CN113388497A

CN113388497A - Culture apparatus for observing growth process of biological stem cells in each period in classified manner

Info

Publication number: CN113388497A
Application number: CN202110464445.5A
Authority: CN
Inventors: 叶鹏超
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-04-28
Filing date: 2021-04-28
Publication date: 2021-09-14

Abstract

The invention provides a culture device for observing growth processes of biological stem cells in each period in a classified manner, which comprises a culture box, a threaded rod, a connecting rod and a culture dish; the lower end of the incubator is fixedly connected with the H-shaped supporting plate, the right side of the upper end of the incubator is provided with a movable cover plate, and the left side of the upper end of the incubator is provided with an observation cylinder; the threaded rod penetrates through the H-shaped supporting plate and is fixedly connected with the hand wheel; the upper end of the connecting rod is fixedly connected with the bottom plate of the incubator, and the lower end of the connecting rod sequentially penetrates through the limiting disc and the rotating rod and is fixedly connected with the driven bevel gear; the side plate of the incubator is provided with a liquid conveying pipe, and the upper end of the liquid conveying pipe is provided with a feeding hole. According to the invention, the sliding plate is slidably clamped in the limiting groove, so that when the culture dish is placed in the groove, the culture dish extrudes the fixture block into the limiting groove, the compression spring is stressed and compressed to provide a reaction force for the sliding plate, the fixture block at the front end of the sliding plate is tightly attached to the culture dish, and the culture dish is fixed.

Description

Culture apparatus for observing growth process of biological stem cells in each period in classified manner

Technical Field

The invention belongs to the technical field of cell culture, and particularly relates to a culture device for observing growth processes of biological stem cells in each period in a classified manner.

Background

The cell culture technology is an important and common technology in cell biology research methods, a large number of cells can be obtained through cell culture, signal transduction of the cells, anabolism of the cells, growth and proliferation of the cells and the like can be researched by the cell culture technology, each period of cell growth needs to be detected in order to better culture the cells, and the growth condition of the cells cannot be observed at any time when the cells are cultured by using the conventional culture box.

Reference can be made to patent with application number CN201510391262.X, which discloses an incubator technical field, discloses a cell incubator, including a box body and a front door movably connected with one side of the box body, the box body includes a cavity, at least two layers of clapboards are arranged in the cavity, the clapboards include a body and a support horizontally arranged, the support is connected with the body through a support rod, the horizontal height of the front end of the body is greater than the horizontal height of the rear end of the body; or the horizontal heights of the left end and the right end of the body are not consistent.

Based on the search of above-mentioned patent to and combine the equipment discovery among the prior art, the growth condition of observation cell that current cell culture case can not be at any time when cultivateing the cell, when adding nutrient solution to the culture dish, need open the apron of incubator, cause the inside temperature variation of incubator, influenced the growth of cell, cause the structure that detects inaccurate.

Therefore, in view of the above, the present invention provides a culture apparatus for observing the growth process of biological stem cells in each cycle by classification, so as to achieve the purpose of higher practical value.

Disclosure of Invention

In order to solve the technical problems, the invention provides a culture device for observing growth processes of biological stem cells in each period in a classified manner, so as to solve the problems that the growth condition of the cells cannot be observed at any time when the cells are cultured in the conventional cell culture box, and the cover plate of the culture box needs to be opened when nutrient solution is added to a culture dish, so that the temperature inside the culture box changes, the growth of the cells is influenced, and the detection structure is inaccurate.

