CN116162547A - Strain fermentation incubator and use method - Google Patents

Strain fermentation incubator and use method Download PDF

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Publication number
CN116162547A
CN116162547A CN202310184134.2A CN202310184134A CN116162547A CN 116162547 A CN116162547 A CN 116162547A CN 202310184134 A CN202310184134 A CN 202310184134A CN 116162547 A CN116162547 A CN 116162547A
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culture
adjusting
drums
incubator
groups
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宋学磊
宋学丽
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Zhucheng Shunwo Agricultural Technology Co ltd
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Zhucheng Shunwo Agricultural Technology Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/10—Rotating vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability

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Abstract

The invention provides a strain fermentation incubator and a use method thereof, which relate to the field of strain culture and comprise the following steps: the device comprises an adjusting disc, a first adjusting rod, a first transition shaft, a second adjusting rod, a second transition shaft, a lower adjusting shaft, a culture cylinder, a culture dish, a metal ring and a rotary driving gear; the outer ring end of the adjusting disk is provided with an outer ring groove and is rotationally clamped in a through hole of the upper partition board through the outer ring groove, the lower adjusting shaft is rotationally inserted on the lower partition board, the outer end of the disk at the top end is connected with four groups of outer connecting rods, the outer ends of the outer connecting rods are respectively connected with an outer inserting ring, the culture drums are respectively inserted in the outer inserting rings, the culture dishes are installed in the culture drums through clamping grooves and guide clamping rails in the outer sides of the culture drums in an inserting mode, four groups of metal rings are arranged, and the rotary driving gear is arranged at the left end of the upper side of the lower partition board. The test phage is in the state of different temperatures and different motion culture, and solves the problem that a single culture device cannot independently regulate the temperature of the culture dish because the culture dish is taken out from the culture device for observation.

Description

Strain fermentation incubator and use method
Technical Field
The invention relates to the technical field of strain culture, in particular to a strain fermentation incubator and a use method thereof.
Background
Phage (phage) is a virus that attacks bacteria, and is also genetic material that confers biological traits on host bacteria. In the phage test, phage culture is required.
In the current phage culture device, the culture dish needs to be taken out from the culture device for observation in the culture, which is troublesome and affects the culture process, and secondly, the temperature of the internal culture dish cannot be independently regulated by a single culture device, so that the culture needs to be carried out in different culture devices, and the cost is increased.
Disclosure of Invention
In view of this, the present invention provides a strain fermentation incubator and a method of use that is capable of testing the state of a phage under different temperature cultures and the state of the test phage under different movements, and also capable of adjusting and changing the magnification of an objective lens within the incubator.
The invention provides a strain fermentation incubator and a use method thereof, and particularly comprises an incubator main body, an adjusting disc, a first adjusting rod, a first transition shaft, a second adjusting rod, a second transition shaft, a lower adjusting shaft, an incubator barrel, a culture dish, a metal ring and a rotary driving gear;
the utility model provides a culture dish, including culture dish, outer ring groove, first regulation pole and first transition axle, the culture dish is equipped with the baffle, the upper baffle upside in the culture dish is installed in the rotation respectively, and the top of first regulation pole upwards wears out the culture dish main part and installs the operation hand wheel, the second regulation pole is rotated respectively with the second transition axle and is installed in the lower baffle downside in the culture dish main part, and the top of second regulation pole upwards wears out the culture dish main part and also installs the operation hand wheel, the lower regulating axle rotates and alternates on the lower baffle, the disc outer end on top is connected with four sets of outer connecting rods, and the outer end of outer connecting rod is connected with the outer ring respectively, the culture dish is transparent structure, is equipped with four sets of and inserts respectively in the outer ring groove, and is connected with the culture dish on the outside, the culture dish is equipped with the guide rail side of rotation motor, the guide rail is equipped with the counter-drive motor, the counter-drive motor is equipped with the metal rail, the counter-drive motor is equipped with the arc in the side of counter-drive motor, and the outer side of counter-drive motor is equipped with the arc.
Optionally, four groups of mounting holes are further formed in the adjusting disc, objective lenses are inserted and clamped in the four groups of mounting holes respectively, and the bottom end of the lens cone is opposite to the objective lenses of the front side group up and down.
