CN116172187A - Method for removing ganoderma lucidum spore powder wall, application and wall-removed ganoderma lucidum spore powder drink - Google Patents
Method for removing ganoderma lucidum spore powder wall, application and wall-removed ganoderma lucidum spore powder drink Download PDFInfo
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- CN116172187A CN116172187A CN202310219439.2A CN202310219439A CN116172187A CN 116172187 A CN116172187 A CN 116172187A CN 202310219439 A CN202310219439 A CN 202310219439A CN 116172187 A CN116172187 A CN 116172187A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
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- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
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- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The invention discloses a method for removing ganoderma lucidum spore powder wall, application and a wall-removed ganoderma lucidum spore powder drink, wherein wall-broken ganoderma lucidum spore powder is filled into a capsule cup for sealing and then is put into a capsule coffee machine; the extraction needle pierces the capsule cup, high-pressure water flow enters the capsule cup through the extraction needle to remove the wall, the water flow pushes through the capsule sealing membrane, and the water flow flows out through the filter screen to obtain the wall-removed ganoderma lucidum spore powder liquid. The method of the invention does not need special equipment, can quickly and efficiently remove the wall of the ganoderma lucidum spore powder only by using a common household capsule coffee machine, can directly drink the obtained ganoderma lucidum spore powder, improves the edible taste of the ganoderma lucidum spore powder, has better effect of improving immunity compared with the wall-removed ganoderma lucidum spore powder in the prior art, and has simple operation method and better wall-removed effect and stability.
Description
Technical Field
The invention relates to the field of removing the wall of ganoderma lucidum spore powder, in particular to a method for removing the wall of ganoderma lucidum spore powder, application and a wall-removed ganoderma lucidum spore powder drink.
Background
The Ganoderma spore powder is prepared by continuously ejecting spores from fungus tubes after Ganoderma fruiting body is mature. The Ganoderma spore contains almost all active substances including polysaccharides, triterpenes, proteins, sterols, nucleosides, microelements, etc., and part of active substances such as total triterpenes and total polysaccharides are obviously higher than Ganoderma fruiting body. In recent years, the ganoderma lucidum spore powder has the effects of resisting cancer and tumor, protecting nervous system, protecting liver, reducing blood fat, reducing blood sugar, regulating organism immunity and the like. In addition, the ganoderma lucidum spore powder medicine is widely applied to clinical aspects, and is safe and nontoxic.
The ganoderma lucidum spores have a double-wall structure, the double walls are composed of chitin and cellulose, the texture is hard, active ingredients are wrapped and are not easy to dissolve out, and absorption of human bodies is affected. The ganoderma lucidum spore wall accounts for 65% of the spores, the ganoderma lucidum spore oil accounts for 25%, and other effective components account for 10%. The ganoderma lucidum spore powder in the market is subjected to wall breaking treatment mainly by a physical method, a chemical method, a biological method and the like so as to increase the absorption of active ingredients of the ganoderma lucidum spore powder, but the ganoderma lucidum spore wall still remains in the ganoderma lucidum spore powder to influence the taste, and the absorption and utilization rate is low. Chinese patent CN104013652a discloses a refining process and comprehensive utilization method of ganoderma lucidum spore powder, and uses the wall-broken ganoderma lucidum spore powder as raw material, then makes deep processing, and removes part of ganoderma lucidum spore wall shell (65% of non-medicinal part) by 30 processes of extraction, separation, wall removing, purification, concentration, refining, etc., but the process is complex, time-consuming and greatly increases cost, and the organic solvent residue is easily caused by the process of extracting with organic solvent, which is unfavorable for health. Chinese patent CN114949009a discloses an environment-friendly preparation process of wall-removed ganoderma lucidum spore powder integrating wall breaking and extraction, and wall removal is only carried out by a filtration mode after wall breaking, and finally, sufficient wall removal cannot be realized.
Disclosure of Invention
The first object of the invention is to provide a method for removing ganoderma lucidum spore powder wall.