The invention discloses a culture device for observing growth processes of biological stem cells in each period in a classified manner, which is realized by the following specific technical means: a culture device for observing growth processes of biological stem cells in each period in a classified manner comprises an incubator, a threaded rod, a connecting rod and a culture dish; the lower end of the incubator is fixedly connected with the H-shaped supporting plate, the right side of the upper end of the incubator is provided with a movable cover plate, and the left side of the upper end of the incubator is provided with an observation cylinder; a T-shaped groove is formed in the left side of the inner wall of the observation cylinder, and a through groove is formed in the right side of the observation cylinder; the culture dish is nested and installed in a groove on the upper surface of the rotating disc, two groups of limiting grooves are symmetrically arranged at the left end and the right end of the groove, and fixing devices are arranged in the limiting grooves; the lower end of the rotating disc is fixedly connected with the rotating shaft, and the lower end of the rotating shaft penetrates through the bottom plate of the incubator and is fixedly connected with the grooved pulley; the threaded rod penetrates through the H-shaped supporting plate and is fixedly connected with the hand wheel; the upper end of the connecting rod is fixedly connected with the bottom plate of the incubator, and the lower end of the connecting rod sequentially penetrates through the limiting disc and the rotating rod and is fixedly connected with the driven bevel gear; the side plate of the incubator is provided with a liquid conveying pipe, and the upper end of the liquid conveying pipe is provided with a feeding hole.

Furthermore, four groups of grooves are annularly arranged, and the groove on the left side is located right below the observation cylinder.

Furthermore, it is the back taper structure to observe a section of thick bamboo upper end, observes a section of thick bamboo lower extreme and is equipped with fixed convex mirror, and slides convex mirror left side and be equipped with T type piece, and T type piece sliding clip dress is in T type inslot portion, and the convex mirror right side that slides is equipped with adjusting screw, and adjusting screw sliding clip dress is in passing through the groove and fastening through lock nut.

Furthermore, the fixing device comprises a compression spring, a sliding plate and a clamping block, the sliding plate is slidably clamped in the limiting groove, the compression spring is arranged on the inner side of the sliding plate, the clamping block is arranged on the right side of the sliding plate, and the outer end of the clamping block is of an arc-shaped structure.

Furthermore, the outer surface of the feeding hole is provided with scale marks, the outer end of the infusion tube at the lower end of the feeding hole is provided with a valve, and the outlet of the infusion tube is positioned right above the groove.

Furthermore, a driving bevel gear is arranged on the outer surface of the threaded rod, and the driving bevel gear is meshed with the driven bevel gear.

Furthermore, four groups of sliding grooves are annularly arranged on the surface of the grooved wheel, a clamping column is arranged at the upper end of the rotating rod, the sliding of the clamping column is clamped in the sliding groove on the surface of the grooved wheel, and the limiting disc is attached to the outer wall of the grooved wheel.

Compared with the prior art, the invention has the following beneficial effects:

according to the invention, the sliding plate is slidably clamped in the limiting groove, so that when the culture dish is placed in the groove, the culture dish extrudes the fixture block into the limiting groove, the compression spring is stressed and compressed to provide a reaction force for the sliding plate, the fixture block at the front end of the sliding plate is tightly attached to the culture dish, the culture dish is fixed, the stability of the culture dish is enhanced, and the culture dish is prevented from shaking when the rotating disc rotates.

Through the setting of feed inlet outer end scale mark for when adding nutrient solution in to the culture dish, can guarantee to four culture dishes that the quantity that adds nutrient solution is the same, make the cell growth environment in four groups of culture dishes the same.

The setting of observing a section of thick bamboo directly over the culture dish, make the staff can observe the growth condition of the inside stem cell of culture dish through observing a section of thick bamboo, after having observed a culture dish, wave the hand wheel a round, because sheave surface annular is equipped with four groups sliding tray, when making the hand wheel drive threaded rod rotate the round, the rolling disc rotates the quarter round, adjacent culture dish rotates under observing a section of thick bamboo, make the staff can not just can accomplish the observation to the stem cell growth condition under the condition that the culture dish was taken out from the incubator inside, avoid the culture dish after taking out from the incubator inside, thereby cause harmful effects because the change of environment to the growth of stem cell, the accuracy of testing result has been guaranteed.