Optionally, first adjusting gear is installed to the lower extreme of first regulation pole, and first linkage gear is installed to the lower extreme of first transition axle, is equipped with 1/4 latch on the first adjusting gear to with first linkage gear meshing, and first adjusting gear is 4:1 with first linkage gear's transmission ratio, still be equipped with the regulating axle in the middle of the top side of regulating disk, first transition axle is connected with the regulating axle transmission.
Optionally, a second adjusting gear is installed at the lower end of the second adjusting rod, a second linkage gear is installed at the lower end of the second transition shaft, 1/4 latch teeth are arranged on the second adjusting gear and meshed with the second linkage gear, the transmission ratio of the second adjusting gear to the second linkage gear is 4:1, and the second transition shaft is in transmission connection with the lower adjusting shaft.
Optionally, four groups the inboard bottom of cultivateing the section of thick bamboo is inlayed respectively and is had the balancing weight, and is hollow structure respectively in the middle of the section of thick bamboo circle of four groups cultivateing the section of thick bamboo to install spiral heater strip, spiral heater strip links to each other with the side direction V type guide piece of cultivateing the section of thick bamboo bottom respectively, and cultivates when the section of thick bamboo rotates to the metal circle upside, side direction V type guide piece contacts with the metal circle.
Optionally, the top ends of the four groups of culture cylinders are also respectively connected with a sealing cover, and the sealing covers are also embedded with a temperature sensor and a temperature display.
Optionally, the outer rings of the four groups of culture cylinders are respectively provided with rotary teeth, and when the centers of the culture cylinders of the front group are opposite to the centers of the through holes from top to bottom, the rotary teeth of the culture cylinders of the left group are meshed with the rotary driving gear.
Optionally, the method for using the strain fermentation incubator is as follows:
1) Opening a front side door of the incubator, respectively inserting and installing a culture dish filled with bacteriophage into the culture cylinder through a clamping groove and a guide clamping rail at the outer side of the culture dish, respectively installing sealing covers at the top ends of the culture cylinder, respectively inserting the culture cylinder into an outer inserting ring, and inserting and clamping objective lenses with different multiples into mounting holes of an adjusting disc;
2) The lateral V-shaped guide piece at the bottom of the culture cylinder is contacted with the metal ring, so that a power supply of the incubator body is communicated with the spiral heating wire, the dishes in the culture cylinder are heated respectively, the work of the spiral heating wire is controlled by a switch respectively, and the temperature in the culture cylinder is mastered by a temperature sensor and a temperature display, so that different temperatures in two adjacent culture cylinders in four groups of culture cylinders and the same temperature in two opposite culture cylinders can be regulated respectively, and the state of the phage cultured at different temperatures is tested;
3) The microscope is formed by using the inserted lens cone, ocular lens and objective lenses which are vertically opposite to the bottom side of the lens cone, phage in a culture dish in a culture cylinder at the bottom side of the microscope is observed, and a first transition shaft and an adjusting disc are driven to rotate 1/4 circle by rotating a first adjusting rod 1 circle, so that the other group of objective lenses are rotated to the positive bottom side of the lens cone, and the multiple of the objective lenses is adjusted in a incubator;
4) The rotary driving gear is driven by the driving motor to rotate, and is meshed with the rotary teeth of the culture cylinders of the left group to drive the culture cylinders of the left group to rotate, so that the culture dish can rotate along with the culture cylinders, the other opposite culture cylinder rotating to the left group is not driven by the rotary driving gear, and the bacteriophage is tested in the state of the same temperature and different movements;
5) And the second transition shaft and the lower adjusting shaft are driven to rotate 1/4 circle by rotating the second adjusting rod for 1 circle, so that the other group of culture cylinders are rotated to the through holes, and phage in the culture dishes in the four groups of culture cylinders are respectively observed through a microscope.
Advantageous effects
The invention can test the state of the phage under different temperature culture and the state of the test phage under different movement, and can also adjust and change the multiple of the objective lens in the incubator.
In addition, through setting up four groups of cultivation barrels to inlay four groups of metal rings on lower baffle, when the side direction V type guide blade of cultivation barrel bottom contacted with the metal ring, make the power and the spiral heater wire of cultivate the ware main part communicate with each other, can heat the culture dish in the cultivation barrel respectively, utilize the work of switch control spiral heater wire respectively, and utilize temperature sensor and temperature display to master the temperature in the cultivation barrel, thereby can adjust the different temperatures in two sets of adjacent cultivation barrels and the same temperature in two sets of relative cultivation barrels in four groups of cultivation barrels respectively, so that can test the state of phage under different temperature cultivation.