In order to achieve the technical purpose, the invention adopts the following technical scheme:
a method for removing ganoderma lucidum spore powder wall, comprising:
filling the wall-broken ganoderma lucidum spore powder into a capsule cup, sealing, and then placing into a capsule coffee machine;
the extraction needle pierces the capsule cup, high-pressure water flow enters the capsule cup through the extraction needle to remove the wall, the water flow pushes through the capsule sealing membrane and flows out through the filter screen to obtain wall-removed ganoderma lucidum spore powder liquid;
wherein the water flow pressure is more than or equal to 1bar, the water temperature is more than or equal to 20 ℃, and the wall removing time is less than 5min.
As a preferred embodiment, the water flow pressure is 1-30bar and the water temperature is 20-110 ℃.
As a preferred embodiment, the wall removal time is less than or equal to 1min.
As a preferred embodiment, after the wall-broken ganoderma lucidum spore powder is added into a capsule cup, an aluminum foil cover is used for sealing the mouth of the capsule cup, and the capsule cup is placed on a hot-melting sealing machine for hot-melting sealing.
The second object of the invention is to provide the application of the capsule coffee machine in removing the wall of ganoderma lucidum spore powder.
Specifically, adding wall-broken ganoderma lucidum spore powder into a capsule cup, then placing into a capsule coffee machine, removing the wall for less than 5 minutes under the conditions that the water temperature is more than or equal to 1bar and the water temperature is more than or equal to 20 ℃ to obtain wall-removed ganoderma lucidum spore powder liquid.
As a preferred embodiment, the wall is removed at 20-110℃and 1-30 bar.
As a preferred embodiment, the wall removal time is < 1min.
As a preferred embodiment, after the wall-broken ganoderma lucidum spore powder is added into a capsule cup, an aluminum foil cover is used for sealing the mouth of the capsule cup, and the capsule cup is placed on a hot-melting sealing machine for hot-melting sealing.
The third object of the invention is to provide a wall-removed ganoderma lucidum spore powder drink, which is prepared by adding wall-broken ganoderma lucidum spore powder into a capsule cup, then placing the capsule cup into a capsule coffee machine, and removing the wall for less than 5 minutes under the conditions that the water temperature is more than or equal to 1bar and the water temperature is more than or equal to 20 ℃.
By utilizing the method, the ganoderma lucidum spore powder wall can be removed rapidly and efficiently only by using a common household capsule coffee machine, the obtained ganoderma lucidum spore powder liquid can be directly drunk, the edible taste of the ganoderma lucidum spore powder is improved, and compared with the wall-removed ganoderma lucidum spore powder in the prior art, the wall-removed ganoderma lucidum spore powder has better wall-removed effect and stability. The operation method is simple, quick and efficient, a cup of the wall-removed ganoderma lucidum spore powder drink can be prepared only by tens of seconds, and the effective components in the ganoderma lucidum spore powder can be reserved to the maximum extent, so that the wall-removed ganoderma lucidum spore powder drink has better immunity improving effect compared with the existing wall-removed ganoderma lucidum spore powder product.
Drawings
FIG. 1 is a graph comparing product stability of example 9 and comparative example 2.
FIG. 2 is a graph showing the wall-removing effect of the products of example 9 and comparative example 2.
Description of the embodiments
The technical solutions of the present invention will be clearly and completely described in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The capsule coffee machine used in the present application is a commercially available capsule coffee machine or a capsule coffee machine (including a filter screen) including the basic structure and functions of the capsule coffee machine.
The wall-broken ganoderma lucidum spore powder can be prepared by adopting a conventional wall-breaking process, wherein the wall-broken ganoderma lucidum spore powder is purchased from Zhejiang Zhengzhikang biotechnology Co., ltd, and the capsule cup is purchased from Zheshan national iridescent aluminum foil technology Co., ltd.
