggtgtt gaaacatgtc cgaatgaatc cgaacactcc tatccctagt 7200
aaaaaggtgt tgcagactat gttggacaca aaggctacca attggaaaga atttcttaaa 7260
gagattgatg agaagggctt agatgatgat gatctaatta ttggtcttaa aggaaaggag 7320
agggaactga agttggcagg tagatttttc tccctaatgt cttggaaatt gcgagaatac 7380
tttgtaatta ccgaatattt gataaagact catttcgtcc ctatgtttaa aggcctgaca 7440
atggcggacg atctaactgc agtcattaaa aagatgttag attcctcatc cggccaagga 7500
ttgaagtcat atgaggcaat ttgcatagcc aatcacattg attacgaaaa atggaataac 7560
caccaaagga agttatcaaa cggcccagtg ttccgagtta tgggccagtt cttaggttat 7620
ccatccttaa tcgagagaac tcatgaattt tttgagaaaa gtcttatata ctacaatgga 7680
agaccagact tgatgcgtgt tcacaacaac acactgatca attcaacctc ccaacgagtt 7740
tgttggcaag gacaagaggg tggactggaa ggtctacggc aaaaaggatg gactatcctc 7800
aatctactgg ttattcaaag agaggctaaa atcagaaaca ctgctgtcaa agtcttggca 7860
caaggtgata atcaagttat ttgcacacag tataaaacga agaaatcgag aaacgttgta 7920
gaattacagg gtgctctcaa tcaaatggtt tctaataatg agaaaattat gactgcaatc 7980
aaaataggga cagggaagtt aggacttttg ataaatgacg atgagactat gcaatctgca 8040
gattacttga attatggaaa aataccgatt ttccgtggag tgattagagg gttagagacc 8100
aagagatggt cacgagtgac ttgtgtcacc aatgaccaaa tacccacttg tgctaatata 8160
atgagctcag tttccacaaa tgctctcacc gtagctcatt ttgctgagaa cccaatcaat 8220
gccatgatac agtacaatta ttttgggaca tttgctagac tcttgttgat gatgcatgat 8280
cctgctcttc gtcaatcatt gtatgaagtt caagataaga taccgggctt gcacagttct 8340
actttcaaat acgccatgtt gtatttggac ccttccattg gaggagtgtc gggcatgtct 8400
ttgtccaggt ttttgattag agccttccca gatcccgtaa cagaaagtct ctcattctgg 8460
agattcatcc atgtacatgc tcgaagtgag catctgaagg agatgagtgc agtatttgga 8520
aaccccgaga tagccaagtt tcgaataact cacatagaca agctagtaga agatccaacc 8580
tctctgaaca tcgctatggg aatgagtcca gcgaacttgt taaagactga ggttaaaaaa 8640
tgcttaatcg aatcaagaca aaccatcagg aaccaggtga ttaaggatgc aaccatatat 8700
ttgtatcatg aagaggatcg gctcagaagt ttcttatggt caataaatcc tctgttccct 8760
agatttttaa gtgaattcaa atcaggcact tttttgggag tcgcagacgg gctcatcagt 8820
ctatttcaaa attctcgtac tattcggaac tcctttaaga aaaagtatca tagggaattg 8880
gatgatttga ttgtgaggag tgaggtatcc tctttgacac atttagggaa acttcatttg 8940
agaaggggat catgtaaaat gtggacatgt tcagctactc atgctgacac attaagatac 9000
aaatcctggg gccgtacagt tattgggaca actgtacccc atccattaga aatgttgggt 9060
ccacaacatc gaaaagagac tccttgtgca ccatgtaaca catcagggtt caattatgtt 9120
tctgtgcatt gtccagacgg gatccatgac gtctttagtt cacggggacc attgcctgct 9180
tatctagggt ctaaaacatc tgaatctaca tctattttgc agccttggga aagggaaagc 9240
aaagtcccac tgattaaaag agctacacgt cttagagatg ctatctcttg gtttgttgaa 9300
cccgactcta aactagcaat gactatactt tctaacatcc actctttaac aggcgaagaa 9360
tggaccaaaa ggcagcatgg gttcaaaaga acagggtctg cccttcatag gttttcgaca 9420
tctcggatga gccatggtgg gttcgcatct cagagcactg cagcattgac caggttgatg 9480
gcaactacag acaccatgag ggatctggga gatcagaatt tcgacttttt attccaagca 9540
acgttgctct atgctcaaat taccaccact gttgcaagag acggatggat caccagttgt 9600
acagatcatt atcatattgc ctgtaagtcc tgtttgagac ccatagaaga gatcaccctg 9660
gactcaagta tggactacac gcccccagat gtatcccatg tgctgaagac atggaggaat 9720
ggggaaggtt cgtggggaca agagataaaa cagatctatc ctttagaagg gaattggaag 9780
aatttagcac ctgctgagca atcctatcaa gtcggcagat gtataggttt tctatatgga 9840
gacttggcgt atagaaaatc tactcatgcc gaggacagtt ctctatttcc tctatctata 9900
caaggtcgta ttagaggtcg aggtttctta aaagggttgc tagacggatt aatgagagca 9960
agttgctgcc aagtaataca ccggagaagt ctggctcatt tgaagaggcc ggccaacgca 10020
gtgtacggag gtttgattta cttgattgat aaattgagtg tatcacctcc attcctttct 10080
cttactagat caggacctat tagagacgaa ttagaaacga ttccccacaa gatcccaacc 10140
tcctatccga caagcaaccg tgatatgggg gtgattgtca gaaattactt caaataccaa 10200
tgccgtctaa ttgaaaaggg aaaatacaga tcacattatt cacaattatg gttattctca 10260
gatgtcttat ccatagactt cattggacca ttctctattt ccaccaccct cttgcaaatc 10320