In addition, through four sets of objective of cartridge on the regulating disk, utilize the lens cone of cartridge, eyepiece and lens that the lens cone bottom side is relative from top to bottom to constitute the microscope, so that can observe the phage in the culture dish in the culture section of thick bamboo of its bottom side, the first latch that adjusts the pole of accessible rotation drives on the first adjusting gear meshes with the first linkage gear on the first transition axle, drive first transition axle and rotate, and then drive the regulating disk and rotate, because the transmission ratio of first adjusting gear and first linkage gear is 4:1, when first adjusting pole rotates 1 round, and then drive first transition axle and regulating disk and rotate 1/4 circle, thereby make another set of objective rotate to the lens cone positive bottom side, thereby be convenient for adjust the multiple of objective in the incubator, more do benefit to experimental observation.
In addition, through inserting four groups of culture drums respectively in the outer inserting ring outside the disc at the top of the lower adjusting shaft, the second adjusting rod can be rotated to drive the latch on the second adjusting gear to be meshed with the second linkage gear on the second transition shaft, the second transition shaft is driven to rotate, and then the lower adjusting shaft is driven to rotate, and when the second adjusting rod rotates for 1 circle, the second transition shaft and the lower adjusting shaft are driven to rotate for 1/4 circle, so that the other group of culture drums are rotated to the through holes, and phage in culture dishes in the four groups of culture drums are observed respectively through a microscope.
In addition, through setting up driving motor and rotation driving gear to set up rotatory tooth at the outside end of cultivateing the section of thick bamboo, when the center of cultivateing the section of thick bamboo of front side group and the center of thru hole are relative from top to bottom, the rotatory tooth of the cultivateing the section of thick bamboo of left side group and rotation driving gear meshing, accessible driving motor drives rotation driving gear and rotates, and then drives the cultivateing section of thick bamboo of left side group and rotate, because the culture dish passes through draw-in groove cartridge inside cultivateing the section of thick bamboo, thereby can make the culture dish follow and cultivate the section of thick bamboo rotation, thereby better experimental phage is in the state under the different motions.
Drawings
In order to more clearly illustrate the technical solution of the embodiments of the present invention, the drawings of the embodiments will be briefly described below.
The drawings described below are only for illustration of some embodiments of the invention and are not intended to limit the invention.
In the drawings:
FIG. 1 shows a schematic diagram of the overall structure according to an embodiment of the present invention;
FIG. 2 is a schematic view showing a structure of the incubator body of FIG. 1 after the front side door is opened according to an embodiment of the present invention;
FIG. 3 is a schematic view showing the structure of the front side door of FIG. 2 after disassembly, according to an embodiment of the present invention;
fig. 4 is a schematic view showing a structure of the lens barrel and the eyepiece of fig. 3 when the lens barrel and the eyepiece are pulled up according to an embodiment of the invention;
FIG. 5 shows an enlarged schematic view of the structure of FIG. 4 at A in accordance with an embodiment of the invention;
FIG. 6 is a schematic view showing a structure in which the culture cartridges of the front side group of FIG. 4 are drawn upward according to an embodiment of the present invention;
FIG. 7 is an enlarged schematic view of the structure of FIG. 6 at B according to an embodiment of the present invention;
FIG. 8 illustrates a schematic diagram of the lean-side configuration of FIG. 6, in accordance with an embodiment of the present invention;
FIG. 9 is an enlarged schematic view of the structure of FIG. 8 at C according to an embodiment of the present invention;
FIG. 10 is a schematic view showing the structure of the actuator disk and four objective lenses of FIG. 6 drawn out of the actuator disk according to an embodiment of the present invention;
FIG. 11 is a schematic view showing a structure in which a sealing cover is removed upward from a culture drum in FIG. 6 according to an embodiment of the present invention;
FIG. 12 illustrates a schematic diagram of the lean-side configuration of FIG. 11, in accordance with an embodiment of the present invention;
FIG. 13 is a schematic view showing the structure of the culture dish of FIG. 11 after being taken up and one end of the culture drum being cut away according to an embodiment of the present invention.