Example 1
The capsule coffee machine is applied to removing the wall of the ganoderma lucidum spore powder, and the steps are as follows:
2g of wall-broken ganoderma lucidum spore powder is put into a capsule cup, the mouth of the capsule cup is sealed by an aluminum foil cover, the wall-broken ganoderma lucidum spore powder is put into a hot-melting sealing machine for hot-melting sealing at 200 ℃, after the sealing is tightly confirmed by inspection, the sealed capsule cup containing ganoderma lucidum spore powder is put into a capsule coffee machine, a capsule chamber is closed by pushing and pulling a handle, three extraction needles penetrate through the bottom of the capsule cup, water flows enter the capsule cup through the extraction needles to remove the wall, and the capsule cup sealing film is pushed through to release pressure. Injecting water at 20deg.C, removing wall for 30s under 1bar pressure with water content of 80mL, allowing water flow to flow out through small holes pushed through the sealing membrane, passing through the filter screen to reach the water outlet, and obtaining the final product (wall-removed Ganoderma spore powder liquid) through the water outlet, wherein the obtained wall-removed Ganoderma spore powder liquid can be directly drunk.
Examples 2 to 17
Examples 2-17 tested the effect of different temperatures and pressures on the wall-removing effect, the polysaccharide content and the ganoderma triterpene content in the finished product (wall-removed ganoderma lucidum spore powder liquid). Other parameters were the same as in example 1.
Example 13 differs from example 9 only in that 100g of wall-broken ganoderma lucidum spore powder was placed in a capsule cup.
Example 14 differs from example 9 only in that the wall removal was performed using a derla HV100 capsule coffee machine.
Examples 15-17 differ from example 9 only in that the wall removal times were 60s, 120s, 180s, respectively. Because the water quantity and the time are in a direct proportion, the corresponding water quantity is 160mL, 320mL and 480mL respectively.
The commercial capsule coffee machine is generally free of pressure regulating function, and the pressure-adjustable capsule coffee machine is customized for testing the influence of pressure, and the rest functions are the same as the basic functions of the commercial capsule coffee machine.
The detection modes of polysaccharide content and ganoderma lucidum triterpene content are as follows:
concentrating under reduced pressure and drying to obtain wall-removed Ganoderma spore powder, and measuring polysaccharide content and Ganoderma triterpene content of the wall-removed Ganoderma spore powder.
Polysaccharide content (g/100 g) =polysaccharide content (g) in the wall-removed ganoderma lucidum spore powder/weight of the wall-removed ganoderma lucidum spore powder (g) ×100g
Glossy ganoderma triterpene content (g/100 g) =glossy ganoderma triterpene content (g) in the wall-removed glossy ganoderma spore powder/weight of the wall-removed glossy ganoderma spore powder (g) ×100g.
TABLE 1 polysaccharide and Ganoderma triterpene content in the wall removed Ganoderma spore powder of examples 1-17
As can be seen from examples 1-12, in example 11, the wall is removed for 30s at 100deg.C under 25bar, the wall is removed completely, and after the temperature and pressure are increased, the polysaccharide and Ganoderma triterpene content in the wall removed Ganoderma spore powder has no obvious change.
It can be seen from examples 13 and 9 that the filling amount of the wall-broken ganoderma lucidum spore powder has no obvious effect on the mass content of polysaccharide and ganoderma lucidum triterpene in the wall-broken ganoderma lucidum spore powder.
It can be seen from examples 14 and 9 that the use of different capsule coffee machines has no significant effect on the wall removal effect.
It can be seen from examples 9, 15-17 that increasing the wall removal time and the volume of water has little effect on the wall removal.
Example 18
Two sets of comparative examples were designed in example 18 to compare with the protocol of the present invention.
Comparative example 1
2g of wall-broken ganoderma lucidum spore powder is put into a capsule cup, an aluminum foil cover is put at the mouth of the capsule cup, the capsule cup is put on a hot-melting sealing machine, hot-melting sealing is carried out at 200 ℃, and whether sealing is tight or not is checked. The sealed ganoderma lucidum spore powder capsule is put into a capsule coffee machine to be pricked, then is taken out, is poured for 30s by water with the temperature of 100 ℃ from the pricked position at the bottom by a syringe, and the spore powder flows out from the small hole at the upper part. Concentrating under reduced pressure, and drying. And measuring polysaccharide content and Ganoderma triterpene content respectively.