Through the setting of observing inside fixed convex mirror of a section of thick bamboo and slip convex mirror, make two convex mirrors arrange from top to bottom and can form a simple and easy microscope, the observation cost has been reduced, be convenient for use widely in the place that the condition is short of, still slide the setting of card dress in running through the groove and through the fastening of lock nut through adjusting screw, make and loosen the distance that can adjust between slip convex mirror and the fixed convex mirror after lock nut, when the workman sees the inside stem cell of culture dish, screw up lock nut and realize the fixed to the slip convex mirror, and is simple in operation, the practicality of the device has been strengthened.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention.

FIG. 2 is a schematic view showing the structure of the internal device of the incubator of the present invention.

Fig. 3 is a schematic sectional view of the rotating disk of the present invention.

Fig. 4 is an enlarged schematic view of the structure of fig. 3 a according to the present invention.

Figure 5 is a schematic view of the sheave structure of the present invention.

Fig. 6 is a schematic sectional structure view of the observation tube of the present invention.

Fig. 7 is a schematic view of the fixed convex mirror structure of the present invention.

Fig. 8 is a schematic view of the structure of the infusion tube of the present invention.

In the drawings, the corresponding relationship between the component names and the reference numbers is as follows:

1. an incubator; 101. a removable cover plate; 2. an H-shaped support plate; 3. a transfusion tube; 301. a feed inlet; 302. scale lines; 303. a valve; 4. a threaded rod; 401. a hand wheel; 402. a drive bevel gear; 5. a connecting rod; 501. a limiting disc; 502. rotating the rod; 5021. clamping the column; 503. a driven bevel gear; 6. a rotating shaft; 601. a grooved wheel; 7. rotating the disc; 701. a groove; 702. a limiting groove; 8. a culture dish; 9. an observation cylinder; 901. a T-shaped groove; 902. a through groove; 10. sliding the convex mirror; 1001. a T-shaped block; 1002. adjusting the screw rod; 11. a fixing device; 1101. a compression spring; 1102. a sliding plate; 1103. a clamping block; 12. the convex mirror is fixed.

Detailed Description

The embodiments of the present invention will be described in further detail with reference to the drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.

In the description of the present invention, "a plurality" means two or more unless otherwise specified; the terms "upper", "lower", "left", "right", "inner", "outer", "front", "rear", "head", "tail", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are only for convenience in describing and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, should not be construed as limiting the invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "connected" and "connected" are to be interpreted broadly, e.g., as being fixed or detachable or integrally connected; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Example (b):

as shown in figures 1 to 8:

the invention provides a culture device for observing growth processes of biological stem cells in each period in a classified manner, which comprises an incubator 1, a threaded rod 4, a connecting rod 5 and a culture dish 8; the lower end of the incubator 1 is fixedly connected with the H-shaped supporting plate 2, the right side of the upper end of the incubator 1 is provided with a movable cover plate 101, and the left side of the upper end of the incubator 1 is provided with an observation cylinder 9; a T-shaped groove 901 is formed in the left side of the inner wall of the observation cylinder 9, and a through groove 902 is formed in the right side of the observation cylinder 9; the culture dish 8 is nested and installed in a groove 701 on the upper surface of the rotating disc 7, two groups of limiting grooves 702 are symmetrically arranged at the left end and the right end of the groove 701, and fixing devices 11 are arranged in the limiting grooves 702; the lower end of the rotating disc 7 is fixedly connected with the rotating shaft 6, and the lower end of the rotating shaft 6 penetrates through the bottom plate of the incubator 1 and is fixedly connected with the grooved pulley 601; the threaded rod 4 penetrates through the H-shaped support plate 2 and is fixedly connected with a hand wheel 401; the upper end of the connecting rod 5 is fixedly connected with the bottom plate of the incubator 1, and the lower end of the connecting rod 5 sequentially passes through the limiting disc 501 and the rotating rod 502 and is fixedly connected with the driven bevel gear 503; the side plate of the incubator 1 is provided with a transfusion tube 3, and the upper end of the transfusion tube 3 is provided with a feeding hole 301.