List of reference numerals
1. A incubator body; 101. an upper partition plate; 102. a lower partition plate; 1021. a through hole; 103. a jack; 201. a lens barrel; 202. an eyepiece; 203. an objective lens; 3. an adjusting plate; 301. an outer ring groove; 302. a mounting hole; 303. an adjusting shaft; 401. a first adjusting lever; 4011. a first adjusting gear; 402. a first transition shaft; 4021. a first linkage gear; 403. a second adjusting lever; 4031. a second adjusting gear; 404. a second transition shaft; 4041. a second linkage gear; 5. a lower adjustment shaft; 501. an outer link; 502. an outer insert ring; 6. a culture drum; 601. an arc-shaped plate; 602. a guide clamping rail; 603. balancing weight; 604. a spiral heating wire; 605. lateral V-shaped guide vanes; 606. sealing cover; 6061. a temperature display; 607. rotating the teeth; 7. a culture dish; 701. a clamping groove; 8. a metal ring; 9. the drive gear is rotated.
Detailed Description
In order to make the objects, aspects and advantages of the technical solution of the present invention more clear, the technical solution of the embodiment of the present invention will be clearly and completely described below with reference to the accompanying drawings of the specific embodiment of the present invention. Unless otherwise indicated, terms used herein have the meaning common in the art. Like reference numerals in the drawings denote like parts.
Examples: please refer to fig. 1 to 13:
the invention provides a strain fermentation incubator and a use method thereof, comprising the following steps: the incubator body 1, the adjusting disk 3, the first adjusting lever 401, the first transition shaft 402, the second adjusting lever 403, the second transition shaft 404, the lower adjusting shaft 5, the incubator barrel 6, the culture dish 7, the metal ring 8 and the rotation driving gear 9;
the whole incubator body 1 is of a transparent structure, an upper partition plate 101 and a lower partition plate 102 are arranged in the incubator body 1, an insertion hole 103 is formed in the front end of the top side of the incubator body 1, a lens barrel 201 is inserted and clamped, an ocular 202 is inserted into the top of the lens barrel 201, an outer ring groove 301 is formed in the outer ring end of an adjusting disc 3 and is rotationally clamped in a through hole of the upper partition plate 101 through the outer ring groove 301, a first adjusting rod 401 and a first transition shaft 402 are respectively rotationally arranged on the upper side of the upper partition plate 101 in the incubator body 1, the top end of the first adjusting rod 401 upwards penetrates out of the incubator body 1 and is provided with an operating hand wheel, a second adjusting rod 403 and a second transition shaft 404 are respectively rotationally arranged on the lower side of the lower partition plate 102 in the incubator body 1, the top end of the second adjusting rod 403 upwards penetrates out of the incubator body 1 and is also provided with the operating hand wheel, and the lower adjusting shaft 5 is rotationally inserted on the lower partition plate 102, the outer end of the disk at the top end is connected with four groups of outer connecting rods 501, the outer ends of the outer connecting rods 501 are respectively connected with an outer inserting ring 502, the culture cylinder 6 is of a transparent structure and provided with four groups and is respectively inserted in the outer inserting ring 502, the outer side of the culture cylinder 6 is connected with an arc plate 601, serial numbers are arranged on the arc plate 601, two groups of opposite guide clamping rails 602 are also arranged in the culture cylinder 6, the culture dish 7 is inserted and installed in the culture cylinder 6 through a clamping groove 701 at the outer side of the culture dish 7 and the guide clamping rails 602, four groups of metal rings 8 are arranged, the front side of the culture dish 7 is assembled outside a through hole 1021 on the lower partition plate 102, the other three groups of metal rings 8 are annularly arranged with an adjusting shaft 5 below the front side group of metal rings 8 as the center, the four groups of metal rings 8 are respectively connected with a power supply of the culture main body 1 through a switch, a rotary driving gear 9 is arranged at the left end at the upper side of the lower partition plate 102, a driving motor is also arranged in a bottom end cavity of the culture main body 1, and the rotary driving gear 9 is connected with a motor shaft of the driving motor through a rotating shaft.