Comparative example 2 (technical scheme in CN114949009 a)
Filtering Ganoderma spore powder with 100 mesh sieve to remove impurities such as large particle stone and silt, and removing shriveled and silt; thereby improving the purity of the spore powder. Water is used as an extraction solvent, the feed-liquid ratio is 1:20, and the soaking time is as follows: 8h. And (3) pre-dispersing by adopting a high-pressure homogenizer, namely uniformly stirring the ganoderma lucidum spore powder and the solvent, wherein the pressure parameter is set to be 800bar. Emulsifying and breaking the wall of the pre-dispersed ganoderma lucidum spore powder liquid by using a high-pressure homogenizer, setting the parameters to 2000bar, and circulating for 10 times. Filtering the milky ganoderma lucidum spore powder liquid obtained in the wall breaking step, and removing wall shell filter residues; concentrating to obtain concentrated filtrate, centrifuging to obtain oil emulsion layer and extractive solution layer, and centrifuging at 10000r/min for 60min. Concentrating under reduced pressure, and drying to obtain wall-removed Ganoderma spore powder.
The results are shown in Table 2:
TABLE 2 polysaccharide and Ganoderma triterpene content in comparative example products
| Ganoderma lucidum spore powder polysaccharide content g/100g | Ganoderma lucidum triterpene content g/100g | |
| Comparative example 1 | 8.04 | 3.15 |
| Comparative example 2 | 14.62 | 4.19 |
Example 19
This example compares the product stability of the inventive process with that of comparative example 2.
Example 9 and comparative example 2 were allowed to stand together for 1 hour, and the precipitation was observed. The experimental results show that: after standing for 1h, the solution of example 9 remained homogeneous with little precipitation. The upper part of comparative example 2 became clear, the color was more transparent, and there was a large amount of particulate floats; the lower part was cloudy and dark in color, and a large amount of precipitate appeared (see fig. 1). Example 9 and comparative example 2 were placed together in a centrifuge and centrifuged at 2000rpm for 2min. The liquid of the embodiment 9 has uniform color and a small amount of sediment appears on the pipe wall; comparative example 2 the liquid was clear and a large amount of precipitated insoluble material appeared on the tube wall (see fig. 2). Conclusion: the wall removing ganoderma lucidum spore powder by adopting the capsule coffee machine has the advantages that most ganoderma lucidum spore powder walls are removed, and the wall removing effect is better than that of the prior art.
Example 20
ConA induced mouse spleen lymphocyte transformation assay-MTT method:
experimental animals and groupings: SPF grade ICR healthy female mice were selected to be 20, and the weight was 18.0-21.9g, and the mice were randomly divided into two groups of example 9 and comparative example 2 according to the weight, each group being 10 mice. Each mouse had a lavage volume of 50 mL/kg.bw/d, and was divided into 2 lavages on average a day.
After the animals were continuously perfused for 30d, the cervical dislocation method was sacrificed, the spleens were aseptically removed, and the spleens were placed in a plate (60 mm. Times.15 mm) containing an appropriate amount of asepsis Hank's solution and ground to prepare a single spleen cell suspension. After filtration through a 200 mesh screen, the cells were washed 2 times with Hank's solution, centrifuged at 1000r/min for 10min each time, and then suspended in 1mL of RPM 1640 complete medium. Viable cell count (above 95%) was counted by blue-blue staining, and cell concentration was adjusted to 3X 10 with RPMI1640 complete medium 6 And each mL. Adding cell suspension into 24-well culture plate in two wells, 1mL each well, adding ConA solution 75uL at 100ug/mL in one well and ConA solution not in the other well, placing at 37deg.C and 5% CO 2 Culturing in an incubator for 72 hours. 4h before the end of the culture, 0.7mL of supernatant was gently aspirated from each well, 0.7mL of RPMI1640 medium without calf serum was added, and 5mg/mL MTT 50. Mu.L was added to each well, and the culture was continued for 4h. After the cultivation is finished, 1mL of acidic isopropanol is added into each hole, and the mixture is blown and evenly mixed, so that the purple crystals are completely dissolved. The lysate was transferred into 96-well plates, three parallel wells were made for each well, and the optical density values of the individual sample tubes were determined with an enzyme-labeled instrument at a wavelength of 570 nm. The lymph fineness is represented by subtracting the optical density value of the ConA-added wells from the optical density value of the ConA-added wellsThe proliferation capacity of the cells, the optical density difference of the tested sample group is obviously higher than that of the control group, and the positive test result can be judged. The measurement results are shown in the following table 3:
TABLE 3 Table 3
| Group of | Animal number (only) | Difference in absorbance with and without ConA wells |
| Example 9 | 10 | 0.06±0.021 |
| Comparative example 2 | 10 | 0.03±0.024 |
Example 21
DNFB induces delayed type response (DTH) -ear swelling method in mice:
experimental animals and groupings: SPF grade ICR healthy female mice are selected to be 20, the weight is 18.0-21.9g, the mice are randomly divided into two groups of example 9 and comparative example 2 according to the weight, and 10 mice are used in each group. Each mouse had a lavage volume of 50 mL/kg.bw/d, and was divided into 2 lavages on average a day.