Wherein, the recess 701 annular is equipped with four groups, and left recess 701 is located observation tube 9 under, in use, left recess 701 in rolling disc 7 upper end is located observation tube 9 under, make the staff can observe the growth condition of the inside stem cell of culture dish 8 through observation tube 9, and recess 701 annular is equipped with four groups, when making rolling disc 7 rotate quarter round, an adjacent group of recess 701 is located observation tube 9 under, make the staff can not observe the growth condition of stem cell under the condition that culture dish 8 was taken out from incubator 1 inside, thereby avoided culture dish 8 to cause harmful effects because of the change of environment to the growth of stem cell after taking out from incubator 1 inside, the accuracy of testing result has been guaranteed.

Wherein, the upper end of the observation tube 9 is a reverse taper structure, the lower end of the observation tube 9 is provided with a fixed convex lens 12, the left side of the sliding convex lens 10 is provided with a T-shaped block 1001, the T-shaped block 1001 is slidingly clamped inside a T-shaped groove 901, the right side of the sliding convex lens 10 is provided with an adjusting screw 1002, the adjusting screw 1002 is slidingly clamped in a through groove 902 and is fastened through a locking nut, in use, the upper end of the observation tube 9 is a reverse taper structure, which can prevent external light source from entering the inside of the observation tube 9 to cause light source pollution, thereby facilitating observation of workers, through the arrangement of the fixed convex lens 12 and the sliding convex lens 10 inside the observation tube 9, the two convex lenses are arranged up and down to form a simple microscope, thereby reducing observation cost and being convenient for popularization and use in places with deficient conditions, and the distance between the sliding convex lens 10 and the fixed convex lens 12 can be adjusted through the arrangement that the adjusting screw 1002 is slidingly clamped in the through groove 902 and is fastened through the locking nut after the locking nut is loosened, when the worker sees the stem cells inside the culture dish 8, the locking nut is screwed down to fix the sliding convex mirror 10.

Wherein, fixing device 11 includes compression spring 1101, sliding plate 1102 and fixture block 1103, sliding plate 1102 slide card dress is inside spacing groove 702, and the sliding plate 1102 inboard is equipped with compression spring 1101, the sliding plate 1102 right side is equipped with fixture block 1103, fixture block 1103 outer end is the arc structure, in use, the external diameter of culture dish 8 is less than the internal diameter of recess 701, in the time of rolling disc 7 rotation, culture dish 8 shakes easily, when culture dish 8 is placed inside recess 701, culture dish 8 extrudes fixture block 1103 inside spacing groove 702, thereby compression spring 1101 receives the force and compresses and gives a reaction force for sliding plate 1102, thereby make the fixture block 1103 and the culture dish 8 of sliding plate 1102 front end closely laminate, realized the fixed to culture dish 8, strengthened culture dish 8's stability.

Wherein, feed inlet 301 surface is equipped with scale mark 302, and the 3 outer ends of transfer line of feed inlet 301 lower extreme are equipped with valve 303, the export of transfer line 3 is located recess 701 directly over, in use, when cultivateing stem cell, need add the culture solution in the culture dish 8, can close valve 303, then pour the culture solution into feed inlet 301, feed inlet 301 outer end is provided with scale mark 302, can guarantee to add the quantity of nutrient solution the same to four culture dishes 8 at every turn, then open valve 303, the nutrient solution can enter into culture dish 8 through transfer line 3 inside, it is the same to have guaranteed that the growth environment in four groups of culture dishes 8 is the same.

Wherein, the surface of threaded rod 4 is equipped with drive bevel gear 402, and drive bevel gear 402 meshes with driven bevel gear 503 mutually, and in use, wave hand wheel 401 and drive the rotatory in-process of threaded rod 4, through the setting that drive bevel gear 402 meshes with driven bevel gear 503 mutually for the rotation of threaded rod 4 can drive the rotation of connecting rod 5.