As shown in fig. 4, fig. 5 and fig. 10, four groups of mounting holes 302 are further formed in the adjusting disk 3, the objective 203 is inserted and clamped in each mounting hole, the bottom end of the lens barrel 201 is opposite to the objective 203 of the front side group up and down, and then the lens barrel 201, the eyepiece 202 and the objective 203 which are opposite up and down form a microscope, so that phage in the culture dish 7 in the culture drum 6 at the bottom side of the microscope can be observed, a first adjusting gear 4011 is mounted at the lower end of the first adjusting rod 401, a first linkage gear 4021 is mounted at the lower end of the first transition shaft 402, 1/4 latch is arranged on the first adjusting gear 4011, and is meshed with the first linkage gear 4021, the transmission ratio of the first adjusting gear 4011 to the first linkage gear 4021 is 4:1, the first transition shaft 402 is in transmission connection with the adjusting shaft 303, and the first transition shaft 401 is driven to rotate by rotating the first adjusting rod 401 to drive the latch on the first adjusting gear 4011 to be meshed with the first gear 4021 on the first transition shaft 402, and then the first transition shaft 402 is driven to rotate the first transition shaft 402 to rotate to the first linkage gear 4021, and then the first linkage gear 203 is driven to rotate to the first transition shaft 1:4, and then the objective 203 is driven to rotate to the first transition shaft 1 is in rotation to rotate to the first transition gear 1.
As shown in fig. 8 and 9, a second adjusting gear 4031 is installed at the lower end of the second adjusting rod 403, a second linkage gear 4041 is installed at the lower end of the second transition shaft 404, 1/4 latch is provided on the second adjusting gear 4031 and meshed with the second linkage gear 4041, the transmission ratio of the second adjusting gear 4031 to the second linkage gear 4041 is 4:1, the second transition shaft 404 is in transmission connection with the lower adjusting shaft 5, the latch on the second adjusting gear 4031 is meshed with the second linkage gear 4041 on the second transition shaft 404 by rotating the second adjusting rod 403, the second transition shaft 404 is driven to rotate, and then the lower adjusting shaft 5 is driven to rotate, and as the transmission ratio of the second adjusting gear 4031 to the second linkage gear 4041 is 4:1, when the second adjusting rod 403 rotates for 1 turn, the second transition shaft 404 and the lower adjusting shaft 5 are driven to rotate for 1/4 turn, so that the other group of culture drums 6 rotates to the through holes, and the phages in the culture drums 7 in the four groups of culture drums 6 are observed by a microscope 1021 respectively.
As shown in fig. 6, fig. 7, fig. 11 and fig. 13, the inside bottom ends of the four groups of culture drums 6 are respectively embedded with balancing weights 603, hollow structures are respectively arranged in the middle of the drum circles of the four groups of culture drums 6, spiral heating wires 604 are respectively connected with lateral V-shaped guide plates 605 at the bottoms of the culture drums 6, when the culture drums 6 rotate to the upper sides of the metal circles 8, the lateral V-shaped guide plates 605 are contacted with the metal circles 8, the top ends of the four groups of culture drums 6 are respectively connected with sealing covers 606, the sealing covers 606 are also embedded with temperature sensors and temperature displays 6061, the sealing covers 606 are also transparent structures, and by arranging the balancing weights 603 at the inside bottom ends of the culture drums 6, the bottoms of the culture drums 6 can be better attached to the lower partition plates 102 under the action of the balancing weights 603, the lateral V-shaped guide plates 605 can be in contact with the metal circles 8 to form conduction, power supplies of the culture drum bodies 1 are communicated with the spiral heating wires 604, the culture drums 7 in the culture drums 6 are respectively heated, the work of the spiral heating wires 604 is respectively controlled by using switches, the temperature sensors are respectively used for controlling the operation of the spiral heating wires 604, the temperature sensors and the temperature sensors are used for displaying the temperatures of the temperature sensors 6061, the temperature sensors in the two groups of the culture drums 6 can be different from the two groups of culture drums 6, and the temperature of the same temperature conditions can be different than those in the two groups of adjacent culture drums 6 are different, and different in the culture drums are different in temperature conditions and different in conditions can be different in the culture drum conditions and different in temperature conditions and different conditions.
As shown in fig. 4, the outer rings of the four groups of culture drums 6 are respectively provided with a rotary tooth 607, when the centers of the culture drums 6 of the front group are opposite to the centers of the through holes 1021 up and down, the rotary tooth 607 of the culture drum 6 of the left group is meshed with the rotary driving gear 9, the rotary driving gear 9 can be driven to rotate by the driving motor, the rotary driving gear 9 is meshed with the rotary tooth 607 of the culture drum 6 of the left group to drive the culture drum 6 of the left group to rotate, so that the culture dish 7 can rotate along with the culture drum 6, and the opposite culture drum 6 which is turned to the left group is not driven by the rotary driving gear 9, and the test phage is in the state of the same temperature and different motions.