ctatacaagc catttttatc tgggaaagat aagaatgagt tgagagagct ggcaaatctt 10380
tcttcattgc taagatcagg agaggggtgg gaagacatac atgtgaaatt cttcaccaag 10440
gacatattat tgtgtccaga ggaaatcaga catgcttgca agttcgggat tgctaaggat 10500
aataataaag acatgagcta tcccccttgg ggaagggaat ccagagggac aattacaaca 10560
atccctgttt attatacgac caccccttac ccaaagatgc tagagatgcc tccaagaatc 10620
caaaatcccc tgctgtccgg aatcaggttg ggccaattac caactggcgc tcattataaa 10680
attcggagta tattacatgg aatgggaatc cattacaggg acttcttgag ttgtggagac 10740
ggctccggag ggatgactgc tgcattacta cgagaaaatg tgcatagcag aggaatattc 10800
aatagtctgt tagaattatc agggtcagtc atgcgaggcg cctctcctga gccccccagt 10860
gccctagaaa ctttaggagg agataaatcg agatgtgtaa atggtgaaac atgttgggaa 10920
tatccatctg acttatgtga cccaaggact tgggactatt tcctccgact caaagcaggc 10980
ttggggcttc aaattgattt aattgtaatg gatatggaag ttcgggattc ttctactagc 11040
ctgaaaattg agacgaatgt tagaaattat gtgcaccgga ttttggatga gcaaggagtt 11100
ttaatctaca agacttatgg aacatatatt tgtgagagcg aaaagaatgc agtaacaatc 11160
cttggtccca tgttcaagac ggtcgactta gttcaaacag aatttagtag ttctcaaacg 11220
tctgaagtat atatggtatg taaaggtttg aagaaattaa tcgatgaacc caatcccgat 11280
tggtcttcca tcaatgaatc ctggaaaaac ctgtacgcat tccagtcatc agaacaggaa 11340
tttgccagag caaagaaggt tagtacatac tttaccttga caggtattcc ctcccaattc 11400
attcctgatc cttttgtaaa cattgagact atgctacaaa tattcggagt acccacgggt 11460
gtgtctcatg cggctgcctt aaaatcatct gatagacctg cagatttatt gaccattagc 11520
cttttttata tggcgattat atcgtattat aacatcaatc atatcagagt aggaccgata 11580
cctccgaacc ccccatcaga tggaattgca caaaatgtgg ggatcgctat aactggtata 11640
agcttttggc tgagtttgat ggagaaagac attccactat atcaacagtg tttagcagtt 11700
atccagcaat cattcccgat taggtgggag gctgtttcag taaaaggagg atacaagcag 11760
aagtggagta ctagaggtga tgggctccca aaagataccc gaacttcaga ctccttggcc 11820
ccaatcggga actggatcag atctctggaa ttggtccgaa accaagttcg tctaaatcca 11880
ttcaatgaga tcttgttcaa tcagctatgt cgtacagtgg ataatcattt gaaatggtca 11940
aatttgcgaa gaaacacagg aatgattgaa tggatcaata gacgaatttc aaaagaagac 12000
cggtctatac tgatgttgaa gagtgaccta cacgaggaaa actcttggag agattaaaaa 12060
atcatgagga gactccaaac tttaagtatg aaaaaaactt tgatccttaa gaccctcttg 12120
tggtttttat tttttatctg gttttgtggt cttcgt 12156
<210> 28
<211> 682
<212> PRT
<213> 人工序列
<220>
<223> SARS-CoV-2刺突蛋白(S) (682 aa)
<400> 28
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg
675 680
<210> 29
<211> 1273
<212> PRT
<213> 人工序列
<220>
<223> SARS-CoV-2刺突蛋白(S) (1273 aa)
<400> 29
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn
1010 1015 1020
Leu Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys
1025 1030 1035
Arg Val Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro
1040 1045 1050
Gln Ser Ala Pro His Gly Val Val Phe Leu His Val Thr Tyr Val
1055 1060 1065
Pro Ala Gln Glu Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His
1070 1075 1080
Asp Gly Lys Ala His Phe Pro Arg Glu Gly Val Phe Val Ser Asn
1085 1090 1095
Gly Thr His Trp Phe Val Thr Gln Arg Asn Phe Tyr Glu Pro Gln
1100 1105 1110
Ile Ile Thr Thr Asp Asn Thr Phe Val Ser Gly Asn Cys Asp Val
1115 1120 1125
Val Ile Gly Ile Val Asn Asn Thr Val Tyr Asp Pro Leu Gln Pro
1130 1135 1140
Glu Leu Asp Ser Phe Lys Glu Glu Leu Asp Lys Tyr Phe Lys Asn
1145 1150 1155
His Thr Ser Pro Asp Val Asp Leu Gly Asp Ile Ser Gly Ile Asn
1160 1165 1170
Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp Arg Leu Asn Glu
1175 1180 1185
Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu Gln Glu Leu
1190 1195 1200
Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile Trp Leu
1205 1210 1215
Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile Met
1220 1225 1230
Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys
1235 1240 1245
Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro
1250 1255 1260
Val Leu Lys Gly Val Lys Leu His Tyr Thr
1265 1270
<210> 30
<211> 781
<212> PRT
<213> 人工序列
<220>
<223> WuS1-RABVG (781 aa)
<400> 30
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Ser Val Gly Asp Glu Ala
675 680 685
Glu Asp Phe Val Glu Val His Leu Pro Asp Val His Asn Gln Val Ser
690 695 700
Gly Val Asp Leu Gly Leu Pro Asn Trp Gly Lys Tyr Val Leu Leu Ser
705 710 715 720
Ala Gly Ala Leu Thr Ala Leu Met Leu Ile Ile Phe Leu Met Thr Cys
725 730 735
Cys Arg Arg Val Asn Arg Ser Glu Pro Thr Gln His Asn Leu Arg Gly
740 745 750
Thr Gly Arg Glu Val Ser Val Thr Pro Gln Ser Gly Lys Ile Ile Ser
755 760 765
Ser Trp Glu Ser His Lys Ser Gly Gly Glu Thr Arg Leu
770 775 780
<210> 31
<211> 682
<212> PRT
<213> 人工序列
<220>
<223> WuS1-RABVG (682 aa)
<400> 31
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg
675 680
<210> 32
<211> 525
<212> PRT
<213> 人工序列
<220>
<223> SARS-CoV-2 核衣壳蛋白 (N)
<400> 32
Met Ala Thr Leu Leu Arg Ser Leu Ala Leu Phe Lys Arg Asn Lys Asp
1 5 10 15
Lys Pro Pro Ile Thr Ser Gly Ser Gly Gly Ala Ile Arg Gly Ile Lys
20 25 30
His Ile Ile Ile Val Pro Ile Pro Gly Asp Ser Ser Ile Thr Thr Arg
35 40 45
Ser Arg Leu Leu Asp Arg Leu Val Arg Leu Ile Gly Asn Pro Asp Val
50 55 60
Ser Gly Pro Lys Leu Thr Gly Ala Leu Ile Gly Ile Leu Ser Leu Phe
65 70 75 80
Val Glu Ser Pro Gly Gln Leu Ile Gln Arg Ile Thr Asp Asp Pro Asp
85 90 95
Val Ser Ile Arg Leu Leu Glu Val Val Gln Ser Asp Gln Ser Gln Ser
100 105 110
Gly Leu Thr Phe Ala Ser Arg Gly Thr Asn Met Glu Asp Glu Ala Asp
115 120 125
Gln Tyr Phe Ser His Asp Asp Pro Ile Ser Ser Asp Gln Ser Arg Phe
130 135 140
Gly Trp Phe Gly Asn Lys Glu Ile Ser Asp Ile Glu Val Gln Asp Pro
145 150 155 160
Glu Gly Phe Asn Met Ile Leu Gly Thr Ile Leu Ala Gln Ile Trp Val
165 170 175
Leu Leu Ala Lys Ala Val Thr Ala Pro Asp Thr Ala Ala Asp Ser Glu
180 185 190
Leu Arg Arg Trp Ile Lys Tyr Thr Gln Gln Arg Arg Val Val Gly Glu
195 200 205
Phe Arg Leu Glu Arg Lys Trp Leu Asp Val Val Arg Asn Arg Ile Ala
210 215 220
Glu Asp Leu Ser Leu Arg Arg Phe Met Val Ala Leu Ile Leu Asp Ile
225 230 235 240
Lys Arg Thr Pro Gly Asn Lys Pro Arg Ile Ala Glu Met Ile Cys Asp
245 250 255
Ile Asp Thr Tyr Ile Val Glu Ala Gly Leu Ala Ser Phe Ile Leu Thr
260 265 270
Ile Lys Phe Gly Ile Glu Thr Met Tyr Pro Ala Leu Gly Leu His Glu
275 280 285
Phe Ala Gly Glu Leu Ser Thr Leu Glu Ser Leu Met Asn Leu Tyr Gln
290 295 300
Gln Met Gly Glu Thr Ala Pro Tyr Met Val Ile Leu Glu Asn Ser Ile
305 310 315 320
Gln Asn Lys Phe Ser Ala Gly Ser Tyr Pro Leu Leu Trp Ser Tyr Ala
325 330 335
Met Gly Val Gly Val Glu Leu Glu Asn Ser Met Gly Gly Leu Asn Phe
340 345 350
Gly Arg Ser Tyr Phe Asp Pro Ala Tyr Phe Arg Leu Gly Gln Glu Met
355 360 365
Val Arg Arg Ser Ala Gly Lys Val Ser Ser Thr Leu Ala Ser Glu Leu
370 375 380
Gly Ile Thr Ala Glu Asp Ala Arg Leu Val Ser Glu Ile Ala Met His
385 390 395 400
Thr Thr Glu Asp Lys Ile Ser Arg Ala Val Gly Pro Arg Gln Ala Gln
405 410 415
Val Ser Phe Leu His Gly Asp Gln Ser Glu Asn Glu Leu Pro Arg Leu
420 425 430
Gly Gly Lys Glu Asp Arg Arg Val Lys Gln Ser Arg Gly Glu Ala Arg
435 440 445
Glu Ser Tyr Arg Glu Thr Gly Pro Ser Arg Ala Ser Asp Ala Arg Ala
450 455 460
Ala His Leu Pro Thr Gly Thr Pro Leu Asp Ile Asp Thr Ala Thr Glu
465 470 475 480
Ser Ser Gln Asp Pro Gln Asp Ser Arg Arg Ser Ala Asp Ala Leu Leu
485 490 495
Arg Leu Gln Ala Met Ala Gly Ile Ser Glu Glu Gln Gly Ser Asp Thr
500 505 510
Asp Thr Pro Ile Val Tyr Asn Asp Arg Asn Leu Leu Asp
515 520 525
<210> 33
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 33
cttaatgcgc cgctacagg 19
<210> 34
<211> 18
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 34
atgtgctgcg attaatta 18
<210> 35
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 35
tgtgctgatt aagtgtaag 19
<210> 36