Wherein, the annular of sheave 601 surface is equipped with four groups of sliding tray, and the dwang 502 upper end is equipped with card post 5021, the slip card of card post 5021 is adorned in the sliding tray on sheave 601 surface, and spacing dish 501 and the outer wall laminating of sheave 601, in use, the annular of sheave 601 surface is equipped with four groups of sliding tray, after dwang 502 rotated the round, sheave 601 rotated the quarter round, and sheave 601 is through pivot 6 and rolling disc 7 fixed connection, make rolling disc 7 also rotate the quarter round simultaneously, make the culture dish 8 of rolling disc 7 upper surface after rotating, observation cylinder 9 is located directly over culture dish 8 all the time, be convenient for workman's observation, the export of transfer line 3 is also directly over culture dish 8 all the time, be convenient for add nutrient solution inside culture dish 8.

The specific use mode and function of the embodiment are as follows:

in the invention, the movable cover plate 101 on the upper surface of the incubator 1 is opened, the culture dish 8 is placed in the groove 701 on the upper surface of the rotating disc 7, when the culture dish 8 is placed in the groove 701, the culture dish 8 extrudes the fixture block 1103 into the limiting groove 702, the compression spring 1101 is stressed and compressed to provide a reaction force for the sliding plate 1102, so that the fixture block 1103 at the front end of the sliding plate 1102 is tightly attached to the culture dish 8, the culture dish 8 is fixed, the stability of the culture dish 8 is enhanced, the culture dish 8 is prevented from shaking when the rotating disc 7 rotates, nutrient solution needs to be added to the culture dish 8 in the cell culture process, through the arrangement of the scale mark 302 at the outer end of the feed port 301, the same amount of nutrient solution added to the four culture dishes 8 every time can be ensured, the same growth environment in the four groups of culture dishes 8 is ensured, when the growth condition of cells in the culture dish 8 is observed, through the arrangement of the observation cylinder 9 right above the culture dish 8, a worker can observe the growth condition of stem cells in the culture dish 8 through the observation cylinder 9, after one culture dish 8 is observed, the hand wheel 401 is shaken for one circle, the threaded rod 4 is rotated to drive the connecting rod 5 to rotate through the arrangement of the engagement of the driving bevel gear 402 and the driven bevel gear 503, four groups of sliding grooves are annularly arranged on the surface of the grooved wheel 601, after the rotating rod 502 rotates for one circle, the grooved wheel 601 is driven to rotate for a quarter of a circle, and the grooved wheel 601 is fixedly connected with the rotating disc 7 through the rotating shaft 6, so that the rotating disc 7 also rotates for a quarter of a circle, the worker can complete the observation of the growth condition of the stem cells under the condition that the culture dish 8 is not taken out from the culture box 1, and the adverse effect on the growth of the stem cells due to the change of the environment is avoided after the culture dish 8 is taken out from the interior of the culture box 1, the accuracy of testing result has been guaranteed, through the setting of the inside fixed convex mirror 12 of observation section of thick bamboo 9 and slip convex mirror 10, make two convex mirrors arrange from top to bottom and can form a simple and easy microscope, the observation cost has been reduced, be convenient for use widely in the place that the condition is short of, still slide the card through adjusting screw 1002 and adorn in passing through groove 902 and through the setting of lock nut fastening, make and can adjust the distance between slip convex mirror 10 and the fixed convex mirror 12 after loosening lock nut, when the workman sees 8 inside stem cells of culture dish, it realizes fixing to slip convex mirror 10 to screw up lock nut, moreover, the steam generator is simple in operation, the practicality of this device has been strengthened.

The embodiments of the present invention have been presented for purposes of illustration and description, and are not intended to be exhaustive or limited to the invention in the form disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiment was chosen and described in order to best explain the principles of the invention and the practical application, and to enable others of ordinary skill in the art to understand the invention for various embodiments with various modifications as are suited to the particular use contemplated.