Specific use and action of the embodiment: firstly, opening a front side door of a incubator, respectively inserting and installing a culture dish 7 filled with bacteriophage into a culture cylinder 6 through a clamping groove 701 and a guide clamping rail 602 at the outer side of the culture dish, respectively installing a sealing cover 606 at the top end of the culture cylinder 6, respectively inserting the culture cylinder 6 into an outer inserting ring 502, and inserting and clamping objective lenses 203 with different multiples into mounting holes of an adjusting disk 3; the lateral V-shaped guide sheet 605 at the bottom of the culture cylinder 6 is contacted with the metal ring 8, so that the power supply of the incubator main body 1 is communicated with the spiral heating wire 604, the petri dishes 7 in the culture cylinder 6 are respectively heated, the work of the spiral heating wire 604 is controlled by a switch, and the temperature in the culture cylinder is mastered by a temperature sensor and a temperature display 6061, thereby respectively adjusting the different temperatures in two adjacent culture cylinders 6 in four groups of culture cylinders 6 and the same temperature in two opposite culture cylinders 6, and testing the culture state of phage under different temperatures; the microscope is formed by the inserted lens cone 201, the ocular 202 and the objective 203 which is opposite up and down at the bottom side of the lens cone 201, phage in a culture dish 7 in a culture cylinder 6 at the bottom side of the microscope is observed, a first adjusting rod 401 is rotated to drive a latch on a first adjusting gear 4011 to be meshed with a first linkage gear 4021 on a first transition shaft 402, the first transition shaft 402 is driven to rotate, and then an adjusting disc 3 is driven to rotate, and as the transmission ratio of the first adjusting gear 4011 to the first linkage gear 4021 is 4:1, when the first adjusting rod 401 rotates for 1 circle, the first transition shaft 402 and the adjusting disc 3 are driven to rotate for 1/4 circle, so that the other group of objective 203 rotates to the positive bottom side of the lens cone 201, and the multiple of the objective 203 is adjusted in the incubator; the rotary driving gear 9 can be driven to rotate by the driving motor, the rotary driving gear 9 is meshed with the rotary teeth 607 of the left group of culture drums 6 to drive the left group of culture drums 6 to rotate, so that the culture dish 7 can rotate along with the culture drums 6, the other opposite culture drum 6 rotating to the left group is not driven by the rotary driving gear 9, and the bacteriophage is tested in the same temperature and different motion states; the second adjusting rod 403 is rotated to drive the latch on the second adjusting gear 4031 to be meshed with the second linkage gear 4041 on the second transition shaft 404, so as to drive the second transition shaft 404 to rotate and further drive the lower adjusting shaft 5 to rotate, and as the transmission ratio of the second adjusting gear 4031 to the second linkage gear 4041 is 4:1, when the second adjusting rod 403 rotates for 1 circle, the second transition shaft 404 and the lower adjusting shaft 5 are further driven to rotate for 1/4 circle, so that the other group of culture drums 6 rotate to the through holes 1021, and the phages in the culture dishes 7 in the four groups of culture drums 6 are respectively observed through a microscope.
The foregoing is merely exemplary embodiments of the present invention and is not intended to limit the scope of the invention, which is defined by the appended claims.