<211> 17
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 36
gttttcccag tcacgac 17
<210> 37
<211> 48
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 37
aaacgacggc cagtggaatt ccgttaatac gactcactat aggaaagg 48
<210> 38
<211> 34
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 38
ggttgcgcgc cgttgactca ctataggggt tagg 34
<210> 39
<211> 39
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 39
gagagcgcgc atcgaaatta atacgactca ctatagata 39
<210> 40
<211> 39
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 40
gagacgtacg cgtaatacga ctcactatag gggagaggg 39
<210> 41
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 41
taatacatag ggtaatggg 19
<210> 42
<211> 46
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 42
gtgtcgtctc gcgcgtgcgg ccgcgctagc cagcttgggt ctccct 46
<210> 43
<211> 45
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 43
gtgtcgtctc tgggtaagga tagtgtcgtc tctggggtaa ggata 45
<210> 44
<211> 53
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 44
gtgtggtctc tgaggatagt tcagtgtggt ctctggtcgg taaggatagt tca 53
<210> 45
<211> 37
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 45
gagaaggttt gagaacgcgt ctcggtacgc cgggttt 37
<210> 46
<211> 34
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 46
tgtcacggat atccatcctg ctcttgtcct gtcc 34
<210> 47
<211> 36
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 47
cagtccaccg gtgtcacgga tatccctaat cctgct 36
<210> 48
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 48
ggattaggga tatccgagat ggccacactt 30
<210> 49
<211> 36
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 49
aagtgtggcc atctcggata tccctaatcc tgctct 36
<210> 50
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 50
tggccacact tttaaggagc t 21
<210> 51
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 51
ccaccggatc ctgatgtaat 20
<210> 52
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 52
ctggccttac cttcgcatca 20
<210> 53
<211> 25
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 53
aggattagcc agttttatcc tgact 25
<210> 54
<211> 17
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 54
agaagccagg agctaca 17
<210> 55
<211> 36
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 55
gtagtgtgcg atcgcgtgcg agaggccaga acaaca 36
<210> 56
<211> 60
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 56
gtgtacgcgt tccgccagaa caacagtgta cgcgttcctg acggagaggc cagaacaaca 60
<210> 57
<211> 82
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 57
gtgtgcggcc gctatagcgt aagtttttta taacaatggt gtgcggccgc tatagcgatc 60
tcctaagttt tttataacaa tg 82
<210> 58
<211> 67
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 58
gtgtgcggcc gttataacaa tggtgtgcgg ccgctataac gcgtttccta agttttttat 60
aacaatg 67
<210> 59
<211> 83
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 59
gtgtgcggcc gctataacgt aagtttttta taacaatggt gtgcggccgc tataacgcgt 60
ttcctaagtt ttttataaca atg 83
<210> 60
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 60
gacaacccag gacaggagc 19
<210> 61
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 61
actctcaatg ttcctccgcc 20
<210> 62
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 62
gccattcctg gacttgggaa 20
<210> 63
<211> 31
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 63
gtgtgcggcc gcaggttgta ctaggtgggt c 31
<210> 64
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 64
agtgattgcc tcccaaggtc 20
<210> 65
<211> 18
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 65
tgagttcgtg agctttcg 18
<210> 66
<211> 22
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 66
tctctgtaga ccgtagtgcc ca 22
<210> 67
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 67
caacccccga caaccagag 19
<210> 68
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 68
cacccctaaa ggagacaccg 20