Claims

1. A culture apparatus for observing growth processes of biological stem cells in each period in a classified manner is characterized in that: comprises an incubator (1), a threaded rod (4), a connecting rod (5) and a culture dish (8); the lower end of the incubator (1) is fixedly connected with the H-shaped supporting plate (2), the right side of the upper end of the incubator (1) is provided with a movable cover plate (101), and the left side of the upper end of the incubator (1) is provided with an observation cylinder (9); a T-shaped groove (901) is formed in the left side of the inner wall of the observation cylinder (9), and a through groove (902) is formed in the right side of the observation cylinder (9); the culture dish (8) is nested and installed inside a groove (701) on the upper surface of the rotating disc (7), two groups of limiting grooves (702) are symmetrically arranged at the left end and the right end of the groove (701), and a fixing device (11) is arranged inside each limiting groove (702); the lower end of the rotating disc (7) is fixedly connected with the rotating shaft (6), and the lower end of the rotating shaft (6) penetrates through the bottom plate of the incubator (1) and is fixedly connected with the grooved pulley (601); the threaded rod (4) penetrates through the H-shaped support plate (2) and is fixedly connected with the hand wheel (401); the upper end of the connecting rod (5) is fixedly connected with the bottom plate of the incubator (1), and the lower end of the connecting rod (5) sequentially penetrates through the limiting disc (501) and the rotating rod (502) and is fixedly connected with the driven bevel gear (503); the side plate of the incubator (1) is provided with a perfusion tube (3), and the upper end of the perfusion tube (3) is provided with a feeding hole (301).

2. The culture device for observing growth processes of biological stem cells in each period in a classified mode according to claim 1, wherein: four groups of grooves (701) are annularly arranged, and the groove (701) on the left side is located right below the observation cylinder (9).

3. The culture device for observing growth processes of biological stem cells in each period in a classified mode according to claim 1, wherein: observe a section of thick bamboo (9) upper end and be the back taper structure, observe a section of thick bamboo (9) lower extreme and be equipped with fixed convex mirror (12), and slide convex mirror (10) left side and be equipped with T type piece (1001), T type piece (1001) slide clamping is inside T type groove (901), slide convex mirror (10) right side is equipped with adjusting screw (1002), adjusting screw (1002) slide clamping is in passing through groove (902) and through lock nut fastening.

4. The culture device for observing growth processes of biological stem cells in each period in a classified mode according to claim 1, wherein: the fixing device (11) comprises a compression spring (1101), a sliding plate (1102) and a clamping block (1103), the sliding plate (1102) is slidably clamped inside the limiting groove (702), the compression spring (1101) is arranged on the inner side of the sliding plate (1102), the clamping block (1103) is arranged on the right side of the sliding plate (1102), and the outer end of the clamping block (1103) is of an arc-shaped structure.

5. The culture device for observing growth processes of biological stem cells in each period in a classified mode according to claim 1, wherein: the outer surface of the feed port (301) is provided with scale marks (302), the outer end of the infusion tube (3) at the lower end of the feed port (301) is provided with a valve (303), and the outlet of the infusion tube (3) is positioned right above the groove (701).

6. The culture device for observing growth processes of biological stem cells in each period in a classified mode according to claim 1, wherein: the outer surface of the threaded rod (4) is provided with a driving bevel gear (402), and the driving bevel gear (402) is meshed with a driven bevel gear (503).

7. The culture device for observing growth processes of biological stem cells in each period in a classified mode according to claim 1, wherein: the surface of the grooved wheel (601) is annularly provided with four groups of sliding grooves, the upper end of the rotating rod (502) is provided with a clamping column (5021), the sliding clamp of the clamping column (5021) is arranged in the sliding groove on the surface of the grooved wheel (601), and the limiting disc (501) is attached to the outer wall of the grooved wheel (601).