After continuous gastric lavage for 30d, the mice were treated on their abdomen Mao Ti with a razor in the range of about 3cm×3cm and sensitized with a 50uL uniform 10mg/mL DNFB solution. After 5d, 10 mu L of DNFB solution with the concentration of 10mg/mL is uniformly smeared on the right ear (two sides) of the mouse for attack, the cervical vertebra dislocation is carried out after 24h of attack to kill the mouse, the left and right earshells are cut off, the earpieces with the diameter of 8mm are taken off by a puncher, and the mouse is weighed. The degree of DTH is expressed as the difference between the weights of the left and right ears. The weight difference of the tested sample group is obviously higher than that of the control group, and the test result can be judged to be positive. The measurement results are shown in the following table 4:
TABLE 4 Table 4
| Group of | Animal number (only) | Ear shell thickening (mg) |
| Example 9 | 10 | 18.1±2.4 |
| Comparative example 2 | 10 | 11.4±6.7 |
Example 22
Antibody-producing cell detection-Jerne modified slide method:
experimental animals and groupings: SPF grade ICR healthy female mice are selected to be 20, the weight is 18.0-21.9g, the mice are randomly divided into two groups of example 9 and comparative example 2 according to the weight, and 10 mice are used in each group. Each mouse had a lavage volume of 50 mL/kg.bw/d, and was divided into 2 lavages on average a day.
After continuous gavage for 30d in each dose group, each mouse was intraperitoneally injected with 2% (v/v) SRBC: immunization was performed with 0.2mL of suspension. After 5d, the cervical dislocation of the mice is killed, the spleens are taken out, and the spleens are put into a small plate containing a proper amount of sterile Hank's liquid for grinding, so as to prepare the cell suspension. Filtering with 200 mesh sieve, centrifuging at 1000r/min for 10min, washing with Hank's liquid for 2 timesFinally, the cells were suspended in 8mL Hank's solution. And heating and dissolving the surface layer culture medium, then, placing the surface layer culture medium in a water bath at 45 ℃ for heat preservation, mixing the surface layer culture medium with equal amount of Hank's liquid with the concentration of 7.2-7.4 twice, and subpackaging small test tubes with 0.5mL of each tube. Then, 50. Mu.L of 10% SRBC suspension (v/v, prepared with SA buffer) and 25. Mu.L of spleen cell suspension were added to the tube, and the mixture was rapidly mixed and poured onto a slide on which a agarose sheet had been brushed, to prepare two parallel plates. After the agar solidifies, the slide is horizontally buckled on a frame and put into 37 ℃ and 5 percent CO 2 Incubate in incubator for 1.5h. Then the prepared complement is diluted by 1:8 and added into a groove of a slide frame, and after incubation is continued for 1.5 hours, the number of hemolysis plaques is counted. The results are expressed by the number of plaques/whole splenocytes, and the number of plaques in the test sample group is significantly higher than that in the control group, and the test results are judged to be positive. The measurement results are shown in the following table 5:
TABLE 5
| Group of | Animal number (only) | Number of lysoplaques (. Times.10) 3 Individual/whole spleen cells) |
| Example 9 | 10 | 16.8±2.5 |
| Comparative example 2 | 10 | 10.4±1.7 |
Example 23
Measurement of serum hemolysin half number hemolysis value (HC) 50 )：
Experimental animals and groupings: SPF grade ICR healthy female mice are selected to be 20, the weight is 18.0-21.9g, the mice are randomly divided into two groups of example 9 and comparative example 2 according to the weight, and 10 mice are used in each group. Each mouse had a lavage volume of 50 mL/kg.bw/d, and was divided into 2 lavages on average a day.