<210> 69
<211> 44
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 69
gtgtgaatct caagtgtgaa gacttcatgc atcatgggtc tcaa 44
<210> 70
<211> 16
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 70
gagcgagcaa actact 16
<210> 71
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 71
cccaagtatg ttgcaaccca 20
<210> 72
<211> 22
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 72
tcgagcacta gcatagtcta ca 22
<210> 73
<211> 31
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 73
gtgttctaga tcagagcgac cttacatagg a 31
<210> 74
<211> 36
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 74
gtgtcgtctc tatgtcacca caacgagacc ggtgcg 36
<210> 75
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 75
cttgatcggg ttgctagcca 20
<210> 76
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 76
ccagggaatg tatgggggaa 20
<210> 77
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 77
atgcttccaa caggcgtgta 20
<210> 78
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 78
gttgcctata aagggggtcc c 21
<210> 79
<211> 18
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 79
ggggtccaat tacaggca 18
<210> 80
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 80
gtgttccatc ttgtgttcta gactatattg gttccatctt 40
<210> 81
<211> 55
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 81
gcagagacgc gtcttttttt ataacaatgg cagagacgcg tcttttataa caatg 55
<210> 82
<211> 56
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 82
gctataacgc gtatcttttt tataacaatg gctataacgc gtattttata acaatg 56
<210> 83
<211> 49
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 83
tatcactctg tgtttttata acaatgtatc actctgtttt ataacaatg 49
<210> 84
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 84
gtatgctcga gtccctcacg 20
<210> 85
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 85
tctctcgtga ccttgttgct 20
<210> 86
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 86
acggctgctg aaaatgttag g 21
<210> 87
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 87
agttcaagcc tagttcgcct 20
<210> 88
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 88
aggcttgaga cctctgtcct 20
<210> 89
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 89
atgaaacaag ggcagcatgc 20
<210> 90
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 90
agaagaggac gagggactgg 20
<210> 91
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 91
cgggttatga tcgggtgat 19
<210> 92
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 92
ttgttgcgtg atcccgatga 20
<210> 93
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 93
tcaatgctct aagccaccca 20
<210> 94
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 94
tcggcagcaa caacatctca 20
<210> 95
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 95
ccctacctct agtgtggggt 20
<210> 96
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 96
acggacctaa gctgtgcaaa 20
<210> 97
<211> 52
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 97
ctcgcgatat cctgccctcg cgatcgccta attgcggaac cctaatcctg cc 52
<210> 98
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 98
gccctaggtg gttaggcatt a 21
<210> 99
<211> 41
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 99
ccttacccaa ctttgtttgg tggccggcat agtcccagcc t 41
<210> 100
<211> 18
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 100
tcagcaaaaa acccctca 18
<210> 101
<211> 36
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 101
ggttgcgcgc atccggatat agttcctcct ttggtt 36
<210> 102
<211> 34
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 102
gaccatgatt acgccagcgg ccgcatccgg atat 34
<210> 103
<211> 24
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 103
agcggataac aatttcacac agga 24
<210> 104
<211> 24
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 104
tattaccgcc tttgagtgag ctga 24