Claims (8)

1. A strain fermentation incubator, comprising: the incubator comprises an incubator body (1), an adjusting disc (3), a first adjusting rod (401), a first transition shaft (402), a second adjusting rod (403), a second transition shaft (404), a lower adjusting shaft (5), an incubator barrel (6), a petri dish (7), a metal ring (8) and a rotary driving gear (9);
the whole incubator body (1) is of a transparent structure, an upper partition plate (101) and a lower partition plate (102) are arranged in the incubator body (1), an inserting hole (103) is formed in the front end of the top side of the incubator body (1), a lens barrel (201) is inserted in the inserting hole, an ocular lens (202) is inserted in the top of the lens barrel (201), an outer ring groove (301) is formed in the outer ring end of the adjusting disc (3), the ocular lens is rotatably clamped in a through hole of the upper partition plate (101) through the outer ring groove (301), the first adjusting rod (401) and the first transition shaft (402) are respectively rotatably mounted on the upper side of the upper partition plate (101) in the incubator body (1), the top end of the first adjusting rod (401) upwards penetrates out of the incubator body (1) and is provided with an operating hand wheel, the second adjusting rod (403) and the second transition shaft (404) are respectively rotatably mounted on the lower side of the lower partition plate (102) in the incubator body (1), the top end of the second adjusting rod (403) upwards penetrates out of the incubator body (1) and is also provided with an operating hand wheel, the lower adjusting shaft (5) is rotatably mounted on the upper partition plate (101) and is respectively provided with an outer connecting rod (502), the outer connecting rod (502) is respectively inserted in the outer end of the incubator body (6), and cultivate the outside of section of thick bamboo (6) and be connected with arc (601), be equipped with the serial number on arc (601), still be equipped with two sets of relative direction card rail (602) in cultivateing section of thick bamboo (6), and cultivate dish (7) through draw-in groove (701) and direction card rail (602) in its outside insert the installation in cultivateing section of thick bamboo (6), ringlet (8) are equipped with four groups, and the front side group is established in the thru hole (1021) outside on baffle (102) down, and three groups ringlet (8) are the annular with front side group ringlet (8) below (5) as the center and are the annular arrangement, and four groups ringlet (8) link to each other through the switch with the power of cultivateing ware main part (1) respectively, rotary drive gear (9) are established in the bottom intracavity of baffle (102) upside left end down, and link to each other through the pivot between rotary drive gear (9) and driving motor's the motor shaft.
2. A strain fermentation incubator as claimed in claim 1, wherein: four groups of mounting holes (302) are further formed in the adjusting disc (3), the objective lenses (203) are inserted and clamped in the four groups of mounting holes respectively, and the bottom ends of the lens barrels (201) are opposite to the objective lenses (203) of the front side groups up and down.
3. A strain fermentation incubator as claimed in claim 1, wherein: the lower extreme of first regulation pole (401) is installed first adjusting gear (4011), and first linkage gear (4021) is installed to the lower extreme of first transition axle (402), is equipped with 1/4 latch on first adjusting gear (4011) to with first linkage gear (4021) meshing, and the transmission ratio of first adjusting gear (4011) and first linkage gear (4021) is 4:1, still be equipped with regulating axle (303) in the middle of the top side of regulating disk (3), first transition axle (402) are connected with regulating axle (303) transmission.
4. A strain fermentation incubator as claimed in claim 1, wherein: the lower end of the second adjusting rod (403) is provided with a second adjusting gear (4031), the lower end of the second transition shaft (404) is provided with a second linkage gear (4041), the second adjusting gear (4031) is provided with 1/4 latch teeth and is meshed with the second linkage gear (4041), the transmission ratio of the second adjusting gear (4031) and the second linkage gear (4041) is 4:1, and the second transition shaft (404) is in transmission connection with the lower adjusting shaft (5).
5. A strain fermentation incubator as claimed in claim 1, wherein: the balancing weights (603) are respectively embedded at the bottom ends of the inner sides of the four groups of culture drums (6), hollow structures are respectively arranged in the middle of the drum circles of the four groups of culture drums (6), spiral heating wires (604) are arranged, the spiral heating wires (604) are respectively connected with lateral V-shaped guide pieces (605) at the bottoms of the culture drums (6), and when the culture drums (6) rotate to the upper sides of the metal circles (8), the lateral V-shaped guide pieces (605) are in contact with the metal circles (8).
6. A strain fermentation incubator as recited in claim 5 wherein: the top ends of the four groups of culture drums (6) are also respectively connected with a sealing cover (606), and the sealing covers (606) are also embedded with a temperature sensor and a temperature display (6061).
7. A strain fermentation incubator as claimed in claim 6, wherein: the outer rings of the four groups of culture drums (6) are respectively provided with rotary teeth (607), and when the centers of the culture drums (6) of the front group are opposite to the centers of the through holes (1021) up and down, the rotary teeth (607) of the culture drums (6) of the left group are meshed with the rotary driving gear (9).