After continuous gastric lavage for 30d, 2% (v/v) SRBC suspension was prepared, each mouse was immunized by intraperitoneal injection with 0.2mL, after 5d, the eyeballs were removed to obtain blood in a centrifuge tube, the tube was left for 1h, centrifuged at 2000r/min for 10min, and serum was isolated and collected. After 200-fold dilution of serum with SA buffer, the optical density of the sample tube and SRBC at half-hemolysis was determined according to the test method in technical specifications. The amount of hemolysin is expressed as a median hemolysis value (HCso), HC in the sample group 50 HC significantly higher than control group 50 The test results were judged positive.
The measurement results are shown in the following table 6:
TABLE 6
| Group of | Animal number (only) | HC 50 |
| Example 9 | 10 | 169.5±32.2 |
| Comparative example 2 | 10 | 92.8±50.3 |
The results of examples 20-23 above show that removal of the Ganoderma lucidum spore powder wall using the capsule coffee machine has a better immunity enhancing effect than directly brewed Ganoderma lucidum spore powder.
It is apparent that the above examples are given by way of illustration only and are not limiting of the embodiments. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. While obvious variations or modifications are contemplated as falling within the scope of the present invention.
Claims (10)
1. A method for removing the spore powder wall of ganoderma lucidum, which is characterized by comprising the following steps:
filling the wall-broken ganoderma lucidum spore powder into a capsule cup, sealing, and then placing into a capsule coffee machine;
the extraction needle pierces the capsule cup, high-pressure water flow enters the capsule cup through the extraction needle to remove the wall, the water flow pushes through the capsule sealing membrane and flows out through the filter screen to obtain wall-removed ganoderma lucidum spore powder liquid;
wherein the water flow pressure is more than or equal to 1bar, the water temperature is more than or equal to 20 ℃, and the wall removing time is less than 5min.
2. The method according to claim 1, wherein the water stream pressure is 1-30bar and the water temperature is 20-110 ℃.
3. The method of claim 1, wherein the wall removal time is less than or equal to 1 minute.
4. The method according to claim 1, wherein after the wall-broken ganoderma lucidum spore powder is added into the capsule cup, the mouth of the capsule cup is sealed by an aluminum foil cover, and the capsule cup is placed on a hot-melting sealing machine for hot-melting sealing.
5. The application of the capsule coffee machine in removing the wall of ganoderma lucidum spore powder.
6. The use according to claim 5, wherein the wall-broken ganoderma lucidum spore powder is added into a capsule cup, and then the capsule cup is put into a capsule coffee machine, and the wall is removed for less than 5 minutes under the conditions that the water temperature is more than or equal to 1bar and the water temperature is more than or equal to 20 ℃ to obtain the wall-removed ganoderma lucidum spore powder liquid.
7. The use according to claim 6, wherein the wall is removed at 20-110 ℃ and 1-30 bar.
8. The method according to claim 6, wherein the wall removal time is less than 1min.
9. The method according to claim 6, wherein after the wall-broken ganoderma lucidum spore powder is added into the capsule cup, the mouth of the capsule cup is sealed by an aluminum foil cover, and the capsule cup is placed on a hot-melting sealing machine for hot-melting sealing.
10. A wall-removed ganoderma lucidum spore powder drink is characterized in that wall-broken ganoderma lucidum spore powder is added into a capsule cup, and then the capsule cup is put into a capsule coffee machine, and the wall is removed for less than 5 minutes under the conditions that the water temperature is more than or equal to 1bar and the water temperature is more than or equal to 20 ℃.
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