<210> 105
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 105
ctttttacgg ttcctggcct 20
<210> 106
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 106
acatttcccc gaaaagtgc 19
权利要求书(按照条约第19条的修改)
1.一种编码重组病毒的分离核酸，所述重组病毒包括SARS-CoV-2刺突蛋白(S)或其部分。
2.一种编码选自狂犬病病毒、水疱性口炎病毒(VSV)和麻疹病毒的病毒的分离核酸，其中所述病毒包括SARS-CoV-2刺突蛋白(S)或其部分。
3.根据权利要求1所述的分离核酸，其中所述病毒是狂犬病病毒、水疱性口炎病毒(VSV)或麻疹病毒。
4.根据权利要求1所述的分离核酸，其中所述病毒是弹状病毒。
5.根据权利要求1-4中任一项所述的分离核酸，其中所述SARS-CoV-2刺突蛋白(S)的部分是所述SARS-CoV-2刺突蛋白(S)的受体结合位点。
6.根据权利要求1-4中任一项所述的分离核酸，其中所述SARS-CoV-2刺突蛋白(S)的部分是S1结构域。
7.根据权利要求1-4中任一项所述的分离核酸，其中所述分离核酸包括编码所述病毒的基因组的至少一部分的序列。
8.根据权利要求1-4中任一项所述的分离核酸，其中所述核酸包括(a)编码麻疹病毒的基因组的至少一部分的序列和(b)编码SARS-CoV-2刺突蛋白(S)或其部分的序列。
9.根据权利要求8所述的分离核酸，其中编码SARS-CoV-2刺突蛋白(S)或其部分的序列被插入所述麻疹病毒的基因组的第2、3或6位。
10.根据权利要求1-4中任一项所述的分离核酸，其中所述核酸包括(a)编码狂犬病病毒的基因组的至少一部分的序列和(b)编码包括SARS-CoV-2刺突蛋白(S)或其部分的蛋白质的序列。
11.根据权利要求10所述的分离核酸，其中所述狂犬病病毒的基因组的至少一部分包括N基因和P基因，并且编码包括SARS-CoV-2刺突蛋白(S)或其部分的蛋白质的序列被插入所述N基因和P基因之间的位置。
12.根据权利要求1-4中任一项所述的分离核酸，其中所述核酸包括(a)编码水疱性口炎病毒(VSV)的基因组的至少一部分的序列，和(b)编码包括SARS-CoV-2刺突蛋白(S)或其部分的蛋白质的序列。
13.根据权利要求10所述的分离核酸，其中所述包括SARS-CoV-2刺突蛋白(S)或其部分的蛋白质是包括糖蛋白(G)或其部分和SARS-CoV-2刺突蛋白(S)或其部分的融合蛋白。
14.根据权利要求13所述的分离核酸，其中所述融合蛋白包括与SARS-CoV-2刺突蛋白(S)的S1结构域融合的糖蛋白(G)或其部分。
15.根据权利要求1-4中任一项所述的分离核酸，其中所述核酸包括SEQ ID NO：1、SEQID NO：2、SEQ ID NO：3、SEQ ID NO：4或SEQ ID NO：5中所示的序列。
16.一种编码重组融合蛋白的分离核酸，所述重组融合蛋白包括(a)病毒的糖蛋白(G)或其部分，和(b)SARS-CoV-2刺突蛋白(S)或其部分。
17.根据权利要求16所述的分离核酸，其中所述病毒是狂犬病病毒或水疱性口炎病毒(VSV)。
18.根据权利要求16或17所述的分离核酸，其中所述SARS-CoV-2刺突蛋白(S)的部分是所述SARS-CoV-2刺突蛋白(S)的受体结合位点。
19.根据权利要求16或17所述的分离核酸，其中所述SARS-CoV-2刺突蛋白(S)的部分是S1结构域。
20.根据权利要求16或17所述的分离核酸，其中所述融合蛋白包括与所述SARS-CoV-2刺突蛋白(S)的S1结构域融合的狂犬病病毒糖蛋白(G)或其部分。
21.根据权利要求16或17所述的分离核酸，其中所述融合蛋白包括与所述SARS-CoV-2刺突蛋白(S)的S1结构域融合的水疱性口炎病毒(VSV)糖蛋白(G)或其部分。
22.一种包括SARS-CoV-2刺突蛋白(S)或其部分的重组病毒。
23.一种选自狂犬病病毒、水疱性口炎病毒(VSV)和麻疹病毒的重组病毒，其中所述病毒包括SARS-CoV-2刺突蛋白(S)或其部分。
24.根据权利要求22或23所述的重组病毒，其中所述病毒是狂犬病病毒、水疱性口炎病毒(VSV)或麻疹病毒。
25.根据权利要求22所述的重组病毒，其中所述病毒是弹状病毒。
26.根据权利要求22或23所述的重组病毒，其中所述SARS-CoV-2刺突蛋白(S)的部分是所述SARS-CoV-2刺突蛋白(S)的受体结合位点。
27.根据权利要求22或23所述的重组病毒，其中所述SARS-CoV-2刺突蛋白(S)的部分是S1结构域。
28.根据权利要求22或23所述的重组病毒，其中所述病毒包括融合蛋白，所述融合蛋白包括糖蛋白(G)或其部分和SARS-CoV-2刺突蛋白(S)或其部分。
29.根据权利要求28所述的重组病毒，其中所述融合蛋白包括与SARS-CoV-2刺突蛋白(S)的S1结构域融合的糖蛋白(G)或其部分。
30.根据权利要求22或23所述的重组病毒，其中所述病毒由根据权利要求1-15中任一项所述的核酸编码。
31.一种重组融合蛋白，其包括(a)病毒的糖蛋白(G)或其部分，和(b)SARS-CoV-2刺突蛋白(S)或其部分。
32.根据权利要求31所述的重组融合蛋白，其中所述病毒是狂犬病病毒或水疱性口炎病毒(VSV)。
33.根据权利要求31或32所述的重组融合蛋白，其中所述SARS-CoV-2刺突蛋白(S)的部分是所述SARS-CoV-2刺突蛋白(S)的受体结合位点。
34.根据权利要求31或32所述的重组融合蛋白，其中所述SARS-CoV-2刺突蛋白(S)的部分是S1结构域。
35.根据权利要求31或32所述的重组融合蛋白，其中所述融合蛋白包括与所述SARS-CoV-2刺突蛋白(S)的S1结构域融合的狂犬病病毒糖蛋白(G)或其部分。
36.根据权利要求31或32所述的重组融合蛋白，其中所述融合蛋白包括与所述SARS-CoV-2刺突蛋白(S)的S1结构域融合的水疱性口炎病毒(VSV)糖蛋白(G)或其部分。
37.根据权利要求31或32所述的重组融合蛋白，其中所述融合蛋白由根据权利要求16-21中任一项所述的核酸编码。
38.一种包括根据权利要求1-4中任一项所述的核酸的载体。
39.一种疫苗，其包括根据权利要求22或23所述的重组病毒或根据权利要求31或32所述的重组融合蛋白，以及药学上可接受的载体。
40.根据权利要求39所述的疫苗，进一步包括佐剂。
41.根据权利要求39所述的疫苗，其中所述佐剂是2％角鲨烯中的MPLA3D(6-酰基)
Figure FDA0003917092370000041
42.根据权利要求39所述的疫苗，其中所述病毒是灭活的。
43.一种在有需要的受试者中产生针对SARS-CoV-2病毒的免疫反应的方法，所述方法包括向受试者施用有效量的根据权利要求22或23所述的重组病毒、根据权利要求31或32所述的重组融合蛋白或根据权利要求39所述的疫苗。
44.一种向受试者接种针对SARS-CoV-2病毒的疫苗的方法，所述方法包括向受试者施用有效量的根据权利要求22或23所述的重组病毒、根据权利要求31或32所述的重组融合蛋白或根据权利要求39所述的疫苗。
45.一种在受试者中提供针对SARS-CoV-2病毒的免疫力的方法，所述方法包括向受试者施用有效量的根据权利要求22或23所述的重组病毒、根据权利要求31或32所述的重组融合蛋白或根据权利要求39所述的疫苗。
46.一种在有需要的受试者中治疗和/或预防与SARS-CoV-2病毒相关的疾病或紊乱的方法，所述方法包括向所述受试者施用有效量的根据权利要求22或23所述的重组病毒、根据权利要求31或32所述的重组融合蛋白或根据权利要求39所述的疫苗。
47.根据权利要求43所述的方法，其中所述受试者是人。
48.根据权利要求43所述的方法，其中所述受试者是猫。
49.根据权利要求43所述的方法，其中所述受试者是狗。
50.根据权利要求43所述的方法，其中所述受试者是雪貂。
51.根据权利要求46所述的方法，其中所述疾病或紊乱是COVID-19。
52.一种编码重组狂犬病病毒的分离核酸，所述重组狂犬病病毒包括与狂犬病糖蛋白(G)或其部分融合的SARS-CoV2刺突蛋白的S1结构域。
53.根据权利要求52所述的分离核酸，其中所述核酸包括(a)编码狂犬病病毒的基因组的至少一部分的序列，所述部分包括N基因和P基因，和(b)编码与所述糖蛋白(G)融合的S1结构域的序列，其中编码与所述糖蛋白(G)融合的S1结构域的序列被插入所述N基因和所述P基因之间的位置。
54.根据权利要求52或53所述的分离核酸，其中所述糖蛋白(G)包括在第333位用谷氨酸取代精氨酸的突变。