8. A method of using a seed fermentation incubator as claimed in claims 1-7, wherein: the using method is as follows:
1), opening a front side door of a incubator, respectively inserting and installing a culture dish (7) filled with bacteriophage into a culture cylinder (6) through a clamping groove (701) and a guide clamping rail (602) at the outer side of the culture dish, respectively installing sealing covers (606) at the top end of the culture cylinder (6), respectively inserting the culture cylinder (6) into an outer inserting ring (502), and inserting and clamping objective lenses (203) with different multiples into mounting holes of an adjusting disc (3);
2) The lateral V-shaped guide piece (605) at the bottom of the culture cylinder (6) is contacted with the metal ring (8), so that the power supply of the incubator main body (1) is communicated with the spiral heating wire (604), the petri dishes (7) in the culture cylinder (6) are respectively heated, the work of the spiral heating wire (604) is respectively controlled by a switch, and the temperature in the culture cylinder is mastered by a temperature sensor and a temperature display (6061), so that the different temperatures in two adjacent culture cylinders (6) in four groups of culture cylinders (6) and the same temperature in two opposite culture cylinders (6) can be respectively regulated, and the state of the test phage under the culture of different temperatures can be tested;
3) The microscope is composed of a lens cone (201), an ocular lens (202) and objective lenses (203) which are arranged in an inserted manner and are vertically opposite to each other on the bottom side of the lens cone (201), phage in a culture dish (7) in a culture cylinder (6) on the bottom side of the microscope is observed, a first transition shaft (402) and an adjusting disc (3) are driven to rotate 1/4 circle by rotating a first adjusting rod (401) for 1 circle, so that the other group of objective lenses (203) are rotated to the positive bottom side of the lens cone (201), and the multiple of the objective lenses (203) is adjusted in the culture device;
4) The rotary driving gear (9) can be driven to rotate by the driving motor, the rotary driving gear (9) is meshed with the rotary teeth (607) of the culture drums (6) of the left group to drive the culture drums (6) of the left group to rotate, so that the culture dishes (7) can rotate along with the culture drums (6), and the other opposite culture drums (6) rotating to the left group are not driven by the rotary driving gear (9), and test phage are in the states of the same temperature and different motions;
5) And the second transition shaft (404) and the lower adjusting shaft (5) are driven to rotate for 1/4 circle by rotating the second adjusting rod (403) for 1 circle, so that the other group of culture drums (6) are rotated to the through holes (1021), and phage in the culture dishes (7) in the four groups of culture drums (6) are observed through a microscope respectively.
CN202310184134.2A 2023-03-01 2023-03-01 Strain fermentation incubator and use method Pending CN116162547A (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111575170A (en) * 2020-06-18 2020-08-25 李鑫 Microorganism detection device capable of performing continuous operation detection
CN211814496U (en) * 2020-02-15 2020-10-30 青岛农业大学 Visual observation embryo development's device
CN112897690A (en) * 2021-02-05 2021-06-04 诸城舜沃农业科技有限公司 Anaerobic fermentation device capable of being switched randomly based on one-stage two-stage fermentation
CN113388497A (en) * 2021-04-28 2021-09-14 叶鹏超 Culture apparatus for observing growth process of biological stem cells in each period in classified manner
CN215935765U (en) * 2021-05-19 2022-03-04 福建成发农业开发有限公司 Edible mushroom strain cultivation device
CN114395479A (en) * 2022-01-24 2022-04-26 莆田学院 Stem cell biological growth observation device for rectal adenocarcinoma treatment
CN217717513U (en) * 2022-06-07 2022-11-01 广检检测技术(上海)有限公司 Bactericidal effect detection device

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN211814496U (en) * 2020-02-15 2020-10-30 青岛农业大学 Visual observation embryo development's device
CN111575170A (en) * 2020-06-18 2020-08-25 李鑫 Microorganism detection device capable of performing continuous operation detection
CN112897690A (en) * 2021-02-05 2021-06-04 诸城舜沃农业科技有限公司 Anaerobic fermentation device capable of being switched randomly based on one-stage two-stage fermentation
CN113388497A (en) * 2021-04-28 2021-09-14 叶鹏超 Culture apparatus for observing growth process of biological stem cells in each period in classified manner
CN215935765U (en) * 2021-05-19 2022-03-04 福建成发农业开发有限公司 Edible mushroom strain cultivation device
CN114395479A (en) * 2022-01-24 2022-04-26 莆田学院 Stem cell biological growth observation device for rectal adenocarcinoma treatment
CN217717513U (en) * 2022-06-07 2022-11-01 广检检测技术(上海)有限公司 Bactericidal effect detection device

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