55.根据权利要求54所述的分离核酸，其中所述SARS-CoV2刺突蛋白的S1结构域与所述糖蛋白(G)的一部分融合，所述部分包括胞外结构域、胞质结构域和跨膜结构域。
56.根据权利要求55所述的分离核酸，其中所述糖蛋白(G)的部分包括所述胞外结构域的31个氨基酸和全长胞质结构域。
57.根据权利要求54所述的分离核酸，其中所述S1结构域与所述糖蛋白(G)的C-末端融合。
58.根据权利要求54所述的分离核酸，其中编码与所述糖蛋白(G)融合的S1结构域的序列被密码子优化以在细胞中表达。
59.一种由根据权利要求54所述的核酸编码的重组病毒。
60.根据权利要求59所述的重组病毒，其中所述病毒是灭活的。
61.一种疫苗，其包括根据权利要求59所述的重组病毒，以及药学上可接受的载体。
62.根据权利要求61所述的疫苗，进一步包括佐剂。
63.根据权利要求62所述的疫苗，其中所述佐剂是在2％角鲨烯中的MPLA 3D(6-酰基)
Figure FDA0003917092370000051
64.一种在有需要的受试者中产生针对SARS-CoV-2病毒的免疫反应的方法，所述方法包括向所述受试者施用有效量的根据权利要求59所述的重组病毒或根据权利要求61-63中任一项所述的疫苗。
65.一种向受试者接种针对SARS-CoV-2病毒的疫苗的方法，所述方法包括向所述受试者施用有效量的根据权利要求59所述的重组病毒或根据权利要求61-63中任一项所述的疫苗。
66.一种在受试者中提供针对SARS-CoV-2病毒的免疫力的方法，所述方法包括向所述受试者施用有效量的根据权利要求59所述的重组病毒或根据权利要求61-63中任一项所述的疫苗。
67.一种在有需要的受试者中治疗和/或预防与SARS-CoV-2病毒相关的疾病或紊乱的方法，所述方法包括向所述受试者施用有效量的根据权利要求59所述的重组病毒或根据权利要求61-63中任一项所述的疫苗。
68.根据权利要求64所述的方法，其中所述受试者是人。
69.根据权利要求64所述的方法，其中所述受试者是猫。
70.根据权利要求64所述的方法，其中所述受试者是狗。
71.根据权利要求64所述的方法，其中所述受试者是雪貂。
72.根据权利要求67所述的方法，其中所述疾病或紊乱是COVID-19。
73.一种在有需要的受试者中产生针对狂犬病病毒和SARS-CoV-2病毒的免疫反应的方法，所述方法包括向所述受试者施用有效量的包括重组狂犬病病毒的疫苗，所述重组狂犬病病毒包括SARS-CoV-2刺突蛋白(S)或其部分。
74.一种向受试者接种针对狂犬病病毒和SARS-CoV-2病毒的疫苗的方法，所述方法包括向所述受试者施用有效量的包括重组狂犬病病毒的疫苗，所述重组狂犬病病毒包括SARS-CoV-2刺突蛋白(S)或其部分。
75.一种在受试者中提供针对狂犬病病毒和SARS-CoV-2病毒的免疫力的方法，所述方法包括向所述受试者施用有效量的包括重组狂犬病病毒的疫苗，所述重组狂犬病病毒包括SARS-CoV-2刺突蛋白(S)或其部分。
76.一种在有需要的受试者中治疗和/或预防与狂犬病病毒相关的疾病或紊乱和与SARS-CoV-2病毒相关的疾病或紊乱的方法，所述方法包括向所述受试者施用有效量的包括重组狂犬病病毒的疫苗，所述重组狂犬病病毒包括SARS-CoV-2刺突蛋白(S)或其部分。
77.根据权利要求73-76中任一项所述的方法，其中所述重组狂犬病病毒包括与狂犬病糖蛋白(G)或其部分融合的SARS-CoV-2刺突蛋白的S1结构域。
78.根据权利要求73-76中任一项所述的方法，其中所述重组狂犬病病毒是灭活的。
79.根据权利要求73-76中任一项所述的方法，其中所述疫苗包括佐剂。
80.根据权利要求73-76中任一项所述的方法，其中所述受试者是非人类。
81.根据权利要求73-76中任一项所述的方法，其中所述受试者是哺乳动物。
82.根据权利要求73-76中任一项所述的方法，其中所述受试者是家养宠物或牲畜。
83.根据权利要求73-76中任一项所述的方法，其中所述受试者是猫。
84.根据权利要求73-76中任一项所述的方法，其中所述受试者是狗。
85.根据权利要求73-76中任一项所述的方法，其中所述受试者是雪貂。

Claims (85)

1.一种编码重组病毒的分离核酸，所述重组病毒包括SARS-CoV-2刺突蛋白(S)或其部分。
2.一种编码选自狂犬病病毒、水疱性口炎病毒(VSV)和麻疹病毒的病毒的分离核酸，其中所述病毒包括SARS-CoV-2刺突蛋白(S)或其部分。
3.根据权利要求1所述的分离核酸，其中所述病毒是狂犬病病毒、水疱性口炎病毒(VSV)或麻疹病毒。
4.根据权利要求1所述的分离核酸，其中所述病毒是弹状病毒。
5.根据权利要求1-4中任一项所述的分离核酸，其中所述SARS-CoV-2刺突蛋白(S)的部分是所述SARS-CoV-2刺突蛋白(S)的受体结合位点。
6.根据权利要求1-5中任一项所述的分离核酸，其中所述SARS-CoV-2刺突蛋白(S)的部分是S1结构域。
7.根据权利要求1-6中任一项所述的分离核酸，其中所述分离核酸包括编码所述病毒的基因组的至少一部分的序列。
8.根据权利要求1-7中任一项所述的分离核酸，其中所述核酸包括(a)编码麻疹病毒的基因组的至少一部分的序列和(b)编码SARS-CoV-2刺突蛋白(S)或其部分的序列。
9.根据权利要求8所述的分离核酸，其中编码SARS-CoV-2刺突蛋白(S)或其部分的序列被插入所述麻疹病毒的基因组的第2、3或6位。
10.根据权利要求1-7中任一项所述的分离核酸，其中所述核酸包括(a)编码狂犬病病毒的基因组的至少一部分的序列和(b)编码包括SARS-CoV-2刺突蛋白(S)或其部分的蛋白质的序列。
11.根据权利要求10所述的分离核酸，其中所述狂犬病病毒的基因组的至少一部分包括N基因和P基因，并且编码包括SARS-CoV-2刺突蛋白(S)或其部分的蛋白质的序列被插入所述N基因和P基因之间的位置。
12.根据权利要求1-7中任一项所述的分离核酸，其中所述核酸包括(a)编码水疱性口炎病毒(VSV)的基因组的至少一部分的序列，和(b)编码包括SARS-CoV-2刺突蛋白(S)或其部分的蛋白质的序列。
13.根据权利要求10-12中任一项所述的分离核酸，其中所述包括SARS-CoV-2刺突蛋白(S)或其部分的蛋白质是包括糖蛋白(G)或其部分和SARS-CoV-2刺突蛋白(S)或其部分的融合蛋白。
14.根据权利要求13所述的分离核酸，其中所述融合蛋白包括与SARS-CoV-2刺突蛋白(S)的S1结构域融合的糖蛋白(G)或其部分。
15.根据权利要求1-14中任一项所述的分离核酸，其中所述核酸包括SEQ ID NO：1、SEQID NO：2、SEQ ID NO：3、SEQ ID NO：4或SEQ ID NO：5中所示的序列。
16.一种编码重组融合蛋白的分离核酸，所述重组融合蛋白包括(a)病毒的糖蛋白(G)或其部分，和(b)SARS-CoV-2刺突蛋白(S)或其部分。
17.根据权利要求16所述的分离核酸，其中所述病毒是狂犬病病毒或水疱性口炎病毒(VSV)。
18.根据权利要求16或17所述的分离核酸，其中所述SARS-CoV-2刺突蛋白(S)的部分是所述SARS-CoV-2刺突蛋白(S)的受体结合位点。
19.根据权利要求16-18中任一项所述的分离核酸，其中所述SARS-CoV-2刺突蛋白(S)的部分是S1结构域。
20.根据权利要求16-19中任一项所述的分离核酸，其中所述融合蛋白包括与所述SARS-CoV-2刺突蛋白(S)的S1结构域融合的狂犬病病毒糖蛋白(G)或其部分。
21.根据权利要求16-19中任一项所述的分离核酸，其中所述融合蛋白包括与所述SARS-CoV-2刺突蛋白(S)的S1结构域融合的水疱性口炎病毒(VSV)糖蛋白(G)或其部分。
22.一种包括SARS-CoV-2刺突蛋白(S)或其部分的重组病毒。
23.一种选自狂犬病病毒、水疱性口炎病毒(VSV)和麻疹病毒的重组病毒，其中所述病毒包括SARS-CoV-2刺突蛋白(S)或其部分。
24.根据权利要求22或23所述的重组病毒，其中所述病毒是狂犬病病毒、水疱性口炎病毒(VSV)或麻疹病毒。
25.根据权利要求22所述的重组病毒，其中所述病毒是弹状病毒。
26.根据权利要求22-25中任一项所述的重组病毒，其中所述SARS-CoV-2刺突蛋白(S)的部分是所述SARS-CoV-2刺突蛋白(S)的受体结合位点。
27.根据权利要求22-26中任一项所述的重组病毒，其中所述SARS-CoV-2刺突蛋白(S)的部分是S1结构域。
28.根据权利要求22-27中任一项所述的重组病毒，其中所述病毒包括融合蛋白，所述融合蛋白包括糖蛋白(G)或其部分和SARS-CoV-2刺突蛋白(S)或其部分。
29.根据权利要求28所述的重组病毒，其中所述融合蛋白包括与SARS-CoV-2刺突蛋白(S)的S1结构域融合的糖蛋白(G)或其部分。
30.根据权利要求22-29中任一项所述的重组病毒，其中所述病毒由根据权利要求1-15中任一项所述的核酸编码。
31.一种重组融合蛋白，其包括(a)病毒的糖蛋白(G)或其部分，和(b)SARS-CoV-2刺突蛋白(S)或其部分。
32.根据权利要求31所述的重组融合蛋白，其中所述病毒是狂犬病病毒或水疱性口炎病毒(VSV)。
33.根据权利要求31或32所述的重组融合蛋白，其中所述SARS-CoV-2刺突蛋白(S)的部分是所述SARS-CoV-2刺突蛋白(S)的受体结合位点。
34.根据权利要求31-33中任一项所述的重组融合蛋白，其中所述SARS-CoV-2刺突蛋白(S)的部分是S1结构域。
35.根据权利要求31-34中任一项所述的重组融合蛋白，其中所述融合蛋白包括与所述SARS-CoV-2刺突蛋白(S)的S1结构域融合的狂犬病病毒糖蛋白(G)或其部分。
36.根据权利要求31-34中任一项所述的重组融合蛋白，其中所述融合蛋白包括与所述SARS-CoV-2刺突蛋白(S)的S1结构域融合的水疱性口炎病毒(VSV)糖蛋白(G)或其部分。
37.根据权利要求31-36中任一项所述的重组融合蛋白，其中所述融合蛋白由根据权利要求16-21中任一项所述的核酸编码。
38.一种包括根据权利要求1-21中任一项所述的核酸的载体。
39.一种疫苗，其包括根据权利要求22-30中任一项所述的重组病毒或根据权利要求31-37中任一项所述的重组融合蛋白，以及药学上可接受的载体。
40.根据权利要求39所述的疫苗，进一步包括佐剂。
41.根据权利要求39或40所述的疫苗，其中所述佐剂是2％角鲨烯中的MPLA 3D(6-酰基)
Figure FDA0003917092290000041
42.根据权利要求39-41中任一项所述的疫苗，其中所述病毒是灭活的。
43.一种在有需要的受试者中产生针对SARS-CoV-2病毒的免疫反应的方法，所述方法包括向受试者施用有效量的根据权利要求22-30中任一项所述的重组病毒、根据权利要求31-37中任一项所述的重组融合蛋白或根据权利要求39-42中任一项所述的疫苗。
44.一种向受试者接种针对SARS-CoV-2病毒的疫苗的方法，所述方法包括向受试者施用有效量的根据权利要求22-30中任一项所述的重组病毒、根据权利要求31-37中任一项所述的重组融合蛋白或根据权利要求39-42中任一项所述的疫苗。
45.一种在受试者中提供针对SARS-CoV-2病毒的免疫力的方法，所述方法包括向受试者施用有效量的根据权利要求22-30中任一项所述的重组病毒、根据权利要求31-37中任一项所述的重组融合蛋白或根据权利要求39-42中任一项所述的疫苗。
46.一种在有需要的受试者中治疗和/或预防与SARS-CoV-2病毒相关的疾病或紊乱的方法，所述方法包括向所述受试者施用有效量的根据权利要求22-30中任一项所述的重组病毒、根据权利要求31-37中任一项所述的重组融合蛋白或根据权利要求39-42中任一项所述的疫苗。
47.根据权利要求42-46中任一项所述的方法，其中所述受试者是人。
48.根据权利要求42-46中任一项所述的方法，其中所述受试者是猫。
49.根据权利要求42-46中任一项所述的方法，其中所述受试者是狗。
50.根据权利要求42-46中任一项所述的方法，其中所述受试者是雪貂。
51.根据权利要求46所述的方法，其中所述疾病或紊乱是COVID-19。
52.一种编码重组狂犬病病毒的分离核酸，所述重组狂犬病病毒包括与狂犬病糖蛋白(G)或其部分融合的SARS-CoV2刺突蛋白的S1结构域。
53.根据权利要求52所述的分离核酸，其中所述核酸包括(a)编码狂犬病病毒的基因组的至少一部分的序列，所述部分包括N基因和P基因，和(b)编码与所述糖蛋白(G)融合的S1结构域的序列，其中编码与所述糖蛋白(G)融合的S1结构域的序列被插入所述N基因和所述P基因之间的位置。
54.根据权利要求52或53所述的分离核酸，其中所述糖蛋白(G)包括在第333位用谷氨酸取代精氨酸的突变。
55.根据权利要求52-54中任一项所述的分离核酸，其中所述SARS-CoV2刺突蛋白的S1结构域与所述糖蛋白(G)的一部分融合，所述部分包括胞外结构域、胞质结构域和跨膜结构域。
56.根据权利要求55所述的分离核酸，其中所述糖蛋白(G)的部分包括所述胞外结构域的31个氨基酸和全长胞质结构域。
57.根据权利要求52-56中任一项所述的分离核酸，其中所述S1结构域与所述糖蛋白(G)的C-末端融合。
58.根据权利要求53-57中任一项所述的分离核酸，其中编码与所述糖蛋白(G)融合的S1结构域的序列被密码子优化以在细胞中表达。
59.一种由根据权利要求52-58中任一项所述的核酸编码的重组病毒。
60.根据权利要求59所述的重组病毒，其中所述病毒是灭活的。
61.一种疫苗，其包括根据权利要求59或60所述的重组病毒，以及药学上可接受的载体。
62.根据权利要求61所述的疫苗，进一步包括佐剂。
63.根据权利要求62所述的疫苗，其中所述佐剂是在2％角鲨烯中的MPLA 3D(6-酰基)
Figure FDA0003917092290000051
64.一种在有需要的受试者中产生针对SARS-CoV-2病毒的免疫反应的方法，所述方法包括向所述受试者施用有效量的根据权利要求59-60中任一项所述的重组病毒或根据权利要求61-63中任一项所述的疫苗。
65.一种向受试者接种针对SARS-CoV-2病毒的疫苗的方法，所述方法包括向所述受试者施用有效量的根据权利要求59-60中任一项所述的重组病毒或根据权利要求61-63中任一项所述的疫苗。
66.一种在受试者中提供针对SARS-CoV-2病毒的免疫力的方法，所述方法包括向所述受试者施用有效量的根据权利要求59-60中任一项所述的重组病毒或根据权利要求61-63中任一项所述的疫苗。
67.一种在有需要的受试者中治疗和/或预防与SARS-CoV-2病毒相关的疾病或紊乱的方法，所述方法包括向所述受试者施用有效量的根据权利要求59-60中任一项所述的重组病毒或根据权利要求61-63中任一项所述的疫苗。
68.根据权利要求64-67中任一项所述的方法，其中所述受试者是人。
69.根据权利要求64-67中任一项所述的方法，其中所述受试者是猫。
70.根据权利要求64-67中任一项所述的方法，其中所述受试者是狗。
71.根据权利要求64-67中任一项所述的方法，其中所述受试者是雪貂。
72.根据权利要求67所述的方法，其中所述疾病或紊乱是COVID-19。
73.一种在有需要的受试者中产生针对狂犬病病毒和SARS-CoV-2病毒的免疫反应的方法，所述方法包括向所述受试者施用有效量的包括重组狂犬病病毒的疫苗，所述重组狂犬病病毒包括SARS-CoV-2刺突蛋白(S)或其部分。
74.一种向受试者接种针对狂犬病病毒和SARS-CoV-2病毒的疫苗的方法，所述方法包括向所述受试者施用有效量的包括重组狂犬病病毒的疫苗，所述重组狂犬病病毒包括SARS-CoV-2刺突蛋白(S)或其部分。
75.一种在受试者中提供针对狂犬病病毒和SARS-CoV-2病毒的免疫力的方法，所述方法包括向所述受试者施用有效量的包括重组狂犬病病毒的疫苗，所述重组狂犬病病毒包括SARS-CoV-2刺突蛋白(S)或其部分。
76.一种在有需要的受试者中治疗和/或预防与狂犬病病毒相关的疾病或紊乱和与SARS-CoV-2病毒相关的疾病或紊乱的方法，所述方法包括向所述受试者施用有效量的包括重组狂犬病病毒的疫苗，所述重组狂犬病病毒包括SARS-CoV-2刺突蛋白(S)或其部分。
77.根据权利要求73-76中任一项所述的方法，其中所述重组狂犬病病毒包括与狂犬病糖蛋白(G)或其部分融合的SARS-CoV-2刺突蛋白的S1结构域。
78.根据权利要求73-77中任一项所述的方法，其中所述重组狂犬病病毒是灭活的。
79.根据权利要求73-78中任一项所述的方法，其中所述疫苗包括佐剂。
80.根据权利要求73-79中任一项所述的方法，其中所述受试者是非人类。
81.根据权利要求73-80中任一项所述的方法，其中所述受试者是哺乳动物。
82.根据权利要求73-81中任一项所述的方法，其中所述受试者是家养宠物或牲畜。
83.根据权利要求73-82中任一项所述的方法，其中所述受试者是猫。
84.根据权利要求73-82中任一项所述的方法，其中所述受试者是狗。
85.根据权利要求73-82中任一项所述的方法，其中所述受试者是雪貂。
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